Publications by authors named "Awatef Bejaoui"

2 Publications

  • Page 1 of 1

Prevalence and Antibiotic Resistance Patterns of spp. Isolated from Broiler Chickens in the North of Tunisia.

Biomed Res Int 2018 23;2018:7943786. Epub 2018 Dec 23.

Laboratory of Epidemiology and Veterinary Microbiology, Group of Bacteriology and Biotechnology Development, Institut Pasteur de Tunis, University of Tunis El Manar (UTM), BP 74, 13 Place Pasteur, Belvédère, 1002 Tunis, Tunisia.

The aim of the current study is to assess the prevalence of infection in broiler chickens, raised in intensive production conditions, and to evaluate the antimicrobial susceptibility of recovered isolates. A total of 590 cloacal swab samples were taken from 13 broiler chicken flocks in the North East of Tunisia. All samples were tested for the presence of thermophilic by culture and PCR, targeting the and genes, respectively. Susceptibility to antimicrobial drugs was tested against 8 antibiotics. Prevalence of infection, relationship with geographic origins and seasons, antimicrobial resistance rates and patterns were analyzed. Total prevalence of infection in broiler flocks was in the range of 22.4%, with a predominance of (68.9%), followed by (31.1%). Positive association was highlighted between the infection level and the season ( < 0.001), but no link was emphasized considering the geographic origin. Antimicrobial susceptibility testing revealed very high resistance rates detected against macrolide, tetracycline, quinolones, and chloramphenicol, ranging from 88.6% to 100%. Lower resistance prevalence was noticed for -lactams (47% and 61.4%) and gentamicin (12.9%). 17 R-type patterns were observed, and a common pattern was found in 30.3% of isolates. This study provides updates and novel data on the prevalence and the AMR of broiler campylobacters in Tunisia, revealing the occurrence of high resistance to several antibiotics and emphasizing the requirement of better surveillance and careful regulation of antimicrobials use.
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May 2019

Molecular characterization of Coxiella burnetii isolates by infrequent restriction site-PCR and MLVA typing.

BMC Microbiol 2006 Apr 26;6:38. Epub 2006 Apr 26.

INRA, Pathologie Infectieuse et Immunologie, 37380 Nouzilly, France.

Background: Coxiella burnetii, the causative agent of Q fever, has a wide host range. Few epidemiological tools are available, and they are often expensive or not easily standardized across laboratories. In this work, C. burnetii isolates from livestock and ticks were typed using infrequent restriction site-PCR (IRS-PCR) and multiple loci variable number of tandem repeats (VNTR) analysis (MLVA).

Results: By applying IRS-PCR, 14 C. burnetii isolates could be divided into six groups containing up to five different isolates. Clustering as deduced from MLVA typing with 17 markers provided an increased resolution with an excellent agreement to IRS-PCR, and with the plasmid type of each strain. MLVA was then applied to 28 additional C. burnetii isolates of different origin and 36 different genotypes were identified among the 42 isolates investigated. The clustering obtained is in agreement with published Multiple Locus Sequence Typing (MLST) data. Two panels of markers are proposed, panel 1 which can be confidently typed on agarose gel at a lower cost and in any laboratory setting (10 minisatellite markers with a repeat unit larger than 9 bp), and panel 2 which comprises 7 microsatellites and provides a higher discriminatory power.

Conclusion: Our analyses demonstrate that MLVA is a powerful and promising molecular typing tool with a high resolution and of low costs. The consistency of the results with independent methods suggests that MLVA can be applied for epidemiological studies. The resulting data can be queried on a dedicated MLVA genotyping Web service.
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April 2006