Publications by authors named "Arushi Verma"

17 Publications

  • Page 1 of 1

Sotos syndrome with a novel mutation in the gene associated with congenital hypothyroidism.

Int J Pediatr Adolesc Med 2021 Sep 26;8(3):191-194. Epub 2020 Jun 26.

Division of Endocrinology and Diabetes, Seattle Children's Hospital/University of Washington, Seattle, WA, 98105, USA.

Childhood overgrowth syndromes are relatively rare. A generalized overgrowth syndrome should be suspected when tall stature and macrocephaly are present, after ruling out nutritional excess and endocrinopathies. Sotos syndrome is a well-described overgrowth syndrome due to haploinsufficiency of the gene. We present a case of an infant with permanent congenital hypothyroidism, who had tall stature and macrocephaly by 7 months of age. He was noted to have typical facial features, mild gross motor and speech delay, and scoliosis by 13 months of age. Gene sequencing revealed a heterozygous novel c6076_6087del12: p.Asn2026_Thr2029del variant in exon 20 of the gene, pathogenic for Sotos syndrome. Congenital hypothyroidism with Sotos syndrome has been infrequently reported and may expand the spectrum of disease characteristics. Early diagnosis of overgrowth syndromes is important for developmental follow up and multidisciplinary care coordination.
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http://dx.doi.org/10.1016/j.ijpam.2020.06.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8319649PMC
September 2021

Recurrent Vomiting in a 6-year-old Boy.

Pediatr Rev 2021 08;42(8):453-456

Department of Pediatrics, University of Washington, Seattle, WA.

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http://dx.doi.org/10.1542/pir.2020-001024DOI Listing
August 2021

Autonomous magnetic microbots for environmental remediation developed by organic waste derived carbon dots.

J Environ Manage 2021 Nov 26;297:113322. Epub 2021 Jul 26.

Department of Applied Sciences, Indian Institute of Information Technology Allahabad, Prayagraj, UP, India. Electronic address:

Biodegradable precursors for micro/nanobots development are key requirements for several sustainable applications. In this regard, we propose an innovative solution for water purification at minimum cost and efforts where organic waste is used for the treatment of organic pollutants. Herein, catalytic magnetic microbots were developed by functionalizing iron oxide nanoparticles with carbon dots (C-Dots), which were synthesized by using household waste such as potato peels as precursors. The speed of these autonomously propelling bots indeed is found very promising for large distance swimming even in viscous medium by using hydrogen peroxide as fuel. These microbots catalytically propel and degrade toxic polar as well as sparingly water-soluble industrial dyes without any external agitation. The degradation of dyes was confirmed by mass-spectra analysis. Furthermore, these microbots can efficiently degrade a mixture of dyes and reused without compromising its performance significantly. Additionally, rate constant (K) and activation energy (E) were also determined to establish the catalytic nature of the bots. The present microbots acted as nanozyme owing to its synergistic catalytic activity of FeO and C-Dots for degradation of mixture of toxic dyes, essential for large scale water treatment.
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http://dx.doi.org/10.1016/j.jenvman.2021.113322DOI Listing
November 2021

Corrigendum to "Green synthesis of metallic nanoparticles as effective alternatives to treat antibiotics resistant bacterial infections: A review" [Biotechnol. Rep. 25 (2020) e00427].

Biotechnol Rep (Amst) 2021 Mar 19;29:e00578. Epub 2021 Mar 19.

Department of Applied Sciences, Indian Institute of Information Technology Allahabad, Allahabad, 211012, India.

[This corrects the article DOI: 10.1016/j.btre.2020.e00427.].
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http://dx.doi.org/10.1016/j.btre.2020.e00578DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7990725PMC
March 2021

Combination therapy of biogenic C-dots and lysozyme for enhanced antibacterial and antibiofilm activity.

Nanotechnology 2021 Feb;32(8):085104

Department of Applied Sciences, Indian Institute of Information Technology Allahabad, Allahabad-211012, Uttar Pradesh, India.

Nearly 80% of human chronic infections are caused due to bacterial biofilm formation. The increased resistance against the conventional antimicrobial agents makes it difficult to treat the biofilm-related infections. The antibiotics resistance developed by planktonic cells has also become a major threat for human. Therefore, we have attempted here to develop an effective alternative strategy to overcome the issues of antibiotics resistance of bacteria. Upon synthesis, biogenic C-dots were combined with lysozymes which were further encapsulated into chitosan nanocarrier to form C-dots carrier (CDC). The as-synthesized C-dots were found irregular shaped and the average size of C-dots and CDC were 8 ± 2 nm and 450 ± 50 nm, respectively. To ensure secure and targeted delivery of C-dots and lysozyme we have employed chitosan, a biodegradable and natural biopolymer, as a delivery system. The study of time-dependent bacterial growth and flow cytometry analysis demonstrated that CDC can exhibit a synergistic bactericidal activity against the antibiotics resistant recombinant E. coli cells. Further, we have shown that the CDC could be a potent agent for both prevention of biofilm formation and eradication of preformed biofilm. In addition, we have observed that our drug delivery system is hemocompatible in nature making it suitable for in vivo applications. Therefore, we believe that the combination therapy of C-dots and lysozyme may be used as an excellent antibacterial and antibiofilm strategy.
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http://dx.doi.org/10.1088/1361-6528/abc2edDOI Listing
February 2021

Nanocomposite of Ag nanoparticles and catalytic fluorescent carbon dots for synergistic bactericidal activity through enhanced reactive oxygen species generation.

Nanotechnology 2020 Oct 4;31(40):405704. Epub 2020 Jun 4.

Department of Applied Sciences, Indian Institute of Information Technology Allahabad, Allahabad 211012, Uttar Pradesh, India.

Microwave mediated synthesis of catalytic fluorescent carbon dots (Cdots) has been reported using biodegradable starch as precursor. The as-synthesized Cdots were then characterized using various techniques such as fluorescence spectroscopy, fourier-transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM) and x-ray photoelectron spectroscopy (XPS) analysis. Interestingly, Cdots showed high catalytic activity in the photo-reduction of Ag to silver nanoparticles (Ag NPs). During the photo-reduction process, no additional surface passivating agents was needed to stabilize the Ag NPs. Further, TEM results indicated the formation of Cdot-Ag NP nanocomposite i.e. Ag NPs surrounded with Cdots, and the emission intensity of Cdots was significantly decreased whereas the lifetime of Cdots remained almost unaltered in the presence of Ag NPs following static quenching. Finally, combination therapy of Cdots and Ag NPs using Cdot-Ag NP nanocomposite was performed which indicated synergistic bactericidal activity against antibiotic resistant recombinant E. coli bacteria. The treatment elevated the reactive oxygen species (ROS) level as compared to its individual components. Additionally, the flow cytometer study demonstrated that combination therapy causing bacterial cell wall perforation that was possibly leading to synergistic bactericidal activity against both Gram positive and Gram negative bacteria. The presence of Cdots on the surface of the Ag NPs due to their ground state complexation, possibly facilitated electrons towards Ag NPs which enhanced the ROS production in comparison to only Ag NPs.
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http://dx.doi.org/10.1088/1361-6528/ab996fDOI Listing
October 2020

Green synthesis of metallic nanoparticles as effective alternatives to treat antibiotics resistant bacterial infections: A review.

Biotechnol Rep (Amst) 2020 Mar 31;25:e00427. Epub 2020 Jan 31.

Department of Applied Sciences, Indian Institute of Information Technology Allahabad, Allahabad, 211012, India.

Due to development of bacterial resistance to the conventional antibiotics, the treatment of bacterial infections has become a major issue of concern. The unprescribed and uncontrolled use of antibiotics has lead to the rapid development of antibiotic resistance in bacterial strains. Therefore, the development of novel and potent bactericidal agents is of great clinical importance. Interestingly, metallic nanoparticles (NPs) have been proven to be promising alternative to antibiotics. NPs interact with the important cellular organelles and biomolecules like DNA, enzymes, ribosomes, and lysosomes that can affect cell membrane permeability, oxidative stress, gene expression, protein activation, and enzyme activation. Since, NPs target multiple biomolecules concurrently; it becomes very difficult for bacteria to develop resistance against them. Currently, there are different physical and chemical methods utilized for NPs synthesis. However, most of these processes are costly and potentially hazardous for the living organisms and environment. Therefore, there is a need to develop an eco-friendly and cost-effective method of synthesis. Recently, the 'green synthesis' approaches are gaining a lot of attention. It is demonstrated that living organisms like bacteria, yeast, fungi, and plant cells can reduce inorganic metal ions into metal NPs by their cellular metabolites. Both the yield and stability of biogenic NPs are quite satisfactory. In the current article, we have addressed the green synthesis of various metal NPs reported till date and highlighted their different modes and mechanisms of antibacterial properties. It is highly anticipated that biogenic metallic NPs could be viable and economical alternatives for treating drug resistant bacterial infections in near future.
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http://dx.doi.org/10.1016/j.btre.2020.e00427DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7005563PMC
March 2020

Role of surface charge in enhancing antibacterial activity of fluorescent carbon dots.

Nanotechnology 2020 Feb 8;31(9):095101. Epub 2019 Nov 8.

Department of Applied Sciences, Indian Institute of Information Technology Allahabad, Allahabad 211012, Uttar Pradesh, India.

Herein, different surface charged carbon dots (Cdots) were synthesized by using diethylene glycol as a carbon source with various amine containing surface passivating agents. The synthesis method is very simple and fast microwave oven-based, that results in almost similar sized positive, negative and uncharged fluorescent Cdots which has been confirmed by zeta potential analysis in our case. The formation of Cdots was confirmed by characterization using fluorescence spectroscopy, transmission electron microscopy, XRD, FT-IR, and XPS spectroscopy. To find out relative bactericidal activity of these Cdots, green fluorescence protein expressing recombinant E. coli bacteria were taken as a model system. Time-dependent bacterial growth and FACS study demonstrated that both uncharged Cdots and positively charged Cdots were showing better bactericidal activity as compared to negative charged Cdots. The Cdots caused elevation of reactive oxygen species level, which is possibly leading to bacterial cell death.
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http://dx.doi.org/10.1088/1361-6528/ab55b8DOI Listing
February 2020

Multiple Abusive Fractures in an Infant With a Concurrent Parathyroid Hormone-Related Peptide-Secreting Renal Tumor: Abusive Fractures Accompanying a Parathyroid Hormone-Related Peptide-Secreting Tumor.

Pediatr Emerg Care 2021 Jun;37(6):e339-e341

From the Multicare, Spokane.

Background: When evaluating an infant with unexplained fractures for child abuse, it is important to evaluate for possible causes of underlying bone fragility.

Case: A 7-month-old infant was found to have a parathyroid hormone (PTH)-related peptide-secreting mesoblastic nephroma. In spite of having an elevated serum calcium, depressed serum phosphate, and high levels of PTH-related peptide, he had no demineralization or other hyper parathyroid-related bone changes. Instead, he had multiple classic metaphyseal lesions, fractures of differing ages including a proximal clavicle fracture, and current and past bruising. No fractures typical of bone insufficiency were present. These findings are highly indicative of abuse in addition to his hormone-secreting tumor.

Conclusions: In spite of this child's abuse findings, endogenous or tumor-related hyper PTH should be in the differential of underlying bone fragility. Children with disorders that could cause injury susceptibility can also be abused.
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http://dx.doi.org/10.1097/PEC.0000000000001796DOI Listing
June 2021

The Impact of "Mom/Dad of the Day" Cards, Newborn Heart Auscultation, and Father Skin-to-Skin Care on Parent-Newborn Bonding.

Clin Pediatr (Phila) 2019 03 10;58(3):349-353. Epub 2018 Dec 10.

1 Texas Tech University Health Sciences Center, Amarillo, TX, USA.

Texas Tech Pediatrics at Northwest Texas Hospital System in Amarillo, Texas, currently practices giving "Mom/Dad of the Day" cards to all new parents, offering the opportunity for fathers to auscultate the newborn heart sound, and encouraging maternal and paternal skin-to-skin contact. New parents were asked to fill out 2 anonymous surveys regarding these practices. Survey results showed statistically significant positive responses by the parents for subjective improvement in fetal sleep and feeding as well as parental confidence and preparedness for taking care of their newborn. Additionally, these practices are desired by parents, with 96% fathers recommending that the hospital routinely offering opportunities for heart auscultation and 94% parents recommending "Mom/Dad of the Day" cards for all future parents in the hospital. Small, nonmedical initiatives such as these are well received and can be feasibly adopted in more hospitals to improve patient quality of care.
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http://dx.doi.org/10.1177/0009922818817312DOI Listing
March 2019

Family-Centered Care to Complement Care of Sick Newborns: A Randomized Controlled Trial.

Indian Pediatr 2017 Jun;54(6):455-459

Departments of Pediatrics and $Microbiology, PGIMER and Dr. RML Hospital; *Biostatistics, AIIMS; and #Management Studies, IIT; New Delhi, India. Correspondence to: Dr Arti Maria, Neonatal Division, Department of Pediatrics, PGIMER and Dr. RML Hospital, Baba Kharak Singh Marg, New Delhi, India 110 001.

Objective: To assess the impact of family-centered care in delivery of care to sick newborns, on nosocomial infection rate.

Design: Randomized controlled trial.

Setting: Tertiary referral nursery (October 2010 to March 2012).

Participants: 295 neonates randomized at the time of hospitalization in neonatal intensive care unit.

Intervention: Parent-attendant of intervention group were trained using an indigenously developed and pretested, culturally sensitive, simple audio-video tool that covered domains of personal hygiene, hand washing, danger signs recognition and feeding of sick neonate. Control group received routine care by nurses and doctors.

Outcome Measure: Primary: culture positive nosocomial infection rate. Secondary: culture negative nosocomial infection rate, duration of hospitalization, mortality and breastfeeding rate.

Results: Two-thirds of family caregivers were fathers/ mothers and about 20% were grandparents. About 60% of family care givers were either illiterate (25%) or primary/middle pass (34%). Incidence of nosocomial episodes of sepsis was not different between groups (incidence rate difference 0.74, 95 % CI -4.21, 5.6, P = 0.76). Pre-discharge exclusive breastfeeding rates were significantly higher in intervention group [80.4% vs 66.7% (P=0.007)].

Conclusion: There was no significant difference in nosocomial infection rate between the two groups. Translating and adapting principles of family-centered care was feasible, and improved the pre-discharge exclusive breastfeeding rates.
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http://dx.doi.org/10.1007/s13312-017-1047-9DOI Listing
June 2017

Urinary biomarkers as indicator of chronic inflammation and endothelial dysfunction in obese adolescents.

BMC Obes 2017 22;4:11. Epub 2017 Mar 22.

Department of Pediatrics, Texas Tech University Health Sciences Center, 1400 S. Coulter, Amarillo, TX 79106 USA.

Background: Obesity is a pro-inflammatory state that may predispose patients to acute coronary syndrome characterized by chronic low grade inflammation resulting in endothelial dysfunction (ED). The aim of the study was to evaluate urinary biomarkers of inflammation and ED in adolescents with obesity.

Methods: Sixty three subjects were recruited for the study. Twenty healthy adolescents with normal body mass (NW), 14 overweight (OW), 29 obese (OA) subjects were selected. An EndoPat 2000 device was used to measure the reactive hyperemia index (RHI). First morning fasting urine samples were tested for interleukin 6 (IL-6), endothelin 1 (ET-1), alpha-1-acid glycoprotein (AGP), tumor necrosis factor- α (TNF-α) and corrected to urinary creatinine.

Results: Urinary TNF-α was significantly higher in OA group (52.4 ± 15.3 pg/mg) compared to adolescents with NW (14.1 ± 1.2 pg/mg,  = 0.04). ET-1 levels were found to be higher in OW (5.18 ± 1.6 pg/mg) compared with NW (3 · 47 ± 0.3 pg/mg,  = 0.24); and higher in OA (8.48 ± 3.1 pg/mg) compared to both NW ( = 0.19) and OW ( = 0.40). Similarly a higher AGP level was observed in OW (864.8 ± 156 ng/mg) and OA (808.3 ± 186 ng/mg) compared to NW (653 ± 69 ng/mg) ( = 0.16 & 0.49 respectively). Inflammatory markers namely, TNF-α, IL-6 and AGP significantly and positively correlated with each other and with ET-1, a marker for endothelial dysfunction. This significant correlation was also observed when tested separately in the subgroups (NW, OW and OA). There were no differences in RHI levels among the study groups.

Conclusion: Urinary TNF-alpha is significantly elevated in obese adolescents and correlates with urinary ET-1, which is recognized as a biomarker for endothelial dysfunction. Since obesity is a chronic inflammatory state, elevated urinary TNF-alpha might be used as a non invasive tool to monitor the level of that inflammation.
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http://dx.doi.org/10.1186/s40608-017-0148-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5361775PMC
March 2017

Diagnostic paradox: Ruptured aneurysm of sinus of Valsalva simulating tricuspid valve endocarditis.

Echocardiography 2017 Mar 18;34(3):465-467. Epub 2017 Feb 18.

Department of Pediatrics, Texas Tech University Health Science Center, Amarillo, TX, USA.

Rupture of aneurysm of sinus of Valsalva into the right atrium mimicking tricuspid valve endocarditis is a rare presentation. We review a case of spontaneous rupture of aneurysm of sinus of Valsalva into the right atrium presenting as a murmur. Transthoracic echocardiogram showed a mobile mass that appeared to be attached to the tricuspid valve leaflet with moderate tricuspid regurgitation suggestive of tricuspid valve endocarditis. The diagnosis was confirmed as spontaneous rupture of noncoronary sinus in to the right atrium by transesophageal echocardiogram. Patient recovered completely after surgical repair.
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http://dx.doi.org/10.1111/echo.13479DOI Listing
March 2017

An unusual association of microcephalic osteodysplastic primordial dwarfism type I with cardiac and brain anomalies.

J Clin Neonatol 2014 Jan;3(1):53-4

Department of Pediatrics, PGIMER and Dr. Ram Manohar Lohia Hospital, New Delhi, India.

Less than 100 cases of primordial dwarfism have been reported worldwide out of which Microcephalic osteodysplastic primordial dwarfism type I comprise about <30 cases. We report a rare case of extreme growth failure in a neonate with primordial dwarfism of antenatal onset due to Microcephalic osteodysplastic primordial dwarfism type I. Our case is also unique in being associated with hitertho unreported association of subpulmonic ventricular septal defect and a dorsal interhemispheric cyst in the brain.
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http://dx.doi.org/10.4103/2249-4847.128743DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3982344PMC
January 2014

Lethal progressive thoracic insufficiency in a neonate due to jarcho levin syndrome.

J Clin Neonatol 2014 Jan;3(1):49-50

Department of Pediatrics, Medanta Medicity Hospital, New Delhi, India.

A rare case of Jarcho Levin syndrome (JLS) presenting as a lethal progressive respiratory insufficiency in early neonatal period is reported. The neonate had classical features of this syndrome including vertebral segmentation defects, typical costo-vertebral fusion defects and scoliosis resulting in small thoracic volume and limited chest expansion; all consistent with a clinical diagnosis of JLS with thoracic insufficiency. In addition, our case had a rare association of dextrocardia and acyanotic congenital heart disease.
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http://dx.doi.org/10.4103/2249-4847.128739DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3982342PMC
January 2014

Intractable diarrhoea caused by cytomegalovirus enterocolitis in an immunocompetent term neonate.

J Trop Pediatr 2013 Dec 18;59(6):509-11. Epub 2013 Jun 18.

Department of Pediatrics, PGIMER & Dr RML Hospital, New Delhi 110001, India.

Symptomatic cytomegalovirus (CMV) infection mainly affects preterm and immunocompromised infants and usually manifest as rash, pneumonia, hepatospleenomegaly or encephalitis. To our knowledge intractable diarrhoea at two weeks of age caused by postnatally acquired CMV in immunocompetent term neonate is not reported. An unusual case of postnatally acquired CMV enterocolitis manifesting as protracted diarrhoea in an immunocompetent baby in neonatal period is reported. We conclude that CMV should be considered in the differential diagnosis of intractable diarrhoea in neonatal period and treatment with intravenous ganciclovir for CMV enterocolitis is not only indicated but is therapeutic.
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http://dx.doi.org/10.1093/tropej/fmt052DOI Listing
December 2013
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