Publications by authors named "Alexander I Bennett"

2 Publications

  • Page 1 of 1

Tunable and Reversible Substrate Stiffness Reveals a Dynamic Mechanosensitivity of Cardiomyocytes.

ACS Appl Mater Interfaces 2019 Jun 30;11(23):20603-20614. Epub 2019 May 30.

Department of Physics , Bryn Mawr College , Bryn Mawr , Pennsylvania 19010 , United States.

New directions in material applications have allowed for the fresh insight into the coordination of biophysical cues and regulators. Although the role of the mechanical microenvironment on cell responses and mechanics is often studied, most analyses only consider static environments and behavior, however, cells and tissues are themselves dynamic materials that adapt in myriad ways to alterations in their environment. Here, we introduce an approach, through the addition of magnetic inclusions into a soft poly(dimethylsiloxane) elastomer, to fabricate a substrate that can be stiffened nearly instantaneously in the presence of cells through the use of a magnetic gradient to investigate short-term cellular responses to dynamic stiffening or softening. This substrate allows us to observe time-dependent changes, such as spreading, stress fiber formation, Yes-associated protein translocation, and sarcomere organization. The identification of temporal dynamic changes on a short time scale suggests that this technology can be more broadly applied to study targeted mechanisms of diverse biologic processes, including cell division, differentiation, tissue repair, pathological adaptations, and cell-death pathways. Our method provides a unique in vitro platform for studying the dynamic cell behavior by better mimicking more complex and realistic microenvironments. This platform will be amenable to future studies aimed at elucidating the mechanisms underlying mechanical sensing and signaling that influence cellular behaviors and interactions.
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http://dx.doi.org/10.1021/acsami.9b02446DOI Listing
June 2019

Stability of High Speed 3D Printing in Liquid-Like Solids.

ACS Biomater Sci Eng 2016 Oct 31;2(10):1796-1799. Epub 2016 Aug 31.

Department of Mechanical and Aerospace Engineering, University of Florida, Gainesville, Florida 32611, United States.

Fluid instabilities limit the ability of features to hold their shape in many types of 3D printing as liquid inks solidify into written structures. By 3D printing directly into a continuum of jammed granular microgels, these instabilities are circumvented by eliminating surface tension and body forces. However, this type of 3D printing process is potentially limited by inertial instabilities if performed at high speeds where turbulence may destroy features as they are written. Here, we design and test a high-speed 3D printing experimental system to identify the instabilities that arise when an injection nozzle translates at 1 m/s. We find that the viscosity of the injected material can control the Reynold's instability, and we discover an additional, unanticipated instability near the top surface of the granular microgel medium.
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http://dx.doi.org/10.1021/acsbiomaterials.6b00184DOI Listing
October 2016
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