Publications by authors named "Ali Safa"

5 Publications

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Identification of Inhibitors of Integrin Cytoplasmic Domain Interactions With Syk.

Front Immunol 2020 8;11:575085. Epub 2021 Jan 8.

Molecular Cardiology Research Laboratories, Texas Heart Institute, Houston, TX, United States.

Leukocyte inflammatory responses require integrin cell-adhesion molecule signaling through spleen tyrosine kinase (Syk), a non-receptor kinase that binds directly to integrin β-chain cytoplasmic domains. Here, we developed a high-throughput screen to identify small molecule inhibitors of the Syk-integrin cytoplasmic domain interactions. Screening small molecule compound libraries identified the β-lactam antibiotics cefsulodin and ceftazidime, which inhibited integrin β-subunit cytoplasmic domain binding to the tandem SH2 domains of Syk (IC range, 1.02-4.9 µM). Modeling suggested antagonist binding to Syk outside the pITAM binding site. Ceftazidime inhibited integrin signaling Syk, including inhibition of adhesion-dependent upregulation of interleukin-1β and monocyte chemoattractant protein-1, but did not inhibit ITAM-dependent phosphorylation of Syk mediated by FcγRI signaling. Our results demonstrate a novel means to target Syk independent of its kinase and pITAM binding sites such that integrin signaling this kinase is abrogated but ITAM-dependent signaling remains intact. As integrin signaling through Syk is essential for leukocyte activation, this may represent a novel approach to target inflammation.
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http://dx.doi.org/10.3389/fimmu.2020.575085DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7819857PMC
January 2021

Prevalence of schistosomiasis and associated risk factors among school children in Um-Asher Area, Khartoum, Sudan.

BMC Res Notes 2018 Oct 31;11(1):779. Epub 2018 Oct 31.

Department of Medical Microbiology & Parasitology, School of Medical Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia.

Objective: Schistosomiasis remains one of the most common parasitic diseases worldwide. This is a cross-sectional study aimed to determine the prevalence of schistosomiasis and its associated risk factors among primary school children in Um-Asher area. The study was conducted among 170 primary school students in Um-Asher area from November 2017 to February 2018. Urine and stool samples were collected and examined for schistosomiasis infections. Moreover, data on sociodemographic characteristics and associated risk factors were obtained using a questionnaire.

Results: The overall prevalence of Schistosoma haematobium was 12.9%, whereas that of Schistosoma mansoni was 2.95%. Additionally, the males had higher prevalence (60%) of S. mansoni than females (40%). However, both gender were equally infected with S. haematobium (50%). With regard to risk factors, distance of residence from water source and source of drinking water are relatively associated with the infection.
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http://dx.doi.org/10.1186/s13104-018-3871-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6211415PMC
October 2018

Green synthesis of graphene from recycled PET bottle wastes for use in the adsorption of dyes in aqueous solution.

Ecotoxicol Environ Saf 2017 Nov 11;145:57-68. Epub 2017 Jul 11.

Fabrication Technology Research Department, Advanced Technology and New Materials Research Institute (ATNMRI), City for Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, 21934, Alexandria, Egypt. Electronic address:

Polyethyleneterephthalate (PET) is an important component of post-consumer plastic waste. This study focuses on the potential of utilizing "waste-treats-waste" by synthesis of graphene using PET bottle waste as a source material. The synthesized graphene is characterized by SEM, TEM, BET, Raman, TGA, and FT-IR. The adsorption of methylene blue (MB) and acid blue 25 (AB25) by graphene is studied and parameters such as contact time, adsorbent dosage were optimized. The Response Surface Methodology (RSM) is applied to investigate the effect of three variables (dye concentration, time and temperature) and their interaction on the removal efficiency. Adsorption kinetics and isotherm are followed a pseudo-second-order model and Langmuir and Freundlich isotherm models, respectively. Thermodynamic parameters demonstrated that adsorption of dye is spontaneous and endothermic in nature. The plastic waste can be used after transformation into valuable carbon-based nanomaterials for use in the adsorption of organic contaminants from aqueous solution.
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http://dx.doi.org/10.1016/j.ecoenv.2017.07.014DOI Listing
November 2017

Determination of celecoxib in human plasma by high-performance liquid chromatography.

J Pharm Biomed Anal 2004 May;35(3):665-70

Department of Medicinal Chemistry and Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.

A high performance liquid chromatographic method for the quantitation of celecoxib (CEL) in human plasma is presented. The method is based on liquid-liquid extraction with chloroform and reversed-phase chromatography using a Nucleosil CN column (250 mm x 4.6 mm i.d., 5 microm particle size) and UV spectrophotometer detection at 260 nm. The mobile phase consists of acetonitrile:water (60:40 (v/v)). Flutamide was used as internal standard (IS). The assay was linear in the concentration range of 10-1000 ng/ml when 0.5 ml aliquots of plasma were extracted. Within-day and between-day precision expressed by relative standard deviation is less than 4% and inaccuracy does not exceed 3%. The assay was used to analyze samples collected during human clinical studies.
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http://dx.doi.org/10.1016/j.jpba.2004.02.005DOI Listing
May 2004