Publications by authors named "Ali Zare-Mirzaie"

22 Publications

  • Page 1 of 1

Pre-analytical Practices in the Molecular Diagnostic Tests, A Concise Review.

Iran J Pathol 2021 10;16(1):1-19. Epub 2020 Nov 10.

Cell-based Therapies Research Center, Digestive Disease Research Institute, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.

Molecular assays for detection of nucleic acids in biologic specimens are valuable diagnostic tools supporting clinical diagnoses and therapeutic decisions. Pre-analytical errors, which occur before or during processing of nucleic acid extraction, contribute a significant role in common errors that take place in molecular laboratories. Certain practices in specimen collection, transportation, and storage can affect the integrity of nucleic acids before analysis. Applying best practices in these steps, helps to minimize those errors and leads to better decisions in patient diagnosis and treatment. Widely acceptable recommendations, which are for optimal molecular assays associated with pre-analytic variables, are limited. In this article, we have reviewed most of the important issues in sample handling from bed to bench before starting molecular tests, which can be used in diagnostic as well as research laboratories. We have addressed the most important pre-analytical points in performing molecular analysis in fixed and unfixed solid tissues, whole blood, serum, plasma, as well as most of the body fluids including urine, fecal and bronchial samples, as well as prenatal diagnosis samples.
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http://dx.doi.org/10.30699/ijp.2020.124315.2357DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7691716PMC
November 2020

Investigating Human Papilloma Virus Types in Sinonasal Papilloma Using Polymerase Chain Reaction: Is It Really a Prerequisite for Nasal Papilloma Formation?

Maedica (Bucur) 2020 Mar;15(1):76-80

Department of Pathology, Iran University of Medical Sciences, Tehran, Iran.

Sinonasal papilloma is a relatively rare disease. However, it is prevalent enough for every otorhinolaryngologist to encounter it several times throughout one's medical practice. The aim of this study was to identify the presence of Human Papilloma Virus in sinonasal specimens of patients with sinonasal papilloma. A cross sectional analytical study was performed on fresh tissue samples from 36 patients with sinonasal papilloma. Samples were studied by polymerase chain reaction for of Human Papilloma Virus detection. In conclusion, the majority of patients were of Human Papilloma Virus negative and there was no statistically significant difference in presence of squamous cell carcinoma in of Human Papilloma Virus positive and negative patients. Thus, further studies are needed to assess other potential factors that may influence the development of sinonasal papilloma.
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http://dx.doi.org/10.26574/maedica.2020.15.1.76DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7221285PMC
March 2020

MiR-21 Expression in Wilms' Tumor.

Iran J Pathol 2018 25;13(4):422-428. Epub 2018 Sep 25.

Cell Based Therapies Research Center , Digestive Disease Research Institute, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.

Background And Objective: Wilms' tumor (WT) is the most common genitourinary tract tumor in children. MicroRNAs (miRNAs) are small non-coding RNAs; their role in the pathogenesis of many types of human cancers has been identified. We aimed to evaluate the expression of miR-21, a well-known oncomir, in WT tissue samples which is a very common urinary tract malignancy in children.

Methods: We performed chromogenic in situ hybridization (CISH) to detect the sub-cellular localization of miR-21 in 25 formalin-fixed, paraffin-embedded (FFPE) samples of WT. We also evaluated miR-21 expression in 24 of these blocks and 6 normal kidneys as controls using quantitative real-time PCR technique.

Results: While our real-time PCR analysis showed miR-21 significant overexpression in 4 tumors compared to the normal kidney samples, we could not detect significant ISH signal in any of these samples.

Conclusion: Low expression of miR-21 in WT might pinpoint the weak involvement of this miRNA in the pathogenesis of this cancer.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6358566PMC
September 2018

The Correlation Between Serum Vitamin D Level and Total Antioxidant Capacity in Diabetic and Non-diabetic Subjects in Iran.

Iran J Pathol 2018 17;13(2):212-219. Epub 2018 Jul 17.

Dept of Pathology, Iran University of Medical Sciences, Rassool Akram Hospital, Tehran, Iran.

Background & Objective: Increase in intraand extracellular glucose levels can cause oxidative stress, and theprolonged imbalance between prooxidants and antioxidantscan lead to cell damage and the associated complications in patients with diabetes. Vitamin D acts as a strong antioxidant in the body and several studies emphasized on its important role to preventoxidative stress in prediabeticand diabetic subjects. The current study aimed at determining and comparingthe total antioxidant capacity (TAC) in individuals with hemoglobin A1c(HbA1c) below and above 6.5%, and its correlation with vitamin D levels.

Methods: The current cross sectional study was conducted on a total of 107patients with diabetes (HbA1c >6.5%) and 107 non-diabetic subjects (HbA1c<6.5%)referred toRassoolAkram Hospital, Tehran, Iranfrom2015 to 2016, as the sample population. The two groups were compared regarding their TAC and vitamin D serum levels and the association between vitamin D concentration and TAC was evaluated.

Results: Age and body mass index (BMI)were significantly higher in patients with diabetes,comparedwiththe serum levels of vitamin D and TAC (P<0.001 for both). Both TAC and vitamin D levels were significantly lower in the group with diabetesand a weak significant correlation was observedbetween the two factors (r=0.2, =0.003). In multivariate regression model, the duration of diabetes was also significantly associated with TAC level (beta coefficient=-0.82, <0.001).

Conclusion: The low serum levels of TAC and vitamin D in patients with diabetescould be indicative of oxidative stress in the presence of high blood glucose levels. Supplementation of vitamin D in patients with diabetes might be effective to control thenegative impacts of the disease and decreasecells' exposure to oxidative environment in prediabetes.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6339496PMC
July 2018

A presentation of ulcerative colitis after rituximab therapy in a patient with multiple sclerosis and literature review.

Mult Scler Relat Disord 2018 May 1;22:22-26. Epub 2018 Mar 1.

MS Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran; Department of Neurology, Sina Hospital, Tehran University of Medical Sciences, Tehran, Iran. Electronic address:

Multiple sclerosis (MS) is one of the most important demyelinating diseases that affects the central nervous system. Its treatment often involves a long-term disease modifying therapy. According to some studies, the prevalence of autoimmune disorders, such as autoimmune hepatitis (AIH) and ulcerative colitis (UC) is higher in MS patients than in the normal population. There are also few studies that have reported the onset of UC after rituximab therapy. The present study presents a report of a 31-years old female patient suffering from aggressive multiple sclerosis, which developed into autoimmune hepatitis during the MS therapy. Thereafter, she received rituximab for the treating both MS and AIH. One week after the third cycle of rituximab (6 doses of 1000 mg), she experienced abdominal pain, fever, and severe bloody diarrhea; finally, she was diagnosed with ulcerative colitis (UC). It seems that the administration of certain immunomodulators or immunosuppressive drugs may have a main role in the exacerbation of some autoimmune diseases.
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http://dx.doi.org/10.1016/j.msard.2018.02.030DOI Listing
May 2018

Photoclinic.

Arch Iran Med 2017 Oct;20(10):655-656

Fellow of Molecular Pathology and Cytogenetics,Pathology Department, Medical Faculty, Tehran University of Medical Sciences, Tehran, Iran.

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October 2017

Investigating The Frequency of Serrated Polyps/Adenomas and Their Subtypes in Colonic Polyp Samples.

Med Arch 2016 Jun 31;70(3):198-202. Epub 2016 May 31.

Department of Pathology, Iran University of Medical Sciences, Tehran, Iran.

Background: The purpose of this study was to determine the frequency of Serrated polyps of colonic polyps samples in Hazrat_e Rasoul_e Akram Hospital over ten years.

Materials: The target group in this study was patients with colonic polyps in Hazrat_e Rasoul_e Akram Hospital. Pathologic evaluation of these patients was done. Serrated polyps, by location, gender, age and type of polyps were divided and frequency of them were determined separately.

Results: Of 381 patients studied, 224 (58.79%) and 157(41.20%) were males and females, respectively. Mean age of patients was 59.25 years. In initial diagnosis, frequency of Adenomatous polyp, Hyperplastic polyp and Mixed polyp were 92.44% and 5.33%, and 2.22%, respectively. In final diagnosis (Second evaluation), frequency of Adenomatous polyp, Hyperplastic polyp, Mixed polyp, Sessile Serrated Adenoma/ Polyp, Traditional Serrated Adenoma and SPU (Serrated Polyp Unclassifiable) were 90.44%, 4.88%, 2.44%, 1.11%, 0.66% and 0.44%, respectively. 72.13% and 27.86% of polyps were low grade dysplasia and high grade dysplasia, respectively. According to the results of this study, the incidence of all types of polyps detected was more in men than women. Rectum and sigmoid were most abundant in the area polyp in both initial and final diagnosis.

Conclusion: Despite the low prevalence of Serrated polyps in patients, early diagnosis is the best action to reduce morbidity and mortality. Probability of the risk of progression from low grade to high grade dysplasia and transforming into Adenocarcinoma is high in Serrated polyps.
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http://dx.doi.org/10.5455/medarh.2016.70.198-202DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5010071PMC
June 2016

Fever,Weight Loss and Early Satiety due to Gastric Inflammatory Myofibroblastic Tumor; Case Report and Literature Review.

Middle East J Dig Dis 2016 Apr;8(2):138-42

Medical student, Colorectal Research center, Medical Student Research Committee (MSRC), Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.

Gastric inflammatory myofibroblastic tumor (IMT) is a rare tumor with and unpredictable prognosis usually find in young adults. We present an 18-yearold man with gastric IMT. He complained of epigastric pain, intermittent fever and night sweating associated with weight loss since two years ago. Physical examination showed anemic and cachestic features with mild abdominal tenderness in palpitation as well as an abdominal mass in epigastrium. Abdominal CT scan revealed a huge mass that was arising from the stomach. Upper endoscopy revealed a submucosal lesion in fundus of stomach of approximately 8cm. Endoscopic ultrasound showed a large sub-mucosal non homogenous fundal mass with areas of calcification. The patient underwent laparoscopic partial gastrectomy. Histopathologyand immunohistochemistryevaluation revealed an IMT.
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http://dx.doi.org/10.15171/mejdd.2016.19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4885613PMC
April 2016

Diagnostic Accuracy of Fine Needle Aspiration Cytology versus Concurrent Core Needle Biopsy in Evaluation of Intrathoracic Lesions: a Retrospective Comparative Study.

Asian Pac J Cancer Prev 2015 ;16(16):7385-90

Department of Pathology, Sina Hospital, Tehran University of Medical Sciences, Tehran, Iran E-mail :

Background: Transthoracic fine needle aspiration (FNA) cytology and core needle biopsy (CNB) are two commonly used approaches for the diagnosis of suspected neoplastic intrathoracic lesions. This study compared the diagnostic accuracy of FNA cytology and concurrent CNB in the evaluation of intrathoracic lesions.

Materials And Methods: We studied FNA cytology and concurrent CNB specimens of 127 patients retrospectively, using hematoxylin and eosin (H and E), immunohistochemistry, and, on certain occasions cytochemistry. Information regarding additional tissue tests was derived from the electronic archives of the Department of Pathology and Laboratory Medicine as well as patient records. Diagnostic accuracy was calculated for each test.

Results: Of 127 cases, 22 were inconclusive and excluded from the study. The remaining 105 were categorized into 73 (69.5%) malignant lesions and 32 (30.5%) benign lesions. FNA and CNB findings were in complete agreement in 63 cases (60%). The accuracy and confidence intervals (CIs) of FNA and CNB for malignant tumors were 86.3% (CI: 79.3-90.7) and 93.2% (CI: 87.3- 96.0 ) respectively. For epithelial malignant neoplasms, a definitive diagnosis was made in 44.8% of cases by FNA and 80.6% by CNB. The diagnostic accuracy of CNB for nonepithelial malignant neoplasms was 83.3% compared with 50% for FNA. Of the 32 benign cases, we made specific diagnoses in 16 with diagnostic accuracy of 81.3% and 6.3% for CNB and FNA, respectively.

Conclusions: Our findings suggest that FNA is comparable to CNB in the diagnosis of malignant epithelial lesions whereas diagnostic accuracy of CNB for nonepithlial malignant neoplasms is superior to that for FNA. Further, for histological typing of tumors and examining tumor origin, immunohistochemical work up plays an important role.
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http://dx.doi.org/10.7314/apjcp.2015.16.16.7385DOI Listing
September 2016

Does routine repeat testing of critical laboratory values improve their accuracy?

Med J Islam Repub Iran 2015 9;29:176. Epub 2015 Feb 9.

Professor, Iran University of Medical Sciences, Pathology Ward, Rasoul-e-Akram Hospital, Tehran, Iran.

Background: Routine repeat testing of critical laboratory values is very common these days to increase their accuracy and to avoid reporting false or infeasible results. We figure that repeat testing of critical laboratory values has any benefits or not.

Methods: We examined 2233 repeated critical laboratory values in 13 different hematology and chemistry tests including: hemoglobin, white blood cell, platelet, international normalized ratio, partial thromboplastin time, glucose, potassium, sodium, phosphorus, magnesium, calcium, total bilirubin and direct bilirubin. The absolute difference and the percentage of change between the two tests for each critical value were calculated and then compared with the College of American Pathologists/Clinical Laboratory Improvement Amendments allowable error.

Results: Repeat testing yielded results that were within the allowable error on 2213 of 2233 specimens (99.1%). There was only one outlier (0.2%) in the white blood cell test category, 9 (2.9%) in the platelet test category, 5 (4%) in the partial thromboplastin time test category, 5 (4.8%) in the international normalized ratio test category and none in other test categories.

Conclusion: Routine, repeat testing of critical hemoglobin, white blood cell, platelet, international normalized ratio, partial thromboplastin time, glucose, potassium, sodium, phosphorus, magnesium, calcium, total bilirubin and direct bilirubin results does not have any benefits to increase their accuracy.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4431443PMC
June 2015

Left sided colorectal adenomatous polyps have more risk for high grade dysplasia.

Acta Med Iran 2013 Apr 6;51(3):172-7. Epub 2013 Apr 6.

Department of Pathology, Rasoul-e-Akram Hospital, Tehran University of Medical Sciences, Tehran, Iran.

Colorectal cancer is the second leading cause of cancer death worldwide. Through risk assessment of malignancy in polyps, screening programs can achieve the best results. This study aimed to determine the association between the grade of dysplasia and the location of colorectal polyps. 240 colorectal adenomatous polyps which were referred to department of pathology at Rasoul-e-Akram Hospital between 2005 and 2009 met the inclusion criteria. Demographic data and information about size of polyps, grade of dysplasia and location of polyps were collected and analyzed by Chi-square and t-test. 124 (58.8%) patients were male and 87 (41.2%) were female. The mean age of the patients was 61.6 years. 47 (19.6%) polyps were right-sided and 193 (80.4%) were left sided. 39 (16.2%) polyps had high grade dysplasia. 27 (12.8%) patients had 56 synchronous polyps (23.3%). 176 (73.3%), 39 (16.2%) and 25 (10.5%) polyps were of tubular, tubulovillous and villous types respectively. The greatest dimension of 110 (45.8%) polyps was <0.5 cm, 97 (40.4%) 0.5-1 cm, 22 (9.2%) between 1 and 2 cm and finally 11 (4.6%) >2 cm. High grade dysplasia was detected in 3 (6.3 %) of right-sided and 36 (18.6%) of left-sided polyps. The obtained results revealed that size of polyp and amount of villous component were strongly associated with high grade dysplasia. Left side location of polyps was independently associated with high grade dysplasia. Left-sided colorectal polyps must be treated more seriously, especially the larger ones with villous component. There was no association between age and gender and the grade of dysplasia.
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April 2013

Group B streptococci urine isolates and their antimicrobial susceptibility profiles in a group of Iranian females: prevalence and seasonal variations.

Acta Clin Croat 2012 Dec;51(4):623-6

Department of Pathology, Hazrate Rasoul-e-Akram Hospital, Tehran University of Medical Sciences, Tehran, Iran.

Streptococcus agalactiae is one of the uropathogens responsible for urinary tract infections (UTI) in children, pregnant women, and elderly people with chronic underlying diseases. This study was performed to determine the prevalence of urinary tract isolates of group B streptococci (GBS) in a group of females referred to a referral University Hospital in Iran. In this retrospective cross-sectional study, urine analysis and urine culture results of all female subjects referred to the laboratory of the Rasoul-e-Akram Hospital, Tehran, Iran in 2010 were reviewed. Bacteriuria, colony count, pyuria and demographic data of patients were also evaluated. In this study, 10,256 females were investigated; 2061 (20.1%) of them had positive urine cultures. GBS was the isolated microorganism in 184 (8.92%) cases, yielding a prevalence of 1.79% in total study population. The mean age of subjects with positive GBS cultures was 48.24 +/- 18.8 years, with a higher prevalence recorded in the 51-60 and 21-30 age groups. The highest rates of cultures positive for GBS were seen in December and January. GBS was found to be sensitive to the following antibiotics: cephalothin (100%), norfloxacin (96.9%), ampicillin (96%), nitrofurantoin (95.5%), and vancomycin (95%). In this study, GBS showed greatest resistance to tetracycline (81.6%) and co-trimoxazole (68.9%). In conclusion, the prevalence of GBS in females with suspected UTI is relatively low; however, attention to the age and susceptibility pattern of antibiotic treatment for UTI caused by this microorganism is necessary.
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December 2012

The frequency of latent prostate carcinoma in autopsies of over 50 years old males, the Iranian experience.

Med J Islam Repub Iran 2012 May;26(2):73-7

MD. Assistant professor of pathology. Hazrat Rasoul-e-Akram Hospital. Tehran University of Medical Sciences. Tehran, Iran. P.O box: 14455- 364, Tel: 009821-66525587.

Background: Latent adenocarcinoma of prostate refers to cases who present no obvious sign or symptom during their life spans and the tumor is incidentally found at postmortem examination. Its frequency can be very important in epidemiologic investigations, prevention and treatment. No previous study has been done in this regard in Iran and the studies performed in other countries show various results. The aim of present study is to determine the frequency of latent prostate carcinoma by studying the corpora of men above 50 years old referred to forensic medicine organization in Tehran in 2008 and 2009.

Methods: In this study, 149 men aged above 50 who had died of different reasons and undergone autopsy were examined. Their prostates were excised and the slides were studied for the presence of adenocarcioma and prostatic intraepithelial neoplasia (PIN). In each case, age, weight of prostate, location of lesion, and grading according to Gleason's system were determined and the results were analyzed by SPSS software (version 16).

Results: Out of 149 cases, 34(22.8%) had low grade PIN, 26(17.4%) high grade PIN, and 14(9.4%) invasive adenocarcinoma. Most of the tumors were located in posterior lobe of prostate and they were more frequent in older cases (>65 years of age) and heavier prostates (p value <0.05). All invasive adenocarciomas were accompanied by PIN.

Conclusion: Worldwide studies show lower prevalence of latent carcinoma of prostate in Asian men than white European ones. Considering the absence of such studies in Iran, a larger study to compare and find out the precise rate of this kind of carcinoma, is recommended.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3587897PMC
May 2012

Platelet count and MPV, routinely measured but ignored parameters used in conjunction with the diagnosis of acute coronary syndrome: single study center in Iranian population, 2010.

Med J Islam Repub Iran 2012 Feb;26(1):17-21

Department of Pathology, Assistant Professor, Rasoul-e-Akram Hospital, Tehran University of Medical Sciences, Tehran, Iran.

Background: Myocardial infarction is the major cause of morbidity and mortality in industrialized countries. Platelet count and the mean platelet volume (MPV), a simple and reliable indicator of platelet size which correlates with platelet activation, might associate with troponin in acute chest pain.

Methods: We analyzed MPV of 851 patients who were admitted to Rasoul-e-Akram Hospital with acute chest pain during the year 2010. Two blood samples were taken from each patient within 4 hours of their arrival for routine hematology, including platelet count and MPV, and cardiac troponin T. Also, electrocardiograms of the patients were recorded. Association of MPV and platelet count with troponin was observed.

Results: The patients in troponin positive group, who had also ischemic electrocardiographic changes, had higher MPV values than non- acute coronary syndrome (ACS) patients with normal cardiac troponin T levels (9.9 vs 9.5 fl with p< 0.001). In troponin negative group, the mean of platelet count was higher than that in the positive group (221683 vs 198814/µl with p< 0.001).

Conclusion: MPV and platelet count are inexpensive laboratory tests which can be measured in association with other laboratory biomarkers in patients presenting with acute chest pain. This could help to lower hospitalization rates and also avoid misdiagnosis and having complications of patients with ACS.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3587888PMC
February 2012

Epidermodysplasia verruciformis associated with plasmablastic lymphoma and hepatitis B virus infection.

Acta Dermatovenerol Croat 2012 ;20(4):267-71

Department of Pathology, Hazrat-e-Rasoul Hospital, Teheran University of Medical Sciences, Iran.

Epidermodysplasia verruciformis is a rare genodermatosis characterized by inherited susceptibility to infection with certain papillomaviruses, which leads to the development of disseminated plane wart-like lesions. In some patients, lesions resembling pityriasis versicolor appear. Epidermodysplasia verruciformis has also been reported in immunosuppressed patients, most notably those with HIV infection. The affected patients are predisposed to development of skin and mucosal malignancies. We describe the rare occurrence of plasmablastic lymphoma in a patient with long lasting epidermodysplasia verruciformis and hepatitis B virus infection.
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December 2013

Detection of mucosal type human papillomavirus in cutaneous squamous cell carcinoma in Iran.

Pathol Res Pract 2013 Feb 6;209(2):90-4. Epub 2012 Dec 6.

Oncopathology Research Centre, Tehran University of Medical Sciences, Tehran, Iran.

Squamous cell carcinoma (SCC) is the second most common non-melanoma skin cancer. The oncogenic role of human papilloma virus (HPV) in cutaneous SCC has been suggested by several studies performed on immunosuppresed patients. However, the role of mucosal type HPV in SCC patients with normal immunity has not been studied extensively. Sixty skin biopsies from immunocompetent SCC patients and 60 benign skin specimens were evaluated for mucosal type HPV DNA using polymerase chain reaction (PCR) and immunohistochemistry (IHC). Mucosal type HPV DNA was detected in 18 of 60 cases (30%) and in 7 of 60 controls (11.6%) using PCR. HPV immunostain was positive in 16 of 60 cases (26.6%) and in 15 of 60 controls (25%). Mixed infection with HPV 18, 11, 6 was found in half of the SCC cases. The most prevalent subtype was HPV 18 followed by HPV 6 and 11. The frequency of HPV DNA was significantly elevated in our cases compared to controls (P value <0.01, OR=16.8, 95% CI: 3.3-74.9). Our findings suggest an association of mucosal type HPV, especially HPV 18, with skin SCC in Iranian patients with normal immunity.
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http://dx.doi.org/10.1016/j.prp.2012.10.010DOI Listing
February 2013

Analysis of colonic mucosa mast cell count in patients with chronic diarrhea.

Saudi J Gastroenterol 2012 Sep-Oct;18(5):322-6

Department of Pathology, Tehran University of Medical Sciences, Tehran, Iran.

Background/aim: Chronic diarrhea is defined as a decrease in fecal consistency lasting for four or more weeks. Prevalence of this complication in the general population is 5%. Mast cells that play an important role in the regulation of gastrointestinal visceral sensitivity and vascular permeability may be involved in functional chronic diarrhea. In this study we tried to evaluate mast cells density in colonic mucosa of patients with chronic diarrhea.

Patients And Methods: 50 patients with chronic diarrhea and 50 persons as control group were investigated. All specimens were immunohistochemically stained for mast cell tryptase (MCT) with monoclonal mouse anti-human MCT as well as toluidine blue. Mean number of mucosal mast cells were counted in 10 high power microscopic fields of patients and control groups.

Results: In patients group (age range, 15-78 years; 26 females), the number of mast cells per high power field in the immunohistochemistry staining was 21.3 ± 4.8 compared to 14.2 ± 3.4 in the control group (age range, 18-78 years; 24 females) [P < 0.001]. Also number of mast cells in toluidine blue staining was 10.3 ± 3.6 per high power field in cases and 7.1 ± 2.4 in the control group (P < 0.001).

Conclusion: Elevated number of colonic mast cells exist in patients with chronic diarrhea. Further research should be considered on application of these findings for new therapeutic opportunities.
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http://dx.doi.org/10.4103/1319-3767.101128DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3500021PMC
March 2013

Head and neck squamous cell carcinoma in Iranian patients and risk factors in young adults: a fifteen-year study.

Asian Pac J Cancer Prev 2012 ;13(7):3373-8

Department of Pathology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

Background: Head and neck squamous cell carcinoma (HNSCC) is the 8th most common cancer worldwide. Although older age, male gender, smoking and alcohol consumption are known risk factors, an increasing number of HNSCC patients are without typical risk factors. Our aim was to define demographics of HNSCC in Iran and the potential risk factors related to Iranian ethnicity and lifestyle.

Methods: We conducted a cross-sectional analytical study on 262 patients with primary SCC of the larynx, hypopharynx or tongue referred to our pathology department during 1995-2010. Patients' demographics, tumor characteristics and risk factors such as smoking, alcohol consumption and anemia were analyzed and compared in two groups of patients: over 40 years (older group) and 40 years or less (young group); Chi-square and Mann-Whitney analytical tests were employed.

Results: 5.7% of patients were young adults. The male to female ratio was 1.5 in the younger group and 5.6 in the older group. In young adults, 40% of tumors were located in larynx and 40% in the tongue. Age >40 was significantly associated with laryngeal location (P<0.001). History of smoking and drinking was significantly associated with age >40 and SCC of larynx in both age groups. Cervical lymph node involvement was significantly correlated with SCC of tongue (P<0.001), however, considering young adults only, SCC of hypopharynx was most frequently accompanied by lymph node involvement (60%). The most prevalent tumor among men was SCC of larynx whereas SCC of hypopharynx was the most prevalent tumor among women (61%), of whom 18.2% were ≤40.

Conclusions: The incidence of HNSCC among young adults seems to be higher in Iran compared to other countries. Reduction in exposure to known risk factors, especially tobacco smoking in forms of cigarettes and bubble pipes, and search for other causative agents of HNSCC in young population is recommended.
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http://dx.doi.org/10.7314/apjcp.2012.13.7.3373DOI Listing
June 2013

Bilateral ovarian involvement: a rare presentation of disseminated multiple myeloma.

J Obstet Gynaecol Res 2013 Jan 29;39(1):446-9. Epub 2012 Jul 29.

Departments of Pathology Radiology, Shahid Modarres Hospital, Shahid Beheshti University of Medical Sciences Department of Pathology, Hazrat Rasool Hospital, Tehran University of Medical Sciences, Tehran, Iran.

Extramedullary spread of multiple myeloma is extremely rare. This paper reports a case of bilateral ovarian involvement in plasma cell myeloma. The patient was a 48-year-old Persian woman who experienced constitutional symptoms, bone pain and flashing for one year. Her condition showed multiple myeloma with lytic lesions of the skull, plasma cell infiltration in bone marrow and positive light chain lambda type in serum. Workup showed huge bilateral ovarian masses, and ovarian metastasis from plasma cell myeloma was confirmed after pathologic assessment. Multiple myeloma spread to ovary is rare and according to our knowledge, this is the fourth reported case of bilateral ovarian involvement in extramedullary multiple myeloma.
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http://dx.doi.org/10.1111/j.1447-0756.2012.01972.xDOI Listing
January 2013

Diagnosis of endometrial nerve fibers in women with endometriosis.

Arch Gynecol Obstet 2011 Nov 17;284(5):1157-62. Epub 2010 Dec 17.

Minimally Invasive Surgery Research Center, Rassoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran.

Purpose: Recent studies indicated that there is a high density of small nerve fibers in the functional layer of the endometrium in women with endometriosis and that it can be used as a marker to detect endometriosis. In this study, the efficacy assessment of small nerve fibers' density as a diagnostic marker was compared in patients with and without endometriosis.

Methods: In this study, women with history of pelvic pain and/or infertility who were candidates for laparoscopy or laparotomy in Rassoul hospital (2007-2009) were enrolled. Histological sections of endometrial tissue were prepared from endometrial biopsy from women with endometriosis (n = 12) (1) and without endometriosis (n = 15) (2). Protein gene product 9.5 and neurofilament were evaluated as marker from endometrial biopsies by immunohistochemical methods.

Results: There was no statistically significant difference between two groups according to age, body mass index. Nerve fibers were detected in all endometrial biopsies from all women with endometriosis but detected only in three women without endometriosis. The mean density of nerve fibers was 2.2 ± 4.7 mm(-2) in group without endometriosis and) 13.1 ± 3.3 (in group with endometriosis (p < 0.001). Women with endometriosis had significantly higher nerve fiber density in comparison with women without endometriosis.

Conclusions: Our findings indicated that endometrial biopsy for detecting density of nerve fibers by usage of protein gene product 9.5, provided a reliable marker for diagnosis of endometriosis.
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http://dx.doi.org/10.1007/s00404-010-1806-5DOI Listing
November 2011

Clinicopathologic comparison of radiofrequency versus scalpel incision for upper blepharoplasty.

Ophthalmic Plast Reconstr Surg 2008 Nov-Dec;24(6):450-3

Iran University Eye Research Center and daggerDepartment of Pathology, Rassoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran.

Purpose: To compare the sensation recovery, scar formation, and histopathologic aspects of upper blepharoplasty eyelid incision performed with radiofrequency or scalpel.

Methods: In a prospective, randomized, blind, comparative, interventional study, 46 eyelids from 23 subjects underwent upper blepharoplasty using radiofrequency incision on one side and a scalpel on the other side. Exclusion criteria were diseases resulting in peripheral neuropathy such as diabetes mellitus, previous upper eyelid surgery or trauma, and history of skin disease. Eyelid sensation was measured preoperatively and 1 week, 1 month, and 6 to 7 months after surgery by a masked observer. Eyelid scar was evaluated by 2 masked observers at final follow-up. Histologic examination for zone and depth of tissue damage, hemorrhage, and inflammatory cell reaction was performed in a masked fashion on 16 eyelids.

Results: The mean patient age was 52 years (SD, 5.48 years). Mean aesthesiometry reading significantly decreased in both groups at all follow-up visits in comparison with preoperative measurement (p = 0.000). Mean sensation recovery was insignificantly (0.2 < p < 0.6) higher in the radiosurgery group at all follow-up visits. Mean scar score was insignificantly (p = 0.055 for observer 1, and 0.241 for observer 2) less in the radiosurgery group. The scar score was not significantly different between Fitzpatrick skin types 3 and 4, scored by either observer. The zone and depth of tissue damage on histopathologic examination was greater in the radiofrequency group. However, the hemorrhage and infiltration of inflammatory cells were the same.

Conclusion: There was no significant difference between radiofrequency and scalpel incision in upper blepharoplasty with regard to sensation recovery and scar formation. Histologic zone and depth of tissue damage were greater in the radiofrequency group.
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http://dx.doi.org/10.1097/IOP.0b013e31818acfceDOI Listing
March 2009