Publications by authors named "Asghar Ashrafi Hafez"

13 Publications

  • Page 1 of 1

Evaluation of expression of vitamin D receptor related lncRNAs in lung cancer.

Noncoding RNA Res 2020 Sep 24;5(3):83-87. Epub 2020 May 24.

Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Lung cancer as the most common cancer in the world is associated with high rate of mortality. Previous studies have detected expression of vitamin D receptor (VDR) in lung cancer tissues and reported significant of this gene in determination of patients' survival. Methods: In the current study, we assessed expression of and five long non-coding RNAs (lncRNAs) which have been associated with VDR (, , , ) in 32 pairs of lung cancer tissues and adjacent non-cancerous tissues (ANCTs) using real time PCR method. Expression of was significantly decreased in tumor tissues obtained from male patients compared with their matched ANCTs (ER = 0.31, P value = 0.02). However, this pattern was not detected in female subjects (ER = 0.93, P value = 0.94). Expression of LINC00346 was significantly decreased in tumoral tissues compared with ANCTs (Expression ratio (ER) = 0.38, P value = 0.03). When evaluating expression of this lncRNA based on the sex of patients, differences in its expression was only significant among males (ER = 0.3, P value = 0.04). expression was significantly associated with sex of patients in a way that most male patients exhibited down-regulation of this gene in their tumor tissue samples compared with the paired ANCTs (P = 0.03). Expression levels of LINC00346 could discriminate lung cancer tissues from ANCTs with sensitivity of 83.3% and specificity of 52.4%. Correlations between expressions of and other genes were all significant in tumoral tissues but insignificant in ANCTs. The current investigation potentiates and LINC00346 as possible participants in the pathogenesis of lung cancer.
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http://dx.doi.org/10.1016/j.ncrna.2020.05.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7264462PMC
September 2020

Expression assessment of a panel of long non-coding RNAs in gastric malignancy.

Exp Mol Pathol 2020 04 23;113:104383. Epub 2020 Jan 23.

Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address:

Background: Long non-coding RNAs (lncRNAs) have several important functions in the regulation of cell homeostasis and cell fate. Consequently, abnormal transcription of lncRNAs has been correlated with malignant transformation of cells. These human transcripts have been shown to participate in the progression of gastric cancer.

Methods: In the current project, we evaluated expression of a panel of lncRNAs including HULC, MALAT1, FAS-AS1, GAS5, PVT1, OIP5-AS1 and THRIL in 30 gastric cancer tissues and paired adjacent non-cancerous tissues (ANCTs) using quantitative real-time PCR.

Results: HULC, OIP5-AS1 and THRIL transcription quantities were significantly lower in gastric tumors compared to ANCTs (P values = .02, 0.02 and 0.007, respectively). Relative transcription quantities of HULC, MALAT1, OIP5-AS1, PVT1, FAS-AS1 and THRIL were associated with the site of the primary tumor (P values = .002, 0.003, 0.002, 0.002, 0.002, and 0.001, respectively). Moreover, relative expression levels of PVT1 were associated with history of smoking (P value = .04). Correlations were identified between transcript quantities of these lncRNAs in both tumor samples and ANCTs. Receiver operating characteristic curve assessment demonstrated that THRIL had the highest diagnostic power among the mentioned lncRNAs (area under curve (AUC) = 0.72, P value = .001). HULC and OIP5-AS1 ranked afterwards (AUC values of 0.69 and 0.68; P values = .005 and 0.007, respectively).

Conclusion: The current investigation underscores the dysregulation of these transcripts in gastric cancer specimens and suggests a number of these transcripts for further assessments of their suitability as cancer biomarkers.
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http://dx.doi.org/10.1016/j.yexmp.2020.104383DOI Listing
April 2020

Metastasis Associated Lung Adenocarcinoma Transcript 1: An update on expression pattern and functions in carcinogenesis.

Exp Mol Pathol 2020 02 9;112:104330. Epub 2019 Nov 9.

Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address:

The Metastasis Associated Lung Adenocarcinoma Transcript 1 (MALAT1) is among long non-coding RNAs (lncRNAs) which has disapproved the old term of "junk DNA" which was used for majority of human genome which are not transcribed to proteins. An extensive portion of literature points to the fundamental role of this lncRNA in tumorigenesis process of diverse cancers ranging from solid tumors to leukemia. Being firstly identified in lung cancer, it has prognostic and diagnostic values in several cancer types. Consistent with the proposed oncogenic roles for this lncRNA, most of studies have shown up-regulation of MALAT1 in malignant tissues compared with non-malignant/normal tissues of the same source. However, few studies have shown down-regulation of MALAT1 in breast cancer, endometrial cancer, colorectal cancer and glioma. In the current study, we have conducted a comprehensive literature search and provided an up-date on the role of MALAT1 in cancer biology. Our investigation underscores a potential role as a diagnostic/prognostic marker and a putative therapeutic target for MALAT1.
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http://dx.doi.org/10.1016/j.yexmp.2019.104330DOI Listing
February 2020

Curcumin loading potentiates the neuroprotective efficacy of FeO magnetic nanoparticles in cerebellum cells of schizophrenic rats.

Biomed Pharmacother 2018 Dec 2;108:1244-1252. Epub 2018 Oct 2.

Radiation Biology Research Center, Iran University of Medical Sciences, Tehran, Iran; Department of Medical Nanotechnology, Faculty of Advanced Technology in Medicine, Iran University of Medical Sciences, Tehran, Iran; Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran. Electronic address:

Background: The aim of this study was to investigate the neurotoxic effects of FeO magnetic- CurNPs on isolated schizophrenia mitochondria of rats as an in vivo model.

Methods: We designed CMN loaded superparamagnetic iron oxide nanoparticles (SPIONs) (FeO magnetic- CurNPs) to achieve an enhanced therapeutic effect. The physicochemical properties of FeO magnetic- CurNPs were characterized using X-ray diffraction (XRD), and dynamic laser light scattering (DLS) and zeta potential. Further, to prove FeO magnetic- CurNPs results in superior therapeutic effects, and also, the mitochondrial membrane potential collapse, mitochondrial complex II activity, reactive oxygen species generation, ATP level, cytochrome c release and histopathology of cerebellums were determined in brains of schizophrenic rats.

Results: We showed that effective treatment with CMN reduced or prevented FeO magnetic-induced oxidative stress and mitochondrial dysfunction in the rat brain probably, as well as mitochondrial complex II activity, MMP, and ATP level were remarkably reduced in the cerebellum mitochondria of treated group toward control (p < 0.05). Therewith, ROS generation, and cytochrome c release were notably (p < 0.05) increased in the cerebellum mitochondria of treated group compared with control group.

Conclusion: Taken together, FeO magnetic- CurNPs exhibits potent antineurotoxicity activity in cerebellums of schizophrenic rats. This approach can be extended to preclinical and clinical use and may have importance in schizophernia treatment in the future. To our knowledge this is the first report that provides the FeO magnetic- CurNPs could enhance the neuroprotective effects of CMN in the Schizophrenia.
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http://dx.doi.org/10.1016/j.biopha.2018.09.106DOI Listing
December 2018

Comparison of the effects of MnO-NPs and MnO-MPs on mitochondrial complexes in different organs.

Toxicol Mech Methods 2019 Feb 24;29(2):86-94. Epub 2018 Oct 24.

k Department of Pharmacology and Toxicology School of Pharmacy , Ardabil University of Medical Sciences , Ardabil , Iran.

Today, nanoparticles (NPs) have been widely used in various fields. Manganese oxide nanoparticles have attracted a lot of attention due to many applications. One of the major concerns regarding the widespread use of various NPs is the exposure and accumulation in human organs and finally toxicity. The generation of reactive oxygen species (ROS) by mitochondria is one of the most important mechanisms of toxicity suggested by published studies induced by other NPs. However, limited studies have been conducted on the mechanism of toxicity of MnO-NPs and MnO-microparticles (MnO-MPs). In this study, we compared the accumulation of MnO-NPs and MnO-MPs in different tissues and evaluated their effects on mitochondrial complexes in isolated mitochondria. Our results showed that intravascular (iv) administration of the MnO-NPs in the same dose compared to the MnO-MPs resulted in more accumulation in the C57 mouse female tissues. The effect of MnO-NPs and MnO-MPs in mitochondria showed that complexes I and III play an important role in increasing ROS generation and this effect is related to type of tissue. Also, our results showed that exposure to MnO-NPs and MnO-MPs reduced the activity of mitochondrial complexes II and IV. Our results suggest that the toxicity of the MnO-NPs is higher than that of the MnO-MPs and can lead to the depletion of antioxidant status, likely induction of apoptosis, cancer, and neurodegenerative disease. Abbreviations: NPs: nanoparticles; ROS: reactive oxygen species; SDH: succinate dehydrogenase; DCFH-DA: dichloro-dihydro-fluorescein diacetate; ELISA: enzyme-linked immunosorbent assay; MnO2-NPs: manganese oxide nanoparticles.
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http://dx.doi.org/10.1080/15376516.2018.1512693DOI Listing
February 2019

Protection of manganese oxide nanoparticles-induced liver and kidney damage by vitamin D.

Regul Toxicol Pharmacol 2018 Oct 10;98:240-244. Epub 2018 Aug 10.

Department of Pharmacology and Toxicology, School of Pharmacy, Ardabil University of Medical Sciences, Ardabil, Iran. Electronic address:

Metal nanoparticles (NPs) have been extensively used in industry as well as in biomedical application. Manganese oxide-nanoparticles (MnO-NPs) one of these materials, have many applications. This study was designed to evaluate the protective role of vitamin D against MnO-NPs -induced toxicity in the BALB c mice. These mice were randomly assigned to 4 (n = 10). In this study, MnO-NPs (10 mg/kg), vitamin D (10 mg/kg) and MnO-NPs plus vitamin D were administered interperitoneally once daily for 50 consecutive days. The liver and kidney functions, the levels of serum glucose, albumin (ALB), bilirubin (BIL) and total protein were studied. The results indicated that MnO-NPs administration significantly decreased liver and kidney functions, and increased glucose and bilirubin serum levels compared to control group (P < 0.05). However, vitamin D administration significantly boosted liver and kidney functions, decreased glucose and bilirubin serum level compared to the group received MnO-NPs (P < 0.05). It seems that vitamin D administration could protect the liver and kidney damage induced by MnO-NPs. Probably, given the use of these nanoparticles as a contest agent in humans, having normal levels of vitamin D or receiving it at the time of the test can inhibit liver and kidney toxicity induced by MnO-NPs.
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http://dx.doi.org/10.1016/j.yrtph.2018.08.005DOI Listing
October 2018

Nanocarriers Usage for Drug Delivery in Cancer Therapy.

Iran J Cancer Prev 2016 Apr 24;9(2):e3966. Epub 2016 Apr 24.

Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran.

Conventional therapeutic agents have displayed significant shortcomings. For this reason, important achievements have effectively made in biotechnology for delivering the therapeutic agents to the site of action, and diminish side effects. Polymeric carriers, micelles, dendrimers, liposomes, solid lipid carriers, gold carriers, viral carriers, nanotubes and magnetic carriers incorporating cytotoxic therapeutics have developed. To improve biological distribution of therapeutic drugs, some modified carriers have designed in optimal size and modified surface area. Delivery of carriers to target cells could be done by passive and active targeting.
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http://dx.doi.org/10.17795/ijcp-3966DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4951761PMC
April 2016

Factors associated with prostate cancer screening behavior among men over 50 in Fasa, Iran, based on the PRECEDE model.

Electron Physician 2015 Apr-Jun;7(2):1054-62. Epub 2015 Jun 5.

Cancer Prevention Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Background: Prostate cancer is one of the most common and lethal cancers in the world. The incidence of prostate cancer has been increasing in recent years. The purpose of this study was to investigate factors associated with prostate cancer screening behaviors among men over 50 in Fasa, Iran, based on the PRECEDE model.

Methods: In this cross-sectional study, 400 men over 50 were studied in Fasa, Iran. Data were collected via a questionnaire on demographic characteristics, such as age, number of children, occupation, education, marital status, smoking, and prostate cancer screening behaviors. Data were analyzed using SPSS software, version 16. Independent samples t-test and the Pearson Product Moment correlation coefficient were used for the statistical analyses.

Results: Men in the study had little knowledge (34.11±8.22) and attitude (28.23±7.23) about prostate cancer and screening behavior. Their mean scores about prostate cancer, screening behavior, quality of life, and general health were moderate. The subjects had low self-efficacy and perceived social support. Their mean scores of enabling factors and screening behaviors were at a low level. Pearson correlation scores showed a significant correlation between cancer prostate screening behavior and demographic variables, such as age (p=0.04, r=0.136), occupation (p=0.01, r=0.121), educational level (p=0.02, r=0.211), and marital status of the subjects (p=0.01, r=0.112), but there were not significant correlations with the number of children (p=0.12, r=0.092) and smoking (p=0.09, r=0.002). The T-test results showed significant relationships between age, occupation, and education of the subjects, and the PRECEDE model structures were significant for predisposing factors, enabling factors, and reinforcing factors (p<0.05).

Conclusion: The prostate cancer screening behaviors in men over 50 in Fasa, Iran, were at a low level. Due to predisposing factors, such as the knowledge, attitudes, and beliefs of individuals, reinforcing factors can have an important role in the behaviors related to prostate screening, such as their families and health staffs as well as enabling factors, such as health financing, access to medicines and learning self-care. Educational interventions to improve these behaviors and the quality of life of these people seem essential.
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http://dx.doi.org/10.14661/2015.1054-1062DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4477765PMC
June 2015

The effect of education on improvement of intake of fruits and vegetables aiming at preventing cardiovascular diseases.

Med J Islam Repub Iran 2015 18;29:183. Epub 2015 Feb 18.

MD, Researcher, Candidate for PhD by Research, Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Background: Cardiovascular diseases refer to a group of diseases that affect the cardiovascular system; principally cardiac diseases, vascular diseases of the brain and kidney and peripheral arterial diseases which are caused by various factors. Considering the importance of nutrition education, especially the intake of fruits and vegetables, this study was performed to determine the effect of health education, Based on the Health Belief Model, on the improvement of intake of fruits and vegetables aiming at preventing cardiovascular diseases among high school girls in the city of Shahre- Kord, Iran.

Methods: This was a quasi-experimental intervention study, in which 120 female students of high schools in Isfahan were selected through convenient sampling and were divided into two groups of experimental (60) and control (60). The instruments for data collection were the Health Belief Model and FFQ questionnaires. The HBM questionnaire was completed three times (before, immediately and two months after the intervention) and the FFQ questionnaire was completed two times (before and two months after the intervention) by the students. After the pre-test, six educational sessions were provided for the experimental group. Finally, data were collected and analyzed by SPSS 16 (ttest, paired t-test and repeated measure ANOVA).

Results: There were no differences between the two groups in terms of demographic variables. Before the intervention, there were not any significant differences between the scores of different structures of this model between the two groups (p>0.05); however, after the intervention, significant differences were found between the experimental and control groups in the levels of knowledge, perceived susceptibility, perceived severity, perceived benefits, perceived barriers, perceived efficacy and performance (p<0.001).

Conclusion: According to the results, the intervention had a positive impact on the improvement of intake of fruits and vegetables among the students.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4431428PMC
June 2015

Analytical assessment of interleukin - 23 and -27 cytokines in healthy people and patients with hepatitis C virus infection (genotypes 1 and 3a).

Hepat Mon 2014 Sep 27;14(9):e21000. Epub 2014 Sep 27.

Department of Social Medicine, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, IR Iran ; Young Researchers Club, Shahr-e-Qods branch, Islamic Azad University, Tehran, IR Iran.

Background: The immune system plays important roles in determining the outcomes of hepatitis C virus (HCV) infection. Interleukin-23 and -27 (IL-23 and IL-27) are two novel IL-12 cytokine family members known to enhance the T-lymphocyte response, but their precise involvement in HCV infection is not well known.

Objectives: We investigated the serum IL-27 and IL-23 levels in patients with HCV infection and in healthy individuals.

Patients And Methods: In this case-control study, we assessed IL-23 and IL-27 levels in serum of 37 healthy individuals and 64 patients with chronic HCV using Enzyme-linked immunosorbent assay (ELISA). The relationship of cytokines level with liver enzymes (ALT, AST, and ALP), HCV genotype and viral load were analyzed. The differences of these cytokine levels in the groups of treatment and no treatment was compared. HCV genotypes were classified by HCV-specific primers methods. HCV RNA loads were determined by fluorescence quantitative PCR.

Results: Serum level of IL-23 was higher in HCV infected patients compared to control group (P = 0.005). However, no significant difference was seen in IL-27 serum level between patients compared to the control group (P = 0.65). There was no significant difference in IL-23 and IL-27 level between genotype 1 HCV-infected- and 3a HCV-infected- patients. Positive moderate correlation between IL-23 and IL-27 with viral load was found in type 3a and 1 HCV-infected patient. Positive relative correlation was seen between ALT and IL-23 in 1a HCV-infected patients, which was higher than 3a HCV-infected patients; but there were no significant difference between serums liver enzymes with IL-23 and IL-27 in respect to genotype 3a and 1a HCV-infected patients.

Conclusions: These findings may reflect a vigorous pro-inflammatory reaction orchestrated by the host immune system against chronic HCV. Also, a better understanding of the involvement mechanism considering the correlation between other genotypes with inflammatory cytokines in various stages of disease can be obtained.
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http://dx.doi.org/10.5812/hepatmon.21000DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4221962PMC
September 2014

Molecular epidemiology of different hepatitis C genotypes in serum and peripheral blood mononuclear cells in jahrom city of iran.

Hepat Mon 2014 May 11;14(5):e16391. Epub 2014 May 11.

Department of Medical Lab Technology and Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran.

Background: The Hepatitis C Virus (HCV) is considered essentially hepatotropic, yet the virus compartments have also been found in important extra hepatic sites. Detection of HCV RNA in extra hepatic reservoirs such as peripheral blood mononuclear cells (PBMCs) is important for determining disease progression and treatment effectiveness.

Objectives: The present study aimed to determine different HCV genotypes in patients' plasma and PBMC specimens, in Jahrom city of Iran.

Patients And Methods: Blood samples of 137 patients with established HCV were collected at the Honari clinic. These patients were anti-HCV and plasma HCV RNA positive. After plasma RNA extraction and obtaining a pellet of approximately 3-5 × 10(6) PBMCs, Real-time PCR was performed, using specific-genotype primers. Finally, data analysis was done by the Statistical Package for Social Sciences (SPSS) software.

Results: Subtype 3 was the most common genotype in plasma (57.7%) and PBMCs (51.1%). Subtype 1a was detected in 36.5% and 30.7% of plasma samples and PBMCs, respectively whereas subtype 4 was not detected in any of the cases. There was a genotype difference between plasma and PBMCs of 12.4% of patients. In four patients no genotype was detected in their plasma but genotype 3 was detected in the PBMCs.

Conclusions: It is suggested that determination of the target genotype by plasma subtyping for choosing the proper antiviral therapy is essential but may result in therapy failure. HCV genotyping in PBMC samples, along with plasma specimens, might be more beneficial. Therefore determining the HCV genotype in PBMCs, before beginning the therapy is useful due to the possibility of occult infection detection.
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http://dx.doi.org/10.5812/hepatmon.16391DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4071316PMC
May 2014

The effect of education on the improvement of fruits and vegetables consumption aiming to preventing colorectal cancer.

Gastroenterol Hepatol Bed Bench 2014 ;7(2):94-100

Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran ; Medical Education Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

Aim: This study was performed to determine the effect of health education on the improvement of intake of fruits and vegetables aiming at preventing colorectal cancer among high school girls in the city of Shahr-e-kord.

Background: Colorectal cancer is one of the most important and most common cancers and the second leading cause of cancer death worldwide. Every year, nearly one million new cases of colorectal cancer are recognized around the world and nearly half of them lose their lives due to the disease.

Patients And Methods: 130 students were randomly divided to two groups, which experimental (65) and controls (65) were chosen from the city of Shahr-e-kord for this experimental study. The instruments for data collecting were self-made questionnaire health belief model based and food frequency questionnaire. The HBM FFQ questionnaires were completed before, immediately and two months after education by participants. After pre-test, 5 educational session classes in experimental group were performed. Finally, data collected and analyzed by SPSS16 computer software (T- test, T-Paired, repeated measure ANOVA).

Results: Findings of the study showed that before the intervention there was no significant differences between the scores of different structures of HBM model in two groups (P > 0.05), after the intervention there was significant differences between experimental and control groups in the levels of knowledge, structures of HBM model and performance for preventing colorectal cancer (p < 0.001).

Conclusion: Performing educational programs based on health belief model increases the knowledge and improves the attitudes and practices of students regarding prevention of the colorectal cancer.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4017560PMC
May 2014

Quality of life in peptic ulcer patients referring to Al-Zahra hospital of Isfahan, Iran.

Gastroenterol Hepatol Bed Bench 2013 ;6(Suppl 1):S87-92

Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.

Aim: The purpose of this study was to determine quality of life in peptic ulcer patients referring to Al-Zahra hospital of Isfahan.

Background: Peptic ulcer disease (PUD) is one of the most prevalent diseases. Its prevalence is 6-15% and about 10% of people experience its symptom in their life. PUD can have a considerable impact on patients' quality of life (QOL).

Patients And Methods: This descriptive- analytic survey was done on 93 randomly patients referred to Al-Zahra hospital of Isfahan city in Iran. Data gathering was done via questionnaire including five domains: physical, psychological, social, behavioral and economical. For data analysis, t-test, Pearson correlation and ANOVA test were used.

Results: 93 patients with mean age of 38.54 years, including 43 (46.2%) women and 54 (53.8%) men, were studied. There was a negative significant between quality of life and age and between disease duration and psychological, economical domains and between the mean of QOL scores in physical and social domains with the number of cigarette per day, also there was significant relation between social domain and gender, and physical, psychological and behavioral domains with marital status; Physical, social domains with smoking. Also there was a negative significant between physical, social and behavioral domains with years of smoking.

Conclusion: Study results showed that quality of life is in a relatively good level among patients, thus some diseases such as peptic ulcer can effect on quality of life. So, treatment and prevention of these diseases may improve their quality of life.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4017537PMC
May 2014