Publications by authors named "Amina Rhouati"

11 Publications

  • Page 1 of 1

Analysis of Recent Bio-/Nanotechnologies for Coronavirus Diagnosis and Therapy.

Sensors (Basel) 2021 Feb 20;21(4). Epub 2021 Feb 20.

Laboratoire BAE, Université de Perpignan Via domitia, 66860 Perpignan, France.

Despite barrier measures and physical distancing tailored by the populations worldwide, coronavirus continues to spread causing severe health and social-economic problems. Therefore, researchers are focusing on developing efficient detection and therapeutic platforms for SARS-CoV2. In this context, various biotechnologies, based on novel molecules targeting the virus with high specificity and affinity, have been described. In parallel, new approaches exploring nanotechnology have been proposed for enhancing treatments and diagnosis. We discuss in the first part of this review paper, the different biosensing and rapid tests based on antibodies, nucleic acids and peptide probes described since the beginning of the pandemic. Furthermore, given their numerous advantages, the contribution of nanotechnologies is also highlighted.
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http://dx.doi.org/10.3390/s21041485DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7924586PMC
February 2021

Development of a label-free electrochemical aptasensor based on diazonium electrodeposition: Application to cadmium detection in water.

Anal Biochem 2021 01 17;612:113956. Epub 2020 Sep 17.

Laboratory of Biosensors, Analysis and Environment (BAE), University of Perpignan Via Domitia, Perpignan, France; Higher National School of Biotechnology, Constantine, Algeria. Electronic address:

In this study we have developed a new aptasensor for cadmium (Cd) detection in water. Gold electrode surface has been chemically modified by electrochemical reduction of diazonium salt (CMA) with carboxylic acid outward from the surface. This was used for amino-modified cadmium aptamer immobilization through carbodiimide reaction. Chemical surface modification was characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). This latter was also used for Cd detection. The aptasensor has exhibited a good linear relationship between the logarithm of the Cd concentration and the impedance changes in the range from 10 to 10 M with a correlation R of 0.9954. A high sensitivity was obtained with a low limit of detection (LOD) of 2.75*10 M. Moreover, the developed aptasensor showed a high selectivity towards Cd when compared to other interferences such as Hg, Pb and Zn. The developed aptasensor presents a simple and sensitive approach for Cddetection in aqueous solutions with application for trace Cd detection in spring water samples.
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http://dx.doi.org/10.1016/j.ab.2020.113956DOI Listing
January 2021

MIP-Based Impedimetric Sensor for Detecting Dengue Fever Biomarker.

Appl Biochem Biotechnol 2020 Aug 26;191(4):1384-1394. Epub 2020 Feb 26.

Interdisciplinary Research Centre in Biomedical Materials (IRCBM), COMSATS University Islamabad, Lahore Campus, Lahore, 54600, Pakistan.

In this study, molecular imprinted polymer (MIP)-based impedimetric sensor has been developed to detect dengue infection at an early stage. Screen-printed carbon electrode (SPCE) was modified with electrospun nanofibers of polysulfone (PS) and then, coated with dopamine while using NS1 (non-structural protein 1-a specific and sensitive biomarker for dengue virus infection) as template during polymerization. The self-polymerization of dopamine at room temperature helps to retain exact structure of template (NS1) which results in generating geometrically fit imprinted sites for specific detection of target analyte. The electrochemical properties of MIP-modified SPCEs were studied by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) at every step of modification. Under optimal conditions, impedimetric measurements showed linear response in the range from 1 to 200 ng/mL. The developed sensor can selectively detect NS1 concentrations as low as 0.3 ng/mL. Moreover, impedimetric sensor system was also employed for NS1 determination in real human serum samples and satisfying recoveries varying from 95 to 97.14% were obtained with standard deviations of less than 5%.
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http://dx.doi.org/10.1007/s12010-020-03285-yDOI Listing
August 2020

A Review of the Construction of Nano-Hybrids for Electrochemical Biosensing of Glucose.

Biosensors (Basel) 2019 Mar 25;9(1). Epub 2019 Mar 25.

BAE: Biocapteurs-Analyses-Environnement, Universite de Perpignan ViaDomitia, 52 Avenue Paul Alduy, 66860 Perpignan CEDEX, France.

Continuous progress in the domain of nano and material science has led to modulation of the properties of nanomaterials in a controlled and desired fashion. In this sense, nanomaterials, including carbon-based materials, metals and metal oxides, and composite/hybrid materials have attracted extensive interest with regard to the construction of electrochemical biosensors. The modification of a working electrode with a combination of two or three nanomaterials in the form of nano-composite/nano-hybrids has revealed good results with very good reproducibility, stability, and improved sensitivity. This review paper is focused on discussing the possible constructs of nano-hybrids and their subsequent use in the construction of electrochemical glucose biosensors.
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http://dx.doi.org/10.3390/bios9010046DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468850PMC
March 2019

Photoinduced discharge of electrons stored in a TiO2-MWCNT composite to an analyte: application to the fluorometric determination of hydrogen peroxide, glucose and aflatoxin B1.

Mikrochim Acta 2017 12 6;185(1):26. Epub 2017 Dec 6.

Interdisciplinary Research Centre in Biomedical Materials (IRCBM), COMSATS Institute of Information Technology (CIIT), Lahore, 54000, Pakistan.

The authors describe an analytical detection scheme based on the use of multiwalled carbon nanotubes (MWCNTs) that accept and store electrons upon contact with photo-irradiated TiO nanoparticles (TiO-NPs). The Fermi level equilibration with photo-irradiated TiO-NPs has a storage value of 0.35 mM of electrons per 120 mg·L of MWCNTs. The stored electrons can be discharged on demand upon addition of electron acceptors to the TiO-NP/MWCNT composite. These findings are applied to detect the quencher hydrogen peroxide. HO also is produced on enzymatic action of glucose oxidase on glucose, and this enables glucose also to be quantified by using the TiO-NP/MWCNT fluorescent nanoprobe. The wide scope of the method also is demonstrated by an assay for aflatoxin B1 that is making use of an FAM-labeled aptamer where the FAM fluorophore on the aptamer quenches the emission of the nanoprobe. The following analytical linear ranges and limits of detection are found: HO: 0.1-100 μM and 15 nM; glucose: 5-200 μM and 0.5 μM; aflatoxin: 0.1-40 ng·mL and 0.02 ng·mL. The method was applied to the determination of glucose in human serum. Graphical abstract The assays demonstrated in (b) and (c) are based on the fluorescence quenching ability of MWCNTs-TiO. In the presence of the target (analyte), the fluorescence is restored and the target concentration is determined from the percentage of fluorescence recovery.
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http://dx.doi.org/10.1007/s00604-017-2583-7DOI Listing
December 2017

Nano-Aptasensing in Mycotoxin Analysis: Recent Updates and Progress.

Toxins (Basel) 2017 10 28;9(11). Epub 2017 Oct 28.

BAE: Biocapteurs-Analyses-Environnement, Universite de Perpignan Via Domitia, 52 Avenue Paul Alduy, 66860 Perpignan CEDEX, France.

Recent years have witnessed an overwhelming integration of nanomaterials in the fabrication of biosensors. Nanomaterials have been incorporated with the objective to achieve better analytical figures of merit in terms of limit of detection, linear range, assays stability, low production cost, etc. Nanomaterials can act as immobilization support, signal amplifier, mediator and artificial enzyme label in the construction of aptasensors. We aim in this work to review the recent progress in mycotoxin analysis. This review emphasizes on the function of the different nanomaterials in aptasensors architecture. We subsequently relate their features to the analytical performance of the given aptasensor towards mycotoxins monitoring. In the same context, a critically analysis and level of success for each nano-aptasensing design will be discussed. Finally, current challenges in nano-aptasensing design for mycotoxin analysis will be highlighted.
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http://dx.doi.org/10.3390/toxins9110349DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5705964PMC
October 2017

Label-Free Aptasensors for the Detection of Mycotoxins.

Sensors (Basel) 2016 Dec 18;16(12). Epub 2016 Dec 18.

BAE Laboratory, Université de Perpignan Via Domitia, 52 Avenue Paul Alduy, Perpignan 66860, France.

Various methodologies have been reported in the literature for the qualitative and quantitative monitoring of mycotoxins in food and feed samples. Based on their enhanced specificity, selectivity and versatility, bio-affinity assays have inspired many researchers to develop sensors by exploring bio-recognition phenomena. However, a significant problem in the fabrication of these devices is that most of the biomolecules do not generate an easily measurable signal upon binding to the target analytes, and signal-generating labels are required to perform the measurements. In this context, aptamers have been emerged as a potential and attractive bio-recognition element to design label-free aptasensors for various target analytes. Contrary to other bioreceptor-based approaches, the aptamer-based assays rely on antigen binding-induced conformational changes or oligomerization states rather than binding-assisted changes in adsorbed mass or charge. This review will focus on current designs in label-free conformational switchable design strategies, with a particular focus on applications in the detection of mycotoxins.
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http://dx.doi.org/10.3390/s16122178DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5191157PMC
December 2016

Ligand Assisted Stabilization of Fluorescence Nanoparticles; an Insight on the Fluorescence Characteristics, Dispersion Stability and DNA Loading Efficiency of Nanoparticles.

J Fluoresc 2016 Jul 21;26(4):1407-14. Epub 2016 May 21.

BAE: Biocapteurs-Analyses-Environnement, Universite de Perpignan Via Domitia, 52 Avenue Paul Alduy, 66860, Perpignan, Cedex, France.

This work reports on the ligand assisted stabilization of Fluospheres® carboxylate modified nanoparticles (FCMNPs), and subsequently investigation on the DNA loading capacity and fluorescence response of the modified particles. The designed fluorescence bioconjugate was characterized with enhanced fluorescence characteristics, good stability and large surface area with high DNA loading efficiency. For comparison purpose, bovine serum albumin (BSA) and polyethylene glycol (PEG) with three different length strands were used as cross linkers to modify the particles, and their DNA loading capacity and fluorescence characteristics were investigated. By comparing the performance of the particles, we found that the most improved fluorescence characteristics, enhanced DNA loading and high dispersion stability were obtained, when employing PEG of long spacer arm length. The designed fluorescence bioconjugate was observed to maintain all its characteristics under varying pH over an extended period of time. These types of bioconjugates are in great demand for fluorescence imaging and in vivo fluorescence biomedical application, especially when most of the as synthesized fluorescence particles cannot withstand to varying in vivo physiological conditions with decreases in fluorescence response and DNA loading efficiency.
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http://dx.doi.org/10.1007/s10895-016-1832-1DOI Listing
July 2016

Recent advances and achievements in nanomaterial-based, and structure switchable aptasensing platforms for ochratoxin A detection.

Sensors (Basel) 2013 Nov 6;13(11):15187-208. Epub 2013 Nov 6.

BIOMEM, Université de Perpignan, 52 Avenue Paul Alduy, Perpignan Cedex 66860, France.

Aptamer-based bioreceptors that can easily adopt their surroundings have captured the attention of scientists from a wide spectrum of domains in designing highly sensitive, selective and structure switchable sensing assays. Through elaborate design and chemical functionalization, numerous aptamer-based assays have been developed that can switch their conformation upon incubation with target analyte, resulting in an enhanced output signal. To further lower the detection limits to picomolar levels, nanomaterials have attracted great interest in the design of aptamer-based sensing platforms. Associated to their unique properties, nanomaterials offer great promise for numerous aptasensing applications. This review will discuss current research activities in the aptasensing with typical example of detection of ochratoxin A (OTA). OTA, a secondary fungal metabolite, contaminates a variety of food commodities, and has several toxicological effects such as nephrotoxic, hepatotoxic, neurotoxic, teratogenic and immunotoxic activities. The review will introduce advances made in the methods of integrating nanomaterials in aptasensing, and will discuss current conformational switchable design strategies in aptasensor fabrication methodologies.
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http://dx.doi.org/10.3390/s131115187DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3871093PMC
November 2013

Aptamers: a promosing tool for ochratoxin A detection in food analysis.

Toxins (Basel) 2013 Nov 5;5(11):1988-2008. Epub 2013 Nov 5.

IMAGES, Université de Perpignan, 52 Avenue Paul Alduy, Perpignan Cedex 66860, France.

The contamination of food and feed by mycotoxins has become an increasingly serious problem. Mycotoxins represent a major risk to human and animal health, as well as economics. Herein, we focus on Ochratoxin A (OTA), which is one of the most common mycotoxins contaminating feed and foodstuffs. OTA is a secondary metabolite produced by various Aspergillus and Penicillium strains. Upon ingestion, OTA has a number of acute and chronic toxic effects. It is nephrotoxic, teratogenic, immunosuppressive, and carcinogenic (group 2B). As a consequence, some regulatory limits have been introduced on the levels of OTA in several commodities. The toxic nature of OTA demands highly sensitive and selective monitoring techniques to protect human and animal health. As alternative to traditional analytical techniques, biochemical methods for OTA analysis have attained great interest in the last few decades. They are mainly based on the integration of antibodies or aptamers as biorecognition elements in sensing platforms. However, aptamers have gained more attention in affinity-based assays because of their high affinity, specificity, stability, and their easy chemical synthesis. In this brief review, we present an overview of aptamer-based assays and their applications in OTA purification and detection, appeared in the literature in the last five years.
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http://dx.doi.org/10.3390/toxins5111988DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3847711PMC
November 2013

Immobilization of enzymes on ethynyl-modified electrodes via click chemistry.

Methods Mol Biol 2013 ;1051:209-16

IMAGES EA 4218, University of Perpignan, Perpignan Cedex, France.

This paper describes a novel, simple, and versatile protocol for covalent immobilization of enzyme on electrode. The immobilization method is based on the combination of diazonium salt electrografting and click chemistry. The ethynyl-terminated monolayers are obtained by diazonium salt electrografting, then, in the presence of copper (I) catalyst, the ethynyl modified surfaces reacts efficiently and rapidly with enzyme bearing an azide function (azido-enzyme), thus forming a covalent 1,2,3-triazole linkage by means of click chemistry. The ethynyl-terminated film preserves the activity of the immobilized enzyme. The click chemistry along with binary film of diazonium salts offers a variety of good characteristics including high sensitivity, good repeatability and reusability, rapid response and long term stability of the system. Thus, because of the chemoselective reactivity and quantitative yield of the click reaction, an ethynyl-terminated monolayer can be treated as a general platform for obtaining reliable coverage of a wide range of azido-terminated species of interest for various sensing applications.
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http://dx.doi.org/10.1007/978-1-62703-550-7_13DOI Listing
February 2014