Alberto Mercatanti - Institute of Clinical Physiology

Alberto Mercatanti

Institute of Clinical Physiology

Italy

Alberto Mercatanti - Institute of Clinical Physiology

Alberto Mercatanti

Introduction

Primary Affiliation: Institute of Clinical Physiology - Italy

Publications

16Publications

112Reads

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341PubMed Central Citations

MiR-492 impairs the angiogenic potential of endothelial cells.

J Cell Mol Med 2013 Aug 26;17(8):1006-15. Epub 2013 Jun 26.

Laboratory of Molecular and Gene Therapy, Institute of Clinical Physiology, CNR, Pisa, Italy.

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http://dx.doi.org/10.1111/jcmm.12085DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3780533PMC
August 2013
15 Reads
9 Citations

An automated plasma protein fractionation design: high-throughput perspectives for proteomic analysis.

BMC Res Notes 2012 Nov 1;5:612. Epub 2012 Nov 1.

Institute of Clinical Physiology-CNR, Via Moruzzi 1, 56124 Pisa, Italy.

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http://dx.doi.org/10.1186/1756-0500-5-612DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3517536PMC
November 2012
2 Reads

MicroRNA 218 Mediates the Effects of Tbx5a Over-Expression on Zebrafish Heart Development

Chiavacci E, Dolfi L, Verduci L, Meghini F, Gestri G, et al. (2012) MicroRNA 218 Mediates the Effect

PLOS One

tbx5, a member of the T-box gene family, encodes one of the key transcription factors mediating vertebrate heart development. Tbx5 function in heart development appears to be exquisitely sensitive to gene dosage, since both haploinsufficiency and gene duplication generate the cardiac abnormalities associated with Holt−Oram syndrome (HOS), a highly penetrant autosomal dominant disease characterized by congenital heart defects of varying severity and upper limb malformation. It is suggested that tight integration of microRNAs and transcription factors into the cardiac genetic circuitry provides a rich and robust array of regulatory interactions to control cardiac gene expression. Based on these considerations, we performed an in silico screening to identify microRNAs embedded in genes highly sensitive to Tbx5 dosage. Among the identified microRNAs, we focused our attention on miR-218-1 that, together with its host gene, slit2, is involved in heart development. We found correlated expression of tbx5 and miR-218 during cardiomyocyte differentiation of mouse P19CL6 cells. In zebrafish embryos, we show that both Tbx5 and miR-218 dysregulation have a severe impact on heart development, affecting early heart morphogenesis. Interestingly, down-regulation of miR-218 is able to rescue the heart defects generated by tbx5 over-expression supporting the notion that miR-218 is a crucial mediator of Tbx5 in heart development and suggesting its possible involvement in the onset of heart malformations.

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November 2012
11 Reads

A computational approach to predict suitable target sites for trans-acting minimal hammerhead ribozymes.

Methods Mol Biol 2012 ;848:337-56

National Research Council, Institute of Clinical Physiology, Pisa, Italy.

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http://dx.doi.org/10.1007/978-1-61779-545-9_21DOI Listing
May 2012
2 Reads
1 Citation

MicroRNA (miRNA)-mediated interaction between leukemia/lymphoma-related factor (LRF) and alternative splicing factor/splicing factor 2 (ASF/SF2) affects mouse embryonic fibroblast senescence and apoptosis.

J Biol Chem 2010 Dec 4;285(50):39551-63. Epub 2010 Oct 4.

Laboratory of Gene and Molecular Therapy, Institute of Clinical Physiology, Consiglio Nazionale delle Ricerche, 56124 Pisa, Italy.

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http://dx.doi.org/10.1074/jbc.M110.114736DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2998123PMC
December 2010
5 Reads
21 Citations
4.573 Impact Factor

The Fuzzy Logic of MicroRNA Regulation: A Key to Control Cell Complexity.

Curr Genomics 2010 Aug;11(5):350-3

Fondazione Toscana "Gabriele Monasterio", Pisa, Italy.

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http://dx.doi.org/10.2174/138920210791616707DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2945000PMC
August 2010
8 Reads
1 Citation
2.342 Impact Factor

miR-290 acts as a physiological effector of senescence in mouse embryo fibroblasts.

Physiol Genomics 2009 Nov 1;39(3):210-8. Epub 2009 Sep 1.

Laboratory of Gene and Molecular Therapy, Institute of Clinical Physiology, Consiglio Nazionale delle Ricerche, 56124 Pisa, Italy.

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http://dx.doi.org/10.1152/physiolgenomics.00085.2009DOI Listing
November 2009
20 Reads
12 Citations
2.374 Impact Factor

Connective tissue growth factor: a crucial cytokine-mediating cardiac fibrosis in ongoing enterovirus myocarditis.

J Mol Med (Berl) 2008 Jan 11;86(1):49-60. Epub 2007 Sep 11.

Department of Molecular Pathology, Institute for Pathology, University Hospital Tübingen, Tübingen, Germany.

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http://dx.doi.org/10.1007/s00109-007-0249-3DOI Listing
January 2008
19 Reads
22 Citations
5.110 Impact Factor

A proteomic approach to the investigation of early events involved in the activation of vascular smooth muscle cells.

Cell Tissue Res 2007 Jul 4;329(1):119-28. Epub 2007 Apr 4.

Laboratory of Molecular and Gene Therapy, Clinical Physiology Institute CNR, Via Moruzzi 1, 56124 Pisa, Italy.

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http://dx.doi.org/10.1007/s00441-007-0407-5DOI Listing
July 2007
2 Reads
2 Citations
3.565 Impact Factor

A proteomic approach to the investigation of early events involved in vascular smooth muscle cell activation.

Cell Tissue Res 2007 Apr 10;328(1):185-95. Epub 2007 Jan 10.

Laboratory of Molecular and Gene Therapy, Clinical Physiology Institute CNR, Pisa, Italy.

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http://dx.doi.org/10.1007/s00441-006-0357-3DOI Listing
April 2007
7 Reads
2 Citations
3.565 Impact Factor

MicroRNAs modulate the angiogenic properties of HUVECs.

Blood 2006 Nov 18;108(9):3068-71. Epub 2006 Jul 18.

Laboratory of Gene and Molecular Therapy, Institute of Clinical Physiology, CNR, Area della Ricerca, Via Moruzzi 1, 56124 Pisa, Italy.

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http://dx.doi.org/10.1182/blood-2006-01-012369DOI Listing
November 2006
5 Reads
218 Citations
10.452 Impact Factor

The energy profiling of short interfering RNAs is highly predictive of their activity.

Oligonucleotides 2004 ;14(3):227-32

Laboratorio di Terapia Genica e Molecolare, Istituto di Fisiologia Clinica, CNR, Pisa, Italy.

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http://dx.doi.org/10.1089/oli.2004.14.227DOI Listing
August 2005
1 Read
5 Citations
3.080 Impact Factor

A method for prediction of accessible sites on an mRNA sequence for target selection of hammerhead ribozymes.

J Comput Biol 2002 ;9(4):641-53

Laboratorio di Bioterapia Molecolare, CNR, Istituto di Mutagenesi e Differenziamento, Pisa, Italy.

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http://dx.doi.org/10.1089/106652702760277363DOI Listing
April 2003
1 Read
3 Citations
1.740 Impact Factor