Akin Kirbas - Bozok University - Assoc. Prof.

Akin Kirbas

Bozok University

Assoc. Prof.

Yozgat | Turkey

Main Specialties: Gastroenterology, Hematology, Infectious Disease, Nephrology, Pulmonary Disease, Urology

Additional Specialties: Veterinary Internal Medicine

ORCID logohttps://orcid.org/0000-0001-9159-3240


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Akin Kirbas - Bozok University - Assoc. Prof.

Akin Kirbas

Introduction

He was born on 23 December 1977 in the town of Alaçam in Samsun. He completed his high school education in Alaçam High School. He has been accepted to Firat University Veterinary Faculty in 1996. In 2001, he graduated from the same faculty as the second semester. In the spring of 2003, he started his doctorate education at F?rat University, Institute of Health Sciences, Veterinary Internal Diseases Program. On December 31, 2003, he was appointed as a research assistant to the Department of Internal Medicine, Faculty of Veterinary Medicine, F?rat University. On December 31, 2007, the Ministry of Food, Agriculture and Livestock was appointed as a veterinary surgeon to the Directorate of Food, Agriculture and Livestock. After completing his doctoral studies on 23 February 2009, He was appointed as Assistant Professor in the Department of Internal Diseases, Faculty of Veterinary Medicine, Atatürk University on 16 August 2010. He received the title of Associate Professor on April 19, 2018. On October 31, 2018, he was appointed as Associate Professor in the Department of Internal Medicine, Faculty of Veterinary Medicine in Atatürk University. There are 12 Scientific Research Projects (BAP), three of which are executives. SCI and SCI Exp indexes have 21 scientific articles, 4 scientific articles in international indexes and 12 scientific articles in national journals. There are also 26 international and 21 national congresses and symposiums. He is the author of two translation books and five chapters, and is a member of the editorial board of the journal scanned at seven international indexes. He is married and has one child. He has a good command of English.

Primary Affiliation: Bozok University - Yozgat , Turkey

Specialties:

Additional Specialties:

Research Interests:


View Akin Kirbas’s Resume / CV

Education

Feb 2009
Firat University, Faculty of Veterinary Medicine, Department of Internal Medicine
PhD
Jun 2001
Firat University, Faculty of Veterinary Medicine
DVM

Publications

22Publications

261Reads

230Profile Views

130PubMed Central Citations

THE USE OF INFLAMMATORY MARKERS AS A DIAGNOSTIC AND PROGNOSTIC APPROACH IN NEONATAL CALVES WITH SEPTICAEMIA

Acta Veterinaria Hungarica

 The objective of this study was to evaluate the usefulness of inflammatory

markers as a diagnostic and prognostic approach in neonatal calves with septicaemia.

The study material consisted of 13 neonatal calves with septicaemia (septicaemic

calves, SC) and ten healthy neonatal calves (control calves, CC). Blood

samples were collected for biochemical, haematological and microbiological

analyses. In addition, faecal samples were collected for microbiological and virological

analyses. Three of neonatal calves with septicaemia were positive for E.

coli  (E. coli  O157 serotype) by microbiological examination, but all neonatal

calves with septicaemia were negative for rota- and coronaviruses. By haematological

examination, there were no significant differences between SC and CC for

white blood cell (WBC) and neutrophil (NEU) counts (P > 0.05). NEU counts

were higher on day 0 than on day 15 in SC (P < 0.05). Red blood cell (RBC)

counts and packed cell volume (PCV) values were higher on day 0 in the SC than

in the CC (P < 0.05). By biochemical analyses, tumour necrosis factor-alpha

(TNF-α ), interleukin-6 (IL-6), procalcitonin (PCT), haptoglobin (Hp), and fibrinogen

(Fb) concentrations were higher on day 0 in the SC than in the CC (P <

0.05). After treatment (on day 15), the serum IL-6, PCT, Hp, and Fb concentrations

were significantly decreased in the SC compared to the CC (P < 0.05). The

serum iron (Fe) concentrations were lower on day 0 in the SC than in the CC (P <

0.05), and were higher on day 15 than on day 0 in the SC (P < 0.05). The study

revealed that inflammatory markers could be used for determining the diagnosis

and prognosis in neonatal calves with septicaemia.

View Article
September 2019

Impact Factor 1.059

4 Reads

Investigation of the effects of hesperidin and chrysin on renal injury induced by colistin in rats.

Biomed Pharmacother 2018 Dec 10;108:1607-1616. Epub 2018 Oct 10.

Ankara University, Faculty of Veterinary Medicine, Department of Pharmacology and Toxicology, Ankara, Turkey.

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https://linkinghub.elsevier.com/retrieve/pii/S07533322183437
Publisher Site
http://dx.doi.org/10.1016/j.biopha.2018.10.001DOI Listing
December 2018
25 Reads
2.023 Impact Factor

Serological evidence of canine influenza virus infection in shelter dogs in Turkey

Med. Weter.

Influenza virus infection is an important disease which occurs in humans and a variety of animals. Because

of the wide host adaptation and segmented genome, there is always the possibility of mutations and interspecies

transmission of the influenza virus. Our study is the first to draw attention to canine influenza infection in

Turkey. For this purpose, 208 sera and swab samples were collected from dogs with respiratory and nonrespiratory

signs in various seasons. Out of the 208 dogs, 94 (45.2%) were male and 114 (54.8%) were female;

the average age was 4.7 years. A total of 208 sera samples were tested for the presence of canine influenza virusspecific

antibodies by the indirect enzyme-linked immunosorbent assay. The seroprevalence of canine influenza

virus infection was 11/208 (5.8%). With regard to seasonal distribution, the highest rate of seropositivity was

detected in spring, and the lowest in summer. Molecular detection of the canine influenza virus from nasal swab

samples was done by reverse transcriptase polymerase chain reaction using specific universal primers for the

hemagglutinin gene. Influenza virus nucleic acid could not be detected by reverse transcriptase polymerase

chain reaction. In this study, we revealed for the first time the existence of the canine influenza virus in Turkey.

Although the seroprevalence was relatively low, it would be useful to investigate the canine influenza virus on

a large scale and among dogs with infectious respiratory disease in the Turkish dog population

View Article
December 2018

Impact Factor 0.197

1 Read

Evaluation of arginase activity, nitric oxide and oxidative stress status in sheep with contagious agalactia.

Acta Vet Hung 2017 09;65(3):394-401

3 Department of Animal Science, Faculty of Veterinary Medicine, Ataturk University , 25170 Erzurum , Turkey.

It is known that inflammatory organ damages due to various agents, such as microorganisms including mycoplasmas, lead to oxidative stress. Nitric oxide (NO) functions as an antimicrobial agent, and arginase decreases proinflammatory cytokine release. There are very few studies on arginase activity, NO level and oxidative stress status in mycoplasmal infections. Therefore, the aim of this study was to evaluate erythrocyte arginase activity, plasma NO level and oxidative stress status in sheep with contagious agalactia. The study material consisted of 10 healthy sheep and 14 sheep with contagious agalactia characterised by mastitis, arthritis and keratoconjunctivitis. Erythrocyte arginase activity, plasma NO, malondialdehyde (MDA), total oxidant capacity (TOC) and total antioxidant capacity (TAC) levels were measured. Significant decreases in erythrocyte arginase activity and plasma TAC level (P < 0.001), and significant increases in plasma NO, MDA and TOC levels (P < 0.001) were found in the diseased sheep as compared with the healthy animals. This study suggests that contagious agalactia may cause oxidative stress due to increased plasma MDA and TOC levels and decreased plasma TAC levels, and that the decrease in erythrocyte arginase activity and increase in plasma NO level may contribute to the elimination of mycoplasmal agents causing contagious agalactia.

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http://dx.doi.org/10.1556/004.2017.037DOI Listing
September 2017
5 Reads
1.042 Impact Factor

Evaluation of Oxidative Stress in Sheep Infected with using Total Antioxidant Capacity, Total Oxidant Status, and Malondialdehyde Level.

J Vet Res 2017 Jun 6;61(2):197-201. Epub 2017 Dec 6.

Food and Livestock Applied Research Center, Atatürk University, 25240, Erzurum, Turkey.

Introduction: The study aimed at evaluating oxidative stress using malondialdehyde (MDA), total antioxidant capacity (TAC), total oxidant status (TOS), and oxidative stress index (OSI) markers in sheep naturally infected with (Acari).

Material And Methods: The study was performed on 40 sheep divided into two equal groups: a healthy group (group I) and a group naturally infected with (group II). The sera were obtained by centrifuging blood samples collected from the vena jugularis and serum MDA level changes in the samples were measured spectrophotometrically. Commercially available test kits were used for the measurement of TAC and TOS levels. The percentage ratio of TOS level to TAC level was accepted as OSI.

Results: The serum malondialdehyde, total oxidant status levels, and oxidative stress index increased significantly (P < 0.01) in group II, while the serum total antioxidant capacity levels decreased significantly (P < 0.01) in this group. Negative correlations between total antioxidant capacity and total oxidant status and total antioxidant capacity and malondialdehyde, and a positive correlation between total oxidant status and malondialdehyde were found in infected sheep.

Conclusion: The obtained results indicated the relationship between oxidant/antioxidant imbalance and infection in sheep. Their MDA, TAC, TOS, and OSI markers may be used to determine the oxidative stress in natural infections with .

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http://dx.doi.org/10.1515/jvetres-2017-0025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5894390PMC
June 2017
2 Reads
3 Citations
0.811 Impact Factor

Evaluation of the contraceptive eff ects of carprofen, fl unixin meglumine and meloxicam in rats

Veterinarni Medicina,

The objective of this study was to determine the suitability of carprofen, flunixin meglumine and

meloxicam for use in emergency contraception. Forty-eight pregnant Sprague-Dawley rats were used as material.

Five groups were subjected to treatments while one group served as a control. The numbers of animals in each

group were equal (n = 8). Treatment groups were administered carprofen (10 mg/kg, single or double dose, s.c.),

flunixin meglumine (5 mg/kg, single or double dose, i.m.) and meloxicam (2 mg/kg, a single dose, s.c.) on the

third day after mating. The control group received saline. The rats were sacrificed on Day 7 of gestation. Luteal

spots and implantation sites were recorded. Pre-implantation loss was calculated by subtracting the number of

luteal spots from the number of implantation sites. Compared with the control, the administration of flunixin

meglumine (double dose), carprofen (double dose) and meloxicam highly significantly decreased the implantation

rate (P < 0.001). Single dose administration of flunixin meglumine and carprofen led to significant decreases (P <

0.01). In conclusion, this study indicates that carprofen, flunixin meglumine and meloxicam treatment cause a

decline in implantation rate in rats.

View Article
May 2017

Impact Factor 0.450

2 Reads

Protective Effects of Rutin on Acute Lung Injury Induced by Oleic Acid in Rats

1

Kafkas Univ Vet Fak Derg

The purpose of this study was to explore the protective effects of different doses of rutin with the antioxidant and anti-inflammatory properties

on acute lung injury (ALI) induced by oleic acid (OA) in rats. Thirty-five Sprague-Dawley male rats were randomly separated into five groups

comprising control, rutin 150 mg, OA, rutin 75 mg + OA and rutin 150 mg + OA. In the rutin 75 mg + OA and rutin 150 mg + OA groups, the

lung malondialdehyde level (MDA) was significantly lower than that of the OA group. In the rutin 75 mg + OA and and rutin 150 mg + OA

groups, the lung GPx (glutathione peroxidase), CAT (catalase) and SOD (superoxide dismutase) activities and GSH (glutathione) levels were

significantly higher than those of the OA group, and significantly lower than those of the control group. iNOS expressions in the interstitial

parts of the lungs were significantly lower than those of the OA group. The iNOS expression was lower in the 150 mg + OA group compared

to the rutin 75 mg + OA group. It was concluded that on the ALI induced by OA, rutin had protective effects through the antioxidant and antiinflammatory

properties and that the treatment of rutin as a supportive treatment in ALI was found to be practically useful.

View Article
April 2017

1 Citation

Impact Factor 0.450

4 Reads

Effects of antioxidant vitamins (A, D, E) and trace elements (Cu, Mn, Se, Zn) on some metabolic and reproductive profiles in dairy cows during transition period

10

Polish Journal of Veterinary Sciences

The objective of this study was to determine the effects of some antioxidant vitamins and trace

elements on some metabolic and postpartum reproductive profiles in dairy cows during transition

period. In the study, altogether 20 clinically healthy Brown Swiss dairy cows (aged 4-5 years-old)

under the same management and feeding conditions in periparturient period were used. The animals

were divided into two equal groups: control (C) and treatment (T) group (n=10 for each group).

Vitamins (A, D, E) and trace elements (Cu, Mn, Se, Zn) were administered intramuscularly into the

cows of the T group, while isotonic saline, as placebo, was injected subcutaneously into those in the

C group. Blood samples were collected by venipuncture of the jugular vein at the beginning of

transition period, parturition and 3-weeks after the parturition. The metabolic and reproductive

parameters were determined. In the C group, statistically significant changes were observed in the

levels of non-esterified fatty acids (NEFA), high density lipoprotein (HDL), low density lipoprotein

(LDL), total protein (TP) (p<0.05), glucose (GLU), progesterone (P4) (p<0.01), total cholesterol

(T.CHOL), triglycerides (TG), UREA, creatinine (CRSC) and total bilirubin (TBIL) (p<0.001). In

the T group, significant changes in the levels of NEFA, TBIL (p<0.05), T.CHOL, HDL, LDL

(p<0.01), TG, GLU, P4, TAC and TOC (p<0.001) were observed. It was concluded that the administration

of various vitamins and trace elements could be effective to improve some metabolic and

reproductive profiles in dairy cows during the transition period.

View Article
December 2016

10 Citations

Impact Factor 0.830

2 Reads

CARDIAC TROPONIN-I CONCENTRATION IN WEANED CALVES WITH BOVINE RESPIRATORY DISEASE

3

Acta Veterinaria-Beograd

 The aim of this study was to evaluate the serum cardiac troponin I (cTnI) concentrations

in weaned calves with moderate and severe bovine respiratory disease. Eighteen healthy

weaned calves (group I), 18 weaned calves with moderate bovine respiratory disease

(group II) and 18 weaned calves with severe bovine respiratory disease (group III)

were included in the study. Clinical signs and severity of respiratory disease according

to clinical index scores were determined. Blood cell counts and cardiac biomarkers,

such as serum cTnI concentrations and creatine kinase-myocardial band (CK-MB), and

lactate dehydrogenase (LDH) activities were determined in all groups. Temperature

and respiratory and pulse rates were signifi  cantly increased in calves with moderate and

severe bovine respiratory disease compared to healthy calves (P < 0.05). Serum cTnI

concentrations and LDH activities were signifi  cantly increased in the calves with severe

bovine respiratory disease compared to healthy calves and calves with moderate bovine

respiratory disease (P < 0.05). A signifi  cant increase in white blood cell counts in calves

with moderate and severe bovine respiratory disease and lymphopenia and neutrophilia

in calves with severe bovine respiratory disease were also found. The study suggests

that severe bovine respiratory disease causes increased serum cTnI concentrations in

weaned calves.

View Article
December 2015

3 Citations

Impact Factor 0.670

3 Reads

Myocarditis associated with foot-and-mouth disease in suckling calves

4

VETERINARSKI ARHIV

Foot-and-mouth disease (FMD) can lead to myocarditis in young animals, but the age distributions of

calves with myocarditis have not been described, nor the biochemical profi le in these calves. In an area endemic

with foot-and-mouth disease, calves less than 6 months of age in infected farms were examined for clinical

lesions and abnormalities in respiratory rate, heart rate and heart rhythm. In total, 53 calves were identifi ed to be

suspected of having foot-and-mouth disease infection. In 6 calves myocarditis was suspected based on tachypnea,

tachycardia and gallop rhythm. In these 6 calves, cardiac troponin-I (cTnI) and aspartate aminotransferase

(AST) were signifi cantly higher (P<0.0001), but the levels of Creatinine Kinase MB (CK-MB) and Lactate

dehydrogenase (LDH) were not. These 6 calves died within 2 days and histopathology confi rmed myocarditis.

All calves with myocarditis were younger than 2-months old, suggesting that myocarditis caused by FMD is

mainly found in very young suckling calves.

View Article
July 2015

4 Citations

Impact Factor 0.320

5 Reads

Arginase activity and total oxidant/antioxidant capacity in cows with lung cystic echinococcosis

3

Med. Weter.

The aim of this study was to investigate arginase activity, total oxidant capacity (TOC), total antioxidant

capacity (TAC), and oxidative stress index (OSI) in cows with lung cystic echinococcosis (CE). The subjects

were 20 cows with lung CE (parasitized group) and 20 healthy cows (control group). A significant increase

in arginase activity, TOC, and OSI, and a significant decrease in TAC were found in the parasitized group

compared with the control group (P < 0.001). Histopathological examination revealed a fibrous tissue reaction

with inflammatory cell infiltration surrounding the CE in the lungs. The study revealed that increased arginase

activity, TOC, and OSI levels and decreased TAC levels occurred in lung CE in cows. Increased arginase activity

may be an important parameter for pulmonary fibrosis associated with lung CE in cows. Oxidant production

due to chronic inflammation, as evidenced by the increased OSI levels, might contribute to persistent lung injury

View Article
June 2015

3 Citations

Impact Factor 0.197

3 Reads

Acute Phase Biomarkers for Inflammatory Response in Dairy Cows with Traumatic Reticuloperitonitis

6

Israel Journal of Veterinary Medicine

The traumatic reticuloperitonitis (TRP) model was used to investigate the acute phase biomarkers (APBs)

in the inflammatory response of dairy cows. Fourteen Swiss Brown cows were diagnosed with TRP based

on the clinical findings, ferroscopy and ultrasonography as well as positive responses to pain tests. Four of

the cows were necropsied and TRP was confirmed. Additionally, 10 healthy cows were used as the control

group. Blood samples were obtained from cows during the clinical stage of TRP. Mean serum haptoglobulin

(Hp) (1.19±0.37 vs. 0.03±0.01 mg/mL) (P<0.05) and plasma fibrinogen (Fb) (205.1±18.1 vs. 101.1±17.6 ƞg/

mL) (P<0.001) concentrations of TRP group were found higher compared to control group. There was an

insignificant increase in mean serum amyloid A (SAA) (165±63 vs. 67.9±34 μg/mL) and α-1 acid glycoprotein

(α-1 AGP) (1069±220 vs. 663±121 μg/mL) levels (P>0.05). Mean total white blood cell (WBC) count of

TRP group (10.8±1.4 vs. 6.9±0.6 x103/μL) was significantly higher compared to control group (P<0.001).

Moreover, neutrophil counts of the TRP group showed a tendency to increase compared to the control group,

however no statistical difference was detected (P>0.05). While positive correlation was detected between

WBC count and Hp concentration of TRP group (r=0.636; P=0.01); there was no correlation between WBC

count and Fb concentration (r=0.395; P>0.05). There was no statistical difference (P>0.05) between groups

for routine biochemical parameters. In conclusion, significant increases in Fb and Hp values were found to

be related to the inflammatory response of dairy cows with TRP. The tendency of increase in the SAA and

α-1 AGP were evaluated as nonspecific for the response. In addition, high Hp values were consistent with

the correlation of high WBC counts due to the inflammatory response

View Article
June 2015

6 Citations

Impact Factor 0.300

5 Reads

Total oxidant and antioxidant capacities, nitric oxide and malondialdehyde levels in cats seropositive for the feline coronavirus

6

Veterinarni Medicina

p.p1 {margin: 0.0px 0.0px 0.0px 0.0px; font: 12.0px Times; min-height: 14.0px} p.p2 {margin: 18.0px 0.0px 0.0px 0.0px; text-align: justify; line-height: 9.6px; font: 9.5px Times} span.s1 {font: 12.0px Times}  Feline coronavirus (FCoV) is a highly contagious virus that is ubiquitous in multicat environments and may induce oxidative stress. This virus commonly causes an asymptomatic infection, which can persist in certain individuals. Sporadically and unpredictably, FCoV infection leads to feline infectious peritonitis (FIP), a highly fatal systemic immune-mediated disease. There are no data in the veterinary literature relating to oxidative stress in FCoV. Antioxidant capacity (TAC) can be attributed to single components in the defence systems against free radicals. The measurement of the total oxidant status (TOS) accurately reflects the oxidative status of blood plasma or serum. Nitric oxide (NO) acts as a free radical and contributes to host defences against oxidation. Malondialdehyde (MDA) is a reliable and commonly used marker of overall lipid peroxidation levels and the presence of oxidative stress. This study aimed to determine levels of oxidative stress markers, serum TAC, total oxidant capacity (TOC), NO and serum MDA in 24 cats seropositive for FCoVs and 15 cats seronegative for FCoVs. Significantly higher serum TOC, NO and MDA levels were found in seropositive animals (P < 0.001, P < 0.05 and P < 0.001, respectively) than in seronegative animals. In contrast, serum TAC levels were found to be significantly lower in seropositive cats compared with seronegative cats (P < 0.001). The results of the present study suggest that FCoV seropositivity is associated with oxidative stress and decreased antioxidant status. 

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May 2015

6 Citations

Impact Factor 0.450

4 Reads

A molecular and parasitological survey of Hepatozoon canis in domestic dogs in Turkey.

Vet Parasitol 2015 Apr 25;209(3-4):264-7. Epub 2015 Feb 25.

Department of Parasitology, College of Veterinary Medicine, Firat University, 23119, Elazig, Turkey.

In this study, asymptomatic dogs in nine provinces of Turkey were surveyed to investigate the prevalence and intensity of Hepatozoon canis infection. DNA obtained from blood samples collected from 694 domestic dogs (243 stray, 288 shelter, and 163 pets) of both genders and varying ages were evaluated by polymerase chain reaction (PCR). In addition, 285 thin blood smears prepared from these blood samples were also evaluated for microscopic examination. Direct microscopy revealed Hepatozoon gamonts in the peripheral blood of three of 285 (1.0%; 95% confidence interval (CI): 0.21-3.04) tested. Using PCR, 155 of the 694 (22.3%; 95% CI: 19.28-25.61) were found to be positive for the presence of H. canis DNA. The prevalence of infection was higher in adult dogs (26.2%; 95% CI: 22.1-30.7) than young animals (16.4%; 95% CI: 12.2-21.3). Although the prevalence determined by PCR was higher in male dogs (24.5%; 95% CI: 19.6-29.9) than in female dogs (20.8%; 95% CI: 16.9-25.1), gender differences were not significant. Pet dogs had a lower prevalence of infection (10.4%; 95% CI: 6.2-16.2) compared to stray (26.3%; 95% CI: 20.9-32.3) and shelter dogs (25.7%; 95% CI: 20.7-31.1), but no significant association between stray and shelter dogs was found for the presence of the parasite. Partial sequences of the 18S ribosomal RNA (rRNA) gene shared 99-100% similarity with the corresponding H. canis isolates. This epidemiological survey revealed a high prevalence of H. canis in dogs from several provinces in Turkey, and it suggests that the age and origin are associated with the parasite.

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http://dx.doi.org/10.1016/j.vetpar.2015.02.015DOI Listing
April 2015
168 Reads
20 Citations
2.460 Impact Factor

Rutin attenuates gentamicin-induced renal damage by reducing oxidative stress, inflammation, apoptosis, and autophagy in rats.

Ren Fail 2015 Apr 23;37(3):518-25. Epub 2015 Jan 23.

Department of Biochemistry, Faculty of Veterinary Medicine, Ataturk University , Yakutiye, Erzurum , Turkey .

Gentamicin is commonly used against gram-negative microorganisms. Its therapeutic use is mainly limited by nephrotoxicity. This study was aimed at evaluating the effect of rutin on oxidative stress, inflammation, apoptosis, and autophagy in gentamicin-induced nephrotoxicity in rats. The rats were treated with saline intraperitoneally (group I), 150?mg/kg of rutin orally (group II), 80?mg/kg of gentamicin intraperitoneally for 8?d (group III), or 150?mg/kg of rutin plus 80?mg/kg of gentamicin (group IV). The serum urea, creatinine, kidney malondialdehyde (MDA), and reduced glutathione (GSH) levels and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activity and protein concentration were measured, and renal histopathology analysis and immunohistochemical staining were performed. Rutin pretreatment attenuated nephrotoxicity induced by gentamicin by reducing the urea, creatinine, and MDA levels and increasing the SOD, CAT, and GPx activity, and the GSH levels. The rutin also inhibited inducible nitric oxide synthase (iNOS), cleaved caspase-3 and light chain 3B (LC3B), as evidenced by immunohistochemical staining. The present study demonstrates that rutin exhibits antioxidant, anti-inflammatory, anti-apoptotic, and anti-autophagic effects and that it attenuates gentamicin-induced nephrotoxicity in rats.

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http://dx.doi.org/10.3109/0886022X.2015.1006100DOI Listing
April 2015
32 Reads
20 Citations
0.775 Impact Factor

Molecular detection of tick-borne rickettsial and protozoan pathogens in domestic dogs from Turkey.

Parasit Vectors 2015 Mar 14;8:157. Epub 2015 Mar 14.

Department of Parasitology, College of Veterinary Medicine, Firat University, 23119, Elazig, Turkey.

Background: Canine tick-borne parasites have emerged in recent years, showing a wider geographic distribution and increased global prevalence. In addition to their veterinary importance, domestic dogs play an important role in the transmission cycles of some agents by acting as reservoirs and sentinels. This study investigated Babesia, Theileria, Anaplasma, and Ehrlichia species in asymptomatic dogs in ten provinces of Turkey.

Methods: DNA obtained from blood samples collected from 757 domestic dogs (243 stray, 351 shelter, 163 pet) of both sexes and various ages were evaluated using PCR and reverse line blotting (RLB) assays.

Results: Of the 757 dogs tested, 41 (5.4%) were found to be infected with one or more parasites. Ehrlichia canis (37/757, 4.9%) was the most common canine tick-borne pathogen, followed by Anaplasma platys (4/757, 0.5%). Babesia canis and Theileria annulata were each detected in 1 (0.13%) sample. Combined infection of E. canis and A. platys was detected in 2 (0.3%) samples. The prevalence of tick-borne pathogens was higher in adult dogs (6.8%) than in those under one year old (3.1%). Difference in infection rate of male and female dogs was not significant. Pet dogs had a lower prevalence of infection (1.2%) compared to stray (7.4%) and shelter dogs (6%) although the difference between stray and shelter dogs was not significant.

Conclusions: Babesia canis, T. annulata, A. platys, and E. canis species were identified at the molecular level in dogs in several provinces of Turkey, with E. canis being the most common species among tick-borne pathogens. Detailed studies should be conducted regarding the existence and prevalence of B. canis and Dermacentor reticulatus in eastern Turkey.

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http://dx.doi.org/10.1186/s13071-015-0763-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4364499PMC
March 2015
20 Reads
30 Citations
3.430 Impact Factor

Evaluation of serum cardiac troponin I concentration in sheep with acute ruminal lactic acidosis

4

VETERINARSKI ARHIV

Cardiac troponin I (cTnI) is known to be a sensitive cardio biomarker to determine the myocardial

damage in diseases affecting the cardiac muscles. However, there has not been suffi cient research about cTnI

concentration, which is the most sensitive indicator of myocardial damage in sheep with acute ruminal lactic

acidosis (ARLA). For this reason this study aimed to evaluate the serum cTnI concentration in sheep with

ARLA. Those diagnosed with ARLA (n = 20) from the total of 40 Akkaraman (White karaman) sheep, aged

between 1-2 years used in this study comprised the affected group and the healthy ones (n = 20) comprised

the control group. Ruminal fl uid was obtained from the animals from both groups with the help of a stomach

tube, and examined immediately. Blood samples were taken from the jugular vein of the sheep and the serum

was separated. Serum cTnI concentration was measured with a commercial immunoassay system, using the

one-step sandwich method. Serum enzyme (ALT, AST, CK-MB and LDH) activities were determined via a

clinical biochemistry autoanalyzer. The average serum cTnI concentration was at the level of 0.035 ± 0.015 ng/

mL (range; 0.02-0.06 ng/mL) in the control group sheep. It was determined that there was a substantial increase

in the group with ARLA and the average concentration reached the level of 0.103 ± 0.080 ng/mL (range; 0.03-

1.7 ng/mL) (P<0.0001). It was observed that another cardio marker, CK-MB, was found in the group with

ARLA 454.50 ± 191.88 U/L (range; 214-861 U/L) and increased in comparison with the control group 224.35

± 83.33 U/L (range; 133-421 U/L) (P<0.0001). An increase in LDH (P<0.001) and AST (P<0.001) from liver

enzymes in the group with ARLA and ALT activities compared to the control group was identifi ed (P<0.01). In

conclusion, this present study determined that the serum cTnI concentration was high in sheep with ARLA and

it was concluded that it could be useful to evaluate cTnI concentration as an important marker to determine the

prognosis in sheep with ARLA

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September 2014

4 Citations

Impact Factor 0.320

2 Reads

Status of lipid peroxidation and some antioxidants in sheep with acute ruminal lactic acidosis

2

Med. Weter.

The purpose of this study was to evaluate the status of lipid peroxidation (LPO) and some antioxidants in

sheep with acute ruminal lactic acidosis (ARLA). The material consisted of 20 Akkaraman sheep which had

been diagnosed with ARLA and 20 healthy sheep of the same breed as the control group. The sheep were

1-2 years old. Ruminal fluid was obtained from the animals from both groups by means of a stomach tube

and examined immediately. Blood samples were obtained from the jugular vein of the animals. The activites

of catalase (CAT) and superoxide dismutase (SOD) as well as the levels of reduced glutatione (GSH) and

malondialdehyde (MDA, a marker of LPO) were determined. Compared with the control group, the sheep

with ARLA had a significantly higher level of MDA (p < 0.0001), whereas the activities of CAT and SOD and

the level of GSH were significantly lower (p < 0.0001). In conclusion, this study highlights the occurrence of

oxidative stress with important differences in antioxidant status, as reflected by the assessment of selected

enzymatic and nonenzymatic antioxidants in sheep with ARLA and healthy controls. This indicates that the

serum activities of SOD and CAT, as well as the levels of GSH and MDA, may be useful as indicators of LPO

and antioxidant status in sheep with ARLA.

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June 2014

2 Citations

Impact Factor 0.197

4 Reads

Effects of ramipril and darbepoetin on electromechanical activity of the heart in doxorubicin-induced cardiotoxicity.

Int J Cardiol 2014 May 15;173(3):519-21. Epub 2014 Mar 15.

Department of Cardiology, Faculty of Medicine, Ataturk University, Erzurum, Turkey.

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http://dx.doi.org/10.1016/j.ijcard.2014.03.044DOI Listing
May 2014
9 Reads
12 Citations
4.036 Impact Factor

Sheep-Associated Malignant Catarrhal Fever: First report in a Calf in Northeastern Turkey

Israel Journal of Veterinary Medicine

In this report, systemic vasculitis was described in a Brown Swiss calf with sheep-associated malignant

catarrhal fever. The calf was referred to the university clinic due to respiratory and nervous symptoms.

Nasal discharge, dyspnea, cough, conjunctival hyperemia, bilateral corneal opacity and ulceration, superficial

lymph node enlargement, incoordination and muscle tremors were detected on clinical examination. The

hematologic profile revealed lymphocytosis and neutrophilia. Grossly, hyperemia of viscera, lymphoid tissue

enlargements and swelling in the brain were observed. Fibrinoid necrotic vasculitis and lymphoid cell

infiltrations were main histopathologic changes in the brain, liver, spinal cord, heart, lymphoid tissues and

upper respiratory tract. Characteristic histopathologic findings were confirmed by PCR, which demonstrated

the presence of ovine herpesvirus-2 (OvHV-2) in the lymph nodes and liver samples of the calf.

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September 2013

Impact Factor 0.300

3 Reads

Direct Role of α2-Adrenoreceptors in Antiulcer Effect Mechanism of Tianeptine in Rats

Kafkas Univ Vet Fak Derg

p.p1 {margin: 0.0px 0.0px 0.0px 0.0px; font: 12.0px 'Myriad Pro'; min-height: 14.0px} p.p2 {margin: 0.0px 0.0px 5.0px 0.0px; text-align: justify; text-indent: 14.0px; line-height: 9.1px; font: 9.0px 'Myriad Pro'} span.s1 {font: 12.0px 'Myriad Pro'} span.s2 {font: 5.0px 'Myriad Pro'}  Tianeptine is an anti-depressant drug that also has an anti-ulcer activity. In this study, it was investigated whether or not α2-adrenoreceptors have a role in the anti-ulcer effect mechanism of tianeptine. Furthermore, we investigated both intact and adrenalectomized rats to determine whether or not the anti-ulcer activity of tianeptine is related to adrenal gland hormones involved in this mechanism. In all rats, 25 mg/kg indomethacin produced gastric ulceration. Tianeptine was administered to the indomethacin-induced ulcers in the rats at different doses. Yohimbine, selective α2-receptor blocker, was given (10 mg/kg) to some rats (adrenalectomized 6 groups and intact 4 groups), for blockage of the α2-receptor. Tianeptine showed antiulcer effects of 59.6-81.6% in all animals. The results of this study show that tianeptine caused a significant anti-ulcer activity in both adrenalectomized and intact rats. Tianeptine does not have an anti-ulcer effect in rats which have been given yohimbine. Thus, the adrenal gland hormones do not have a direct effect on the anti-ulcer activity of tianeptine. This drug may be directly related to the α2-adrenoreceptors. Moreover, the anti-ulcer effect shows an increase in parallel with increasing dose. This property of tianeptine could make it suitable for use in the treatment of both depression and peptic ulcer patients.

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September 2012

Impact Factor 0.450

3 Reads

Detection of Toxoplasma gondii Antibodies and Some Helminthic Parasites in Camels from Nevsehir Province of Turkey

6

Israel Journal of Veterinary Medicine

This study was conducted on 11 camels, ranging in age from 2 months to 40 years. The aim of this study

was to detect the Toxoplasma gondii antibodies and helminth parasites in camels. T. gondii antibodies were

detected by Sabin-Feldman Dye Test (SFDT), and faecal samples were examined by Fulleborn flotation and

Benedek sedimentation methods. As a result of the study, T. gondii antibodies were detected in 10 out of

11 (90.9%) camels. Detected helminths consisted of 63.63% (7/11) Trishostrongyloidea spp., 45.45% (5/11)

Trichuris spp. and 9.09% (1/11) Dicrocoelium dendriticum.

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June 2012

6 Citations

Impact Factor 0.300

3 Reads