Publications by authors named "Ao Zeng"

13 Publications

  • Page 1 of 1

Formation of filamentous fungal pellets in aerobic granular sludge via reducing temperature and dissolved oxygen: Characteristics of filamentous fungi and denitrification performance.

Bioresour Technol 2021 Jul 26;332:125056. Epub 2021 Mar 26.

School of Civil Engineering, Southeast University, Nanjing 210096, China. Electronic address:

A lab-scale sequencing batch reactor (SBR) using glucose as carbon source was operated for 500 days to investigate the formation of filamentous organisms and their function on stability of AGS system. After 250 days' stable operation under conditions of 25 ± 2 °C and dissolved oxygen (DO) of 4-5 mg/L (stage I), the temperature and DO were reduced to 10 ± 2 °C and DO of 1-2 mg/L until 280 days (stage II), to induce the growth of filamentous microorganisms. After that until 500 days (stage III), overgrowth of filamentous microorganisms with relative abundances of up to 19.46%, formation of black filamentous fungal pellets, and reconstruction of AGS granules were observed in turn. The relation between settling of AGS (SVI 30-72 mL/g) and filamentous microorganisms was revealed. Filamentous pellets were purified and identified as fungal Bradymyces and Knufia, with stronger denitrification performance on nitrite than nitrate. The results indicated that filamentous fungal pellets contributed to good sludge settling performance and promoted the denitrification process in AGS.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biortech.2021.125056DOI Listing
July 2021

Seroprevalence and risk factors of Toxoplasma gondii infection in goats in China from 2010 to 2020: A systematic review and meta-analysis.

Prev Vet Med 2021 Jan 5;186:105230. Epub 2020 Dec 5.

College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong Province, 266109, PR China. Electronic address:

Toxoplasma gondii is a widely distributed protozoan parasite that can infect a variety of warm-blooded animals including humans and goats. This is the first meta-analysis to assess the overall seroprevalence and potential risk factors of T. gondii infection among goats in China. Databases including the VIP Chinese Journal Databases (VIP), the China National Knowledge Infrastructure (CNKI) database, WanFang, ScienceDirect, and PubMed were searched comprehensively for relevant studies published from 2010 to date. A random-effects model was used to calculate pooled seroprevalence estimates with 95 % confidence intervals (CI) and data were analyzed from 13 provinces in China. The pooled T. gondii seroprevalence in goats was estimated as 9.9 %. Goats T. gondii had the highest seroprevalence in Southwestern China (13.3 %) and the lowest in Northeastern China (7.3 %). The seroprevalence in the sub-group before 2012 (8.0 %) was lower than that in 2012-2015 (15.2 %) and 2016 or later (9.2 %). Goats aged > 12-months had a higher seroprevalence (11.7 %) than ≤ 12-months (10.7 %). The seroprevalence in female goats was 12.5 % and 12.2 % in male goats. In season subgroups, the seroprevalence was lowest in autumn (6.1 %). Naturally grazed goats had a seroprevalence of 8.1 % of infection and 7.8 % in intensively fed goats. We also evaluated the effects of geographical and climate variables on the pooled seroprevalence of T. gondii in goats in China. The results indicated that toxoplasmosis is widely distributed in goats in China. In the context of ensuring animal welfare, we suggest the continued promotion of the transition from natural grazing to intensive agriculture to strengthen disease prevention. Prevention would be better carried out in areas suitable for the survival and reproduction of T. gondii. Breeders need to regularly disinfect the feeding areas and pens or other areas of goat activity thoroughly to reduce the impact of environmental factors responses on the infection of goats to T. gondii.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.prevetmed.2020.105230DOI Listing
January 2021

Prevalence and Risk Factors of Infection Among Five Wild Rodent Species from Five Provinces of China.

Vector Borne Zoonotic Dis 2021 Feb 7;21(2):105-109. Epub 2020 Dec 7.

College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, China.

, an intracellular zoonotic parasite, can infect humans and various animals worldwide. Wild rodents plan an important role as intermediate hosts of . Some studies on from wild rodents have been published, but the investigation data of in wild rodents in China are limited. Therefore, brain tissue samples from 382 wild rodents in four provinces and one autonomous region of China were screened by PCR amplification of B1 gene. Furthermore, the wild rodents were identified as five species based on their morphological characteristics, including ( = 35 from Heilongjiang), ( = 81 from Inner Mongolia), ( = 12 from Heilongjiang), ( = 2 from Heilongjiang;  = 99 from Zhejiang;  = 54 from Shanxi), and ( = 99 from Guangxi). The overall prevalence of in these wild rodents was 5.24% (20/382) in this study. At different regions, the highest prevalence of was in Guangxi (12.12%) compared with other regions (0% in Heilongjiang; 2.47% in Inner Mongolia; 2.02% in Zhejiang; 7.41% in Shanxi). At different climates, the higher prevalence was found in temperate/mesothermal climates (7.07%) compared with continental/microthermal climates (3.26%). Also, the results showed that had the highest prevalence of infection (12.12%) among the rodent species sampled. Moreover, sampling year was significantly related to the prevalence of in investigated wild rodents ( = 0.0117). This is the first report of in wild rodents from Zhejiang, Guangxi, Shanxi, Heilongjiang provinces and Inner Mongolia autonomous region in China, providing the fundamental information for further prevention and control of toxoplasmosis in China.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1089/vbz.2020.2658DOI Listing
February 2021

Correlation of microbiota in the gut of fish species and water.

3 Biotech 2020 Nov 12;10(11):472. Epub 2020 Oct 12.

Department of Animal Nutrition, Hunan Institute of Microbiology, No 18 Xinkaipu Road, Changsha, 410009 Hunan China.

To analyze the intestinal microbiota diversity of several important economic fishes in the Loudi area and its correlation with the microbiota of water environment, the high-throughput sequencing based on the bacteria 16S rRNA was used to analyze the intestinal microbiota diversity in fish intestines and water. The results revealed that half of the OTUs in the water sample could be detected in the fish intestine, the proportion of shared OTUs in the intestines of and water samples was only 22%, and the unique OTU in the LC group was relatively the highest in the fish intestinal group. It can be seen from the analysis in NMDS analysis, the distance between group and water group is relatively farthest. has the highest microbiota richness and diversity ( < 0.05), while the water samples have the lowest microbiota richness ( < 0.05). Firmicutes, and are the prevalent taxonomic unit in the and groups, is the prevalent genera in the group, Proteobacteria and Cyanobacteria have a high relative abundance ratio in the group, and the prevalent taxonomic unit in the water sample group are , Bacteroidetes and Actinobacteria. In conclusion, fish species have different prevalent microbiota. There are a strong correlation between fish intestinal microbiota and the water environment, and the fish with a weak correlation is . Results of this study will contribute to the prevention and treatment of fish diseases and the fish ecological culturing in Loudi area.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s13205-020-02461-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7550513PMC
November 2020

Carbon Quantum Dots: In vitro and in vivo Studies on Biocompatibility and Biointeractions for Optical Imaging.

Int J Nanomedicine 2020 28;15:6519-6529. Epub 2020 Aug 28.

School of Basic Medical Sciences, Affiliated Stomatology Hospital, Guangzhou Medical University, Guangzhou 510182, People's Republic of China.

Background: Understanding the biocompatibility and biointeractions of nano-carbon quantum dots (nano-CQDs) in vitro and in vivo is important for assessing their potential risk to human health. In the previous research, the physical properties of CQDs synthesized by the laser ablation in liquid (LAL) method were analyzed in detail; however, possible bioapplications were not considered.

Materials And Methods: CQDs were prepared by LAL and characterized by atomic force microscopy, fluorescence lifetime, absorption spectrum, Fourier-transform infrared spectroscopy, and dynamic light scattering. Their biocompatibility was evaluated in vitro using assays for cytotoxicity, apoptosis, and biodistribution and in vivo using immunotoxicity and the relative expression of genes. Cells were measured in vitro using fluorescence-lifetime imaging microscopy to analyze the biointeractions between CQDs and intracellular proteins.

Results: There were no significant differences in biocompatibility between the CQDs and the negative control. The intracellular interactions had no impact on the optical imaging of CQDs upon intake by cells. Optical imaging of zebrafish showed the green fluorescence was well dispersed.

Conclusion: We have demonstrated that the CQDs have an excellent biocompatibility and can be used as efficient optical nanoprobes for cell tracking and biomedical labeling except for L929 and PC-3M cells.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/IJN.S257645DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7468940PMC
December 2020

Prevalence of Cryptosporidium in pigs in China: A systematic review and meta-analysis.

Transbound Emerg Dis 2020 Aug 20. Epub 2020 Aug 20.

College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, PR China.

Cryptosporidium is an important zoonotic parasite that can infect a variety of hosts, including pigs and humans, through water and food. Many studies on Cryptosporidium infection in pigs have been reported worldwide. However, the meta-analysis of Cryptosporidium infection in pigs in China has not been published. This study retrieved articles related to Cryptosporidium in pigs in China by using four databases: Chinese National Knowledge Infrastructure (CNKI), PubMed, VIP Chinese journal database and Wanfang Data. We retrieved 40 studies related to Cryptosporidium infection in China, and those articles were harvested from the inception to 1 January 2020. We estimated that the overall prevalence of pigs with Cryptosporidium in the selected period was 12.2% (4,349/30,404). In the sampling year subgroup, the prevalence rate after 2010 was the lowest at 8.7% (2,087/18,100). In Northern China, the Cryptosporidium prevalence was 47.9% (34/71). By contrast, the prevalence of Cryptosporidium in Southwestern China was only 6.9% (778/6,445). The infection rate of Cryptosporidium in diarrhoea pigs of 15.6% (74/384) was higher than that in non-diarrhoea pigs at 10.8% (378/2,840). Among the four age groups, the prevalence of weaning pigs of 16.2% (530/3,243) was the highest, and the difference was significant (p < .05). The prevalence of Cryptosporidium in extensive farming was 25.7% (660/3,121), which was significantly higher than in intensive farming 8.7% (566/6,336), and the prevalence of infection was related to the farming modes (p < .05). We also analysed the impact of different geographic factor subgroups (longitude, latitude, precipitation, temperature, humidity, climate and altitude) on the prevalence of pigs. The results showed that cryptosporidiosis was widespread in pigs in China. We suggest that appropriate control schemes should be developed according to the differences in breeding patterns and geographic conditions in different regions, and effective management measures should be developed to reduce the spread between pigs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/tbed.13806DOI Listing
August 2020

The global seroprevalence of Toxoplasma gondii in deer from 1978 to 2019: A systematic review and meta-analysis.

Acta Trop 2020 May 17;208:105529. Epub 2020 May 17.

College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong Province, 266109, China. Electronic address:

Toxoplasma gondii infecting virtually all warm-blooded animals, including humans, is an intracellular protozoan parasite. The immunocompromised patients or pregnant women infected by Toxoplasma usually can cause encephalitis or abortion. Deer are also the important intermediate host of the parasite and people can be infected by ingesting the undercooked venison. Therefore, to raise the attention of the researchers and T. gondii infection on people in deer, we conducted the meta-analysis. All potential publications on the T. gondii infection in deer were retrieved by searching the PubMed, ScienceDirect, Springer- Link, China National Knowledge Infrastructure (CNKI), WanFang, and VIP Chinese Journal Databases. Finally, a total of 48 publications were included. The pooled seroprevalence of T. gondii infection in deer was 22.92% (95% CI 17.89-28.38) calculated by the random effect model. The seroprevalence of T. gondii infection in deer was the lowest in Asia (12.72%, 95% CI 6.29-20.89), and the highest in North America (32.21%, 95% CI 20.32-45.39). The highest point estimate of deer T. gondii in detection method subgroup was using MAT (31.28%, 95% CI 20.15-43.61). The estimated pooled seroprevalence of T. gondii infection in elk (63.50%, 95% CI 50.01-76.01) was higher than other types of deer. Our meta-regression analysis found that the subgroups including region, sampling year, age, climate, and deer species may be the main heterogeneous source in our meta-analysis. It is necessary to monitor the seroprevalence of T. gondii infection in deer at all times. Furthermore, people should be taken to precautions when ingesting the venison by cooking it well before serving to prevent the T. gondii infection via eating venison.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.actatropica.2020.105529DOI Listing
May 2020

Post-Translational Modifications Aid Archaeal Survival.

Biomolecules 2020 04 10;10(4). Epub 2020 Apr 10.

State Key Laboratory of Agro-Biotechnology and Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Biological Sciences, China Agricultural University, No.2 Yuan-Ming-Yuan West Road, Beijing 100193, China.

Since the pioneering work of Carl Woese, Archaea have fascinated biologists of almost all areas given their unique evolutionary status, wide distribution, high diversity, and ability to grow in special environments. Archaea often thrive in extreme conditions such as high temperature, high/low pH, high salinity, and anoxic ecosystems. All of these are threats to the stability and proper functioning of biological molecules, especially proteins and nucleic acids. Post-translational modifications (PTMs), such as phosphorylation, methylation, acetylation, and glycosylation, are reportedly widespread in Archaea and represent a critical adaptive mechanism to extreme habitats. Here, we summarize our current understanding of the contributions of PTMs to aid in extremophile survival, with a particular focus on the maintenance of genome stability.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/biom10040584DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7226565PMC
April 2020

Ligustilide Inhibited Rat Vascular Smooth Muscle Cells Migration via c-Myc/MMP2 and ROCK/JNK Signaling Pathway.

J Food Sci 2019 Dec 24;84(12):3573-3583. Epub 2019 Nov 24.

School of Life Sciences and Biopharmaceutics, Guangdong Pharmaceutical Univ., Guangzhou, 510006, China.

Vascular smooth muscle cells (VSMCs) excessive migration, a basic change of pathological intimal thickening, can lead to serious cardiovascular diseases such as atherosclerosis, myocardial infarction, and stroke. Ligustilide (LIG), the main active ingredient of angelica volatile oil, has been demonstrated to exert protective effects on the cardiovascular and cerebrovascular, circulatory system, and immune function. However, whether it protects against intimal thickening and VSMCs excessive migration and its underlying mechanism remains largely unknown. The aim of this study is to investigate the effect of LIG on VSMCs migration and its underlying mechanism. The protective effect of LIG on VSMCs excessive migration was assessed using an atherosclerotic spontaneously hypertensive rat model and an angiotensin II (AngII)-induced VSMCs migration model. The results showed that LIG exerted a protective effect against pathological intimal thickening as demonstrated by decreasing VSMCs migration in vivo and in vitro. In vivo, intimal thickening and VSMCs migration were inhibited and LIG performed a suppressive effect on the expression of c-Myc protein while enhanced phenotypic transformation related proteins α-SMA expression. Meanwhile, the administration of LIG significantly lowered the blood pressure and blood lipids level in atherosclerotic spontaneously hypertensive rats. In vitro, LIG suppressed AngII-induced VSMCs migration and downregulated the expression of migration related protein c-Myc, MMP2, ROCK1, ROCK2, p-JNK, and JNK. These findings suggested the protective effect of LIG on VSMCs migration was associated with the decrement of c-Myc/MMP2 signaling pathway and ROCK-JNK signaling pathway. Thus, LIG may serve as a novel therapeutic agent for preventing cardiovascular disease.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/1750-3841.14936DOI Listing
December 2019

Effects of Debaryomyces hansenii treatment on intestinal mucosa microecology in mice with antibiotic-associated diarrhea.

PLoS One 2019 14;14(11):e0224730. Epub 2019 Nov 14.

Hunan University of Chinese Medicine, Changsha, Hunan Province, China.

Aim: To confirm the effects of Debaryomyces hansenii on intestinal microecology in mice with antibiotic-associated diarrhea (AAD).

Methods: This study took the mucosal microecology as the entry point and an antibiotic mixture was used to induce diarrhea in mice. D. hansenii suspension was used to treat the mice and the bacterial communities of mucosa was analyzed using high-throughput sequencing.

Results: The Shannon-Wiener index indicated that the sequencing depth is reasonable and reflected the majority of microbial information. The principal coordinate analysis results showed that mice in the treatment group and the normal group had a similar microbial community structure, while differences in microbial community structure were observed between the model group and the treatment group. The inter-group bacterial structures were analyzed at the phylum level and genus level. The results revealed that antibiotic treatment increased the proportion of Proteobacteria and decreased the proportion of Bacteroides, while D. hansenii treatment inhibited the increase in Proteobacteria. Linear discriminant analysis coupled with effect size measurements (LEfSe) suggested d that the beneficial bacteria Candidatus Arthromitus were the only common bacteria in the normal group (P<0.05).

Conclusion: The treatment with D.hansenii could contribute to the maintenance of the structure of the mucosal microbiota in comparison with the normal group and inhibit the proliferation of opportunistic bacteria. However, high-dose antibiotic treatment causes mucosal dysbiosis and the proliferation of opportunistic bacteria during the self-recovery period, such as Pseudoalteromonas, Alteromonas, Vibrio.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0224730PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6855419PMC
March 2020

Inorganic fullerene-like molybdenum selenide with good biocompatibility synthesized by laser ablation in liquids.

Nanotechnology 2018 Jul 1;29(29):295604. Epub 2018 May 1.

State Key Laboratory of Optoelectronic Materials and Technologies, Nanotechnology Research Center, School of Materials Science & Engineering, School of Physics, Sun Yat-sen University, Guangzhou 510275, Guangdong, People's Republic of China.

The fabrication of inorganic fullerene-like nanoparticles (IFNPs) is an attractive idea due to their unique structures and various potential applications. To date, IFNPs have been made from numerous compounds with layered two-dimensional structures, based on various synthetic methods. Here we have demonstrated for the first time that inorganic fullerene-like molybdenum selenide nanoparticles (MoSe IFNPs) can be synthesized by laser ablating a molybdenum selenide target in 30 vol % ethanol/water mixture at ambient temperature and pressure. The formation mechanism was proposed to elucidate the production of MoSe IFNPs in the process of laser ablation in liquids (LAL). The appropriate solvent facilitates the condensation of the plasma plume created by LAL to planar MoSe. Then, laser-induced high temperature and high pressure lead to the formation of a vacancy in the planar MoSe, causing the generation of nucleation and growth of the MoSe IFNPs. In addition, a CCK-8 (cell counting kit-8) assay and a cell viability assay were performed to examine the cytotoxic behavior and the effect on cell viability of MoSe IFNPs. The results show that MoSe IFNPs are reasonably nontoxic and biocompatible with the given cells, showing they have significant potential in biomedical applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1088/1361-6528/aac1b1DOI Listing
July 2018

Comparison and strategy of nitrogen removal at different low temperatures in a pilot-scale A/O system.

Environ Technol 2019 Aug 5;40(19):2473-2481. Epub 2018 Mar 5.

a School of Civil Engineering and Architecture, Wuhan University of Technology , Wuhan , People's Republic of China.

To ensure low effluent total nitrogen (TN ≤ 15 mg L) and NH-N( ≤ 5 mg L) at low temperatures (8.8-14.7°C), a pilot-scale A/O system with a low return activated sludge (200%) was employed to seek the optimal operation strategy. In the 90 days' experiment, the tests were separated into three stages (14.2°C, 11.0°C, and 9.1°C), and the 49 samples were analysed. Our results showed that the nitrification was remarkably inhibited when the temperature was below 10°C, while the denitrification was remarkably inhibited at the temperatures of 10-15°C. Once the effluent NH-N concentration was over 2.5 mg L, or concentration was over 13 mg L, the effluent TN would be in excess of 15 mg L. When the temperature was over 10°C, increasing dissolved oxygen (DO) was an optimal strategy to reduce the effluent NH-N; nevertheless, when the temperature was below 10°C, simultaneously increasing DO and MLVSS would be carried out due to the low max. nitrification rates (36.0 mg (g d)). If the carbon source (CODcr/ ratio ≥ 7.3) was enough, increasing MLVSS was an effective method to relieve the negative influence of low temperature on denitrification.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/09593330.2018.1444099DOI Listing
August 2019

Targeted metabolic profiling of hepatocellular carcinoma and hepatitis C using LC-MS/MS.

Electrophoresis 2013 Oct 1;34(19):2910-7. Epub 2013 Sep 1.

Department of Chemistry, Purdue University, West Lafayette, IN, USA.

Hepatitis C virus (HCV) infection of the liver is a global health problem and a major risk factor for the development of hepatocellular carcinoma (HCC). Sensitive methods are needed for the improved and earlier detection of HCC, which would provide better therapy options. Metabolic profiling of the high-risk population (HCV patients) and those with HCC provides insights into the process of liver carcinogenesis and possible biomarkers for earlier cancer detection. Seventy-three blood metabolites were quantitatively profiled in HCC (n = 30) and cirrhotic HCV (n = 22) patients using a targeted approach based on LC-MS/MS. Sixteen of 73 targeted metabolites differed significantly (p < 0.05) and their levels varied up to a factor of 3.3 between HCC and HCV. Four of these 16 metabolites (methionine, 5-hydroxymethyl-2'-deoxyuridine, N2,N2-dimethylguanosine, and uric acid) that showed the lowest p values were used to develop and internally validate a classification model using partial least squares discriminant analysis. The model exhibited high classification accuracy for distinguishing the two groups with sensitivity, specificity, and area under the receiver operating characteristic curve of 97%, 95%, and 0.98, respectively. A number of perturbed metabolic pathways, including amino acid, purine, and nucleotide metabolism, were identified based on the 16 biomarker candidates. These results provide a promising methodology to distinguish cirrhotic HCV patients, who are at high risk to develop HCC, from those who have already progressed to HCC. The results also provide insights into the altered metabolism between HCC and HCV.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/elps.201300029DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3826436PMC
October 2013