Alice Ting

Alice Ting

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Alice Ting

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Directed evolution improves the catalytic efficiency of TEV protease.

Nat Methods 2020 Feb 9;17(2):167-174. Epub 2019 Dec 9.

Departments of Genetics, Biology and Chemistry, Stanford University, Stanford, CA, USA.

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http://dx.doi.org/10.1038/s41592-019-0665-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7004888PMC
February 2020

Publisher Correction: Directed evolution improves the catalytic efficiency of TEV protease.

Nat Methods 2020 Feb;17(2):242

Departments of Genetics, Biology and Chemistry, Stanford University, Stanford, CA, USA.

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http://dx.doi.org/10.1038/s41592-019-0729-8DOI Listing
February 2020

Author Correction: Efficient proximity labeling in living cells and organisms with TurboID.

Nat Biotechnol 2020 Jan;38(1):108

Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

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http://dx.doi.org/10.1038/s41587-019-0355-0DOI Listing
January 2020

Cell-Surface Proteomic Profiling in the Fly Brain Uncovers Wiring Regulators.

Cell 2020 Jan 16;180(2):373-386.e15. Epub 2020 Jan 16.

Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA. Electronic address:

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http://dx.doi.org/10.1016/j.cell.2019.12.029DOI Listing
January 2020

Atlas of Subcellular RNA Localization Revealed by APEX-Seq.

Cell 2019 Jul 20;178(2):473-490.e26. Epub 2019 Jun 20.

Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA; Department of Chemistry, Stanford University, Stanford, CA 94305, USA; Department of Biology, Stanford University, Stanford, CA 94305, USA; Chan Zuckerberg Biohub, San Francisco, CA 94158, USA. Electronic address:

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http://dx.doi.org/10.1016/j.cell.2019.05.027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6786773PMC
July 2019

TurboID-based proximity labeling reveals that UBR7 is a regulator of N NLR immune receptor-mediated immunity.

Nat Commun 2019 07 19;10(1):3252. Epub 2019 Jul 19.

Department of Plant Biology and The Genome Center, College of Biological Sciences, University of California, Davis, Davis, CA, 95616, USA.

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http://dx.doi.org/10.1038/s41467-019-11202-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6642208PMC
July 2019

Directed Evolution of Split APEX2 Peroxidase.

ACS Chem Biol 2019 04 8;14(4):619-635. Epub 2019 Mar 8.

Department of Chemistry , Massachusetts Institute of Technology , Cambridge , Massachusetts 02139 , United States.

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http://pubs.acs.org/doi/10.1021/acschembio.8b00919
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http://dx.doi.org/10.1021/acschembio.8b00919DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6548188PMC
April 2019

Molecular tools for imaging and recording neuronal activity.

Nat Chem Biol 2019 02 18;15(2):101-110. Epub 2019 Jan 18.

Departments of Genetics, Biology, and Chemistry, Stanford University, Stanford, CA, USA.

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http://dx.doi.org/10.1038/s41589-018-0207-0DOI Listing
February 2019

Efficient proximity labeling in living cells and organisms with TurboID.

Nat Biotechnol 2018 10 20;36(9):880-887. Epub 2018 Aug 20.

Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

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http://www.nature.com/doifinder/10.1038/nbt.4201
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http://dx.doi.org/10.1038/nbt.4201DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6126969PMC
October 2018

Proximity labeling: spatially resolved proteomic mapping for neurobiology.

Curr Opin Neurobiol 2018 06 8;50:17-23. Epub 2017 Nov 8.

Department of Chemistry, Stanford University, Stanford, CA 94305, USA; Department of Biology, Stanford University, Stanford, CA 94305, USA; Department of Genetics, Stanford University, Stanford, CA 94305, USA. Electronic address:

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http://dx.doi.org/10.1016/j.conb.2017.10.015DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6726430PMC
June 2018

Antibodies to biotin enable large-scale detection of biotinylation sites on proteins.

Nat Methods 2017 Dec 16;14(12):1167-1170. Epub 2017 Oct 16.

Proteomics, Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.

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http://dx.doi.org/10.1038/nmeth.4465DOI Listing
December 2017

Live-cell mapping of organelle-associated RNAs via proximity biotinylation combined with protein-RNA crosslinking.

Elife 2017 12 14;6. Epub 2017 Dec 14.

Department of Chemistry, Massachusetts Institute of Technology, Cambridge, United States.

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http://dx.doi.org/10.7554/eLife.29224DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5730372PMC
December 2017

Time-gated detection of protein-protein interactions with transcriptional readout.

Elife 2017 11 30;6. Epub 2017 Nov 30.

Department of Genetics, Stanford University, Stanford, United States.

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http://dx.doi.org/10.7554/eLife.30233DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5708895PMC
November 2017

A light- and calcium-gated transcription factor for imaging and manipulating activated neurons.

Nat Biotechnol 2017 Sep 26;35(9):864-871. Epub 2017 Jun 26.

Departments of Genetics, Biology, and Chemistry, Stanford University, Stanford, California, USA.

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http://dx.doi.org/10.1038/nbt.3909DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5595644PMC
September 2017

Electron microscopy using the genetically encoded APEX2 tag in cultured mammalian cells.

Nat Protoc 2017 Sep 10;12(9):1792-1816. Epub 2017 Aug 10.

Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

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http://www.nature.com/doifinder/10.1038/nprot.2017.065
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http://dx.doi.org/10.1038/nprot.2017.065DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5851282PMC
September 2017

The Dopamine Transporter Recycles via a Retromer-Dependent Postendocytic Mechanism: Tracking Studies Using a Novel Fluorophore-Coupling Approach.

J Neurosci 2017 09 28;37(39):9438-9452. Epub 2017 Aug 28.

Brudnick Neuropsychiatric Research Institute, University of Massachusetts Medical School, Worcester, Massachusetts 01604,

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http://dx.doi.org/10.1523/JNEUROSCI.3885-16.2017DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618262PMC
September 2017

Beyond Immunoprecipitation: Exploring New Interaction Spaces with Proximity Biotinylation.

Biochemistry 2017 07 2;56(26):3297-3298. Epub 2017 Jun 2.

Department of Chemistry, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139, United States.

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http://dx.doi.org/10.1021/acs.biochem.7b00466DOI Listing
July 2017

An Approach to Spatiotemporally Resolve Protein Interaction Networks in Living Cells.

Cell 2017 04;169(2):350-360.e12

Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA; Quantitative Biosciences Institute, QBI, University of California, San Francisco, San Francisco, CA 94158, USA; J. David Gladstone Institutes, San Francisco, CA 94158, USA. Electronic address:

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http://dx.doi.org/10.1016/j.cell.2017.03.022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5616215PMC
April 2017

Proximity Biotinylation as a Method for Mapping Proteins Associated with mtDNA in Living Cells.

Cell Chem Biol 2017 Mar 23;24(3):404-414. Epub 2017 Feb 23.

Departments of Genetics, Biology, and Chemistry, Stanford University, Stanford, CA 94305, USA. Electronic address:

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http://dx.doi.org/10.1016/j.chembiol.2017.02.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5886301PMC
March 2017

In Situ Peroxidase Labeling and Mass-Spectrometry Connects Alpha-Synuclein Directly to Endocytic Trafficking and mRNA Metabolism in Neurons.

Cell Syst 2017 02 25;4(2):242-250.e4. Epub 2017 Jan 25.

Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

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http://dx.doi.org/10.1016/j.cels.2017.01.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5578869PMC
February 2017

Proteomic Analysis of Unbounded Cellular Compartments: Synaptic Clefts.

Cell 2016 Aug;166(5):1295-1307.e21

Department of Chemistry, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA. Electronic address:

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http://dx.doi.org/10.1016/j.cell.2016.07.041DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5167540PMC
August 2016

APEX Fingerprinting Reveals the Subcellular Localization of Proteins of Interest.

Cell Rep 2016 05 12;15(8):1837-47. Epub 2016 May 12.

Department of Chemistry, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Korea. Electronic address:

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http://dx.doi.org/10.1016/j.celrep.2016.04.064DOI Listing
May 2016

Spatially resolved proteomic mapping in living cells with the engineered peroxidase APEX2.

Nat Protoc 2016 Mar 11;11(3):456-75. Epub 2016 Feb 11.

Department of Chemistry, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts, USA.

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http://dx.doi.org/10.1038/nprot.2016.018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4863649PMC
March 2016

Proteomic mapping in live Drosophila tissues using an engineered ascorbate peroxidase.

Proc Natl Acad Sci U S A 2015 Sep 11;112(39):12093-8. Epub 2015 Sep 11.

Department of Genetics, Harvard Medical School, Boston, MA 02115; Howard Hughes Medical Institute, Boston, MA 02115

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http://dx.doi.org/10.1073/pnas.1515623112DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4593093PMC
September 2015

A Mechanical Switch Couples T Cell Receptor Triggering to the Cytoplasmic Juxtamembrane Regions of CD3ζζ.

Immunity 2015 Aug 28;43(2):227-39. Epub 2015 Jul 28.

Department of Immunobiology, The University of Arizona College of Medicine, Tucson, AZ 85724, USA; The Arizona Center on Aging, The University of Arizona College of Medicine, Tucson, AZ 85724, USA; The BIO-5 Institute, The University of Arizona College of Medicine, Tucson, AZ 85724, USA. Electronic address:

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http://dx.doi.org/10.1016/j.immuni.2015.06.018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4545397PMC
August 2015

Directed evolution of APEX2 for electron microscopy and proximity labeling.

Nat Methods 2015 Jan 24;12(1):51-4. Epub 2014 Nov 24.

Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

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http://dx.doi.org/10.1038/nmeth.3179DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4296904PMC
January 2015

Proteomic mapping of the human mitochondrial intermembrane space in live cells via ratiometric APEX tagging.

Mol Cell 2014 Jul 4;55(2):332-41. Epub 2014 Jul 4.

Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA. Electronic address:

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http://dx.doi.org/10.1016/j.molcel.2014.06.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4743503PMC
July 2014

Antibiotics induce redox-related physiological alterations as part of their lethality.

Proc Natl Acad Sci U S A 2014 May 6;111(20):E2100-9. Epub 2014 May 6.

Howard Hughes Medical Institute,Departments of Biomedical Engineering andCenter of Synthetic Biology, Boston University, Boston, MA 02215;Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115;Department of Medicine, Boston University School of Medicine, Boston, MA 02118

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http://dx.doi.org/10.1073/pnas.1401876111DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4034191PMC
May 2014

Imaging trans-cellular neurexin-neuroligin interactions by enzymatic probe ligation.

PLoS One 2013 14;8(2):e52823. Epub 2013 Feb 14.

Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America.

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0052823PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3573046PMC
August 2013

Site-specific protein labeling using PRIME and chelation-assisted click chemistry.

Nat Protoc 2013 Aug 25;8(8):1620-34. Epub 2013 Jul 25.

Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

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http://dx.doi.org/10.1038/nprot.2013.096DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4892701PMC
August 2013

Proteomic mapping of mitochondria in living cells via spatially restricted enzymatic tagging.

Science 2013 Mar 31;339(6125):1328-1331. Epub 2013 Jan 31.

Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

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http://dx.doi.org/10.1126/science.1230593DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3916822PMC
March 2013

Quantum dot targeting with lipoic acid ligase and HaloTag for single-molecule imaging on living cells.

ACS Nano 2012 Dec 5;6(12):11080-7. Epub 2012 Dec 5.

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Room 18-496, Cambridge, Massachusetts 02139, USA.

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http://dx.doi.org/10.1021/nn304793zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3528850PMC
December 2012

Engineered ascorbate peroxidase as a genetically encoded reporter for electron microscopy.

Nat Biotechnol 2012 Nov 21;30(11):1143-8. Epub 2012 Oct 21.

Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3699407PMC
http://dx.doi.org/10.1038/nbt.2375DOI Listing
November 2012

Fast, cell-compatible click chemistry with copper-chelating azides for biomolecular labeling.

Angew Chem Int Ed Engl 2012 Jun 3;51(24):5852-6. Epub 2012 May 3.

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Room 18-496, Cambridge, MA 02139, USA.

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http://dx.doi.org/10.1002/anie.201108181DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3517120PMC
June 2012

Site-specific protein modification using lipoic acid ligase and bis-aryl hydrazone formation.

Chembiochem 2012 Apr;13(6):888-94

Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

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http://doi.wiley.com/10.1002/cbic.201100764
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http://dx.doi.org/10.1002/cbic.201100764DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4758125PMC
April 2012

Fluorophore targeting to cellular proteins via enzyme-mediated azide ligation and strain-promoted cycloaddition.

J Am Chem Soc 2012 Feb 14;134(8):3720-8. Epub 2012 Feb 14.

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue. Cambridge, Massachusetts 02139, USA.

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http://dx.doi.org/10.1021/ja208090pDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3306817PMC
February 2012

Diels-Alder cycloaddition for fluorophore targeting to specific proteins inside living cells.

J Am Chem Soc 2012 Jan 5;134(2):792-5. Epub 2012 Jan 5.

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.

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http://dx.doi.org/10.1021/ja209325nDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3381951PMC
January 2012

Imaging protein-protein interactions inside living cells via interaction-dependent fluorophore ligation.

J Am Chem Soc 2011 Dec 18;133(49):19769-76. Epub 2011 Nov 18.

Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

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http://pubs.acs.org/doi/abs/10.1021/ja206435e
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http://dx.doi.org/10.1021/ja206435eDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3547671PMC
December 2011

Structure-guided engineering of a Pacific Blue fluorophore ligase for specific protein imaging in living cells.

Biochemistry 2011 Sep 31;50(38):8221-5. Epub 2011 Aug 31.

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.

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http://pubs.acs.org/doi/abs/10.1021/bi201037r
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http://dx.doi.org/10.1021/bi201037rDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3181222PMC
September 2011

Synthesis of 7-aminocoumarin by Buchwald-Hartwig cross coupling for specific protein labeling in living cells.

Chembiochem 2011 Jan;12(1):65-70

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge MA 02139, USA.

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http://dx.doi.org/10.1002/cbic.201000414DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4857190PMC
January 2011

Imaging activity-dependent regulation of neurexin-neuroligin interactions using trans-synaptic enzymatic biotinylation.

Cell 2010 Oct 7;143(3):456-69. Epub 2010 Oct 7.

Department of Chemistry, Massachusetts Institute of Technology, Cambridge, 02139, USA.

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http://dx.doi.org/10.1016/j.cell.2010.09.025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2966532PMC
October 2010

The heparin-binding domain of HB-EGF mediates localization to sites of cell-cell contact and prevents HB-EGF proteolytic release.

J Cell Sci 2010 Jul 8;123(Pt 13):2308-18. Epub 2010 Jun 8.

Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

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http://dx.doi.org/10.1242/jcs.058321DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2886746PMC
July 2010

A fluorophore ligase for site-specific protein labeling inside living cells.

Proc Natl Acad Sci U S A 2010 Jun 7;107(24):10914-9. Epub 2010 Jun 7.

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.

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http://www.pnas.org/cgi/doi/10.1073/pnas.0914067107
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http://dx.doi.org/10.1073/pnas.0914067107DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2890758PMC
June 2010

InAs(ZnCdS) quantum dots optimized for biological imaging in the near-infrared.

J Am Chem Soc 2010 Jan;132(2):470-1

Massachusetts Institute of Technology, Department of Chemistry, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.

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http://dx.doi.org/10.1021/ja908250rDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2862004PMC
January 2010

Compact biocompatible quantum dots via RAFT-mediated synthesis of imidazole-based random copolymer ligand.

J Am Chem Soc 2010 Jan;132(2):472-83

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge Massachusetts 02139-4307, USA.

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http://dx.doi.org/10.1021/ja908137dDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871316PMC
January 2010

Pyrenebutyrate Leads to Cellular Binding, Not Intracellular Delivery, of Polyarginine-Quantum Dots.

J Phys Chem Lett 2010 ;1:1312-1315

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139; School of Chemistry and Biochemistry and Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, Georgia 30332.

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http://dx.doi.org/10.1021/jz100248cDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2860151PMC
January 2010

Yeast display evolution of a kinetically efficient 13-amino acid substrate for lipoic acid ligase.

J Am Chem Soc 2009 Nov;131(45):16430-8

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.

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http://dx.doi.org/10.1021/ja904596fDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2799336PMC
November 2009

Fluorescent probes for super-resolution imaging in living cells.

Nat Rev Mol Cell Biol 2008 Dec 12;9(12):929-43. Epub 2008 Nov 12.

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.

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http://dx.doi.org/10.1038/nrm2531DOI Listing
December 2008

Imaging proteins in live mammalian cells with biotin ligase and monovalent streptavidin.

Nat Protoc 2008 ;3(3):534-45

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.

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http://dx.doi.org/10.1038/nprot.2008.20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2671200PMC
November 2008

An engineered aryl azide ligase for site-specific mapping of protein-protein interactions through photo-cross-linking.

Angew Chem Int Ed Engl 2008 ;47(37):7018-21

Department of Chemistry, Room 18-496, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

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http://dx.doi.org/10.1002/anie.200802088DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2745654PMC
October 2008

Site-specific modification of AAV vector particles with biophysical probes and targeting ligands using biotin ligase.

Mol Ther 2008 Aug 17;16(8):1467-73. Epub 2008 Jun 17.

Gene Therapy Center, The Research Institute at Nationwide Children's Hospital, Nationwide Children's Hospital, Columbus, Ohio, USA.

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http://linkinghub.elsevier.com/retrieve/pii/S152500161632282
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http://dx.doi.org/10.1038/mt.2008.129DOI Listing
August 2008

Protein-protein interaction detection in vitro and in cells by proximity biotinylation.

J Am Chem Soc 2008 Jul 27;130(29):9251-3. Epub 2008 Jun 27.

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.

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http://dx.doi.org/10.1021/ja801445pDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2635094PMC
July 2008

Monovalent, reduced-size quantum dots for imaging receptors on living cells.

Nat Methods 2008 May 20;5(5):397-9. Epub 2008 Apr 20.

Department of Chemistry, 77 Massachusetts Avenue, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts 02139, USA.

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http://dx.doi.org/10.1038/nmeth.1206DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2637151PMC
May 2008

Expanding the substrate tolerance of biotin ligase through exploration of enzymes from diverse species.

J Am Chem Soc 2008 Jan 3;130(4):1160-2. Epub 2008 Jan 3.

Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

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http://dx.doi.org/10.1021/ja076655iDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3501195PMC
January 2008

Compact biocompatible quantum dots functionalized for cellular imaging.

J Am Chem Soc 2008 Jan 5;130(4):1274-84. Epub 2008 Jan 5.

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139-4307, USA.

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http://dx.doi.org/10.1021/ja076069pDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2665712PMC
January 2008

Redirecting lipoic acid ligase for cell surface protein labeling with small-molecule probes.

Nat Biotechnol 2007 Dec 2;25(12):1483-7. Epub 2007 Dec 2.

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Ave., Cambridge, Massachusetts 02139, USA.

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http://dx.doi.org/10.1038/nbt1355DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2654346PMC
December 2007

Phage display evolution of a peptide substrate for yeast biotin ligase and application to two-color quantum dot labeling of cell surface proteins.

J Am Chem Soc 2007 May 2;129(20):6619-25. Epub 2007 May 2.

Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

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http://dx.doi.org/10.1021/ja071013gDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2629800PMC
May 2007

Next-generation optical technologies for illuminating genetically targeted brain circuits.

J Neurosci 2006 Oct;26(41):10380-6

Department of Bioengineering, Stanford University, Stanford, California 94305, USA.

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http://dx.doi.org/10.1523/JNEUROSCI.3863-06.2006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2820367PMC
October 2006

Synthesis of a ketone analogue of biotin via the intramolecular Pauson-Khand reaction.

Org Lett 2006 Sep;8(20):4593-5

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Room 18-496, Cambridge, Massachusetts 02139, USA.

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http://dx.doi.org/10.1021/ol061862tDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2531226PMC
September 2006

A monovalent streptavidin with a single femtomolar biotin binding site.

Nat Methods 2006 Apr;3(4):267-73

Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

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http://dx.doi.org/10.1038/nmeth861DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2576293PMC
April 2006

Transglutaminase-catalyzed site-specific conjugation of small-molecule probes to proteins in vitro and on the surface of living cells.

J Am Chem Soc 2006 Apr;128(14):4542-3

Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

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http://dx.doi.org/10.1021/ja0604111DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2561265PMC
April 2006

Giving cells a new sugar-coating.

Nat Chem Biol 2006 Mar;2(3):127-8

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http://dx.doi.org/10.1038/nchembio0306-127DOI Listing
March 2006

Targeting quantum dots to surface proteins in living cells with biotin ligase.

Proc Natl Acad Sci U S A 2005 May 16;102(21):7583-8. Epub 2005 May 16.

Department of Chemistry, The Picower Center for Learning and Memory, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

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http://dx.doi.org/10.1073/pnas.0503125102DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1129026PMC
May 2005

Site-specific labeling of proteins with small molecules in live cells.

Curr Opin Biotechnol 2005 Feb;16(1):35-40

Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

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http://dx.doi.org/10.1016/j.copbio.2004.12.003DOI Listing
February 2005

Site-specific labeling of cell surface proteins with biophysical probes using biotin ligase.

Nat Methods 2005 Feb 21;2(2):99-104. Epub 2005 Jan 21.

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Ave., Room 18-496, Cambridge, Massachusetts 02139, USA.

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http://dx.doi.org/10.1038/nmeth735DOI Listing
February 2005

Genetically encoded fluorescent reporters of histone methylation in living cells.

J Am Chem Soc 2004 May;126(19):5982-3

Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

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http://dx.doi.org/10.1021/ja038854hDOI Listing
May 2004

A genetically encoded fluorescent reporter of histone phosphorylation in living cells.

Angew Chem Int Ed Engl 2004 May;43(22):2940-3

Department of Chemistry, Room 18-496, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

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http://doi.wiley.com/10.1002/anie.200353375
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May 2004

Creating new fluorescent probes for cell biology.

Nat Rev Mol Cell Biol 2002 Dec;3(12):906-18

Department of Pharmacology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, 18-496, Cambridge, Massachusetts 02139, USA.

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http://www.nature.com/articles/nrm976
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December 2002

Signal transduction. Decoding NF-kappaB signaling.

Science 2002 Nov;298(5596):1189-90

Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

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http://dx.doi.org/10.1126/science.1079331DOI Listing
November 2002