Anoop Kumar

Dr. Anoop Kumar

PhD

ISF College of Pharmacy, Moga

Ranchi, Jharkhand | India

Specialties: Immunotoxicology, clinical research

Anoop Kumar

Dr. Anoop Kumar

PhD
Introduction

Dr. Anoop Kumar, currently working as Associate Professor in Department of Pharmacology, Indo-Soviet College of Pharmacy (ISFCP), Moga, Punjab.

Primary Affiliation: ISF College of Pharmacy, Moga - Ranchi, Jharkhand , India

Specialties:

Research Interests:


View Dr. Anoop Kumar’s Resume / CV
Metrics

17

Publications

1214

Profile Views

93

Reads

11

PubMed Central Citations

Top co-authors
Neelima Sharma
Neelima Sharma

Birla Institute of Technology

7
Kunal Mukhopadhyay
Kunal Mukhopadhyay

Birla Institute of Technology

2
Surender Singh Jadav
Surender Singh Jadav

Indian Institute of Chemical Technology

2
Amand Bhaskar
Amand Bhaskar

Birla Institute of Technology

2
Dinakar Sasmal
Dinakar Sasmal

Birla Institute of Technology

2
Venkatesan Jayaprakash
Venkatesan Jayaprakash

Birla Institute of Technology

1
Swastika Ganguly
Swastika Ganguly

Birla Institute of Technology

1
Aman Thakur
Aman Thakur

Institute of Pharmaceutical Sciences

1
Mohamed Jawed Ahsan
Mohamed Jawed Ahsan

Alwar Pharmacy College

1
Raju Poddar
Raju Poddar

Birla Institute of Technology

1

Publications

17Publications

93Reads

11PubMed Central Citations

Risks Associated with SGLT2 Inhibitors: An Overview.

Curr Drug Saf 2018 ;13(2):84-91

Department of Pharmacology, Indo-Soviet Friendship Pharmacy College (ISFCP), Moga, Punjab, India.

View Article
January 2018
9 Reads

Homology model, molecular dynamics simulation and novel pyrazole analogs design of Candida albicans CYP450 lanosterol 14 α-demethylase, a target enzyme for antifungal therapy.

J Biomol Struct Dyn 2017 May 2;35(7):1446-1463. Epub 2016 Aug 2.

a Department of Pharmaceutical Sciences and Technology , Birla Institute of Technology, Mesra , Ranchi , Jharkhand 835215 , India.

View Article
May 2017
11 Reads
2.98 Impact Factor

Ebola Virus Altered Innate and Adaptive Immune Response Signalling Pathways: Implications for Novel Therapeutic Approaches.

Authors:
Anoop Kumar

Infect Disord Drug Targets 2016 ;16(2):79-94

Department of Pharmaceutical Sciences and Technology, Birla Institute of Technology (B.I.T), Mesra, Ranchi-835215, Jharkhand, India.

View Article
March 2017
8 Reads
2 PubMed Central Citations(source)

Mechanism of immunoprotective effects of curcumin in DLM-induced thymic apoptosis and altered immune function: an in silico and in vitro study.

Immunopharmacol Immunotoxicol 2015 15;37(6):488-98. Epub 2015 Oct 15.

a Department of Pharmaceutical Sciences and Technology , Birla Institute of Technology , Ranchi , Jharkhand , India.

View Article
September 2016
11 Reads
1.11 Impact Factor

Deltamethrin-induced oxidative stress and mitochondrial caspase-dependent signaling pathways in murine splenocytes.

Environ Toxicol 2016 Jul 23;31(7):808-19. Epub 2014 Dec 23.

Department of Pharmaceutical Sciences and Technology, Birla Institute of Technology, Mesra, Ranchi-835215, Jharkhand, India.

View Article
July 2016
8 Reads
3.20 Impact Factor

Ebola virus: current and future perspectives.

Infect Disord Drug Targets 2015 ;15(1):20-31

Department of Pharmaceutical Sciences and Technology, Birla Institute of Technology, Mesra, Ranchi- 835215, Jharkhand, India.

View Article
January 2016
6 Reads
1 PubMed Central Citation(source)

Deltamethrin, a pyrethroid insecticide, could be a promising candidate as an anticancer agent.

Med Hypotheses 2015 Aug 1;85(2):145-7. Epub 2015 May 1.

Department of Bio-Engineering, Birla Institute of Technology, Mesra, Ranchi 835215, Jharkhand, India.

View Article
August 2015
7 Reads
1.15 Impact Factor

Determination of Deltamethrin in Mice Plasma and Immune Organs by Simple Reversed-Phase HPLC

Acta Chromatographica

Deltamethrin, a well-known type 2 synthetic pyrethroid insecticide, is a widespread environmental toxicant. It has potential to accumulate in body fluids and tissues due to its lipophilic characteristics. The immune system is among the most sensitive targets regarding toxicity of environmental pollutants. Various methods are available in the literature to analyze deltamethrin (DLM) concentration in plasma and tissues, but regarding the immune organs, only one gas chromatography-tandem mass spectrometry (GC-MS/MS) method (on spleen tissues) has been reported. In the present investigation, a rapid and sensitive high-performance liquid chromatography (HPLC) method has been developed and validated to determine DLM concentration in plasma, thymus, and spleen using zaleplone as an internal standard. Liquid chromatography (LC) separation is performed on an Agilent Zorbax® C8 column (250 mm × 4.6 mm, i.d., 5 µm) with isocratic elution using a mobile phase consisting of acetonitrile-5 mM KH2PO4 (70:30, v/v) at a flow rate of 1 mL min-1. The lower limit of quantification (LLOQ) for DLM is 10 ng mL-1 (plasma, thymus, and spleen). The method has been validated in terms of establishing linearity, specificity, sensitivity, recovery, accuracy, and precision (intra- and inter-day) and stabilities study. This validated method was successfully applied to a pharmacokinetic and tissue distribution study of DLM in mice.

View Article
August 2015
17 Reads

An insight into deltamethrin induced apoptotic calcium, p53 and oxidative stress signalling pathways

Toxicology and Environmental Health Sciences

Deltamethrin (DLM), a type 2 synthetic pyrethroid insecticide, is widely used in home and agricultural pest control. Humans are exposed to DLM by various modes like air, water, vegetables, etc. at low concentrations. DLM has been considered as safe for humans at low concentrations. At present, its exposure has been increased due to restriction on the sale of organophosphate insecticides. Increasing use of DLM products arise the attention towards its toxicity. It has been demonstrated that DLM at high concentration cause apoptosis in the mammalian cells. However, the mechanism of DLM mediated apoptotic signaling pathways are still an open question to be explored. In the present investigation, various pathways by which DLM induces chaos to different normal cellular activities have been compiled. Possibilities of DLM induced other apoptotic signalling pathways have also been discussed which could be considered for the future studies for better understanding of its mechanism of toxicity. This review provides the detailed study of all the existing and possible apoptogenic signalling pathways induced by DLM which could be helpful for the development of various antidotes for its toxicity.

View Article
April 2015
18 Reads

Immunomodulatory role of piperine in deltamethrin induced thymic apoptosis and altered immune functions.

Environ Toxicol Pharmacol 2015 Mar 8;39(2):504-14. Epub 2015 Jan 8.

Department of Pharmaceutical Science and Technology, Birla Institute of Technology, Mesra, Ranchi, 835215, Jharkhand, India. Electronic address:

View Article
March 2015
11 Reads
2 PubMed Central Citations(source)
1.86 Impact Factor

Comparative efficacy of piperine and curcumin in deltamethrin induced splenic apoptosis and altered immune functions.

Pestic Biochem Physiol 2015 Mar 10;119:16-27. Epub 2015 Mar 10.

Department of Pharmaceutical Sciences and Technology, Birla Institute of Technology, Mesra, Ranchi -835215, Jharkhand, India. Electronic address:

View Article
March 2015
9 Reads
2 PubMed Central Citations(source)
2.01 Impact Factor

Comparative efficacy of piperine and curcumin in deltamethrin induced splenic apoptosis and altered immune functions

doi:10.1016/j.pestbp.2015.03.003

Pesticide biochemistry and physiology

Deltamethrin (DLM) being a potent immunotoxicant affects both humoral and cell mediated immunity. Thus, for the amelioration of its effects, two different bioactive herbal extracts piperine and curcumin are evaluated and their efficacy has been compared. The docking results demonstrated that curcumin has good binding affinity towards CD28 and CD45 receptors as compared to piperine but in vitro studies revealed that piperine is more effective. DLM induced apoptotic markers such as oxidative stress and caspase 3 have been attenuated more significantly by piperine as compared to curcumin. Phenotypic and cytokine changes have also been mitigated best with piperine. Thus, these findings strongly demonstrate that piperine displays the more anti-oxidative, anti-apoptotic and chemo-protective properties in the DLM induced splenic apoptosis as compared to curcumin. So, piperine can be considered the drug of choice under immunocompromised conditions.

View Article
March 2015
14 Reads

Mechanism of Deltamethrin induced Immunotoxicity: Current and Future Perspectives

2015; 2: e578. doi: 10.14800/rci.578;

Receptors & Clinical Investigation

The immune system is the most vulnerable system regarding toxicity of pesticides. Any alteration in the immune functions makes an individual immunocompromised and more susceptible to cancer, infections, autoimmunity and allergies. Deltamethrin is the most popular type 2 pyrethroid insecticide which is widely use in agriculture and home due to restriction on the organophosphate insecticides. Due to their extensive use, it becomes an increasingly serious source of chemical pollution. We all are exposed to deltamethrin through inhalation, ingestion and dermal contact. It has been demonstrated that deltamethrin alters the immune response signalling pathways, but its mechanism of immunotoxicity is still an open question for researchers to be explored. Thus, herein we tried to understand the mechanism of deltamethrin induced immunotoxicity. Possibilities of deltamethrin induced other immunotoxic signalling pathways have also been discussed and should be considered in future studies. Further, current challenges and future perspectives have been also discussed.

View Article
February 2015
19 Reads

Deltamethrin induced an apoptogenic signalling pathway in murine thymocytes : exploring the molecular mechanism.

J Appl Toxicol 2014 Dec 12;34(12):1303-10. Epub 2013 Nov 12.

Department of Pharmaceutical Sciences, Birla Institute of Technology, Mesra, Ranchi, 835215, Jharkhand, India.

View Article
December 2014
7 Reads
3 PubMed Central Citations(source)
2.98 Impact Factor

Deltamethrin-Induced Oxidative Stress and Mitochondrial Caspase-Dependent Signaling Pathways in Murine Splenocytes

Environmental Toxicology

Deltamethrin (DLM) is a well-known pyrethroid insecticide used extensively in pest control. Exposure to DLM has been demonstrated to cause apoptosis in various cells. However, the immunotoxic effects of DLM on mammalian system and its mechanism is still an open question to be explored. To explore these effects, this study has been designed to first observe the interactions of DLM to immune cell receptors and its effects on the immune system. The docking score revealed that DLM has strong binding affinity toward the CD45 and CD28 receptors. In vitro study revealed that DLM induces apoptosis in murine splenocytes in a concentration-dependent manner. The earliest markers of apoptosis such as enhanced reactive oxygen species and caspase 3 activation are evident as early as 1 h by 25 and 50 mM DLM. Western blot analysis demonstrated that p38 MAP kinase and Bax expression is increased in a concentration-dependent manner, whereas Bcl 2 expression is significantly reduced after 3 h of DLM treatment. Glutathione depletion has been also observed at 3 and 6 h by 25 and 50 mM concentration of DLM. Flow cytometry results imply that the fraction of hypodiploid cells has gradually increased with all the concentrations of DLM at 18 h. N-acetyl cysteine effectively reduces the percentage of apoptotic cells, which is increased by DLM. In contrast, buthionine sulfoxamine causes an elevation in the percentage of apoptotic cells. Phenotyping data imply the effect of DLM toxicity in murine splenocytes. In brief, the study demonstrates that DLM causes apoptosis through its interaction with CD45 and CD28 receptors, leading to oxidative stress and activation of the mitochondrial caspase-dependent pathways which ultimately affects the immune functions. This study provides mechanistic information by which DLM causes toxicity in murine splenocytes.

View Article
December 2014
18 Reads

Mechanism of immunotoxicological effects of tributyltin chloride on murine thymocytes.

Cell Biol Toxicol 2014 Apr 27;30(2):101-12. Epub 2014 Feb 27.

Department of Pharmaceutical Sciences, Birla Institute of Technology, Mesra, Ranchi, 835215, Jharkhand, India,

View Article
April 2014
6 Reads
1 PubMed Central Citation(source)
2.68 Impact Factor

Oral Vaccine Antigen Induced Immune Response Signalling Pathways: Current and Future Perspectives

journal of vaccine and vaccination

Vaccination is the most promising approach to control and prevent the infectious diseases. As most of pathogenic microrganisms gain entry through the mucosal surfaces of host which provide great deal of interest in developing oral vaccines. Despite the success of many vaccines, only little information is available regarding the oral vaccine antigen induced immunogenic signalling pathways. Such information will be helpful to design future vaccines against old and new infectious diseases to reduce the side effects of existing vaccines and increase their efficacy. In this review, the oral vaccine antigen inducing complex signaling pathways of immune system has been discussed. Various strategies to prevent inactivation of oral vaccine by gastric acid and intestinal enzymes have been also included.

View Article
April 2014
15 Reads
Top co-authors
Neelima Sharma
Neelima Sharma

Birla Institute of Technology

7
Kunal Mukhopadhyay
Kunal Mukhopadhyay

Birla Institute of Technology

2
Surender Singh Jadav
Surender Singh Jadav

Indian Institute of Chemical Technology

2
Amand Bhaskar
Amand Bhaskar

Birla Institute of Technology

2
Dinakar Sasmal
Dinakar Sasmal

Birla Institute of Technology

2
Venkatesan Jayaprakash
Venkatesan Jayaprakash

Birla Institute of Technology

1
Swastika Ganguly
Swastika Ganguly

Birla Institute of Technology

1
Aman Thakur
Aman Thakur

Institute of Pharmaceutical Sciences

1
Mohamed Jawed Ahsan
Mohamed Jawed Ahsan

Alwar Pharmacy College

1
Raju Poddar
Raju Poddar

Birla Institute of Technology

1
Followers
Saket Chandra
Saket Chandra

Birla Institute of Technology