Publications by authors named "Alok Dubey"

18 Publications

  • Page 1 of 1

Malondialdehyde, an Oxidative Stress Marker in Oral Squamous Cell Carcinoma-A Systematic Review and Meta-Analysis.

Curr Issues Mol Biol 2021 Aug 28;43(2):1019-1035. Epub 2021 Aug 28.

Division of Oral Pathology, Department of Maxillofacial Surgery and Diagnostic Sciences, College of Dentistry, Jazan University, Jazan 45142, Saudi Arabia.

Objective: To qualitative and quantitatively review published literature assessing the oxidative stress marker malondialdehyde (MDA) in oral squamous cell carcinoma (OSCC).

Methodology: Pubmed (MeSH), Science Direct, Scopus, Web of Science, Willey Online Library, Cochrane, and Cross Reference were searched for studies assessing MDA levels in OSCC samples.

Results: From the 1008 articles identified, 849 were excluded based on title and abstract screening due to duplication and irrelevance to the topic of interest. Full-text assessment of the remaining 159 articles led to the inclusion of only 46 articles that satisfied the selection criteria. Of these, only 26 studies had data compatible for quantitative analysis. The MDA levels in OSCC groups are significantly increased ( < 0.00001) in plasma, serum, and saliva samples in the majority of the studies evaluated. In contrast, MDA levels in OSCC tissue samples are significantly attenuated ( < 0.00001) compared to healthy controls, supported by fewer studies.

Conclusions: The augmented MDA levels in plasma, serum, and saliva samples of the OSCC reflect the heightened oxidative stress level accurately. Further studies are required to understand the attenuated MDA levels in the tissue samples of OSCC. Correlation analysis between MDA levels with established clinicopathological prognostic markers could aid in formulating oxidative stress-based prognostication and treatment planning.
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http://dx.doi.org/10.3390/cimb43020072DOI Listing
August 2021

Plasmodium's journey through the Anopheles mosquito: A comprehensive review.

Biochimie 2021 Feb 17;181:176-190. Epub 2020 Dec 17.

Department of Zoology, School of Basic Sciences, Central University of Punjab, Ghudda, 151401, India. Electronic address:

The malaria parasite has an extraordinary ability to evade the immune system due to which the development of a malaria vaccine is a challenging task. Extensive research on malarial infection in the human host particularly during the liver stage has resulted in the discovery of potential candidate vaccines including RTS,S/AS01 and R21. However, complete elimination of malaria would require a holistic multi-component approach. In line with this, under the World Health Organization's PATH Malaria Vaccine Initiative (MVI), the research focus has shifted towards the sexual stages of malaria in the mosquito host. Last two decades of scientific research obtained seminal information regarding the sexual/mosquito stages of the malaria. This updated and comprehensive review would provide the basis for consolidated understanding of cellular, biochemical, molecular and immunological aspects of parasite transmission right from the sexual stage commitment in the human host to the sporozoite delivery back into subsequent vertebrate host by the female Anopheles mosquito.
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http://dx.doi.org/10.1016/j.biochi.2020.12.009DOI Listing
February 2021

Propolis: A Smart Supplement for an Intracanal Medicament.

Int J Clin Pediatr Dent 2017 Oct-Dec;10(4):324-329. Epub 2017 Feb 27.

Postdradugate Student, Department of Pedodontics and Preventive Dentistry Rungta College of Dental Sciences & Research, Bhilai Chhattisgarh, India.

Introduction: One of the most important factors for successful endodontic therapy is root canal cleaning. The difficulty involved in eliminating microorganisms, as well as their residual presence, warrants the use of root canal dressings after bio-mechanical preparation.

Aim: The aim of the study was to compare the diffusion ability between nonalcoholic calcium hydroxide-propolis paste, calcium hydroxide-saline paste, and calcium hydroxide-propylene glycol paste.

Materials And Methods: For this proposed study, single-rooted extracted permanent teeth were randomly divided into three groups to fill the canals: group I: Calcium hydroxide-propylene glycol paste, group II: Calcium hydroxide-saline paste, and group III: Calcium hydroxide-propolis paste. After complete filing of the root canal, the pH values of the solutions in the flasks are measured at an interval of 3, 24, 72, 168 hours.

Results: After 168-hour interval, it was noticed that the mean pH obtained by calcium hydroxide-propolis paste was 10.54 (± 0.38), which was greater than calcium hydroxide-propylene glycol paste 9.70 (± 0.45) and calcium hydroxide-saline paste 9.16 (± 0.30) consecutively.

Conclusion: The nonalcoholic calcium hydroxide-propolis paste used during the study was able to diffuse through the dentinal tubules. Thus, it can be used as a vehicle for calcium hydroxide. Baranwal R, Duggi V, Avinash A, Dubey A, Pagaria S, Munot H. Propolis: A Smart Supplement for an Intracanal Medicament. Int J Clin Pediatr Dent 2017; 10(4):324-329.
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http://dx.doi.org/10.5005/jp-journals-10005-1459DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5789133PMC
February 2017

EGFR family and Src family kinase interactions: mechanics matters?

Curr Opin Cell Biol 2018 04 28;51:97-102. Epub 2017 Dec 28.

Mechanobiology Institute, National University of Singapore, 5A Engineering Drive 1, Singapore 117411; Department of Biological Sciences, Columbia University, 1212 Amsterdam Avenue, New York, NY 10027, USA. Electronic address:

Receptor tyrosine kinases (RTKs), such as the EGF receptor family, and adhesion molecules, such as integrins, have historically been viewed to have distinctly separable roles in the cell. In this classical view, integrins mediate mechanical interactions between the cell and its surrounding extracellular matrix while RTKs handle signaling to modulate cellular behavior. Although crosstalk between these receptor pathways has been known to exist for a long time, this has generally been attributed to effects significantly downstream from the receptors themselves. In recent years, however, EGFR family RTKs have been found to directly participate in integrin-mediated force sensing, revealing a more complex interplay among these cellular components than originally appreciated. Here we briefly review the classical understanding of EGFR family RTK signaling and then provide a broadened perspective based on recent results.
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http://dx.doi.org/10.1016/j.ceb.2017.12.003DOI Listing
April 2018

Restoring the voids of voices by signs and gestures, in dentistry: A cross-sectional study.

J Indian Soc Pedod Prev Dent 2017 Apr-Jun;35(2):115-122

Department of Pedodontics and Preventive Dentistry, Rungta College of Dental Sciences, Bhilai, Chhattisgarh, India.

Aims: To help dentists to communicate with the hearing impaired patients, reach an accurate diagnosis and explain the treatment plan by learning some signs and gestures used in the nonverbal communication (NVC) and by devising some new signs and gestures related to dentistry which shall be easy to learn and understand both by the hearing impaired patients and the dentists.

Settings And Design: The study was carried out on 100 hearing impaired students in the age group of 10-14 years in two special schools for hearing impaired children located in two different states of India, where different spoken languages and different sign languages are used. One dentist (expert dentist) was trained in the NVC and the other dentist (non expert dentist) had no knowledge of this type of communication, communicated the same sets of statements related to dentistry, to the hearing impaired children. One1 translator was assigned to judge their interactions. Students were asked to tell the interpreter at the end of each signed interaction what they understood from the statement conveyed to them by both the dentists.

Statistical Analysis Used: All data collected were subjected to statistical analysis using Chi-square test and odds ratio test.

Results: In the special school of 1st state, the nonexpert dentist conveyed only 36.3% of the information correctly to the students, whereas the expert dentist conveyed 83% of the information correctly. In the special school of 2nd state, the nonexpert dentist conveyed only 37.5% of the information correctly to the students, whereas the expert dentist conveyed 80.3% of the information correctly.

Conclusions: Dentists should be made aware of the NVC and signs and gestures related to dentistry should be taught to the hearing impaired students as well as the dental students.
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http://dx.doi.org/10.4103/JISPPD.JISPPD_88_16DOI Listing
March 2018

Dynein Clusters into Lipid Microdomains on Phagosomes to Drive Rapid Transport toward Lysosomes.

Cell 2016 Feb 4;164(4):722-34. Epub 2016 Feb 4.

Department of Biological Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai 400005, India. Electronic address:

Diverse cellular processes are driven by motor proteins that are recruited to and generate force on lipid membranes. Surprisingly little is known about how membranes control the force from motors and how this may impact specific cellular functions. Here, we show that dynein motors physically cluster into microdomains on the membrane of a phagosome as it matures inside cells. Such geometrical reorganization allows many dyneins within a cluster to generate cooperative force on a single microtubule. This results in rapid directed transport of the phagosome toward microtubule minus ends, likely promoting phagolysosome fusion and pathogen degradation. We show that lipophosphoglycan, the major molecule implicated in immune evasion of Leishmania donovani, inhibits phagosome motion by disrupting the clustering and therefore the cooperative force generation of dynein. These findings appear relevant to several pathogens that prevent phagosome-lysosome fusion by targeting lipid microdomains on phagosomes.
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http://dx.doi.org/10.1016/j.cell.2015.12.054DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4752818PMC
February 2016

Assessment of the Risk Factors for Oro-Dental Injuries to Occur during General Anesthesia and Measures taken by Anesthesiologist to Prevent them.

J Int Oral Health 2015 Jul;7(7):77-9

Private practioner, Department of Oral Medicine and Radiology.

Background And Aim: Anesthesiologist gain access to the airway passage orally with the help of laryngoscope. Dental trauma can occur during different steps in anesthesia. The aim of the study is to evaluate the risk factor for dental trauma perioperatively and to look for the preventive measures mostly employed by the anesthesiologist to prevent dental insult.

Materials And Methods: The present study involved 40 anesthetists working in private hospitals using simple random sampling. They were asked to answer a questionnaire designed to look for the risk factors that makes tooth most prone for injury and find out the measures that anesthesiologists follow to prevent such injuries to occur. Codes were placed for the obtained data and Medcalc statistical package was used for analysis.

Results: Injuries mostly occur during intubation with a laryngoscope in patients where there is limited visibility to the hypopharynx. Maxillary left central incisor was mostly affected. Some anesthetists used plastic blades or curved blades to prevent such injury.

Conclusion: Patient with poor dentition and history of difficult intubation should be sent for pre-operative dental evaluation to prevent dental injuries.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4513781PMC
July 2015

Nurses' experience and role in infants with natal teeth.

Iran J Nurs Midwifery Res 2015 May-Jun;20(3):409

Department of Rasa Shastra, Shri Dhanwantri Ayurvedic Medical College and Research Centre, Samari, Mathura, Uttra Pradesh, India.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4462069PMC
June 2015

Clinical Consequences of Untreated Dental Caries Evaluated with the Pulpal Involvement-Roots-Sepsis Index in the Primary Dentition of School Children from the Raipur and Durg Districts, Chhattisgarh State, India.

Med Princ Pract 2014 Nov 26. Epub 2014 Nov 26.

Department of Pedodontics and Preventive Dentistry, Rungta College of Dental Sciences and Research, Bhilai, India.

Objective: To evaluate the prevalence of the clinical consequences of untreated dental caries in primary dentition among 6- to 8-year-old children using the prs [pulpal involvement (p), roots (r) and sepsis (s)] index and record the distribution of prs among boys and girls. Subjects and Methods: The study included a sample of 371 children aged 6-8 years with at least one carious primary molar tooth, who had been randomly selected from schools in the districts of Raipur and Durg, Chhattisgarh State, India. We recorded the presence of decayed, extracted and filled (i.e. def) teeth. The prevalence of the consequences of untreated dental caries was evaluated using the prs index. The data were statistically analyzed to express the prevalence of prs among boys and girls and the distribution of individual prs codes in the sample population. Results: The prevalence of carious lesions was very high (87%) and teeth with clinical consequences of untreated dental caries were found in 49.3% of the children. The prs distribution in children who were 8 years old (40.9%) was higher than in the 6-year-old children (25.6%). In the group of 8-year-olds, the occurrence of prs was greater among the boys whereas in the 6- and 7-year-old group, it was greater among the girls. Conclusion: The prs index is a valuable tool for measuring the clinical consequences of untreated dental caries. It could be useful for epidemiological studies and provide relevant information regarding treatment needs. © 2014 S. Karger AG, Basel.
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http://dx.doi.org/10.1159/000369067DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5588195PMC
November 2014

Surgical removal of coronal fragment of tooth embedded in lower lip and esthetic management of fractured crown segment.

Int J Clin Pediatr Dent 2014 Jan 26;7(1):65-8. Epub 2014 Apr 26.

Postgraduate Student, Department of Oral Medicine, Rungta College of Dental Sciences and Research, Bhilai, Chhattisgarh, India.

Dental fractures of the permanent maxillary anterior teeth are relatively frequent accidents during childhood. The Efficient diagnosis and treatment of dental injury are important elements in clinical dentistry. This article describes a case of trauma in permanent right central maxillary incisors with tooth fragments embedded in the lower lip. Thorough clinical examination followed by soft tissue radiographs confirmed the presence of a fractured incisal fragment, which was surgically retrieved under local anesthesia. Direct composite restoration was placed. After finishing and polishing, an esthetic and natural-looking restoration was achieved; this completely satisfied the functional and esthetic expectation of the patient and dental team. How to cite this article: Avinash A, Dubey A, Singh RK, Prasad S. Surgical Removal of Coronal Fragment of Tooth Embedded in Lower Lip and Esthetic Management of Fractured Crown Segment. Int J Clin Pediatr Dent 2014;7(1):65-68.
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http://dx.doi.org/10.5005/jp-journals-10005-1238DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4144052PMC
January 2014

Assessment of oral side effects of Antiepileptic drugs and traumatic oro-facial injuries encountered in Epileptic children.

J Int Oral Health 2014 Apr 26;6(2):126-8. Epub 2014 Apr 26.

Department of Pedodontics & Preventive Dentistry, Rungta College of Dental Sciences and Research, Bhilai, Chhattisgarh, lndia.

Background: Epilepsy is a chronic disorder with unpredictably recurring seizure. Uncontrolled attacks can put patients at risk of suffering oro-facial trauma. Antiepileptic drugs (AED) provide satisfactory control of seizures in most of the patients with epilepsy. However use of AED has been found to cause many side effects inclusive of side effects in the oral cavity also.

Materials & Methods: This study was conducted on 150 epileptic children, who were on anti epileptic medication for one year.

Results: Gingival over growth was seen as common side effect of the AED drugs. Lip and cheek biting were the most common soft tissue injury, while tooth fracture was the most common hard tissue dental injury.

Conclusion: General physicians, physicians & dentists should be well aware of the potential side effects of AED. A Dentist should be well versed and trained to manage oro-facial injuries in the emergency department. How to cite the article: Ghafoor PA, Rafeeq M, Dubey A. Assessment of oral side effects of Antiepileptic drugs and traumaticoro-facial injuries encountered in Epileptic children. J Int Oral Health 2014;6(2):126-8.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4037794PMC
April 2014

Reconstitution of microtubule-dependent organelle transport.

Methods Enzymol 2014 ;540:231-48

Department of Biological Sciences, Tata Institute of Fundamental Research, Colaba, Mumbai, Maharashtra, India. Electronic address:

Microtubule (MT)-based motor proteins transport many cellular factors to their functionally relevant locations within cells, and defects in transport are linked to human disease. Understanding the mechanism and regulation of this transport process in living cells is difficult because of the complex in vivo environment and limited means to manipulate the system. On the other hand, in vitro motility assays using purified motors attached to beads does not recapitulate the full complexity of cargo transport in vivo. Assaying motility of organelles in cell extracts is therefore attractive, as natural cargoes are being examined, but in an environment that is more amenable to manipulation. Here, we describe the purification and in vitro MT-based motility of phagosomes from Dictyostelium and lipid droplets from rat liver. These assays have the potential to address diverse questions related to endosome/phagosome maturation, fatty acid regulation, and could also serve as a starting point for reconstituting the motility of other types of organelles.
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http://dx.doi.org/10.1016/B978-0-12-397924-7.00013-3DOI Listing
November 2014

'Mend the bend': a report of two cases of labial dilacerated and palatal dilacerated crown.

BMJ Case Rep 2013 Jul 31;2013. Epub 2013 Jul 31.

Department of Pedodontics, Rungta College of Dental Sciences, Bhilai, Chhattisgarh, India.

Dilaceration is the result of a developmental anomaly in which there has been an abrupt change in the axial inclination between the crown and the root of a tooth. Crown dilaceration of permanent tooth occurs due to the non-axial displacement of the already formed hard tissue portion of the developing crown at an angle to their longitudinal axis due to trauma to the primary predecessors. Thus endo-aesthetic management of such teeth helps in maintaining morphology as well as aesthetics in a growing child. This article depicts two cases of severely dilacerated crown.
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http://dx.doi.org/10.1136/bcr-2013-200291DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3736203PMC
July 2013

Simplified design and precautionary measures in fabrication of a feeding obturator for a newborn with cleft lip and palate.

BMJ Case Rep 2013 Jun 16;2013. Epub 2013 Jun 16.

Department of Pedodontics, Rungta College of Dental Sciences, Bhilai, Chhattisgarh, India.

The immediate problem to be addressed in a new born with cleft lip and palate is the interference of feeding. Here we present a case of cleft lip and palate for which a feeding obturator was made to facilitate feeding till the defect could be surgically repaired. The design was modified by placing orthodontic wire on the acrylic plate for attaching the ties of mouth mask. Different precautionary measures undertaken while making impression for infant have also been described. Feeding appliance improved the sucking ability of the infant. The attachment of mouth-mask ties help in easy retrieval of the obturator intraorally in case of airway obstruction.
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http://dx.doi.org/10.1136/bcr-2013-010465DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3703091PMC
June 2013

Missed diagnosis: blunt abdominal injury-case report.

Pac Health Dialog 2010 Sep;16(2):70-4

Colonial War Memorial Hospital, Suva.

Acute pancreatitis is an uncommon finding in children and therefore one must have a high index of suspicion when evaluating children with blunt abdominal trauma. It can cause severe morbidity and mortality and requires one to be vigilant to make the diagnosis and to ensure appropriate management. We report on a case of acute pancreatitis that had diagnostic and management dilemmas at Colonial War Memorial Hospital (CWMH) in Fiji.
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September 2010

HindIII-based restriction fragment length polymorphism in hemophilic and nonhemophilic patients.

J Nat Sci Biol Med 2010 Jul;1(1):25-8

Division of Cellulose and Paper, Forest Research Institute, P.O., IPE, Kaulagarh Road, Dehradun, Uttarakhand, India.

Hemophilia A is most common recessively inherited bleeding disorder, which affect one in five thousand male births throughout the world. In most of the hemophilic A patients, no common mutation is easily identifiable. This limitation has been overcome by the use of polymorphic DNA marker, i.e., restriction fragment length polymorphism (RFLP). This marker of polymorphism could only be detected by amplifying the polymorphic region and digestion the polymerase chain reaction (PCR) product with the restriction enzyme (PCR-RFLP), i.e., HindIII. The polymorphic region of HindIII is 608 bp in length and after the restriction digestion, different sizes of fragments, i.e., 427 and 181 bp were, respectively, obtained. However, in homozygous (+/+) condition three bands of 427, 100, and 81 bp were obtained and in the other negative allelic homozygous condition (-/-) two bands of 427 and 181 bp were obtained. Similarly fragments of different sizes, i.e., 427, 181, 100, and 81 bp were obtained in heterozygous conditions. Therefore, in this study, we have analyzed the factor VIII gene in the 17 different families using restriction enzyme HindIII-based RFLP molecular marker technique. Out of these, the observed heterozygosity for HindIII was found 47.5%, whereas, for positive allele it was 26%, and for negative allele the frequency was 74%.
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http://dx.doi.org/10.4103/0976-9668.71669DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3217284PMC
July 2010

Epidural hematoma complicating high thoracic epidural catheter placement intended for cardiac surgery.

J Cardiothorac Vasc Anesth 2004 Dec;18(6):759-62

Department of Cardiothoracic Anaesthesiology, Military Hospital (CTC), Pune, India.

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http://dx.doi.org/10.1053/j.jvca.2004.08.015DOI Listing
December 2004

Nasotracheal intubation: an unusual cause of palatal perforation in an insulin dependent diabetes mellitus patient.

J Clin Pediatr Dent 2004 ;29(1):79-81

Department of Oral Medicine and Radiology, VYWS, Dental College and Hospital, Amravati, India.

A case of palatal perforation occurring in 7-year-old girl with IDDM due to nasotracheal intubation is reported. The child, who was not previously diagnosed of IDDM, was brought to hospital in comatose stage and was put on nasotracheal tube for maintaining respiration. This paper highlights the link between IDDM and palatal perforation communicating the nasal cavity due to naso-tracheal intubation.
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http://dx.doi.org/10.17796/jcpd.29.1.g31404575941287lDOI Listing
December 2004
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