Albert M Berghuis

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Albert M Berghuis

Publications by authors named "Albert M Berghuis"

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67Publications

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Plasticity of aminoglycoside binding to antibiotic kinase APH(2")-Ia.

Antimicrob Agents Chemother 2018 Apr 16. Epub 2018 Apr 16.

Department of Biochemistry and Groupe de Recherche Axé sur la Structure des Protéines, McGill University

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April 2018

Effect of solvent and protein dynamics in ligand recognition and inhibition of aminoglycoside adenyltransferase 2″-Ia.

Protein Sci 2017 Sep 21;26(9):1852-1863. Epub 2017 Jul 21.

McGill University, Biochemistry, 3649 Promenade Sir William Osler Room 470, Montreal, QC H3A 0G4, Canada.

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September 2017

Structural Basis for Kinase-Mediated Macrolide Antibiotic Resistance.

Structure 2017 05 13;25(5):750-761.e5. Epub 2017 Apr 13.

Department of Biochemistry, McGill University, Montréal, QC H3G 1Y6, Canada; Department of Microbiology & Immunology, McGill University, Montréal, QC H3A 2B4, Canada; Groupe de Recherche Axé sur la Structure des Protéines, McGill University, Montréal, QC H3G 0B1, Canada. Electronic address:

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May 2017

Pharmacophore Mapping of Thienopyrimidine-Based Monophosphonate (ThP-MP) Inhibitors of the Human Farnesyl Pyrophosphate Synthase.

J Med Chem 2017 Mar 28;60(5):2119-2134. Epub 2017 Feb 28.

Department of Chemistry, McGill University , 801 Sherbrooke Street West, Montreal, QC H3A 0B8, Canada.

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March 2017

Human farnesyl pyrophosphate synthase is allosterically inhibited by its own product.

Nat Commun 2017 Jan 18;8:14132. Epub 2017 Jan 18.

Department of Biochemistry, McGill University, 3649 Promenade Sir William Osler, Montreal, Quebec, Canada H3G 0B1.

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January 2017

The role of conformational flexibility in Baeyer-Villiger monooxygenase catalysis and structure.

Biochim Biophys Acta 2016 Dec 26;1864(12):1641-1648. Epub 2016 Aug 26.

Department of Biochemistry, McGill University, 3649 Promenade Sir William Osler, Bellini Pavilion, Room 466, Montreal, Quebec H3G 0B1, Canada; Department of Microbiology & Immunology, McGill University, 3649 Promenade Sir William Osler, Bellini Pavilion, Room 466, Montreal, Quebec H3G 0B1, Canada; Groupes de recherche GRASP et PROTEO, Montreal, Quebec, Canada. Electronic address:

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December 2016

Comprehensive characterization of ligand-induced plasticity changes in a dimeric enzyme.

FEBS J 2016 08 7;283(16):3029-38. Epub 2016 Jul 7.

Department of Biochemistry, Groupe de Recherche Axé sur la Structure des Protéines, McGill University, Montreal, QC, Canada.

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August 2016

Antibiotic Binding Drives Catalytic Activation of Aminoglycoside Kinase APH(2″)-Ia.

Structure 2016 06 5;24(6):935-45. Epub 2016 May 5.

Department of Biochemistry, McGill University, Montreal, QC H3G 1Y6, Canada; Groupe de Recherche Axé sur la Structure des Protéines, McGill University, Montreal, QC H3G 0B1, Canada; Department of Microbiology and Immunology, McGill University, Montreal, QC H3A 2B4, Canada. Electronic address:

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June 2016

An Entamoeba histolytica ADP-ribosyl transferase from the diphtheria toxin family modifies the bacterial elongation factor Tu.

Mol Biochem Parasitol 2016 06 24;207(2):68-74. Epub 2016 May 24.

Departments of Biochemistry and Microbiology & Immunology, McGill University, Life Sciences Complex, Francesco Bellini Building, 3649 Promenade Sir William Osler, Room 470, Montreal, QC H3G 0B1, Canada.

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June 2016

Structural Analysis of the Tobramycin and Gentamicin Clinical Resistome Reveals Limitations for Next-generation Aminoglycoside Design.

ACS Chem Biol 2016 05 4;11(5):1339-46. Epub 2016 Mar 4.

Department of Biochemistry, McGill University , McIntyre Medical Building, 3655 Promenade Sir William Osler, Montreal, Quebec, Canada , H3G 1Y6.

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May 2016

Inhibition of outer membrane proteases of the omptin family by aprotinin.

Infect Immun 2015 Jun 30;83(6):2300-11. Epub 2015 Mar 30.

Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada Microbiome and Disease Tolerance Centre, McGill University, Montreal, Quebec, Canada Faculty of Dentistry, McGill University, Montreal, Quebec, Canada

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June 2015

Probing the molecular and structural elements of ligands binding to the active site versus an allosteric pocket of the human farnesyl pyrophosphate synthase.

Bioorg Med Chem Lett 2015 Mar 13;25(5):1117-23. Epub 2015 Jan 13.

Department of Chemistry, McGill University, 801 Sherbrooke Street West, Montreal, QC H3A 0B8, Canada; Department of Biochemistry, McGill University, 3655 Promenade Sir William Osler, Montreal, QC H3G 1Y6, Canada; Groupe de Recherche Axé sur la Structure des Protéines, McGill University, 3649 Promenade Sir William Osler, Montréal, QC H3G 0B1, Canada. Electronic address:

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March 2015

Derivatives of mesoxalic acid block translocation of HIV-1 reverse transcriptase.

J Biol Chem 2015 Jan 29;290(3):1474-84. Epub 2014 Oct 29.

From the Department of Biochemistry, McGill University, Montreal, Quebec H3G 1Y6, Canada, the Department of Microbiology and Immunology, McGill University, Montreal, Quebec H3A 2B4, Canada, the Department of Medicine, Division of Experimental Medicine, McGill University, Quebec H3A 1A3, Canada

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January 2015

Lactone-bound structures of cyclohexanone monooxygenase provide insight into the stereochemistry of catalysis.

ACS Chem Biol 2014 Dec 21;9(12):2843-51. Epub 2014 Oct 21.

Departments of †Biochemistry and ‡Microbiology & Immunology, McGill University , 3649 Promenade Sir William Osler, Bellini Pavilion, Room 466, Montreal, Quebec, Canada H3G 0B1.

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December 2014

Maintenance of native-like protein dynamics may not be required for engineering functional proteins.

Chem Biol 2014 Oct 4;21(10):1330-1340. Epub 2014 Sep 4.

PROTEO Network, Université Laval, Québec QC G1V 0A6, Canada; Département de Biochimie, Université de Montréal, Montréal QC H3T 1J4, Canada; Département de Chimie, Université de Montréal, Montréal QC H3T 1J4, Canada; Center for Green Chemistry and Catalysis (CCVC), Montréal QC H3A 0B8, Canada. Electronic address:

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October 2014

Human isoprenoid synthase enzymes as therapeutic targets.

Front Chem 2014 22;2:50. Epub 2014 Jul 22.

Department of Biochemistry, McGill University Montreal, QC, Canada ; Department of Chemistry, McGill University Montreal, QC, Canada.

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August 2014

Structure of human farnesyl pyrophosphate synthase in complex with an aminopyridine bisphosphonate and two molecules of inorganic phosphate.

Acta Crystallogr F Struct Biol Commun 2014 Mar 19;70(Pt 3):299-304. Epub 2014 Feb 19.

Department of Biochemistry, McGill University, 3655 Promenade Sir William Osler, Montreal, QC H3G 1Y6, Canada.

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March 2014

Prospects for circumventing aminoglycoside kinase mediated antibiotic resistance.

Front Cell Infect Microbiol 2013 25;3:22. Epub 2013 Jun 25.

Groupe de Recherche Axé sur la Structure des Protéines, Department of Biochemistry, McGill University Montreal, QC, Canada.

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December 2013

Thienopyrimidine bisphosphonate (ThPBP) inhibitors of the human farnesyl pyrophosphate synthase: optimization and characterization of the mode of inhibition.

J Med Chem 2013 Oct 3;56(20):7939-50. Epub 2013 Oct 3.

Department of Chemistry, McGill University , 801 Sherbrooke Street West, Montreal, Quebec, Canada H3A 0B8.

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October 2013

Structural analysis of a novel cyclohexylamine oxidase from Brevibacterium oxydans IH-35A.

PLoS One 2013 26;8(3):e60072. Epub 2013 Mar 26.

Department of Biochemistry and Groupe de Recherche Axé sur la Structure des Protéines, McGill University, Montreal, Quebec, Canada.

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September 2013

The substrate-bound crystal structure of a Baeyer-Villiger monooxygenase exhibits a Criegee-like conformation.

J Am Chem Soc 2012 May 27;134(18):7788-95. Epub 2012 Apr 27.

Department of Biochemistry, McGill University, 3649 Promenade Sir William Osler, Bellini Pavilion, Room 466, Montreal, QC, Canada H3G 0B1.

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May 2012

Small-angle X-ray scattering analysis of the bifunctional antibiotic resistance enzyme aminoglycoside (6') acetyltransferase-ie/aminoglycoside (2'') phosphotransferase-ia reveals a rigid solution structure.

Antimicrob Agents Chemother 2012 Apr 30;56(4):1899-906. Epub 2012 Jan 30.

Department of Biochemistry, Groupe de Recherche Axé sur la Structure des Protéines, McGill University, Montreal, Quebec, Canada.

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April 2012

Structural basis for dual nucleotide selectivity of aminoglycoside 2''-phosphotransferase IVa provides insight on determinants of nucleotide specificity of aminoglycoside kinases.

J Biol Chem 2012 Apr 24;287(16):13094-102. Epub 2012 Feb 24.

Department of Biochemistry, Groupe de Recherche Axé sur la Structure des Protéines, McGill University, Montreal, Quebec H3G 0B1, Canada.

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April 2012

Novel crystallization conditions for tandem variant R67 DHFR yield a wild-type crystal structure.

Acta Crystallogr Sect F Struct Biol Cryst Commun 2011 Nov 25;67(Pt 11):1316-22. Epub 2011 Oct 25.

Department of Biochemistry, McGill University, 3649 Promenade Sir William Osler, Bellini Pavilion, Room 470, Montreal, Quebec H3G 0B1, Canada.

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November 2011

Crystal structures of antibiotic-bound complexes of aminoglycoside 2''-phosphotransferase IVa highlight the diversity in substrate binding modes among aminoglycoside kinases.

Biochemistry 2011 Jul 27;50(28):6237-44. Epub 2011 Jun 27.

Department of Biochemistry, Groupe de Recherche Axé sur la Structure des Protéines, McGill University, 3649 Promenade Sir William Osler, Montreal, Quebec H3G 0B1, Canada.

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July 2011

Crystal structures of two aminoglycoside kinases bound with a eukaryotic protein kinase inhibitor.

PLoS One 2011 May 9;6(5):e19589. Epub 2011 May 9.

Department of Biochemistry, McGill University, Montreal, Quebec, Canada.

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May 2011

Competing allosteric mechanisms modulate substrate binding in a dimeric enzyme.

Nat Struct Mol Biol 2011 Mar 30;18(3):288-94. Epub 2011 Jan 30.

Department of Chemistry, McGill University, Montréal, Québec, Canada.

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March 2011

Genetic analysis of B55alpha/Cdc55 protein phosphatase 2A subunits: association with the adenovirus E4orf4 protein.

J Virol 2011 Jan 3;85(1):286-95. Epub 2010 Nov 3.

Key Laboratory of Molecular Biology of Shann'xi Agriculture, College of Animal Science and Technology, Northwest A&F University, 22 XiNong Road, YangLing, Shaan'xi, People's Republic of China.

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January 2011

Preparation and characterization of bacterial protein complexes for structural analysis.

Adv Protein Chem Struct Biol 2009 27;76:1-42. Epub 2009 Nov 27.

Biotechnology Research Institute, National Research Council Canada, Montreal, Quebec, Canada.

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November 2010

Protein tyrosine phosphatases are regulated by mononuclear iron dicitrate.

J Biol Chem 2010 Aug 2;285(32):24620-8. Epub 2010 Jun 2.

Department of Microbiology and Immunology, McGill University, Montréal, Québec H3A 2B4, Canada.

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August 2010

Structure of the antibiotic resistance factor spectinomycin phosphotransferase from Legionella pneumophila.

J Biol Chem 2010 Mar 19;285(13):9545-55. Epub 2010 Jan 19.

Department of Biochemistry, McGill University, Montreal, Quebec, Canada.

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March 2010

Expression and purification of recombinant M-Pol I from Saccharomyces cerevisiae with alpha-1,6 mannosylpolymerase activity.

Protein Expr Purif 2009 Jul 26;66(1):1-6. Epub 2009 Feb 26.

McGill Cancer Centre, McGill University, Montreal, Quebec, Canada.

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July 2009

Crystal structures of cyclohexanone monooxygenase reveal complex domain movements and a sliding cofactor.

J Am Chem Soc 2009 Jul;131(25):8848-54

Department of Biochemistry, McGill University, 3649 Prom Sir William Osler, Bellini Pavilion, Room 466, Montreal, QC, Canada H3G 0B1.

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July 2009

Structural basis of APH(3')-IIIa-mediated resistance to N1-substituted aminoglycoside antibiotics.

Antimicrob Agents Chemother 2009 Jul 11;53(7):3049-55. Epub 2009 May 11.

Department of Biochemistry, McGill University, 3649 Promenade Sir William Osler, Montreal, Quebec, Canada.

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July 2009

The type IA topoisomerase catalytic cycle: A normal mode analysis and molecular dynamics simulation.

Proteins 2008 Jun;71(4):1984-94

Department of Biochemistry, McGill University, Montreal, Quebec, Canada H3A 1A4.

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June 2008

Flagellin glycosylation in Pseudomonas aeruginosa PAK requires the O-antigen biosynthesis enzyme WbpO.

J Biol Chem 2008 Feb 7;283(6):3507-18. Epub 2007 Dec 7.

Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada.

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February 2008

Structural basis for streptogramin B resistance in Staphylococcus aureus by virginiamycin B lyase.

Proc Natl Acad Sci U S A 2007 Jun 11;104(25):10388-93. Epub 2007 Jun 11.

Department of Biochemistry, McGill University, Montreal, QC, Canada H3A 4A2.

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June 2007

Identification and characterization of a protein-tyrosine phosphatase in Leishmania: Involvement in virulence.

J Biol Chem 2006 Nov 27;281(47):36257-68. Epub 2006 Sep 27.

Department of Microbiology & Immunology, Mcgill University, Montreal, Quebec, Canada.

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November 2006

Structural studies of FlaA1 from Helicobacter pylori reveal the mechanism for inverting 4,6-dehydratase activity.

J Biol Chem 2006 Aug 1;281(34):24489-95. Epub 2006 May 1.

Department of Biochemistry and Department of Microbiology and Immunology, McGill University, Montreal, Quebec.

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August 2006

Synthesis and structure-activity relationships of truncated bisubstrate inhibitors of aminoglycoside 6'-N-acetyltransferases.

J Med Chem 2006 Aug;49(17):5273-81

Department of Chemistry, McGill University, 801 Sherbrooke Street West, Montréal, Québec H3A 2K6, Canada.

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August 2006

Crystal structure of CTP:glycerol-3-phosphate cytidylyltransferase from Staphylococcus aureus: examination of structural basis for kinetic mechanism.

Biochim Biophys Acta 2006 Jan 10;1764(1):63-9. Epub 2005 Nov 10.

Department of Biochemistry, McGill University, 3655 Promenade Sir William Osler, Montreal, Quebec, Canada H3G 1Y6.

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January 2006

Crystal structure of homoserine transacetylase from Haemophilus influenzae reveals a new family of alpha/beta-hydrolases.

Biochemistry 2005 Dec;44(48):15768-73

Department of Biochemistry, McGill University, Montreal, Quebec, Canada H3A 1A4.

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December 2005

Magnesium and phosphate ions enable NAD binding to methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase.

J Biol Chem 2005 Oct 11;280(40):34316-23. Epub 2005 Aug 11.

Department of Biochemistry, McGill University, Montreal, Canada.

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October 2005

Regio- and chemoselective 6'-N-derivatization of aminoglycosides: bisubstrate inhibitors as probes to study aminoglycoside 6'-N-acetyltransferases.

Angew Chem Int Ed Engl 2005 Oct;44(42):6859-62

Department of Chemistry, McGill University, 801 Sherbrooke Street West, Montréal, Québec, H3A 2K6, Canada.

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October 2005

Structures of aminoglycoside acetyltransferase AAC(6')-Ii in a novel crystal form: structural and normal-mode analyses.

Acta Crystallogr D Biol Crystallogr 2005 Sep 16;61(Pt 9):1273-9. Epub 2005 Aug 16.

Department of Biochemistry, McGill University, 740 Dr Penfield Avenue, Montreal, QC, H3A 1A4, Canada.

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September 2005

Towards a better understanding of the substrate specificity of the UDP-N-acetylglucosamine C4 epimerase WbpP.

Biochem J 2005 Jul;389(Pt 1):173-80

Department of Microbiology and Immunology, University of Western Ontario, London, ON, N6A 5C1, Canada.

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July 2005

Crystallization and preliminary crystallographic analysis of an aminoglycoside kinase from Legionella pneumophila.

Acta Crystallogr Sect F Struct Biol Cryst Commun 2005 Jun 1;61(Pt 6):606-8. Epub 2005 Jun 1.

Department of Microbiology and Immunology, McGill University, Canada.

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June 2005

A single bifunctional UDP-GlcNAc/Glc 4-epimerase supports the synthesis of three cell surface glycoconjugates in Campylobacter jejuni.

J Biol Chem 2005 Feb 27;280(6):4792-802. Epub 2004 Oct 27.

Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario K1A 0R6, Canada.

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February 2005

Crystallization and preliminary crystallographic analysis of 3'-aminoglycoside kinase type IIIa complexed with a eukaryotic protein kinase inhibitor, CKI-7.

Acta Crystallogr D Biol Crystallogr 2004 Oct 23;60(Pt 10):1897-9. Epub 2004 Sep 23.

Department of Biochemistry, McGill University, 740 Dr Penfield Avenue, Montreal, Quebec H3A 1A4, Canada.

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October 2004

New phenolic inhibitors of yeast homoserine dehydrogenase.

Bioorg Med Chem 2004 Jul;12(14):3825-30

Antimicrobial Research Centre, Department of Biochemistry, McMaster University, 1200 Main Street West, Hamilton, ON, Canada L8N 3Z5.

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July 2004

Crystal structure of WbpP, a genuine UDP-N-acetylglucosamine 4-epimerase from Pseudomonas aeruginosa: substrate specificity in udp-hexose 4-epimerases.

J Biol Chem 2004 May 10;279(21):22635-42. Epub 2004 Mar 10.

Department of Biochemistry, McGill University, Montreal, Quebec H3A 1A4, Canada.

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May 2004

Crystal structure of WbpP, a genuine UDP-N-acetylglucosamine 4-epimerase from Pseudomonas aeruginosa: substrate specificity in udp-hexose 4-epimerases.

J Biol Chem 2004 May 10;279(21):22635-42. Epub 2004 Mar 10.

Department of Biochemistry, McGill University, Montreal, Quebec H3A 1A4, Canada.

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May 2004

X-ray structure of the AAC(6')-Ii antibiotic resistance enzyme at 1.8 A resolution; examination of oligomeric arrangements in GNAT superfamily members.

Protein Sci 2003 Mar;12(3):426-37

Departments of Biochemistry and Microbiology and Immunology, McGill University, Montreal, Quebec H3A 2B4, Canada.

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March 2003

Protein kinase inhibitors and antibiotic resistance.

Pharmacol Ther 2002 Feb-Mar;93(2-3):283-92

Department of Biochemistry, McGill University, 3775 University St., Room 613, H3A 2B4, Montreal, Quebec, Canada.

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January 2003

YjeQ, an essential, conserved, uncharacterized protein from Escherichia coli, is an unusual GTPase with circularly permuted G-motifs and marked burst kinetics.

Biochemistry 2002 Sep;41(37):11109-17

Antimicrobial Research Centre, Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada L8N 3Z5.

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September 2002

Mechanism of aminoglycoside antibiotic kinase APH(3')-IIIa: role of the nucleotide positioning loop.

Biochemistry 2002 Jun;41(22):7001-7

Department of Biochemistry, Antimicrobial Research Centre, McMaster University, Hamilton, Ontario, Canada L8N 3Z5.

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June 2002

Substrate promiscuity of an aminoglycoside antibiotic resistance enzyme via target mimicry.

EMBO J 2002 May;21(10):2323-31

Department of Biochemistry, McGill University, 3775 University Street, Montreal, Quebec H3A 2B4, Canada.

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May 2002