Publications by authors named "Artur Owczarek"

8 Publications

  • Page 1 of 1

Potential of Bacterial Cellulose Chemisorbed with Anti-Metabolites, 3-Bromopyruvate or Sertraline, to Fight against Lawn Biofilm.

Int J Mol Sci 2020 Dec 14;21(24). Epub 2020 Dec 14.

Department of Pharmaceutical Microbiology and Parasitology, Wroclaw Medical University, 50-556 Wroclaw, Poland.

is a bacterium known mainly of its ability to cause persistent inflammations of the human stomach, resulting in peptic ulcer diseases and gastric cancers. Continuous exposure of this bacterium to antibiotics has resulted in high detection of multidrug-resistant strains and difficulties in obtaining a therapeutic effect. The purpose of the present study was to determine the usability of bacterial cellulose (BC) chemisorbed with 3-bromopyruvate (3-BP) or sertraline (SER) to act against lawn biofilms. The characterization of BC carriers was made using a N2 adsorption/desorption analysis, tensile strength test, and scanning electron microscopy (SEM) observations. Determination of an antimicrobial activity was performed using a modified disk-diffusion method and a self-designed method of testing antibacterial activity against biofilm microbial forms. In addition, bacterial morphology was checked by SEM. It was found that BC disks were characterized by a high cross-linking and shear/stretch resistance. Growth inhibition zones for BC disks chemisorbed with 2 mg of SER or 3-BP were equal to 26.5-27.5 mm and 27-30 mm, respectively. The viability of lawn biofilm cells after a 4-h incubation with 2 mg SER or 3-BP chemisorbed on BC disks was ≥4 log lower, suggesting their antibacterial effect. SEM observations showed a number of morphostructural changes in cells exposed to these substances. Concluding, SER and 3-BP chemisorbed on BC carriers presented a promising antibacterial activity against biofilm cells in in vitro conditions.
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http://dx.doi.org/10.3390/ijms21249507DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7765062PMC
December 2020

Application of bacterial cellulose experimental dressings saturated with gentamycin for management of bone biofilm in vitro and ex vivo.

J Biomed Mater Res B Appl Biomater 2020 01 18;108(1):30-37. Epub 2019 Mar 18.

Department of Immunology, Microbiology and Physiological Chemistry, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology in Szczecin, 70-311, Szczecin, Poland.

Bacterial cellulose is one of the most promising polymers of recent years. Herein, we present a possibility of BC application as a carrier of gentamycin antibiotic for the treatment and prevention of bone infections. We have shown that BC saturated with gentamycin significantly reduces the level of biofilm-forming bone pathogens, namely Staphylococcus aureus and Pseudomonas aeruginosa, and displays very low cytotoxicity in vitro against osteoblast cell cultures. Another beneficial feature of our prototype dressing is prolonged release of gentamycin, which provides efficient protection from microbial contamination and subsequent infection. Moreover, it seems that bacterial cellulose (BC) alone without any antimicrobial added, may serve as a barrier by significantly hampering the ability of the pathogen to penetrate to the bone structure. Therefore, a gentamycin-saturated BC dressing may be considered as a possible alternative for gentamycin collagen sponge broadly used in clinical setting. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 108B:30-37, 2020.
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http://dx.doi.org/10.1002/jbm.b.34362DOI Listing
January 2020

Development of the rectal dosage form with silver-coated glass beads for local-action applications in lower sections of the gastrointestinal tract.

Pharm Dev Technol 2018 Mar 3;23(3):295-300. Epub 2017 Aug 3.

c Department of Drug Form Technology, Faculty of Pharmacy , Wroclaw Medical University , Wroclaw , Poland.

Context: Recent findings indicating the anti-inflammatory action of silver preparations through modulation of the gut microbiota and apoptosis of inflammatory cells predestine silver use in inflammatory bowel disease (IBD).

Objective: The aim of our study was to validate the possibility of effective silver release from silver-coated glass beads for anti-inflammatory local application in the lower sections of the gastrointestinal (GI) tract.

Materials And Methods: Silver-coated glass beads were prepared using magnetron method. Release of silver from the silver-coated glass bead surface was carried out in BIO-DIS reciprocating cylinder apparatus. Erosion of silver coating and indirect estimation of the silver release dynamics was assessed using scanning electron microscope. Rectal suppositories containing silver-coated glass beads were prepared using five different methods (M1-M5) and X-ray scanned for their composition.

Results And Discussion: The XR microanalysis and the chemical composition analysis evidenced for a rapid (within 30 min) release of nearly 50% of silver from the coating of the glass beads, which remained stable up to 24 h of incubation. The most homogeneous distribution of beads in the entire volume of the suppository was obtained for formulation M5, where the molten base was poured into mold placed in an ice bath, and the beads were added after 10 s.

Conclusions: Our study is the first to present the concept of enclosing silver-coated glass beads in the lipophilic suppository base to attenuate inflammation in the lower GI tract and promises efficient treatment with reduced side effects.
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http://dx.doi.org/10.1080/10837450.2017.1359843DOI Listing
March 2018

Thermal stability and decompositions kinetics under non-isothermal conditions of imatinib mesylate α form.

J Pharm Biomed Anal 2016 Sep 21;129:9-14. Epub 2016 Jun 21.

Department of Drug Form Technology, Wroclaw Medical University, Borowska 211 A, 50-556 Wroclaw, Poland.

The thermal decomposition and kinetic parameters of synthetized imatinib mesylate α form α form were determined by thermogravimetry (TGA/DTG) under non-isothermal conditions. The experiments were performed at a 25-940°C temperature range at five different heating rates: 2.5Kmin(-1), 5Kmin(-1), 10Kmin(-1), 15Kmin(-1) and 20Kmin(-1) per minute in a nitrogen atmosphere. Imatinib mesylate α form presents one-step mass loss during the degradation process. The thermal stability of the examined material, the melting temperature (Tonset=220.6°C) and ΔH fusion=-95.74Jg(-1) at a heating rate of 10°Cmin(-1) was established. The values of activation energies have been estimated using Kissinger, Flynn-Wall-Ozawa (FWO) and Kissinger-Akahira-Sunose (KAS) methods.
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http://dx.doi.org/10.1016/j.jpba.2016.06.032DOI Listing
September 2016

Solid dispersion in pharmaceutical technology. Part II. The methods of analysis of solid dispersions and examples of their application.

Polim Med 2012 ;42(2):97-107

Department of Drug Form Technology, Wroclaw Medical University, Wroclaw, Poland.

In the first part of the article solid dispersions were classified the properties and methods of their preparation were described. This section presents methods of analysis of solid dispersions i.e.: thermoanalytical methods, XRPD, FTIR, microscopic methods, dissolution studies and examples of drug forms where solid dispersions were used.
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October 2012

Solid dispersions in pharmaceutical technology. Part I. Classification and methods to obtain solid dispersions.

Polim Med 2012 ;42(1):17-27

Department of Drug Form Technology, Wroclaw Medical University, ul. Szewska 38, Wroclaw, Poland.

There are many methods to increase solubility of a substance. These include, inter alia, preparation of solid dispersions, i.e. eutectic mixtures, solid solutions, glassy solutions and suspensions. When compared to the individual constituents prior to dispersion formation solid dispersion components are better soluble in water. Therefore, solid solutions became one of the most promising ways to modify solubility, ensuring improved bioavailability and consequently therapeutic efficacy of a substance. In this part of the publication solid dispersions were classified and described in regard to their properties and preparation methods, i.e. melting method, melt evaporation and melt extrusion methods, lyophilisation technique, melt agglomeration process as well as SCF technology and electrospinning.
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August 2012

[In vitro evaluation of the gels properties prepared thermosensitive polymers as vehicles for administration substance by injection].

Polim Med 2011 ;41(4):3-15

Katedra i Zakład Technologii Postaci Leku, Akademia Medyczna we Wrocławiu.

The aim of this study was to prepare a thermoresponsive formulations, which are a carrier for substance administered directly into site of action and which obtain sol-gel transitions at physiological ranges of temperature. The investigated formulations of liquid consistency at room temperature were obtained in sterile conditions on the basis of nonionic polymers Pluronic F-127, Pluronic F-68 and anionic polymer hyaluronic acid in different concentrations. The sol-gel transition temperature of the formulations was investigated and their physicochemical properties such as pH, density, osmotic pressure, sol-gel transition temperature, texture and release of vancomycin hydrochloride were studied. In vitro release experiments indicated that the optimised platform was able to prolong vancomycin hydrochloride release and their physico-chemical properties allow for application by injection form.
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March 2012

[Evaluation properties of hydrogels with simvastatin prepared on Carbopol 2020].

Polim Med 2010 ;40(1):57-64

Katedra i Zakład Technologii Postaci Leku, Akademia Medyczna im. Piastów Slaskich we Wrocławiu.

The inhibitors of 3-hydroxy-3-methyloglutaryl coenzyme-A reductase are very popular in hipercholesterolemia therapy. According to current publications statins can be also used in other medical indications. In addition to fundamental cholesterol lowering activity statins give other effects as well, for example immunomodulating and anti-inflammatory effect, they promote bone mineralization. The anti-inflammatory activity has been described in few articles and clinical letters. Systemic or topical use of statins can be beneficial in alopecia, erythema, psoriasis. In the following paper prepared and tested formulations including simvastatin gel obtained from the Carbopolu 2020 with the addition of selected excipients: Tween 20, propylene glycol, glycerol, PEG 400. Particle size distributions, rheological characteristic of formulations and pharmaceutical availability were carried over to all formulations.
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May 2010
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