Publications by authors named "Ana Carolina Guimarães Faleiros"

11 Publications

  • Page 1 of 1

Effects of cholinergic stimulation with pyridostigmine bromide on chronic chagasic cardiomyopathic mice.

Mediators Inflamm 2014 24;2014:475946. Epub 2014 Aug 24.

Natural and Biological Sciences Institute, Triangulo Mineiro Federal University, Praca Manoel Terra 330, Centro, 38025-015 Uberaba, MG, Brazil.

The aim of the present study was to assess the effects of an anticholinesterase agent, pyridostigmine bromide (Pyrido), on experimental chronic Chagas heart disease in mice. To this end, male C57BL/6J mice noninfected (control:Con) or chronically infected (5 months) with Trypanosoma cruzi (chagasic:Chg) were treated or not (NT) with Pyrido for one month. At the end of this period, electrocardiogram (ECG); cardiac autonomic function; heart histopathology; serum cytokines; and the presence of blood and tissue parasites by means of immunohistochemistry and PCR were assessed. In NT-Chg mice, significant changes in the electrocardiographic, autonomic, and cardiac histopathological profiles were observed confirming a chronic inflammatory response. Treatment with Pyrido in Chagasic mice caused a significant reduction of myocardial inflammatory infiltration, fibrosis, and hypertrophy, which was accompanied by a decrease in serum levels of IFNγ with no change in IL-10 levels, suggesting a shift of immune response toward an anti-inflammatory profile. Lower nondifferent numbers of parasite DNA copies were observed in both treated and nontreated chagasic mice. In conclusion, our findings confirm the marked neuroimmunomodulatory role played by the parasympathetic autonomic nervous system in the evolution of the inflammatory-immune response to T. cruzi during experimental chronic Chagas heart disease in mice.
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http://dx.doi.org/10.1155/2014/475946DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4158292PMC
May 2015

The importance of C4d in biopsies of kidney transplant recipients.

Clin Dev Immunol 2013 9;2013:678180. Epub 2013 Jul 9.

Discipline of General Pathology, Nephropathology Service, Federal University of Triângulo Mineiro, Frei Paulino, 30, 38025-180 Uberaba, Brazil.

Antibody-mediated rejection (AMR) is highly detrimental to the prolonged survival of transplanted kidneys. C4d has been regarded as a footprint of AMR tissue damage, and the introduction of C4d staining in daily clinical practice aroused an ever-increasing interest in the role of antibody-mediated mechanisms in allograft rejection. Despite the general acceptance of the usefulness of C4d in the identification of acute AMR, the data for C4d staining in chronic AMR is variable. The presence of C4d in the majority of the biopsies with features of chronic antibody-mediated rejection is reported, but this rejection without C4d staining is observed as well, suggesting that C4d is specific but not sensitive. Further studies on AMR with positive C4d staining in biopsy specimens are really important, as well as the study of novel routine markers that may participate in the pathogenesis of this process.
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http://dx.doi.org/10.1155/2013/678180DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3722852PMC
February 2014

Influence of gender on cardiac and encephalic inflammation in the elderly with cysticercosis: a case control study.

J Trop Med 2012 26;2012:540858. Epub 2012 Sep 26.

Biological Sciences Department, General Pathology Discipline, Triângulo Mineiro Federal University, Disciplina de Patologia Geral, Rua Frei Paulino no. 30, Bairro Abadia CEP: 38025-180 Uberaba, MG, Brazil.

Background. The present study explores the influence of the host's age and gender upon the inflammatory infiltrate. We aimed to quantify the inflammatory infiltrate caused by cysticercosis, which is related to aging, in the heart and in the encephalon. Methods. 75 autopsy protocols with cysticercosis diagnosis from department of pathology at a university hospital from 1970 to 2008 were reviewed. Two groups were formed: elderly with cysticercosis and nonelderly with cysticercosis. We used KS-300 (Kontron-Zeiss) software for morphometric analysis of the inflammation. Results. The elderly had an average of 3.1 ± 2.5 cysticerci, whereas the non-elderly had 2.7 ± 3.8 parasites. The non-elderly group with cysticercosis had significantly more inflammation, both cardiac and encephalic, than the elderly group. The elderly females with cysticercosis had more cardiac and encephalic inflammation. Conclusions. In this study, we showed that the non-elderly had significantly more cardiac and encephalic inflammation than the elderly, and that such inflammatory infiltrate decreases with age and depends upon the evolutionary stage of the cysticercus. Furthermore, there are differences concerning gender in the intensity of the inflammatory response due to cysticerci in the heart and brain parenchyma during senescence. Even during this period, women continue to have a more intense response to the parasitosis.
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http://dx.doi.org/10.1155/2012/540858DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3463955PMC
October 2012

Distinct expression of interleukin 17, tumor necrosis factor α, transforming growth factor β, and forkhead box P3 in acute rejection after kidney transplantation.

Ann Diagn Pathol 2013 Feb 8;17(1):75-9. Epub 2012 Sep 8.

Laboratory of Immunology, Federal University of Triângulo Mineiro, Uberaba, Minas Gerais, Brasil.

The kidney transplant is the main therapeutic alternative for end-stage kidney disease, and rejection is a major complication. The expression of proinflammatory cytokines is related to graft loss, whereas anti-inflammatory cytokines are associated with graft protection. The objective of this study is to evaluate the "in situ" expression of cytokines T helper 1 (tumor necrosis factor α [TNF-α]), T helper 17 (interleukin 17 [IL-17]), and regulatory T cell (transforming growth factor β [TGF-β]) and the expression of forkhead box P3 (FoxP3) in allograft kidney. We evaluated in situ expression of cytokines in allograft kidney under rejection process by indirect immunohistochemistry. Eighteen renal graft biopsies were from patients with episodes of rejection. The in situ expression of IL-17, TNF-α, and TGF-β was significantly higher in patients with acute rejection when compared with the control group. In contrast, analysis of FoxP3 expression showed few positive cells in patients with acute rejection compared with the control group. The results suggest that the expression of proinflammatory cytokines (IL-17 and TNF-α) contributes to the mechanisms of kidney transplant rejection. The increase in TGF-β expression might be an attempt to establish a process of immunoregulation or even to induce higher production of IL-17. The last hypothesis is supported by the observation of a reduced expression of FoxP3 and elevated levels of IL-17.
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http://dx.doi.org/10.1016/j.anndiagpath.2012.08.002DOI Listing
February 2013

[IgA nephropathy: histological analysis and clinicomorfological correlation in patients from Minas Gerais State].

J Bras Nefrol 2012 Jun;34(2):101-8

Universidade Federal do Triângulo Mineiro, Uberaba, MG, Brazil.

Introduction: IgA nephropathy (IgAN) is the most common primary glomerulopathy.

Objective: Classify IgAN according to the new Oxford's classification.

Methods: We analyzed the renal biopsies from the Nephropathology Service of UFTM, among 1996 to 2010, with a diagnosis of IgAN. We assessed gender, age, presence of hematuria, patterns/intensity of the lesions, deposition of IgA, IgG, IgM, Kappa, Lambda, C3, C1q and fibrinogen. Based on the histological alterations, the biopsies were characterized according to the Oxford Classification, and the clinicomorfological correlation was made. Significative results for p < 0,05.

Results: A total of 164 cases biopsies, predominantly male (53.7%) and adults (93.3%). We characterized the patients according Oxford Classification, there was a predominance of the pattern M0 (85,3%), S1 (53,1%), E0 (65,2%) e T0 (70,1%). About the clinicomorfological correlation, we observed more severe proteinuria comparing M1 to M0 (p < 0,008), low estimated GFR (p < 0,001) and more frequent hypertension (p < 0,001) comparing T0, T1 e T2. On immunofluorescence, there is a predominance of IgA (100% of cases), with co-deposition of C3 (99.37% of cases), Kappa (96.25%), Lambda (91.25%) and IgM (76.92%). Correlation was found between IgA intensity and C3, Kappa and Lambda.

Conclusion: In this study, IgA nephropathy was predominant in males, the more frequent patterns were the M0, S1, E0 and T0, with more severe proteinuria and the enhance of mesangial hypercellularity, besides larger prevalence of hypertension/worse kidney function according the tubulo-interstitial injuries.
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http://dx.doi.org/10.1590/s0101-28002012000200001DOI Listing
June 2012

Exercise-training reduced blood pressure and improve placental vascularization in pregnant spontaneously hypertensive rats--pilot study.

Fetal Pediatr Pathol 2012 Dec 16;31(6):423-31. Epub 2012 Apr 16.

Institute of Biological and Natural Sciences, Federal University of the Triângulo Mineiro (UFTM) , Uberaba , Brazil.

Assess the effects of exercise-training on resting arterial pressure and heart rate, placental fetuses morphologic alterations in pregnant spontaneously hypertensive rats (SHRs).Twenty SHRs and their respective control normotensive rats (WKY) were submitted or not to a swimming protocol during 9 weeks, resulting in four pregnant experimental groups: sedentary hypertensive (PSH), trained hypertensive (PTH), sedentary normotensive (PSN), and trained normotensive (PTN). Exercise-training by swimming attenuates arterial pressure in pregnant SHRs, and can contribute to an increase in the length of fetuses and the percentage of the vessels in the placenta.
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http://dx.doi.org/10.3109/15513815.2012.659535DOI Listing
December 2012

The relationship between heart rate variability and serum cytokines in chronic chagasic patients with persistent parasitemia.

Pacing Clin Electrophysiol 2011 Jun 28;34(6):724-35. Epub 2011 Jan 28.

Internal Medicine Department, Infectious Division, Federal University of the Triângulo Mineiro, Uberaba, Minas Gerais, Brazil.

Background: Persistent parasitemia, immunological, and autonomic nervous system impairments may play an important role in the evolution and clinical outcome of the chronic phase of Chagas' disease by triggering functional cardiovascular changes.

Methods: Three groups were evaluated: 17 chronic chagasic patients with the indeterminate form (IChD), 12 chronic chagasic patients with cardiac forms (ChHD), and 29 individuals as a healthy control group. Parasitemia was assessed by polymerase chain reaction; hemoculture, heart rate variability by linear and nonlinear methods, and interleukin (IL)-1β, IL-4, IL-6, IL-10, IL-12, IL-13, IL-17, and tumor necrosis factor-α, and interferon (IFN)-γ serum cytokines were assessed by enzyme-linked immune assay.

Results: Twenty-nine chronic chagasic patients were positive for parasitemia (17 IChD and 12 ChHD). Heart rate variability parameters in baseline condition and after cold face test were significantly decreased in chagasic patients compared to controls. Tilt tests showed no alteration. However, using nonlinear indices, ChHD patients presented lower values compared to IChD and controls. Differences in the expression of serum cytokines were observed between chagasic patients and controls. However, among the groups, ChHD presented higher median values of IL-10 and lower of IFN-γ compared to IChD.

Conclusion: Both chagasic groups present an autonomic impairment using linear methods. The nonlinear methods revealed that the ChHD group had a higher cardiovascular risk. Serum cytokine concentrations between chagasic patients were similar. However, ChHD showed higher concentrations of IL-10 and lower of IFN-γ, suggesting some established process of immune regulation.
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http://dx.doi.org/10.1111/j.1540-8159.2010.03025.xDOI Listing
June 2011

Acute Chagas' disease in postrenal transplant and treatment with benzonidazole.

Ann Diagn Pathol 2010 Jun 31;14(3):199-203. Epub 2009 Oct 31.

Discipline of General Pathology, Triângulo Mineiro Federal University, Uberaba, the State of Minas Gerais, Brazil.

Transplanted organs may act as a route of transmission of infectious diseases, such as Chagas' disease. The aim of this study was to describe the transmission of the Trypanosoma cruzi through a renal transplant and the anatomo-clinical evolution of the patient after treatment with benzonidazole. The patient was a 31-year-old white male from the State of Minas Gerais in Brazil. He had renal failure secondary to diabetes and later received a kidney from a cadaveric donor. The patient was undergoing immunosuppression therapy with azathioprine, cyclosporine A, and prednisone. After the transplant, he developed an acute phase of Chagas' disease and complications from diabetes and died 2 months later. In the autopsy, T cruzi amastigotes were found in the transplanted kidney, heart, bladder, liver, and pancreas. An important reduction in the parasitemia was obtained through the treatment of the infection with benzonidazole; however, the patient died due to complications from diabetes associated with tissue lesions caused by T cruzi.
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http://dx.doi.org/10.1016/j.anndiagpath.2009.06.008DOI Listing
June 2010

Diphyllobothrium latum infection in a non-endemic country: case report.

Rev Soc Bras Med Trop 2008 May-Jun;41(3):301-3

Núcleo de Medicina Tropical, Universidade de Brasília, Brasília, DF, Brazil.

Diphyllobothriasis, which is rarely described in Brazil, was reported initially as a travelers disease and as an accidental infection in individuals who ate raw freshwater fish. This report aims to present the case of a 20-year-old patient with confirmed Diphyllobothrium latum infection.
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http://dx.doi.org/10.1590/s0037-86822008000300015DOI Listing
January 2009

Cysticercosis in the elderly.

Ann Diagn Pathol 2007 Oct;11(5):330-3

Biomedical, Graduated Course in Pathology, Triangulo Mineiro Federal University, Uberaba, Minas Gerais, Brazil.

The immunosenescence, a peculiarity of the aging process, increases the susceptibility of elderly individuals to neoplasia, infections, and parasitosis, among them, cysticercosis. The aim of this study was to compare the epidemiological and anatomopathological characteristics of cysticercosis in elderly or nonelderly patients. We reviewed 72 autopsies, protocols and specimens, performed in the School Hospital of the Triangulo Mineiro Federal University. We selected 3 groups: elderly with cysticercosis, nonelderly with cysticercosis, and control group without cysticercosis. From the patients with cysticercosis, 27.8% were elderly. Of these, 80% presented with neurocysticercosis and 20% cardiac cysticercosis. In the elderly, the early stages of the parasitic development, vesicular and colloidal vesicular, prevailed (75%). In relationship with the causes of death, death in 40% of the elderly was by neoplasic and 35% by cardiovascular, whereas for the nonelderly, death in 63.5% was by cardiovascular and 25% by infectious. There was a significant difference between groups with relation to neoplasic and cardiovascular causes. Therefore, cysticercosis was frequent in elderly patients, and probably patients are continuously infected with cysticercosis as they age. Perhaps, decurrence of the immunosenescence associated with immunological alterations caused by cysticercosis form favorable conditions to the development of neoplasias in the elderly attacked by the parasitosis.
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http://dx.doi.org/10.1016/j.anndiagpath.2006.12.008DOI Listing
October 2007

Anatomopathological aspects of neurocysticercosis in autopsied patients.

Arq Neuropsiquiatr 2007 Mar;65(1):87-91

General Pathology Disciplines, Federal University of Triangulo Mineiro, Uberabe, Minas Gerais, Brazil.

The aim of this paper was to describe the occurrence and morphology of neurocysticercosis (NCC) in autopsies. We revised 2218 autopsies performed at the School Hospital from Federal University of Triangulo Mineiro, 1970-2003. Data referring to age, gender and color of patients were reported and NCC was microscopically and macroscopically analyzed. We found 53 (2.4%) NCC cases. The mean age was 50 years old, 34 (64.1%) individuals were male and 36 (67.9%) white. Macroscopically, 17 cysticerci were analyzed. The most frequent location was meningocortical in 12 (70.6%) cases. Microscopically, the cysticerci presented an ovoid shape, containing the larvae preserved in 4 (23.5%) cases or in destruction degrees in 13 (76.5%) cases. Therefore, in NCC was found several general pathologic processes (necrosis, interstitial deposits, fibrosis, gliosis, inflammation) amongst which are highlighted beta-fibrillose in 13 (76.5%) cases associated to inflammatory process in 16 (94.1%) cases caused by the parasite, not yet related to NCC, and calcification present in viable and destruction parasites.
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http://dx.doi.org/10.1590/s0004-282x2007000100019DOI Listing
March 2007