Publications by authors named "Akane Sato"

14 Publications

  • Page 1 of 1

The vacuolar amino acid transport system is a novel, direct target of GATA transcription factors.

Biosci Biotechnol Biochem 2021 Feb;85(3):587-599

Laboratory of Molecular Physiology and Genetics, Faculty of Agriculture, Ehime University, Matsuyama, Ehime, Japan.

In Saccharomyces cerevisiae, Avt4 exports neutral and basic amino acids from vacuoles. Previous studies have suggested that the GATA transcription factors, Gln3 and Gat1, which are key regulators that adapt cells in response to changes in amino acid status, are involved in the AVT4 transcription. Here, we show that mutations in the putative GATA-binding sites of the AVT4 promoter reduced AVT4 expression. Consistently, a chromatin immunoprecipitation (ChIP) assay revealed that Gat1-Myc13 binds to the AVT4 promoter. Previous microarray results were confirmed that gln3∆gat1∆ cells showed a decrease in expression of AVT1 and AVT7, which also encode vacuolar amino acid transporters. Additionally, ChIP analysis revealed that the AVT6 encoding vacuolar acidic amino acid exporter represents a new direct target of the GATA transcription factor. The broad effect of the GATA transcription factors on the expression of AVT transporters suggests that vacuolar amino acid transport is integrated into cellular amino acid homeostasis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/bbb/zbaa041DOI Listing
February 2021

Expression levels of , , and in cumulus cells can predict developmental competence of bovine oocytes.

Zygote 2020 Jun 2:1-7. Epub 2020 Jun 2.

Department of Animal Production Science, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Tokyo183-8509, Japan.

The efficiency of in vitro embryo production technologies would be improved by the development of suitable non-invasive biomarkers that allow the selection of good quality cumulus-oocyte complexes (COCs). The present study used whole, single oocyte culture to investigate whether the expression levels of follicle-stimulating hormone receptor (FSHR), insulin-like factor 1 receptor (IGF1R) and three steroidogenesis-related enzymes (CYP11al, CYP19al and HSD3β) in cumulus cells reflected the developmental competence of COCs. Cumulus cells were collected from single COCs before maturation culture and relative mRNA levels were assessed using real-time PCR. The analysis indicated that mRNAs for FSHR, IGF1R, CYP11al and HSD3β were present at higher levels in cumulus cells from COCs that failed to form blastocysts compared with cumulus cells from COCs that formed blastocysts. Moreover, FSHR and IGF1R mRNA levels were positively correlated with those of genes for steroidogenesis-related enzymes. In conclusion, poor developmental competence of COCs was related to higher expression of FSHR, IGF1R, CYP11al and HSD3β in cumulus cells, which may indicate the advanced differentiation of cumulus cells into granulosa cells.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1017/S0967199420000283DOI Listing
June 2020

Transrectal ultrasonography and blood lactate measurement: a combined diagnostic approach for severe uterine torsion in dairy cattle.

J Vet Med Sci 2019 Oct 1;81(9):1385-1388. Epub 2019 Aug 1.

Hokkaido Higashi Agricultural Mutual Aid Association, 109-28 Nishishunbetsu, Betsukai-cho, Notsuke-gun, Hokkaido 088-2576, Japan.

In this study, we aimed to elucidate the utility of transrectal ultrasonography (TRUS) and blood lactate concentration (bLac) measurement to diagnose cows with severe uterine torsion. We investigated the association of TRUS and bLac measurement with macroscopic findings on laparotomy for severe uterine torsion in nine cows. We found that an increased ultrasonographic cross-sectional thickness (15-25 mm) and multiple hypoechogenic areas corresponded to macroscopic vascular compromise in the uterus on laparotomy. In addition, bLac was elevated (≥5.0 mmol/l) in cows showing uterine necrosis on laparotomy. A combined diagnostic approach with TRUS and bLac measurement enables assessment of the uterine vascular status and has utility for selecting the treatment option (including laparotomy) and predicting the outcomes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1292/jvms.18-0500DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6785612PMC
October 2019

Duodenal infusion of fatty acids differentially affects plasma glucagon-like peptide-1 and ghrelin concentrations in sheep.

J Anim Sci 2018 May;96(5):1889-1893

University Farm, Department of Agriculture, Utsunomiya University, Mohka, Japan.

The aim of this study was to investigate how intraduodenal infusions of fatty acids (FA) affect appetite-related gut peptides such as glucagon-like peptide-1 (GLP-1) and ghrelin in sheep. We hypothesized that these peptides can be highly reactive to unsaturated long-chain FA, because they are well known to decrease dry matter intake (DMI). Four ewes were fitted with a duodenal cannula and a jugular vein catheter for a 6-h duodenal infusion of the 9 FA (C8:0, C10:0, C12:0, C14:0, C16:0, C18:0, C18:1, C18:2, and C18:3) and water (control). The concentration of each FA was 1.6 g per metabolic body weight (BW), approximately corresponding to the amount of supplemented fat in a standard dairy cow diet. Each infusion was separated by at least 2 d. During the infusion period, blood samples were collected periodically to determine changes in plasma GLP-1, ghrelin, and metabolite concentrations. Duodenal infusions of C18:1, C18:2, and C18:3 led to higher plasma GLP-1 (P < 0.05) and lower glucose (P < 0.05) than control. Plasma ghrelin concentrations were greater in C18:1 and C18:3 infusions than control (P < 0.05). Plasma ketone bodies were higher in C8:0 and C10:0 infusions (P < 0.05), but plasma triglyceride concentrations were lower in C8:0, C10:0, C12:0, and C16:0 infusions (P < 0.05) than control. Fatty acid infusions except for C18:3 led to higher plasma NEFA concentrations than control (P < 0.05). These results confirmed that the hypophagic effect of dietary unsaturated long-chain FA is mediated by GLP-1 (an anorexigenic effect) secretion. However, we also observed higher plasma ghrelin (an orexigenic effect) partially by unsaturated long-chain FA. Thus, the gut peptide secretions when ruminant animals ingest FA supplements would complexly affect satiety and further studies are needed to determine their each impact on DMI.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/jas/sky083DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6140842PMC
May 2018

Histochemical properties of bovine and ovine mammary glands during fetal development.

J Vet Med Sci 2018 Feb 15;80(2):263-271. Epub 2017 Dec 15.

Department of Animal Production Science, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan.

In order to obtain more information on the development of bovine and ovine fetal mammary glands, a series of mammary glands from fetuses of different ages were analyzed. A total of 16 bovine fetuses with curved crown rump lengths ranging from 12 cm (80 days) to 75 cm (240 days) and 15 ovine fetuses ranging from 55 days to 131 days were examined. We used hematoxylin and eosin stain and Oil-Red-O stain to analyze the developmental and morphogenetic processes of mammary glands. In addition, we used immunohistochemical staining to determine the pattern of expression of cytokeratin 18 (CK18) during luminal epithelial differentiation, α-smooth-muscle actin (α-SMA) for myoepithelial differentiation, Ki-67 for cell proliferation, and estrogen receptor α (ERα). Our analyzes showed: (a) The primary mammary duct begin to proliferate in a lengthwise within the teat at 90 days in bovine fetuses and 63 days in ovine fetus; (b) luminal epithelial cells and myoepithelial cells appeared from 90 days in bovine fetuses and 63 days in ovine fetus; (c) proliferation of epithelial cells appeared to coincide with the development of the primary and secondary ducts; and (d) ERα was not found in the fetal mammary gland, but adipocytes showed the presence of ERα. Overall, these results indicate that the sequence of events in the prenatal development of the mammary gland of sheep is similar to that of cattle.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1292/jvms.17-0584DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5836762PMC
February 2018

Effects of insulin-like growth factor-1 on the in vitro maturation of canine oocytes.

J Reprod Dev 2018 Feb 7;64(1):83-88. Epub 2017 Dec 7.

University Farm, Faculty of Agriculture, Utsunomiya University, Tochigi 321-4415, Japan.

The maturation rate of canine oocytes during in vitro maturation (IVM) needs to be improved. The present study was designed to evaluate the effects of insulin-like growth factor-1 (IGF-1) on the IVM of canine oocytes. Ovaries were obtained by ovariohysterectomy and were sliced to release cumulus-oocyte complexes (COCs). In Experiment 1, the effects of different concentrations of IGF-1 on the nuclear maturation of oocytes was investigated. The COCs were cultured in a modified medium (mTCM199) with IGF-1 (0, 0.5, 5, 10, and 50 µg/ml). At the end of the 48 h culture, oocytes were fixed and stained to evaluate their nuclear stage. Supplementation with 50 µg/ml IGF-1 induced a significantly higher metaphase II (MII) rate (P < 0.05) compared to the 0 and 0.5 μg/ml IGF-1 groups. In Experiment 2, the expression levels of insulin receptor (INSR), IGF-1 receptor (IGF-1R), and IGF-2 receptor (IGF-2R) genes, localized to canine oocytes and cumulus cells, were investigated before and after IVM. The expression level of IGF-1R in cumulus cells after IVM was higher than that before IVM (P < 0.05). In Experiment 3, it was investigated whether an inhibitor of PTEN (phosphatase and tensin homolog), bpV, affects the nuclear maturation of oocytes. Regardless of bpV supplementation at a concentration of 0.2 to 200 µmol/l, there was no significant difference in the proportion of oocytes that reached the MII stage. These results indicated that IGF-1 has a favorable effect on the IVM of canine oocytes, possibly through the stimulation of the Ras/MAPK pathway via IGF-1R expressed in cumulus cells.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1262/jrd.2017-145DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5830362PMC
February 2018

Mutations in the vesicular trafficking protein annexin A11 are associated with amyotrophic lateral sclerosis.

Sci Transl Med 2017 05;9(388)

Department of Genome Analysis, University of Amsterdam, Academic Medical Centre, P.O. Box 22700, 1100DE Amsterdam, Netherlands.

Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder. We screened 751 familial ALS patient whole-exome sequences and identified six mutations including p.D40G in the gene in 13 individuals. The p.D40G mutation was absent from 70,000 control whole-exome sequences. This mutation segregated with disease in two kindreds and was present in another two unrelated cases ( = 0.0102), and all mutation carriers shared a common founder haplotype. Annexin A11-positive protein aggregates were abundant in spinal cord motor neurons and hippocampal neuronal axons in an ALS patient carrying the p.D40G mutation. Transfected human embryonic kidney cells expressing with the p.D40G mutation and other N-terminal mutations showed altered binding to calcyclin, and the p.R235Q mutant protein formed insoluble aggregates. We conclude that mutations in are associated with ALS and implicate defective intracellular protein trafficking in disease pathogenesis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1126/scitranslmed.aad9157DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6599403PMC
May 2017

Blood lactate concentration as diagnostic predictors of uterine necrosis and its outcome in dairy cows with uterine torsion.

J Vet Med Sci 2017 Mar 3;79(3):513-516. Epub 2017 Feb 3.

Hokkaido Higashi Agricultural Mutual Aid Association, 109-28 Nishishunbetsu, Betsukai-cho, Notsuke-gun, Hokkaido 088-2576, Japan.

In order to determine blood lactate concentrations (bLac) and their validity as a diagnostic marker in bovine uterine torsion, blood samples were taken from 54 Holstein cows with uterine torsion before the correction of torsion. bLac in a group of cows with and without uterine necrosis were 15.0 and 3.0 mmol/l, respectively (P<0.01). Moreover, bLac in a group of dead or culled dams and in that of survived dams were 10.2 and 3.1 mmol/l, respectively (P<0.01). Furthermore, the proposed diagnostic cutoffs for bLac based on ROC analysis for detection of uterine necrosis and poor prognosis in dams were set at >5.0 and >6.5 mmol/l, respectively. These findings suggest that in dairy cows with uterine torsion, an increase in bLac is a diagnostic predictor of uterine necrosis as well as poor prognosis in dams.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1292/jvms.16-0203DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5383170PMC
March 2017

[A historical review of the therapeutic use of wood creosote. Part II: Original plant source of crude drug wood creosote].

Yakushigaku Zasshi 2011 ;46(1):13-20

Taiko Pharmaceutical Co., Ltd., 3-34-14 Uchihonmachi, Suita, Osaka 564-0032.

Wood creosote is a medicine that has been listed in the Japanese Pharmacopoeia (JP) since the first edition published in 1886. Medicines containing wood creosote and other natural ingredients have been very popular in Japan and Southeast Asian countries. In Japan, one such medicine, named Seirogan, has been used for more than 100 years. In this paper, we report the results of our examination on the historical aspects of wood creosote. One finding was that creosote, called "kereosote" at that time, was imported to Japan for the first time to Nagasaki by Johann Erdewin Niemann, who was the Director of the Dutch Mercantile House, and prescribed by Johannes Lijdius Catharinus Pompe van Meerdervoort and Anthonius Franciscus Bauduin. From our findings, we concluded that wood creosote was one of the essential medicines for the successful introduction and progression of Western medicine in Japan. Furthermore, we found that Dutch physicians introduced wood creosote to Japanese physicians, including Taizen Sato, Dokai Hayashi, and Jun Matsumoto, and that wood creosote was subsequently popularized by Rintaro (Ogai) Mori during the Russo-Japanese war. In addition, we examined the original plant for wood creosote, and consequently confirmed that the 15th edition of the JP, Supplement Two, clarifying the original plant for wood creosote, matches the pharmaceutical and historical facts. We also provide drug information relating to distinguishing between wood creosote and the creosote bush.
View Article and Find Full Text PDF

Download full-text PDF

Source
January 2012

Cultivation and characterization of Lawsonia intracellularis isolated from rabbit and pig.

J Vet Med Sci 2008 Jul;70(7):731-3

Department of Applied Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido, Japan.

Lawsonia intracellularis is an obligate intracellular pathogenic bacterium that causes proliferative enteropathy in domestic and experimental animals. In this study, we improved the in vitro cultivation method of L. intracellularis to increase the passage efficiency and showed that L. intracellularis isolated from a rabbit and a pig have different antigenic properties. Bacteria should be recovered from infected cells before cell death due to infection to obtain higher bacterial passage efficiency, and measurement of LDH activity in the cell culture medium was useful for determining the timing of bacterial passage. L. intracellularis isolated from the rabbit and pig showed different band patterns in immunoblotting. Our results should be helpful in the development of serological diagnosis and epidemiological investigation methods.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1292/jvms.70.731DOI Listing
July 2008

[A historical review of the therapeutic use of wood creosote based on its botanical origin].

Yakushigaku Zasshi 2007 ;42(2):110-8

Taiko Pharmaceutical Co., Ltd., 3-34-14 Uchihonmachi, Suita, Osaka 564-0032.

After thoroughly studying the chronology of the therapeutic use of wood creosote, we obtained novel findings on its botanical origin. Furthermore, we could demonstrate the importance of differentiating between wood creosote and coal tar creosote, which is clearly stipulated by Japanese Pharmacopoeia.
View Article and Find Full Text PDF

Download full-text PDF

Source
July 2008

Alkyl group as entropy reservoir in an MMX chain complex, Pt2(n-PenCS2)4I.

J Phys Chem B 2005 Feb;109(7):2956-61

Research Center for Molecular Thermodynamics, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan.

Heat capacity of halogen-bridged one-dimensional binuclear metal complex (so-called MMX chain) having four n-pentyl groups, Pt2(n-PenCS2)4I, was measured by adiabatic calorimetry. A first-order phase transition was observed at 207.4 K when measurement was made after cooling from room temperature. The enthalpy and entropy of transition were determined to be 10.19 kJ mol(-1) and 49.1 J K(-1) mol(-1), respectively. A monotropic phase transition was observed at 324 K on heating, and the entropy of transition was essentially null. The sample once heated above 324 K never returned to the initial phase at room temperature and underwent a higher-order phase transition at 173 K and a first-order phase transition at 220.5 K. The enthalpy and entropy of the first-order phase transition were estimated to be 11.6 kJ mol(-1) and 52.4 J K(-1) mol(-1), respectively. The magnitude of the entropy gain at the phase transition from the initial room-temperature phase to the high-temperature phase at 324 K shows that in Pt2(n-PenCS2)4I a large amount of entropy reserved in alkyl chain is transferred to dithiocarboxylato groups upon the phase transition, as in the cases of Pt2(n-PrCS2)4I and Pt2(n-BuCS2)4I.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/jp046187oDOI Listing
February 2005

Magnetic resonance imaging of neuronal ceroid lipofuscinosis in a border collie.

J Vet Med Sci 2004 Nov;66(11):1453-6

Laboratory of Comprehensive Veterinary Clinical Studies, Department of Veterinary Medicine, College of Bioresouce Sciences, Nihon University, Kanagawa, Japan.

A castrated male border collie 23 months of age weighing 19.4 kg was referred to the Animal Medical Center of Nihon University with complaints of visual disturbance and behavioral abnormality, hyperacusis and morbid fear. The MRI examination revealed the slight dilated cerebral sulci and cerebellar fissures and left ventricular enlargement. This is the first report of MRI findings of canine neuronal ceroid lipofuscinosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1292/jvms.66.1453DOI Listing
November 2004
-->