Publications by authors named "Arely Vergara-Castaneda"

14 Publications

  • Page 1 of 1

Inflammation Parameters Associated with Metabolic Disorders: Relationship Between Diet and Microbiota.

Metab Syndr Relat Disord 2021 Aug 16. Epub 2021 Aug 16.

Molecular Biology in Metabolic and Neurodegenerative Diseases Laboratory, Research Unit, High Specialty Regional Hospital of Ixtapaluca (HRAEI), Ixtapaluca, México.

The metabolic syndrome (MetS) includes numerous interrelated clinical, anthropometric, biochemical, and metabolic components and has become a public health problem due to its impact on morbimortality. Inflammation is a central mechanism underlying the etiology and clinical manifestations of MetS, contributing to its related pathological outcomes. Dietary patterns have been associated with the promotion of the diversity of microbiota in the digestive tract. Recently, research has focused on the importance of microbiota changes associated with MetS and inflammation. Other studies have been performed to understand the impact of prebiotics, probiotics, and synbiotics as allies on diet, inflammation, and MetS parameters. This review analyses the correlation between metabolic disorders, inflammation parameters, gut microbiota, and how diet has been involved as treatment of MetS and the modulation of inflammation and microbiota.
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http://dx.doi.org/10.1089/met.2021.0022DOI Listing
August 2021

Kinetics of HE4 and CA125 as prognosis biomarkers during neoadjuvant chemotherapy in advanced epithelial ovarian cancer.

J Ovarian Res 2021 Jul 19;14(1):96. Epub 2021 Jul 19.

Instituto Nacional de Cancerología, Colonia Seccion XVI, San Fernando 22, 14080, Tlalpan, Mexico City, Mexico.

Background: Ovarian cancer (OC) is considered the most lethal gynecological cancer, of which more than 65% cases are diagnosed in advanced stages, requiring platinum-based neoadjuvant chemotherapy (NACT).

Methods: A prospective-longitudinal study was conducted among women with advanced epithelial ovarian cancer (AEOC), III and IV stages, and treated with NACT, at the National Cancer Institute - Mexico, from July 2017 to July 2018. Serum samples were obtained for quantification of CA125 and HE4 using ELISA at the first and in each of the three NACT cycles. The therapeutic response was evaluated through standard tomography. We determined whether CA125 and HE4, alone or in combination, were associated with TR to NACT during follow up.

Results: 53 patients aged 38 to 79 years were included, 92.4% presented papillary serous subtype OC. Higher serum HE4 levels were observed in patients with non-tomographic response (6.89 vs 5.19 pmol/mL; p = 0.031), specially during the second (p = 0.039) and third cycle of NACT (p = 0.031). Multivariate-adjusted models showed an association between HE4 levels and TR, from the second treatment cycle (p = 0.042) to the third cycle (p = 0.033). Changes from baseline HE4 levels during the first cycle was negative associated with TR. No associations were found between CA125 and TR.

Conclusions: Serum HE4 levels were independently associated with TR among patients with AOEC treated with NACT, also a reduction between baseline HE4 and first chemotherapy levels was also independently associated with the TR. These findings might be relevant for predicting a lack of response to treatment.
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http://dx.doi.org/10.1186/s13048-021-00845-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8287739PMC
July 2021

Herpesvirus encephalitis diagnosed by polymerase chain reaction at the National Institute of Neurology of Mexico.

J Neurovirol 2021 Jun 8;27(3):397-402. Epub 2021 Apr 8.

Neuroimmunology Unit, National Institute of Neurology and Neurosurgery of Mexico (NINN), Insurgentes sur 3877, 14269, Mexico City, Mexico.

The frequency of central nervous system infections due to herpesvirus have been studied in various populations; however, studies in Mexican mestizo patients are scant. This paper documents the frequency of herpesvirus encephalitis in Mexican mestizo patients from the National Institute of Neurology and Neurosurgery (NINN) of Mexico. To study the frequency of herpetic viral encephalitis at the NINN in the period from 2004 to 2009. We reviewed clinical records from patients with clinically suspected encephalitis; polymerase chain reaction assays were done for detection of herpesviruses in cerebrospinal fluid (CSF) samples. The total number of patients studied was 502; in 59 (12%), the diagnosis of herpetic encephalitis was confirmed by PCR-based testing of CSF. Of them, 21 (36%) were positive for herpes simplex virus type 1, 15 (25%) for Epstein-Barr virus, 10 (17%) for varicella zoster virus, 8 (14%) for cytomegalovirus, 3 (5%) for human herpesvirus 6, and 2 (3%) for herpes simplex virus 2. Our results show a varied frequency of viral encephalitis in mestizo patients due to herpesviruses in a tertiary neurological center and point out the importance of modern molecular technology to reach the etiological diagnosis in cases of encephalitis.
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http://dx.doi.org/10.1007/s13365-020-00839-yDOI Listing
June 2021

In Silico Study of Polyunsaturated Fatty Acids as Potential SARS-CoV-2 Spike Protein Closed Conformation Stabilizers: Epidemiological and Computational Approaches.

Molecules 2021 Jan 29;26(3). Epub 2021 Jan 29.

Design, Isolation, and Synthesis of Bioactive Molecules Research Group, Chemical Sciences School, Universidad La Salle-México, Benjamín Franklin 45, Mexico City 06140, Mexico.

SARS-CoV-2 infects host cells by interacting its spike protein with surface angiotensin-converting enzyme 2 (ACE2) receptors, expressed in lung and other cell types. Although several risk factors could explain why some countries have lower incidence and fatality rates than others, environmental factors such as diet should be considered. It has been described that countries with high polyunsaturated fatty acid (PUFA) intake have a lower number of COVID-19 victims and a higher rate of recovery from the disease. Moreover, it was found that linoleic acid, an omega-6 PUFA, could stabilize the spike protein in a closed conformation, blocking its interaction with ACE2. These facts prompted us to perform in silico simulations to determine if other PUFA could also stabilize the closed conformation of spike protein and potentially lead to a reduction in SARS-CoV-2 infection. We found that: (a) countries whose source of omega-3 is from marine origin have lower fatality rates; and (b) like linoleic acid, omega-3 PUFA could also bind to the closed conformation of spike protein and therefore, could help reduce COVID-19 complications by reducing viral entrance to cells, in addition to their known anti-inflammatory effects.
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http://dx.doi.org/10.3390/molecules26030711DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7866518PMC
January 2021

Papel de las alteraciones del sueño durante la gestación en la programación del feto para el desarrollo de obesidad y enfermedades crónicas degenerativas.

Gac Med Mex 2019 ;155(4):423-427

Universidad La Salle, Facultad de Ciencias Químicas, Grupo de Investigación en Ciencias Básicas y Clínicas de la Salud, Ciudad de México. México.

Sleep disturbances are common in the third trimester of pregnancy and generate changes in the secretion of melatonin in pregnant women who sleep less than eight hours or have sleep disturbances, which promote various physiological changes in the mother that in turn result in low birth weight (LBW) in the fetus. LBW is associated with a phenomenon known as "metabolic programming," in which the fetus is subjected to a stressful situation that results in irreversible metabolic alterations that predispose it to the development of obesity in adulthood.
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http://dx.doi.org/10.24875/GMM.18004759DOI Listing
February 2020

Thyroid function in pediatric population with different nutritional status.

Bol Med Hosp Infant Mex 2018 ;75(5):279-286

Laboratorio de Obesidad y Enfermedades Metabólicas, Grupo de Investigación en Alimentos y Salud de la Facultad de Ciencias Químicas, Universidad La Salle, Ciudad de México, México.

Background: Thyroid dysfunction has been associated with the development of obesity. There are few studies describing their status in Mexican schoolchildren, in whom obesity and subclinical hypothyroidism (SCH) prevail.

Methods: Levels of stimulating thyroid hormone (TSH) and thyroid hormones (TH) were correlated with anthropometric variables as indicators of nutritional status in schoolchildren residents of Mexico City. The thyroid status and prevalences of SCH were compared between all the nutritional conditions, considering the degree of pubertal development.

Results: The mean of TSH was 2.96 ± 1.48 mIU/L, and the prevalence of SCH was 11.30%. TSH levels are higher in prepubertal (5.21 ±1.24 mIU/L (95% confidence interval [CI]: 3.72-6.69) versus pubertal children (2.96 ± 1.48 mIU/L [95% CI: 2.61-3.), as well as in children with obesity (3.5 ± 1.13 mIU/L [95% CI: 2.98-4.02]) versus normal weight children (2.43 ± 1.37 mIU/L [95% CI: 1.88 - 2.97]). The TH is similar in the whole population, although triiodothyronine total levels tend to be lower in malnourished children. There is a positive correlation between TSH levels and all anthropometric variables. The prevalences of SCH were higher in groups of children with overweight and obesity.

Conclusions: The body fat content is associated with thyroid status in Mexican schoolchildren. In addition, it is relevant to consider the degree of pubertal development for diagnosing hyperthyrotropinemia in children and adolescents.
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http://dx.doi.org/10.24875/BMHIM.M18000036DOI Listing
October 2019

Seroprevalence of anti-hepatitis E virus antibodies in domestic pigs in Mexico.

BMC Vet Res 2017 Sep 21;13(1):289. Epub 2017 Sep 21.

Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad de México, 04510, México.

Background: Hepatitis E virus (HEV) infection is one of the most common causes of acute liver diseases in humans worldwide. In developing countries, HEV is commonly associated with waterborne outbreaks. Conversely, in industrialized countries, HEV infection is often associated with travel to endemic regions or ingestion of contaminated animal products. Limited information on both, human and animal HEV infection in Mexico is available. As a consequence, the distribution of the virus in the country is largely unknown. Here, we assessed the seroprevalence of HEV among swine in different geographical regions in Mexico.

Methods: Seroprevalence of anti-HEV antibodies in swine herds in Mexico was evaluated in a representative sample including 945 pig serum specimens from different regions of the country using a commercial enzyme-linked immunosorbent assay (ELISA).

Results: The overall prevalence of anti-HEV antibodies in swine was 59.4%. The northern region of Mexico exhibited the highest seroprevalence in the country (86.6%), while the central and southern regions in Mexico showed lower seroprevalence, 42.7% and 51.5%, respectively.

Conclusions: In Mexico, HEV seroprevalence in swine is high. Importantly, northern Mexico showed the highest seroprevalence in the country. Thus, further studies are required to identify the risk factors contributing to HEV transmission among pigs in the country. Assessment of HEV human infection in the context of viral transmission in swine is required to better understand the epidemiology of hepatitis E in Mexico.
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http://dx.doi.org/10.1186/s12917-017-1208-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5609016PMC
September 2017

Cytokine expression at the anchor site in experimental Taenia solium infection in hamsters.

Vet Parasitol 2014 Mar 31;200(3-4):299-302. Epub 2013 Dec 31.

Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, México, DF, Mexico. Electronic address:

The establishment of Taenia solium adult parasite in the human intestine causes taeniosis. Importantly, the immunological mechanisms occurring at the interface between the parasite and its host are not fully known. The development of experimental animal models has facilitated the understanding of the host-parasite relationship. In this study we standardized a quantitative RT-PCR method for analyzing hamster messenger RNA for interferon-gamma (IFN-γ) and interleukins (IL): IL-4 IL-10, IL-12 and IL-13. This method was then used to evaluate the local cytokine response elicited against the adult parasite at the attachment site in the intestine of infected hamsters. The results showed an intense IFN-γ response, as well as an up-regulation of IL-4 as early as three days post-infection, permanence of IL-10 until the end of the experiment and down regulation of IL-12. These data are in agreement with a bias toward a Th-2 response as the infection progresses.
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http://dx.doi.org/10.1016/j.vetpar.2013.12.019DOI Listing
March 2014

Participation of CD161(+) and invariant natural killer T cells in pediatric asthma exacerbations.

Allergy Asthma Proc 2013 Jan-Feb;34(1):84-92

Departamento de Investigaciones Inmunológicas, Instituto de Diagnóstico y Referencia Epidemiológicos, Mexico City, Mexico.

Asthma has been defined as a disease of chronic airway inflammation in which many cells and cellular products participate with variable degrees of airflow obstruction and hyperresponsiveness that lead to recurrent episodes of wheezing, breathlessness, chest tightness, and coughing. Prominent among these cellular elements are two cell types referred to as the invariant natural killer T (iNKT) cells and a subpopulation of T cells expressing the molecule CD161, which are both thought to play a role in the pathogenesis of asthma. Although the presence of iNKT and other CD161(+) cells in murine models has been associated with asthma, relatively few studies have been performed in the adult patient with asthma that have been often conflicting and even fewer studies are available in children. The present study was performed to investigate the peripheral blood frequencies of iNKT and CD161(+) T cells in children with asthma. A total of 35 children, 19 stable asthmatic patients, 6 who had experienced an asthmatic attack within 24 hours and had not received any treatment, and 10 healthy controls, aged 6-12 years, were enrolled in the study. iNKT and CD161(+) T-cell frequencies in blood were measured together with quantitative levels of IL-4 and interferon (IFN) γ using a cytofluorimetric approach. The results show that iNKT cells are increased in pediatric asthmatic patients undergoing exacerbations of asthma. These cells also produced less IFN-γ and more IL-4 than children with stable asthma and in healthy control children. These results suggest that iNKT cells might participate in the development of the asthmatic exacerbations. The increased production of IL-4 in conjunction with the decrease of IFN-γ may be mechanistically responsible, at least partially, for the heightening of the immunologic response leading to the asthmatic attack in children. Knowledge of these interactive mechanisms involving the iNKT cell and our understanding of its role in the exacerbation of asthma hold great promise in the development of better diagnostic predictive markers of disease progression as well as new forms of therapeutic interventions.
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http://dx.doi.org/10.2500/aap.2013.34.3619DOI Listing
August 2013

Genetic diversity among multidrug-resistant Mycobacterium tuberculosis strains in Mexico.

Infect Genet Evol 2013 Mar 16;14:434-43. Epub 2013 Jan 16.

Instituto de Diagnóstico y Referencia Epidemiológicos, Secretaría de Salud, Mexico City, Mexico.

Tuberculosis is an important public health problem in Mexico. However, limited information about the genetic diversity of Mycobacterium tuberculosis strains circulating in the country is available. In this work, 109 multidrug-resistant (MDR) M. tuberculosis isolates collected in 23 different states of Mexico in 2003 were retrospectively characterized by spoligotyping and MIRU-VNTRs. All isolates, except for a single cluster containing two strains (subcluster E1), were split when information from the 12-loci MIRUs and spoligo-pattern was simultaneously analyzed. The discriminative power of 12-loci MIRU-VNTR and spoligotyping, by the Hunter-Gaston index, were 0.9998 and 0.9011, respectively. These findings suggest that almost all cases were epidemiologically unrelated. Instead, the genetic variations observed among these strains are suggestive of emergence of acquired drug-resistance during the course of treatment. The results suggest a high degree of genetic variability and a high frequency of SIT53 (T1 family) spoligotype among the MDR M. tuberculosis isolates included in the study.
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http://dx.doi.org/10.1016/j.meegid.2012.12.024DOI Listing
March 2013

Rapid hepatitis C virus divergence among chronically infected individuals.

J Clin Microbiol 2013 Feb 5;51(2):629-32. Epub 2012 Dec 5.

Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico.

Here, we analyze the viral divergence among hepatitis C virus (HCV) chronic cases infected with genotype 1. The intrahost viral evolution was assessed by deep sequencing using the 454 Genome Sequencer platform. The results showed a rapid nucleotide sequence divergence. This notorious short-term viral evolution is of the utmost importance for the study of HCV transmission, because direct links between related samples were virtually lost. Thus, rapid divergence of HCV significantly affects genetic relatedness studies and outbreak investigations.
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http://dx.doi.org/10.1128/JCM.03042-12DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3553878PMC
February 2013

Epidemiology of varicella in Mexico.

J Clin Virol 2012 Sep 30;55(1):51-7. Epub 2012 Jun 30.

Universidad Nacional Autónoma de México, México City, Mexico.

Background: The epidemiological patterns of varicella-zoster virus (VZV) infection, which are strongly associated with climate, are characterized by more frequent infections occurring among children in temperate regions than in the tropics. In temperate regions, varicella exhibits a seasonal cyclic behavior in which the number of cases increases significantly during the winter and spring seasons, further supporting the role of environmental factors in disease transmission. However, the underlying mechanisms responsible for this distinctive behavior are not fully understood. In Mexico, information regarding the epidemiology of varicella is scarce, and the distribution of VZV infection has not been analyzed.

Objectives: In this article we investigate the epidemiological patterns of varicella in Mexico and their relationship with different environmental and demographic factors.

Study Design: A retrospective study was conducted using the data reported by the National Center of Epidemiological Surveillance and Disease Control. The overall varicella incidence was calculated and associated with temperature, overcrowding, age, gender and population density.

Results: The epidemiology of varicella showed an intriguing pattern, in which warmer regions were characterized by higher incidences than in temperate regions. Young children were the most affected age group. There was no correlation between varicella incidence and overcrowding or population density.

Conclusions: The epidemiology of varicella in Mexico significantly departs from the characteristic patterns observed in other tropical latitudes, with some features resembling those commonly associated with temperate regions.
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http://dx.doi.org/10.1016/j.jcv.2012.06.004DOI Listing
September 2012

Overweight, obesity, high blood pressure and lifestyle factors among Mexican children and their parents.

Environ Health Prev Med 2010 Nov 15;15(6):358-66. Epub 2010 May 15.

Asociación Mexicana para la Prevención de Insuficiencia Cardiaca AC, Mexico City, Mexico.

Objective: The objective of this study was to identify associations in the prevalence of overweight, obesity and high blood pressure between children and their parents, as well as their eating and physical patterns.

Methods: In this cross-sectional study, we obtained data on 83 pairs of school-aged children and one of their parents relating to dietary habits and various physical parameters, including the body mass index (BMI) and blood pressure of the children, which were adjusted by age and gender. Both the children and the parents were asked to complete a questionnaire aimed at providing measures of eating behavior. The questions focused on the consumption of fruit and vegetables and soda drinks as well as on physical activity patterns. Parent BMI was calculated from self-reported height and weight values.

Results: Obesity was diagnosed in 10.8% of the children, and the prevalence of overweight was 28.9%. There was a relationship between a child's weight status and that of his/her parent according to the BMI; 45% of overweight/obese children had overweight/obese parents. In addition, a parent's fruit and vegetable consumption was associated with his/her child's fruit and vegetable consumption (r = 0.47, p < 0.001), and both were associated with soda drink consumption in both parents and children (r = 0.30, p < 0.001).

Conclusion: Our results confirmed that there is a relationship between the weight status, fruit and vegetable consumption and soda drink intake of children and those of their parents.
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http://dx.doi.org/10.1007/s12199-010-0151-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2955902PMC
November 2010

Waist circumference and fat intake are associated with high blood pressure in Mexican children aged 8 to 10 years.

J Am Diet Assoc 2009 Jun;109(6):996-1003

Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, México City, Mexico.

Background: Elevated blood pressure at a young age is a predictor of blood pressure elevation later in life and has been associated with being overweight. However, it seems that there are other factors related to hypertension in childhood.

Objective: To determine the anthropometric, physical activity, and dietary factors associated with both systolic and diastolic hypertension in a group of Mexican school-aged children.

Design: In this cross-sectional descriptive study, blood pressure, anthropometrics, physical activity, and dietary intake (assessed with a multiple-pass 24-hour dietary recall) evaluations were performed on school-aged children from September 2005 to July 2006.

Subjects: Children aged 8 to 10 years (626 boys and 613 girls) from a convenience sample of 23 public schools of low socioeconomic status in Mexico City were included. Systolic and diastolic hypertension were defined as blood pressure > or = 95th percentile for age, sex, and height, without diastolic or systolic prehypertension or hypertension, respectively. Mixed hypertension was defined as the presence of both systolic and diastolic hypertension.

Statistical Analysis: Descriptive statistics, chi(2) tests, analysis of variance, post hoc analysis with Bonferroni method, and logistic regression analysis were performed. Continuous variables are presented as mean+/-standard deviation, categorical variables as percentages, and the association measures as adjusted odds ratios (ORs). A P value < 0.05 was considered statistically significant, and 95% confidence intervals were calculated for adjusted ORs.

Results: The dietary intake analysis showed that both boys and girls had low fiber intake (< 25 g/day) as well as an excessive mean consumption of total and saturated fat (> 35% and > 10% of daily energy, respectively). From the total group of children, 3.6% had systolic hypertension, 14.2% diastolic hypertension, and 22.8% mixed hypertension. The higher values of weight, body mass index, waist circumference, and prevalence of overweight and obesity were more commonly seen among children with mixed hypertension, followed by children in the systolic group. Subjects with diastolic and mixed hypertension had an elevated intake of dietary total fat (37.7% and 38.2% of daily energy intake, respectively). Moreover, an adjusted OR of 1.06 (95% confidence interval 1.01 to 1.11) for systolic hypertension per increase of 1 cm in waist diameter was found and total fat intake > 35% of the daily energy showed an adjusted OR of 2.61 (95% confidence interval 1.27 to 5.36) for diastolic hypertension.

Conclusions: In a sample of Mexican children of low socioeconomic status, the prevalence of hypertension was high. Waist circumference was the main factor associated with systolic hypertension, and higher intake of total fat was related to diastolic hypertension.
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http://dx.doi.org/10.1016/j.jada.2009.03.011DOI Listing
June 2009
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