Publications by authors named "Anthony Attwood"

4 Publications

  • Page 1 of 1

Genetic determinants of major blood lipids in Pakistanis compared with Europeans.

Circ Cardiovasc Genet 2010 Aug 22;3(4):348-57. Epub 2010 Jun 22.

Center for Non-Communicable Diseases Karachi, Pakistan.

Background: Evidence is sparse about the genetic determinants of major lipids in Pakistanis.

Methods And Results: Variants (n=45 000) across 2000 genes were assessed in 3200 Pakistanis and compared with 2450 Germans using the same gene array and similar lipid assays. We also did a meta-analysis of selected lipid-related variants in Europeans. Pakistani genetic architecture was distinct from that of several ethnic groups represented in international reference samples. Forty-one variants at 14 loci were significantly associated with levels of HDL-C, triglyceride, or LDL-C. The most significant lipid-related variants identified among Pakistanis corresponded to genes previously shown to be relevant to Europeans, such as CETP associated with HDL-C levels (rs711752; P<10(-13)), APOA5/ZNF259 (rs651821; P<10(-13)) and GCKR (rs1260326; P<10(-13)) with triglyceride levels; and CELSR2 variants with LDL-C levels (rs646776; P<10(-9)). For Pakistanis, these 41 variants explained 6.2%, 7.1%, and 0.9% of the variation in HDL-C, triglyceride, and LDL-C, respectively. Compared with Europeans, the allele frequency of rs662799 in APOA5 among Pakistanis was higher and its impact on triglyceride concentration was greater (P-value for difference <10(-4)).

Conclusions: Several lipid-related genetic variants are common to Pakistanis and Europeans, though they explain only a modest proportion of population variation in lipid concentration. Allelic frequencies and effect sizes of lipid-related variants can differ between Pakistanis and Europeans.
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http://dx.doi.org/10.1161/CIRCGENETICS.109.906180DOI Listing
August 2010

Genome-wide association study of CNVs in 16,000 cases of eight common diseases and 3,000 shared controls.

Nature 2010 Apr;464(7289):713-20

Copy number variants (CNVs) account for a major proportion of human genetic polymorphism and have been predicted to have an important role in genetic susceptibility to common disease. To address this we undertook a large, direct genome-wide study of association between CNVs and eight common human diseases. Using a purpose-designed array we typed approximately 19,000 individuals into distinct copy-number classes at 3,432 polymorphic CNVs, including an estimated approximately 50% of all common CNVs larger than 500 base pairs. We identified several biological artefacts that lead to false-positive associations, including systematic CNV differences between DNAs derived from blood and cell lines. Association testing and follow-up replication analyses confirmed three loci where CNVs were associated with disease-IRGM for Crohn's disease, HLA for Crohn's disease, rheumatoid arthritis and type 1 diabetes, and TSPAN8 for type 2 diabetes-although in each case the locus had previously been identified in single nucleotide polymorphism (SNP)-based studies, reflecting our observation that most common CNVs that are well-typed on our array are well tagged by SNPs and so have been indirectly explored through SNP studies. We conclude that common CNVs that can be typed on existing platforms are unlikely to contribute greatly to the genetic basis of common human diseases.
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http://dx.doi.org/10.1038/nature08979DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2892339PMC
April 2010

[Validation of the German version of the Australian Scale of Asperger's Syndrome (ASAS)].

Z Kinder Jugendpsychiatr Psychother 2005 Jan;33(1):27-34

Klinik und Poliklinik für Kinder- und Jugendpsychiatrie und Psychotherapie, Universität Würzburg.

Objectives: The aim of the study was to validate the German version of the Australian Scale for Asperger's Syndrome (ASAS). Furthermore, the scoring of the ASAS as applied by the Australian authors was verified.

Methods: The mothers of 18 children with Asperger's Syndrome, those of 18 children referred for a possible diagnosis of Asperger's Syndrome, but who did not receive that diagnosis, and the mothers of 15 children with other mental disorders participated in the study. All of the children were inpatients at the University of Wuerzburg Hospital of Child and Adolescent Psychiatry.

Results: According to an analysis of variance, the scale successfully differentiates among the three samples. A stepwise discriminant analysis was performed. Classification results show that the membership of the three groups could be labelled accurately (accuracy rate: 60.78%). The ASAS's scoring methodology appears to yield good results for German patients.

Conclusions: The scale appears to be an adequate tool for screening purposes in that it correctly discriminates children and adolescents with Asperger's Syndrome.
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http://dx.doi.org/10.1024/1422-4917.33.1.27DOI Listing
January 2005

LIMaS: the JAVA-based application and database for microarray experiment tracking.

Mamm Genome 2004 Sep;15(9):740-7

CEH-Oxford, Mansfield Road, OX1 3SR, Oxford, Oxfordshire, UK.

Microarrays allow monitoring of gene expression for tens of thousands of genes in parallel and are being used routinely to generate huge amounts of valuable data. Handling and analysis of such data are becoming major bottlenecks in the utilization of the technology. To enable the researcher to interpret the results postanalysis, we have developed a laboratory information management system for microarrays (LIMaS) with an n-tier Java front-end and relational database to record and manage large-scale expression data preanalysis. This system enables the laboratory to replace the paper trail with an efficient and fully customizable interface giving it the ability to adapt to any working practice, e.g., handling many resources used to form many products (chaining of resources). The ability to define sets of activities, resources, and workflows makes LIMaS MIAME-supportive.
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http://dx.doi.org/10.1007/s00335-004-2357-5DOI Listing
September 2004