Publications by authors named "Ang Chen"

95 Publications

Transfer of exosomal microRNA-203-3p from dendritic cells to bone marrow-derived macrophages reduces development of atherosclerosis by downregulating Ctss in mice.

Aging (Albany NY) 2021 Jun 2;13. Epub 2021 Jun 2.

Department of Cardiology, Yulin First People’s Hospital and The Sixth Affiliated Hospital of Guangxi Medical University, Yulin 537000, P.R. China.

Dendritic cell-derived exosomes have been proven to be efficient adjuvant options for anti-tumor vaccines in cancer immunotherapy. However, their potency in atherosclerosis remains unclear. Here we summarize the association of microRNA-203-3p (miR-203-3p) with dendritic cell-derived exosomes and atherosclerosis. Firstly, dendritic cell-derived exosomes and bone marrow-derived macrophages were isolated, after which expression of miR-203-3p and cathepsin S was determined. After the establishment of atherosclerosis mouse models, gain- and loss-of-function experiments were conducted for the analysis of effects of miR-203-3p and cathepsin S on foam-cell formation, lipid accumulation, collagen deposition and serum total cholesterol. The results found high expression of cathepsin S in atherosclerosis mice and downregulation of miR-203-3p in the serum of atherosclerosis patients and ox-LDL-simulated bone marrow-derived macrophages. Cathepsin S was the target gene of miR-203-3p. miR-203-3p transporting from exosomes to bone marrow-derived macrophages resulted in inhibition of cathepsin S expression and atherosclerosis-related phenotypes in bone marrow-derived macrophages, thus alleviating atherosclerosis in mice, and this process was found to involve the p38/MAPK signaling pathway. These findings provided evidence that the transfer of miR-203-3p by dendritic cell-derived exosomes targeted cathepsin S in bone marrow-derived macrophages to attenuate atherosclerosis progression in mice, serving as a promising clinical target for atherosclerosis.
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http://dx.doi.org/10.18632/aging.103842DOI Listing
June 2021

Residue changes and processing factors of eighteen field-applied pesticides during the production of Chinese Baijiu from rice.

Food Chem 2021 Oct 29;359:129983. Epub 2021 Apr 29.

Longping Branch, Graduate School of Hunan University, Changsha 410125, China; Hunan Plant Protection Institute, Hunan Academy of Agricultural Science, Changsha 410125, China. Electronic address:

The fate of eighteen pesticides in field-collected rice samples during Chinese Baijiu production was systematically studied. The results indicated that steeping decreased flonicamid residue by 73.2% due to its high water-solubility and low octanol/water partition coefficient. The steaming step reduced pesticide residues by 32.0%-75.3% through evaporation or thermal degradation. After steaming, the pesticide residues were further reduced by 39.8-74.2% in fermentation which might be caused by biological degradation. In addition, distillation was shown to be most effective, responsible for greater than 90% losses of the remaining pesticide residues. The processing factors (PFs) were generally lower than 1 for different processes and the whole procedure. These results revealed that the procedure of Chinese Baijiu production could dramatically decrease residues of all the eighteen pesticides. Overall, this study provide important references for monitoring pesticide residue levels during the production of Chinese Baijiu from rice, and ensuring proper risk assessment from pesticide contamination.
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http://dx.doi.org/10.1016/j.foodchem.2021.129983DOI Listing
October 2021

Residue behavior, transfer and risk assessment of tolfenpyrad, dinotefuran and its metabolites during tea growing and tea brewing.

J Sci Food Agric 2021 Apr 13. Epub 2021 Apr 13.

Longping Branch, Graduate School of Hunan University, Changsha, China.

Background: Tolfenpyrad and dinotefuran are two representative pesticides used for pest control in tea gardens. Their application may bring about a potential risk to the health of consumers. Therefore, it is essential to investigate the residue behavior, transfer and risk assessment of tolfenpyrad, dinotefuran and metabolites from tea garden to teacup.

Results: An effective analytical method was established and validated to simultaneously determine tolfenpyrad, dinotefuran and its metabolites (DN and UF) in tea. The average recoveries of tolfenpyrad, dinotefuran, DN and UF were in the range 72.1-106.3%, with relative standard deviations lower than 11.8%. On the basis of the proposed method, the dissipation of tolfenpyrad and dinotefuran in fresh tea leaves followed first-order kinetics models with half-lives of 4.30-7.33 days and 4.65-5.50 days, respectively. With application amounts of 112.5-168.75 g a.i. ha once or twice, the terminal residues of tolfenpyrad and total dinotefuran in green tea were lower than 19.6 and 7.13 mg kg , respectively, and below their corresponding maximum residue limits . The leaching rates of tolfenpyrad and total dinotefuran during the tea brewing were in the ranges 1.4-2.3% and 93.7-98.1%, respectively.

Conclusion: Tolfenpyrad and dinotefuran in tea were easily degraded. The RQ and RQ values for tolfenpyrad were 37.6% and 5.4%, which were much higher than for dinotefuran at 24.7% and 0.84%, respectively. The data indicated that there was no significant health risk in tea for consumers at the recommended dosages. The results provide scientific data regarding the reasonable use of tolfenpyrad and dinotefuran aiming to ensure safe tea consuption. © 2021 Society of Chemical Industry.
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http://dx.doi.org/10.1002/jsfa.11253DOI Listing
April 2021

Learners' motivational response to the Science, PE, & Me! curriculum: A situational interest perspective.

J Sport Health Sci 2021 03 6;10(2):243-251. Epub 2019 Nov 6.

Department of Kinesiology, University of North Carolina at Greensboro, Greensboro, NC 27402, USA.

Background: The Science, PE, & Me! (SPEM) curriculum is a concept-based physical education curriculum that offers students coherent educational experiences for constructing health-related fitness knowledge through movement experiences. The purpose of this study was to evaluate students' motivational response to the SPEM curriculum from the situational interest perspective.

Methods: The study used a cluster randomized controlled design in which 30 elementary schools in one of the largest metropolitan areas in the eastern United States were randomly assigned to an experimental or comparison condition. Although all students in the 3rd, 4th, and 5th grades in the targeted schools were eligible to participate in the study, a random sample of students from the experimental (n = 1749; 15 schools) and comparison groups (n = 1985; 15 schools) provided data. Students' motivational response to the SPEM curriculum or comparison curriculum was measured using the previously validated Situational Interest Scale-Elementary. Data were analyzed using structural mean modeling.

Results: The results demonstrated that the experimental group (as reference group) showed significantly higher enjoyment (z = -2.01), challenge (z = -6.54), exploration (z = -12.195), novelty (z = -8.80), and attention demand (z = -7.90) than the comparison group.

Conclusion: The findings indicate that the SPEM curriculum created a more situationally interesting context for learning than the comparison physical education curriculum.
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http://dx.doi.org/10.1016/j.jshs.2019.11.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7987648PMC
March 2021

Three-wavelength optical sensor for measuring distributed mass concentrations of aerosols from stationary sources.

Opt Express 2021 Mar;29(5):6407-6423

Stationary source emissions of key industries, such as thermal power plants, have become the central consideration in environmental protection programs. Existing photoelectric sensors at stationary sources usually use a single wavelength laser to measure the total mass concentration of the particulate matter, bearing inherent errors due to the changing particle size distribution (PSD). However, the total mass concentration cannot comprehensively estimate the air pollution caused by the stationary sources. Therefore, it is required to measure both the mass concentration and PSD of the aerosols emitted by the stationary sources, based on which we can get a distributed mass concentration. To implement this, in this study, we designed a novel three-wavelength photoelectric sensor and tested its performance. Results showed that the prototype correctly determines the mean particle size and standard deviation of the PSDs and consequently adjusts the coefficient for measuring the mass concentration from light intensity, providing a comprehensive assessment of the pollutants.
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http://dx.doi.org/10.1364/OE.415490DOI Listing
March 2021

Phase characterisation of metalenses.

Light Sci Appl 2021 Mar 10;10(1):52. Epub 2021 Mar 10.

Department of Electronic and Information Engineering, The Hong Kong Polytechnic University, 999077, Hong Kong, China.

Metalenses have emerged as a new optical element or system in recent years, showing superior performance and abundant applications. However, the phase distribution of a metalens has not been measured directly up to now, hindering further quantitative evaluation of its performance. We have developed an interferometric imaging phase measurement system to measure the phase distribution of a metalens by taking only one photo of the interference pattern. Based on the measured phase distribution, we analyse the negative chromatic aberration effect of monochromatic metalenses and propose a feature size of metalenses. Different sensitivities of the phase response to wavelength between the Pancharatnam-Berry phase-based metalens and propagation phase-reliant metalens are directly observed in the experiment. Furthermore, through phase distribution analysis, it is found that the distance between the measured metalens and the brightest spot of focusing will deviate from the focal length when the metalens has a low nominal numerical aperture, even though the metalens is ideal without any fabrication error. We also use the measured phase distribution to quantitatively characterise the imaging performance of the metalens. Our phase measurement system will help not only designers optimise the designs of metalenses but also fabricants distinguish defects to improve the fabrication process, which will pave the way for metalenses in industrial applications.
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http://dx.doi.org/10.1038/s41377-021-00492-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947014PMC
March 2021

Investigation of the content differences of arachidonic acid metabolites in a mouse model of breast cancer by using LC-MS/MS.

J Pharm Biomed Anal 2021 Feb 20;194:113763. Epub 2020 Nov 20.

Changning Maternity and Infant Health Hospital, East China Normal University, Shanghai, China; Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, China. Electronic address:

Arachidonic acid (AA) is closely associated with breast cancer. In addition to the two metabolic pathways regulated by cyclooxygenase and lipoxygenase, AA has a third metabolic pathway through which cytochrome P450 (CYP) enzymes produce hydroxyeicosatetraenoic acids (HETEs) and epoxyeicosatrienoic acids (EETs). The targeted CYP-mediated pathway of AA can not only kill cancer cells but also inhibit the interstitial microenvironment around a tumor. Therefore, it makes sense to identify potential biomarkers from the AA metabolome for the diagnosis and treatment of breast cancer. This study established a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the analysis of AA and its main metabolites, EETs and HETEs, in MMTV-PyMT mice, a spontaneous breast cancer mouse model. The results showed that there were significant differences in the concentrations of AA, 12-HETE, 19-HETE and 8,9-EET in plasma and tumor tissues between normal and MMTV-PyMT mice. Therefore, the eicosanoids mentioned above may be used as new biomarkers for breast cancer diagnosis. This study provides a new perspective for the recognition and diagnosis of breast cancer.
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http://dx.doi.org/10.1016/j.jpba.2020.113763DOI Listing
February 2021

Structural characterization and functional activity of an exopolysaccharide secreted by Rhodopseudomonas palustris GJ-22.

Int J Biol Macromol 2021 Jan 27;167:160-168. Epub 2020 Nov 27.

Hunan Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China. Electronic address:

One water exopolysaccharide, designated G-EPS, was secreted by Rhodopseudomonas palustris GJ-22 culture media. The structure of G-EPS was characterized with HPGPC, GC-MS, methylation, 1D and 2D NMR, along with UV and FT-IR spectrum. The G-EPS molecular weight was 10.026 kilodalton, and is composed of D-mannose (92.8%) and d-glucose (7.2%). The purified G-EPS promoted plant growth and induced systemic resistance against TMV in Nicotiana benthamiana. These results suggested that G-EPS is an important active component of the bio-control capacity of GJ-22.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.11.165DOI Listing
January 2021

Machine-learning enabled wireless wearable sensors to study individuality of respiratory behaviors.

Biosens Bioelectron 2020 Nov 6;173:112799. Epub 2020 Nov 6.

School of Electrical, Computer, and Energy Engineering, Arizona State University, Tempe, AZ, 85281, USA.

Respiratory behaviors provide useful measures of lung health. The current methods have limited capabilities of continuous characterization of respiratory behaviors, often required to assess respiratory disorders and diseases. This work presents a system equipped with a machine learning algorithm, capable of continuously monitoring respiratory behaviors. The system, consisting of two wireless wearable sensors, accurately extracts and classifies the features of respiratory behaviors of subjects within various postures, wirelessly transmitting the temporal respiratory behaviors to a laptop. The sensors were attached on the midway of the xiphoid process and the costal margin, and 1 cm above the umbilicus, respectively. The wireless wearable sensor, consisting of ultrasound emitter, ultrasound receiver, data acquisition and wireless transmitter, has a small footprint and light weight. The sensors correlate the mechanical strain at wearing sites to lung volume by measuring the local circumference changes of the chest and abdominal walls simultaneously. Eleven subjects were recruited to evaluate the wireless wearable sensors. Three different random forest classifiers, including generic, individual, and weighted-adaptive classifiers, were used to process the wireless data of the subjects at four different postures. The results demonstrate the respiratory behaviors are individual- and posture-dependent. The generic classifier merely reaches the accuracy of classifying postures of 21.9 ± 1.7% while individual and weighted-adaptive classifiers mark substantially high, up to 98.9 ± 0.6% and 98.8 ± 0.6%, respectively. The accurate monitoring of respiratory behaviors can track the progression of respiratory disorders and diseases, including chronic respiratory obstructive disease (COPD), asthma, apnea, and others for timely and objective approaches for control.
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http://dx.doi.org/10.1016/j.bios.2020.112799DOI Listing
November 2020

Activation of activin/Smad2 and 3 signaling pathway and the potential involvement of endothelial‑mesenchymal transition in the valvular damage due to rheumatic heart disease.

Mol Med Rep 2021 01 12;23(1). Epub 2020 Nov 12.

Department of Cardiology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.

Rheumatic heart disease (RHD) is an autoimmune disease caused by rheumatic fever following group A hemolytic streptococcal infection and primarily affects the mitral valve. RHD is currently a major global health problem. However, the exact pathological mechanisms associated with RHD‑induced cardiac valve damage remain to be elucidated. The endothelial‑mesenchymal transition (EndMT) serves a key role in a number of diseases with an important role in cardiac fibrosis and the activin/Smad2 and 3 signaling pathway is involved in regulating the EndMT. Nevertheless, there are no studies to date, to the best of the authors' knowledge, investigating the association between RHD and EndMT. Thus, the aim of the current study was to investigate the potential role of EndMT in cardiac valve damage and assess whether activin/Smad2 and 3 signaling was activated during RHD‑induced valvular injury in a rat model of RHD induced by inactivated Group A streptococci and complete Freund's adjuvant. Inflammation and fibrosis were assessed by hematoxylin and eosin and Sirius red staining. Serum cytokine and rheumatoid factor levels were measured using ELISA kits. Expression levels of activin/Smad2 and 3 signaling pathway‑related factors [activin A, Smad2, Smad3, phosphorylated (p‑)Smad2 and p‑Smad3], EndMT‑related factors [lymphoid enhancer factor‑1 (LEF‑1), Snail1, TWIST, zinc finger E‑box‑binding homeobox (ZEB)1, ZEB2, α smooth muscle actin (α‑SMA) and type I collagen α 1 (COL1A1)], apoptosis‑related markers (BAX and cleaved caspase‑3) and valvular inflammation markers (NF‑κB and p‑NF‑κB) were detected using reverse transcription‑quantitative PCR and western blot analyses. Compared with the control group, the degree of valvular inflammation and fibrosis, serum levels of IL‑6, IL‑17, TNF‑α and expression of apoptosis‑related markers (BAX and cleaved caspase‑3) and valvular inflammation marker (p‑NF‑κB), activin/Smad2 and 3 signaling pathway‑related factors (activin A, p‑Smad2 and p‑Smad3), EndMT‑related factors (LEF‑1, Snail1, TWIST, ZEB 1, ZEB2, α‑SMA and COL1A1) were significantly increased in the RHD group. These results suggested that the activin/Smad2 and 3 signaling pathway was activated during the development of valvular damage caused by RHD and that the EndMT is involved in RHD‑induced cardiac valve damage.
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http://dx.doi.org/10.3892/mmr.2020.11648DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7673319PMC
January 2021

Detecting urine metabolites of bladder cancer by surface-enhanced Raman spectroscopy.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Feb 24;247:119108. Epub 2020 Oct 24.

Department of Urology, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, 44 Xiaoheyan Road, Shenyang, Liaoning 110042, China. Electronic address:

Aim: Metabolites present in urine reflect the current phenotype of the cancer state. Surface-enhanced Raman spectroscopy (SERS) can be used in urine supernatant or sediment to largely reflect the metabolic status of the body.

Materials & Methods: SERS was performed to detect bladder cancer (BCa) and predict tumour grade from urine supernatant, which contains various system metabolites, as well as from urine sediment, which contains exfoliated tumour cells.

Results & Discussion: Upon combining the urinary supernatant and sediment results, the total diagnostic sensitivity and specificity of SERS were 100% and 98.85%, respectively, for high-grade tumours and 97.53% and 90.80%, respectively, for low-grade tumours.

Conclusion: The present results suggest high potential for SERS to detect BCa from urine, especially when combining both urinary supernatant and sediment results.
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http://dx.doi.org/10.1016/j.saa.2020.119108DOI Listing
February 2021

Study on degradation behaviour, residue distribution, and dietary risk assessment of propiconazole in celery and onion under field application.

J Sci Food Agric 2021 Mar 5;101(5):1998-2005. Epub 2020 Oct 5.

Longping Branch, Graduate School of Hunan University, Changsha, 410125, China.

Background: Propiconazole is widely used to control fungal diseases in field crops, including celery and onion. The potential risk to the environment and human health has aroused much public concern. Therefore, it is significant to investigate the degradation behaviour, residue distribution, and dietary risk assessment of propiconazole in celery and onion.

Results: A sensitive analytical method for determination of propiconazole residue in celery and onion was established and validated through high-performance liquid chromatography tandem mass spectrometry. The average recovery rate of propiconazole ranged from 85.7% to 101.8%, with a relative standard deviation of 2.1-6.3%. For the dissipation kinetics, the data showed that propiconazole in celery and onion was degraded, with half-lives of 6.1-6.2 days and 8.7-8.8 days respectively. In the terminal residue experiments, the residues of propiconazole were below 4.66 mg kg in celery after application two or three times and were below 0.029 mg kg in onion after application of three or four times with an interval of 14 days under the designed dosages. The chronic and acute dietary exposure assessments for propiconazole were valued by risk quotient, with all values being lower than 100%.

Conclusion: Propiconazole in celery and onion was rapidly degraded following first-order kinetics models. The dietary risk of propiconazole through celery or onion was negligible to consumers. The study not only offers a valuable reference for reasonable usage of propiconazole on celery and onion, but also facilitates the establishment of maximum residue limits in China. © 2020 Society of Chemical Industry.
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http://dx.doi.org/10.1002/jsfa.10817DOI Listing
March 2021

Ultrawideband, Wide Scanning Stripline-Fed Tightly Coupled Array Antenna Based on Parallel-Dipole Elements.

Sensors (Basel) 2020 Sep 6;20(18). Epub 2020 Sep 6.

Department of Physics, Fudan University, Shanghai 200438, China.

A stripline-fed tightly coupled array antenna with compact size, large scan volume and low cross-polarization characteristics is proposed for ultrawideband (UWB) applications. Simple impedance-matching process is realized by using parallel dual dipoles. Meanwhile, the parallel symmetrical radiating structures minimize the cross-polarization field components dramatically. The mitigation of various undesired resonances is studied in detail. An infinite array is designed to achieve 3:1 bandwidth (6-18 GHz) when scanning ±60∘ in the E-/D-planes (VSWR < 2.5) and H-plane (VSWR < 3.5). The cross-polarization levels remain below -29 dB at broadside. A 16 × 16 prototype is fabricated to demonstrate the design. The measured results are consistent well with the simulated ones. The overall size of the prototype at the lowest operating frequency is 3×3×0.4λ03 (15×15×2cm3). Due to its wide bandwidth, good electronic scan performance and compact size, the proposed antenna array is a good candidate for modern wireless platforms.
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http://dx.doi.org/10.3390/s20185065DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7571110PMC
September 2020

Generation and Characterization of Cytochrome P450 2J3/10 CRISPR/Cas9 Knockout Rat Model.

Drug Metab Dispos 2020 11 1;48(11):1129-1136. Epub 2020 Sep 1.

Changning Maternity and Infant Health Hospital (J.L., Y.Z., Y.G., X.W.), and Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences (J.L., A.C., X.M., X.S., Y.Z., M.L., X.W.), East China Normal University, Shanghai, People's Republic of China

Cytochrome P450 2J2 (CYP2J2) enzyme attracts more attention because it not only metabolizes clinical drugs but also mediates the biotransformation of important endogenous substances and the regulation of physiologic function. Although CYP2J2 is very important, few animal models are available to study its function In particular, a gene knockout (KO) rat model for drug metabolism and pharmacokinetics is not available. In this report, the CRISPR/Cas9 technology was used to delete rat , the orthologous genes of in humans. The KO rats were viable and fertile and showed no off-target effect. Compared with wild-type (WT) rats, the mRNA and protein expression of in liver, small intestine, and heart of KO rats were completely absent. At the same time, mRNA expression and protein expression were significantly decreased in these tissues. Further and metabolic studies of astemizole, a typical substrate of CYP2J, indicated that CYP2J was functionally inactive in KO rats. The heart function indexes of WT and KO rats were also measured and compared. The myocardial enzymes, including creatine kinase-muscle brain type (CK-MB), creatine kinase (CK), and CK-MB/CK ratio, of KO rats increased by nearly 140%, 80%, and 60%, respectively. In conclusion, this study successfully developed a new KO rat model, which is a useful tool to study the function of CYP2J in drug metabolism and cardiovascular disease. SIGNIFICANCE STATEMENT: Human CYP2J2 is involved not only in clinical drug metabolism but also in the biotransformation of important endogenous substances. Therefore, it is very important to construct new animal models to study its function . This study successfully developed a new CYP2J knockout rat model by using CRISPR/Cas9 technology. This rat model provides a useful tool to study the role of CYP2J in drug metabolism and diseases.
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http://dx.doi.org/10.1124/dmd.120.000114DOI Listing
November 2020

Effects of a Concept-Based Physical Education on Middle School Students' Knowledge, Motivation, and Out-of-School Physical Activity.

J Teach Phys Educ 2020 Jul;39(3):407-414

University of North Carolina-Greensboro, Greensboro, NC.

Purpose: This study aimed to determine the extent to which a concept-based physical education curriculum, specifically the Science of Healthful Living (SHL) curriculum, influenced middle school students' knowledge, motivation for physical education (PE) and physical activity (PA), and out-of-school PA.

Methods: A static group comparison design was adopted to analyze the differences on fitness knowledge, autonomous motivation for PE and PA, and out-of-school PA between eighth-grade students who studied the SHL curriculum (the experimental condition, = 168) and their peers who studied a multiactivity PE (the control condition, = 226) 1 year earlier.

Results: The students who studied the SHL curriculum demonstrated significantly higher levels of knowledge ( < .05, Cohen = 0.81), autonomous motivation toward PA ( < .05, Cohen = 0.20), and out-of-school PA ( < .05, Mann-Whitney effect size = 0.01) than students who had experienced the multiactivity PE. The students in both conditions were equally motivated in their respective PE courses.

Conclusion: The SHL curriculum is effective in promoting students' PA behavior outside of the school.
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http://dx.doi.org/10.1123/jtpe.2019-0067DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7447199PMC
July 2020

Learning to Teach Physical Education for Health: Breaking the Curriculum Safety Zone.

Res Q Exerc Sport 2020 Aug 20:1-14. Epub 2020 Aug 20.

University of North Carolina at Greensboro.

: Based on the theorized concept "Curriculum Safety Zone (CSZ)," this study was conducted to identify the factors for breaking CSZ by contrasting the experiential accounts of two pre-service teacher groups who taught within or outside of their CSZ. : Pre-service teachers ( = 14) from a sport-centered PETE program were trained to teach a Health-First curriculum module and their peers ( = 14) a traditional sport module. Each group taught their respective module to 14 intact 7th grade classes in 14 schools in China. A mixed-methods design was used. Quantitative data on learner knowledge gain were collected from the learners. Qualitative data included lesson observation fieldnotes, social-media posts, and interview responses and were gathered from the pre-service teachers. : Learners in the Health-First schools gained more knowledge than those in the Comparison schools (t = 2.92, = .007, Cohen's d = 1.10). Qualitative evidence was triangulated using a Health-First vs. Comparison contrasting approach with open-, axial-, and selective-coding to generate themes. The themes were Confidence in Doubt, Lesson Plans to the Rescue, Professional Development is Necessary But…, and Student Learning Save the Day! A grounded theory was developed using the themes and interpreted using the Interconnection Model of Teacher Professional Growth. : Breaking CSZ requires a synergistic effort with carefully designed professional development, detailed lesson plans, an effective support network, and, most important of all, a powerful curriculum that can elicit observable and measurable learner achievement.
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http://dx.doi.org/10.1080/02701367.2020.1769008DOI Listing
August 2020

Power of the Curriculum: Content, Context, and Learning in Physical Education.

Res Q Exerc Sport 2020 Aug 18:1-12. Epub 2020 Aug 18.

University of North Carolina Greensboro.

: It is argued that the constructivist physical education has the potential to overcome socioeconomic (SES) constraints and promote learning. The purpose of this study was to determine the extent to which school SES-related class environmental factors influenced student learning in a constructivist physical education context. : Students in 8 grade ( = 1,256) from 10 middle schools with varied SES were tested on exercise knowledge before and after a constructivist physical education intervention. School SES was determined using free and reduced meal ratio (FARM) and physical education related factors. Data on lesson frequency, length, facilities, equipment, and class size were collected from teachers. : Hierarchical linear model analyses reveal that SES is not predictive of intervention induced learning ( = 0.73, =.91, =.37). Lesson frequency ( =.52, =.31, =.06), length ( = -.03, = -1.82, =.07), facilities ( = -.11, = -.49, =.63), equipment ( = -.36, = -1.36, =.18), and class size ( = -.05, = -1.36, =.18) are not predictive of learning. Student prior knowledge ( = -.59, t = -18.37, <.001) and teacher factor ( =.04, = 3.72, <.001) are predictive of learning. : The findings suggest that school SES's detrimental impact on learning in physical education could be overcome by the power of a constructivist curriculum.
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http://dx.doi.org/10.1080/02701367.2020.1768202DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7889760PMC
August 2020

Light Scattering Intensity Field Imaging Sensor for In Situ Aerosol Analysis.

ACS Sens 2020 07 14;5(7):2061-2066. Epub 2020 Jul 14.

School of Electronic Information and Communications, Huazhong University of Science and Technology, Wuhan 430074, China.

Aerosol plays an important role in a broad range of scientific disciplines, such as atmospheric chemistry and physics, fuel combustion, and human health. Current particle sizing instruments are usually bulky, complicated, and expensive, while the portable ones cannot provide sufficient measurement channels to describe the particle size distribution accurately. To address this challenge, we propose an optical sensing method to analyze the particle size distribution of aerosols based on the light scattering intensity field (LSIF). The LSIF is a set of scattering lights in all directions around the particles, which contains the scattering light signals in different observing angles. Then, the particle size distribution of the aerosol samples is retrieved by the Tikhonov regularization algorithm. A portable and low-cost aerosol sizing prototype sensor is designed to image part of the LSIF signals, where the LSIF is collected by a parabolic reflector and projected on the image sensor as an image with telecentric lenses. According to the experimental result of di-ethyl-hexyl-sebacate aerosol test, the relative measurement error of LSIF can be controlled to ±10%. With an integrated and cost-effective design, this particle sizing sensor shows great potential for routine field measurements outside of the laboratory.
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http://dx.doi.org/10.1021/acssensors.0c00574DOI Listing
July 2020

Oridonin Attenuates Myocardial Ischemia/Reperfusion Injury via Downregulating Oxidative Stress and NLRP3 Inflammasome Pathway in Mice.

Evid Based Complement Alternat Med 2020 30;2020:7395187. Epub 2020 May 30.

Guangxi Key Laboratory of Precision Medicine in Cardio-Cerebrovascular Diseases Control and Prevention, Guangxi Clinical Research Center for Cardio-Cerebrovascular Diseases, Nanning, Guangxi 530021, China.

Oridonin (ORI), the major pharmacological component extracted from a traditional Chinese medicine, possesses a beneficial effect on myocardial ischemia/reperfusion (I/R) injury. However, the underlying molecular mechanism by which ORI effects take place is not completely known. Thus, whether ORI works via downregulating oxidative stress and nod-like receptor protein-3 (NLRP3) inflammasome pathway was investigated in this study. Mice underwent surgery to induce myocardial I/R injury, and some were administered ORI (10 mg/kg/day) pretreatment, while others were not. The myocardial enzymes' levels, infarct area, and inflammatory injury were measured. The activation situation of oxidative stress and NLRP3 inflammasome was also detected. We found that ORI pretreatment significantly alleviated CK-MB and cTnI levels and infarct size induced by I/R. ORI mitigated the inflammatory injury by decreasing the pathological damage and lowering TNF-, IL-1, and IL-18 levels. Moreover, the SOD1 and eNOS levels were significantly increased by ORI, while MDA and iNOS levels were relatively decreased. The oxidative stress was reversed using ORI pretreatment. Importantly, NLRP3 inflammasome pathway was also inhibited by ORI, as reflected by the lower protein levels of NLRP3, caspase-1, and IL-1. In conclusion, ORI alleviates myocardial injury induced by I/R via inhibiting the oxidative stress and NLRP3 inflammasome pathway.
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http://dx.doi.org/10.1155/2020/7395187DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7277023PMC
May 2020

circFMN2 Sponges miR-1238 to Promote the Expression of LIM-Homeobox Gene 2 in Prostate Cancer Cells.

Mol Ther Nucleic Acids 2020 Sep 25;21:133-146. Epub 2020 May 25.

Department of Urology, The First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning, China. Electronic address:

Circular RNAs (circRNAs) regulate gene expression in different malignancies. However, the molecular mechanisms that link circRNAs with the tumorigenesis of prostate cancer (PCa) are not well understood. In the present study, we attempted to provide a novel basis for targeted therapy for PCa from the aspect of circRNA-microRNA (miRNA)-mRNA interaction. We investigated the expression of circRNAs in 5 paired PCa tissues and adjacent non-tumor tissues by microarray analysis. We focused on hsa_circ_0005100, which is located on chromosome 1 and derived from FMN2, and thus we named it circFMN2. The qRT-PCR was used to detect circFMN2 and target miRNA expression in PCa tissues and cell lines. Biological functional experiments were performed to detect the effects of circFMN2 on the biological behavior of PCa cells in vivo and in vitro. Bioinformatic analysis was utilized to predict potential miRNA target sites on circFMN2. High expression of circFMN2 was associated with PCa progression. Function assays revealed that knockdown of circFMN2 significantly reduced PCa cell growth in vitro and in vivo. Finally, we found that circFMN2 acts as a competing endogenous RNA (ceRNA) for miR-1238 to regulate LIM-homeobox gene 2 (LHX2) expression. circFMN2 regulates the miR-1238/LHX2 axis to promote PCa progression.
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http://dx.doi.org/10.1016/j.omtn.2020.05.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7286986PMC
September 2020

PCDH7 Inhibits the Formation of Homotypic Cell-in-Cell Structure.

Front Cell Dev Biol 2020 8;8:329. Epub 2020 May 8.

Laboratory of Cell Engineering, Institute of Biotechnology, Beijing, China.

Though homotypic cell-in-cell (hoCIC) structures are implicated in the development and progression of multiple human tumors, the molecular mechanisms underlying their formation remain poorly understood. We found that the expression of Protocadherin-7 (PCDH7), an integral membrane protein, was negatively associated with the formation of hoCIC structures. Overexpression of efficiently inhibits, while its depletion significantly enhances, hoCIC formation, which was attributed to its regulation on intercellular adhesion and contractile actomyosin as well. Via directly interacting with and inactivating PP1α, a protein phosphatase that dephosphorylates pMLC2, PCDH7 increases the level of pMLC2 leading to enhanced actomyosin at the intercellular region and compromised hoCIC formation. Remarkably, enhanced anchorage-independent cell growth in a hoCIC-dependent manner. Together, we identified PCDH7 as the first membrane protein that inhibits hoCIC formation to promote tumor growth.
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http://dx.doi.org/10.3389/fcell.2020.00329DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7225324PMC
May 2020

[The value of P16/ki67 double labeling, HPV E6/E7 mRNA detection and their combined application in cytological shunt diagnosis of low-grade squamous intraepithelial lesions].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2020 Jan;36(1):63-68

Department of Pathology, Cancer Center, Sun Yat-sen University, Guangzhou 510060, China.

Objective To explore the value of double labeling of P16/ki67, E6/E7 mRNA of human papillomavirus (HPV) and combined detection in shunt diagnosis of low-grade squamous intraepithelial lesions (LSIL) by thin-layer cervical cytology (TCT). Methods The study enrolled 239 patients who underwent colposcopy and biopsy within 4 weeks after primary TCT diagnosis. The remaining cytological samples were double-labeled with P16/ki67 immunocytochemical staining and the HPV E6/E7 mRNA was detected by Panther automatic HPV E6/E7 mRNA detection system. Using SPSS22.0 software, the positive rates of P16/ki67 double-labeling, HPV E6/E7 mRNA and combined detection were analyzed in different cervical lesions, and the positive rates in the same cervical lesions were compared horizontally to evaluate the efficiency of double labeling of P16/ki67, HPV E6/E7 and combined detection in the diagnosis of high-grade squamous intraepithelial lesions (HSIL) and above lesions. Results The diagnostic results of HE staining for the 239 cases of LSIL were 71 cases of chronic cervicitis (29.71%), 143 cases of LSIL (59.83%), 22 cases of HSIL (9.20%) and 3 cases of cervical cancer (1.26%). There were 46 cases of P16ki67 lesions (19.25%), 41 cases of ki67P16 lesions (17.15%), 33 cases of ki67P16 lesions (13.81%) and 119 cases of P16-ki67- lesions (49.79%). The positive rates of P16/ki67 double-labeling, HPV E6/E7 mRNA and combined detection increased with the severity of cervical lesions. The positive rate of combined detection was the highest in the HSIL lesions, which was higher than that of P16/ki67 double-labeling and HPV E6/E7 mRNA detection. The sensitivity of combined detection was higher than that of P16/ki67 double-labeling and HPV E6/E7 mRNA detection. The Youden index of joint detection was 0.7850. Conclusion The combined detection of P16/ki67 double labeling, HPV E6/E7 mRNA and HPV E6/E7 mRNA had a certain clinical value in the management of cell LSIL shunt diagnosis. The combined detection significantly improved the sensitivity and Youden index of HSIL and above lesions, while maintaining a high specificity and coincidence rate.
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January 2020

Intrinsic Cerebro-Cerebellar Functional Connectivity Reveals the Function of Cerebellum VI in Reading-Related Skills.

Front Psychol 2020 20;11:420. Epub 2020 Mar 20.

State Key Laboratory of Cognitive Neuroscience and Learning and IDG/McGovern Institute for Brain Research, Beijing Normal University, Beijing, China.

The engagement of the cerebellum VI in reading was reported in both typically developing and dyslexic readers. However, it is still not clear how the cerebellum VI contributes to reading. Here we have examined the correlation of intrinsic cerebro-cerebellar functional connectivity with two critical reading-related skills-phonological awareness (PA) and rapid automatized naming (RAN)-with fMRI technology. Specifically, we tested the hypothesis that the cerebellum may contribute to reading either by phonological skills or by automatizing skills. We chose the left and right cerebellum VI as ROIs, and we calculated the intrinsic cerebro-cerebellar functional connectivity during a resting state. We further explored whether and how cerebro-cerebellar resting state functional connectivity (RSFC) is associated with individuals' reading-related skills including PA and RAN. The results showed that the functional connectivity between the left supramarginal gyrus and bilateral cerebellum VI was related to RAN, and the connectivity between the left insula and right cerebellum VI was related to PA. However, the effect of PA did not survive after the RAN was regressed out. Control analyses further confirmed that it was the intrinsic cerebro-cerebellar functional connectivity rather than the local cerebellar functionality that associated with phonological awareness ability and rapid automatized naming ability. For the first time, the relationship between cerebro-cerebellar resting state functional connectivity and specific reading-related skills has been explored, and this has deepened our understanding of the way the cerebellum VI is involved in reading.
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http://dx.doi.org/10.3389/fpsyg.2020.00420DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7099000PMC
March 2020

Cross-polarization suppression for patch array antennas via generalized Kerker effects.

Opt Express 2020 Jan;28(1):40-47

The generalized Kerker effect has recently gained an explosive progress in metamaterials, from the scattering management of particle clusters to the reflection and transmission manipulation of metalattices and metasurfaces. Various optical phenomena observed can be explained by the generalized Kerker effect. Due to the same nature of electromagnetic waves, we believe that the generalized Kerker effect can also be used in the microwave field. Inspired by this, in this letter we design a kind of patch array antenna to suppress the cross-polarization by interferences of multipoles. Using different far-field radiation phase symmetries of electromagnetic multipoles for the patch, the cross-polarization can be almost cancelled while the co-polarization be kept. A pair of 8×8 U-slot patch array antennas, working in a wide band (8.8 GHz-10.4 GHz), have been designed, fabricated and measured to verify our proposal. Simulated and measured results both agree well with the theory, showing more than 20 dB gain suppression of the cross-polarization, which indicates the universality of the generalized Kerker effect in electromagnetic waves.
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http://dx.doi.org/10.1364/OE.28.000040DOI Listing
January 2020

A clash of fundamental assumptions: Can/should we measure physical literacy?

Authors:
Ang Chen

J Sport Health Sci 2020 03 21;9(2):149-151. Epub 2019 Nov 21.

Department of Kinesiology, University of North Carolina at Greensboro, Greensboro, NC 27412, USA. Electronic address:

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http://dx.doi.org/10.1016/j.jshs.2019.11.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7031782PMC
March 2020

Parental preferences in treatment of acute uncomplicated appendicitis comparing surgery to conservative management with antibiotics and their views on research participation.

Eur J Pediatr 2020 May 3;179(5):735-742. Epub 2020 Jan 3.

Department of Paediatric Surgery, KK Women's and Children's Hospital, 100 Bukit Timah Road, Singapore, 229899, Singapore.

Studies show that conservative management in acute uncomplicated appendicitis (AUA) is an alternative to surgery. This study aims to determine factors affecting parental preference in management of AUA and their decision for research participation. We conducted surveys on parents whose children were admitted with suspicion of appendicitis but later confirmed not to have appendicitis. Information on appendicectomy versus conservative treatment with antibiotics was provided using a fixed script and standard information leaflet. Questionnaires covered factors influencing decisions, opinions regarding research, treatment preference and demographic data. We excluded parents not fluent in English. Of 113 respondents, 71(62.8%) chose antibiotics, 39(34.5%) chose appendicectomy, and 3(2.7%) had no preference. Reasons given for choosing antibiotics were fear of surgical risks and preferring less invasive treatment. Those choosing appendicectomy expressed preference for definitive treatment and fear of recurrence. Majority were against randomisation (n = 89, 78.8%) and blinding (n = 90, 79.7%). Over half found difficulty involving their child in research (n = 65, 57.5%). Most thought that research is important (66.4%) and beneficial to others (59.3%). Parents who perceived their child as healthy found research riskier (p = 0.039). Educated parents were more likely to find research beneficial to others (p = 0.012) but less accepting of randomisation (p = 0.001).Conclusion: More parents appear to prefer conservative treatment for acute uncomplicated appendicitis. Researchers must consider parental concerns regarding randomisation and blinding.What is Known:• Conservative management of acute uncomplicated appendicitis in paediatric patients is safe and effective, sparing the child the need for an operation; however, neither conservative nor surgical management is proven to be superior.• Randomised controlled trials provide the highest level of evidence, but it is challenging to recruit paediatric patients as participants in such clinical trials.What is New:• More parents prefer conservative management of uncomplicated appendicitis over surgical management for their children due to fear of surgical risks and complications• Randomisation in trial design is significantly associated with a parent's decision to reject their child's participation in a clinical trial.
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http://dx.doi.org/10.1007/s00431-019-03555-wDOI Listing
May 2020

Inhibition of miR‑155‑5p attenuates the valvular damage induced by rheumatic heart disease.

Int J Mol Med 2020 Feb 6;45(2):429-440. Epub 2019 Dec 6.

Department of Cardiology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.

Autoimmunity is involved in the valvular damage caused by rheumatic heart disease (RHD). Increased evidence has linked microRNAs (miRNAs/miRs) to autoimmune disease. Signal transducer and activator of transcription 3 (STAT3) and sphingosine‑1‑phosphate receptor 1 (S1PR1) and suppressor of cytokine signaling 1 (SOCS1) have been widely studied for their roles in autoimmunity and inflammation. Thus, the current study aims to investigate the role played by miR‑155‑5p in RHD‑induced valvular damage via the S1PR1, SOCS1/STAT3 and interleukin (IL)‑6/STAT3 signaling pathways. An RHD rat model was induced by inactivated Group A streptococci and complete Freund's adjuvant. A recombinant adeno‑associated virus (AAV‑miR155‑inhibitor) was used to inhibit the expression of miR‑155‑5p in the heart. Inflammation and fibrosis were assessed by hematoxylin and eosin staining and Sirius red staining. The expression of miR‑155‑5p in valvular tissues and serum exosomes was detected by reverse transcription‑quantitative PCR. S1PR1, SOCS1, STAT3, phosphorylated STAT3, IL‑6 and IL‑17 protein expression was detected by western blotting and immunohistochemistry. The relationships between miR‑155‑5p and S1PR1 and SOCS1 were detected by dual luciferase assays. Cytokine concentrations were measured by ELISA. The expression of miR‑155‑5p in valve tissues and serum exosomes was increased along with decreased S1PR1 and activated SOCS1/STAT3 signaling in the RHD model. The expression of IL‑6 and IL‑17 was increased in the valves and the serum. Dual luciferase assays showed that miR‑155‑5p directly targeted S1PR1 and SOCS1. Inhibition of valvular miR‑155‑5p through AAV pretreatment increased S1PR1 expression and inhibited activation of the SOCS1/STAT3 signal pathway as a result of attenuated valvular inflammation and fibrosis as well as a decrease in IL‑6 and IL‑17 in the valves and serum. These results suggest that inhibition of miR‑155‑5p can reduce RHD‑induced valvular damage via the S1PR1, SOCS1/STAT3 and IL‑6/STAT3 signaling pathways.
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http://dx.doi.org/10.3892/ijmm.2019.4420DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6984794PMC
February 2020

Engineering gelatin-based alginate/carbon nanotubes blend bioink for direct 3D printing of vessel constructs.

Int J Biol Macromol 2020 Feb 21;145:262-271. Epub 2019 Dec 21.

State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China. Electronic address:

Nowadays 3D bioprinting, due to its high structural reconstruction and low cost, has been a promising technology and gained expectation in the treatment of vascular diseases. Although some studies have reported that 3D printing of large-sized blood vessels in the human body has been achieved, there are still some problems to be solved urgently, such as the unfulfilled microvascular simulation and inferior biocompatibility and mechanical strength of scaffold materials. In this paper, the hybrid bioink prepared with gelatin, sodium alginate and carbon nanotubes were manufactured into cylindrical scaffolds through the collaboration between the vertical directional extrusion of printing nozzle and axial rotation of stepper motor module. Mouse epidermal fibroblasts were inoculated into the inner and outer walls of hollow tubular scaffolds to fabricate engineered blood vessels. The internal diameters of the bionic circular tubes printed in batches were 3 mm with an average wall thickness of 0.5 mm and a length of 7-10 cm. Results demonstrated that the proper doping of carbon nanotubes could effectively increase the mechanical properties of the composite scaffolds. Also, quantitative experiments proved that a small amount of doping of carbon nanotubes had little effect on cytotoxicity, and the constructs could meet the requirements of biomimetic vascular.
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http://dx.doi.org/10.1016/j.ijbiomac.2019.12.174DOI Listing
February 2020

Two Pathways Underlying the Effects of Physical Education on Out-of-School Physical Activity.

Res Q Exerc Sport 2020 Jun 24;91(2):197-208. Epub 2019 Oct 24.

University of North Carolina at Greensboro.

One primary goal of physical education (PE) is to promote students' lifelong physical activity (PA). This goal implies that PE should not only improve students' PA in PE classes but also promote their PA outside of the school known as the "PE effect." : In this study, we proposed a two-pathway model of the "PE effect" and hypothesized that learning in PE and positive motivational experience in PE are two possible pathways through which the "PE effect" emerges. We tested the tenability of this two-pathway model from the perspective of knowledge learning and autonomous motivation in PE. : A total of 394 eighth-grade students from five schools provided data on PA knowledge, out-of-school PA, and autonomous motivation toward PE and PA. Structural equation modeling was used to test the two-pathway model. : Results showed that the students' knowledge had a direct effect (β = .18, < .05) on their autonomous motivation for PA and an indirect effect (β = .02, < .05) on out-of-school PA through influencing autonomous motivation toward PA. Students' autonomous motivation for PE had a direct effect (β = .41, < .05) on their autonomous motivation toward PA and an indirect effect (β = .04, < .05) on out-of-school PA through autonomous motivation toward PA. : These results indicate that the two-pathway model is tenable in terms of knowledge learning and autonomous motivation in PE and imply that teaching knowledge in an autonomy-supportive PE environment may be an effective way to promote students' out-of-school PA.
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http://dx.doi.org/10.1080/02701367.2019.1656325DOI Listing
June 2020

[Clinical Value of Peripheral Blood Circulating Tumor Cells Detection in Early Cervical Infiltrating Carcinoma Screening].

Sichuan Da Xue Xue Bao Yi Xue Ban 2019 May;50(3):415-419

Department of Pathology, Zhongshan Boai Hospital Affiliated to Southern Medical University, Zhongshan 528400, China.

Objective: To observe the difference of circulating tumor cells (CTC) in peripheral blood of patients with different degrees of cervical lesions, and to evaluate the effectiveness of CTC detection in screening early invasive cervical cancer.

Methods: From December 2015 to October 2017, 63 cases of cervicitis, low-grade and high-grade intraepithelial lesions (LSIL, HSIL) and early invasive cervical cancer were confirmed by histopathological and clinical stages in Zhongshan Boai Hospital and Zhongshan Hospital Affiliated to Zhongshan University. The immunomagnetic bead negative enrichment technique combined with immunofluorescence was used. hybridization (imFISH) was used to detect CTC in peripheral blood of patients. The positive rate and quantity of CTC in four groups were analyzed. The diagnostic efficacy of CTC in early invasive cervical cancer was evaluated based on the results of histopathological diagnosis and clinical staging.

Results: ①The positive rates of CTC in cervicitis group, LSIL group, HSIL group and early invasive cervical cancer group were 0, 0, 19.05% and 84.13% respectively. The overall difference was statistically significant ( =504.00, <0.05). The positive rate of CTC in early invasive cervical cancer group was higher than that in other groups ( <0.008 3). The positive rates of CTC in HSIL group were significantly different from those in LSIL group and cervicitis group ( <0.008 3). ②The average number (median) of CTC positive in cervicitis group, LSIL group, HSIL group and early invasive cervical cancer group was 0, 0, 1/4 mL, 3/4 mL, respectively. The average number of positive CTC in early invasive cervical cancer group was higher than that in other groups, and the difference was significant compared with other groups ( <0.008 3). The average number of CTC positive in HSIL group was significantly different from that in LSIL and cervicitis group ( <0.008 3). ③The sensitivity, specificity, coincidence rate, positive predictive value and negative predictive value of CTC positive results in the diagnosis of early invasive cervical cancer were 84.13%, 93.65%, 91.27%, 81.54% and 94.65%, respectively.

Conclusion: CTC exists in patients with HSIL and early invasive cervical cancer. With the aggravation of cervical lesions, the positive rate and number of CTC test results increase. CTC detection in early invasive cervical cancer screening has a certain practical value in clinic.
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May 2019