Publications by authors named "B R Nagamalini"

6 Publications

Expression of β-catenin in oral leukoplakia and oral submucous fibrosis: An immunohistochemical study.

J Oral Maxillofac Pathol 2021 Jan-Apr;25(1):124-130. Epub 2021 May 14.

Department of Oral Pathology and Microbiology, A.E.C.S. Maaruti College of Dental Sciences and Research Centre, Bangalore, India.

Background: Oral potentially malignant disorders have increased propensity to turn malignant than its apparently normal counterparts. Histopathological examination, although gold standard, needs adjunct technique to give accurate diagnosis. Immunohistochemistry has proved to be a promising adjunct to aid in the diagnosis so far. The quest for a definitive marker is still on. Beta-catenin (β-catenin), a structural protein has been evaluated to identify its likely role in malignant transformation of potentially malignant lesions and possibly designate it as one of the identifiable signature molecules in the transformation.

Aim And Objective: To evaluate and estimate the expression of β-catenin in different grades of dysplasia, oral submucous fibrosis (OSMF) and normal mucosa and compare the same.

Methodology: A total number of 40 cases including different grades of dysplasia, OSMF and normal mucosa were immunohistochemically stained, location and intensity of its expression were evaluated for β-catenin. The results were statistically analyzed using the one-way analysis of variance and Chi-square test.

Results: The expression of β-Catenin in the cytoplasm as well as in the nucleus increased from mild-to-moderate dysplasia to OSMF and to severe epithelial dysplasia in an increasing order. The expression is seen to translocate from membranous to cytoplasm to nucleus indicating a proliferative potential in these group of lesions.

Conclusion: β-catenin is a promising marker which indicates the malignant transformation potential in the higher grades of dysplasia and OSMF.
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May 2021

Coalition of E-cadherin and vascular endothelial growth factor expression in predicting malignant transformation in common oral potentially malignant disorders.

J Oral Maxillofac Pathol 2018 Jan-Apr;22(1):40-47

Department of Oral and Maxillofacial Pathology, AECS Maaruti College of Dental Sciences and Research Centre, Bengaluru, Karnataka, India.

Background: Reduced E-cadherin expression and increased VEGF expression is known to be involved in tissue growth and transformation of Oral Potentially Malignant Disorders (OPMDs) and has been correlated with their differing histological grades in numerous studies.

Aim: To evaluate Immunohistochemical (IHC) expression of both E-cadherin and VEGF in predicting the malignant transformation potential of common OPMDs.

Materials And Methods: Ten cases each of Normal Oral mucosa (NOM), Mild Oral Epithelial Dysplasia (OED), Moderate OED, Severe OED, Oral Submucous Fibrosis, (OSMF) and Oral Squamous Cell Carcinoma (OSCC) were stained and evaluated for the expression of Ecadherin and VEGF. Quick score (QS) for expression intensity in all epithelial layers was calculated for both markers and results statistically analysed using Kruskal -Wallis ANOVA and Mann-Whitney "U" test.

Results: E-cadherin expression was continuous and membranous in all the layers of NOM and reduced with progressing grades of OED to OSCC. In OSMF, expression was intermediate between moderate and severe OED. VEGF expression increased as the disease progressed from normal to increasing grades of OED to malignancy. In OSMF, expression was similar to that in mild OED. VEGF, E-cadherin expression for basal and parabasilar cells showed a strong statistically significant negative correlation in NOM. A very strong statistically significant positive correlation with perfect monotonic relation was noted in superficial cells in severe OED group and OSCC group.

Conclusion: E-Cadherin and VEGF could be used as combination markers to predict the potential risk for malignant transformation in OEDs.
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May 2018

Beta-catenin in disease.

J Oral Maxillofac Pathol 2016 May-Aug;20(2):289-99

Department of Oral and Maxillofacial Pathology, AECS Maaruti College of Dental Sciences and Research Center, Bengaluru, Karnataka, India.

In continuation with the previous review on "β-catenin in health", in this review we discuss the role of β-catenin in the pathogenesis of common oral lesions in the oral and maxillofacial region- oral potentially malignant disorders, their progression to oral squamous cell carcinoma, salivary gland tumors and odontogenic tumours. This review is based on a pubmed search of all the lesions included in the review.
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September 2016

Immunohistochemical analysis of laminin expression in adenoid cystic carcinoma.

J Oral Maxillofac Pathol 2014 Sep;18(Suppl 1):S26-31

Department of Oral and Maxillofacial Pathology, Amrith Educational and Cultutal Society Maaruti College of Dental Sciences and Research Centre, Bengaluru, Karnataka, India.

Background And Objectives: This study aims at the observation of the immunohistochemical expression of laminin in adenoid cystic carcinoma (ACC) of salivary gland origin and to analyze the distribution of laminin in various components of the tumor and correlate the expression of laminin with the growth and differentiation of the tumor.

Materials And Methods: THIRTY CASES OF ACC WERE SUBJECTED TO IMMUNOHISTOCHEMICAL STUDY USING POLYCLONAL ANTIHUMAN LAMININ PRIMARY ANTIBODY, DISTRIBUTION OF LAMININ IN EACH CASE OF ACC WAS OBSERVED IN THE FOLLOWING AREAS: Intracellularly, inner borders of the pseudocystic spaces, within the lumen of the pseudocysts, around the tumor islands and in the intervening stroma.

Results: Laminin positivity was observed in the inner aspect of the pseudocystic spaces in 15 cases, within the lumen of pseudocystic spaces in 22 cases, in the intervening stroma in 20 cases, bordering the tumor islands in 16 cases and intracellularly in 4 cases.

Interpretation And Conclusion: Based on these observations, it can be assumed that laminin plays a major role in proliferation of the tumor cells and in pseudocyst formation. Thus, laminin might play a significant role in the growth and differentiation of ACC and also help in assessing the prognosis of the tumor.
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September 2014

Evaluation and comparison of expression of p63 in odontogenic keratocyst, solid ameloblastoma and unicystic ameloblastoma.

J Oral Maxillofac Pathol 2014 May;18(2):223-8

Department of Oral and Maxillofacial Pathology, AECS Maruti College of Dental Sciences and Research Centre, Bangalore, Karnataka, India.

Background And Objectives: The behavior of odontogenic lesions varies with some tumors behaving like a cyst and some cysts behaving like tumors. p63, a member of the p53 family of tumor suppressor genes has recently come into light in view of its role as an oncogene. The aim of the present study was to investigate the expression of p63 protein in OKC, Solid ameloblastoma, Unicystic Ameloblastoma and Follicular tissue.

Materials And Methods: p63 expression was compared in 12 cases of OKC, 12 Solid Ameloblastoma, 14 cases of Unicystic ameloblastoma and 10 cases of Follicular tissue using immunohistochemical technique. All 48 cases were subjected to heat-induced antigen retrieval method using citrate buffer in a pressure cooker. Then the sections were stained with anti-p63 polyclonal antibody and visualized using super sensitive polymer HRP detection system. In each case, number of cells showing p63 positivity were assessed in two compartments - basal and suprabasal and compared.

Results: Statistical analysis showed that p63 expression in the suprabasal compartment in Odontogenic keratocysts was equivalent to that of central neoplastic cells of Solid Ameloblastoma and Unicystic Ameloblastoma type 3. Statistically significant difference in the expression of p63 was observed between OKC and Unicystic Ameloblastoma Type 1 and Solid Ameloblastoma and Unicystic Ameloblastoma Type 1.

Conclusion: We conclude that the higher expression of p63 in these odontogenic lesions correlates well with their aggressive behavior and thereby suggesting alterations in treatment modalities.
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May 2014