Publications by authors named "Ana Baptista"

37 Publications

Electronic control of drug release from gauze or cellulose acetate fibres for dermal applications.

J Mater Chem B 2021 04;9(16):3515-3522

CENIMAT/I3N, Departamento de Ciência dos Materiais, Faculdade de Ciências e Tecnologia, FCT, Universidade NOVA de Lisboa, 2829-516 Campus de Caparica, Portugal.

Electronic controlled drug release from fibres was studied using ibuprofen as a model drug, one of the most popular analgesics, to impregnate gauze and cellulose acetate (CA) membranes. Conductivity in the range of 1-10 mS cm-1 was obtained in polypyrrole (Ppy) functionalised gauze and CA fibres, providing voltage-controlled drug release in a system consisting of Ppy/Ibuprofen/Ppy membranes and an Ag electrode. SEM images evidenced the Ppy adhesion to fibres and Micro Raman spectra proved drug incorporation and release. A small wound adhesive built with these membranes retains ibuprofen at 1.5 V and quickly releases it when -0.5 V is applied.
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http://dx.doi.org/10.1039/d1tb00249jDOI Listing
April 2021

Hypoglycemic episodes in hospitalized people with diabetes in Portugal: the HIPOS-WARD study.

Clin Diabetes Endocrinol 2021 Jan 5;7(1). Epub 2021 Jan 5.

MSD Portugal, R. Qta da Fonte 19, 2770-192, Paço de Arcos, Portugal.

Background: We intended to estimate the proportion hypoglycemic/hyperglycemic emergency episodes in treated diabetes mellitus (DM) patients admitted to a hospital ward, and calculate the prevalence of risk factors for hypoglycemia and diabetic complications.

Methods: In this cross-sectional, multicentered study, the observational data was collected by physicians from patient's hospitalization to discharge/death. Statistical tests were 2-tailed considering 5% significance level.

Results: There were 646 ward admissions due to hyperglycemic emergencies and 176 hypoglycemic episodes with a ratio hypoglycemia/hyperglycemia 0.27 for all DM patients. In T2DM patients the ratio was 0.38. These were mainly female (55.1%), functionally dependent (61.4%) and retired/disabled (73.1%). Median age was 75 years and median duration of disease 11 years. Half the patients were on insulin-based therapy and 30.1% on secretagogue-based therapy. Approximately 57% of patients needed occasional/full assistance to manage the disease. The most frequent risk factor for hypoglycemia was polypharmacy (85.0%). Hypoglycemia in the 12 months before admission was higher in insulin-based therapy patients (66.1%; p = 0.001).

Conclusions: Hyperglycemic emergencies are the most frequent cause of hospitalization in Portugal, although severe hypoglycemic events represent a health and social problem in elderly/frail patients. There is still the need to optimize therapy in terms of the potential for hypoglycemia in this patient group and a review of anti-hyperglycemic agents to add on to insulin.
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http://dx.doi.org/10.1186/s40842-020-00114-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7786902PMC
January 2021

Tryptophan Attenuates the Effects of OTA on Intestinal Morphology and Local IgA/IgY Production in Broiler Chicks.

Toxins (Basel) 2020 Dec 23;13(1). Epub 2020 Dec 23.

Department of Pathological Sciences, State University of Londrina, P.O. Box 10.011, Londrina, PR 86057-970, Brazil.

Ochratoxin A (OTA) is a mycotoxin produced by species of and that can contaminate products of plant origin that are used as animal feed. Through oral exposure, this mycotoxin primarily affects the chicken gastrointestinal system. The present study evaluated the intestinal toxic effects of OTA and the introduction of L-tryptophan to alleviate these effects in chickens. One-day-old chicks were exposed to a single OTA dose (1.4 mg/kg body weight-b.w.) and treated with or without four daily doses of L-tryptophan (100 mg/kg b.w.). Duodenal villus height/crypt depth, fecal immunoglobulin A/immunoglobulin Y (IgA/IgY) levels, and duodenal positive immunoglobulin A cells (IgA) were evaluated by histology, ELISA, and immunohistochemistry, respectively, on the 14th day. There were significant changes in the duodenal villus height, crypt depth, and levels of fecal IgA/IgY and duodenal IgA cells ( < 0.05) in groups exposed to OTA. On the other hand, groups exposed to OTA and treated with L-tryptophan showed similar levels of villus height, IgA/IgY levels, and duodenal IgA cells to those of the control group ( > 0.05). In conclusion, exposure to a single dose of OTA orally induces changes in intestinal morphology, levels of IgA/IgY antibodies, and IgA cells. Thus, treatment with L-tryptophan may be a valid alternative means to reduce the harmful effects of OTA on the intestinal mucosa, which requires further study.
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http://dx.doi.org/10.3390/toxins13010005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823272PMC
December 2020

Can children's oral hygiene and sleep routines be compromised during the COVID-19 pandemic?

Int J Paediatr Dent 2021 Jan 13;31(1):12-19. Epub 2020 Oct 13.

Department of Pediatric Destistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.

Background: During COVID-19 pandemic, children are confined at home, with changes in family routines.

Aim: Evaluate sleep disorders among Brazilian and Portuguese children during social distancing, and its association with parental perception of child's oral hygiene.

Design: In this cross-sectional study, Portuguese and Brazilian parents/caregivers of 3- to 15-year-old children, practicing social distancing due to COVID-19 pandemic, answered an online questionnaire, from April 24-26, 2020, evaluating sociodemographic characteristics, child's school activities online, child's sleep quality during social distancing. Two questions from the questionnaire, developed based on previous studies, evaluated the parental perception of child's oral hygiene quality and routine changes during social distancing. Parents/caregivers answered five domains of the Portuguese-language version of the Sleep Disturbances Scale for Children, evaluating sleep-breathing disorders, disorders of arousal, sleep-wake transition disorders, disorders of excessive somnolence and sleep hyperhidrosis. Descriptive, Linear-by-linear association, Kruskal-Wallis and post hoc analysis were performed (P ≤ .05).

Results: Participated in the study 253 parents/caregivers, 50.2% from Brazil. Most parents (72.2%) reported changes in child's routine during social distancing. Sleep breathing disorders (P = .019), sleep-wake transition disorders (P = .022), and disorders of excessive somnolence (P < .001) were associated with poor oral hygiene during social distancing.

Conclusion: Sleep disorders are associated with poor oral hygiene during social distancing.
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http://dx.doi.org/10.1111/ipd.12732DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7537505PMC
January 2021

Lactobacillus spp. reduces morphological changes and oxidative stress induced by deoxynivalenol on the intestine and liver of broilers.

Toxicon 2020 Oct 18;185:203-212. Epub 2020 Jul 18.

Laboratory of Animal Pathology, Universidade Estadual de Londrina, Campus Universitário, Rodovia Celso Garcia Cid, Km 380, Londrina, Paraná, 86057-970, Brazil. Electronic address:

The mycotoxin deoxynivalenol (DON) contaminates animal feed worldwide, frequently resulting in poor performance and economic losses. Data concerning the effects on poultry health or focusing on intestinal toxicity or the response to oxidative stress are scarce. Also, there is a need for strategies to mitigate the negative effects of DON. This study aimed to investigate the effects of Lactobacillus spp. treatments on the intestine, liver and kidney of poultry fed a DON-contaminated diet. To achieve this aim, histological, morphometrical and histochemical assays were performed. The oxidative stress response was also analyzed by the tests: reduced glutathione, ferric reducing ability, reducing of 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid), nitro blue tetrazolium detection of superoxide anion, and thiobarbituric acid reactive substances. One-day-old broilers chickens (n 50) were submitted to the following treatments: control, DON (19.3 mg kg), viable Lactobacillus spp. + DON (VL + DON), heat-inactivated Lactobacillus spp. + DON (HIL + DON), Lactobacillus spp. culture supernatant + DON (LCS + DON). The animals received the contaminated diet for seven days. DON increased the intestinal and liver lesion score, while the Lactobacillus spp. treatments (LT) remained like the control. DON reduced the villi height and increased the crypt depths. The LT showed crypt depths similar to control, and higher villi: crypt ratio in duodenum and jejunum. In the ileum, the LT reduced the goblet cell count in relation to DON group. DON increased the number of intraepithelial lymphocytes (IEL) in jejunum and ileum, while the VL + DON treatment induced a significant decrease in IEL in comparison to DON. DON-diet induced an oxidative stress response in the intestine and liver, and also reduced the antioxidant capacity in these tissues, while LT treatments remained mostly similar to control. DON induced no change in redox balance in the kidney. The LT improved the intestinal health after DON acute exposure, reducing the oxidative stress damage mainly on jejunum and liver.
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http://dx.doi.org/10.1016/j.toxicon.2020.07.002DOI Listing
October 2020

Hospitalization Costs Due to Hypoglycemia in Patients with Diabetes: A Microcosting Approach.

Diabetes Ther 2020 Oct 11;11(10):2237-2255. Epub 2020 Jul 11.

MSD Portugal, Paço de Arcos, Portugal.

Introduction: Hypoglycemia leading to hospitalization is associated with adverse economic outcomes, although the real burden is unknown. The HIPOS-WARD (Hypoglycemia In Portugal Observational Study-Ward) aimed to characterize ward admissions due to hypoglycemia episodes in treated patients with diabetes and assess their economic impact to the National Health System.

Methods: Observational, cross-sectional study, conducted in 16 Portuguese centers for 22 months. The applied microcosting approach was based on healthcare resource data, collected from patients' charts upon ward admission until discharge, and unitary costs from official/public data sources. Absenteeism was also estimated for active workers on the basis of the human capital approach.

Results: Of the 176 patients with diabetes mellitus enrolled, 86% had type 2 diabetes. Half of the patients (50.0%) were on insulin-based therapy, followed by 30.1% on a secretagogue-based regimen, 9.7% on non-secretagogue therapy, and 10.2% on a combination of insulin and secretagogue. Overall mean costs per patient were medication, 45.45 €; laboratory analysis, 218.14 €; examinations, 64.91 €; physician and nurse time, 268.55 € and 673.39 €, respectively. Bed occupancy was the main cost driver (772.09 €) and indirect cost averaged 140.44 €. Overall, the cost per hypoglycemia episode leading to hospitalization averaged 2042.52 € (range 194.76-16,762.87 €). Patients treated with insulin-based regimens (2267.76 €) and type 2 diabetes (2051.29 €) had the highest mean costs. The mean cost increased with repeated hypoglycemic events (2191.67 €), correlated complications (2109.26 €), and death (5253.38 €).

Conclusion: HIPOS-WARD's findings confirm and support both the substantial clinical and economic impact of hospitalization due to hypoglycemia in Portugal.
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http://dx.doi.org/10.1007/s13300-020-00868-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7509029PMC
October 2020

Carbon threads sweat-based supercapacitors for electronic textiles.

Sci Rep 2020 May 7;10(1):7703. Epub 2020 May 7.

CENIMAT/i3N, Departamento de Ciência dos Materiais, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, 2829-516, Portugal.

Flexible and stretchable energy-storage batteries and supercapacitors suitable for wearable electronics are at the forefront of the emerging field of intelligent textiles. In this context, the work here presented reports on the development of a symmetrical wire-based supercapacitor able to use the wearer's sweat as the electrolyte. The inner and outer electrodes consists of a carbon-based thread functionalized with a conductive polymer (polypyrrole) which improves the electrochemical performances of the supercapacitor. The inner electrode is coated with electrospun cellulose acetate fibres, as the separator, and the outer electrode is twisted around it. The electrochemical performances of carbon-based supercapacitors were analyzed using a simulated sweat solution and displayed a specific capacitance of 2.3 F.g, an energy of 386.5 mWh.kg and a power density of 46.4 kW.kg. Moreover, cycle stability and bendability studies were performed. Such energy conversion device has exhibited a stable electrochemical performance under mechanical deformation, over than 1000 cycles, which make it attractive for wearable electronics. Finally, four devices were tested by combining two supercapacitors in series with two in parallel demonstrating the ability to power a LED.
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http://dx.doi.org/10.1038/s41598-020-64649-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7206100PMC
May 2020

Proteus mirabilis causing cellulitis in broiler chickens.

Braz J Microbiol 2020 Sep 17;51(3):1353-1362. Epub 2020 Feb 17.

Department of Microbiology, Laboratory of Bacteriology, Center of Biological Sciences, State University of Londrina, Rodovia Celso Garcia Cid PO-BOX 6001, Londrina, Paraná, 86051-980, Brazil.

Given the need to understand the virulence profile of Proteus mirabilis isolates from cellulitis in broiler chickens and their ability to cause lesions, the present study aimed to characterize genotypically and phenotypically the virulence profiles of two strains of P. mirabilis isolated from cellulitis in broilers, as well as to evaluate their ability to experimentally reproduce the lesions in vivo. The strain with the highest virulence potential (LBUEL-A33) possessed mrpA, pmfA, ucaA, atfA (fimbriae), zapA, ptA (proteases), hpmA (hemolysin), and ireA (siderophore) genes, formed a very strong biofilm, and expressed the pattern of aggregative adhesion and cytotoxicity in Vero cells. The strain with the lowest virulence potential (LBUEL-A34) did not present the pmfA and ucaA genes, but expressed the pattern of aggregative adhesion, formed a strong biofilm, and did not show cytotoxicity. Both strains developed cellulitis in an animal model within 24 h post-inoculation (PI), and the degree of lesions was not significantly altered up to 120 h PI. The LBUEL-A33 strain was also inoculated in combination with an avian pathogenic Escherichia coli (APEC 046), and the lesions showed no significant changes from the individual inoculation of these two strains. Histological analysis showed that the LBUEL-A33 strain developed characteristic cellulitis lesions. Thus, both strains of P. mirabilis isolated in our study have several virulence factors and the ability to develop cellulitis in broilers.
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http://dx.doi.org/10.1007/s42770-020-00240-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7455630PMC
September 2020

[Severe Thrombocitopenia Induced by Fenofibrate].

Acta Med Port 2020 Jan 3;33(1):58-61. Epub 2020 Jan 3.

Serviço de Medicina Interna. Centro Hospitalar Universitário do Algarve. Faro. Portugal.

Drug-induced thrombocytopenia is a common entity in clinical practice. However, having in consideration the severity of the case, it becomes imperative to distinguish non-immune thrombocytopenia from the po-tentially life-threatening immune-mediated forms. The authors report a rare clinical case of a 79-year-old man presenting with purpuric rash and gingival hemorrhage while on fenofibrate treatment (sixth day). The evolu-tion was favorable after drug removal and corticosteroid administration. Drug-associated thrombocytopenia is reported by manufacturers as an extremely rare event. This is the second case report of immune throm-bocytopenia to fenofibrate. The first event was reported for publication in 2015.
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http://dx.doi.org/10.20344/amp.11354DOI Listing
January 2020

Customized tracheal design using 3D printing of a polymer hydrogel: influence of UV laser cross-linking on mechanical properties.

3D Print Med 2019 Aug 2;5(1):12. Epub 2019 Aug 2.

i3N/CENIMAT, Department of Materials Science, Lisbon, Portugal.

Background: The use of 3D printing of hydrogels as a cell support in bio-printing of cartilage, organs and tissue has attracted much research interest. For cartilage applications, hydrogels as soft materials must show some degree of rigidity, which can be achieved by photo- or chemical polymerization. In this work, we combined chemical and UV laser polymeric cross-linkage to control the mechanical properties of 3D printed hydrogel blends. Since there are few studies on UV laser cross-linking combined with 3D printing of hydrogels, the work here reported offered many challenges.

Methods: Polyethylene glycol diacrylate (PEGDA), sodium alginate (SA) and calcium sulphate (CaSO) polymer paste containing riboflavin (vitamin B2) and triethanolamine (TEOHA) as a biocompatible photoinitiator was printed in an extrusion 3D plotter using a coupled UV laser. The influence of the laser power on the mechanical properties of the printed samples was then examined in unconfined compression stress-strain tests of 1 × 1 × 1 cm sized samples. To evaluate the adhesion of the material between printed layers, compression measurements were performed along the parallel and perpendicular directions to the printing lines.

Results: At a laser density of 70 mW/cm, Young's modulus was approximately 6 MPa up to a maximum compression of 20% in the elastic regime for both the parallel and perpendicular measurements. These values were within the range of biological cartilage values. Cytotoxicity tests performed with Vero cells confirmed the cytocompatibility.

Conclusions: We printed a partial tracheal model using optimized printing conditions and proved that the materials and methods developed may be useful for printing of organ models to support surgery or even to produce customized tracheal implants, after further optimization.
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http://dx.doi.org/10.1186/s41205-019-0049-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6743139PMC
August 2019

Genotypic and phenotypic profiles of virulence factors and antimicrobial resistance of Proteus mirabilis isolated from chicken carcasses: potential zoonotic risk.

Braz J Microbiol 2019 Jul 3;50(3):685-694. Epub 2019 May 3.

Laboratory of Bacteriology, Department of Microbiology, Center of Biological Sciences, Universidade Estadual de Londrina, Londrina, Brazil.

Proteus mirabilis is an opportunistic pathogen often associated with a variety of human infections acquired both in the community and in hospitals. In this context, the present work aimed to evaluate the genotypic and phenotypic characteristics of the virulence factors and antimicrobial resistance determinants of 32 P. mirabilis strains isolated from chicken carcasses in a poultry slaughterhouse in the north of the state of Paraná, Brazil, in order to assess a potential zoonotic risk. The isolates presented a variety of virulence genes that contribute to the development of infection in humans. The mrpA, pmfA, atfA (fimbriae), ireA (siderophores receptor), zapA, ptA (Proteases), and hpmA (hemolysin) genes were found in 32 (100%) isolates and ucaA (fimbriae) in 16 (50%). All isolates showed aggregative adherence in HEp-2 cells and formed biofilms. Of all strains, 27 (84.38%) showed cytotoxic effects in Vero cells. Antimicrobial susceptibility was tested using 20 antimicrobials, in which 25 (78.13%) strains were considered multidrug-resistant. The presence of bla and bla genes conferring resistance to β-lactams and qnr to quinolones were also detected in the isolates after presumption in the phenotypic test, in which 7 (21.88%) isolates contained the CTX-M-2 group, 11 (34.38%) contained CIT group and 19 (59.38%) contained qnrD. Therefore, chicken carcasses contaminated with P. mirabilis may pose a health risk to the consumer, as these isolates have a variety of virulence and antimicrobial resistance characteristics that can be found in P. mirabilis strains isolated from human infections.
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http://dx.doi.org/10.1007/s42770-019-00086-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6863274PMC
July 2019

Faculty as Active Learners About Their Practice: Toward Innovation and Change in Nursing Education.

J Contin Educ Nurs 2019 Mar;50(3):134-140

This article describes the perceptions of a small group of nursing education faculty members participating in the continuing professional development module, Pedagogy in Higher Education, regarding the use of active learning strategies. Data were collected using field notes and participants' presentations and articles about their experiences when using innovative teaching strategies. The findings point out the benefits for knowledge mobilization, the development of synergies based on collaboration, and other gains in terms of students' experiences. Challenges involve the use of these active strategies on a regular basis as part of a sustained and aligned approach to enhance teaching and learning. [J Contin Educ Nurs. 2019;50(3):134-140.].
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http://dx.doi.org/10.3928/00220124-20190218-09DOI Listing
March 2019

A pruritic vesicular rash.

Aust Fam Physician 2017 Jan/Feb;46(1):45-47

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August 2017

Widespread rash after throat infection.

Aust Fam Physician 2016 Aug;45(8):579-80

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August 2016

Screening for Fabry disease in left ventricular hypertrophy: documentation of a novel mutation.

Arq Bras Cardiol 2015 Aug 7;105(2):139-44. Epub 2015 Aug 7.

Unidade de Vila Real, Centro Hospitalar de Trás-os-Montes e Alto Douro, PT.

Background: Fabry disease is a lysosomal storage disease caused by enzyme α-galactosidase A deficiency as a result of mutations in the GLA gene. Cardiac involvement is characterized by progressive left ventricular hypertrophy.

Objective: To estimate the prevalence of Fabry disease in a population with left ventricular hypertrophy.

Methods: The patients were assessed for the presence of left ventricular hypertrophy defined as a left ventricular mass index ≥ 96 g/m2 for women or ≥ 116 g/m2 for men. Severe aortic stenosis and arterial hypertension with mild left ventricular hypertrophy were exclusion criteria. All patients included were assessed for enzyme α-galactosidase A activity using dry spot testing. Genetic study was performed whenever the enzyme activity was decreased.

Results: A total of 47 patients with a mean left ventricular mass index of 141.1 g/m2 (± 28.5; 99.2 to 228.5 g/m2] were included. Most of the patients were females (51.1%). Nine (19.1%) showed decreased α-galactosidase A activity, but only one positive genetic test - [GLA] c.785G>T; p.W262L (exon 5), a mutation not previously described in the literature. This clinical investigation was able to establish the association between the mutation and the clinical presentation.

Conclusion: In a population of patients with left ventricular hypertrophy, we documented a Fabry disease prevalence of 2.1%. This novel case was defined in the sequence of a mutation of unknown meaning in the GLA gene with further pathogenicity study. Thus, this study permitted the definition of a novel causal mutation for Fabry disease - [GLA] c.785G>T; p.W262L (exon 5).
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http://dx.doi.org/10.5935/abc.20150090DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4559122PMC
August 2015

Fluency profile: comparison between Brazilian and European Portuguese speakers.

Codas 2014 Nov-Dec;26(6):444-6. Epub 2014 Dec 1.

Speech Language Pathology and Audiology Course, Universidade de Brasília, Brasília, DF, Brazil.

The purpose of the study was to compare the speech fluency of Brazilian Portuguese speakers with that of European Portuguese speakers. The study participants were 76 individuals of any ethnicity or skin color aged 18-29 years. Of the participants, 38 lived in Brazil and 38 in Portugal. Speech samples from all participants were obtained and analyzed according to the variables of typology and frequency of speech disruptions and speech rate. Descriptive and inferential statistical analyses were performed to assess the association between the fluency profile and linguistic variant variables. We found that the speech rate of European Portuguese speakers was higher than the speech rate of Brazilian Portuguese speakers in words per minute (p=0.004). The qualitative distribution of the typology of common dysfluencies (p<0.001) also discriminated between the linguistic variants. While a speech fluency profile of European Portuguese speakers is not available, speech therapists in Portugal can use the same speech fluency assessment as has been used in Brazil to establish a diagnosis of stuttering, especially in regard to typical and stuttering dysfluencies, with care taken when evaluating the speech rate.
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http://dx.doi.org/10.1590/2317-1782/20142014184DOI Listing
May 2015

Identification and adhesion profile of Lactobacillus spp. strains isolated from poultry.

Braz J Microbiol 2014 9;45(3):1065-73. Epub 2014 Oct 9.

Laboratório de Ornitopatologia Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista BotucatuSP Brazil Laboratório de Ornitopatologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Botucatu, SP, Brazil.

In the aviculture industry, the use of Lactobacillus spp. as a probiotic has been shown to be frequent and satisfactory, both in improving bird production indexes and in protecting intestine against colonization by pathogenic bacteria. Adhesion is an important characteristic in selecting Lactobacillus probiotic strains since it impedes its immediate elimination to enable its beneficial action in the host. This study aimed to isolate, identify and characterize the in vitro and in vivo adhesion of Lactobacillus strains isolated from birds. The Lactobacillus spp. was identified by PCR and sequencing and the strains and its adhesion evaluated in vitro via BMM cell matrix and in vivo by inoculation in one-day-old birds. Duodenum, jejunum, ileum and cecum were collected one, four, 12 and 24 h after inoculation. The findings demonstrate greater adhesion of strains in the cecum and an important correlation between in vitro and in vivo results. It was concluded that BMM utilization represents an important technique for triage of Lactobacillus for subsequent in vivo evaluation, which was shown to be efficient in identifying bacterial adhesion to the enteric tract.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4204948PMC
http://dx.doi.org/10.1590/s1517-83822014000300040DOI Listing
July 2015

[Fibroelastoma causing intermittent coronary obstruction and myocardial infarction: When the echocardiogram makes the difference].

Rev Port Cardiol 2014 Jun 4;33(6):395-6. Epub 2014 Jul 4.

Serviço de Cardiologia, Hospital de S. Pedro, CHTMAD, Vila Real, Portugal.

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http://dx.doi.org/10.1016/j.repc.2013.12.004DOI Listing
June 2014

[Mural thrombus and thoracic aortic aneurysm: An unusual association].

Rev Port Cardiol 2014 Jun 4;33(6):393-4. Epub 2014 Jul 4.

Serviço de Cardiologia, Centro Hospitalar de Trás-os-Montes e Alto Douro - Unidade de Vila Real, Vila Real, Portugal.

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http://dx.doi.org/10.1016/j.repc.2014.01.004DOI Listing
June 2014

Bacteriophage-induced reduction in Salmonella Enteritidis counts in the crop of broiler chickens undergoing preslaughter feed withdrawal.

Poult Sci 2014 Jan;93(1):216-20

São Paulo State University, Clinical Sciences, Botucatu, São Paulo, 18618-000 Brazil.

Salmonella food poisoning is a public health problem. Feed withdrawal from broiler chickens before slaughter can favor the multiplication of Salmonella in the cecum and crop of contaminated animals and subsequently lead to contamination of carcasses in the processing plant. In the present study, a cocktail of lytic bacteriophages isolated from sewage water was orally administered to 45-d-old broiler chickens 1 h after they received an oral dose of 10(7) cfu/mL Salmonella enterica subspecies enterica serotype Enteritidis. Immediately after phage administration and 30 min, 1, 3, 6, and 12 h thereafter, groups of chicken were killed. Ceca and crops were analyzed for the presence of Salmonella. At 3 h posttreatment, there were 10(3) cfu/g and 10(1) cfu/g of cecal and crop suspension, respectively. At 6 h after treatment, the number of Salmonella was 10(3) cfu/g in the cecal suspension, but below the detection limit in the crops. Our results suggest that bacteriophage therapy may be able to reduce the contamination of chicken carcasses by reducing the preslaughter load of Salmonella in the birds.
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http://dx.doi.org/10.3382/ps.2013-03360DOI Listing
January 2014

Influence of deceased donor hemodynamic factors in transplant recipients renal function.

J Bras Nefrol 2013 Oct-Dec;35(4):289-98

Introduction: The incidence of delayed graft function (DGF) and unsatisfactory creatinine clearance (UCC) after renal transplantation is significantly higher in Brazil, when compared with that observed in United States or Europe. Deceased donor (DD) characteristics should directly influence the occurrence of these two outcomes.

Objective: This study aim to evaluate the influence of DD characteristics on DGF and UCC incidence in Brazil.

Methods: DD clinical and laboratory variables were correlated with outcome's incidence.

Results: We evaluated 787 DD whose organs were transplanted in 1298 patients. We noted a high prevalence of vasoactive drugs use (90.2%), hypernatremia (66.6%) and renal dysfunction (34.8%). The incidence of DGF and UCC was 60.6% and 55.2%, respectively. We observed a progressive increase in DGF risk for age groups over 30 years and for cold ischemia time (CIT) greater than 24 hours. DGF risk was two times higher in recipients of donor kidney final serum creatinine (Cr) over than 1.5 mg/dl. Hypertension and CIT over 36 hours was associated with an increasing of 82% and 99% in UCC risk, respectively. Donor age above 40 years was associated with a progressive increase in UCC risk.

Conclusion: DD age, renal function, hypertension and prolonged CIT were associated with increased risk DGF and UCC.
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http://dx.doi.org/10.5935/0101-2800.20130048DOI Listing
April 2015

Antibodies attacking the brain: Is it time for a paradigm shift in psychiatric practice and service models?

Aust N Z J Psychiatry 2013 Dec 29;47(12):1108-12. Epub 2013 Oct 29.

1Department of Liaison Psychiatry, St George's Hospital, London, UK.

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http://dx.doi.org/10.1177/0004867413510053DOI Listing
December 2013

[Dysphagia lusoria - a clinical report].

Rev Port Cir Cardiotorac Vasc 2013 Jul-Sep;20(3):163-5

Serviço de Angiologia e Cirurgia Vascular do Centro Hospitalar e Universitário de Coimbra, Portugal.

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June 2015

Stent Thrombosis eight years past drug-eluting stent placement: a case report.

Arq Bras Cardiol 2013 May;100(5):e59-61

Centro Hospitalar de Trás-os-Montes e Alto Douro - Unidade de Vila Real.

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http://dx.doi.org/10.5935/abc.20130095DOI Listing
May 2013

[Pulmonary hypertension, heart failure and hyperthyroidism: a case report].

Rev Port Cardiol 2013 Mar 11;32(3):253-6. Epub 2013 Jan 11.

Serviço de Cardiologia, Centro Hospitalar de Trás-os-Montes e Alto Douro, Vila Real, Portugal.

We present a case of acute heart failure as the first manifestation of Graves' disease. It illustrates some of its cardiovascular complications, particularly atrial fibrillation, pulmonary hypertension and heart failure. This case report highlights the importance of considering hyperthyroidism as a cause of idiopathic pulmonary hypertension, and demonstrates the potential reversibility of its complications.
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http://dx.doi.org/10.1016/j.repc.2012.12.005DOI Listing
March 2013

Severe aneurysmal coronary artery disease in a patient with ulcerative colitis.

Rev Port Cardiol 2012 Jul-Aug;31(7-8):525-6. Epub 2012 Jun 14.

Serviço de Cardiologia, Centro Hospitalar de Trás-os-Montes-e-Alto-Douro, Vila Real, Portugal.

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http://dx.doi.org/10.1016/j.repc.2012.01.016DOI Listing
October 2012

[Pacemaker lead-induced tricuspid stenosis: a report of two cases].

Rev Port Cardiol 2012 Apr 20;31(4):305-8. Epub 2012 Mar 20.

Serviço de Cardiologia, Centro Hospitalar de Trás-os-Montes e Alto Douro, Unidade de Vila Real, Vila Real, Portugal.

Tricuspid stenosis (TS) is an uncommon complication of transvenous ventricular pacemaker implantation, with few cases reported in the literature. The mechanisms described are obstruction of right ventricular inflow by tricuspid vegetations (endocarditis), multiple pacemaker leads and tricuspid valve (TV) fibrosis secondary to perforation or laceration of the TV leaflets, or adherence between redundant portions of the lead and valvular and subvalvular tissue. We report two cases of severe TS, with different etiologies and management: one caused by leaflet perforation, resolved surgically, and the other secondary to fusion between a loop of the pacemaker lead and the subvalvular apparatus, which was treated medically.
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http://dx.doi.org/10.1016/j.repc.2012.02.008DOI Listing
April 2012

[Cardiac injury in the context of metastatic hepatocellular carcinoma].

Rev Port Cardiol 2011 Sep;30(9):731-4

Serviço de Cardiologia, Centro Hospitalar de Trás-os-Montes e Alto Douro, Hospital de S. Pedro, Vila Real, Portugal.

Hepatocellular carcinoma with extension or metastasis to the right atrium is an uncommon form of cardiac malignancy. The authors report the case of a 51-year-old patient with hepatocellular carcinoma and thrombi in the portal and mesenteric veins, which histopathology revealed to be metastatic. Echocardiography showed a right atrial mass which in this context has to be considered as a possible cardiac metastasis.
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http://dx.doi.org/10.1016/S0870-2551(11)70017-8DOI Listing
September 2011

[Ecodoppler evaluation on the progress of atherosclerotic disease in patients undergoing contralateral carotid endarterectomy].

Rev Port Cir Cardiotorac Vasc 2011 Apr-Jun;18(2):99-104

Serviço de Angiologia e Cirurgia Vascular do Hospital Clínico Universitário de Valladolid, Espanha.

Purpose: The clinical utility of monitoring by Doppler ultrasound patients undergoing carotid endarterectomy is not clearly established. Our goal is to evaluate by echo-Doppler the progress of contralateral stenosis in a real environment, in order to assess its usefulness.

Material And Methods: Retrospective study with Doppler ultrasound monitoring in patients undergoing carotid endarterectomy for a period of 3 years. We included cases with Doppler echography before and at least one post-surgery. We excluded patients with contralateral thrombosis, contralateral stenosis, established surgical indication or prior carotid surgery. Analysis was performed using Kaplan-Meier curves to estimate the rate of patients free of progression and / or surgery, as well as an analysis of risk factors for progression of contralateral stenosis.

Results: We included 119 patients (41.2% symptomatic) age 68.3 +/- 8.41 years. The follow-up was 40.5 +/- 23.2 months, with a mean of 2.64 +/- 1.58 scans per patient. The likelihood of progression of contralateral carotid stenosis was estimated at 91, 86, 81 and 79% at one, two, three and four years respectively. The only significant risk factor for progression was the presence of moderate or greater contralateral stenosis (30% or more) at the time of surgery.

Conclusions: The evolution of the contralateral carotid atherosclerosis is relatively common. The degree of stenosis correlates with early progression, the study by echo-Doppler plays an important role in monitoring these patients.
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June 2015

Necrotising granulomatous lymphadenitis.

BMJ Case Rep 2011 Feb 8;2011. Epub 2011 Feb 8.

Department of Internal Medicine, Faro's Hospital, Rua leao penedo, Faro, Portugal.

Extrapulmonary tuberculosis (EPTB) is defined as Mycobacterium TB through Ziehl-Neelsen acid-fast stain and culture in Loewenstein-Jensen in a tissue from a site other than lung parenchyma, in association with clinical or imaging findings compatible with infection locally. The authors report a case of a patient who presented with asthenia, anorexia and weight loss. He complained of fever, chills and night sweats of 1-week duration. The thoracic scan reveals lymph node enlargement in the left axilla and pleural effusion and the histological study revealed a necrotising granulomatous lymphadenitis. It was decided to initiate antituberculous drugs with a good response. EPTB is a difficult diagnostic because lymph nodes contain few tubercle bacilli, leading to a low sensitivity of smear microscopy detection. The introduction of antituberculous agents is the cornerstone of management of such infections and, occasionally, it is the only way to make a diagnosis.
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http://dx.doi.org/10.1136/bcr.11.2010.3548DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3062882PMC
February 2011