Publications by authors named "Ao Gong"

3 Publications

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Damage to the blood‑brain barrier and activation of neuroinflammation by focal cerebral ischemia under hyperglycemic condition.

Int J Mol Med 2021 07 3;48(1). Epub 2021 Jun 3.

Department of Pathology, School of Basic Medical Science, Ningxia Medical University, Ningxia Key Laboratory of Cerebrocranial Diseases, Incubation Base of National Key Laboratory, Yinchuan, Ningxia 750004, P.R. China.

Hyperglycemia aggravates brain damage caused by cerebral ischemia/reperfusion (I/R) and increases the permeability of the blood‑brain barrier (BBB). However, there are relatively few studies on morphological changes of the BBB. The present study aimed to investigate the effect of hyperglycemia on BBB morphological changes following cerebral I/R injury. Streptozotocin‑induced hyperglycemic and citrate‑buffered saline‑injected normoglycemic rats were subjected to 30 min middle cerebral artery occlusion. Neurological deficits were evaluated. Brain infarct volume was assessed by 2,3,5‑triphenyltetrazolium chloride staining and BBB integrity was evaluated by Evans blue and IgG extravasation following 24 h reperfusion. Changes in tight junctions (TJ) and basement membrane (BM) proteins (claudin, occludin and zonula occludens‑1) were examined using immunohistochemistry and western blotting. Astrocytes, microglial cells and neutrophils were labeled with specific antibodies for immunohistochemistry after 1, 3 and 7 days of reperfusion. Hyperglycemia increased extravasations of Evan's blue and IgG and aggravated damage to TJ and BM proteins following I/R injury. Furthermore, hyperglycemia suppressed astrocyte activation and damaged astrocytic endfeet surrounding cerebral blood vessels following I/R. Hyperglycemia inhibited microglia activation and proliferation and increased neutrophil infiltration in the brain. It was concluded that hyperglycemia‑induced BBB leakage following I/R might be caused by damage to TJ and BM proteins and astrocytic endfeet. Furthermore, suppression of microglial cells and increased neutrophil infiltration to the brain may contribute to the detrimental effects of pre‑ischemic hyperglycemia on the outcome of cerebral ischemic stroke.
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http://dx.doi.org/10.3892/ijmm.2021.4975DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175066PMC
July 2021

Biosynthesis of biocompatibility AgSe quantum dots in Saccharomyces cerevisiae and its application.

Biochem Biophys Res Commun 2021 Mar 28;544:60-64. Epub 2021 Jan 28.

Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Wuhan University, Wuhan, 430072, China. Electronic address:

As fluorescence in the second near-infrared window (NIR-II, 1000-1400 nm) could image deep tissue with high signal-to-noise ratios compared with that in NIR-I (750-900 nm), AgSe quantum dots (QDs) with fluorescence in the NIR-II could be ideal fluorophores. Here, we described a biosynthesis method to prepare the AgSe QDs by using temporally coupling the irrelated biochemical reactions, whose photoluminescence (PL) emission can reach NIR-II. The nanoparticles were characterized by transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HRTEM), energy-dispersive X-ray spectroscopy (EDX) and X-ray diffraction (XRD). The results showed that the nanoparticles obtained by extracellular purification were AgSe QDs with a uniform size of 3.9 ± 0.6 nm. In addition, the fluorescence intensity of Saccharomyces cerevisiae was improved successfully by nearly 4-fold by constructed engineering strain. In particular, the biosynthesis of AgSe QDs had good biocompatibility because it was capped by protein. Furthermore, investigating the toxicity of AgSe on cells and NIR images of nude mice showed that the AgSe synthesized using S. cerevisiae had low toxicity and could be used for in vivo imaging. In this work, the synthesis pathway of biocompatible AgSe was broadened and laid a foundation for the enlarged applicability of bioimaging in the biosynthesis of NIR-II QDs.
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http://dx.doi.org/10.1016/j.bbrc.2021.01.071DOI Listing
March 2021

Remote activation of nanoparticulate biomimetic activity by light triggered pH-jump.

Chem Commun (Camb) 2018 Aug 18;54(62):8641-8644. Epub 2018 Jul 18.

Research Institute for Biomimetics and Soft Matter, Fujian Provincial Key Laboratory for Soft Functional Materials Research, College of Physical Science and Technology, College of Materials, Xiamen University, Xiamen 361005, China.

Herein, we report a facile, efficient and versatile method for the photo-regulation of pH-dependent activities of artificial enzymes by incorporating flash photolysis reagents. Under light excitation, a persistent pH shift is achieved by proton release from photosensitive 2-nitrobenzaldehyde. Following such change, the controlled activation of oxidase-like activity of nanoceria is successfully demonstrated.
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http://dx.doi.org/10.1039/c8cc04279aDOI Listing
August 2018