Publications by authors named "Anna Stępien"

40 Publications

Common gene variants within 3'-untranslated regions as modulators of multiple myeloma risk and survival.

Int J Cancer 2021 Apr 20;148(8):1887-1894. Epub 2020 Nov 20.

Department of Biology, University of Pisa, Pisa, Italy.

We evaluated the association between germline genetic variants located within the 3'-untranlsated region (polymorphic 3'UTR, ie, p3UTR) of candidate genes involved in multiple myeloma (MM). We performed a case-control study within the International Multiple Myeloma rESEarch (IMMEnSE) consortium, consisting of 3056 MM patients and 1960 controls recruited from eight countries. We selected p3UTR of six genes known to act in different pathways relevant in MM pathogenesis, namely KRAS (rs12587 and rs7973623), VEGFA (rs10434), SPP1 (rs1126772), IRF4 (rs12211228) and IL10 (rs3024496). We found that IL10-rs3024496 was associated with increased risk of developing MM and with a worse overall survival of MM patients. The variant allele was assayed in a vector expressing eGFP chimerized with the IL10 3'-UTR and it was found functionally active following transfection in human myeloma cells. In this experiment, the A-allele caused a lower expression of the reporter gene and this was also in agreement with the in vivo expression of mRNA measured in whole blood as reported in the GTEx portal. Overall, these data are suggestive of an effect of the IL10-rs3024496 SNP on the regulation of IL10 mRNA expression and it could have clinical implications for better characterization of MM patients in terms of prognosis.
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http://dx.doi.org/10.1002/ijc.33377DOI Listing
April 2021

Influence of Replacement Part of Starch with Inulin on the Rheological Properties of Pastes and Gels Based on Potato Starch.

Int J Food Sci 2020 26;2020:7642041. Epub 2020 Aug 26.

Department of Engineering and Machinery for Food Industry, University of Agriculture in Krakow, Balicka 122 Str., 30-149 Kraków, Poland.

The objective of the present study was to determine the influence of replacement part of starch with inulin on the rheological characteristics of pastes and gels obtained on the basis of potato starch. Replacement of the starch by inulin varied from 0 to 40%. Flow curves for pastes and gels were determined, and the viscoelastic properties were characterized using dynamic tests and creep and recovery tests. It was determined that the replacement of part of potato starch with inulin significantly modifies rheological properties of starch pastes and gels, weakening their structure. With the increasing amount of inulin, an increase of viscous properties was becoming more apparent. Moreover, an irregular influence of inulin addition on the parameters of rheological characteristics was determined. Initially, the differences were minor, and the differences at the lowest addition were typically statistically insignificant, followed by strong increase with local restrictions to structural weakening.
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http://dx.doi.org/10.1155/2020/7642041DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7474758PMC
August 2020

Undirected singing rate as a non-invasive tool for welfare monitoring in isolated male zebra finches.

PLoS One 2020 10;15(8):e0236333. Epub 2020 Aug 10.

Institute of Neuroinformatics and Neuroscience Center Zurich, University of Zurich and ETH Zurich, Zurich, Switzerland.

Research on the songbird zebra finch (Taeniopygia guttata) has advanced our behavioral, hormonal, neuronal, and genetic understanding of vocal learning. However, little is known about the impact of typical experimental manipulations on the welfare of these birds. Here we explore whether the undirected singing rate can be used as an indicator of welfare. We tested this idea by performing a post hoc analysis of singing behavior in isolated male zebra finches subjected to interactive white noise, to surgery, or to tethering. We find that the latter two experimental manipulations transiently but reliably decreased singing rates. By contraposition, we infer that a high-sustained singing rate is suggestive of successful coping or improved welfare in these experiments. Our analysis across more than 300 days of song data suggests that a singing rate above a threshold of several hundred song motifs per day implies an absence of an acute stressor or a successful coping with stress. Because singing rate can be measured in a completely automatic fashion, its observation can help to reduce experimenter bias in welfare monitoring. Because singing rate measurements are non-invasive, we expect this study to contribute to the refinement of the current welfare monitoring tools in zebra finches.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0236333PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7416931PMC
October 2020

The influence of the extrusion process on the nutritional composition, physical properties and storage stability of black chokeberry pomaces.

Food Chem 2021 Jan 17;334:127548. Epub 2020 Jul 17.

Department of Food Storage and Technology, Wroclaw University of Environmental and Life Science, Chełmońskiego 37/41 Str., 51-630 Wrocław, Poland. Electronic address:

The study examined the influence of the process of extrusion on the physical properties and nutritional composition of black chokeberry pomaces. It has been determined that the extrusion process resulted in a reduction of the content of anthocyanins and fibre, but an increase of the contribution of simple sugars. In order to assess the phase transitions occurring in the products, a state diagram was utilized, which was constructed using the freezing and vitrification curve and values characterizing the conditions of maximum cryoconcentration. The determined values of critical water activity (based on water activity concepts) indicate that pomaces and extrudates retain crispiness in storage under moderate environmental relative humidity conditions. However, in the case of the glass transition concept, the determined values of water activity indicate that products stored in room temperature must be protected against the influence of humidity.
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http://dx.doi.org/10.1016/j.foodchem.2020.127548DOI Listing
January 2021

Moisture sorption characteristics of food powders containing freeze dried avocado, maltodextrin and inulin.

Int J Biol Macromol 2020 Apr 20;149:256-261. Epub 2020 Jan 20.

Department of Engineering and Machinery for Food Industry, Faculty of Food Technology, University of Agriculture, Balicka 122 Street, 30-149 Krakow, Poland. Electronic address:

Moisture adsorption isotherms of the freeze-dried powders containing avocado, maltodextrin and inulin with different ratio, were determined using gravimetric static method of saturated salt solutions at 25 °C and the range of the water activity from 0.11 to 0.86. The data obtained were evaluated using sorption models: BET (Brunauer-Emmett-Teller), GAB (Guggenheim, Anderson and deBoer), Peleg, Lewicki, Oswin and Henderson. The goodness of the fit evaluated on the basis of criteria such as coefficient of the determination and root mean square values. Sorption isotherm of the pure avocado powder was III type while curves plotted for others powders showed sigmoid shape. It was found that the Peleg model was the most adequate for representation of the sorption data of all analyzed blends. In order to characterize the research material, chemical composition of the edible part of the avocado fruit was determined. Main component of the dry matter was fat.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.01.154DOI Listing
April 2020

Author Correction: Risk factors for serious morbidity, prolonged length of stay and hospital readmission after laparoscopic appendectomy - results from Pol-LA (Polish Laparoscopic Appendectomy) multicenter large cohort study.

Sci Rep 2019 Dec 6;9(1):18479. Epub 2019 Dec 6.

2nd Department of General Surgery, Jagiellonian University Medical College, Krakow, Poland.

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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http://dx.doi.org/10.1038/s41598-019-54993-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6897885PMC
December 2019

Cereblon () gene polymorphisms predict clinical response and progression-free survival in relapsed/refractory multiple myeloma patients treated with lenalidomide: a pharmacogenetic study from the IMMEnSE consortium.

Leuk Lymphoma 2020 03 20;61(3):699-706. Epub 2019 Nov 20.

Department of Hematology, Institute of Hematology and Transfusion Medicine, Warsaw, Poland.

Cereblon (CRBN) is crucial for antiproliferative and immunomodulatory properties of immunomodulatory drugs. The objective of this study was to verify whether germline single nucleotide polymorphisms (SNPs) in the gene may influence response to lenalidomide in multiple myeloma (MM). Fourteen tagging SNPs covering the genetic variability in the gene region were genotyped in 167 Polish patients with refractory/relapsed MM treated with lenalidomide-based regimens. We found that carriers of minor alleles of two studied CRBN SNPs rs1714327G > C (OR = 0.26; 95% CI = 0.1-0.67;  = .0055, Bonferroni corrected  = .033) and rs1705814T > C (OR = 0.22; 95% CI = 0.07-0.65;  = .0063, Bonferroni corrected  = .037) were significantly associated with lower probability of achievement at least partial remission while treated with lenalidomide-based regimens, using the dominant inheritance model. Moreover, one of these SNPs, namely rs1705814T > C, was correlated with shorter progression-free survival (HR = 2.49; 95%CI = 1.31-4.74,  = .0054, Bonferroni corrected  = .033). It is suggested that selected germline CRBN allelic variants (rs1714327G > C and rs1705814T > C) affect lenalidomide efficacy in patients with relapsed/refractory MM.
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http://dx.doi.org/10.1080/10428194.2019.1689391DOI Listing
March 2020

Risk factors for serious morbidity, prolonged length of stay and hospital readmission after laparoscopic appendectomy - results from Pol-LA (Polish Laparoscopic Appendectomy) multicenter large cohort study.

Sci Rep 2019 10 15;9(1):14793. Epub 2019 Oct 15.

2nd Department of General Surgery, Jagiellonian University Medical College, Krakow, Poland.

Laparoscopic appendectomy (LA) for treatment of acute appendicitis has gained acceptance with its considerable benefits over open appendectomy. LA, however, can involve some adverse outcomes: morbidity, prolonged length of hospital stay (LOS) and hospital readmission. Identification of predictive factors may help to identify and tailor treatment for patients with higher risk of these adverse events. Our aim was to identify risk factors for serious morbidity, prolonged LOS and hospital readmission after LA. A database compiled information of patients admitted for acute appendicitis from eighteen Polish and German surgical centers. It included factors related to the patient characteristics, peri- and postoperative period. Univariate and multivariate logistic regression models were used to identify risk factors for serious perioperative complications, prolonged LOS, and hospital readmissions in acute appendicitis cases. 4618 laparoscopic appendectomy patients were included. First, although several risk factors for serious perioperative complications (C-D III-V) were found in the univariate analysis, in the multivariate model only the presence of intraoperative adverse events (OR 4.09, 95% CI 1.32-12.65, p = 0.014) and complicated appendicitis (OR 3.63, 95% CI 1.74-7.61, p = 0.001) was statistically significant. Second, prolonged LOS was associated with the presence of complicated appendicitis (OR 2.8, 95% CI: 1.53-5.12, p = 0.001), postoperative morbidity (OR 5.01, 95% CI: 2.33-10.75, p < 0.001), conversions (OR 6.48, 95% CI: 3.48-12.08, p < 0.001) and reinterventions after primary procedure (OR 8.79, 95% CI: 3.2-24.14, p < 0.001) in the multivariate model. Third, although several risk factors for hospital readmissions were found in univariate analysis, in the multivariate model only the presence of postoperative complications (OR 10.33, 95% CI: 4.27-25.00), reintervention after primary procedure (OR 5.62, 95% CI: 2.17-14.54), and LA performed by resident (OR 1.96, 95% CI: 1.03-3.70) remained significant. Laparoscopic appendectomy is a safe procedure associated with low rates of complications, prolonged LOS, and readmissions. Risk factors for these adverse events include complicated appendicitis, postoperative morbidity, conversion, and re-intervention after the primary procedure. Any occurrence of these factors during treatment should alert the healthcare team to identify the patients that require more customized treatment to minimize the risk for adverse outcomes.
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http://dx.doi.org/10.1038/s41598-019-51172-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6794313PMC
October 2019

Corrigendum to "Thermodynamic properties and glass transition temperature of roasted and unroasted carob (Ceratonia silique L.) powder" [Food Chem. 300 (2019), 125208].

Food Chem 2020 Feb 4;305:125449. Epub 2019 Sep 4.

Department of Engineering and Machinery for Food Industry, Faculty of Food Technology, University of Agriculture, Balicka 122 Street., 30-149 Krakow, Poland.

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http://dx.doi.org/10.1016/j.foodchem.2019.125449DOI Listing
February 2020

Thermodynamic properties and glass transition temperature of roasted and unroasted carob (Ceratonia siliqua L.) powder.

Food Chem 2019 12 19;300:125208. Epub 2019 Jul 19.

Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 53210 Pardubice, Czech Republic. Electronic address:

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http://dx.doi.org/10.1016/j.foodchem.2019.125208DOI Listing
December 2019

The significant impact of age on the clinical outcomes of laparoscopic appendectomy: Results from the Polish Laparoscopic Appendectomy multicenter large cohort study.

Medicine (Baltimore) 2018 Dec;97(50):e13621

Faculty of Health Sciences, Powiślańska School in Kwidzyn, Kwidzyn.

Acute appendicitis (AA) is the most common surgical emergency and can occur at any age. Nearly all of the studies comparing outcomes of appendectomy between younger and older patients set cut-off point at 65 years. In this multicenter observational study, we aimed to compare laparoscopic appendectomy for AA in various groups of patients with particular interest in the elderly and very elderly in comparison to younger adults.Our multicenter observational study of 18 surgical units assessed the outcomes of 4618 laparoscopic appendectomies for AA. Patients were divided in 4 groups according to their age: Group 1-<40 years old; Group 2-between 40 and 64 years old; Group 3-between 65 and 74 years old; and Group 4-75 years old or older. Groups were compared in terms of peri- and postoperative outcomes.The ratio of complicated appendicitis grew with age (20.97% vs 37.50% vs 43.97% vs 56.84%, P < .001). Similarly, elderly patients more frequently suffered from perioperative complications (5.06% vs 9.3% vs 10.88% vs 13.68%, P < .001) and had the longest median length of stay (3 [Interquartile Range (IQR) 2-4] vs 3 [IQR 3-5], vs 4 [IQR 3-5], vs 5 [IQR 3-6], P < .001) as well as the rate of patients with prolonged length of hospital stay (LOS) >8 days. Logistic regression models comparing perioperative results of each of the 3 oldest groups compared with the youngest one showed significant differences in odds ratios of symptoms lasting >48 hours, presence of complicated appendicitis, perioperative morbidity, conversion rate, prolonged LOS (>8 days).The findings of this study confirm that the outcomes of laparoscopic approach to AA in different age groups are not the same regarding outcomes and the clinical picture. Older patients are at high risk both in the preoperative, intraoperative, and postoperative period. The differences are visible already at the age of 40 years old. Since delayed diagnosis and postponed surgery result in the development of complicated appendicitis, more effort should be placed in improving treatment patterns for the elderly and their clinical outcome.
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http://dx.doi.org/10.1097/MD.0000000000013621DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6320074PMC
December 2018

Gestational diabetes mellitus (GDM) - do the number of fulfilled diagnostic criteria predict the perinatal outcome?

Ginekol Pol 2018 ;89(7):381-387

Second Department of Obstetrics and Gynecology, Medical University of Warsaw, Karowa 2, 00-315 Warsaw, Poland.

Objectives: The aim of the study was to check whether the number of fulfilled diagnostic criteria of gestational diabetes mellitus (GDM) had any association with patients' characteristics and pregnancy outcomes.

Material And Methods: A total of 756 women with single pregnancies and GDM who gave birth at the 2nd Department of Obstetrics and Gynecology of the Medical University of Warsaw between 01.2013-12.2016 were included in a retrospective analysis. Patients were divided into 2 groups: A - 499 patients diagnosed with GDM on the basis of one diagnostic criterion, B - 257 patients diagnosed with GDM on the basis of more than one diagnostic criterion.

Results: Patients from group A had lower pre-pregnancy BMI than those from group B (median 24.9 kg/m2 vs. 26.5 kg/m2, p=0.0003). Women from group A were less frequently treated with insulin than women from group B (19.1% vs. 32.7%; p=0.00002). Group A had lower median OGTT levels than group B (85.9 mg/dL vs. 94.1 mg/dL, p=0,0001; 160.2 mg/dL vs. 197.6 mg/dL, p=0.0001; 144.8 mg/dL vs. 167.0 mg/dL,p=0.0001; respectively). Moreover, in group B the average week of labor was earlier than in group A (mean 38,1 and 38,5 weeks of gestation, p=0,0006).

Conclusions: Patients who fulfilled more than one diagnostic criterion for GDM may have worse pregnancy outcome. We think that a number of fulfilled diagnostic criteria for GDM may be an important risk factor for insulin therapy during pregnancy and earlier gestational age at delivery.
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http://dx.doi.org/10.5603/GP.a2018.0065DOI Listing
October 2018

Systematic and taxonomic observations on the subfamily Synapseudinae Guţu, 1972 and related metapseudid taxa (Crustacea: Tanaidacea: Apseudomorpha), with the erection of a new genus and descriptions of three new species.

Zootaxa 2018 Jan 12;4370(4):301-344. Epub 2018 Jan 12.

Department of Coastal Sciences, Gulf Coast Research Campus, University of Southern Mississippi, Ocean Springs, Mississippi 39564, USA..

The tanaidacean metapseudid subfamily Synapseudinae Guţu is reviewed, partially revised and the type species, Synapseudes minutus Miller, redescribed. As rediagnosed and defined here, the Synapseudinae is restricted to the genera Synapseudes Miller, Vicinisyndes Guţu, and Creefs Stępień Błażewicz-Paszkowycz. The genera Curtipleon Băcescu and Cryptapseudes Băcescu are excluded from this subfamily. Three new species, S. caleyi, S. singularis, and S. hansknechti are described, bringing the number of putative species within the genus to 26. An identification key and table of selected specific characters for the species of Synapseudes are provided. A new monotypic genus, Ronabus, which is placed within the subfamily Metapseudinae Lang, is erected to receive Synapseudes idios Gardiner. The present taxonomic status of subfamilies within the Metapseudidae is briefly assessed.
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http://dx.doi.org/10.11646/zootaxa.4370.4.1DOI Listing
January 2018

Bryozoan zooid size variation across a bathymetric gradient: a case study from the Icelandic shelf and continental slope.

Mar Biol 2017 7;164(10):197. Epub 2017 Sep 7.

The Icelandic Institute of Natural History, Urriðaholtsstræti 6-8, 210 Garðabær, Iceland.

Body size is one of the most important biological characters, as it defines many aspects of organismal functioning at the individual and community level. As body size controls many ecological aspects of species, it is often used as a proxy for the status of the ecosystem. So far no consistent mechanism driving size shift has been proposed. In this study, we investigated bathymetric variability in zooid's size and shape in aquatic colonial animals, Bryozoa. Although the response of bryozoan zooid size to temperature or food concentration has been experimentally proven, the effects of natural environmental variability on marine bryozoan populations has been much less explored. The presented investigation is aimed to assess the bathymetric patterns and environmental drivers of bryozoan zooid size on continental shelf and slope of southern Iceland. 196 colonies of 11 species representing different colonial forms and taxonomic groups were selected for zooid characteristics measurements. A pattern of depth-related increase in zooid size was documented for , and , no statistically significant effects were detected for the other eight species. Two species and had significantly longer zooids in deeper water, shape of the remaining species did not change along the bathymetric gradient. Intercolonial coefficient of variation in zooid size did not change across the depth gradient. Temperature differences along studied depth could be responsible for the observed pattern.
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http://dx.doi.org/10.1007/s00227-017-3231-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5589789PMC
September 2017

A Neural Code That Is Isometric to Vocal Output and Correlates with Its Sensory Consequences.

PLoS Biol 2016 Oct 10;14(10):e2000317. Epub 2016 Oct 10.

Institute of Neuroinformatics, Neuroscience Center Zurich, University of Zurich/ETH Zurich, Zurich, Switzerland.

What cortical inputs are provided to motor control areas while they drive complex learned behaviors? We study this question in the nucleus interface of the nidopallium (NIf), which is required for normal birdsong production and provides the main source of auditory input to HVC, the driver of adult song. In juvenile and adult zebra finches, we find that spikes in NIf projection neurons precede vocalizations by several tens of milliseconds and are insensitive to distortions of auditory feedback. We identify a local isometry between NIf output and vocalizations: quasi-identical notes produced in different syllables are preceded by highly similar NIf spike patterns. NIf multiunit firing during song precedes responses in auditory cortical neurons by about 50 ms, revealing delayed congruence between NIf spiking and a neural representation of auditory feedback. Our findings suggest that NIf codes for imminent acoustic events within vocal performance.
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http://dx.doi.org/10.1371/journal.pbio.2000317DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5056755PMC
October 2016

Identification of miRSNPs associated with the risk of multiple myeloma.

Int J Cancer 2017 Feb 9;140(3):526-534. Epub 2016 Nov 9.

Genomic Epidemiology Group, German Cancer Research Center (DKFZ), Heidelberg, Germany.

Multiple myeloma (MM) is a malignancy of plasma cells usually infiltrating the bone marrow, associated with the production of a monoclonal immunoglobulin (M protein) which can be detected in the blood and/or urine. Multiple lines of evidence suggest that genetic factors are involved in MM pathogenesis, and several studies have identified single nucleotide polymorphisms (SNPs) associated with the susceptibility to the disease. SNPs within miRNA-binding sites in target genes (miRSNPs) may alter the strength of miRNA-mRNA interactions, thus deregulating protein expression. MiRSNPs are known to be associated with risk of various types of cancer, but they have never been investigated in MM. We performed an in silico genome-wide search for miRSNPs predicted to alter binding of miRNAs to their target sequences. We selected 12 miRSNPs and tested their association with MM risk. Our study population consisted of 1,832 controls and 2,894 MM cases recruited from seven European countries and Israel in the context of the IMMEnSE (International Multiple Myeloma rESEarch) consortium. In this population two SNPs showed an association with p < 0.05: rs286595 (located in gene MRLP22) and rs14191881 (located in gene TCF19). Results from IMMEnSE were meta-analyzed with data from a previously published genome-wide association study (GWAS). The SNPs rs13409 (located in the 3'UTR of the POU5F1 gene), rs1419881 (TCF19), rs1049633, rs1049623 (both in DDR1) showed significant associations with MM risk. In conclusion, we sought to identify genetic polymorphisms associated with MM risk starting from genome-wide prediction of miRSNPs. For some mirSNPs, we have shown promising associations with MM risk.
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http://dx.doi.org/10.1002/ijc.30465DOI Listing
February 2017

Functional single nucleotide polymorphisms within the cyclin-dependent kinase inhibitor 2A/2B region affect pancreatic cancer risk.

Oncotarget 2016 08;7(35):57011-57020

Genomic Epidemiology Group, German Cancer Research Center (DKFZ), Heidelberg, Germany.

The CDKN2A (p16) gene plays a key role in pancreatic cancer etiology. It is one of the most commonly somatically mutated genes in pancreatic cancer, rare germline mutations have been found to be associated with increased risk of developing familiar pancreatic cancer and CDKN2A promoter hyper-methylation has been suggested to play a critical role both in pancreatic cancer onset and prognosis. In addition several unrelated SNPs in the 9p21.3 region, that includes the CDNK2A, CDNK2B and the CDNK2B-AS1 genes, are associated with the development of cancer in various organs. However, association between the common genetic variability in this region and pancreatic cancer risk is not clearly understood. We sought to fill this gap in a case-control study genotyping 13 single nucleotide polymorphisms (SNPs) in 2,857 pancreatic ductal adenocarcinoma (PDAC) patients and 6,111 controls in the context of the Pancreatic Disease Research (PANDoRA) consortium. We found that the A allele of the rs3217992 SNP was associated with an increased pancreatic cancer risk (ORhet=1.14, 95% CI 1.01-1.27, p=0.026, ORhom=1.30, 95% CI 1.12-1.51, p=0.00049). This pleiotropic variant is reported to be a mir-SNP that, by changing the binding site of one or more miRNAs, could influence the normal cell cycle progression and in turn increase PDAC risk. In conclusion, we observed a novel association in a pleiotropic region that has been found to be of key relevance in the susceptibility to various types of cancer and diabetes suggesting that the CDKN2A/B locus could represent a genetic link between diabetes and pancreatic cancer risk.
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http://dx.doi.org/10.18632/oncotarget.10935DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5302969PMC
August 2016

[Evaluation of repetitive transcranial magnetic stimulation effectiveness in treatment of psychiatric and neurologic diseases].

Pol Merkur Lekarski 2016 Jun;40(240):388-92

Department of Neurology, Central Clinical Hospital of the Ministry of National Defense, Military Institute of Medicine in Warsaw, Poland.

Repetitive transcranial magnetic stimulation (rTMS) is a treatment option with proved effectiveness especially in drug resist depression. It is used in functional brain mapping before neurosurgery operations and diagnostic of corticospinal tract transmission. Many studies are performed to evaluate rTMS using in treatment of obsessive - compulsive disorder, schizophrenia, autism, strokes, tinnitus, Alzheimer and Parkinson diseases, cranial traumas. Moreover rTMS was used in treatment of multiple sclerosis, migraine, dystonia. Electromagnetical field generated by rTMS penetrate skin of the scalp and infiltrate brain tissues to a depth of 2 cm, cause neurons depolarization and generating motor, cognitive and affective effects. Depending on the stimulation frequency rTMS can stimuli or inhibit brain cortex. rTMS mechanism of action remains elusive. Probably it is connected with enhancement of neurotransmitters, modulation of signals transductions pathways in Central Nervous System, gene transcription and release of neuroprotective substances. Studies with use of animals revealed that rTMS stimulation can generate brain changes similar to those seen after electric shock therapy without provoking seizures. The aim of presenting study was to analyze actual researches evaluating rTMS use in treatment of psychiatric and neurological diseases.
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June 2016

First record of Tanaidacea (Crustacea) from a deep-sea coral reef in the Gulf of Guinea.

Zootaxa 2015 Aug 5;3995:203-28. Epub 2015 Aug 5.

Department of Polar Biology and Oceanobiology, University of Łódź, Banacha 12/16, 90-237 Łódź, Poland; Email: unknown.

Four undescribed species of Tanaidacea were discovered during a baseline monitoring program conducted off the coast of Ghana. The specimens came from a deep-water reef largely composed of the ahermatypic coral, Lophelia pertusa. The tanaidacean material was collected during November 2012 onboard the RV Dr Fridtjof Nansen using a van Veen grab in depths of between 375 and 386 m. Three of the new species described herein are tanaidomorphans belonging to the genera Bathyleptochelia (Leptocheliidae), Pseudotanais (Pseudotanaidae) and Cryptocopoides (Cryptocopidae). The fourth species, an apseudomorphan, belongs to Calozodion (Metapseudidae), a genus hitherto known only from shallow waters (<200 m). This report constitutes the first records of tanaidaceans from a deep-sea Lophelia reef.
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http://dx.doi.org/10.11646/zootaxa.3995.1.18DOI Listing
August 2015

A re-description of Discoconchoecia elegans (Sars, 1865) (Ostracoda: Halocyprididae) from high latitudes in the North Atlantic.

Zootaxa 2015 Aug 5;3995:66-77. Epub 2015 Aug 5.

UK National Oceanographic Centre, Southampton University, Southampton SO14 3ZH, UK; Email: unknown.

Discoconchoecia elegans (Sars, 1865) is one of the most frequently recorded species of halocyprid ostracods and specimens are collected in abundance from various latitudes throughout the world oceans. This species is often dominant or subdominant member of the mesopelagic assemblages. However, its body size varies substantially with latitude, posing the question as to whether D. elegans is either a single, highly variable species, or a complex of cryptic and sibling species. Evaluation of the hypothesis that D. elegans is a complex of species requires comparison between the type material and specimens collected from different latitudes. The inadequacy of the original description from the type locality, off the Lofoten Islands (NW Norway), combined with a lack of the type material is preventing critical rating. In this paper Discoconchoecia elegans is redescribed from specimens collected from an area close to Svalbard, using detailed drawings, morphometric measurements of all limbs, and SEM photographs, and it is compared with specimens collected from an area close to the species type locality. The individuals from those two localities show no significant differences, probably because the hydrographic conditions are similar between the two sites.
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http://dx.doi.org/10.11646/zootaxa.3995.1.9DOI Listing
August 2015

The role of WWOX tumor suppressor gene in the regulation of EMT process via regulation of CDH1-ZEB1-VIM expression in endometrial cancer.

Int J Oncol 2015 16;46(6):2639-48. Epub 2015 Apr 16.

Department of Molecular Carcinogenesis, Medical University of Lodz, PL 90-752 Lodz, Poland.

This study defines the role of WWOX in the regulation of epithelial to mesenchymal transition. A group of 164 endometrial adenocarcinoma patients was studied as well as an ECC1 well-differentiated steroid-responsive endometrial cell line, which was transducted with WWOX cDNA by a retroviral system. The relationship between WWOX gene and EMT marker (CDH1, VIM, ZEB1, SNAI1) expression on mRNA (RT-qPCR) and protein levels (western blotting) was evaluated. The EMT processes were also analysed in vitro by adhesion of cells to extracellular matrix proteins, migration through a basement membrane, anchorage-independent growth and MMP activity assay. DNA microarrays (HumanOneArray™) were used to determine WWOX-dependent pathways in an ECC1 cell line. A positive correlation was observed between WWOX and ZEB1, and a negative correlation between CDH1 and VIM. WWOX expression was found to inversely correlate with the risk of recurrence of tumors in patients. However, in the WWOX-expressing ECC1 cell line, WWOX expression was found to be inversely related with VIM and positively with CDH1. The ECC1/WWOX cell line variant demonstrated increased migratory capacity, with increased expression of metalloproteinases MMP2/MMP9. However, these cells were not able to form colonies in suspension and revealed decreased adhesion to fibronectin and fibrinogen. Microarray analysis demonstrated that WWOX has an impact on the variety of cellular pathways including the cadherin and integrin signalling pathways. Our results suggest that the WWOX gene plays a role in the regulation of EMT processes in endometrial cancer by controlling the expression of proteins associated with cell motility, thus influencing tissue remodeling, with the suppression of mesenchymal markers.
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http://dx.doi.org/10.3892/ijo.2015.2964DOI Listing
February 2016

Assessment of the relationship between lipid parameters and obesity indices in non-diabetic obese patients: a preliminary report.

Med Sci Monit 2014 Dec 16;20:2683-8. Epub 2014 Dec 16.

Department of Nephrology, Hypertension and Family Medicine, Medical University of Łódź, Łódź, Poland.

Background: The aim of this cross-sectional study was to examine the relationship between obesity and lipid markers.

Material And Methods: We divided 66 non-diabetic adult obese patients (mean age: 55.8±11.6 years) into 3 groups according to body mass index (BMI). All patients were measured for waist circumference (WC), hip circumference (HC), body mass index (BMI), waist-to-hip ratio (WHR), waist-to-height ratio (WHtR), body adiposity index (BAI), and visceral adiposity index (VAI). Serum levels of total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), and triglycerides (TG) were determined, and lipid indices TC/HDL, LDL/HDL, and TG/HDL were also estimated.

Results: TC and LDL-C in Group III were lower than in Group I (5.0±1.0 vs. 6.0±1.0 mmol/L, and 2.9±0.9 vs. 3.8±1.2 mmol/L; p<0.05 for both). Negative correlations were found between: BMI and TC, LDL, and HDL (r=-0.291; r=-0.310, r=-0.240, respectively); and WC, WHR, VAI, and HDL (r=-0.371, r=-0.296, r=-0.376, respectively). Positive correlations were found between WC, WHR, and TG/HDL (r=0.279, r=0.244, respectively) and between VAI and: TC (r=0.327), TG (r=0.885), TC/HDL (r=0.618), LDL/HDL (r=0.480), and TG/HDL (r=0.927).

Conclusions: Obesity is associated with lipid disturbances, especially with HDL-C reduction, in obese non-diabetic patients. VAI is strongly related to lipid profile and thus may be the most valuable obesity index in obese patients with dyslipidemias.
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http://dx.doi.org/10.12659/MSM.890845DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4271804PMC
December 2014

Alternating expression levels of tumor suppressor and cancer-related genes in patients with bladder cancer.

Oncol Lett 2014 Nov 22;8(5):2291-2297. Epub 2014 Aug 22.

Department of Molecular Cancerogenesis, Medical University of Lodz, Lodz 90-752, Poland.

The aim of the present study was to determine the roles of the tumor suppressor and cancer-related genes in bladder tumor carcinogenesis. Reverse transcription-quantitative polymerase chain reaction was used to analyze the status of promoter methylation (using MethylScreen™ technology) and loss of heterozygosity (LOH) in papillary urothelial cancer tissues. The associations between the expression levels of the following tumorigenesis-related genes were also assessed: The tumor suppressor gene the proliferation gene, the and apoptotic genes, the signal transduction gene, the vascular endothelial growth factor gene, and the and cell cycle genes. The results reveal a high frequency of LOH in intron 1 in the gene, as well as an association between reduced expression levels and increased promoter methylation. In addition, the present study demonstrates that in bladder tumors, apoptosis is inhibited by increased expression levels of the gene. A correlation between the proliferation indices of the and the genes was also revealed Furthermore, the expression levels of were identified to be positively associated with those of the gene.
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http://dx.doi.org/10.3892/ol.2014.2476DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4186597PMC
November 2014

Risk of multiple myeloma is associated with polymorphisms within telomerase genes and telomere length.

Int J Cancer 2015 Mar 6;136(5):E351-8. Epub 2014 Aug 6.

Division of Cancer Epidemiology, German Cancer Research Center (DKFZ), Heidelberg, Germany.

Compelling biological and epidemiological evidences point to a key role of genetic variants of the TERT and TERC genes in cancer development. We analyzed the genetic variability of these two gene regions using samples of 2,267 multiple myeloma (MM) cases and 2,796 healthy controls. We found that a TERT variant, rs2242652, is associated with reduced MM susceptibility (OR = 0.81; 95% CI: 0.72-0.92; p = 0.001). In addition we measured the leukocyte telomere length (LTL) in a subgroup of 140 cases who were chemotherapy-free at the time of blood donation and 468 controls, and found that MM patients had longer telomeres compared to controls (OR = 1.19; 95% CI: 0.63-2.24; p(trend)  = 0.01 comparing the quartile with the longest LTL versus the shortest LTL). Our data suggest the hypothesis of decreased disease risk by genetic variants that reduce the efficiency of the telomerase complex. This reduced efficiency leads to shorter telomere ends, which in turn may also be a marker of decreased MM risk.
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http://dx.doi.org/10.1002/ijc.29101DOI Listing
March 2015

Obesity indices and inflammatory markers in obese non-diabetic normo- and hypertensive patients: a comparative pilot study.

Lipids Health Dis 2014 Feb 8;13:29. Epub 2014 Feb 8.

Department of Nephrology, Hypertension and Family Medicine, Medical University of Lodz, Żeromskiego str, 113, 90-549 Lodz, Poland.

Background: The aim of this study was to estimate associations between inflammatory markers and obesity indices in normo- and hypertensive subjects.

Methods: 65 obese adult subjects were divided into two groups: (A) of hypertensives (n = 54) and (B) of normotensives (n = 11). Waist circumference (WC), body mass index (BMI), waist-to-hip ratio (WHR), waist-to-height ratio (WHtR), visceral adiposity index (VAI), body adiposity index (BAI) and tumor necrosis factor-α (TNF-α), interleukin (IL)-6 and high sensitivity C-reactive protein (hsCRP) serum concentrations were estimated.

Results: In group A WHtR was higher (0.69 ± 0.07 vs 0.63 ± 0.06; p < 0.01), hsCRP correlated with BMI and WHtR (r = 0.343; p = 0.011 and r = 0.363; p < 0.01, respectively). BAI correlated with hsCRP in group A and B (r = 0.329; p < 0.05 and r = 0.642; p < 0.05; respectively) and in females and males (r = 0.305; p = 0.05 and r = 0.44; p < 0.05, respectively). In females hsCRP was higher (3.2 ± 2.2 mg/l vs 2.1 ± 1.5 mg/l; p < 0.05). In patients without lipid lowering treatment hsCRP and IL-6 were higher (3.2 ± 1.7 mg/l vs 2.4 ±2.2 mg/l; p = 0.01 and 15.9 ± 7.2 pg/ml vs 13.6 ± 9.9 pg/ml; p < 0.01, respectively).

Conclusions: WHtR is a sensitive index associated with chronic inflammation in obese hypertensive subjects. BAI correlates with hsCRP independently of hypertension and sex. hsCRP is more sensitive marker associated with obesity than IL-6 and TNF-α. Lipid lowering treatment influence chronic inflammation.
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http://dx.doi.org/10.1186/1476-511X-13-29DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3921991PMC
February 2014

Obesity indices and adipokines in non-diabetic obese patients with early stages of chronic kidney disease.

Med Sci Monit 2013 Nov 27;19:1063-72. Epub 2013 Nov 27.

Background: The aim of this study was to estimate obesity parameters: waist circumference (WC), waist-to-hip ratio (WHR), weight-to-height ratio (WHtR), visceral adiposity index (VAI), body adiposity index (BAI), and serum adipokines (leptin, adiponectin, resistin) and their associations with estimated glomerular filtration rate (eGFR), serum creatinine, and microalbuminuria (MA) in patients with early stages of CKD and in non-CKD obese patients.

Material And Methods: 67 non-diabetic obese (BMI ≥ 30 mg/kg(2) out-clinic patients (25 males, 42 females), aged from 36.5 to 64 years were divided into 2 groups: Group A (n=15)--patients with early stages of CKD (eGFR between 30 and 60 ml/min/1.73 m(2) or with MA >20 mg/l in morning urine sample independently from GFR) and Group B--patients without chronic CKD (n=52).

Results: In Group A compared to Group B, BAI and leptin were higher (42.2 ± 7.1 vs. 37.5 ± 7.0; p<0.05 and 51.8 ± 26.7 ng/mL vs. 35.3 ± 24.9 ng/mL; p<0.05; respectively) and negative correlations occurred between eGFR and BAI (r=-0.709; p=0.003), leptin (r=-0.68; p=0.005), and resistin (r=-0.528; p<0.05). In Group B, negative correlations occurred between creatinine and VAI (r=-0.332; p<0.05), BAI (r=-0.619; p<0.0001), leptin (r=-0.676; p<0.0001), and adiponectin (r=-0.423; p=0.002), and between eGFR and resistin (r=-0.276; p<0.05).

Conclusions: BAI may be a valuable obesity parameter as a predictor of early stages of CKD in patients with obesity. Leptin may be an important pathogenic factor in obese patients with early stages of CKD. Resistin is associated with eGFR in obese patients, independently of CKD.
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http://dx.doi.org/10.12659/MSM.889390DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3852621PMC
November 2013

A new species of Numbakullidae Guţu & Heard, 2002 (Tanaidacea, Peracarida, Crustacea) from the Great Barrier Reef, Australia.

Authors:
Anna Stępień

Zookeys 2013 1(337):35-47. Epub 2013 Oct 1.

Department of Invertebrate Zoology and Hydrobiology, University of Łódź, Banacha 12/16, Łódź 90-237, Poland.

A new species of Numbakulla Guţu & Heard, 2002 (Tanaidacea) is described from Heron Island (southern Great Barrier Reef, Queensland) collected during the Census of Coral Reefs Ecosystem (CReefs) program. The new species is the third member of the family and can be recognized by the combination of characters as: length/width ratio of the body, which is 6:7, pereonite 4 longer than the rest, the presence of eyes, a blunt rostrum, antenna article 2 elongated, cheliped carpus with row of inner setae, pereopod 6 carpus with spines, pleopod endopod with denticles.
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http://dx.doi.org/10.3897/zookeys.337.5903DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3800784PMC
October 2013

New obesity indices and adipokines in normotensive patients and patients with hypertension: comparative pilot analysis.

Angiology 2014 Apr 1;65(4):333-42. Epub 2013 May 1.

1Department of Nephrology, Hypertension and Family Medicine, WAM University Hospital in Lodz, Medical University of Lodz, Lodz, Poland.

We compared the obesity parameters and selected adipokines-leptin, adiponectin, and resistin-in obese patients with hypertension and normotensive patients. A total of 67 nondiabetic obese outpatients were divided into 2 groups: A-hypertensive and B-normotensive. Serum levels of leptin, adiponectin, resistin, and insulin were measured. Weight, height, waist circumference, and hip circumference were measured to calculate waist-to-hip ratio (WHR), weight-to-height ratio, visceral adiposity index, and body adiposity index (BAI). Among patients with hypertension, significant positive correlations were observed between leptin and body mass index and BAI (r = .31 and r = .63, respectively). In normotensive patients, leptin positively correlated with BAI (r = .73, P < .01) and negatively with WHR (r = -.55, P < .0001); adiponectin negatively correlated with WHR (r = .38, P < .01) and BAI (r = .52; P < .0001), and resistin negatively correlated with WHR (r = -.36, P < .05). In conclusion, visceral obesity and leptin are associated with hypertension in obese patients.
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http://dx.doi.org/10.1177/0003319713485807DOI Listing
April 2014

The use of high frequency ultrasound imaging in skin moisturization measurement.

Skin Res Technol 2013 May 9;19(2):169-75. Epub 2013 Jan 9.

Department of Diagnostic Imaging of the II Medical Faculty of the Medical, University of Warsaw, Warszawa, Poland.

Introduction: The appropriate skin hydration level enables its normal function and healthy appearance.

Purpose: The purpose of present research was to assess the applicability of high frequency ultrasound (HFU) to the monitoring of skin moisturization treatments.

Material And Methods: The study sample encompassed 27 women, aged 20-67 y.o. (mean age of 45.48 y.o.) with dry skin. All women applied a strong moisturizing cream on their facial skin for 14 days. The course of treatment was monitored using the HFU. The following parameters were subjected to the ultrasound evaluation: epidermal echo thickness, dermis thickness, and separately the thickness of the superior and inferior layer of dermis. The measurements were taken on the participants' chins and cheeks. In addition, skin hydration and transepidermal water loss (TEWL) were determined.

Results: Statistically significant differences were obtained between the echogenicity of the superior layer of the dermis on the chin and cheek. After treatment, the statistically significant values of TEWL decrease and hydration increase were obtained on the cheek skin. No statistically significant differences between the TEWL or hydration levels were found on the chin. No statistically significant differences between the epidermal echo and dermis thickness values for the two measurement points were observed.

Conclusion: The HFU is a useful method for the monitoring of skin moisturization treatments.
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http://dx.doi.org/10.1111/srt.12029DOI Listing
May 2013

Four new species and two new genera of Metapseudidae (Crustacea: Tanaidacea: Apseudomorpha) from Australian coral reefs.

Zootaxa 2013 ;3717:559-92

Department of Invertebrate Zoology and Hydrobiology, University of Łódź, Banacha 12/16, Łódź 90-237, Poland.

This paper presents the descriptions of two new genera and four new metapseudid species collected from the coral reefs of Ningaloo and Heron Island in north-western and eastern Australia, respectively. Two of the species, Curtipleon chadi n. sp., and Creefs heronum n. gen. n. sp. are members of the subfamily Synapseudinae, one, Msangia mussida n. sp. is a member of the subfamily Msangiinae and one Bamberus jinigudirus n. gen. n. sp. is a member of the subfamily Chondropodinae. With the exception of M mussida that was found on live coral, all of the species were associated with dead corals.
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http://dx.doi.org/10.11646/zootaxa.3717.4.7DOI Listing
August 2015