Publications by authors named "Asumi Fukunaga"

3 Publications

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Intraspecific variations of the cytoplasmic male sterility genes and in Brassica maurorum and Moricandia arvensis, and the specificity of the mRNA processing.

Genome 2021 Jun 15. Epub 2021 Jun 15.

Dept. Biotech., Fac. Eng., Kyoto Sangyo Univ., Motoyama, Kamigamo, Kyoto, Kyoto, Japan, 603-8555;

The mitochondrial gene co-transcribed with and causes cytoplasmic male sterility in crops, is widely distributed across wild species and genera of . However, intraspecific variations in the presence of have not yet been studied, and the mechanisms for the wide distribution of the gene remain unclear. We analyzed the presence and sequence variations of in two wild species, and . After polymerase chain reaction amplification of the 5' region of and the coding sequence of , we determined the DNA sequences. and showed variations for the presence of or () both between and within accessions, and were not fixed to the mitochondrial type having the male sterile genes. Sequencing of the amplicons clarified that has instead of . Sequencing also indicated mitochondrial heteroplasmy in the two species; particularly, in , one plant possessed both the and sequences. The results suggested that substoichiometric shifting of the mitochondrial genomes leads to the acquisition or loss of . Furthermore, fertility restorer genes of the two species were involved in the processing of the mRNA of the male sterility genes at different sites.
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http://dx.doi.org/10.1139/gen-2021-0011DOI Listing
June 2021

A single nucleotide substitution in the coding region of Ogura male sterile gene, orf138, determines effectiveness of a fertility restorer gene, Rfo, in radish.

Mol Genet Genomics 2021 May 26;296(3):705-717. Epub 2021 Mar 26.

Faculty of Life Sciences, Kyoto Sangyo University, Kamigamo, Kita, Kyoto, 603-8555, Japan.

Cytoplasmic male sterility (CMS) observed in many plants leads defect in the production of functional pollen, while the expression of CMS is suppressed by a fertility restorer gene in the nuclear genome. Ogura CMS of radish is induced by a mitochondrial orf138, and a fertility restorer gene, Rfo, encodes a P-type PPR protein, ORF687, acting at the translational level. But, the exact function of ORF687 is still unclear. We found a Japanese variety showing male sterility even in the presence of Rfo. We examined the pollen fertility, Rfo expression, and orf138 mRNA in progenies of this variety. The progeny with Type H orf138 and Rfo showed male sterility when their orf138 mRNA was unprocessed within the coding region. By contrast, all progeny with Type A orf138 were fertile though orf138 mRNA remained unprocessed in the coding region, demonstrating that ORF687 functions on Type A but not on Type H. In silico analysis suggested a specific binding site of ORF687 in the coding region, not the 5' untranslated region estimated previously, of Type A. A single nucleotide substitution in the putative binding site diminishes affinity of ORF687 in Type H and is most likely the cause of the ineffectiveness of ORF687. Furthermore, fertility restoration by RNA processing at a novel site in some progeny plants indicated a new and the third fertility restorer gene, Rfs, for orf138. This study clarified that direct ORF687 binding to the coding region of orf138 is essential for fertility restoration by Rfo.
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http://dx.doi.org/10.1007/s00438-021-01777-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144145PMC
May 2021

Appearance of male sterile and black radishes in the progeny of cross between and .

Breed Sci 2020 Dec 5;70(5):637-641. Epub 2020 Nov 5.

Faculty of Life Sciences, Kyoto Sangyo University, Kamigamo, Kita, Kyoto 603-8555, Japan.

In addition to Ogura cytoplasmic male sterility (CMS), which is used extensively for F hybrid seed production in Brassicaceae crops, two other CMS systems, NWB CMS and DCGMS, have also been identified. The causal gene for the latter two CMS systems has been identified as a novel chimeric gene, . We previously reported that is specific to black radish cultivars and that it is present in line 'RS-5' of ; however, the sequence in 'RS-5' differed from that of black radish cultivars. Though, with an sequence identical to that found in black radish cultivars was recently identified. We therefore sought to determine whether the gene in line 'RS-5' induces CMS in radishes. We crossed 'RS-5' as a female parent with a cultivated radish, 'Uchiki-Gensuke', as a male parent, and examined the gross plant morphology and pollen fertility of the resulting progeny. The F population contained both male sterile plants and plants with black roots. The findings showed that contains two types of genes that induce CMS, and that the origin of black radishes could be attributed to having gene.
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http://dx.doi.org/10.1270/jsbbs.20081DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7878931PMC
December 2020
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