Publications by authors named "Andrea Carolina Entrocassi"

5 Publications

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inhibitory effect of Lam. extracts over and on different stages of the chlamydial life cycle.

Heliyon 2021 May 18;7(5):e06947. Epub 2021 May 18.

Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Cátedra de Microbiología Clínica, Ciudad Autónoma de Buenos Aires, Argentina.

Chlamydial infections in humans are widely distributed and are responsible for a variety of acute and chronic diseases. Both and can lead to chronic conditions that have been linked to complications and sequelae. This study aimed to develop a culture method in order to detect antichlamydial activity of different extracts obtained from native Argentinian plants used as antimicrobials in local ethnomedicine and to evaluate their inhibitory activity over and growth. The inhibitory activity over different stages of the chlamydial life cycle on cell culture was assessed: the entry, the inclusion developing after entry, and the exponential growth stage. Also, the capability of rendering the cell refractory to chlamydial infection by pre-incubation with the extracts was assayed. Inhibitory activity of water-based and organic-based extracts obtained from Lam. (Araliaceae), (Vell.) Engl. (Anacardiaceae) and (Mutis ex L.f.) Baill. (Violaceae) were tested against five strains of (L2/434/BU and four clinical isolates form both neonatal conjunctivitis and adult genital infections, genotypes D, E, and K) and against AR39. The dichloromethane extract showed a broad inhibitory activity over the exponential growth stage of and independently from the chlamydial strain and the cell line. These results suggest a high inhibitory potential on both species. In order to characterize the dichloromethane active extract, an H-NMR was performed. The H-NMR characterization showed a spectrum with characteristic signals of the fatty acid moiety of lipids or cerebrosides, volatile phenolics, phytosterols, methyl triterpenes signals, and glucose moiety of the cerebrosides.
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http://dx.doi.org/10.1016/j.heliyon.2021.e06947DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8141889PMC
May 2021

Rectal Lymphogranuloma Venereum, Buenos Aires, Argentina.

Emerg Infect Dis 2019 03;25(3):598-599

Among 34 men with proctitis in Buenos Aires, Argentina, 16 (47%) had Chlamydia trachomatis infection, 11 (68.8%) of which were biovar lymphogranuloma venereum. The outbreak was probably local, as in Europe. In Argentina, lymphogranuloma venereum should be a suspected cause of proctitis in HIV-infected men who have had unprotected anal sex with men.
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http://dx.doi.org/10.3201/eid2503.180600DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6390746PMC
March 2019

Optimization of Trichomonas vaginalis Diagnosis during Pregnancy at a University Hospital, Argentina.

Korean J Parasitol 2016 Apr 30;54(2):191-5. Epub 2016 Apr 30.

Clinical Bacteriology Laboratory, Department of Clinical Biochemistry, Hospital de Clínicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.

The aim of this study was to evaluate different methods for Trichomonas vaginalis diagnosis during pregnancy in order to prevent maternal and perinatal complications. A total of 386 vaginal exudates from pregnant women were analyzed. T. vaginalis was investigated by 3 types of microscopic examinations direct wet mount with physiologic saline solution, prolonged May-Grunwald Giemsa (MGG) staining, and wet mount with sodium-acetate-formalin (SAF)/methylene blue method. PCR for 18S rRNA gene as well as culture in liquid medium were performed. The sensitivity and specificity of the microscopic examinations were evaluated considering the culture media positivity or the PCR techniques as gold standard. The frequency of T. vaginalis infection was 6.2% by culture and/or PCR, 5.2% by PCR, 4.7% by culture, 3.1% by SAF/methylene blue method and 2.8% by direct wet smear and prolonged MGG staining. The sensitivities were 83.3%, 75.0%, 50.0%, and 45.8% for PCR, culture, SAF/methylene blue method, and direct wet smear-prolonged MGG staining, respectively. The specificity was 100% for all the assessed methods. Microscopic examinations showed low sensitivity, mainly in asymptomatic pregnant patients. It is necessary to improve the detection of T. vaginalis using combined methods providing higher sensitivity, such as culture and PCR, mainly in asymptomatic pregnant patients, in order to prevent maternal and perinatal complications.
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http://dx.doi.org/10.3347/kjp.2016.54.2.191DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4870974PMC
April 2016

Isolation of Chlamydophila pneumoniae from atheromas of the carotid artery and their antibiotics susceptibility profile.

Enferm Infecc Microbiol Clin 2006 Feb;24(2):81-5

Instituto de Virología Dr. J.M. Vanella, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Argentina.

Background: Atherosclerosis is pathogenically similar to a chronic inflammatory response. Peripheral arterial disease (PAD) is a common manifestation of atherosclerosis. Chlamydophila pneumoniae has been suggested to play a role in the origin of PAD.

Objective: To determine whether C. pneumoniae is present in atherosclerosis lesions of the carotid artery wall in patients with PAD through several diagnostic methods and to characterize C. pneumoniae susceptibility profiles.

Methods: The presence of C. pneumoniae in 9 tissue samples from atherosclerotic lesions obtained by carotid endarterectomy was investigated by 3 methods. Karnofsky-fixed specimens were examined by transmission electron microscopy (TEM), isolation of C. pneumoniae was attempted in LLCMK2 cell structure (ICC), and the presence of chlamydial DNA was investigated by polymerase chain reaction (PCR). The in vitro activities of azithromycin, roxithromycin and penicillin were tested in 4 isolations and the reference strain of C. pneumoniae (AR39).

Results: C. pneumoniae was detected in atherosclerotic plaques from 4 patients with PAD. The pathogen was identified by TEM, PCR and ICC. We report data of the in vitro susceptibility of 4 strains. These strains did not differ from respiratory AR39 strain in their susceptibility patterns to azithromycin, roxithromycin and penicillin.

Conclusions: C. pneumoniae is frequently found in the advanced carotid atherosclerotic lesions of patients undergoing endarterectomy. Although these findings do not establish causality in carotid artery atherosclerosis, they should stimulate investigation of the possible causal or pathogenic role of C. pneumoniae. Notably, the profiles of antibiotic susceptibility of C. pneumoniae isolated from 4 of the patients did not differ from those of the reference strain.
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http://dx.doi.org/10.1157/13085013DOI Listing
February 2006
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