Publications by authors named "Anna Babinska"

57 Publications

Short-Term Resistance Training Supported by Whole-Body Cryostimulation Induced a Decrease in Myostatin Concentration and an Increase in Isokinetic Muscle Strength.

Int J Environ Res Public Health 2020 07 30;17(15). Epub 2020 Jul 30.

Department of Athletics, Strength and Conditioning, Poznan University of Physical Education, Krolowej Jadwigi 27/39, 61-871 Poznan, Poland.

The study aimed to determine whether combining cryostimulation with resistance training would effectively increase muscle strength, and if so, whether this adaptation would be related to changes in circulating levels of exerkines (i.e., mediators of systemic adaptation to exercise). Twenty-five students completed 12 sessions of resistance training, each followed by either cryostimulation ( = 15, 3 min exposure at -110 °C) or passive recovery ( = 10). Prior to and post this intervention, participants performed two eccentric cycling bouts (before and after training). At these points, serum concentrations of muscle damage marker (myoglobin), exerkines (interleukin 6 (IL-6), interleukin 15 (IL-15), irisin, brain-derived neurotrophic factor), hypertrophy-related factors (myostatin, insulin-like growth factor 1), and muscle strength were measured. The applied procedure reduced the physiological burden of the second eccentric cycling bout and myoglobin concentrations only in the group subject to cryostimulation. The same group also exhibited decreased levels of myostatin (from 4.7 ± 1.7 to 3.8 ± 1.8 ng·mL, < 0.05). A significant and large interaction between the group × time was noted in IL-15 concentration ( = 0.01, ηp2=0.27). Training and cryostimulation induced a positive and likely significant improvement of isokinetic muscle strength. Altogether, obtained results support the claim that resistance training combined with cold exposure modified muscle strength through modulation of myostatin and IL-15 concentrations.
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http://dx.doi.org/10.3390/ijerph17155496DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7432449PMC
July 2020

data: treatment with the F11R/JAM-A peptide 4D decreases mortality and reduces the generation of atherosclerotic plaques in ApoE-deficient mice.

Data Brief 2020 Jun 23;30:105516. Epub 2020 Apr 23.

Department of Medicine, State University of New York, Downstate Medical Center, Brooklyn, New York 11203, USA.

The data in this article focus on the F11 Receptor (F11R/JAM-A; Junctional Adhesion Molecule-A; JAM-A, F11R), a cell adhesion protein constitutively expressed on the membrane surface of circulating platelets and localized within the tight junctions of healthy endothelial cells (ECs). Previous reports have shown that F11R/JAM-A plays a critical role in the adhesion of platelets to an inflamed endothelium due to its' pathological expression on the luminal surface of the cytokine-inflamed endothelium. Since platelet adhesion to an inflamed endothelium is an early step in the development of atherosclerotic plaque formation, and with time, resulting in heart attacks and stroke, we conducted a long-term, study utilizing the atherosclerosis-prone ApoE mice to attempt a blockade of the formation of atherosclerotic plaques by preventing the adhesion of platelets to the inflamed vasculature . Utilizing a nonhydrolyzable peptide derived from an amino acid sequence of F11R/JAM-A, peptide 4D, we have shown that the adhesion of platelets to the inflamed endothelial cells could be blocked by peptide 4D. The present data demonstrate the positive health benefits of chronic peptide 4D administration to the atherosclerosis-prone ApoE mice, and provides new information for potential use of this F11R derived peptide in the prevention of atherosclerosis. The data presented in this article provide further experimental support for the study presented in Babinska et al., Atherosclerosis 284 (2019) 92-101.
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http://dx.doi.org/10.1016/j.dib.2020.105516DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7206208PMC
June 2020

Selected adipocytokines in patients with an incidentally discovered pheochromocytoma.

Minerva Endocrinol 2020 Jun 13;45(2):117-126. Epub 2019 Nov 13.

Department of Endocrinology and Internal Medicine, Medical University of Gdansk, Gdansk, Poland.

Background: Adipose tissue secretes many adipokines and cytokines, which may be an additional risk factor of cardiovascular and metabolic diseases in patients with an incidentally discovered pheochromocytoma (PHEO). The aim of the study was to investigate levels of selected adipocytokines in these patients.

Methods: This prospective study included 12 patients with an incidentally discovered PHEO and 18 healthy participants. In all participants plasma/serum concentrations of triglycerides, HDL and LDL cholesterol, insulin, glucose, adipocytokines (adiponectin, leptin, resistin, TNFα, IL6, and MCP1) were determined, hormonal tests were performed in patients.

Results: Patients and controls did not differ significantly in terms of age, sex, and body mass index. Among incidentally discovered PHEO patients, adiponectin levels were lower, while TNFα concentrations higher than in controls. Concentrations of adiponectin correlated with 24-hour urinary excretion of normetanephrine in women. Significantly higher TNFα concentrations were found in hypertensive than in normotensive PHEO patients as well as in non-diabetic PHEO patients than controls. Further, resistin concentration was higher in PHEO patients with diabetes than in non-diabetic ones (P<0.001). Incidentally discovered PHEO tumor size correlated with leptin and IL6 levels. Adiponectin levels were higher, while TNFα and resistin lower among five patients re-examined after tumor resection.

Conclusions: Among patients with an incidentally discovered pheochromocytoma, lower adiponectin, and higher resistin and TNFα levels may constitute additional factors for HT and DM. In our study, for the first time, correlations between incidentally discovered PHEO tumor size and concentrations of leptin as well as IL6 were found.
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http://dx.doi.org/10.23736/S0391-1977.19.03007-4DOI Listing
June 2020

Functional inhibition of F11 receptor (F11R/junctional adhesion molecule-A/JAM-A) activity by a F11R-derived peptide in breast cancer and its microenvironment.

Breast Cancer Res Treat 2020 Jan 24;179(2):325-335. Epub 2019 Oct 24.

Department of Cytobiology and Proteomics, Medical University of Lodz, Lodz, Poland.

Purpose: To examine the involvement of the F11R/JAM-A protein in breast cancer metastasis, we utilized the F11R/JAM-A antagonistic peptide 4D (P4D) in experiments of transendothelial migration (TEM) of breast cancer cells.

Methods: Experiments were conducted in the mouse 4T1 breast cancer model utilizing the human mammary epithelial cell and endothelial cell lines. The levels of soluble F11R/JAM-A (sJAM-A) in the murine plasmas were measured by ELISA. Levels of F11R/JAM-A mRNA and protein in cell lines were assessed by qRT-PCR and Western blot, respectively. Cell surface expression of F11R/JAM-A was demonstrated by flow cytometry. Functional tests included the TEM of breast cancer cells and adhesion of breast cancer cells to the endothelium. The endothelial permeability was studied by fluorescent tracer assay and by the Real-Time Cell Analysis (RTCA).

Results: The tumor inducers Tβ4 and TGF-β1 reduced the levels of sJAM-A in murine plasma, and reduced the F11R/JAM-A protein levels in the human microvascular endothelial cell line HMEC-1. The adhesion and TEM measured between breast cancer cells and inflamed or Tβ4-treated endothelium were inhibited by P4D. The presence of P4D did not destabilize the pre-existing tight junctions in the endothelial monolayer. The barrier-protecting effect of P4D was stronger than that of forskolin, when a booster dose of P4D was applied to the inflamed endothelium.

Conclusions: F11R/JAM-A protein can be considered as a novel target in the treatment of breast cancer metastasis. In vivo and clinical studies are needed to further investigate the effectiveness of F11R/JAM-A-derived peptide as a possible anti-metastatic drug.
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http://dx.doi.org/10.1007/s10549-019-05471-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6987052PMC
January 2020

Expression of adiponectin receptors 1 and 2 and the leptin receptor in human adrenal tumors.

Arch Med Sci 2019 Sep 1;15(5):1254-1260. Epub 2018 Jun 1.

Department of Endocrinology and Internal Medicine, Medical University of Gdansk, Gdansk, Poland.

Introduction: The role of adipokines in neoplasms not related to obesity is unclear. The presence of adiponectin receptors 1 and 2 (AdipoR1 and AdipoR2) as well as the leptin receptor (Ob-R) has been recognized in human adrenal tumors. The authors of the present study were the first to compare the expression of these receptors in histopathologically distinct adrenal tumors.

Material And Methods: The study encompassed tissue specimens of 128 patients with adrenal tumors (28 adrenal cortical adenomas (CA), 35 cortical nodular hyperplasia tumors (CNH), 20 cortical carcinomas (CC), 40 pheochromocytomas (PHEO), 5 malignant pheochromocytomas (PHEOM)) operated on at a single clinical center. The expression of the adiponectin receptors AdipoR1 and AdipoR2 as well as the leptin receptor Ob-R was assessed by immunohistochemistry. The results were correlated with body mass index (BMI) and gender of the patients.

Results: AdipoR1 expression was significantly higher in cortical cancers ( < 0.001) and pheochromocytomas ( < 0.001) as compared to benign cortical tumors. AdipoR2 expression was significantly higher in cortical carcinomas as compared to cortical adenomas and hyperplasia tumors ( = 0.01), and also significantly higher in pheochromocytomas in comparison to adrenocortical cancers ( = 0.004). Leptin receptor expression was absent or minimal in half of nodular hyperplasia tumors and adrenal cortex adenomas. This receptor's expression was significantly higher in adrenocortical cancers ( = 0.038). In pheochromocytomas this receptor was expressed more abundantly than in adrenocortical cancers ( = 0.004).

Conclusions: These novel findings suggest that adiponectin and leptin receptors could play a regulatory role in human adrenal neoplasms.
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http://dx.doi.org/10.5114/aoms.2018.76142DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6764317PMC
September 2019

A peptide antagonist of F11R/JAM-A reduces plaque formation and prolongs survival in an animal model of atherosclerosis.

Atherosclerosis 2019 05 22;284:92-101. Epub 2019 Feb 22.

Department of Medicine, State University of New York, Downstate Medical Center, Brooklyn, NY, 11203, USA.

Background And Aims: The F11 Receptor (F11R), AKA Junctional Adhesion Molecule-A (JAM-A) (F11R/JAM-A), is an adhesion protein constitutively expressed on the membrane surface of circulating platelets and the luminal surface of inflamed endothelial cells (EC). Platelet adhesion to an inflamed endothelium is one of the early steps of atherosclerotic plaque formation. Our previous studies, conducted with cultured EC in vitro, have demonstrated the expression of F11R/JAM-A on the luminal surface of inflamed EC, platelet adhesion to inflamed EC through F11R/JAM-A interactions, and inhibition of this interaction by the presence of F11R/JAM-A antagonistic peptide (F11Rpeptide 4D). In the present study, we examined in vivo the overall health-benefits and cardiovascular effects of long-term treatment of animals prone to atherosclerosis, ApoE mice, with F11R-peptide 4D.

Methods: Twenty ApoE mice were assigned to daily treatment with peptide 4D and compared to their counterparts control untreated mice. Mice were observed for wellness and survival. Plaque size in the aorta and heart was measured using histological analysis. Effects of peptide 4D (or scramble control) on platelet adhesion to inflamed endothelium were measured using intravital microscopy.

Results: Significant reductions in atherosclerotic plaques number and size, an overall robust health with longer survival were found in the peptide 4D treated group of ApoE mice. Intravital microscopic studies conducted in exposed vessels of ApoE mice demonstrated significant inhibition by peptide 4D of platelet adhesion to the cytokine-inflamed endothelium.

Conclusions: Our results demonstrate that peptide 4D significantly reduces atherosclerotic plaque formation in ApoE mice and inhibits platelet adhesion to the inflamed arterial endothelium.
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http://dx.doi.org/10.1016/j.atherosclerosis.2019.02.014DOI Listing
May 2019

IgG4-related disease in endocrine practice.

Arch Med Sci 2019 Jan 20;15(1):55-64. Epub 2017 Oct 20.

Department of Endocrinology and Internal Medicine, Medical University of Gdansk, Gdansk, Poland.

IgG4-related disease is a set of symptoms resulting from a chronic, usually multiple organ inflammatory condition which affects various organs. It consists of lymphoplasmacytic infiltrations with attendant fibrosis and deep vein thrombosis. Frequently observed tissue lesions are accompanied by elevated IgG4 levels in serum. The etiopathogenesis of the lesions is of multifactor character and the clinical manifestation of the disease is highly diverse. The diagnostic process is based on the patient's medical history, clinical examination and additional tests, including a histopathological examination of the infected organ's tissues. Almost forty different locations of the disease have been reported, including disorders of the endocrine system. IgG4-related endocrinopathies are quite rare. However, it is likely that the diagnosis is under-reported due to lack of awareness of this clinical entity. Despite increasing interest in the subject, there are not enough reliable studies evaluating the link between IgG4-RD and endocrine disorders.
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http://dx.doi.org/10.5114/aoms.2017.70889DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6348348PMC
January 2019

No association between MRI changes in the lumbar spine and intensity of pain, quality of life, depressive and anxiety symptoms in patients with low back pain.

Neurol Neurochir Pol 2019;53(1):74-82. Epub 2019 Jan 7.

Department of Basic Medical Sciences, Faculty of Public Health, Medical University of Silesia, Katowice, Poland.

Introduction: The association between changes in magnetic resonance imaging (MRI) and clinical symptoms in patients with low back pain (LBP) is unclear.

Aim: To evaluate correlations between combined MRI findings of the lumbar spine (LS) and pain intensity, depressive and anxiety symptoms and quality of life in patients with LBP.

Material And Methods: 200 subjects (93 men and 107 women; mean age 51.42 ± 13.21 years) with LBP referred for MRI were enrolled in the study. All patients completed the Hospital Anxiety and Depression Scale (HADS), Quality of Life Scales (EQ-5D, EQ-VAS) and the Visual Analogue Scale (VAS). MRI scans were assessed according to a scoring system prepared by the authors, and the total MRI score was calculated.

Results: The mean total MRI score was 11.59 ± 6.73 points (range 0-50 points) and was higher in men than in women (p = 0.015). A correlation was observed between total MRI score and age (p < 0.001) and between total MRI score and BMI (p = 0.005). An association was found between total MRI score and EQ-5D (p = 0.012) and HADS-D results (p = 0.003). VAS and HADS-A results did not correlate with MRI score. When multivariate analysis was done, the total MRI score was only significantly related to age and BMI, and association between the total MRI score and EQ-5D or HADS-D results was not confirmed. Decreased quality of life was associated with increased intensity of pain and depressive and anxiety symptoms.

Conclusions: Combined MRI changes in LS do not correlate with pain intensity, depressive and anxiety syndromes or quality of life in patients with LBP.
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http://dx.doi.org/10.5603/PJNNS.a2018.0006DOI Listing
June 2019

A 2-Week Specific Volleyball Training Supported by the Whole Body Cryostimulation Protocol Induced an Increase of Growth Factors and Counteracted Deterioration of Physical Performance.

Front Physiol 2018 28;9:1711. Epub 2018 Nov 28.

Department of Physiology and Pharmacology, Faculty of Rehabilitation and Kinesiology, Gdansk University of Physical Education and Sport, Gdańsk, Poland.

Potentially beneficial effects of cold therapies on training adaptation still remain unequivocal. We have, thus, decided to evaluate the effects of a 2-week volleyball training program supported by 10 sessions of whole body cryostimulation (WBC) on growth factors and physical performance. Twenty healthy college-aged men and women randomly assigned either to the cryostimulation group (CRY) or the control group (CON; executed passive rest). Both groups took part in the same 2-weeks training program. Additionally, the CRY group attended in 10 cryo-sessions (3 min, -110°C temperature, five times/week). Blood samples were collected at baseline, 1 h after the first cryo-session as well as before and 1 h after the last session of WBC to assess growth factors, myokines concentration and the amino acid profile. Motor abilities were tested before commencing the training program and 2 days after its completion. The applied intervention resulted in an increase of brain-derived neurotrophic factor and insulin-like growth factor 1 concentrations. The adjusted effect describing the difference between groups in response to applied procedures was for both growth factors large and very likely in the CRY, higher than in the CON group (113%; Coefficient Interval: 38-230%, 45%; Coefficient Interval: 17-79%, respectively). Physical performance dropped in both groups, yet in the CRY group, the magnitude of change was smaller. The fibroblast growth factor dropped significantly 1 h following the first cryo-session, yet irisin remained statistically unchanged. The similar tendency was maintained after the whole procedure, still the range of changes was smaller. In the CRY group, an elevated uptake of tryptophan and valine noted in response to the whole intervention, could have induced a significant decrease of fasting glucose concentration (the adjusted effect small and very likely -6%; Coefficient Interval: -10 to -2%). Overall, a 2-week volleyball training program supported by the whole body cryostimulation protocol resulted in an increase of growth factors and offset a decline of physical performance. Thus these procedure can be applied in professional sport during competition period, especially among those disciplines focusing on an explosive power and ability to concentrate.
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http://dx.doi.org/10.3389/fphys.2018.01711DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6282029PMC
November 2018

Plasma Phospholipid Transfer Protein Promotes Platelet Aggregation.

Thromb Haemost 2018 Dec 12;118(12):2086-2097. Epub 2018 Nov 12.

Department of Cell Biology, SUNY Downstate Medical Center, Brooklyn, New York, United States.

It remains unclear whether plasma phospholipid transfer protein (PLTP) is involved in hyper-coagulation or hypo-coagulation. This study investigated the direct effect of PLTP on platelet aggregation and the underlying mechanism. Washed platelets from humans or mice and mouse platelet-rich plasma and human recombinant PLTP were isolated. PLTP is present in human platelets. We assessed adenosine diphosphate (ADP)-, collagen- and thrombin-induced platelet aggregation, phosphatidylserine externalization and photothrombosis-induced cerebral infarction in mice. PLTP over-expression increased platelet aggregation, while PLTP deficiency had the opposing reaction. Human recombinant PLTP increased both mouse and human platelet aggregation in a dose-dependent manner. Phosphatidylserine externalization provides a water/lipid surface for the interaction of coagulation factors, which accelerates thrombosis. Compared with wild-type controls, platelets from PLTP transgenic mice had significantly more phosphatidylserine on the exterior surface of the plasma membrane, whereas platelets from PLTP-deficient mice had significantly less phosphatidylserine on the surface, thus PLTP influences fibrinogen binding on the plasma membrane. Moreover, recombinant PLTP together with ADP significantly increased phosphatidylserine exposure on the plasma membrane of PLTP-deficient platelets, thereby increasing fibrinogen binding. PLTP over-expression significantly accelerated the incidence of photothrombosis-induced infarction in mice, whereas PLTP deficiency significantly reduced the frequency of infarction. We concluded that PLTP promotes phosphatidylserine externalization at the plasma membrane of platelets and accelerates ADP- or collagen-induced platelet aggregation. This effect plays an important role in the initiation of thrombin generation and platelet aggregation under sheer stress conditions. Thus, PLTP is involved in hyper-coagulation. Therefore, PLTP inhibition could be a novel approach for countering thrombosis.
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http://dx.doi.org/10.1055/s-0038-1675228DOI Listing
December 2018

Vitamin D Supplementation and Nordic Walking Training Decreases Serum Homocysteine and Ferritin in Elderly Women.

Int J Environ Res Public Health 2018 09 20;15(10). Epub 2018 Sep 20.

Faculty of Rehabilitation and Kinesiology, Department of Physiology and Pharmacology, Gdansk University of Physical Education and Sport, Gorskiego 1, 80-336 Gdansk, Poland.

The aim of the study was to verify if coupling 12 weeks of vitamin D supplementation and Nordic walking training favoured lowering the homocysteine (Hcy) level. Ninety-four elderly women were divided into three groups: Nordic walking (NW), supplemented (SG) and control (CG). The NW and SG groups received a weekly dose of 28,000 IU of vitamin D3. A blood analysis was performed at baseline, 1h after the first training session and at the end of the experiment. The amino acid profile (methionine and cysteine) and homocysteine concentration were determined. Additionally, the concentration of myokine was assessed. The first session of NW training reduced serum homocysteine, particularly among women with baseline homocysteine above 10 µmol·L: 12.37 ± 2.75 vs. 10.95 ± 3.94 µmol·L ( = 0.05). These changes were accompanied by shifts in the cysteine ( = 0.09) and methionine ( = 0.01) concentration, regardless of the Hcy concentration. Twelve weeks of training significantly decreased the homocysteine (9.91 ± 2.78, vs. 8.90 ± 3.14 µmol·L, = 0.05) and ferritin (94.23 ± 62.49 vs. 73.15 ± 47.04 ng·mL, = 0.05) concentrations in whole NW group. Also, in the NW group, ferritin correlated with the glucose level (r = 0.51, = 0.00). No changes in the myokine levels were observed after the intervention. Only the brain-derived neurotrophic factor dropped in the NW (42.74 ± 19.92 vs. 31.93 ± 15.91 ng·mL, = 0.01) and SG (37.75 ± 8.08 vs. 16.94 ± 12.78 ng·mL, = 0.00) groups. This study presents a parallel decrease of homocysteine and ferritin in response to regular training supported by vitamin D supplementation.
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http://dx.doi.org/10.3390/ijerph15102064DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6210625PMC
September 2018

Adipokine and cytokine levels in patients with adrenocortical cancer, subclinical Cushing's syndrome and healthy controls.

Steroids 2018 12 6;140:39-44. Epub 2018 Sep 6.

Department of Endocrinology and Internal Medicine, Medical University of Gdansk, ul. Dębinki 7, 80-288 Gdansk, Poland. Electronic address:

Introduction: In recent years researchers have focused at hormonal activity in Cushing's syndrome (CS) in connection with metabolic disorders and the role of adipokines and cytokines secreted by the adipose tissue. The aim of the study was to investigate levels of adipokines and cytokines in patients with: subclinical CS (SCS) - in relation to hormonal parameters of hypercortisolemia, and, adrenocortical cancer (ACC).

Materials And Methods: The study included 20 SCS as well as 7 ACC patients, and 18 healthy participants. Hormonal activity and serum concentrations of adiponectin, leptin, resistin, tumor necrosis factor alpha (TNFα), interleukin 6 (IL6), and monocyte chemoattractant protein 1 (MCP1), were analyzed.

Results: In SCS patients compared to healthy volunteers a trend toward higher concentrations of all pro-inflammatory cytokines was noted, however, statistically significant differences were only found for TNFα and IL6 (p = 0.047 and p = 0.028, respectively). Adiponectin concentrations were significantly lower in the SCS group (p = 0.006). Serum adipokine and cytokine levels were independent of the presence of diabetes mellitus (DM) and hypertension (HT) in the SCS group. A significant correlation was found between subclinical glucocorticoid secretion and IL6 concentration (Pearson's r = 0.517, p = 0.02). Acquired results were independent of BMI. In ACC patients compared to controls higher IL6, TNFα and MCP1 levels were recorded.

Conclusion: It is possible that higher adipokine and pro-inflammatory cytokine concentrations as well as lower anti-inflammatory adiponectin concentrations comprise an additional risk factor of metabolic and cardiovascular diseases in SCS patients. It seems that at least among patients with SCS adipokine and cytokine secretion is independent of hormonal activity (except for IL6).
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http://dx.doi.org/10.1016/j.steroids.2018.08.011DOI Listing
December 2018

[Sexual quality of life in patients with low back pain and the radiological changes evaluated in magnetic resonance].

Wiad Lek 2018;71(5):1039-1045

Zakład Diagnostyki Obrazowej, Szpital Chirurgii Urazowej W Piekarach Śląskich, Piekary Śląskie, Polska.

Objective: Introduction: Literature data concerning the association between the back pain syndrome and the quality of sexual life are rare, especially in the Polish literature. There are also no reports on the association between magnetic resonance (MRI) results and sexual satisfaction in patients with low back pain (L-S). The aim: To assess the association between the severity of degenerative-discopathic changes in the MRI of L-S spine and the quality of sexual life in patients with low back pain.

Patients And Methods: Materials and methods: The study involved 200 patients (107 women and 93 men), referred for MRI of the L-S spine due to the back pain syndrome. The assessment of satisfaction with sexual life at present and before the disease was made by the self-constructed questionnaire and with the use of the Question No. 8 of the Oswestry Questionnaire (ODI). In addition, the VAS (Visual Analogue Scale) was used. MRIs were analyzed based on the author's scoring scale, assessing selected radiological changes at levels L1-S1. The total score was in the range of 0-50 points.

Results: Results: There was a statistically significant decrease in the quality of sexual life (8.9 points vs 6.3 points) (<0.001). Back pain did not affect sexual life only in 36.9% of respondents. 26.5% patients were sexually inactive, 7.5% of them declared that pain was the reason for this. There was no statistically significant correlation between the intensity of radiological changes and satisfaction with sexual life.

Conclusion: Conclusions: Back pain affects the patients' sexual life. There was no association between the severity of degenerative-discopathic changes assessed in the MRI and the quality of sexual life in patients with L-S back pain syndrome.
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March 2019

Adipokine and cytokine levels in non-functioning adrenal incidentalomas (NFAI).

Endocr J 2018 Aug 5;65(8):849-858. Epub 2018 Jun 5.

Department of Endocrinology and Internal Medicine, Medical University of Gdansk, Gdansk, Poland.

Due to the fact that overweight or obesity is accompanied by hormonally active adrenal tumors: Cushing Syndrome-(CS) and Subclinical Cushing Syndrome (SCS), it is of high interest the correlation between different adipokines and cytokines secreted by adipose tissue, with metabolic disorders and hormonal activity in this group. Even in non-functioning adrenal incidentalomas (NFAI) elevated risk for cardiovascular disease and metabolic syndrome was demonstrated. The aim of the study was to investigate plasma adiponectin, leptin, resistin, tumor necrosis factor α (TNFα), interleukin 6 (IL6) and monocyte chemoattractant protein 1 (MCP1) levels in patients with NFAIs and healthy subjects. The study included 18 NFAI patients and 18 healthy subjects. The groups were homogeneous in terms of age, sex and body mass index (BMI). Patients with NFAI showed significantly higher circulating levels of pro-inflammatory cytokines compared to healthy controls (MCP 1: p < 0.001; TNFα p = 0.021; IL6 p = 0.012). On the other hand, adiponectin concentration was significantly lower in the NFAI group (p = 0.034). The serum leptin and resistin concentrations did not differ significantly between the two groups. Acquired results were not dependent on glucocorticoid and catecholamine secretion in NFAI patients. Also, there were no clear correlations between BMI and cytokine levels. It is possible that increased risk for cardiovascular and metabolic diseases reported in NFAI patients is at least partially dependent on adipose tissue activity.
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http://dx.doi.org/10.1507/endocrj.EJ18-0066DOI Listing
August 2018

[Patient with spine pain and magnetic resonance imaging result].

Wiad Lek 2018;71(2 pt 2):389-397

Katedra I Zakład Podstawowych Nauk Medycznych, Wydział Zdrowia Publicznego W Bytomiu, Śląski Uniwersytet Medyczny W Katowicach, Bytom, Polska.

Degenerative spine disease is a serious social problem. In most cases, it causes pain and neurological symptoms. Most patients are therefore referred for magnetic resonance imaging (MRI). The article discusses the relationship between back pain and magnetic resonance changes. The signification of some of the radiological symptoms remains controversial. Some of them are markers of acute pain, others may be clinically insignificant, occurring with age. Authors presents some of the magnetic resonance alterations and based on the latest articles discusses their clinical significance. The issues of performing routine, control MRI examination due to chronic back pain and the incidence of new radiological findings were also discussed.
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August 2018

Expression of adiponectin and leptin receptors in adrenal incidentaloma patients with subclinical hormone secretion.

Cancer Biomark 2018 ;22(2):325-332

Department of Endocrinology and Internal Medicine, Medical University of Gdansk, Poland.

Background: The role of adopokines in adrenal tumors' hormonal activity remains unclear. Obesity may induce arterial hypertension, disorders of carbohydrate metabolism, and is a risk factor of cardiovascular disease. In patients with subclinical hormone secretion by the adrenal cortex or medulla the risk of metabolic disease is increased.

Objective: Authors of this retrospective study selected 78 patients with subclinical hormone secretion out of all adrenal incidentaloma patients hospitalized in the Department of Endocrinology and Internal Medicine between 1995 and 2014.

Methods: The analyzed group comprised of 38 subclinical Cushing's syndrome (SCS), 40 incidentally discovered pheochromocytoma (PHEO) and 42 patients operated due to an adrenal tumor without pathological hormonal activity. Expression of adiponectin (AdipoR1, AdipoR2) and leptin (Ob-R) receptors in adrenal tumors was assessed in relation to body mass index (BMI) and hormonal activity.

Results: We found statistically significant negative correlations between BMI and expression of all examined receptors in SCS patients (AdipoR1: p= 0.032; AdipoR2: p< 0.001; leptin Ob-R: p= 0.001). In PHEOs, BMI correlated negatively only with AdipoR2 (p= 0.014).

Conclusions: Data obtained show that the most significant factor associated with the expression of AdipoR1, AdipoR2 and leptin Ob-R receptors in the adrenal tumor tissue is BMI, not their hormonal activity.
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http://dx.doi.org/10.3233/CBM-171049DOI Listing
October 2018

Contribution of activated beta3 integrin in the PDI release from endothelial cells.

Front Biosci (Landmark Ed) 2018 03 1;23:1612-1627. Epub 2018 Mar 1.

Department of Cytobiology and Proteomics, Medical University of Lodz, Poland,

Protein disulfide isomerase (PDI) is an abundant reticulum endoplasmic protein but also acts as an important functional regulator of some extracellular surface proteins. Recent studies suggest that PDI plays a role in integrin activation and thrombus formation. The aim of this study was to examine whether activation of integrin is the first stage leading to release of PDI from the subcellular compartments of endothelial cells to extracellular space. Our results show that endothelial cells which adhere to fibronectin or fibrinogen release significantly more PDI than those which adhere to poly-L-lysine. Cells treated with RGD peptide, Src and FAK kinase inhibitors and anti alphaVbeta3 antibody display lower PDI secretion. The destruction of the actin cytoskeleton of endothelial cells by cytochalasin D inhibits PDI release. When the endothelial cells adhere to fibrinogen or fibronectin, PDI and alphaVbeta3 gain free thiol groups. Our data suggest that upon activation of integrins, PDI is released from endothelial cells and forms a disulfide bond complex with alphaVbeta3 integrin.
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http://dx.doi.org/10.2741/4663DOI Listing
March 2018

Bilateral Tolosa-Hunt syndrome mimicking pituitary adenoma.

Endocrine 2017 Dec 14;58(3):582-586. Epub 2017 Oct 14.

Department of Endocrinology and Internal Medicine, Medical University of Gdańsk, Gdańsk, Poland.

The authors report a rare case of bilateral Tolosa-Hunt syndrome, which occurred in a 80-year-old female and remitted spontaneously. Inflammatory lesions were found not only in typical locations, i.e. superior orbital fissures and cavernous sinuses, but also in the pituitary; these imitated gland's macroadenoma in imaging studies.
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http://dx.doi.org/10.1007/s12020-017-1422-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5693964PMC
December 2017

Diagnostic and prognostic role of SF1, IGF2, Ki67, p53, adiponectin, and leptin receptors in human adrenal cortical tumors.

J Surg Oncol 2017 Sep 3;116(3):427-433. Epub 2017 Jul 3.

Department of Endocrinology and Internal Medicine, Medical University of Gdansk, Gdansk, Poland.

Background: The authors have examined the immunohistochemical expression of several proteins and their relationship with adrenal cortical carcinoma (ACC) diagnosis and progression.

Materials And Methods: A total of 83 patients with benign and malignant adrenal cortex tumors operated on in a single center were included in the study. Expression of the following proteins was examined: steroidogenic factor 1 (SF1), insulin growth factor 2 (IGF2), Ki67, p53, as well as adiponectin (Adipo R1, Adipo R2), and leptin (Ob-R) receptors.

Results: Multivariate analysis revealed that the expression of SF1, IGF2, and Adipo R1 and R2 receptors was associated with ACC diagnosis. An acknowledged proliferation marker Ki67 was related with the size of ACC and was an independent ACC diagnosis marker. The authors also assessed the relationship between immunohistochemical parameters and overall survival (OS) and disease progression. Only high IGF2 expression was associated with longer OS (P = 0.025). The most significant one for the prognosis of ACC patients was tumor resectability of the primary tumor. More favorable prognosis was found for young men (P = 0.033).

Conclusions: The presented data indicate that immunohistochemical assessment (of IGF2, SF1, Adipo R1, and R2 receptors' expression) may be useful in making the diagnosis of uncertain ACC cases.
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http://dx.doi.org/10.1002/jso.24665DOI Listing
September 2017

The Bony Bankart Lesion: How to Measure the Glenoid Bone Loss.

Pol J Radiol 2017 4;82:58-63. Epub 2017 Feb 4.

Department of Radiology, District Hospital of Orthopedics and Trauma Surgery, Piekary śląskie, Poland.

An osseous Bankart lesion is commonly seen in patients with an anterior shoulder dislocation. It is defined as a detachment of the anteroinferior labrum associated with a glenoid rim fracture. Radiological studies are crucial not only for detecting glenoid bone defects but also for measuring the amount of bone loss. The precise quantification of the bony defect is crucial for the therapeutic desicion-making and clinical outcomes. Although we know that major glenoid bone loss requires surgical intervention, none of the studies performed so far answered the question what size of the defect should be an indication for open surgery procedures. Moreover, there is still no consensus on the exact percentage of glenoid loss that results in a higher risk of re-dislocations. In our opinion, there is a strong need for a consensus on universally accepted measuring techniques of the glenoid defect as well as on algorithms with validated glenoid bone loss threshold values for therapeutic decision-making. In this study, we review the techniques described so far in the literature and try to assess if any of these techniques should be treated as a leading method of detecting and quantifying osseous glenoid lesions.
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http://dx.doi.org/10.12659/PJR.898566DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5304945PMC
February 2017

Adrenal incidentaloma in adults - management recommendations by the Polish Society of Endocrinology.

Endokrynol Pol 2016 ;67(2):234-58

Department of Internal Medicine and Endocrinology, Medical University of Warsaw.

Introduction: A wide use of imaging techniques results in more frequent diagnosis of adrenal incidenataloma.

Aim: To analyse the current state of knowledge on adrenal incidentaloma in adults in order to prepare practical management recommendations.

Methods: Following a discussion, the Polish Society of Endocrinology expert working group have analysed the available data and summarised the analysis results in the form of recommendations.

Imaging And Hormonal Assessment: Unenhanced adrenal computed tomography (CT) may be recommended as an initial assessment examination helpful in the differentiation between adenomas and "non-adenomatous" lesions. In the case of density > 10 Hounsfield units, CT with contrast medium washout assessment or MRI are recommended. However, in all patients with adrenal incidentaloma, hormonal assessment is recommended in order to exclude pheochromocytoma and hypercortisolism, notwithstanding the clinical picture or concomitant diseases. In addition, examination to exclude primary hyperaldosteronism is suggested in patients with diagnosed hypertension or hypokalaemia.

Treatment: Surgical treatment should be recommended in patients with adrenal incidentaloma, where imaging examinations suggest a malignant lesion (oncological indication) or with confirmed hormonal activity (endocrinological indication). The basis of the surgical treatment is laparoscopic adrenalectomy. Patients with suspected pheochromocytoma must be pharmacologically prepared prior to surgery. In patients not qualified for surgery, control examinations (imaging and laboratory tests) should be established individually, taking into consideration such features as the size, image, and growth dynamics of the tumour, clinical symptoms, hormonal tests results, and concomitant diseases.
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http://dx.doi.org/10.5603/EP.a2016.0039DOI Listing
March 2017

Right Aortic Arch with Aplasia of the Left Brachiocephalic Trunk Presented as Systolic Blood Pressure Difference Between Upper Extremities.

Pol J Radiol 2016 23;81:69-71. Epub 2016 Feb 23.

Department of Diagnostic Imaging, Hospital of Traumatology, Piekary Śląskie, Poland.

Background: The right aortic arch with mirror-image of branching arteries without coexisting congenital heart disease is a very rare anomaly.

Case Report: We report a case of the right-sided aortic arch with aplasia of the left brachiocephalic trunk in a 64-year-old women, presenting difference in systolic blood pressure between upper extremities. The history of the patient and angio-CT findings were described and visualized with images.

Conclusions: The knowledge of vascular variations is important for the clinical and therapeutic aspects.
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http://dx.doi.org/10.12659/PJR.895826DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4767319PMC
March 2016

Sunitinib treatment for multifocal renal cell carcinoma (RCC) and pancreatic neuroendocrine tumor (NET) in patient with Von Hippel-Lindau disease. Case Report.

Neuro Endocrinol Lett 2015 Dec;36(6):517-20

Department of Endocrinology and Internal Medicine, Medical University of Gdansk, Poland.

Objective: Von Hippel-Lindau disease (VHL) is an autosomal, dominant, hereditary disease occurring in approximately one in 36,000 births. VHL disease produces a variety of tumors and cysts in the central nervous system and visceral organs. Surgical management, when possible, improves prognosis and extends patient's life. When surgery is impossible, treatment with tyrosine kinase inhibitors demonstrates encouraging response rates.

Material And Method: We present a 60-year old patient with coexistence of multifocal renal cell carcinomas (RCC) and pancreatic neuroendocrine tumor (NET) in VHL disease, who received Sunitinib as the best option of treatment.

Results: Progression - free survival time is over 4 years. Regarding her acceptable tolerance for tyrosine kinase inhibitors, medical treatment is continued.

Conclusion: RCC and pancreatic NET associated with VHL are responsive to Sunitinib for prolonged periods of time. Tyrosine kinase inhibitors treatment for patients with multiple neoplasms associated with VHL disease may too be considered. Sunitinib showed acceptable toxicity.
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December 2015

Primary malignant lymphoma combined with clinically "silent" pheochromocytoma in the same adrenal gland.

World J Surg Oncol 2015 Sep 30;13:289. Epub 2015 Sep 30.

Department of Endocrinology and Internal Medicine, Medical University of Gdansk, ul. Dębinki 7, 80-288, Gdańsk, Poland.

An increased number of adrenal tumors are now diagnosed due to the increased number of abdominal CT scans being performed. We present the first case of malignant lymphoma combined with clinically "silent" pheochromocytoma in the same adrenal gland. An abdominal CT scan demonstrates unilateral adrenal lesion which suggests pheochromocytoma or adrenal carcinoma. Laboratory examinations revealed a slight increase of 24-h urine vanillylmandelic acid and 24-h urinary methanephrine excretion. Histological examination revealed two intermingled tumor cell proliferations-diffuse B cell lymphoma and pheochromocytoma.Unexpected coexistence of catecholamine-producing tumor with the other adrenal lesion can lead to serious complications of diagnosis and treatment. The adequate preparation for surgery can protect patient from threatening catecholamine crisis.
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http://dx.doi.org/10.1186/s12957-015-0711-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588683PMC
September 2015

Colon Necrosis Due to Sodium Polystyrene Sulfonate with and without Sorbitol: An Experimental Study in Rats.

PLoS One 2015 28;10(9):e0137636. Epub 2015 Sep 28.

Department of Medicine, SUNY Downstate Medical Center, Brooklyn, New York, United States of America.

Introduction: Based on a single rat study by Lillemoe et al, the consensus has been formed to implicate sorbitol rather than sodium polystyrene sulfonate (SPS) as the culprit for colon necrosis in humans treated with SPS and sorbitol. We tested the hypothesis that colon necrosis by sorbitol in the experiment was due to the high osmolality and volume of sorbitol rather than its chemical nature.

Methods: 26 rats underwent 5/6 nephrectomy. They were divided into 6 groups and given enema solutions under anesthesia (normal saline, 33% sorbitol, 33% mannitol, SPS in 33% sorbitol, SPS in normal saline, and SPS in distilled water). They were sacrificed after 48 hours of enema administration or earlier if they were very sick. The gross appearance of the colon was visually inspected, and then sliced colon tissues were examined under light microscopy.

Results: 1 rat from the sorbitol and 1 from the mannitol group had foci of ischemic colonic changes. The rats receiving SPS enema, in sorbitol, normal saline, distilled water, had crystal deposition with colonic necrosis and mucosal erosion. All the rats not given SPS survived until sacrificed at 48 h whereas 11 of 13 rats that received SPS in sorbitol, normal saline or distilled water died or were clearly dying and sacrificed sooner. There was no difference between sorbitol and mannitol when given without SPS.

Conclusions: In a surgical uremic rat model, SPS enema given alone or with sorbitol or mannitol seemed to cause colon necrosis and high mortality rate, whereas 33% sorbitol without SPS did not.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0137636PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4587365PMC
May 2016

Assessment of platelet function in endogenous hypercortisolism.

Endokrynol Pol 2015 ;66(3):207-13

Department of Endocrinology and Internal Medicine, Medical University of Gdańsk, Poland.

Introduction: It is commonly known that glucocorticoids exert a significant effect on haemostasis. Studies that have analysed the plasmatic coagulation system and fibrinolysis parameters in hypercortisolaemic patients are abundant. Platelet function, which plays a vital role in primary haemostasis, is much less clear in this context. We aimed at assessing platelet function in endogenous hypercortisolaemic patients.

Material And Methods: Twenty-five hypercortisolaemic patients were included in the study. Twelve of them were diagnosed with overt Cushing's syndrome (OCS) and 13 had subclinical Cushing's syndrome (SCS). Thirty healthy volunteers comprised the control group. In all subjects platelet function parameters were examined: ADP- and collagen-induced platelet aggregation (ADP-IPA and Col-IPA, respectively), IMPACT R (expressed as percentage of surface covered (SC) by platelets and average size (AS) of the adhering particles in μm2), as well as closure time (CT) after platelet activation with agonists: ADP and Col or Col and epinephrine (EPI). The statistical significance level was set at 0.05.

Results: There was no significant difference in mean values of ADP-IPA, Col-IPA, Col/Epi CT, Col/ADP CT, SC, and AS between hypercortisolaemic subjects and controls. No statistically significant differences in means of examined parameters were found between overt and subclinical Cushing's syndrome patients. Furthermore, no statistically significant relationships were found between these parameters and hormonal indicators of hypercortisolism: 24-hour urinary cortisol excretion, morning and evening serum cortisol level, and overnight-test cortisol concentration.

Conclusions: In hypercortisolaemic patients no primary haemostasis disorders are present, as reflected by platelet adhesion and ADP- and collagen-induced aggregation measurements.
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http://dx.doi.org/10.5603/EP.2015.0014DOI Listing
February 2017

Adrenocortical cancer (ACC) - literature overview and own experience.

Endokrynol Pol 2014 ;65(6):492-502

*Both co-authors contributed to the manuscript equally and both should be considered as 1st co-author Department of Endocrinology and Internal Medicine, Medical University of Gdansk, Gdansk, Poland Departments of Medicine and Endocrinology, King's College Hospital, London, UK Richard Dimbleby Department of Cancer Research, King's College London, Guy's Medical School Campus, London, UK.

Adrenocortical carcinoma (ACC) is a malignant endocrine tumour. The rarity of the disease has stymied therapeutic development. Age distribution shows two peaks: the first and fifth decades of life, with children and women more frequently affected. Although 60-70% of ACCs are biochemically found to overproduce hormones, it is not clinically apparent in many cases. If present, endocrine symptoms include signs of hypercortisolaemia, virilisation or gynaecomastia. ACC carries a poor prognosis, and a cure can be achieved only by complete surgical resection. Mitotane is used both as an adjuvant treatment and also in non-operative patients. The role of radio- and chemotherapy is still controversial. The post-operative disease free survival is low and oscillates around 30% due to high tumour recurrence rate. The diagnosis is based on tumour histological assessment with the use of the Weiss score, however urinary steroid profiling (if available) can serve to differentiate between ACC and other adrenal tumours. Conventional prognostic markers in ACC include stage and grade of disease, and, as currently reported, the presence of hypercortisolaemia. Molecular analysis has had a significant impact on the understanding of the pathogenetic mechanism of ACC development and the evaluation of prognostic and predictive markers, among which alterations of the IGF system, the Wnt pathway, p53 and molecules involved in cancer cell invasion properties and angiogenesis seem to be very promising. We here summarise our own experience related to the management of ACC and present a literature overview. We have not aimed to include a detailed summary of the molecular alterations biology described in ACC, as this has already been addressed in other papers.
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http://dx.doi.org/10.5603/EP.2014.0069DOI Listing
December 2016

The collection of five interesting cases of adrenal tumors from one medical center.

World J Surg Oncol 2014 Dec 8;12:377. Epub 2014 Dec 8.

Department of Endocrinology and Internal Medicine, Medical University of Gdansk, Gdansk, Dębinki St 80-288, Poland.

Introduction: Adrenal tumors are detected incidentally in 4 to 8% of patients in imaging studies. Adenomas, pheochromocytomas and adrenocortical carcinomas represent the most common tumors of the adrenal glands. Rarely are final histopathological reports are surprising.

Aim: The aim of our study is a retrospective analysis of selected clinical characteristics and hormonal studies in five cases of rare adrenal tumors.

Materials And Methods: We present five interesting cases of adrenal tumors: two medullary hyperplasia, one adenomatoid tumor, one hydatid cyst and a primary angiosarcoma of the adrenal gland. The final diagnosis was established by means of microscopic examination of the specimens.

Conclusions: The number of adrenal tumors was increased due to widespread use of imaging procedures. In patients without any known extra-adrenal malignancy most lesions are benign, non-hyper functioning adenomas. Adrenal tumors should be evaluated biochemically and radiologically.
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http://dx.doi.org/10.1186/1477-7819-12-377DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4295261PMC
December 2014

Recurrent acute suppurative thyroiditis due to pyriform sinus fistula in an adult--case report.

Endokrynol Pol 2013 ;64(3):234-6

Department of Endocrinology and Internal Medicine, Medical University of Gdansk, Poland.

We present a case study of acute suppurative thyroiditis (AST) with frequent recurrence due to anatomical malformation described as pyriform sinus fistula (PSF). Difficulty in diagnosis and treatment may be explained by the rarity of the disease in adult patients. AST had been observed in our patient five times before a radical surgery treatment was performed. The most typical symptoms and signs in our patient include: fever, difficulty in swallowing and tender tumour in the left side of the neck. Computed tomography (CT), barium swallow study and endoscopic examination gave us an opportunity to confirm the presence of an anatomical abnormality. After fistulectomy, we have not observed AST up to the date of this publication.
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January 2014