Publications by authors named "Ashok Kumar Patra"

9 Publications

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Preparation of novel biodegradable starch/poly(vinyl alcohol)/bentonite grafted polymeric films for fertilizer encapsulation.

Carbohydr Polym 2021 May 22;259:117679. Epub 2021 Jan 22.

Division of Environmental Soil Science, ICAR-Indian Institute of Soil Science, Bhopal, 462038, India. Electronic address:

Sufficient hydroxyl moiety, ease of accessibility, biodegradability and reaction compatibility with other molecules make starch a basic ingredient for polymeric synthesis and to prepare encapsulated controlled release fertilizers. This article aims to prepare biodegradable clay-polymeric (starch/PVA) blended encapsulating films (CPSBs) from starch/PVA and economically feasible clay-fractioned bentonite for CPSB-encapsulated diammonium phosphate (DAP) production. The XRD, TEM and FTIR spectroscopy recognized the compatibility of bentonite with starch/PVA blend; several micropores in CPSB surface was visible through SEM. Relative crystallinity index, density of CPSBs increased with increasing bentonite content (0-20 wt%); but, porosity, water absorption was decreased. Half-life of CPSB-10 was 37.4, 40.1 and 51.9 days with Aspergillus awamori, Trichoderma viride and uninoculated soil, respectively. Nitrogen (N) and phosphorus (P) release data from CPSB-encapsulated-DAP and uncoated DAP fitted well to Korsmeyer-Peppas model. Overall, greater bentonite content stabilizes the CPSB structure and CPSB-encapsulation reduced the N and P release from DAP.
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http://dx.doi.org/10.1016/j.carbpol.2021.117679DOI Listing
May 2021

Isolation of a broad spectrum antimicrobial producing thermophilic Bacillus and characterization of its antimicrobial protein.

Arch Microbiol 2021 Jul 11;203(5):2059-2073. Epub 2021 Feb 11.

Department of Botany and Biotechnology, Ravenshaw University, Cuttack, Odisha, India, 753003.

The hot spring water of Atri in India was believed to have disease curing property. An antibacterial producing organism was isolated and identified as Bacillus paralicheniformis by morphology, microscopy, and 16S-rRNA. Its secretion inhibited bacteria, yeast, and fungus in well-diffusion-method. The secreted antimicrobial was a 16.74 kDa protein homologous of chicken-lysozyme-C. The novel lysozyme's activities were recorded under different parameters. It was active from pH 5-9 and endured up to 60 °C for 120 min. Complete cell wall lysis of S. flexneri and P. aeruginosa was observed under a microscope at 4500× with a minimum inhibitory concentration of 7.8 µg/ml, while others required a higher dose, i.e., 13 µg/ml, and 20 µg/ml for E.coli and S. typhimurium, respectively. The discovered lysozyme has the extraordinary potential to lyse Gram-positive bacteria, yeast, fungus, and more efficiently lyse chick-lysozyme-C resistant lipopolysaccharide rich Gram-negative bacteria's outer cell wall.
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http://dx.doi.org/10.1007/s00203-020-02162-wDOI Listing
July 2021

Effects of Co-composting of Municipal Solid Waste and Pigeon Pea Biochar on Heavy Metal Mobility in Soil and Translocation to Leafy Vegetable Spinach.

Bull Environ Contam Toxicol 2021 Mar 27;106(3):536-544. Epub 2021 Jan 27.

ICAR-Indian Institute of Soil Science, Nabibagh, Bhopal, Madhya Pradesh, 462 038, India.

An experiment was conducted to study the effects of co-composted products of municipal solid waste (MSW) and pigeon pea biochar (PPB) on heavy metal mobility in soil and its uptake by spinach. Application of municipal solid waste biochar co-compost (MSWBC) significantly (p ≤ 0.05) reduced the heavy metal content in spinach leaves and roots compared to municipal solid waste compost (MSWC) amended soil. The percent decrease in spinach leaf following the application of MSWBC-10% PPB compared to MSWC was 20.62%, 28.95%, 36.02%, 41.88%, 41.50%, and 41.23% for Cu, Cd, Pb, Cr, Ni, and Zn, respectively. The dry matter yield of spinach and soil organic carbon (SOC) content in soil amended with MSWBC-10% PPB was significantly increased by 32.75% and 47.73%; and 17.62% and 27.45% relative to control and MSWC amended soil. The study concludes that co-composted product, MSWBC, stabilized heavy metals in MSW, reduced their uptake by spinach and thus making it a viable option for safe disposal of MSW.
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http://dx.doi.org/10.1007/s00128-020-03096-1DOI Listing
March 2021

Evaluation of Furcraea foetida (L.)Haw. for phytoremediation of cadmium contaminated soils.

Environ Sci Pollut Res Int 2021 Mar 25;28(11):14177-14181. Epub 2021 Jan 25.

ICAR-Indian Institute of Soil Science, NabiBagh, Berasia Road, Bhopal, 462038, India.

In the present study, we evaluated Furcraea foetida for the phytoremediation of cadmium (Cd)-contaminated soils. We selected F. foetida because it is a drought-resistant plant, produces high biomass, and needs minimum maintenance. It belongs to the leaf fiber group of plants and therefore has economic importance. Since it is a non-edible crop, there is no danger of food chain contamination. Despite possessing these ideal characteristics, surprisingly, to date, the plant is underutilized for phytoremediation purposes. Therefore, to evaluate the phytoremediation potential of the plant, we exposed it to five levels of cadmium (0, 25, 50, 100, and 200 mg Cd kg soil) and studied its influence on growth, dry matter production, uptake, and translocation efficiency. The plant showed good tolerance to Cd 200 mg kg soil without exhibiting any visible toxicity symptoms. The metal mainly accumulated in the roots (233 μg gdw), followed by leaf (51 μg g dw). The bioconcentration factor was > 1, but the translocation factor was < 1. The plant was not classified as a hyperaccumulator of Cd; however, because of its high uptake (897 μ g plant) and translocation efficiency (78%), we concluded that the plant could be utilized for phytoextraction of Cd from soils with low to moderately contaminated soils.
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http://dx.doi.org/10.1007/s11356-021-12534-4DOI Listing
March 2021

Classification of Pigeonpea ( (L.) Millsp.) Genotypes for Zinc Efficiency.

Plants (Basel) 2020 Jul 28;9(8). Epub 2020 Jul 28.

International Zinc Association, New Delhi 110062, India.

Pigeonpea ( (L.) Millsp.) is grown globally for its protein-rich seed. However, low availability of soil zinc (Zn) adversely affects the seed yield of pigeonpea. The present study was therefore conducted to assess the Zn efficiency of pigeonpea genotypes based on seed yield and seed Zn uptake efficiency. Field experiments were conducted at the Indian Council of Agricultural Research-Indian Institute of Soil Science, Bhopal, India with twenty different pigeonpea genotypes and two levels of Zn application under a split-plot design. The two levels of Zn were low (without application of Zn fertilizer) and high (with application of 20 kg Zn ha (as ZnSO∙7H2O) as basal soil application, in conjunction with three foliar sprays of 0.50% (/) ZnSO∙7HO aqueous solution) (with 0.25% lime as neutralizing agent) at flowering, pod formation, and pod filling stages). Application of Zn improved plant height, branches plant, pods plant, seeds pod, and 100 seed weight of pigeonpea genotypes differently. The mean seed yield, seed Zn concentration, and seed Zn uptake of the genotypes increased from 1.71 to 2.12 t ha, 32.4 to 43.0 mg kg, and 54.9 to 90.6 g ha, respectively, with application of Zn. The seed yield efficiency index (SYEI) and Zn uptake efficiency index (ZUEI) of pigeonpea genotypes varied from 67.0 to 92.5 and from 47.0 to 69.9, respectively. Based on SYEI and ZUEI, the genotypes were classified as efficient and responsive (Virsa Arhar-1, GT-1, GT-101, SKNP 05-05, BDN-2, AAUT 2007-04, BSMR 853, T 15-15, DT 23, Pusa 9), efficient and non-responsive (ICPL 87119, PKV Trombay), inefficient and responsive (AKT 8811, Hisar Paras), and inefficient and non-responsive (AAUT 2007-10, JKM 7, Hisar Manak, C 11, Hisar HO2-60, GAUT 93-17). The efficient and responsive genotypes are the most useful as they yield well under low soil Zn conditions and also respond to Zn fertilizer application. The inefficient and responsive genotypes could be utilized for plant breeding programs by plant breeders for identification and utilization of responsive traits.
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http://dx.doi.org/10.3390/plants9080952DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7463768PMC
July 2020

Effect of Soil Amendments on Microbial Resilience Capacity of Acid Soil Under Copper Stress.

Bull Environ Contam Toxicol 2017 Nov 9;99(5):625-632. Epub 2017 Sep 9.

ICAR-Indian Institute of Soil Science, Nabibagh, Berasia Road, Bhopal, 462038, India.

An incubation study was undertaken to study microbial resilience capacity of acid soil amended with farmyard manure (FYM), charcoal and lime under copper (Cu) perturbation. Copper stress significantly reduced enzymatic activities and microbial biomass carbon (MBC) in soil. Percent reduction in microbial activity of soil due to Cu stress was 74.7% in dehydrogenase activity, 59.9% in MBC, 48.2% in alkaline phosphatase activity and 15.1% in acid phosphatase activity. Soil treated with FYM + charcoal showed highest resistance index for enzymatic activities and MBC. Similarly, the highest resilience index for acid phosphatase activity was observed in soil amended with FYM (0.40), whereas FYM + charcoal-treated soil showed the highest resilience indices for alkaline, dehydrogenase activity and MBC: 0.50, 0.22 and 0.25, respectively. This investigation showed that FYM and charcoal application, either alone or in combination, proved to be better than lime with respect to microbial functional resistance and resilience of acid soil under Cu perturbation.
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http://dx.doi.org/10.1007/s00128-017-2173-8DOI Listing
November 2017

Phylloplane bacteria of Jatropha curcas: diversity, metabolic characteristics, and growth-promoting attributes towards vigor of maize seedling.

Can J Microbiol 2017 Oct 31;63(10):822-833. Epub 2017 Jul 31.

Indian Institute of Soil Science, Nabibagh, Bhopal 462038, India.

The complex role of phylloplane microorganisms is less understood than that of rhizospheric microorganisms in lieu of their pivotal role in plant's sustainability. This experiment aims to study the diversity of the culturable phylloplane bacteria of Jatropha curcas and evaluate their growth-promoting activities towards maize seedling vigor. Heterotrophic bacteria were isolated from the phylloplane of J. curcas and their 16S rRNA genes were sequenced. Sequences of the 16S rRNA gene were very similar to those of species belonging to the classes Bacillales (50%), Gammaproteobacteria (21.8%), Betaproteobacteria (15.6%), and Alphaproteobacteria (12.5%). The phylloplane bacteria preferred to utilize alcohol rather than monosaccharides and polysaccharides as a carbon source. Isolates exhibited ACC (1-aminocyclopropane-1-carboxylic acid) deaminase, phosphatase, potassium solubilization, and indole acetic acid (IAA) production activities. The phosphate-solubilizing capacity (mg of PO solubilized by 10 cells) varied from 0.04 to 0.21. The IAA production potential (μg IAA produced by 10 cells in 48 h) of the isolates varied from 0.41 to 9.29. Inoculation of the isolates to maize seed significantly increased shoot and root lengths of maize seedlings. A linear regression model of the plant-growth-promoting activities significantly correlated (p < 0.01) with the growth parameters. Similarly, a correspondence analysis categorized ACC deaminase and IAA production as the major factors contributing 41% and 13.8% variation, respectively, to the growth of maize seedlings.
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http://dx.doi.org/10.1139/cjm-2017-0189DOI Listing
October 2017

Aquatic microphylla Azolla: a perspective paradigm for sustainable agriculture, environment and global climate change.

Environ Sci Pollut Res Int 2016 Mar 23;23(5):4358-69. Epub 2015 Dec 23.

Indian Institute of Soil Science (IISS), Berasia Road, Nabibagh, Bhopal, MP, India, 462038.

This review addresses the perspectives of Azolla as a multifaceted aquatic resource to ensure ecosystem sustainability. Nitrogen fixing potential of cyanobacterial symbiont varies between 30 and 60 kg N ha(-1) which designates Azolla as an important biological N source for agriculture and animal industry. Azolla exhibits high bioremediation potential for Cd, Cr, Cu, and Zn. Azolla mitigates greenhouse gas emission from agriculture. In flooded rice ecosystem, Azolla dual cropping decreased CH4 emission by 40 % than did urea alone and also stimulated CH4 oxidation. This review highlighted integrated approach using Azolla that offers enormous public health, environmental, and cost benefits.
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http://dx.doi.org/10.1007/s11356-015-5857-9DOI Listing
March 2016

RBx-0597, a potent, selective and slow-binding inhibitor of dipeptidyl peptidase-IV for the treatment of type 2 diabetes.

Eur J Pharmacol 2011 Feb 9;652(1-3):157-63. Epub 2010 Jun 9.

Department of Pharmacology, New Drug Discovery Research, Ranbaxy Research Laboratories, Gurgaon, Haryana-122001, India.

Dipeptidyl peptidase IV (DPP-IV) inhibiton is a well recognized approach to treat Type 2 diabetes. RBx-0597 is a novel DPP-IV inhibitor discovered in our laboratory. The aim of the present study was to characterize the pharmacological profiles of RBx-0597 in vitro and in vivo as an anti-diabetic agent. RBx-0597 inhibited human, mouse and rat plasma DPP-IV activity with IC(50) values of 32, 31 and 39nM respectively. RBx-0597 exhibited significant selectivity over dipeptidyl peptidase8 (DPP-8), dipeptidyl peptidase9 (DPP-9) (150-300 fold) and other proline-specific proteases (>200-2000 fold). Kinetic analysis revealed that RBx-0597 is a competitive and slow binding DPP-IV inhibitor. In ob/ob mice, RBx-0597 (10mg/kg) inhibited plasma DPP-IV activity upto 50% 8h post-dose and showed a dose-dependent glucose excursion. RBx-0597 (10mg/kg) showed a significant glucose lowering effect (∼25% AUC of △ blood glucose) which was sustained till 12h, significantly increased the active glucagon-like peptide-1(GLP-1) and insulin levels. It showed a favourable pharmacokinetic profile (plasma clearance:174ml/min/kg; C(max) 292ng/ml; T(1/2) 0.28h; T(max) 0.75h and V(ss) 4.13L/kg) in Wistar rats with the oral bioavailability (F(oral)) of 65%. In summary, the present studies indicate that RBx-0597 is a novel DPP-IV inhibitor with anti-hyperglycemic effect and a promising candidate for further development as a drug for the treatment of type 2 diabetes.
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http://dx.doi.org/10.1016/j.ejphar.2010.06.001DOI Listing
February 2011
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