Publications by authors named "Anom Bowolaksono"

16 Publications

  • Page 1 of 1

Analysis of Bio-Risk Management System Implementation in Indonesian Higher Education Laboratory.

Int J Environ Res Public Health 2021 05 11;18(10). Epub 2021 May 11.

Disaster Risk Reduction Centre (DRRC), Universitas Indonesia, Depok 16424, Indonesia.

Developing countries face various challenges in implementing bio-risk management systems in the laboratory. In addition, educational settings are considered as workplaces with biohazard risks. Every activity in a laboratory facility carries many potential hazards that can impact human health and the environment and may cause laboratory incidents, including Laboratory Acquired Infections (LAIs). In an effort to minimize the impact and occurrence of these incidents, it is necessary to evaluate the implementation of a bio-risk management system in every activity that involves handling biological agents. This study was conducted in an Indonesian higher-education institution, herein coded as University Y. This is a descriptive, semi-quantitative study aimed at analysing and evaluating the implementation of the bio-risk management systems used in laboratories by analysing the achievements obtained by each laboratory. The study used primary data that were collected using a checklist which referred to ISO 35001:2019 on Laboratory Bio-risk Management. The checklist consisted of 202 items forming seven main elements. In addition, secondary data obtained from literature and document review were also used. The results show that out of 11 laboratories examined, only 2 laboratories met 50% of the requirements, which were Laboratory A and B, achieving good performance. Regarding the clauses of standards, a gap analysis identified leadership, performance evaluation, and support as elements with the lowest achievement. Therefore, corrective action should be developed by enhancing the commitment from management as well as improving documentation, policy, education and training.
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http://dx.doi.org/10.3390/ijerph18105076DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151126PMC
May 2021

[6]-Gingerol Inhibits Chikungunya Virus Infection by Suppressing Viral Replication.

Biomed Res Int 2021 27;2021:6623400. Epub 2021 Mar 27.

Eijkman Institute for Molecular Biology, Ministry of Research and Technology/National Agency for Research and Innovation, Jl. Diponegoro 69, Jakarta 10430, Indonesia.

Chikungunya (CHIK) is a reemerging arboviral disease caused by chikungunya virus (CHIKV) infection. The disease is clinically hallmarked by prolonged debilitating joint pain. Currently, there is no specific antiviral medication nor commercial vaccine available for treatment of the disease, which makes the discovery or development of specific anti-CHIKV compounds a priority. Ginger ( Roscoe) is widely known for its various health benefits. The compound [6]-gingerol is the main active ingredient found in ginger. This study sought to determine the potential of [6]-gingerol antiviral activity against CHIKV infection using human hepatocyte HepG2 cells. The antiviral activity mechanism was investigated using direct virucidal and four indirect (pre-, post-, full-, and prevention) treatment assays. [6]-Gingerol showed weak virucidal activity but significant indirect antiviral activity against CHIKV through post- and full treatment with IC of 0.038 mM and 0.031 mM, respectively, without showing cell cytotoxicity. The results indicated that [6]-gingerol inhibits CHIKV infection through suppression of viral replication. Together, this study confirms the potential use of [6]-gingerol for CHIK antiviral compound.
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http://dx.doi.org/10.1155/2021/6623400DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8019639PMC
May 2021

Implementation of Bio-Risk Management System in a National Clinical and Medical Referral Centre Laboratories.

Int J Environ Res Public Health 2021 02 26;18(5). Epub 2021 Feb 26.

Disaster Risk Reduction Centre (DRRC), Universitas Indonesia, Depok West Java 16424, Indonesia.

The increasing threats from biological agents have become a concern in laboratories, and emerging infectious diseases have demanded increased awareness and preparedness of laboratory facilities. Bio-risk assessment is needed to provide a framework for organisations to establish a comprehensive bio-risk management system. The assessment criteria should include both biosafety and biosecurity measures. Laboratories in Indonesia play a significant role in public health interventions in term of disease screening, diagnosis and medical decision making. The National Clinical and Medical Referral Centre Laboratories have the potential of daily exposures to dangerous biological materials. This study aims to identify the gap between bio-risk management system implementation and International Standard Organisation (ISO) 35001:2019 requirements. The 202 items in ISO 35001:2019 are categorized into seven main elements. The findings show that more than half of the elements on ISO 35001:2019 have been implemented in these centres. Good performance was identified at lab 4 and 5 which obtained the highest scores, particularly in the context of organisation, planning, operation and improvement elements. However, the widest gap was found in leadership, support and performance evaluation. One way to address this would be to create written rules and regulations at the laboratory top management level to require all laboratory facilities to comply to the bio-risk policies, rules, and regulations.
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http://dx.doi.org/10.3390/ijerph18052308DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7956505PMC
February 2021

The effects of luteinizing hormone as a suppression factor for apoptosis in bovine luteal cells in vitro.

Reprod Domest Anim 2021 May 22;56(5):744-753. Epub 2021 Feb 22.

Cellular and Molecular Mechanisms in Biological System (CEMBIOS) Research Group, Department of Biology, Faculty of Mathematics and Natural Sciences of Universitas Indonesia, Depok, Indonesia.

The fate of the corpus luteum, a transient endocrine gland formed and degraded during an oestrous cycle, is decided by various physiological factors, such as luteinizing hormone (LH). As a stimulator of progesterone, LH is known to maintain corpus luteum functional and structural integrity by inhibiting apoptosis, a programmed cell death. Therefore, we aim to investigate its action during the mid-luteal phase hypothesized that LH suppresses the death mechanism of bovine luteal steroidogenic cells (LSC) by analysing the expression of proteins involved. Cultured bovine LSC obtained from corpus luteum were treated for 24 hr with recombinant TNF and IFNG in the presence or absence of LH. The result showed that LH proved to have a protective effect by increased cell viability (p < .05) and prevented DNA fragmentation (p < .05), as demonstrated by the WST-1 colorimetric assay and TUNEL assay. Expression analysis of mRNA and protein levels showed that LH altered the expression of BCL2 (p < .05), CASP3 (p < .05), FAS (p < .05), and BAX (p < .05) to support cell survival. In conclusion, our study suggests that LH prolongs the corpus luteum life span through the anti-apoptotic mechanism by increasing cell viability and suppressing apoptosis-related genes and protein expression.
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http://dx.doi.org/10.1111/rda.13913DOI Listing
May 2021

The impact of late follicular progesterone level on in vitro fertilization-intracytoplasmic sperm injection outcome: Case-control study.

Int J Reprod Biomed 2020 May 31;18(5):367-374. Epub 2020 May 31.

Department of Biology, Faculty of Mathematics and Science, University of Indonesia, Depok, Indonesia.

Background: Studies have been conducted to improve the pregnancy rate through the in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) program. In recent years, researchers have been focusing on finding impact of high progesterone level on endometrial receptivity. However, data on whether progesterone level also affects the quality of the embryo is still limited.

Objective: The aim is to assess the effect of late follicular progesterone level on the outcome of in vitro fertilization-intracytoplasmic sperm injection (IVF-ICSI).

Materials And Methods: This was a case-control of 245 women who underwent in vitro fertilization cycle at Halim Fertility Center, Indonesia. The outcomes assessed were number of oocytes retrieved (OR), maturation rate (MR), fertilization rate (FR), number of good embryos (GE), number of fair embryos (FE), and number of poor embryos (PE). The progesterone (P4) and estradiol (E2) levels were analyzed on the day of human chorionic gonadotropin injection. Serum progesterone level was divided into three groups: 1. low progesterone ( 0.50 ng/ml), 2. normal progesterone (0.51-1.50 ng/ml), and 3. high progesterone ( 1.50 ng/ml). All outcomes were compared amongst the groups.

Results: Significant differences occurred between progesterone level on the day of human chorionic gonadotropin administration. The number of OR in group 1, 2, and 3 were 8.41 5.88 vs. 12.99 8.51 vs. 17.58 9.52, respectively.

Conclusion: Progesterone level on the day of human chorionic gonadotropin injection may have an impact on the outcome of IVF-ICSI.
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http://dx.doi.org/10.18502/ijrm.v13i5.7157DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7306063PMC
May 2020

Evaluation of the implementation of occupational health, safety, and environment management systems in higher education laboratories.

J Chem Health Saf 2019 Jul-Oct;26(4):14-19. Epub 2019 Feb 1.

Laboratory researchers and students may expose to hazardous and toxic chemicals. Implementation of the Occupational Health, Safety, and Environment Management System (OHSEMS) has become a critical aspect in higher education. This study presents an overview of the evaluation of the implementation of the OHSEMS in higher education laboratories. The implementation of the OHSEMS is to prevent occupational accidents in the laboratory. The study design is a semiquantitative descriptive study. The aim of the study is to evaluate the implementation of the OHSEMS in higher education institution laboratories by evaluating the percentages of OHSEMS compliance in higher education laboratories. Five aspects are evaluated: occupational health, safety, and environment (OHSE) policy and commitment, planning, implementation, evaluation, and management review. The result shows that the average compliance with the OHSE policy and commitment aspect is 59.4% and for the planning, implementation, evaluation, and management review, the average compliance percentage are 33.0%, 65.3%, 26.0%, and 0.0%, respectively.
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http://dx.doi.org/10.1016/j.jchas.2018.12.006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7102750PMC
February 2019

Identification and functional characterization of major facilitator superfamily (MFS) transporters.

Biosci Biotechnol Biochem 2020 Jul 12;84(7):1373-1383. Epub 2020 Mar 12.

School of Life Science and Technology, Tokyo Institute of Technology , Tokyo, Japan.

Penicillium Marneffei: is a thermally dimorphic fungus that causes penicilliosis, and become the third-most-common opportunistic fungal infection in immunocompromised patients in Southeast Asia. Azoles and amphotericin B have been introduced for the treatment, however, it is important to investigate possible mechanisms of azole resistance for future treatment failure. We identified 177 putative MFS transporters and classified into 17 subfamilies. Among those, members of the Drug:H antiporter 1 subfamily are known to confer resistance to antifungals. Out of 39 paralogs, three (encoded by , and ) were heterologously overexpressed in AD∆ conferred resistance to various drugs and compounds including azoles, albeit to different degrees. MDR1-expressing strain showed resistance to the broadest range of drugs, followed by the MDR3, and MDR2 conferred weak resistance to a limited range of drugs. We conclude that and , may be able to serve as multidrug efflux pumps.
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http://dx.doi.org/10.1080/09168451.2020.1732185DOI Listing
July 2020

Obesity in the Balinese is associated with FTO rs9939609 and rs1421085 single nucleotide polymorphisms.

PeerJ 2020 3;8:e8327. Epub 2020 Jan 3.

Eijkman Institute for Molecular Biology, Jakarta, Indonesia.

Obesity prevalence is increasing worldwide, including in the Bali Province, Indonesia, a popular tourism destination area. The common single nucleotide polymorphisms (SNPs) rs9939609 and rs1421085 of the fat mass and obesity-associated (FTO) gene have been repeatedly reported as one of the important obesity genetic risk factors. We have examined the associations of FTO rs9939609 and rs1421085 SNPs with obesity in the 612 unrelated Balinese subjects living in urban and rural areas. Linear and logistic regression analyses with adjustment for age and gender were employed to investigate the association between FTO genotypes, haplotypes and obesity parameters. We found that the FTO SNPs genotypes increased BMI by 1.25 kg/m ( = 0.012) for rs9939609 AA and 1.12 kg/m ( = 0.022) for rs1421085 CC, particularly in females and in rural population. Subjects carrying these genotypes also showed a tendency to maintain high BMI, regardless of their age. Our result showed that the FTO rs9939609 and rs1421085 risk alleles were associated with increased BMI and obesity in the Balinese.
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http://dx.doi.org/10.7717/peerj.8327DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6944099PMC
January 2020

Placental mitochondrial DNA copy number is associated with reduced birth weight in women with placental malaria.

Placenta 2019 05 15;80:1-3. Epub 2019 Mar 15.

Eijkman Institute for Molecular Biology, Jl. Diponegoro No. 69, Jakarta, 10430, Indonesia. Electronic address:

Placental malaria (PM) causes placental insufficiency, leading to reduced birth weight (BW). Placental mtDNA copy number (mtDNA-CN) and relative telomere length (RTL) have been described as potential biomarkers for placental insufficiency and intrauterine growth restriction (IUGR). We investigated their associations with BW in women with PM from malaria-endemic region of Papua, Indonesia. MtDNA-CN and RTL were determined in 50 placentas by quantitative real-time PCR. Increased placental mtDNA-CN was associated with reduced BW (coef = -193.71, p = 0.016), particularly in preterm group (coef = -374.21, p < 0.001). RTL did not associate with BW. Increased placental mtDNA-CN indicates a compensatory mechanism to reduced BW in women with PM.
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http://dx.doi.org/10.1016/j.placenta.2019.03.005DOI Listing
May 2019

Luteoprotective mechanisms of prostaglandin F2α stimulated by luteinizing hormone in the bovine corpus luteum.

J Reprod Dev 2013 25;59(3):225-30. Epub 2013 Jan 25.

Laboratory of Reproductive Physiology, Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan.

Luteinizing hormone (LH) regulates several ovarian functions. However, the luteoprotective mechanisms of LH involved in the maintenance of bovine corpus luteum (CL) function are not well understood. Since prostaglandin F2α (PGF), PGE2 and progesterone (P4) are well documented as antiapoptotic factors in the bovine CL, we hypothesized that LH protects the CL by stimulating the local production and action of PGF, PGE2 and P4. Cultured bovine luteal cells obtained at the mid-luteal stage (days 8-12 of the estrous cycle) were treated with LH (10 ng/ml), onapristone (OP: a specific P4 receptor antagonist, 100 μM) and indomethacin [INDO; a cyclooxygenase (COX) inhibitor, 100 μM] for 24 h. LH with and without OP significantly increased the mRNA and protein expressions of COX-2, PGF synthase and carbonyl reductase (P<0.05) but not the mRNA and protein expressions of COX-1 and PGE synthase in bovine luteal cells. In addition, these treatments significantly increased PGF and P4 production (P<0.05) but not PGE2 production. Luteal cell viability was significantly increased by LH alone (P<0.05), but LH-increased cell viability was reduced by LH in combination with INDO as well as OP (P<0.05). The overall results suggest that LH prevents luteal cell death by stimulating luteal PGF and P4 production and supports CL function during the luteal phase in cattle.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3934132PMC
http://dx.doi.org/10.1262/jrd.2012-187DOI Listing
February 2014

Luteoprotective roles of luteinizing hormone are mediated by not only progesterone production but also glucocorticoid conversion in bovine corpus luteum.

Mol Reprod Dev 2013 Mar 31;80(3):204-11. Epub 2013 Jan 31.

Laboratory of Reproductive Physiology, Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan.

Luteinizing hormone (LH) is known as a key regulator of corpus luteum (CL) function, but the luteoprotective mechanisms of LH in the maintenance of bovine CL function are not well understood. The current study investigated if LH increases cell viability and induces cortisol conversion, and if the luteoprotective action of LH is mediated by stimulating the local production and action of progesterone (P4) and/or cortisol. Cultured bovine luteal cells obtained at the mid-luteal stage (Days 8-12 of the estrous cycle) were treated for 24 hr with LH (10 ng/ml) with/without onapristone (OP, a specific P4 receptor antagonist; 100 µM), cortisone (1 µM), and aminoglutethimide (AGT, a specific inhibitor of cytochrome P450 side-chain cleavage; 100 µM). LH with and without OP significantly increased the mRNA and protein expressions of 11β-hydroxysteroid dehydrogenase (HSD11B) 1, but did not affect the mRNA or protein expression of HSD11B2. These treatments also significantly increased HSD11B1 activity. Cell viability was significantly increased by LH alone or by LH in combination with cortisone and OP. LH in combination with OP or AGT significantly decreased cell viability as compared to LH alone. The overall results suggest that LH stimulates not only P4 production but also HSD11B1 expression, thereby increasing the cortisol concentration in the bovine CL, and that LH prevents cell death through these survival pathways. LH may consequently support CL function during the luteal phase in cattle.
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http://dx.doi.org/10.1002/mrd.22150DOI Listing
March 2013

Ovarian steroids regulate prostaglandin secretion in the feline endometrium.

Anim Reprod Sci 2010 Jul 3;120(1-4):142-50. Epub 2010 Mar 3.

Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn 10-747, Poland.

Sex steroids, i.e. progesterone (P(4)) and 17beta-estradiol (E(2)), fluctuate during the feline estrous cycle and their alterations correspond to many events in cat reproduction. In order to investigate possible effects of sex steroids on prostaglandin (PG) secretion in the cultured endometrial cells, mRNA expressions coding for PG-endoperoxide synthase (PTGS2) in the epithelial and stromal cells harvested with sex steroids were studied by RT-PCR. The effects of ovarian steroids on PG secretion in the epithelial and stromal cells were also investigated. E(2) at a dose 10(-7)M significantly increased prostaglandin F2alpha (PGF(2alpha)) secretion in the epithelial cells (P<0.01). PGF(2alpha) production was intensified by the treatment in combination with both steroids (P<0.001). P(4) at any dose alone had no effect on PGF(2alpha) secretion in the epithelial cells, whereas at a dose 10(-5)M enhanced prostaglandin E2 (PGE(2)) production (P<0.05). The ovarian steroids stimulated both PGF(2alpha) and PGE(2) in the epithelial cells of the feline endometrium via an E(2) receptor (ESR1)- and P(4) receptor (PGR)-dependent genomic-pathway. In contrast to the epithelial cells, neither P(4) nor E(2) affected PG secretion in the stromal cells. PTGS2 mRNA expression was not affected by ovarian steroids in either cell types. The overall results suggest that PG secretion is regulated by P(4) and E(2) and this effect is not due to changes in PTGS2 mRNA expression.
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http://dx.doi.org/10.1016/j.anireprosci.2010.02.020DOI Listing
July 2010

Survival role of locally produced acetylcholine in the bovine corpus luteum.

Biol Reprod 2009 Apr 7;80(4):823-32. Epub 2009 Jan 7.

Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan.

The present study was conducted to explore the source of acetylcholine (ACH) in the corpus luteum (CL) and to test our hypothesis of an antiapoptotic role of ACH in the bovine CL and, further, to investigate whether nerve growth factor (NGF), insulin-like growth factor 1 (IGF1), and transforming growth factor beta1 (TGFB1) influence the expression of choline acetyltransferase (CHAT), the biosynthetic enzyme of ACH, in cultured bovine luteal cells. Protein expression and immunolocalization of CHAT were carried out at different stages throughout the luteal phase and in cultured luteal and endothelial cells. ACH was measured in luteal tissue at the different luteal stages and in luteal cells cultured for 8 and 24 h. Cell viability and TUNEL assays were performed on cultured midluteal cells treated with or without tumor necrosis factor alpha (TNF)/interferon gamma (IFNG) in the presence of ACH and its muscarinic (atropine) and nicotinic (mecamylamine) receptor antagonists. The CL was devoid of cholinergic nerve fibers. CHAT immunostaining was evident in luteal, endothelial, and stromal cells in luteal tissue sections and in cultured luteal and endothelial cells. CHAT protein was expressed throughout the cycle without any significant changes. ACH concentration in luteal tissue was not changed during the luteal stages but increased over time and with increased cell numbers in luteal cell cultures. ACH increased cell viability and prevented cell death induced by TNF/IFNG. Atropine significantly attenuated ACH action, whereas mecamylamine had no effect. TNF/IFNG treatment downregulated CHAT expression, whereas NGF, IGF1, and TGFB1 upregulated CHAT expression, in cultured luteal cells. The overall findings strongly suggest a nonneural source and antiapoptotic role of ACH in the bovine CL. Locally produced ACH appears to be regulated by NGF, IGF1, and TGFB1.
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http://dx.doi.org/10.1095/biolreprod.108.069203DOI Listing
April 2009

Anti-apoptotic roles of prostaglandin E2 and F2alpha in bovine luteal steroidogenic cells.

Biol Reprod 2008 Aug 7;79(2):310-7. Epub 2008 May 7.

Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan.

Production of prostaglandins (PGs) and expression of their receptors have been demonstrated in bovine corpus luteum (CL). The aim of the present study was to determine whether PGE2 and PGF2alpha have roles in bovine luteal steroidogenic cell (LSC) apoptosis. Cultured bovine LSCs obtained at the midluteal stage (Days 8-12 of the cycle) were treated for 24 h with PGE2 (0.001-1 microM) and PGF2alpha (0.001-1 microM). Prostaglandin E2 (1 microM) and PGF2alpha (1 microM) significantly stimulated progesterone (P4) production and reduced the levels of cell death in the cells cultured with or without tumor necrosis factor alpha (TNF)/interferon gamma (IFNG), in the presence and absence of FAS ligand (P < 0.05). Furthermore, DNA fragmentation induced by TNF/IFNG was observed to be suppressed by PGE2 and PGF2alpha. Prostaglandin E2 and PGF2alpha also attenuated mRNA expression of caspase 3 and caspase 8, as well as caspase 3 activity (P < 0.05) in TNF/IFNG-treated cells. FAS mRNA and protein expression were decreased only by PGF2alpha (P < 0.05). A specific P4 receptor antagonist (onapristone) attenuated the apoptosis-inhibitory effects of PGE2 and PGF2alpha in the absence of TNF/IFNG (P < 0.05). A PG synthesis inhibitor (indomethacin) reduced cell viability in PGE2- and PGF2alpha-treated cells (P < 0.05). A specific inhibitor of cyclooxygenase (PTGS), PTGS2 (NS-398), also reduced cell viability, whereas an inhibitor of PTGS1 (FR122047) did not affect it. The overall results suggest that PGE2 and PGF2alpha locally play luteoprotective roles in bovine CL by suppressing apoptosis of LSCs.
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http://dx.doi.org/10.1095/biolreprod.107.066084DOI Listing
August 2008

Possible roles of intracellular cyclic AMP, protein kinase C and calcium ion in the apoptotic signaling pathway in bovine luteal cells.

J Reprod Dev 2006 Aug 8;52(4):517-22. Epub 2006 May 8.

Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University, Japan.

Structural luteolysis occurs by apoptosis of luteal cells. The present study examined the effects of activators of well-characterized second messengers on Fas and caspase-3 mRNA expression and on P4 production in luteal cells in order to trace the pro- and anti-apoptotic factors in the bovine corpus luteum (CL). Cultured bovine mid luteal cells were treated for 24 h with a cyclic AMP analogue (8-bromo cyclic AMP; 8br-cAMP; 2.5 mM), a protein kinase C (PKC) activator (phorbol 12-myristate 13-acetate; PMA; 10 microM), or calcium ionophore (A23187; 10 microM). Fas and caspase-3 mRNA expression was inhibited by 8br-cAMP and PMA but was increased by A23187 (P<0.05). In addition, P4 production by bovine luteal cells was stimulated by 8br-cAMP and PMA, whereas it was inhibited by A23187, compared with untreated controls (P<0.05). The overall results suggest that cAMP and PKC suppress apoptosis in bovine luteal cells through inhibition of Fas and caspase-3 mRNA expression and through stimulation of P4 production. Therefore, substances that activate cAMP or PKC may act as survival factors in the bovine CL. Furthermore, substances that mobilize Ca2+ may act as apoptotic factors by stimulating Fas and caspase-3 expression in the bovine luteal cells.
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http://dx.doi.org/10.1262/jrd.18024DOI Listing
August 2006

Possible role of interleukin-1 in the regulation of bovine corpus luteum throughout the luteal phase.

Biol Reprod 2004 Nov 14;71(5):1688-93. Epub 2004 Jul 14.

Laboratory of Reproductive Endocrinology, Okayama University, Okayama 700-8530, Japan.

Interleukin-1 (IL-1) is one of the principal cytokines that participate in local regulation of many reproductive functions. The present study was undertaken to determine whether mRNAs for IL-1alpha, IL-1beta, and IL-1 type I receptor (IL-1R) are expressed in bovine corpora lutea (CL), and whether luteal cells respond to treatment with IL-1alpha and IL-1beta during the luteal phase. Bovine CL were classified into five stages (early, Days 2-3; developing, Days 5-6; mid, Days 8-12; late, Days 15-17; and regressed, Days 19-21). IL-1alpha, IL-1beta, and IL-1R mRNAs were detected by reverse transcription-polymerase chain reaction (PCR) in all luteal stages examined. Densitometric analysis of PCR products revealed increases of the mRNA of IL-1alpha and IL-1R in the CL of the regressed stage (P < 0.05). There was less mRNA for IL-1beta in the regressed stage than in the developing and mid stages (P < 0.05). When developing, mid, and late luteal cells were treated with IL-1alpha (1-30 ng/ml) or IL-1beta (1-30 ng/ml) for 24 h, IL-1alpha and IL-1beta dose-dependently increased prostaglandin (PG) F(2alpha) and PGE(2) production by the luteal cells of all stages (P < 0.05), indicating the presence of functional IL-1R in bovine CL. However, progesterone synthesis was not affected by either IL-1alpha or IL-1beta treatment. Stimulation with IL-1alpha and IL-1beta decreased the PGE(2):PGF(2alpha) ratio in the developing stage (P < 0.05), whereas it increased the ratio in the mid stage (P < 0.05). In the late stage, the ratio of IL-1beta-treated cells was greater than that of IL-1alpha-treated cells (P < 0.05). Overall results indicate that genes for IL-1alpha and IL-1beta are expressed and a functional IL-1R is present in the bovine CL throughout the luteal phase, and suggest that IL-1alpha and IL-1beta have different roles as local modulators to regulate PGF(2alpha) and PGE(2) production during the luteal phase.
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http://dx.doi.org/10.1095/biolreprod.104.032151DOI Listing
November 2004
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