Dr. Alexander Lorenz, Mag. rer. nat. Dr. rer. nat. - University of Aberdeen - Lecturer in Molecular Cell Biology

Dr. Alexander Lorenz

Mag. rer. nat. Dr. rer. nat.

University of Aberdeen

Lecturer in Molecular Cell Biology

Aberdeen | United Kingdom

Additional Specialties: Meiosis, Homologous Recombination, Schizosaccharomyces pombe

ORCID logohttps://orcid.org/0000-0003-1925-3713

Dr. Alexander Lorenz, Mag. rer. nat. Dr. rer. nat. - University of Aberdeen - Lecturer in Molecular Cell Biology

Dr. Alexander Lorenz

Mag. rer. nat. Dr. rer. nat.

Introduction

Alexander graduated in 2000 with a Mag. rer. nat. in Botany (with distinction), and in 2003 obtained a Dr. rer. nat. in Genetics (with distinction) from the University of Vienna, Austria. After working as a postdoctoral research associate with Professor Josef Loidl for two years studying the meiotic chromosome axis organization in fission yeast, he was awarded an Erwin Schrödinger-Fellowship from the Austrian Science Fund (FWF) to join Professor Matthew Whitby's lab at the Department of Biochemistry, University of Oxford. There he investigated the role of different DNA helicases in homologous recombination.

In May 2013 he joined the School of Medicine, Medical Sciences & Nutrition of the University of Aberdeen.

Primary Affiliation: University of Aberdeen - Aberdeen , United Kingdom

Additional Specialties:

Education

Apr 2003
University of Vienna, Austria
Dr. rer. nat. (Genetics)
equivalent to PhD
May 2000
University of Vienna, Austria
Mag. rer. nat. (Botany)
equivalent to MSc/MRes
Oct 1997 - Jul 1998
University of Cologne

Experience

Feb 2018
Lecturer in Molecular Cell Biology
Assistant Professor
Institute of Medical Sciences, University of Aberdeen, UK
Mar 2013
Postdoctoral Research Associate
Laboratory Researcher
Department of Biochemistry, University of Oxford, UK
Jun 2007
Erwin Schrödinger-Postdoctoral Research Fellow
Laboratory Researcher
Department of Biochemistry, University of Oxford, UK
May 2005
Postdoctoral Research Associate
Laboratory Researcher
Department of Chromosome Biology, University of Vienna, Austria
Oct 1999 - May 2003
University of Vienna
Research Assistant
Department of Cell Biology and Genetics

Publications

32Publications

74Reads

367Profile Views

449PubMed Central Citations

Immediate visualization of recombination events and chromosome segregation defects in fission yeast meiosis.

Chromosoma 2019 Feb 9. Epub 2019 Feb 9.

Institute of Medical Sciences (IMS), University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, UK.

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http://dx.doi.org/10.1007/s00412-019-00691-yDOI Listing
February 2019
4.602 Impact Factor

The mycoparasitic yeast Saccharomycopsis schoenii predates and kills multi-drug resistant Candida auris.

Sci Rep 2018 Oct 8;8(1):14959. Epub 2018 Oct 8.

Carlsberg Research Laboratory, Yeast & Fermentation, DK-1799, Copenhagen V, Denmark.

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http://www.nature.com/articles/s41598-018-33199-z
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http://dx.doi.org/10.1038/s41598-018-33199-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6175896PMC
October 2018
2 Reads
5.080 Impact Factor

Genetic interactions between the chromosome axis-associated protein Hop1 and homologous recombination determinants in Schizosaccharomyces pombe.

Curr Genet 2018 Oct 17;64(5):1089-1104. Epub 2018 Mar 17.

Institute of Medical Sciences (IMS), University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, UK.

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http://dx.doi.org/10.1007/s00294-018-0827-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6153652PMC
October 2018
5 Reads
1 Citation
2.682 Impact Factor

Modulation of meiotic homologous recombination by DNA helicases.

Authors:
Alexander Lorenz

Yeast 2017 05 27;34(5):195-203. Epub 2017 Jan 27.

Institute of Medical Sciences, School of Medicine, Medical Sciences and Nutrition, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, UK.

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http://dx.doi.org/10.1002/yea.3227DOI Listing
May 2017
2 Reads
2 Citations
1.634 Impact Factor

Single-step marker switching in Schizosaccharomyces pombe using a lithium acetate transformation protocol

Bio-Protocol 6 (24): e2075

Bio-Protocol

The ability to utilize different selectable markers for tagging or mutating multiple genes in Schizosaccharomyces pombe is hampered by the historical use of only two selectable markers, ura4+ and kanMX6; the latter conferring resistance to the antibiotic G418 (geneticin). More markers have been described recently, but introducing these into yeast cells often requires strain construction from scratch. To overcome this problem we and other groups have created transformation cassettes with flanking homologies to ura4+ and kanMX6 which enable an efficient and time-saving way to exchange markers in existing mutated or tagged fission yeast strains. Here, we present a protocol for single-step marker switching by lithium acetate transformation in fission yeast, Schizosaccharomyces pombe. In the following we describe how to swap the ura4+ marker to a kanMX6, natMX4, or hphMX4 marker, which provide resistance against the antibiotics G418, nourseothricin (clonNAT) or hygromycin B, respectively. We also detail how to exchange any of the MX markers for nutritional markers, such as arg3+, his3+, leu1+ and ura4+.

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December 2016
8 Reads

DNA double-strand break formation and repair in Tetrahymena meiosis.

Semin Cell Dev Biol 2016 06 16;54:126-34. Epub 2016 Feb 16.

Institute of Medical Sciences (IMS), University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK. Electronic address:

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http://dx.doi.org/10.1016/j.semcdb.2016.02.021DOI Listing
June 2016
2 Reads
7 Citations
6.265 Impact Factor

Meiotic chromosome mobility in fission yeast is resistant to environmental stress.

Sci Rep 2016 Apr 14;6:24222. Epub 2016 Apr 14.

Institut für Radiobiologie der Bundeswehr in Verbindung mit der Universität Ulm, Neuherbergstr. 11, D-80937 München, Germany.

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http://dx.doi.org/10.1038/srep24222DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4831013PMC
April 2016
6 Reads
5.080 Impact Factor

The RecQ DNA helicase Rqh1 constrains Exonuclease 1-dependent recombination at stalled replication forks.

Sci Rep 2016 Mar 9;6:22837. Epub 2016 Mar 9.

Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU UK.

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http://dx.doi.org/10.1038/srep22837DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4783781PMC
March 2016
2 Reads
4 Citations
5.080 Impact Factor

New cassettes for single-step drug resistance and prototrophic marker switching in fission yeast.

Authors:
Alexander Lorenz

Yeast 2015 Dec 17;32(12):703-10. Epub 2015 Sep 17.

Institute of Medical Sciences, University of Aberdeen, UK.

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http://dx.doi.org/10.1002/yea.3097DOI Listing
December 2015
1 Read
3 Citations
1.634 Impact Factor

Rad51/Dmc1 paralogs and mediators oppose DNA helicases to limit hybrid DNA formation and promote crossovers during meiotic recombination.

Nucleic Acids Res 2014 Dec 20;42(22):13723-35. Epub 2014 Nov 20.

Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK

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http://dx.doi.org/10.1093/nar/gku1219DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4267644PMC
December 2014
1 Read
3 Citations
9.112 Impact Factor

Slx8 removes Pli1-dependent protein-SUMO conjugates including SUMOylated topoisomerase I to promote genome stability.

PLoS One 2013 6;8(8):e71960. Epub 2013 Aug 6.

Department of Biochemistry, University of Oxford, Oxford, United Kingdom.

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0071960PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3735562PMC
April 2014
1 Read
4 Citations
3.234 Impact Factor

How not to get cross(ed): a novel role for FANCM orthologs in meiotic recombination.

Cell Cycle 2012 Sep 23;11(18):3347-8. Epub 2012 Aug 23.

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http://dx.doi.org/10.4161/cc.21844DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3466536PMC
September 2012
1 Read
1 Citation

The fission yeast FANCM ortholog directs non-crossover recombination during meiosis.

Science 2012 Jun;336(6088):1585-8

Department of Biochemistry, University of Oxford, Oxford, UK.

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http://dx.doi.org/10.1126/science.1220111DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3399777PMC
June 2012
2 Reads
40 Citations
31.480 Impact Factor

Mug20, a novel protein associated with linear elements in fission yeast meiosis.

Curr Genet 2012 Apr 24;58(2):119-27. Epub 2012 Feb 24.

Department of Chromosome Biology, Center for Molecular Biology of the University of Vienna (MFPL), Dr. Bohr Gasse 1, 1030 Vienna, Austria.

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http://dx.doi.org/10.1007/s00294-012-0369-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3310140PMC
April 2012
1 Read
10 Citations
2.682 Impact Factor

The DNA helicase Pfh1 promotes fork merging at replication termination sites to ensure genome stability.

Genes Dev 2012 Mar;26(6):594-602

Department of Biochemistry, University of Oxford, Oxford OX13QU, United Kingdom.

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http://dx.doi.org/10.1101/gad.184663.111DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3315120PMC
March 2012
5 Reads
29 Citations
10.800 Impact Factor

Ultrafine anaphase bridges, broken DNA and illegitimate recombination induced by a replication fork barrier.

Nucleic Acids Res 2011 Aug 16;39(15):6568-84. Epub 2011 May 16.

Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK.

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http://nar.oxfordjournals.org/content/early/2011/05/14/nar.g
Web Search
http://nar.oxfordjournals.org/lookup/doi/10.1093/nar/gkr340
Publisher Site
http://dx.doi.org/10.1093/nar/gkr340DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3159475PMC
August 2011
4 Reads
22 Citations
9.112 Impact Factor

A failure of meiotic chromosome segregation in a fbh1Delta mutant correlates with persistent Rad51-DNA associations.

Nucleic Acids Res 2011 Mar 11;39(5):1718-31. Epub 2010 Dec 11.

Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.

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http://dx.doi.org/10.1093/nar/gkq977DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3061084PMC
March 2011
3 Reads
7 Citations
9.112 Impact Factor

The human Holliday junction resolvase GEN1 rescues the meiotic phenotype of a Schizosaccharomyces pombe mus81 mutant.

Nucleic Acids Res 2010 Apr 29;38(6):1866-73. Epub 2009 Dec 29.

Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.

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http://dx.doi.org/10.1093/nar/gkp1179DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2847240PMC
April 2010
1 Read
25 Citations
9.112 Impact Factor

Roles of Hop1 and Mek1 in meiotic chromosome pairing and recombination partner choice in Schizosaccharomyces pombe.

Mol Cell Biol 2010 Apr 1;30(7):1570-81. Epub 2010 Feb 1.

Institute of Cell Biology, University of Berne, Baltzer-Strasse 4, CH-3012 Berne, Switzerland.

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http://dx.doi.org/10.1128/MCB.00919-09DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2838064PMC
April 2010
5 Reads
19 Citations
4.780 Impact Factor

Analysis of Schizosaccharomyces pombe meiosis by nuclear spreading.

Methods Mol Biol 2009 ;558:15-36

Department of Chromosome Biology, Center for Molecular Biology, University of Vienna, Vienna, Austria.

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http://dx.doi.org/10.1007/978-1-60761-103-5_2DOI Listing
October 2009
3 Reads
10 Citations

Fbh1 limits Rad51-dependent recombination at blocked replication forks.

Mol Cell Biol 2009 Sep 22;29(17):4742-56. Epub 2009 Jun 22.

Department of Biochemistry, University of Oxford, Oxford, United Kingdom.

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http://dx.doi.org/10.1128/MCB.00471-09DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2725720PMC
September 2009
2 Reads
32 Citations
4.780 Impact Factor

The FANCM ortholog Fml1 promotes recombination at stalled replication forks and limits crossing over during DNA double-strand break repair.

Mol Cell 2008 Oct;32(1):118-28

Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.

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http://dx.doi.org/10.1016/j.molcel.2008.08.024DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2581491PMC
October 2008
4 Reads
83 Citations
14.020 Impact Factor

Crossover promotion and prevention.

Authors:
A Lorenz M C Whitby

Biochem Soc Trans 2006 Aug;34(Pt 4):537-41

Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX13QU, UK.

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http://dx.doi.org/10.1042/BST0340537DOI Listing
August 2006
1 Read
5 Citations
3.194 Impact Factor

Meiotic recombination proteins localize to linear elements in Schizosaccharomyces pombe.

Chromosoma 2006 Aug 31;115(4):330-40. Epub 2006 Mar 31.

Department of Chromosome Biology, University of Vienna, A-1030, Vienna, Austria.

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http://dx.doi.org/10.1007/s00412-006-0053-9DOI Listing
August 2006
2 Reads
31 Citations
4.602 Impact Factor

Yeast nuclear envelope subdomains with distinct abilities to resist membrane expansion.

Mol Biol Cell 2006 Apr 8;17(4):1768-78. Epub 2006 Feb 8.

The Laboratory of Molecular and Cellular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.

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http://dx.doi.org/10.1091/mbc.e05-09-0839DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1415286PMC
April 2006
3 Reads
37 Citations
4.470 Impact Factor

Differential activation of M26-containing meiotic recombination hot spots in Schizosaccharomyces pombe.

Genetics 2005 May 2;170(1):95-106. Epub 2005 Mar 2.

North West Cancer Research Fund Institute, University of Wales Bangor, UK.

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http://dx.doi.org/10.1534/genetics.104.036301DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1449712PMC
May 2005
3 Reads
8 Citations

S. pombe meiotic linear elements contain proteins related to synaptonemal complex components.

J Cell Sci 2004 Jul;117(Pt 15):3343-51

Institute of Botany, University of Vienna, Rennweg 14, A-1030, Austria.

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http://dx.doi.org/10.1242/jcs.01203DOI Listing
July 2004
2 Reads
35 Citations
5.432 Impact Factor

Chromosome pairing does not contribute to nuclear architecture in vegetative yeast cells.

Eukaryot Cell 2003 Oct;2(5):856-66

Institute of Botany, University of Vienna, Vienna, Austria.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC219365PMC
October 2003
2 Reads
12 Citations

Spatial organisation and behaviour of the parental chromosome sets in the nuclei of Saccharomyces cerevisiae x S. paradoxus hybrids.

J Cell Sci 2002 Oct;115(Pt 19):3829-35

Institute of Botany, University of Vienna, Rennweg 14, A-1030 Vienna, Austria.

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October 2002
3 Reads
5 Citations
5.432 Impact Factor

Chromosome associations in budding yeast caused by integrated tandemly repeated transgenes.

J Cell Sci 2002 Mar;115(Pt 6):1213-20

Institute of Botany, University of Vienna, Rennweg 14, A-1030 Vienna, Austria.

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March 2002
2 Reads
4 Citations
5.432 Impact Factor

Origin of facultative heterochromatin in the endosperm of Gagea lutea (Liliaceae)

Protoplasma 2000 Sept; 212(3-4):217-226.

Protoplasma

Facultative heterochromatin occurs not only in certain animals in connection with sex determination but also in members of at least one plant genus, Gagea (Liliaceae s. str.), but here in the course of embryo sac development, fertilization, and endosperm formation. The present contribution intends to provide undebatable photographic and cytometric evidence, previously not available, for the events in the course of which three whole genomes in the pentaploid endosperm nuclei of Gagea lutea become heterochroma-tinized. In this plant, embryo sac formation usually follows the Fritillaria type, i.e., the embryo sac is tetrasporic, and a “1 + 3 position” of the spore nuclei is followed by a mitosis in which the three chalazal spindles fuse and two triploid nuclei are formed. A triploid chalazal polar nucleus is derived from one of these, which contributes to the pentaploid endosperm. These nuclei in the chalazal part of the embryo sac show stronger condensation compared with the micropylar ones. The pycnosis of the triploid polar nucleus is maintained and even enhanced during endosperm proliferation, while the micropylar polar nucleus and the sperm nucleus maintain their euchromatic condition. The origin of the heterochromatic masses in the endosperm nuclei from the three chalazal genomes of the central cell is unambiguously evident from the distribution of heterochromatic chromosomes in the first endosperm mitosis and the following interphase. DNA content measurements confirm a 3 ∶ 2 relationship of heterochromatic and euchromatic chromosome sets, which is usually maintained up to the cellularized endosperm. Pycnotic nuclei in the chalazal part of megagametophytes are characteristic of several embryo sac types, but only for Gagea spp. it is documented that such nuclei can take part in fertilization and endosperm formation.

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September 2000
6 Reads

Top co-authors

Josef Loidl
Josef Loidl

University of Vienna

12
Matthew C Whitby
Matthew C Whitby

University of Oxford

11
Fekret Osman
Fekret Osman

University of Oxford

9
Roland Steinacher
Roland Steinacher

University of Oxford

4
Weili Sun
Weili Sun

University of Oxford

3
Harry Scherthan
Harry Scherthan

Institute of Botany

2
Saikat Nandi
Saikat Nandi

University of Oxford

2
Jong Sook Ahn
Jong Sook Ahn

University of Oxford

2