Publications by authors named "Anastasia A Gorokhova"

2 Publications

  • Page 1 of 1

The localization of the photosensitizer determines the dynamics of the secondary production of hydrogen peroxide in cell cytoplasm and mitochondria.

J Photochem Photobiol B 2021 Jun 6;219:112208. Epub 2021 May 6.

Lobachevsky State University of Nizhny Novgorod, 23 Gagarin ave., Nizhny Novgorod 603950, Russia. Electronic address:

Photodynamic therapy (PDT) is based on the production of the cytotoxic reactive oxygen species (ROS) by light irradiation of a photosensitizer dye in the presence of molecular oxygen. Along with photochemical ROS production, it becomes evident that PDT induces massive secondary production of ROS which is registered long after the irradiation is completed. We created cell lines of human epidermoid carcinoma with the cytoplasmic and mitochondrial localization of protein sensor HyPer sensitive to hydrogen peroxide to compare its concentration in two cellular compartments. The lag-period between irradiation and accumulation of hydrogen peroxide in cells was registered; its duration was dose-dependent and increased up to 80 min when lowering the exposition dose from 50 to 15 J/cm. We have shown that localization of the photosensitizer determines the spatiotemporal pattern of the cell response to PDT: secondary hydrogen peroxide accumulation in cell cytoplasm induced by photodynamic treatment with lysosome-localized phtalocyianine Photosens occurs several minutes prior to that in mitochondria; on the contrary, membranotropic arylcyanoporphyrazine dye leads to massive mitochondrial hydrogen peroxide production followed by its cytoplasmic accumulation. We hypothesize that photosensitizers with various physicochemical properties and intracellular localization can trigger different patterns not only of primary but also secondary ROS production leading to different cell fate outcomes.
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http://dx.doi.org/10.1016/j.jphotobiol.2021.112208DOI Listing
June 2021

Monitoring of hydrogen peroxide production under photodynamic treatment using protein sensor HyPer.

J Photochem Photobiol B 2018 Jan 17;178:296-301. Epub 2017 Nov 17.

Lobachevsky State University of Nizhny Novgorod, 23 Gagarin ave., Nizhny Novgorod 603950, Russia. Electronic address:

An interest to HO accumulation under photodynamic treatment can be explained by its participation in intracellular signal cascades. It is important not only to detect HO generation, but also to trace the dynamics of its intracellular content. In the present study the dynamics of cellular HO content under photodynamic treatment was analyzed using genetically encoded reversible HO-sensitive sensor HyPer. Real-time detecting of HO production after photodynamic treatment was performed using the protein sensor and individual features of action of different photosensitizers were revealed. Photodynamic treatment with a number of chlorin and phthalocyanine photosensitizers was found to induce secondary production of HO in the cells. Three types of dynamic responses were registered: monotonous increase of HO level during the entire observation time in the presence of Fotoditazin and Holosens; transient short-term accumulation in the presence of Radachlorin and Phthalosens; and relatively low-level stable increase in the presence of Photosens. The listed photosensitizers differ significantly in intracellular localization and physicochemical properties, which can determine the differences in the response of HO after the photodynamic treatment. In general, it has been shown that the rapid transient HO response is typical for hydrophobic compounds localized in membrane cell structures, whereas in the presence of more hydrophilic dyes a prolonged monotonous HO accumulation occurs.
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http://dx.doi.org/10.1016/j.jphotobiol.2017.11.020DOI Listing
January 2018