Publications by authors named "Ang Liu"

59 Publications

Spatial distribution, source analysis, and ecological risk assessment of polycyclic aromatic hydrocarbons (PAHs) in the sediments from rivers emptying into Jiaozhou Bay, China.

Mar Pollut Bull 2021 Apr 30;168:112394. Epub 2021 Apr 30.

Key Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China; Laboratory for Marine Ecology and Environmental Science, Qingdao Pilot National Laboratory for Marine Science and Technology, Qingdao 266071, China.

Sediment samples had been collected from five major rivers into Jiaozhou Bay, China to study the spatial distribution, sources, and ecological risks of 16 priority polycyclic aromatic hydrocarbons (PAHs) in April 2018. Results showed that the contents of PAHs in the sediments from the five rivers were significantly different. The distribution trend was Dagu < Yang < Licun < Baisha < Moshui River. The contents of PAHs in the eastern rivers were higher than those in the western portions. The contribution rate of HMW-PAHs was higher than LMW-PAHs in the sediments, with the majority of PAHs being four rings. PAHs in sediments were mainly derived from combustion sources, including the incomplete combustion of petroleum, coal, and wood. The benzo[a]pyrene toxic equivalent method (TEQ) showed that Moshui River had a high risk of carcinogenesis. The evaluation results of Effects Range Low(ERL), Effects Range Median (ERM) and Mean- Effects Range Median-quotient (M-ERM-Q) showed that PAHs in the sediments of Moshui River would occasionally have adverse biological effects, as well as possible medium and low ecological risks. The ecological risks of PAHs in the sediments of the other four rivers were low. Hence, we should focus on and control the input of PAHs in the Moshui River.
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http://dx.doi.org/10.1016/j.marpolbul.2021.112394DOI Listing
April 2021

sp. nov., a marine bacterium isolated from surface seawater of the Yellow Sea, and proposal of gen. nov., comb. nov. and comb. nov.

Int J Syst Evol Microbiol 2021 Apr;71(4)

Shandong Province Key Laboratory of Applied Mycology, School of Life Sciences, Qingdao Agricultural University, Qingdao 266109, PR China.

A bacterial strain designated RZ02 was isolated from surface seawater collected from the Yellow Sea in PR China and characterized by polyphasic taxonomy. Cells of strain RZ02 were Gram-stain-negative, aerobic, non-motile, catalase- and oxidase-positive rods forming ochre-pigmented colonies. Growth occurred at 7-36 °C (optimum, 30 °C), at pH 6.0-9.0 (optimum, pH 7.0) and with 1-5 % (optimum, 2 %) NaCl. The major cellular fatty acids (>10 %) of strain RZ02 were summed feature 8 (C6 and/or C7), summed feature 3 (C7 and/or C6) and C. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol and sphingoglycolipid. The genome size of strain RZ02 was 2.79 Mbp with a G+C content of 55.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain RZ02 was mostly related to SW-109 and HDW-31 (97.3 and 97.1% sequence similarity, respectively), and formed a phyletic lineage with members of the genus . The phylogenetic analysis based on the up-to-date bacterial core gene sequences confirmed that strain RZ02 clustered within the genus . The average nucleotide identity and DNA-DNA hybridization values between strain RZ02 and SW-109 and HDW-31 were 72.8 and 72.9 % and 18.7 and 18.5%, respectively. Based on these evidences, strain RZ02 is proposed to represent a novel species of the genus under the name sp. nov. The type strain is RZ02 (=KCTC 62828=MCCC 1K04521). In addition, based on the results of whole genome analyses, proposals of gen. nov., comb. nov. and comb. nov. are also included.
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http://dx.doi.org/10.1099/ijsem.0.004780DOI Listing
April 2021

Reclassification of Algibacter wandonensis as a Later Heterotypic Synonym of Algibacter lectus Based on Whole-Genome Sequence Analysis.

Curr Microbiol 2021 Mar 1;78(3):1034-1038. Epub 2021 Feb 1.

Department of Pathogenic Biology, College of Basic Medicine, Jining Medical University, Jining, Shandong, 272067, P. R. China.

The genus Algibacter belongs to the family Flavobacteriaceae of the Bacteroidetes, and all members of this genus were isolated from marine environments. Among the Algibacter species, two members, Algibacter lectus KMM 3902 and Algibacter wandonensis WS-MY22, were isolated from green algae and sediment around a brown algae respectively. The 16S rRNA gene sequences of these two type strains possess 99.4% sequence similarity. In this study, further studies were undertaken to clarify the taxonomic assignments of the two species. Whole-genome sequence analysis showed that the similarities for other phylogenetic markers are also very high (i.e. 99.9% for gyrB, 99.6% for recA and 99.9% for rpoD). Average nucleotide identity, average amino acids identity and digital DNA-DNA hybridization value between A. lectus KMM 3902 and A. wandonensis WS-MY22 are 98.3%, 98.6% and 89.4% respectively, all clearly exceed suggested species delineation thresholds. Furthermore, phylogenetic trees based on sequences of 16S rRNA gene and up-to-date bacterial core gene set (UBCG) consisting of 92 genes provided additional evidence that A. lectus KMM 3902 and A. wandonensis WS-MY22 are very closely related. In addition, a review of their profiles indicated that A. lectus KMM 3902 and A. wandonensis WS-MY22 did not present pronounced differences at phenotypic and chemotaxonomic levels. Based on these evidence, we propose that A. wandonensis should be reclassified as later heterotypic synonyms of A. lectus.
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http://dx.doi.org/10.1007/s00284-021-02356-5DOI Listing
March 2021

Distribution, sources, and risk assessment of polycyclic aromatic hydrocarbons (PAHs) in surface sediments from Daya Bay, South China.

Environ Sci Pollut Res Int 2021 Jan 21. Epub 2021 Jan 21.

Marine Bioresources and Environment Research Center, First Institute of Oceanography, Ministry of Natural Resources, Qingdao, 266061, China.

In this study, the concentrations of 16 kinds of polycyclic aromatic hydrocarbons (PAHs) in surface sediments of Daya Bay were determined and analyzed. Results showed that 16 PAHs were detected in all the samples, and the total PAH concentration ranged from 70.18 to 128.04 ng g, with an average of 103.17 ng g. The cyclic number distribution of PAHs in the sediments was mainly 4 and 5 rings. Six classic PAH ratios named Ant/(Ant + Phe), Fla/(Fla + Pyr), [InP/(InP + BghiP)], [BaA/(BaA + Chr)], BaA/BghiP, and LMW/HMW, and principal component analyses showed that the main source of PAHs in this region was combustion (biomass, coal, and petroleum combustion), and the secondary source was petroleum. The ecological risk analysis of PAHs by using effect range low/median method and mean effects range-median quotient method showed that all of PAHs are lower than the effect range low (ERL) level and the effects range-median quotient (M-ERM-Q) value of all stations is 0.0027-0.0067, with an average value of 0.0046. Thus, it can be seen that PAHs are at a low-risk level in surface sediments of Daya Bay.
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http://dx.doi.org/10.1007/s11356-020-11956-wDOI Listing
January 2021

Zongyanglinia huanghaiensis gen. nov., sp. nov., a novel denitrifying bacterium isolated from the yellow sea, and transfer of Pelagicola marinus to Zongyanglinia gen. nov. as Zongyanglinia marinus comb. nov.

Antonie Van Leeuwenhoek 2021 Feb 3;114(2):137-149. Epub 2021 Jan 3.

Shandong Province Key Laboratory of Applied Mycology, School of Life Sciences, Qingdao Agricultural University, Qingdao, 266109, People's Republic of China.

Two Gram-stain-negative, oxidase- and catalase-positive, aerobic strains (CY05 and H18S-6) were isolated from sediment samples of the Yellow Sea, China. The strains were positive for denitrification. Optimum growth was observed at 20 °C, pH 7.5-8.0 and with 2.0%-3.0% NaCl. The predominant cellular fatty acids (> 10%) were summed feature 8 (C ω7c and/or C ω6c), major respiratory quinone was ubiquinone-10 and main polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid and one unidentified aminolipid. The approximate genome size of strains CY05 and H18S-6 were 4.86 and 5.04 Mbp, the genomic G + C content of them were 54.2 and 54.5%, respectively. Both of the phylogenetic analysis based on 16S rRNA gene sequences and the up-to-date bacterial core gene (UBCG) sequences revealed that strains CY05, H18S-6 and Pelagicola marinus DSW4-44 formed a distinct monophyletic clade within the family Rhodobacteraceae. The ANI and isDDH values between strains CY05 and H18S-6 were 94.0% and 56.5%, between CY05 and Pelagicola marinus DSW4-44 were 94.1% and 59.8%, respectively, all below the accepted threshold value for species delineation. But the ANI and isDDH values between strains H18S-6 and Pelagicola marinus DSW4-44 were 96.8% and 76.7% respectively, indicating that strains H18S-6 and Pelagicola marinus DSW4-44 belong to the same species. Based on the distinctive polyphasic evidence, CY05 represent a novel species of a novel genus of the family Rhodobacteraceae, for which the name Zongyanglinia huanghaiensis gen. nov., sp. nov. is proposed. The type strain is CY05 (= MCCC 1K04409 = KCTC 62200). Moreover, the reclassification of Pelagicola marinus Choi et al. 2019 as Zongyanglinia marinus comb. nov. (type strain DSW4-44 = KCTC 62762 = KCCM 43261 = JCM 33637) is proposed based on the polyphasic taxonomic data obtained in this study.
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http://dx.doi.org/10.1007/s10482-020-01507-1DOI Listing
February 2021

Polycyclic aromatic hydrocarbon (PAH) distribution in surface sediments from Yazhou Bay of Sanya, South China, and their source and risk assessment.

Mar Pollut Bull 2021 Jan 7;162:111800. Epub 2020 Nov 7.

Marine Bioresources and Environment Research Center, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China; Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China.

The distribution, source, and ecological risk of polycyclic aromatic hydrocarbons (PAHs) in surface sediments were analyzed and discussed at 15 sampling sites in Yazhou Bay, Sanya. Results showed that the total PAH content in the surface sediments of Yazhou Bay ranged from 13.20 ng/g to 40.37 ng/g, and the average content was 31.53 ng/g. Component analysis showed that the distribution of various PAHs in the surface sediments of Yazhou Bay was relatively average, and 3- and 4-ring PAHs were the dominant species. PAHs were traced by characteristic ratio method and principal component analysis. PAHs in the surface sediments mainly came from combustion sources, including incomplete combustion of petroleum, coal, and biomass, which also indicated the existence of petroleum sources. The ecological risk assessment results of surface sediments showed that, compared with effect range low and effect range median (ERM) of the ecological risk of 14 kinds of PAHs detected, no ecological risk existed for PAHs of all sampling sites in Yazhou Bay. The mean ERM quotient analysis also showed that PAHs in the sediments in Yazhou Bay were at a low risk level.
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http://dx.doi.org/10.1016/j.marpolbul.2020.111800DOI Listing
January 2021

Population Pharmacokinetic Analysis of BMS-986166, a Novel Selective Sphingosine-1-Phosphate-1 Receptor Modulator, and Exposure-Response Assessment of Lymphocyte Counts and Heart Rate in Healthy Participants.

Clin Pharmacol Drug Dev 2021 Jan 8;10(1):8-21. Epub 2020 Oct 8.

Bristol Myers Squibb, Princeton, New Jersey, USA.

Sphingosine-1-phosphate (S1P) binding to the S1P-1 receptor (S1P1R) controls the egress of lymphocytes from lymphoid organs and targets modulation of immune responses in autoimmune diseases. Pharmacologic modulation of S1P receptors has been linked to heart rate reduction. BMS-986166, a prodrug of the active phosphorylated metabolite BMS-986166-P, presents an improved cardiac safety profile in preclinical studies compared to other S1P1R modulators. The pharmacokinetics, safety, and pharmacodynamics of BMS-986166 versus placebo after single (0.75-5.0 mg) and repeated (0.25-1.5 mg/day) oral administration were assessed in healthy participants after a 1-day lead-in placebo period. A population model was developed to jointly describe BMS-986166 and BMS-986166-P pharmacokinetics and predict individual exposures. Inhibitory sigmoid models described the relationships between average daily BMS-986166-P concentrations and nadir of time-matched (day -1) placebo-corrected heart rate on day 1 (nDDHR, where DD represents ∆∆) and nadir of absolute lymphocyte count (nALC). Predicted decreases in nDDHR and nALC were 9 bpm and 20% following placebo, with maximum decreases of 10 bpm in nDDHR due to drug effect, and approximately 80% in nALC due to drug and placebo. A 0.5-mg/day dose regimen achieves the target 65% reduction in nALC associated with a 2-bpm decrease in nDDHR over placebo.
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http://dx.doi.org/10.1002/cpdd.878DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7821288PMC
January 2021

Composition, content, source, and risk assessment of PAHs in intertidal sediment in Shilaoren Bay, Qingdao, China.

Mar Pollut Bull 2020 Oct 31;159:111499. Epub 2020 Jul 31.

Marine Bioresources and Environment Research Center,First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061,China; Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China. Electronic address:

Intertidal sediment samples from Shilaoren Bay, Qingdao, China, were collected to determine the concentrations and compositions of 16 polycyclic aromatic hydrocarbons (PAHs). The sources of PAHs were analyzed, and risk assessment was performed. Results show that the pollution level of PAHs in the investigated area was low. The majority of PAHs are four-ring PAHs. The PAHs in this region were mainly derived from combustion sources, including the incomplete combustion of petroleum, coal, and biomass, such as vegetation, which were affected to some extent by petroleum sources. The highest concentration of PAHs in all sampling sites was lower than that of effects range low, and the mean effects range median quotient was low. Therefore, the PAHs in the study area did not have toxic and side effects on the ecological environment, and ecological risk was low.
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http://dx.doi.org/10.1016/j.marpolbul.2020.111499DOI Listing
October 2020

sp. nov., a marine bacterium isolated from intertidal sand of the Yellow Sea.

Int J Syst Evol Microbiol 2020 May;70(5):3497-3503

Department of Pathogenic Biology, Jining Medical University, Jining 272067, Shandong, PR China.

A novel rod-shaped and Gram-stain-negative bacterium, designated strain RZ05, was isolated from a sand sample collected from the intertidal zone of the Yellow Sea, PR China. Results of phylogenetic analysis based on 16S rRNA gene sequences revealed that strain RZ05 clusters within the genus , a member of the family , and has the highest sequence similarity to KCTC 22021 (97.8 %), followed by KCTC 52191 (97.2 %). Cells of this strain were observed to be aerobic, oxidase- and catalase-positive, motile by gliding and formed yellow colonies. Growth occurred at 7-40 °C (optimum, 30 °C), at pH 6.5-9.5 (optimum, pH 7.0) and with 0.5-6 % (optimum, 2 %) NaCl. Its polar lipid profile included phosphatidylethanolamine, two unidentified glycolipids, one unidentified aminolipid and four unidentified lipids. The major cellular fatty acids were iso-C, iso-C G, iso-C 3-OH, iso-C 3-OH, iso-C 3-OH, summed feature 9 (10-methyl C/iso-Cω9) and summed feature 3 (iso-C 2-OH/Cω7/Cω6). The only respiratory quinone was menaquinone 6 (MK-6). The genome of strain RZ05 was 4.65 Mbp with a G+C content of 38.9 mol%. The average nucleotide identity and DNA-DNA hybridization values between strain RZ05 and its most closely related type strain KCTC 22021 were 80.3 and 26.3  %, respectively. The results of phylogenetic, phenotypic and chemotaxonomic analyses indicated that strain RZ05 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RZ05 (=KCTC 62834=MCCC 1K03617).
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http://dx.doi.org/10.1099/ijsem.0.004206DOI Listing
May 2020

Whole genome analysis calls for a taxonomic rearrangement of the genus Colwellia.

Antonie Van Leeuwenhoek 2020 Jul 26;113(7):919-931. Epub 2020 Mar 26.

Department of Pathogenic Biology, Jining Medical University, Jining, 272067, Shandong, People's Republic of China.

Microbial taxonomy is the foundation of microbiology and rapid advancements in DNA sequencing technologies are providing new approaches to address prevailing questions in this field. The family Colwelliaceae, which currently comprises four genera, is a diverse and globally abundant group of Gamaproteobacteria. Based on 14 publically available genomes of bacteria strains labeled as members of the family Colwelliaceae, phylogenomic analyses were conducted to revisiting the taxonomic status of this family both in the genus and species level. Using genome-based phylogeny as a primary guideline and genome-based similarity indexes including average amino acid identity, percentage of conserved proteins, average nucleotide identity, and the digital DNA-DNA hybridization as supplements, the following taxonomic proposals were proposed: Colwellia polaris, Colwellia beringensis, Colwellia sediminilitoris, Colwellia aestuarii, Colwellia chukchiensis and Colwellia mytili should be reclassified into the novel genus Cognaticolwellia; Colwellia agarivorans should be reclassified into the novel genus Pseudocolwellia. Our results constitute a solid framework for current and future taxonomic decisions within this family, which will be helpful for avoiding confusion with ecological and evolutionary interpretations in subsequent studies.
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http://dx.doi.org/10.1007/s10482-020-01405-6DOI Listing
July 2020

Correction: Bis(ethylmaltolato)oxidovanadium(iv) inhibited the pathogenesis of Alzheimer's disease in triple transgenic model mice.

Metallomics 2020 04;12(4):631

Shenzhen Key Laboratory of Marine Biotechnology and Ecology, College of Life Sciences and Oceanography, Shenzhen University, 518060 Shenzhen, China.

Correction for 'Bis(ethylmaltolato)oxidovanadium(iv) inhibited the pathogenesis of Alzheimer's disease in triple transgenic model mice' by Zhijun He et al., Metallomics, 2020, DOI: 10.1039/c9mt00271e.
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http://dx.doi.org/10.1039/d0mt90008gDOI Listing
April 2020

Safety, pharmacokinetics and pharmacodynamics of branebrutinib (BMS-986195), a covalent, irreversible inhibitor of Bruton's tyrosine kinase: Randomised phase I, placebo-controlled trial in healthy participants.

Br J Clin Pharmacol 2020 09 12;86(9):1849-1859. Epub 2020 Apr 12.

Bristol Myers Squibb, Princeton, NJ, USA.

Aims: Branebrutinib (BMS-986195) is a potent, highly selective, oral, small-molecule, covalent inhibitor of Bruton's tyrosine kinase (BTK). This study evaluated safety, pharmacokinetics and pharmacodynamics of branebrutinib in healthy participants.

Methods: This double-blind, placebo-controlled, single- and multiple-ascending dose (SAD; MAD) Phase I study (NCT02705989) enrolled participants into 3 parts: SAD, MAD and JMAD (MAD in first-generation Japanese participants). In each part, participants were randomised 3:1 to receive branebrutinib (SAD: 0.3-30 mg; [J]MAD: 0.3-10 mg) or placebo. Participants in the MAD parts received branebrutinib daily for 14 days and were followed for 14 days postdosing. Safety was assessed by monitoring, laboratory and physical examinations, vital signs, and recording adverse events (AEs). Pharmacodynamics were assessed with a mass spectrometry assay that measured drug-occupied and free BTK.

Results: The SAD, MAD and JMAD parts of the study included 40, 32 and 24 participants. Branebrutinib was well tolerated and AEs were mild/moderate, except for 1 serious AE that led to discontinuation. Branebrutinib was rapidly absorbed, with maximum plasma concentration occurring within 1 hour and a half-life of 1.2-1.7 hours, dropping to undetectable levels within 24 hours. BTK occupancy was rapid, with 100% occupancy reached after a single 10-mg dose. BTK occupancy decayed predictably over time (mean half-life in MAD panels: 115-154 hours), such that pharmacodynamic effects were maintained after branebrutinib plasma levels fell below the lower limit of quantification.

Conclusion: Rapid and high occupancy of BTK and the lack of notable safety findings support further clinical development of branebrutinib.
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http://dx.doi.org/10.1111/bcp.14290DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7444767PMC
September 2020

Bis(ethylmaltolato)oxidovanadium(iv) inhibited the pathogenesis of Alzheimer's disease in triple transgenic model mice.

Metallomics 2020 04 23;12(4):474-490. Epub 2020 Jan 23.

Shenzhen Key Laboratory of Marine Biotechnology and Ecology, College of Life Sciences and Oceanography, Shenzhen University, 518060 Shenzhen, China.

Vanadium compounds have been reported to mimic the anti-diabetes effects of insulin on rodent models, but their effects on Alzheimer's disease (AD) have rarely been explored. In this paper, 9-month-old triple transgenic AD model mice (3×Tg-AD) received bis(ethylmaltolato)oxidovanadium(iv) (BEOV) at doses of 0.2 mmol L (68.4 μg mL) and 1.0 mmol L (342 μg mL) for 3 months. BEOV at both doses was found to improve contextual memory and spatial learning in AD mice. It also improved glucose metabolism and protected neuronal synapses in the AD brain, as evidenced respectively by F-labeled fluoro-deoxyglucose positron emission tomography (F-FDG-PET) scanning and by transmission electron microscopy. Inhibitory effects of BEOV on β-amyloid (Aβ) plaques and neuronal impairment in the cortex and hippocampus of fluorescent AD mice were visualized three-dimensionally by applying optical clearing technology to brain slices before confocal laser scanning microscopy. Western blot analysis semi-quantitatively revealed the altered levels of Aβ in the brains of wildtype, AD, and AD treated with 0.2 and 1.0 mmol L BEOV mice (70.3%, 100%, 83.2% and 56.8% in the hippocampus; 82.4%, 100%, 66.9% and 42% in the cortex, respectively). The mechanism study showed that BEOV increased the expression of peroxisome proliferator-activated receptor γ (PPARγ) (140%, 100%, 142% and 160% in the hippocampus; 167%, 100%, 124% and 133% in the cortex) to inactivate the JAK2/STAT3/SOCS-1 pathway and to block the amyloidogenesis cascade, thus attenuating Aβ-induced insulin resistance in AD models. BEOV also reduced protein tyrosine phosphatase 1B (PTP1B) expression (74.8%, 100%, 76.5% and 53.8% in the hippocampus; 71.8%, 100%, 94.2% and 81.8% in cortex) to promote insulin sensitivity and to stimulate the PI3K/Akt/GSK3β pathway, subsequently reducing tau hyperphosphorylation (phosphorylated tau396 levels were 51.1%, 100%, 56.1% and 50.2% in the hippocampus; 22.2%, 100%, 36.1%, and 24% in the cortex). Our results suggested that BEOV reduced the pathological hallmarks of AD by targeting the pathways of PPARγ and PTP1B in 3×Tg AD mice.
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http://dx.doi.org/10.1039/c9mt00271eDOI Listing
April 2020

Comprehensive analysis of differentially expressed microRNAs and mRNAs in MDBK cells expressing bovine papillomavirus oncogene.

PeerJ 2019 22;7:e8098. Epub 2019 Nov 22.

College of Animal Science and Technology, Hainan University, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Hainan University, Haikou, China.

Delta bovine papillomaviruses (δBPVs) causes fibropapillomas or bladder cancer in cattle. is the major oncogene of δBPVs; however, the influence that oncogene has on host microRNA (miRNA) and mRNA expression profiles remains little elucidated. In the present study, small RNA sequencing and RNA sequencing were used to explore alterations in miRNAs and mRNAs in over-expressing Madin-Darby bovine kidney (MDBK) cells compared with controls. In total, 77 miRNAs (including 30 bovine-derived miRNAs) and 223 genes were differentially expressed (DE) following overexpression. The dysregulated genes were mainly involved in metabolic and biosynthetic processes. We constructed a potential miRNA-gene regulatory network from the differentially expressed genes (DEGs) and DE miRNAs. Finally, 22 DEGs and nine DE miRNAs were selected for RT-qPCR validation. Of these, downregulation of six miRNAs, , , , , , and were confirmed; In addition, upregulation of 16 genes, , , , , , , , , , , , , , , , and and downregulation of two genes, and were confirmed. Specially, and potentially regulated and , which were involved in cancer progression and angiogenesis. Our study presented for the first time the comprehensive miRNA and mRNA alterations in MDBK cells expressing the BPV oncogene, providing new insights into the tumorigenesis induced by BPV E5.
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http://dx.doi.org/10.7717/peerj.8098DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6876490PMC
November 2019

Concentration level, distribution model, source analysis, and ecological risk assessment of polycyclic aromatic hydrocarbons in sediments from laizhou bay, China.

Mar Pollut Bull 2020 Jan 8;150:110690. Epub 2019 Nov 8.

Key Laboratory for Marine Bioactive Substances and Modern Analytical Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao, 266061, China; Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, China. Electronic address:

To master the distribution patterns and environmental risk of 16 USEPA preferential polycyclic aromatic hydrocarbons (PAHs) in surface sediments from Laizhou Bay, 20 samples were collected and investigated in this survey. The average PAH concentration in these sediments ranged from 268.97 ng/g to 895.37 ng/g with an average of 612.52 ng/g, thereby suggesting a relatively low PAH pollution in Laizhou Bay compared with other bays in the world. Tricyclic PAHs account for 79% of the total PAH content and were eventually identified as the most crucial component of these sediments. The PCA-MLR results identify fossil fuel combustion, biomass burning, and coke oven as the main sources of PAHs that account for 26.69%, 67.16%, and 6.15% of the total PAH concentration in the collected sediments, respectively. The effect range low/effect range median (ERL/ERM) reveal the low toxicity of PAHs in these sediments. However, the concentration of Fle at each survey site exceeds the ERL level. Meanwhile, the mean effects range-median quotient (M-ERM-Q) indicates the low level of ecological risk of PAHs in the surface sediments from Laizhou Bay. However, the contingency risk of Fle and Phe cannot be ruled out.
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http://dx.doi.org/10.1016/j.marpolbul.2019.110690DOI Listing
January 2020

Litorilituus lipolyticus sp. nov., isolated from intertidal sand of the Yellow Sea in China, and emended description of Colwellia asteriadis.

Antonie Van Leeuwenhoek 2020 Apr 7;113(4):449-458. Epub 2019 Nov 7.

Department of Pathogenic Biology, Jining Medical University, Jining, 272067, Shandong, People's Republic of China.

A Gram-stain negative, rod-shaped, aerobic, oxidase-positive and catalase-weakly positive bacterial strain with polar or subpolar flagellum, designated RZ04, was isolated from an intertidal sand sample collected from a coastal area of the Yellow Sea, China. The organism was observed to grow optimally at 25 °C and pH 6.5-7.0 with 2% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain RZ04 was closely related to Colwellia asteriadis (similarity 96.9%) and Litorilituus sediminis (similarity 96.8%), and 94.4-96.4% sequence similarities to other type strains of species of the genera belonged to the family Colwelliaceae. The dominant fatty acids of strain RZ04 were determined to be Cω8c, Cω8c, C and summed feature 3 (Cω6c and/or Cω7c), and the predominant isoprenoid quinone was determined to be quinone 8 (Q-8). Phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminophospholipid and four unidentified lipids were determined to be the major constituents of the polar lipids. The genome of strain RZ04 is 4.14 Mbp with a G + C content of 37.4 mol%. A total of 3631 genes are predicted, with 3531 protein-coding genes, 75 RNA genes and 25 pseudogenes. Based on phenotypic, genotypic and phylogenetic analysis, strain RZ04 is considered to represent a novel species in the genus Litorilituus, for which the name Litorilituus lipolyticus is proposed. The type strain is RZ04 (= MCCC 1K03616 = KCTC 62835). An emended description of Colwellia asteriadis is also provided.
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http://dx.doi.org/10.1007/s10482-019-01355-8DOI Listing
April 2020

EB virus-positive tumors are inhibited by rBCG expressing hGM-CSF and LMP2A.

Hum Vaccin Immunother 2020 03 29;16(3):654-663. Epub 2019 Oct 29.

School of Basic Medical, Jining Medical University, Shandong, China.

For the development of safe and effective EBV (Epstein-Barr virus) vaccines, the Ag85A signal peptide from H37Rv was used to construct a recombinant secretory BCG (Bacillus Chalmette-Guérin) plasmid. The Ag85A gene, fused to the EBV LMP2A (latent membrane protein) and hGM-CSF (human granulocyte/macrophage colony-stimulating factor) genes, was inserted into the pMV261 vector (secretory BCG plasmid). The expression levels of the hGM-CSF and LMP2A proteins in rBCG (recombinant BCG) were measured by Western blot analysis. Humoral immunity, cellular immunity, and antitumor effects were determined by a series of experiments. The recombinant pMVGCA plasmid effectively expressed GCA (hGM-CSF and LMP2A fusion protein) in BCG after transformation, and the rBCG proteins were recognized by antibodies against hGM-CSF and LMP2A. Six weeks after immunization, the maximum dose of rBCG resulted in antibody titers of 1:19,800 (hGM-CSF antibody) and 1:21,800 (LMP2A antibody). When the effector:target ratio was 40:1, specific lysis was maximal and approximately two times stronger than that in mice immunized with the control. Tumorigenicity was lower in the rBCG treatment group, with a tumor inhibition rate of 0.81 ± 0.09 compared with the control groups. EB virus-positive tumors are inhibited by rBCG expressing an hGM-CSF and LMP2A fusion protein.
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http://dx.doi.org/10.1080/21645515.2019.1670593DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7227694PMC
March 2020

Use of acoustic emission to evaluate the micro-mechanical behavior of sands in single particle compression tests.

Ultrasonics 2019 Nov 13;99:105962. Epub 2019 Jul 13.

Department of Geotechnical Engineering, College of Civil Engineering, Tongji University, Shanghai 200092, China.

Particle breakage has been recognized as a crucial factor affecting the mechanical behavior of stressed granular assemblages. To understand such underlying micro-mechanical behavior, Acoustic Emission (AE) technique that is capable of continuously diagnosing the deterioration and failure process of stressed materials was employed into single particle compression tests on silica sands. Regardless of different particle sizes, the fracturing process could be highly featured by AE characteristics, in which AE hit rate and peak frequency characteristics were analyzed to evaluate the intensity and mode of micro-mechanical behaviors, respectively. "Early warning omens" regarding the impending failure of the stressed particle is revealed in terms of the initiation and rapid increase of high-frequency AE components, as well as the rapid increase of AE hit rate. The effect of "prehistory of failure" on the stressed particle is sensitively featured by the highly emitted AE events after the catastrophic failure. Furthermore, a frequency-based method is suggested to distinguish different modes of micro-mechanical behaviors associated with particle readjustment, asperity abrasion, and microcracking. Further employment of the present result is expected to continuously evaluate the intensity and mode of particle interactions in stressed granular assemblages.
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http://dx.doi.org/10.1016/j.ultras.2019.105962DOI Listing
November 2019

Design of a low-crosstalk half-wavelength pitch nano-structured silicon waveguide array.

Opt Lett 2019 Jul;44(13):3266-3269

An ultra-compact half-wavelength pitch silicon waveguide array with very low crosstalk is proposed and analyzed in this work. We first show the design of a pair of low-crosstalk silicon waveguides with only half-wavelength spacing, where the placement of two thin silicon strips asymmetrically in between the waveguides is key to having very low crosstalk. We next extend this nano-structured two-waveguide design to form a low-crosstalk half-wavelength pitch silicon waveguide array. Coupled-mode theory shows that, for an array length of 1 mm, the insertion loss of the input waveguide is as low as -0.13  dB for the TE-like mode at 1550 nm, and the crosstalk in all other waveguides remains below about -18  dB. This half-wavelength pitch waveguide array also exhibits a favorable fabrication error tolerance when taking into account the waveguide width variations in practice. It offers a promising platform for realization of integrated optical phased arrays for solid-state lidars with a large field of view.
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http://dx.doi.org/10.1364/OL.44.003266DOI Listing
July 2019

Flavivirga rizhaonensis sp. nov., a marine bacterium isolated from intertidal sand.

Antonie Van Leeuwenhoek 2019 Nov 19;112(11):1645-1653. Epub 2019 Jun 19.

Department of Pathogenic Biology, Jining Medical University, Jining, 272067, Shandong, People's Republic of China.

A bacterial strain designated RZ03 was isolated from an intertidal sand sample from the Yellow Sea in China and characterised using a polyphasic taxonomic approach. Cells of strain RZ03 were observed to be Gram-stain negative, aerobic, and oxidase and catalase positive rods showing gliding motility and forming yellow colonies. Growth was found to occur at 7-30 °C (optimum, 25 °C), at pH 5.5-9.5 (optimum, pH 6.5-7.0) and with 0.5-5% NaCl (optimum, 1.5-2%). Phylogenetic analysis based on 16S rRNA gene sequences indicates that strain RZ03 clusters within members of the genus Flavivirga of the family Flavobacteriaceae and is closely related to the type strains Flavivirga amylovorans JCM 17112 and Flavivirga jejuensis JCM 17113 (97.9% and 97.5% similarity, respectively). The predominant cellular fatty acids are iso-C, iso-C G, iso-C 3-OH and iso-C 3-OH and the major respiratory quinone is MK-6. Polar lipids include phosphatidylethanolamine, three unidentified aminolipids, an unidentified phospholipid and four unidentified lipids. The genome of strain RZ03 is 4.88 Mbp with a G+C content of 32.2 mol%. A total of 4152 genes are predicted, with 4052 protein-coding genes, 51 RNA genes and 49 pseudogenes. This polyphasic study suggests that strain RZ03 represents a novel species in the genus Flavivirga, for which the name Flavivirga rizhaonensis is proposed. The type strain is RZ03(= KCTC 62833 = MCCC 1K03615).
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http://dx.doi.org/10.1007/s10482-019-01291-7DOI Listing
November 2019

High performance ultra-compact SOI waveguide crossing.

Opt Express 2018 Oct;26(20):25602-25610

Waveguide crossing is an important integrated photonic component that will be routinely used for high-density and large-scale photonic integrated circuits, such as optical switches and routers. Several techniques have been reported in achieving high performance waveguide crossings on a silicon-on-insulator photonic platform, i.e., low-loss and low-crosstalk waveguide crossings based on multimode interference, bi-layer tapering, optical transformation, metamaterials, and subwavelength gratings. Until recently, not much attention has been given to the reduction of the footprint of waveguide crossings. Here we experimentally demonstrate an ultra-compact waveguide crossing on silicon photonic platform with a footprint only ~1 × 1 μm. Our simulations show that it has a low insertion loss (< 0.175 dB) and low crosstalk (< -37dB) across the whole C-band, while the fabricated one has an insertion loss < 0.28 dB and crosstalk around -30 dB for the C-band.
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http://dx.doi.org/10.1364/OE.26.025602DOI Listing
October 2018

Downregulation of peroxiredoxin II suppresses the proliferation and metastasis of gastric cancer cells.

Oncol Lett 2018 Oct 25;16(4):4551-4560. Epub 2018 Jul 25.

Department of Interventional Radiology, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221002, P.R. China.

Peroxiredoxin (Prx) II is an imperative member of the superfamily of peroxidases. It serves an essential role in scavenging organic hydroperoxide and HO. It is involved in the development of various malignant tumors. In order to investigate the significance of Prx II expressions level in gastric cancer (GC), downregulation of Prx II was performed to investigate its role in the proliferation and migration of gastric adenocarcinoma cells. In GC cells and 45 GC specimens, the mRNA and protein expression levels of Prx II were determined using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blot analysis, respectively. The Prx II expression profile in another 116 GC specimens was also detected with immunohistochemistry (IHC). The changes in the proliferation and migration of MKN45 and MGC-803 cells folllowing transfection with small interfering RNA (siRNA) were detected by cell counting kit (CCK)-8, western blot analysis, and Transwell migration and invasion assays. The results revealed that the expression of Prx II in GC tissues and GC cells were significantly upregulated compared with the normal control. There was a significant association between the expression level of Prx II and various factors, including tumor size, histological differentiation, the depth of invasion, the stage of tumor-node-metastasis (TNM) and lymph node metastasis in GC (P<0.05). Survival in patients with higher Prx II expression was significantly decreased compared with those with lower Prx II expression (P<0.01). Prx II, depth of invasion, lymph node metastasis and distant metastasis were identified as independent prognosis factors of GC (P<0.05). Knockdown of Prx II significantly suppressed the proliferation and the migration of GC cells. These experiments revealed that Prx II promotes the development of GC, affecting the survival of patients with GC.
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http://dx.doi.org/10.3892/ol.2018.9208DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6126214PMC
October 2018

Hyperspectral scanning laser optical tomography.

J Biophotonics 2019 04 9;12(4):e201800221. Epub 2018 Dec 9.

Key Laboratory of Advanced Optical Precision Manufacturing Technology of Guangdong Higher Education Institutes, Shenzhen Technology University, Shenzhen, China.

In order to study physical relationships within tissue volumes or even organism-level systems, the spatial distribution of multiple fluorescent markers needs to be resolved efficiently in three dimensions. Here, rather than acquiring discrete spectral images sequentially using multiple emission filters, a hyperspectral scanning laser optical tomography system is developed to obtain hyperspectral volumetric data sets with 2-nm spectral resolution of optically transparent mesoscopic (millimeter-centimeter) specimens. This is achieved by acquiring a series of point-scanning hyperspectral extended depth of field images at different angles and subsequently tomographically reconstructing the 3D intensity distribution for each wavelength. This technique is demonstrated to provide robust measurements via the comparison of spectral and intensity profiles of fluorescent bead phantoms. Due to its enhanced spectral resolving ability, this technique is also demonstrated to resolve largely overlapping fluorophores, as demonstrated by the 3D fluorescence hyperspectral reconstruction of a dual-labeled mouse thymus gland sample and the ability to distinguish tumorous and normal tissues of an unlabeled mouse intestine sample.
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http://dx.doi.org/10.1002/jbio.201800221DOI Listing
April 2019

Analyzing modal power in multi-mode waveguide via machine learning.

Opt Express 2018 Aug;26(17):22100-22109

A machine learning assisted modal power analyzing scheme designed for optical modes in integrated multi-mode waveguides is proposed and studied in this work. Convolutional neural networks (CNNs) are successfully trained to correlate the far-field diffraction intensity patterns of a superposition of multiple waveguide modes with its modal power distribution. In particular, a specialized CNN is trained to analyze thin optical waveguides, which are single-moded along one axis and multi-moded along the other axis. A full-scale CNN is also trained to cross-validate the results obtained from this specialized CNN model. Prediction accuracy for modal power is benchmarked statistically with square error and absolute error distribution. It is found that the overall accuracy of our trained specialized CNN is very satisfactory for thin optical waveguides while that of our trained full-scale CNN remains nearly unchanged but the training time doubles. This approach is further generalized and applied to a waveguide that is multi-moded along both horizontal and vertical axes and the influence of noise on our trained network is studied. Overall, we find that the performance in this general condition keeps nearly unchanged. This new concept of analyzing modal power may open the door for high fidelity information recovery in far field and holds great promise for potential applications in both integrated and fiber-based spatial-division demultiplexing.
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http://dx.doi.org/10.1364/OE.26.022100DOI Listing
August 2018

Structural elucidation of galactomannan from seeds of Crotalaria mucronata Desv. by atomic force microscopy.

Mol Med Rep 2018 Mar 14;17(3):3870-3876. Epub 2017 Dec 14.

School of Food Engineering and Biotechnology, Hanshan Normal University, Chaozhou, Guangdong 521041, P.R. China.

A non-ionic water-soluble galactomannan, isolated from the seed endosperm of Crotalaria mucronata Desv., may be an ideal thickener to increase food viscosity. The present study hypothesized that the viscous property of Crotalaria galactomannan may be associated with its spatial structure. Therefore, the structure of Crotalaria galactomannan was elucidated using an atomic force microscope. The results of the present study demonstrated that the polysaccharide consisted of a D‑mannose backbone with D‑galactose branches, and the D‑mannose/D‑galactose ratio was 2.375:1. In the three‑dimensional structure of Crotalaria galactomannan, the helix was a common secondary structure, containing numerous ring structures of different sizes. In addition, multiple helixes may link together via hydrogen bonding and van der Waals forces, forming aggregations with small rings or spiral windings. The results of the present study indicated that the multiple‑branching construction of Crotalaria galactomannan may underlie its viscosity‑enhancing properties in the water phase.
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http://dx.doi.org/10.3892/mmr.2017.8280DOI Listing
March 2018

Selective adsorption activities toward organic dyes and antibacterial performance of silver-based coordination polymers.

J Colloid Interface Sci 2018 Feb 27;512:730-739. Epub 2017 Oct 27.

Beijing Key Laboratory of Functional Materials for Building Structure and Environment Remediation/Sino-Dutch R&D Centre for Future Wastewater Treatment Technologies, Beijing University of Civil Engineering and Architecture, Beijing 100044, China.

Two silver-based coordination polymers, [Ag(bpy)(cbda)] (BUC-51) and [Ag(bpy)(cpda)]·(NO)·9HO (BUC-52), have been successfully prepared by slow evaporation at room temperature. These coordination polymers exhibited good adsorptive performances toward series organic dyes with sulfonic groups, which could be ascribed to the AgcdotsO interaction between the silver(I) atoms in CPs and the oxygen atoms from sulfonic groups attached to organic dyes. Both BUC-51 and BUC-52 favoured slow release of Ag ions resulting into outstanding long-term antibacterial abilities toward Gram-negative bacteria, Escherichia coli (E. coli), which was tested by a minimal inhibition concentration (MIC) benchmark and an inhibition zone testing method. Both scanning electron microscope (SEM) and transmission electron microscope (TEM) images demonstrated that these two Ag-based coordination polymers could destroy the bacterial membrane and further cause death. Additionally, the excellent stability in common solvents and good optical stability under UV-visible light facilitated their adsorptive and antibacterial applications.
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http://dx.doi.org/10.1016/j.jcis.2017.10.099DOI Listing
February 2018

Over-expression of the Arabidopsis formate dehydrogenase in chloroplasts enhances formaldehyde uptake and metabolism in transgenic tobacco leaves.

Planta 2018 Feb 7;247(2):339-354. Epub 2017 Oct 7.

Faculty of Life Science and Biotechnology, Kunming University of Science and Technology, Chenggong, Kunming, 650500, China.

Main Conclusion: Over-expression of AtFDH controlled by the promoter of Rubisco small subunit in chloroplasts increases formaldehyde uptake and metabolism in tobacco leaves. Our previous study showed that formaldehyde (HCHO) uptake and resistance in tobacco are weaker than in Arabidopsis. Formate dehydrogenase in Arabidopsis (AtFDH) is a key enzyme in HCHO metabolism by oxidation of HCOOH to CO, which enters the Calvin cycle to be assimilated into glucose. HCHO metabolic mechanism in tobacco differs from that in Arabidopsis. In this study, AtFDH was over-expressed in the chloroplasts of transgenic tobacco using a light inducible promoter. C-NMR analysis showed that the carbon flux from HCHO metabolism was not introduced into the Calvin cycle to produce glucose in transgenic tobacco leaves. However, the over-expression of AtFDH significantly enhanced the HCHO metabolism in transgenic leaves. Consequently, the productions of [4-C]Asn, [3-C]Gln, [U-C]oxalate, and HCOOH were notably greater in transgenic leaves than in non-transformed leaves after treatment with HCHO. The increased stomatal conductance and aperture in transgenic leaves might be ascribed to the increased yield of oxalate in the guard cells with over-expressed AtFDH in chloroplasts. Accordingly, the transgenic plants exhibited a stronger capacity to absorb gaseous HCHO. Furthermore, the higher proline content in transgenic leaves compared with non-transformed leaves under HCHO stress might be attributable to the excess formate accumulation and Gln production. Consequently, the HCHO-induced oxidative stress was reduced in transgenic leaves.
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http://dx.doi.org/10.1007/s00425-017-2790-9DOI Listing
February 2018

Safety, pharmacokinetics, and pharmacodynamics of BMS-986142, a novel reversible BTK inhibitor, in healthy participants.

Eur J Clin Pharmacol 2017 Jun 6;73(6):689-698. Epub 2017 Mar 6.

Bristol-Myers Squibb, 3551 Lawrenceville Rd, Princeton, NJ, 08540, USA.

Purpose: BMS-986142 is an oral, small-molecule reversible inhibitor of Bruton's tyrosine kinase. The main objectives of our phase I studies were to characterize the safety and tolerability, pharmacokinetics, and pharmacodynamics of BMS-986142 in healthy participants, and to investigate the potential for the effect of BMS-986142 on the PK of methotrexate (MTX) in combination.

Methods: In a combined single ascending dose and multiple ascending dose study, the safety, pharmacokinetics, and pharmacodynamics of BMS-986142 were assessed in healthy non-Japanese participants following administration of a single dose (5-900 mg) or multiple doses (25-350 mg, once daily for 14 days). In a drug-drug interaction study, the effect of BMS-986142 (350 mg, once daily for 5 days) on the single-dose pharmacokinetics of MTX (7.5 mg) was assessed in healthy participants.

Results: BMS-986142 was generally well tolerated, alone and in combination with MTX. BMS-986142 was rapidly absorbed with peak concentrations occurring within 2 h, and was eliminated with a mean half-life ranging from 7 to 11 h. Exposure of BMS-986142 appeared dose proportional within the dose ranges tested. A dose- and concentration-dependent inhibition of CD69 expression was observed following administration of BMS-986142. BMS-986142 did not affect the pharmacokinetics of MTX.

Conclusions: BMS-986142 was well tolerated at the doses tested, had pharmacokinetic and pharmacodynamic profiles which support once-daily dosing, and can be coadministered with MTX without the pharmacokinetic interaction of BMS-986142 on MTX.
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http://dx.doi.org/10.1007/s00228-017-2226-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5423977PMC
June 2017

In Vitro Metabolite Formation in Human Hepatocytes and Cardiomyocytes and Metabolism and Tissue Distribution in Monkeys of the 2'-C-Methylguanosine Prodrug BMS-986094.

Int J Toxicol 2017 Jan 1:1091581816683642. Epub 2017 Jan 1.

2 Bristol-Myers Squibb Company, Mt Vernon, IN, USA.

BMS-986094, a 2'-C-methylguanosine prodrug for the treatment of chronic hepatitis C virus infection, was withdrawn from phase 2 clinical trials because of unexpected cardiac and renal toxicities. To better understand these toxicities, the in vitro metabolism of BMS-986094 in human hepatocytes (HHs) and human cardiomyocytes (HCMs) and the measurement of BMS-986094 and selected metabolites in monkey plasma and tissues were assessed. BMS-986094 was extensively metabolized by HHs and HCMs, resulting in more efficient formation and accumulation of the active triphosphorylated metabolite, INX-09114, and less efficient efflux of metabolites in HCMs. The predominant metabolism pathway (hydrolysis) in HHs and HCMs was not associated with the formation of reactive metabolites or oxidative stress. In cynomolgus monkeys dosed with BMS-986094 of 15 or 30 mg/kg/d for 3 weeks, the nucleoside metabolite M2 was the major plasma analyte (66%-68% of the combined area under the curve). INX-09114 was the highest drug-related species in the heart and kidney (2,610-4,280 ng/mL [males]; ∼2-420× the concentration of other analytes). Other analytes increased dose dependently, with BMS-986094 highest in diaphragm (≤4,400 ng/mL) followed by M2 in liver and kidney (≤1,360 ng/mL), and M7 and M8 in other tissues (≤124 ng/mL). Three weeks after the last dose, INX-09114 remained high in the heart and kidney (≤1,870 ng/mL), with low M2 (≤37 ng/mL) in plasma and tissues. Persistent high concentrations of INX-09114 in the heart and kidney appeared to correlate with toxicities in these tissues in monkeys.
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http://dx.doi.org/10.1177/1091581816683642DOI Listing
January 2017

Identification of Four Kinds of 2',3'-cNMPs in Escherichia coli and a Method for Their Preparation.

ACS Chem Biol 2016 09 18;11(9):2414-9. Epub 2016 Jul 18.

Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology , Qingdao, China.

Four kinds of 2',3'-cNMPs have been identified in animals and plants, and their physiological roles are also suggested. However, in prokaryotes, while 2',3'-cCMP and 2',3'-cUMP are reported, 2',3'-cGMP or 2',3'-cAMP have never been identified from bacteria or archaea. In addition, there has been no biological method to prepare these cyclic nucleotides. During the study of a novel gene LfliZ from deep-sea bacterium Pseudoalteromonas sp. SM9913, we found that the recombinant LfliZ oligomers contained endogenous substrates, which were then identified to be 2',3'-cCMP, 2',3'-cUMP, 2',3'-cGMP, and 2',3'-cAMP, showing the first evidence for the biological existence of four kinds of 2',3'-cNMPs in prokaryotes. In addition, we further developed a biological method to simultaneously prepare four kinds of 2',3'-cNMPs from Escherichia coli through expressing LfliZ.
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http://dx.doi.org/10.1021/acschembio.6b00426DOI Listing
September 2016