Babasaheb Bhimrao Ambedkar University
Lucknow, Uttar Pradesh | India
Main Specialties: Biotechnology, Endocrinology Diabetes & Metabolism, Infectious Disease, Medical Toxicology, Pharmacology, Pulmonary Disease & Critical Care Medicine
Additional Specialties: NMR based Metabolomics
My research interest includes the exploratory application of NMR spectroscopy in early detection of Human Disease Biomarkers, Drug Metabolism and Toxicity, Cancer Metabolism, Immunology, and Neuroscience.
Primary Affiliation: Babasaheb Bhimrao Ambedkar University - Lucknow, Uttar Pradesh , India
PubMed Central Citations
49PubMed Central Citations
Front Pharmacol 2018 6;9:314. Epub 2018 Apr 6.
Department of Pharmaceutical Sciences, School of Biosciences and Biotechnology, Babasaheb Bhimrao Ambedkar University (A Central University), Lucknow, India.
Int J Biochem Cell Biol 2018 Mar 2;96:51-62. Epub 2018 Feb 2.
Department of Pharmaceutical Sciences, Babasaheb Bhimrao Ambedkar University (A Central University), Vidya Vihar, Raebareli Road, Lucknow, 226025 UP, India. Electronic address:
Chem Biol Interact 2018 Jan 6;280:33-44. Epub 2017 Dec 6.
Department of Pharmaceutical Sciences, Babasaheb Bhimrao Ambedkar University, Vidya Vihar, Raebareli Road, Lucknow 226025, Uttar Pradesh, India. Electronic address:
Rawat A, Srivastava RK, Dubey D, Guleria A, Singh S, Prakash A, Modi DR, Khetrapal CL, Tiwari S, Kumar D. Serum metabolic disturbances hailing in initial hours of acute myocardial infarction elucidated by NMR based metabolomics. Current Metabolomics. 2017 Apr 1;5(1):55-67.
Background: Acute myocardial infarction (AMI) is a serious medical emergency and leading cause of cardiac-related deaths worldwide. The devastating outcome is sudden death of the patient within first few hours from the onset of symptoms. The rapid detection of physiological transformations associated with AMI coupled with instant treatment to reset these changes and monitoring response to treatment can greatly decrease the mortality and morbidity of patients. Objective: To establish the early hour metabolomic signatures in the sera of AMI patieints. Methodology: Metabolic profiles of sera collected from 42 AMI patients (immediately after the myocardial infarction) and 38 age/sex matched normal controls were obtained using high-resolution 1D 1H CPMG and diffusion edited NMR spectra. The metabolic profiles were compared using multivariate statistical analysis to identify the disease specific metabolic disturbances associated with AMI and, therefore, the perturbed biochemical pathways in this condition. Results: Our results revealed significant metabolic perturbations in AMI compared to control cohorts. The upregulated metabolites in AMI condition include arginine, glycine, tyrosine, phenylalanine, glucose, creatine, creatinine, lactate, N-acetyl glycoproteins and phospholipids, while the levels of amino acids (such as valine, alanine, glutamate, glutamine, threonine and methionine), citrate, acetone, choline, glycero-phosphocholine, trimethylamine-N-oxide and lipids/fatty acids were decreased. Receiver operating curve characteristics (ROC) confirmed the robustness and validity of these metabolic markers. Conclusion: The resulted metabolic profiling provided new insights into the metabolic processes involved in acute myocardial infarction. Further, we foresee that these changes would aid rapid clinical evaluation of myocardial infarction in emergency and its timely management.