Publications by authors named "Ana Torre"

68 Publications

Xanthogranulomatous salpingo-oophoritis presenting as an ovarian tumour.

BMJ Case Rep 2022 May 19;15(5). Epub 2022 May 19.

Gynecology, Centro Hospitalar de Vila Nova de Gaia Espinho EPE, Vila Nova de Gaia, Portugal.

Xanthogranulomatous salpingo-oophoritis (XGSO) is an exceptionally rare entity. Its clinical manifestations and imaging modalities can mimic benign and malignant adnexal diseases, making it difficult to diagnose. Here we report a case of XGSO in a young woman who was operated with suspicion of a borderline ovarian tumour. Preoperative diagnosis of XGSO should be considered to avoid radical surgical treatment, especially in young patients.
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http://dx.doi.org/10.1136/bcr-2021-248197DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9121422PMC
May 2022

Analysis of Specific Physical Fitness in High-Level Table Tennis Players-Sex Differences.

Int J Environ Res Public Health 2022 04 22;19(9). Epub 2022 Apr 22.

ENFYRED Research Group, Faculty of Health and Sports Sciences, University of Zaragoza, 22001 Huesca, Spain.

Table tennis performance depends on multiple factors such as technique, tactics and fitness. Several studies have focused on investigating different technical-tactical variables. However, research analysing the specific physical qualities of this sport is scarce, particularly in the female sex. The aim of the present study was to assess the physical fitness variables associated with individual performance in elite table tennis players according to sex. Forty-eight elite players divided into males ( = 24; 25.38 ± 4.01 years) and females ( = 24; 22.33 ± 3.83 years) participated in the study. To determine physical fitness, participants performed vertical jump, hand grip strength, ergospirometry and lateral displacement tests (reaction time, displacement time and lateral acceleration). Male players showed higher values in vertical jump, hand grip strength and maximum oxygen consumption ( < 0.001). Likewise, male players moved laterally faster ( < 0.001). On the other hand, female players had a better reaction time towards the dominant side ( < 0.01). Elite male table tennis players showed better physical fitness compared to female players. Due to the scarcity of data on elite table tennis players, these results can serve as reference values for different table tennis practitioners.
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http://dx.doi.org/10.3390/ijerph19095119DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9105729PMC
April 2022

Identifying the spatiotemporal vulnerability of soils to antimicrobial contamination through land application of animal manure in Minnesota, United States.

Sci Total Environ 2022 Aug 6;832:155050. Epub 2022 Apr 6.

Department of Veterinary and Biomedical Sciences, University of Minnesota, 1971 Commonwealth Ave, Falcon Heights, MN 55108, United States.

Antimicrobials may reach the soil environment from a variety of sources and pathways, including land application of human biosolids and animal manure. Once in soil, antimicrobials can affect the abundance and activity of soil microorganisms and exert selection pressures that enhance the emergence and spread of antimicrobial resistance (AMR). To mitigate the spread of AMR it is important to understand the spatial and temporal interactions between antimicrobials and soil. The goal of this study was to assess the vulnerability of Minnesota (U.S.) soil to contamination with specific antimicrobial compounds at temperatures experienced throughout the year. Soil contamination potential was estimated based upon specific antimicrobial drug binding and permanence, and average monthly temperature. Minnesota soil vulnerability was estimated by incorporating spatially explicit soil contamination potential, land cover type, and livestock density. Assessment of antimicrobials used in livestock production showed that soils are most vulnerable to antimicrobial contamination in southwestern Minnesota, to enrofloxacin, chlortetracycline, and oxytetracycline, and in the months of April and October. While the assessment herein was not based on actual on-farm antimicrobial use data and subsequent excretion of antimicrobial metabolites into the environment, this study provides an overview of the spatial and temporal potential for Minnesota soil to be contaminated by several antimicrobial drugs and demonstrates how specific vulnerability assessments might be conducted for geographic areas with known exposure (e.g., cropland fertilized with livestock manure and/or human biosolids). Such assessments might be used to identify best practices for mitigating antimicrobial exposure to soils and guide additional research to understand the role of environmental antimicrobial contamination in the problem of AMR.
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http://dx.doi.org/10.1016/j.scitotenv.2022.155050DOI Listing
August 2022

Severe blistering eruptions induced by immune checkpoint inhibitors: a multicentre international study of 32 cases.

Melanoma Res 2022 06 29;32(3):205-210. Epub 2022 Mar 29.

European Task Force 'Dermatology for Cancer Patients', EADV, Toulouse.

Among dermatologic adverse events induced by immune checkpoint inhibitors (ICI), bullous life-threatening reactions are rare. To better define the clinical and histological features, treatment, and prognosis of ICI-related severe blistering cutaneous eruptions. This retrospective case series was conducted between 2014/05/15 and 2021/04/15 by the dermatology departments of four international registries involved in drug reactions. Inclusion criteria were age ≥18 years old, skin eruption with blisters with detachment covering ≥1% body surface area and at least one mucous membrane involved, available pictures, and ICI as suspect drug. Autoimmune bullous disorders were excluded. Each participant medical team gave his own diagnosis conclusion: epidermal necrolysis (EN), severe lichenoid dermatosis (LD), or unclassified dermatosis (UD). After a standardized review of pictures, cases were reclassified by four experts in EN or LD/UD. Skin biopsies were blindly reviewed. Thirty-two patients were included. Median time to onset was 52 days (3-420 days). Cases were originally diagnosed as EN in 21 cases and LD/UD in 11 cases. After review by experts, 10/21 EN were reclassified as LD/UD. The following manifestations were more frequent or severe in EN: fever, purpuric macules, blisters, ocular involvement, and maximal detachment. Most patients were treated with topical with or without systemic corticosteroids. Eight patients (25%) died in the acute phase. The culprit ICI was not resumed in 92% of cases. In three patients, another ICI was given with a good tolerance. Histology did not reveal significant differences between groups. Severe blistering cutaneous drug reactions induced by ICI are often overdiagnosed as EN. Consensus for management is pending.
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http://dx.doi.org/10.1097/CMR.0000000000000819DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9377568PMC
June 2022

Subclinical Cushing's syndrome: resection of adrenal incidentaloma.

BMJ Case Rep 2022 03 29;15(3). Epub 2022 Mar 29.

Department of General Surgery, Centro Hospitalar de Vila Nova de Gaia Espinho EPE, Vila Nova de Gaia, Portugal.

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http://dx.doi.org/10.1136/bcr-2021-247600DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8966519PMC
March 2022

Hand, foot, and mouth disease in adults caused by Coxsackievirus B1-B6.

An Bras Dermatol 2022 May-Jun;97(3):321-325. Epub 2022 Mar 7.

Department of Dermatology, Hospital Italiano de Buenos Aires, Buenos Aires, Argentina.

Hand, foot, and mouth disease is a viral rickettsial disease caused by Coxsackievirus A16 and Enterovirus 71 in most cases. It is commonly seen in children under ten years old, who present oral enanthema and a macular, maculopapular, or vesicular rash on their hands and feet. However, an increase in cases caused by other viral serotypes was observed in adults in recent years with various clinical presentations and a troublesome diagnosis. Three cases of hand, foot, and mouth disease are reported to show the clinical variability and diagnostic complexity that this disease may present in adult patients.
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http://dx.doi.org/10.1016/j.abd.2021.03.012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9133263PMC
May 2022

African Swine Fever Survey in a European Context.

Pathogens 2022 Jan 23;11(2). Epub 2022 Jan 23.

Infectious Diseases and Global Health Department, Centro de Investigación en Sanidad Animal (CISA), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria-Consejo Superior de Investigaciones Científicas (INIA-CSIC), 28130 Madrid, Spain.

African swine fever (ASF) is currently the most threatening disease for domestic and wild pigs worldwide. Wild boar has been the main affected species in all EU countries except for Romania, where most notifications occur in domestic pigs. The spread of ASF in wild boar is challenging to control; risk factors are harder to identify and establish than in domestic pigs, which, together with an underestimation of the disease and the lack of treatment or an effective vaccine, are hindering control and eradication efforts. We distributed two online questionnaires, one for domestic pigs and one for wild boar, to experts of different background and countries in Europe, to explore risk factors in relation to ASF control connected to farming, hunting, trade, the environment, and domestic pig and wild boar populations. Overall, wild boar movements were estimated to pose the highest risk of ASF introduction and spread. The movement of pork and pork products for own consumption also ranked high. Here we explored, in addition to the assessment of risk pathways, the identification of risks of transmission at the domestic/wild boar interface, the importance of biosecurity practices and improved control efforts, and controversial opinions that require further attention.
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http://dx.doi.org/10.3390/pathogens11020137DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8878522PMC
January 2022

Caecum volvulus as a late presentation of intestinal malrotation in an adult: a challenging diagnosis.

BMJ Case Rep 2022 Feb 2;15(2). Epub 2022 Feb 2.

General Surgery Department, Centro Hospitalar Vila Nova de Gaia Espinho EPE, Vila Nova de Gaia, Portugal.

Intestinal malrotation is usually diagnosed in early childhood. It results from failure of the normal gut rotation during embryological development. We present a case of a 62-year-old woman with a delayed presentation of an intestinal malrotation. She was admitted in the emergency department with an acute intestinal obstruction. Exploratory laparotomy revealed Ladd's band with caecum volvulus and intestinal malrotation. Ladd's procedure and right hemicolectomy were performed with uneventful recovery. Since both caecum volvulus and intestinal malrotation are rare events, particularly in adulthood, clinical diagnosis is challenging. Our aim is to increase the awareness of surgeons about this rare association as a cause of acute intestinal obstruction.
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http://dx.doi.org/10.1136/bcr-2021-247645DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8811584PMC
February 2022

Prevalence of atopic dermatitis in adults.

An Bras Dermatol 2022 Jan-Feb;97(1):107-109. Epub 2021 Nov 26.

Adults and Pediatrics Allergy Units, Hospital Italiano de Buenos Aires, Buenos Aires, Argentina.

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http://dx.doi.org/10.1016/j.abd.2020.10.016DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8799860PMC
February 2022

Physiological Profile, Metabolic Response and Temporal Structure in Elite Individual Table Tennis: Differences According to Gender.

Int J Environ Res Public Health 2021 11 12;18(22). Epub 2021 Nov 12.

School of Sport Sciences, University of Extremadura, 10003 Cáceres, Spain.

No research that has analyzed the structural characteristics, physiological profile, and energy demands in the game of table tennis as played by women is available. The present study aimed to evaluate the physiological, metabolic, and temporal variables of table tennis players and to observe gender differences. Forty-eight elite table tennis players participated in this study: 24 men (25.3 ± 4.07 years) and 24 women (22.3 ± 3.8 years). During simulated competition, temporal structure, heart rate (HR), and lactate (LA) were evaluated. The maximum ergospirometric evaluations were performed in a laboratory. The total table tennis (TT) time and the total resting time (TRT) were longer for men ( < 0.05), but game density was higher for women ( < 0.05). During rallies, the real playing time (RPT) was longer for women, while the TRT was longer for men ( < 0.05). The maximum HR, minimum HR, and maximum LA concentrations were higher for men ( < 0.05). The obtained data reveal gender differences in the physiological, metabolic, structural, and temporal variables in table tennis players. The analysis of the studied variables could allow training sessions to be planned and organized according to table tennis players' gender.
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http://dx.doi.org/10.3390/ijerph182211898DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8623913PMC
November 2021

Editorial: Antimicrobials in Wildlife and the Environment.

Front Microbiol 2021 5;12:783118. Epub 2021 Nov 5.

The School of Medicine, Medical Sciences and Nutrition, University of Aberdeen, Aberdeen, United Kingdom.

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http://dx.doi.org/10.3389/fmicb.2021.783118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8602900PMC
November 2021

Phytotoxic Effects of Antibiotics on Terrestrial Crop Plants and Wild Plants: A Systematic Review.

Arch Environ Contam Toxicol 2022 Jan 20;82(1):48-61. Epub 2021 Oct 20.

Animal Health Research Centre, National Institute for Agricultural and Food Research and Technology (INIA), Valdeolmos, Madrid, Spain.

This review examines the state of knowledge on the phytotoxic effects of antibiotics on terrestrial crop plants and wild (non-crop) plants with the goal of evaluating differences in their sensitivity. This is important because environmental risk assessments of antibiotics currently consider their potential effects only on crop species but not wild species. Overall, we analysed 275 datasets consisting of antibiotic-plant species-endpoint combinations for germination (mg/L) and 169 datasets for plant growth (elongation and biomass) (mg/kg). EC10 and EC50 of each parameter were compared using a quotient approach, in which the geometric mean and the 5th percentile of the crop data were divided by wild data. Quotients were > 1 for elongation growth, suggesting that wild species were more sensitive than crops, while they were < 1 for biomass growth, suggesting quite the contrary. However, < 1% of the data in each dataset came from wild species, preventing definitive conclusions. Merging crop and wild data to evaluate differences in sensitivity among classes of antibiotics and plant families, we found using a linear mixed effect model and post hoc test that plants were most sensitive to phenicol and least sensitive to macrolides and tetracyclines. Further work must be conducted to gain a better understanding of the phytotoxic effects of antibiotics on terrestrial wild plants and subsequently assess whether the current approach to environmental risk assessment of antibiotics is sufficient to protect plant biodiversity.
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http://dx.doi.org/10.1007/s00244-021-00893-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8732949PMC
January 2022

[Dermatological emergencies. Prospective and multicentric study in Argentina].

Medicina (B Aires) 2021 ;81(4):546-554

Servicio de Dermatología, Hospital Provincial del Centenario, Rosario, Santa Fe, Argentina.

Dermatological emergencies are a frequent reason for emergency departments consultation. In order to determine the prevalence of dermatological emergencies, to describe the kind of dermatological diseases that present as emergencies, to analyze the coincidence between the diagnoses received by the patients in those cases with a previous consultations for the same cutaneous manifestation, and to analyze the behavior according to the health system segment in which they were attended: public segment and private segment; a prospective, observational, analytical, cross-sectional and multi-center study was carried out. Two thousand eight hundred one patients were included. The prevalence of consultations for dermatological emergencies in adults was 15% in the same time period (public segment: 10.6 and private segment: 22.5%, p < 0.05). The consultation was due to an infectious disease in 35.5%, allergic in 29.6% and neoplastic in 8.6%; 0.7% of patients were hospitalized. In 31.7% of patients who had a previous consultation, a coincidence was found between the diagnoses made in 80.7% of those attended by a dermatologist, and 52.6% evaluated by non-dermatologist physician. The high prevalence of dermatological consultations and the existing differences in the probability of receiving an appropriate diagnosis according to the specialization of the intervening professional, show the importance of the presence of dermatology-trained physicians in the emergency area.
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August 2021

[Validation of a Spanish questionnaire on telemedicine usability].

Medicina (B Aires) 2020 ;80(6):649-653

Servicio de Dermatología, Hospital Italiano de Buenos Aires, Argentina. E-mail:

Telemedicine is the provision of services by health professionals through the use of information and communication technologies. Its implementation is usually carried out through systems of va riable usability. In 2016, Parmanto et al, developed and validated a questionnaire in English that measures its usability and allows to evaluate all factors that influence it. In 2019, we translated, adapted cross-culturally, and published this questionnaire in Spanish. With the aim of transforming it into a reliable and accessible tool, the utility of which is indisputable in the current context, we carried out a research work that allowed its validation and simplification.
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December 2020

Spatial Trends in Infection in Pigs in Spain.

Front Vet Sci 2020 23;7:345. Epub 2020 Jun 23.

VISAVET Health Surveillance Center, Universidad Complutense, Madrid, Spain.

is one of the most important foodborne pathogens worldwide. Its main reservoirs are poultry and pigs, in which infection is endemic in many countries. Spain has one of the largest pig populations in the world. Even though infection is commonly detected in pig farms, its spatial distribution at the national level is poorly understood. Here we aimed to report the spatial distribution of -positive pig farms in Spain and investigate the presence of potential spatial trends over a 17-year period. For this, data on samples from pigs tested for in 2002-2013, 2015, 2017, and 2019 as part of the Spanish Veterinary Antimicrobial Resistance Surveillance program, representing 3,730 farms were analyzed. The spatial distribution and clustering of -positive pig farms at the province level were explored using spatial empirical Bayesian smoothing and global Moran's , local Moran's , and the Poisson model of the spatial scan statistics. Bayesian spatial regression using a reparameterized Besag-York-Mollié Poisson model (BYM2 model) was then performed to quantify the presence of spatially structured and unstructured effects while accounting for the effect of potential risk factors for infection at the province level. The overall proportion of -positive farms was 37.8% (95% confidence interval: 36.2-39.4). Clusters of positive farms were detected in the East and Northeast of Spain. The Bayesian spatial regression revealed a West-to-East increase in the risk of infection at the province level, with 65.2% (50% highest density interval: 70-100.0%) of this spatial pattern being explained by the spatially structured component. Our results demonstrate the existence of a spatial variation in the risk of infection in pig farms at the province level in Spain. This information can help to optimize risk-based surveillance programs in Spain, although further research to identify farm-level factors explaining this pattern are needed.
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http://dx.doi.org/10.3389/fvets.2020.00345DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7325609PMC
June 2020

Acute generalized exanthematous pustulosis with features mimicking toxic epidermal necrolysis secondary to amiodarone.

An Bras Dermatol 2020 Jul - Aug;95(4):540-542. Epub 2020 May 11.

Department of Dermatology, Hospital Italiano de Buenos Aires, Buenos Aires, Argentina.

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http://dx.doi.org/10.1016/j.abd.2019.11.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7335889PMC
July 2020

Assessing the benefits of composting poultry manure in reducing antimicrobial residues, pathogenic bacteria, and antimicrobial resistance genes: a field-scale study.

Environ Sci Pollut Res Int 2020 Aug 12;27(22):27738-27749. Epub 2020 May 12.

Animal Health Research Center (INIA-CISA), Ctra Algete a El Casar s/n, 28130, Valdeolmos, Madrid, Spain.

The poultry industry in the European Union produces 13 million tons of manure annually, which represents a major health and environmental challenge. Composting is an environmental-friendly technique for the management of manure, but there are few studies about antibiotic residues and antimicrobial resistances at a field scale. The goal of this study was to determine if the composting of poultry manure at a field scale would result in the reduction of antibiotic residues, pathogenic bacteria, and antibiotic resistance genes (ARGs) in the final fertilizer product. A 10-week composting of poultry manure spiked with enrofloxacin, doxycycline, and ciprofloxacin was performed. The determination of antibiotics residues and 22 selected ARGs was carried out together with the identification of bacteria by metagenomics. In the case of ciprofloxacin and doxycycline, a 90% decrease was observed after composting for 3 weeks. Sixteen ARGs were detected at the beginning of the experiment; 12 of them decreased from week 0 to week 10 (reduction of 73.7-99.99%). The presence of potentially pathogenic bacteria, such as, Campylobacter coli or commensal bacteria such as Escherichia coli decreases along the composting process. In conclusion, 10-week composting of poultry manure promotes the reduction of antibiotic residues and most of the ARGs and pathogenic bacteria.
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http://dx.doi.org/10.1007/s11356-020-09097-1DOI Listing
August 2020

[Spanish translation and transcultural adaptation of a questionnaire on telemedicine usability].

Medicina (B Aires) 2020 ;80(2):134-137

Servicio de Dermatología, Hospital Italiano de Buenos Aires, Argentina. E-mail:

Telemedicine is the provision of services by health professionals, through the use of information and communication technologies. Its implementation helps to shorten distances, facilitate access to the health system, improve the effectiveness of care and reduce costs. In 2016, Bambang Parmanto et al, developed and validated a questionnaire in English that measures its usability and allows to evaluate all the factors that influence it. Since similar tools do not exist in the Spanish language to date, a research work was carried out for transcultural translation and adaptation that included seven steps. The questionnaire obtained after this process provides a valuable tool to objectify information that is vital to contribute to the cycle of continuous improvement of the care process of these characteristics, in which patients, health professionals and funders are involved.
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June 2020

Idiopathic left omental infarction.

Autops Case Rep 2020 Jan-Mar;10(1):e2019123. Epub 2019 Dec 13.

Centro Hospitalar Vila Nova de Gaia/Espinho. Vila Nova de Gaia, Portugal.

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http://dx.doi.org/10.4322/acr.2019.123DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6945327PMC
December 2019

Genetic characterization of Carnivore Parvoviruses in Spanish wildlife reveals domestic dog and cat-related sequences.

Transbound Emerg Dis 2020 Mar 22;67(2):626-634. Epub 2019 Oct 22.

Facultad de Ciencias de la Vida, Universidad Andres Bello, Santiago, Chile.

The impact of carnivore parvovirus infection on wild populations is not yet understood; disease signs are mainly developed in pups and assessing the health of litters in wild carnivores has big limitations. This study aims to shed light on the virus dynamics among wild carnivores thanks to the analysis of 213 samples collected between 1994 and 2013 in wild ecosystems from Spain. We determined the presence of carnivore parvovirus DNA by real-time PCR and sequenced the vp2 gen from 22 positive samples to characterize the strains and to perform phylogenetic analysis. The presence of carnivore parvovirus DNA was confirmed in 18% of the samples, with a higher prevalence detected in wolves (Canis lupus signatus, 70%). Fourteen sequences belonging to nine wolves, three Eurasian badgers (Meles meles), a common genet (Genetta genetta) and a European wildcat (Felis silvestris) were classified as canine parvovirus 2c (CPV-2c); five sequences from three wolves, a red fox (Vulpes vulpes) and a stone marten (Martes foina) as CPV-2b; and three sequences from a badger, a genet and a stone marten as feline parvovirus (FPV). This was the first report of a wildcat infected with a canine strain. Sequences described in this study were identical or very close related to others previously found in domestic carnivores from distant countries, suggesting that cross-species transmission takes place and that the parvovirus epidemiology in Spain, as elsewhere, could be influenced by global factors.
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http://dx.doi.org/10.1111/tbed.13378DOI Listing
March 2020

Real-life approach and treatment of chronic urticaria in Argentina: A National Survey.

Dermatol Ther 2019 11 7;32(6):e13086. Epub 2019 Oct 7.

Department of Dermatology, Hospital Italiano de Buenos Aires, Buenos Aires, Argentina.

The management of chronic urticaria (CU) has been controversial. Recently updated international guidelines propose evidence-based diagnosis and treatment. Nevertheless, dermatologists have heterogeneous approaches to managing CU. To estimate the percentage of dermatologists who have an optimal approach to CU according to the international guidelines, and to explore the variables associated with optimal management a cross-sectional study using a 17-question survey was delivered by email to dermatologists from Argentina. Optimal first, second, and third line treatment were considered when dermatologists chose a nonsedative antihistamine; increased the dosage of nonsedative antihistamines, and added omalizumab, respectively. Logistic regression was used to identify variables associated with optimal management. A total of 165 questionnaires were available for analysis. An optimal first, second, and third-line treatment approach was identified in 50%, 35%, and 15% of the dermatologists, respectively. The dermatologists' age being above 55 years old (OR: 0.12, 95% CI: 0.01-0.99, p .005) and having more than 5 years of expertise (OR: 0.4, 95% CI: 0.19-0.81, p .001) were significantly associated with a suboptimal approach in second-line treatment. We could not find variables associated with an optimal first or third-line treatment. The real-life management of CU in Argentina is partly suboptimal according to the international guidelines.
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http://dx.doi.org/10.1111/dth.13086DOI Listing
November 2019

The Incorporation of Extracellular Vesicles from Mesenchymal Stromal Cells Into CD34 Cells Increases Their Clonogenic Capacity and Bone Marrow Lodging Ability.

Stem Cells 2019 10 11;37(10):1357-1368. Epub 2019 Jun 11.

Servicio de Hematología, IBSAL-Hospital Universitario de Salamanca, Salamanca, Spain.

Mesenchymal stromal cells (MSC) may exert their functions by the release of extracellular vesicles (EV). Our aim was to analyze changes induced in CD34 cells after the incorporation of MSC-EV. MSC-EV were characterized by flow cytometry (FC), Western blot, electron microscopy, and nanoparticle tracking analysis. EV incorporation into CD34 cells was confirmed by FC and confocal microscopy, and then reverse transcription polymerase chain reaction and arrays were performed in modified CD34 cells. Apoptosis and cell cycle were also evaluated by FC, phosphorylation of signal activator of transcription 5 (STAT5) by WES Simple, and clonal growth by clonogenic assays. Human engraftment was analyzed 4 weeks after CD34 cell transplantation in nonobese diabetic/severe combined immunodeficient mice. Our results showed that MSC-EV incorporation induced a downregulation of proapoptotic genes, an overexpression of genes involved in colony formation, and an activation of the Janus kinase (JAK)-STAT pathway in CD34 cells. A significant decrease in apoptosis and an increased CD44 expression were confirmed by FC, and increased levels of phospho-STAT5 were confirmed by WES Simple in CD34 cells with MSC-EV. In addition, these cells displayed a higher colony-forming unit granulocyte/macrophage clonogenic potential. Finally, the in vivo bone marrow lodging ability of human CD34 cells with MSC-EV was significantly increased in the injected femurs. In summary, the incorporation of MSC-EV induces genomic and functional changes in CD34 cells, increasing their clonogenic capacity and their bone marrow lodging ability. Stem Cells 2019;37:1357-1368.
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http://dx.doi.org/10.1002/stem.3032DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6852558PMC
October 2019

Salmonella Surveillance Systems in Swine and Humans in Spain: A Review.

Vet Sci 2019 Feb 20;6(1). Epub 2019 Feb 20.

Animal Health Department, Veterinary School, Complutense University of Madrid, 28040 Madrid, Spain.

Non-typhoid salmonellosis is a common and problematic foodborne zoonotic disease in which pork and pork products can be an important potential source of infection. To prevent this disease, important efforts to monitor the situation in the main source, livestock, are conducted in most developed countries. In the European Union, European Food Safety Agency (EFSA) and European Center for Disease Control (ECDC) compile information at the member-state level, even though important differences in production systems and surveillance systems exist. Here, surveillance systems in one of the main sources of foodborne salmonellosis, swine, and humans in Spain were reviewed to identify potential gaps and discuss potential ways of integration under a "One-Health" approach. Despite the extensive information generated through the surveillance activities, source attribution can be only routinely performed through ad-hoc outbreak investigations, and national reports on human outbreaks do not provide sufficiently detailed information to gain a better understanding of the epidemiology of the pathogen. Human and animal monitoring of would benefit from a better exchange of information and collaboration. Analysis of spatio-temporal trends in livestock and humans could help to identify likely sources of infection and to target surveillance efforts in areas with higher prevalence or where specific strains are found.
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http://dx.doi.org/10.3390/vetsci6010020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6466228PMC
February 2019

Chagas disease reactivation: cutaneous manifestations in a transplanted patient.

An Bras Dermatol 2018 Nov/Dec;93(6):890-892

Infectology section, Department of Internal Medicine, Hospital Italiano de Buenos Aires, Buenos Aires, Argentina.

Chagas disease is an endemic zoonosis caused by a protozoan agent called Trypanosoma cruzi. It is mainly transmitted by a hematophagous vector, and less frequently by blood transfusion, transplacental and solid organ transplant. In most cases, primary infection is not diagnosed and the disease progresses to a chronic phase. Immunosuppressed patients are a vulnerable population that may present an acute, atypical and severe reactivation of the chronic form of this disease. We hereby report a case of a female patient, who received a renal transplant with immunosuppressive treatment, who was diagnosed with a chagasic hypodermitis secondary to an acute reactivation of a chronic phase of this disease. We describe the clinical features, epidemiological and histopathological findings, treatment and course.
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http://dx.doi.org/10.1590/abd1806-4841.20187855DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6256210PMC
February 2019

Bullous pyoderma gangrenosum as a predictor of hematological malignancies.

An Bras Dermatol 2018 Jan-Feb;93(1):133-134

Department of Dermatology at Universidad de Buenos Aires - Buenos Aires, Argentina.

Pyoderma gangrenosum may precede, coexist or follow diverse systemic diseases. The bullous variety is usually associated with hematologic disorders. From 31 patients with pyoderma gangrenosum diagnosed in our hospital during 10 years, only 2 presented with the bullous type. One patient had previous diagnosis of chronic myeloid leukemia. Both had fast-progressive, painful lesions, and both showed an excellent response to systemic corticosteroids. In less than 3 months the lesions recurred simultaneously with the progression of chronic myeloid leukemia in one patient and with the initial diagnosis of acute myeloid leukemia in the other one. They died in less than four weeks. These findings emphasize the importance of its timely diagnosis. Cutaneous lesions could be the first predictor of an underlying disease.
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http://dx.doi.org/10.1590/abd1806-4841.20187031DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5871381PMC
April 2018

Reactivation of Chagas-Mazza disease during treatment with infliximab.

An Bras Dermatol 2017 Nov-Dec;92(6):899-900

Department of dermatology, Hospital Italiano de Buenos Aires - Ciudad Autónoma de Buenos Aires, Argentina.

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http://dx.doi.org/10.1590/abd1806-4841.20177346DOI Listing
September 2018

Approaches and Perspectives for Development of African Swine Fever Virus Vaccines.

Vaccines (Basel) 2017 Oct 7;5(4). Epub 2017 Oct 7.

OIE Reference Laboratory for ASF, Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense de Madrid, Avda. Puerta del Hierro, Madrid 28040, Spain.

African swine fever (ASF) is a complex disease of swine, caused by a large DNA virus belonging to the family Asfarviridae. The disease shows variable clinical signs, with high case fatality rates, up to 100%, in the acute forms. ASF is currently present in Africa and Europe where it circulates in different scenarios causing a high socio-economic impact. In most affected regions, control has not been effective in part due to lack of a vaccine. The availability of an effective and safe ASFV vaccines would support and enforce control-eradication strategies. Therefore, work leading to the rational development of protective ASF vaccines is a high priority. Several factors have hindered vaccine development, including the complexity of the ASF virus particle and the large number of proteins encoded by its genome. Many of these virus proteins inhibit the host's immune system thus facilitating virus replication and persistence. We review previous work aimed at understanding ASFV-host interactions, including mechanisms of protective immunity, and approaches for vaccine development. These include live attenuated vaccines, and "subunit" vaccines, based on DNA, proteins, or virus vectors. In the shorter to medium term, live attenuated vaccines are the most promising and best positioned candidates. Gaps and future research directions are evaluated.
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http://dx.doi.org/10.3390/vaccines5040035DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5748602PMC
October 2017
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