Publications by authors named "Aiping Chen"

112 Publications

The Value of Circulating microRNAs for Diagnosis and Prediction of Preeclampsia: a Meta-analysis and Systematic Review.

Reprod Sci 2021 Nov 24. Epub 2021 Nov 24.

Department of Medical Genetic, The Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao, 266003, China.

Preeclampsia (PE) is one of the main causes of maternal death worldwide, but our understanding of the molecular characteristics of disease progression is limited. In this meta-analysis, we aimed to assess the value of peripheral blood microRNAs (miRNAs) as diagnostic and predictive markers of PE. We screened PubMed, Web of Science, and Embase databases; searched articles about "miRNAs and PE" up to November 30, 2020; and conducted biological information and subgroup analysis. We used QUADAS-2 (quality assessment of diagnostic accuracy studies-2) to evaluate the included articles by two independent reviewers, calculated the combined diagnostic and predictive indicators using the random effects model, explored the sources of potential heterogeneity through subgroup analysis, and evaluated publication bias using Deeks' funnel plot asymmetry test using Stata 14.0 and Review Manager 5.3 software. Forty-three miRNAs from 15 studies, including 2042 healthy controls and 2685 PE patients, had a pooled sensitivity of 0.86 (95% CI: 0.81-0.90), specificity of 0.89 (95% CI: 0.85-0.92), and an AUC of 0.94 (95% CI: 0.91-0.96). Moreover, before 20 weeks of gestation, the combined sensitivity was 0.86 (95% CI: 0.75-0.92), and the specificity was 0.90 (95% CI: 0.83-0.95), which indicated that some of the circulating miRNAs had changed significantly before the clinical symptoms appeared in PE patients. Circulating miRNAs have high diagnostic and predictive accuracy and may be used as non-invasive biomarkers for the diagnosis and prediction of PE. However, a large sample prospective study is still needed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s43032-021-00799-6DOI Listing
November 2021

Characteristics and outcomes analysis of ovarian Sertoli-Leydig cell tumors (SLCTs): analysis of 15 patients.

J Ovarian Res 2021 Nov 4;14(1):150. Epub 2021 Nov 4.

Department of Gynecology and Obstetrics, The Affiliated Hospital of Qingdao University, NO.16 Jiangsu Road, Qingdao, 266000, Shandong, China.

Introduction: Because of limited information of Sertoli-Leydig cell tumors (SLCTs), the objective aimed to describe clinical parameters, management and treatment results of SLCTs.

Material And Methods: We retrospectively reviewed 15 cases with SLCTs, who were treated in the Affiliated Hospital of Qingdao University between 2009 and 2020. Data of clinical parameters and treatment was studied.

Results: The age ranged 25-69 years. Elevated testosterone was observed in 4 patients. FIGO-stage: 14 were at Ia(10 moderately differentiated, 3 poorly differentiated, 5 retiform pattern).1 was at Ic. Patients with retiform pattern were more likely to exhibit endocrine function (p = 0.019, w = 0.61) and tumor diameter was significantly bigger in no endocrine function (p = 0.012, d = 1.52). All patients received surgical treatment. 8 received postoperative chemotherapy. The median follow-up was 66 months (20-112 months). 1 patient relapsed within 36 months and received cytoreductive surgery. She survived without disease after recurrence treatment. Of 5 patients who performed fertility sparing surgeries with the desire of childbirth, 3 had full-term pregnancy and 1 experienced a miscarriage. Another one has not tried to conceive.

Conclusion: The prognosis of SLCTs is good. Our data showed patients with retiform pattern were more likely to exhibit endocrine function. The diameter of tumor was significantly bigger in no endocrine function. Conservative surgery is the preferred option for patients with the desire of fertility at stage Ia. Postoperative chemotherapy is advised to cases with high-risk factors, but the most effective chemotherapy regimen is still uncertain.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13048-021-00909-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8570032PMC
November 2021

Optimizing SnO /Fe O Hetero-Nanocrystals Toward Rapid and Highly Reversible Lithium Storage.

Small 2021 Oct 22:e2103532. Epub 2021 Oct 22.

Key Laboratory for Ultrafine Materials of Ministry of Education, Frontiers Science Center for Materiobiology and Dynamic Chemistry, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai, 200237, China.

Engineering oxygen vacancy and boosting Li O reversibility on oxides-based electrode are of significance but remains a challenge in high-power lithium-ion batteries. Herein, the heterogenous SnO /Fe O nanocrystals are demonstrated with tailorable x and y values enabled by a glucose-assisted spray combustion technique. Density functional theory calculations unveil the SnO /Fe O with a maximum x value has the optimal electronic structure, the metallic Fe generated from Fe O can markedly reduce the free energy to break Li-O bonds for accelerating subsequent delithiation process of Li O. Consequently, the optimized SnO /Fe O exhibits a remarkably enhanced electrochemical reversibility and reaction kinetics. After stabilized by reduced graphene oxide, the hybrid delivers a high reversible specific capacity of 1113 mAh g with superior rate performance (474 mAh g at 20 A g ) and long cycle life (negligible loss after 500 cycles at 5 A g ), the oxygen vacancy and microstructure are well-maintained after cycles. This work provides the possibilities for skillfully regulating oxygen vacancy and meantime enhancing Li O reversibility.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/smll.202103532DOI Listing
October 2021

Magnetic resonance imaging (MRI) and Computed topography (CT) analysis of Schatzker type IV tibial plateau fracture revealed possible mechanisms of injury beyond varus deforming force.

Injury 2021 Oct 1. Epub 2021 Oct 1.

Department of Trauma, Jiangsu Province Hospital, Jiangsu, China; First Affiliating Hospital of Nanjing Medical University, China. Electronic address:

Background: Schatzker type IV tibial plateau fractures (type IV TPFs) are known for complex fracture morphology and high frequency of knee subluxation. Varus deforming force has been believed to be the cause but which fails to explain the lateral tibial plateau comminution and the lateral femoral condyle bone edema observed on injury MRI. The purpose of this study is to further explore the mechanisms of injury of type IV TPFs by synthetically analysing the information obtained from MRI and CT of a cohort of patients.

Methods: Between 2010 and 2019, 49 type IV TPFs were surgically treated in our hospital. The patients with complete preoperative CT and MRI were enrolled. They were classified according to OTA/AO and Luo's updated three-column classification (uTCC) after fracture morphology analysing and measuring. Then the injuries of cruciate/collateral ligaments and bone contusion were studied on MRI. The discrepancy between obvious fracture and occult bone contusion/soft tissue disruption among the groups of uTCC were compared and analysed.

Results: Thirty patients were eligible for this study. Under uTCC system, all the cases were caused by varus force according to the tibial plateau angle and were classified into three groups of uTCC referring the posterior tibial slope angle: 4 were into hyperextension-varus, 21 into the extension-varus and 5 into the flexion-varus group. Fracture morphology analysis found in the extension-varus group, there were two distinct subgroups: OTA/AO 41B1.2 (medial+posteromedial columns disruption) and 41B3.3f (41B1.2 +posterolateral column disruption). Injury MRI revealed 28 of the 30 cases had more than 2 ligamentous injuries. The incidences of anterior and posterior cruciate injury were 96.7% and 43.3% respectively while 70% for medial collateral ligament (MCL). Eighteen out of 30 demonstrated apparent lateral femoral condyle bone contusion sign. Chi-square analysis found in the extension-varus group, the posterolateral column comminution was closely associated with lateral femoral condylar contusion (p<0.05) and MCL injuries (p<0.05). This finding and the absence of medial femoral condylar contusion was unlikely caused by uTCC proposed varus deforming force.

Conclusion: In contrast to varus impaction, some type IV TPFs was probably caused by valgus or rotation force.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.injury.2021.09.041DOI Listing
October 2021

Light-Motivated SnO /TiO Heterojunctions Enabling the Breakthrough in Energy Density for Lithium-Ion Batteries.

Adv Mater 2021 Oct 8:e2103558. Epub 2021 Oct 8.

Key Laboratory for Ultrafine Materials of Ministry of Education, Frontiers Science Center for Materiobiology and Dynamic Chemistry, School of Materials Science and Engineering, East China University of Science & Technology, Shanghai, 200237, China.

Powering lithium-ion batteries (LIBs) by light-irradiation will bring a paradigm shift in energy-storage technologies. Herein, a photoaccelerated rechargeable LIB employing SnO /TiO heterojunction nanoarrays as a multifunctional anode is developed. The electron-hole pairs generated by the Li TiO (x ≥ 0) under light irradiation synergistically enhance the lithiation kinetics and electrochemical reversibility of both SnO and TiO . Specifically, the electrons can quickly pour into the SnO and the generated Sn due to the more positive conduction band potentials (vs TiO ), and mean while the holes also promote the intercalation of Li into TiO by reaching charge balance. A remarkable increase in areal specific capacity is therefore achieved from 1.91 to 3.47 mAh cm at 5 mA cm . More impressively, there is no capacity loss even through 100 cycles, which is the best report for photorechargeable LIBs to date, owing to the strong and stable photoresponse current. This finding exhibits a feasible pathway to break the limitation in the energy density of LIBs by the efficient conversion and storage of solar energy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/adma.202103558DOI Listing
October 2021

Symmetry mismatch controlled ferroelastic domain ordering and the functional properties of manganite films on cubic miscut substrates.

Phys Chem Chem Phys 2021 Aug;23(31):16623-16628

Center for Integrated Nanotechnologies (CINT), Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA.

We have studied the magnetotransport properties and strain release mechanisms in ferroelastic La0.9Sr0.1MnO3 (LSMO) epitaxial thin films on SrTiO3 (STO)(001) substrates with different miscut angles. The substrate miscut angle plays a critical role in releasing shear strain and has a huge impact on the properties of the films. The strain relaxes by monoclinic distortion for films on low miscut substrates and for higher miscut substrates, the strain relaxation causes the formation of periodic twin domains with larger periodicities. We observe that the Curie temperature (TC) decreases systematically, and magnetoresistance (MR) increases with increasing the miscut angle. Such changes in the magnetic and transport properties could be due to the increased density of phase boundaries (PBs) with the increase of miscut angle. This work provides a way to tailor film microstructures and subsequent functional properties of other complex oxide films on miscut substrates with symmetry mismatch.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1cp01957kDOI Listing
August 2021

Evaluation of the effect of low molecular weight heparin in unexplained recurrent pregnancy loss: a meta-analysis of randomized controlled trials.

J Matern Fetal Neonatal Med 2021 Jul 26:1-8. Epub 2021 Jul 26.

Department of Obstetrics and Gynecology, the Affiliated Hospital of Qingdao University, Qingdao, China.

Introduction: The effectiveness of low molecular weight heparin (LMWH) in preventing miscarriage of unexplained recurrent pregnancy loss remains controversial. In order to explore the effect of LMWH therapy in unexplained recurrent pregnancy loss, we conducted this meta-analysis.

Methods: We searched four databases PubMed, Cochrane Library, EMBASE, and Clinical Trials.gov (up to February 2020) for the randomized control trials (RCTs) evaluating the effectiveness of LMWH on the treatment of recurrent miscarriage. We used Stata software to perform a meta-analysis. Moreover, we performed analyses of sensitivity and predefined subgroups based on the definition of recurrent miscarriage (e.g. 2 or more miscarriages or 3 or more miscarriages) to search the source of heterogeneity.

Results: 5 studies met the selection criteria, involving 1452 participants. LMWH reduce the risk of miscarriage of women suffering ≥3 miscarriages (RR = 0.46; 95% CI = 0.35-0.61,  = .00), but the risk of miscarriage of women suffering ≥2 miscarriages was not decreased by LMWH (RR = 0.70; 95% CI = 0.57-0.86,  = .26). No substantial influence was found on Live birth rate (RR = 1.19; 95% CI = 0.99-1.43), Preterm birth (RR = 0.95; 95% CI = 0.65-1.38), Preeclampsia (RR = 0.89, 95% CI = 0.45-1.76), Small for gestational age (RR = 0.89; 95% CI = 0.64-1.51).

Conclusion: LMWH treatment may decrease the miscarriage rate in women suffering a history of 3 or more miscarriages, but not reduce the incidence of miscarriage in women suffering a history of 2 or more miscarriages. We need more RCTs to provide robust and reliable results.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/14767058.2021.1957819DOI Listing
July 2021

Effects of Kinesio Tape on Delayed Onset Muscle Soreness: A Systematic Review and Meta-analysis.

Biomed Res Int 2021 31;2021:6692828. Epub 2021 May 31.

School of Health, Fujian Medical University, Fuzhou, China.

Background: Kinesio tape (KT) may be useful for the treatment of delayed onset muscle soreness (DOMS), but there has been no systematic review assessing their efficacy.

Objectives: We conducted a systematic review and meta-analysis to evaluate the efficacy of KT on DOMS.

Methods: We searched seven databases for randomized controlled trials (RCTs) and crossover randomized trials of KT in DOMS, from the earliest date available to December 31, 2019. The primary outcome was muscle soreness. The secondary outcome was muscle strength and serum creatine kinase (CK) level. The risk of bias was evaluated based on the Cochrane criteria. Data were analyzed using RevMan version 5.3.0 software. values < 0.05 were considered statistically significant. Systematic review registration number is CRD42020157052.

Results: Eight trials (six RCTs and two crossover randomized trials) with 289 participants were included. KT use significantly reduced muscle soreness at 48 h (mean difference (MD): -0.67, 95% confidence interval (CI): -1.10 to 0.24, = 0.002) and 72 h postexercise (MD: -0.81, 95% CI: -1.45 to -0.17, = 0.01) but not at 24 h. KT use improved muscle strength at 72 h postexercise (standardized mean difference: 0.35, 95% CI: 0.02 to 0.69, = 0.04) but not at 24 or 48 h. However, the serum CK level at 24, 48, and 72 h postexercise was not better in the KT group relative to the control group.

Conclusions: Current evidence suggests that KT might help to alleviate DOMS after strenuous exercise to improve muscle strength. Thus, using KT on the skin for more than 48 hours postexercise, but not for 24 h, appears more effective at relieving pain and improving muscle strength.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/6692828DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188598PMC
October 2021

Effect of adjuvant therapy on the prognosis in stage I/II uterine carcinosarcoma: A meta-analysis.

J Obstet Gynaecol Res 2021 Jul 28;47(7):2473-2480. Epub 2021 Apr 28.

Department of Gynecology and Obstetrics, The Affiliated Hospital of Qingdao University, Qingdao, China.

Introduction: To assess the efficacy of adjuvant chemotherapy, radiotherapy, or both following the primary surgery on the progression-free survival and 5-year overall survival in patients with stage I/II uterine carcinosarcoma.

Methods: A preliminary investigation was conducted using PubMed and Embase databases to identify relevant studies published up to March, 2020. Pooled hazard ratios (HRs) and 95% confidence intervals (CIs) were calculated by Revman 5.3 software to analysis outcomes.

Results: Six retrospective cohort studies were involved in the analysis, including 1516 patients in observation group, 956 patients in chemotherapy group, 750 patients in radiotherapy group, and 1082 patients in raidochemotherapy group. The results indicated that chemotherapy alone (HR = 0.59, 95% CI = 0.38-0.91, p < 0.05) and radiochemotherapy (HR = 0.35, 95% CI: 0.24-0.53, p < 0.001) were associated with improved progression-free survival in patients. Similarly, pooled results suggested chemotherapy (HR = 0.49, 95% CI = 0.34-0.71, p < 0.001) and radiochemotherapy (HR = 0.46, 95% CI = 0.29-0.72, p < 0.001) promoted the 5-year overall survival compared with observation. However, radiotherapy alone had no statistical significance in improving progression-free survival (HR = 0.80, 95% CI = 0.49-1.29, p = 0.36) and 5-year overall survival (HR = 0.65, 95% CI = 0.38-1.12, p = 0.12).

Discussion: Chemotherapy and radiochemotherapy appeared to be prognostic beneficial to early-stage uterine carcinosarcoma.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/jog.14800DOI Listing
July 2021

Magnetic Texture in Insulating Single Crystal High Entropy Oxide Spinel Films.

ACS Appl Mater Interfaces 2021 Apr 6;13(15):17971-17977. Epub 2021 Apr 6.

Materials Science and Technology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, United States.

Magnetic insulators are important materials for a range of next-generation memory and spintronic applications. Structural constraints in this class of devices generally require a clean heterointerface that allows effective magnetic coupling between the insulating layer and the conducting layer. However, there are relatively few examples of magnetic insulators that can be synthesized with surface qualities that would allow these smooth interfaces and precisely tuned interfacial magnetic exchange coupling, which might be applicable at room temperature. In this work, we demonstrate an example of how the configurational complexity in the magnetic insulator layer can be used to realize these properties. The entropy-assisted synthesis is used to create single-crystal (MgNiFeCoCu)FeO films on substrates spanning a range of strain states. These films show smooth surfaces, high resistivity, and strong magnetic responses at room temperature. Local and global magnetic measurements further demonstrate how strain can be used to manipulate the magnetic texture and anisotropy. These findings provide insight into how precise magnetic responses can be designed using compositionally complex materials that may find application in next-generation magnetic devices.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.1c01344DOI Listing
April 2021

Design, synthesis, and biological activity evaluation of a series of novel sulfonamide derivatives as BRD4 inhibitors against acute myeloid leukemia.

Bioorg Chem 2021 06 22;111:104849. Epub 2021 Mar 22.

Center of Drug Discovery, State Key Laboratory of Natural Medicines, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, PR China; Jiangsu Key Laboratory of Drug Discovery for Metabolic Disease, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, PR China. Electronic address:

Accumulating researches have contributed much effect to discover novel chemotherapeutic drug for leukemia with expeditious curative effect, of which bromodomain-containing protein 4 (BRD4) inhibitor is considered as a eutherapeutic drug which has presented efficient cell proliferation suppression effect. In this study, we disclosed a series of phenylisoxazole sulfonamide derivatives as potent BRD4 inhibitors. Especially, compound 58 exhibited robust inhibitory potency toward BRD4-BD1 and BRD4-BD2 with IC values of 70 and 140 nM, respectively. In addition, compound 58 significantly suppressed cell proliferation of leukemia cell lines HL-60 and MV4-11 with IC values of 1.21 and 0.15 μM. In-depth study of the biological mechanism of compound 58 exerted its tumor suppression effect via down-regulating the level of oncogene c-myc. Moreover, in vivo pharmacokinetics (PK) study was conducted and the results demonstrated better pharmacokinetics features versus (+)-JQ1. In summary, our study discovers that compound 58 represents as a novel BRD4 inhibitor for further investigation in development of leukemia inhibitor with potentiality.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bioorg.2021.104849DOI Listing
June 2021

Low prognostic nutrition index predicts poorer quality of life in late-stage lung cancer.

Ann Palliat Med 2020 Nov 10;9(6):3976-3984. Epub 2020 Nov 10.

Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Sun Yat-sen University, Institute of Pulmonary Diseases, Sun Yat-sen University, Guangzhou, China.

Background: Quality of life (QOL) is one of the most important endpoints in lung cancer care. Both nutritional and immune status reportedly correlate with QOL, so we investigated whether the prognostic nutritional index (PNI), a reliable marker of nutritional and immune status, can predict QOL in late-stage lung cancer.

Methods: We enrolled 80 lung cancer patients and their clinical data including PNI were obtained. The FACT-L questionnaire in Chinese version 4 was administered to every patient.

Results: Of the 80 lung cancer patients, 16 were stage III and 64 were stage IV. The average PNI value was 44.24±5.53. The average FACT-L score was 99.58±21.84, indicating impaired QOL. The FACT-L score in the stage IV group was significantly lower than that in the stage III group (P=0.001), especially for the four subscales of physical, social/family, emotional, and functioning well-being. In the stage IV group, the FACT-L score in the high PNI group was significantly higher than that in the low PNI group (P=0.042), with especially higher score for the physical well-being subscale. PNI was significantly related to both the FACT-L score (r=0.3265, P=0.0085) and physical well-being subscale (r=0.4746, P<0.0001).

Conclusions: PNI is a simple but valuable biomarker of QOL in stage IV lung cancer patients. A lower PNI may indicate the need for detailed QOL evaluation and intervention.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/apm-20-1892DOI Listing
November 2020

Epitaxial Stabilization of Single-Crystal Multiferroic YCrO Thin Films.

Nanomaterials (Basel) 2020 Oct 21;10(10). Epub 2020 Oct 21.

Center for Integrated Nanotechnologies (CINT), Los Alamos National Laboratory, Los Alamos, NM 87545, USA.

We report on the growth of stoichiometric, single-crystal YCrO epitaxial thin films on (001) SrTiO substrates using pulsed laser deposition. X-ray diffraction and atomic force microscopy reveal that the films grew in a layer-by-layer fashion with excellent crystallinity and atomically smooth surfaces. Magnetization measurements demonstrate that the material is ferromagnetic below 144 K. The temperature dependence of dielectric permittivity shows a characteristic relaxor-ferroelectric behavior at = 375-408 K. A dielectric anomaly at the magnetic transition temperature indicates a close correlation between magnetic and electric order parameters in these multiferroic YCrO films. These findings provide guidance to synthesize rare-earth, chromite-based multifunctional heterostructures and build a foundation for future studies on the understanding of magnetoelectric effects in similar material systems.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/nano10102085DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7588968PMC
October 2020

Artificial intelligence based identification of the functional role of hirudin in diabetic erectile dysfunction treatment.

Pharmacol Res 2021 01 11;163:105244. Epub 2020 Oct 11.

School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, 100029, China. Electronic address:

Diabetic erectile dysfunction (DED) hugely affected the patients' sexual life quality. However, there are no satisfactory therapeutic methods and intervention targets for this subtype of erectile dysfunction (ED). Inspired by the clinical practice of traditional Chinese medicine (TCM), we found that hirudin, the main active ingredient in the leech, could ameliorate the ED symptoms of the DED mouse model. To further reveal the underlying mechanism of hirudin, we designed a novel strategy to discover potential targets based on the diagnostic system of TCM, and found that myeloperoxidase (MPO) was a promising target of hirudin. Hirudin directly interacts with MPO and inhibits its activity, thus further decreases the content of oxidized low-density lipoprotein (ox-LDL) in serum. Our results demonstrated that the hirudin could ameliorate the symptoms of DED, and revealed the underlying mechanism of hirudin in regulating the activity of MPO.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.phrs.2020.105244DOI Listing
January 2021

Nipped-B-like Protein Sensitizes Esophageal Squamous Cell Carcinoma Cells to Cisplatin via Upregulation of PUMA.

Technol Cancer Res Treat 2020 Jan-Dec;19:1533033820960726

Experimental Research Center, Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Institute of Cancer and Basic Medicine (IBMC), Chinese Academy of Sciences, Hangzhou, Zhejiang Province, China.

Nipped-B-like protein plays a pivotal role as a cohesin loading factor in the segregation of chromosomes when cells divide. Accumulating evidence indicates that alterations of this protein are involved in human carcinogenesis, especially in the regulation of chemotherapeutic drug response. However, the role of Nipped-B-like protein in esophageal squamous cell carcinoma remains unknown. In this study, we investigated the relevance of Nipped-B-like protein in the regulation of cisplatin sensitivity in esophageal squamous cell carcinoma. Ectopic expression of Nipped-B-like protein inhibited the growth of COLO-680N cells with low endogenous expression levels of Nipped-B-like protein, and increased sensitivity to cisplatin, a commonly used chemotherapy drug for patients with esophageal squamous cell carcinoma. In contrast, loss of Nipped-B-like protein stimulated the growth of EC9706 and Eca-109 cells with high levels of the protein, and resulted in resistance to cisplatin. P53-upregulated modulator of apoptosis, which is essential in the modulation of cisplatin sensitivity in a variety of cancers, acts as a downstream effector of Nipped-B-like protein. Restoration of this pro-apoptotic protein in Nipped-B-like protein-overexpressing esophageal squamous cell carcinoma cells effectively increased cisplatin sensitivity. Conversely, the silencing of P53-upregulated modulator of apoptosis in Nipped-B-like protein-depleted esophageal squamous cell carcinoma rendered cells resistant to cisplatin. Moreover, Nipped-B-like protein could bind directly to the promoter region of P53-upregulated modulator of apoptosis. In summary, our study addresses the involvement of Nipped-B-like protein in the development of esophageal squamous cell carcinoma, and the modulation of cisplatin sensitivity via regulation of P53-upregulated modulator of apoptosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/1533033820960726DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7592177PMC
November 2021

Combined Metabolomic Analysis of Plasma and Tissue Reveals a Prognostic Risk Score System and Metabolic Dysregulation in Esophageal Squamous Cell Carcinoma.

Front Oncol 2020 26;10:1545. Epub 2020 Aug 26.

Institute of Cancer and Basic Medicine (ICBM), Chinese Academy of Sciences, Hangzhou, China.

Esophageal squamous cell carcinoma (ESCC) is a gastrointestinal malignancy with a poor prognosis. Although studies have shown metabolic reprogramming to be linked to ESCC development, no prognostic metabolic biomarkers or potential therapeutic metabolic targets have been identified. The present study investigated some circulating metabolites associated with overall survival in 276 curatively resected ESCC patients using liquid chromatography/mass spectrometry metabolomics and Kaplan-Meier analysis. Tissue metabolomic analysis of 23-paired ESCC tissue samples was performed to discover metabolic dysregulation in ESCC cancerous tissue. A method consisting of support vector machine recursive feature elimination and LIMMA differential expression analysis was utilized to select promising feature genes within transcriptomic data from 179-paired ESCC tissue samples. Joint pathway analysis with genes and metabolites identified relevant metabolic pathways and targets for ESCC. Four metabolites, kynurenine, 1-myristoyl-glycero-3-phosphocholine (LPC(14:0)sn-1), 2-piperidinone, and hippuric acid, were identified as prognostic factors in the preoperative plasma from ESCC patients. A risk score consisting of kynurenine and LPC(14:0)sn-1 significantly improved the prognostic performance of the tumor-node-metastasis staging system and was able to stratify risk for ESCC. Combined tissue metabolomic analysis and support vector machine recursive feature elimination gene selection revealed dysregulated kynurenine pathway as an important metabolic feature of ESCC, including accumulation of tryptophan, formylkynurenine, and kynurenine, as well as up-regulated indoleamine 2,3-dioxygenase 1 in ESCC cancerous tissue. This work identified for the first time four potential prognostic circulating metabolites. In addition, kynurenine pathway metabolism was shown to be up-regulated tryptophan-kynurenine metabolism in ESCC. Results not only provide a metabolite-based risk score system for prognosis, but also improve the understanding of the molecular basis of ESCC onset and progression, and as well as novel potential therapeutic targets for ESCC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2020.01545DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7479226PMC
August 2020

Molecular Mechanisms of Action of Emodin: As an Anti-Cardiovascular Disease Drug.

Front Pharmacol 2020 27;11:559607. Epub 2020 Aug 27.

College of Pharmaceutical Sciences, Pharmaceutical Informatics Institute, Zhejiang University, Hangzhou, China.

Emodin is a natural occurring anthraquinone derivative isolated from roots and barks of numerous plants, molds, and lichens. It is found to be an active ingredient in different Chinese herbs including and , and it is a pleiotropic molecule with diuretic, vasorelaxant, anti-bacterial, anti-viral, anti-ulcerogenic, anti-inflammatory, and anti-cancer effects. Moreover, emodin has also been shown to have a wide activity of anti-cardiovascular diseases. It is mainly involved in multiple molecular targets such as inflammatory, anti-apoptosis, anti-hypertrophy, anti-fibrosis, anti-oxidative damage, abnormal, and excessive proliferation of smooth muscle cells in cardiovascular diseases. As a new type of cardiovascular disease treatment drug, emodin has broad application prospects. However, a large amount of evidences detailing the effect of emodin on many signaling pathways and cellular functions in cardiovascular disease, the overall understanding of its mechanisms of action remains elusive. In addition, by describing the evidence of the effects of emodin in detail, the toxicity and poor oral bioavailability of mice have been continuously discovered. This review aims to describe a timely overview of emodin related to the treatment of cardiovascular disease. The emphasis is to summarize the pharmacological effects of emodin as an anti-cardiovascular drug, as well as the targets and its potential mechanisms. Furthermore, the treatment of emodin compared with conventional cardiovascular drugs or target inhibitors, the toxicity, pharmacokinetics and derivatives of emodin were discussed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fphar.2020.559607DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7481471PMC
August 2020

Induced ferroelectric phases in SrTiO by a nanocomposite approach.

Nanoscale 2020 Sep 28;12(35):18193-18199. Epub 2020 Aug 28.

Center for Integrated Nanotechnologies (CINT), Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA.

Inducing new phases in thick films via vertical lattice strain is one of the critical advantages of vertically aligned nanocomposites (VANs). In SrTiO (STO), the ground state is ferroelastic, and the ferroelectricity in STO is suppressed by the orthorhombic transition. Here, we explore whether vertical lattice strain in three-dimensional VANs can be used to induce new ferroelectric phases in SrTiO:MgO (STO:MgO) VAN thin films. The STO:MgO system incorporates ordered, vertically aligned MgO nanopillars into a STO film matrix. Strong lattice coupling between STO and MgO imposes a large lattice strain in the STO film. We have investigated ferroelectricity in the STO phase, existing up to room temperature, using piezoresponse force microscopy, phase field simulation and second harmonic generation. We also serendipitously discovered the formation of metastable TiO nanocores in MgO nanopillars embedded in the STO film matrix. Our results emphasize the design of new phases via vertical epitaxial strain in VAN thin films.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0nr03460fDOI Listing
September 2020

Apigenin induces apoptosis and counteracts cisplatin-induced chemoresistance via Mcl-1 in ovarian cancer cells.

Exp Ther Med 2020 Aug 11;20(2):1329-1336. Epub 2020 Jun 11.

Department of Gynecology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266000, P.R. China.

Ovarian cancer (OC) is one of the prominent causes of mortality in female patients diagnosed with gynecologic malignancies. While it has previously been demonstrated that apigenin inhibits cell growth in colon and breast cancer cells, the effect of apigenin in OC cells is not fully understood. Therefore, the aim of the present study was to investigate the impact of apigenin on cell death and resistance to cisplatin in OC cells. It was found that apigenin inhibited proliferation, hindered cell cycle progression and promoted SKOV3 cell apoptosis. Moreover, these effects were also observed in cisplatin-resistant SKOV3/DDP cells. Furthermore, apigenin reduced the mitochondrial transmembrane potential, and elevated the ratios of cleaved caspase-3/caspase-3 and Bax/Bcl-2 in the two cell types. Reverse transcription-quantitative PCR and western blotting results demonstrated that apigenin significantly downregulated Mcl-1 at the transcriptional and translational levels in SKOV3 and SKOV3/DDP cells, which was responsible for its cytotoxic functions and chemosensitizing effects. Collectively, the present results identified the impact of apigenin on OC cell death and resistance to cisplatin, and the potential molecular mechanisms. However, additional studies are required to further elucidate the underlying mechanisms.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3892/etm.2020.8880DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7388300PMC
August 2020

Structural and Optical Properties of Phase-Pure UO, α-UO, and α-UO Epitaxial Thin Films Grown by Pulsed Laser Deposition.

ACS Appl Mater Interfaces 2020 Aug 27;12(31):35232-35241. Epub 2020 Jul 27.

Center for Integrated Nanotechnologies (CINT), Los Alamos National Laboratory, Los Alamos, New Mexico 87545, United States.

Fundamental understanding of the electronic, chemical, and structural properties of uranium oxides requires the synthesis of high-crystalline-quality epitaxial films of different polymorphs of one material or different phases with various oxygen valence states. We report the growth of single-phase epitaxial UO, α-UO, and α-UO thin films using pulsed laser deposition. Both oxygen partial pressure and substrate temperature play critical roles in determining the crystal structure of the uranium oxide films. X-ray diffraction and Raman spectroscopy demonstrate that the films are single phase with excellent crystallinity and epitaxially grown on a variety of substrates. Chemical valance states and optical properties of epitaxial uranium oxide films are studied by X-ray photoelectron spectroscopy and UV-vis spectroscopy, which further confirm the high-quality stoichiometric phase-pure uranium oxide thin films. Epitaxial UO films show a direct band gap of 2.61 eV, while epitaxial α-UO and α-UO films exhibit indirect band gaps of 1.89 and 2.26 eV, respectively. The ability to grow high-quality epitaxy actinide oxide thin films and to access their different phases and polymorphous will have significant benefits to the future applications in nuclear science and technology.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.0c08635DOI Listing
August 2020

Separation and Detection of meta- and ortho-Substituted Benzene Isomers by Using a Water-Soluble Pillar[5]arene.

Chempluschem 2020 06;85(6):1244-1248

Smart Hybrid Materials Laboratory (SHMs) Advanced Membranes and Porous Materials Center (AMPMC), King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Saudi Arabia.

Efficient and energy-saving separation of benzene isomers bearing a diverse range of functional groups is a great challenge due to their overlapping physicochemical properties. Here, we report the successfully use of a water-soluble pillar[5]arene (WP5) as a multifunctional material for the separation and detection of meta/ortho-substituted benzene isomers in water. A liquid-liquid extraction strategy was used for the separation of these benzene isomers based on their different affinity for WP5 in water. The selectivities for the meta over the ortho isomer for xylenes, chlorotoluene, and bromotoluene was 88.6 %, 88.3 %, and 95.0 % respectively, in one extraction cycle. Furthermore, a fluorescence indicator system based on WP5 and a fluorescent dye molecule (10-methylacridinium, D) was adopted and exhibited significant fluorescence and optical discrimination upon the addition of meta- compared to ortho-xylene, which implies that a simple "turn-on" detection can be performed prior to engaging in the separation process.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/cplu.202000275DOI Listing
June 2020

Detection of human cytomegalovirus in patients with epithelial ovarian cancer and its impacts on survival.

Infect Agent Cancer 2020 15;15:23. Epub 2020 Apr 15.

1Medical College of Qingdao University, Qingdao, Shandong Province China.

Background: The cause of epithelial ovarian cancer (EOC) is not elucidated. Viral infection may induce chronic inflammatory infection and play a role in the pathogenesis of cancers. Some viruses are considered to be oncomodulatory, modulating cellular pathways such as cell proliferation, tumor progression, vascular disease development, and immune evasion. Human cytomegalovirus (HCMV) has been detected in several types of cancers including ovarian cancer. However, the role of HCMV in ovarian carcinogenesis remains controversial.

Objective: To investigate the potential role of HCMV infection in EOC, we evaluated the prevalence of HCMV proteins in EOC tissue and its impacts on patients' survival.

Methods: Formalin-fixed paraffin-embedded tissues from 66 patients with EOC and 30 patients with benign ovarian cystadenoma were studied. Specimens were analyzed for expression of HCMV immediate early protein (IE) and HCMV tegument protein (pp65) by immunohistochemistry.

Results: HCMV-IE protein expression was detected in 82% of EOC and 36% of benign tumors; pp65 was detected in 97% of EOC and 63% of benign tumors. Extensive HCMV-IE protein expression was associated with higher stage of EOC. Reactivation of latent HCMV within the tumor at interval debulking surgery may be induced by neoadjuvant chemotherapy before surgery. Extensive HCMV-IE expression was associated with shorter median overall survival than focal or negative expression (39 versus 41 months,  = 0.03). Multivariate analysis indicated that HCMV-IE expression was an independent prognostic factor for overall survival ( = 0.034).

Conclusions: This study demonstrate a high prevalence of HCMV proteins in tissue sections from patients with EOC. HCMV infection can be potential risk factor for EOC development. Extensive HCMV-IE expression indicated a poor prognosis. The relationship between HCMV and clinical outcomes highlight the need for further researches on the oncomodulatory role of HCMV in ovarian cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13027-020-00289-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161202PMC
April 2020

Performance of CT Compared with F-FDG PET in Predicting the Efficacy of Nivolumab in Relapsed or Refractory Hodgkin Lymphoma.

Radiology 2020 06 14;295(3):651-661. Epub 2020 Apr 14.

From the Dept of Radiology, New York Presbyterian Hosp, Columbia Univ Medical Ctr, New York, NY (F.Z.M., L.D., A.C., L.H.S.); Dept of Radiology, Rangueil Univ Hosp, Toulouse, France (F.Z.M.); Dept of Radiology, First Affiliated Hosp of Nanjing Medical Univ, Nanjing, China (A.C.); Univ Lille, CHU Lille, EA 7365-GRITA-Groupe de Recherche sur les Formes Injectables et les Technologies Associées, Lille, France (F.M.); Dept of Hematology, Centre Henri Becquerel, Rouen, France (A.S.); Dept of Hematology, Paoli-Calmette Inst, Marseille, France (J.M.S.d.C.); Dept of Nuclear Medicine, Saint-Louis Hosp, AP-HP, Paris, France (L.V.); Dept of Hematology, Univ Hosp of Dijon, Dijon, France (O.C.); Dept of Hematology, Univ Hosp of Besançon, Besançon, France (A.C.); Dept of Hematology, Univ Hosp of Reims, Reims, France (A.D.); Dept of Hematology, Leon Berard Ctr, Lyon, France (E.N.V.); Dept of Hematology, Univ Hosp of Lyon, Lyon, France (H.G.); Dept of Hematology, Univ Hosp of Angers, Angers, France (M.P.M.M.); Dept of Hematology, Bergonie Inst, Bordeaux, France (A.S.); Dept of Hematology, Saint-Antoine Hosp, AP-HP, Paris, France (R.D.); Dept of Hematology, Univ Hosp of Bordeaux, Bordeaux, France (K.B.); Dept of Hematology, Institut Universitaire du Cancer Toulouse-Oncopole, Toulouse, France (C.B.); Dept of Hematology, Univ Hosp of Limoges, Limoges, France (M.T.); Dept of Hematology, Cochin Hosp, AP-HP, Paris, France (B.D.F.); Centre Antoine Lacassagne, Nice, France (F.P.); Dept of Nuclear Medicine, Institut Curie, Paris, France (R.D.S.); Dept of Hematology, Univ Hosp of Rennes, Rennes, France (G.M., R.H.); INSERM, U1236, Rennes, France (G.M., R.H.); and UMR1015, Institut Gustave Roussy, Université Paris Saclay, Villejuif 94800, France (L.D.).

Background CT and fluorine 18 (F) fluorodeoxyglucose (FDG) PET/CT performances following immune therapy are not well known in patients with relapsed or refractory Hodgkin lymphoma (RRHL). Purpose To compare CT and PET/CT for prognostic value of early response evaluation following nivolumab therapy. Materials and Methods This retrospective study included patients from 34 institutions who underwent early imaging response evaluation from July 2013 to April 2017. Three experienced readers classified imaging response by using Cheson et al and 2016 Lymphoma Response to Immunomodulatory Therapy Criteria as follows: complete (metabolic) response, partial (metabolic) response, stable disease or no metabolic response, or progressive (metabolic) disease. Primary CT and PET assessments were performed at a median of 2.0 months (interquartile range, 1.7-3.7 months) after nivolumab initiation. Kaplan-Meier analysis was used to determine the relationship of primary CT and PET assessment response categories to overall survival (OS). Agreements between primary and secondary imaging assessments were assessed by using κ analysis. Results A total of 45 patients (median age, 37 years; range, 18-77 years; 25 men) underwent a primary assessment using CT and PET/CT; 36 patients also underwent a subsequent assessment. Eleven patients (24%) died after a median follow-up of 21.2 months. CT and PET response categories were associated with OS ( = .03 for primary CT assessment; = .02 for primary PET assessment). There was no pseudoprogression at primary CT and PET assessments. At the primary assessment, response categories by using CT were reclassified by using PET in 44% (20 of 45) of patients. Among these, 55% (11 of 20) were reclassified to complete metabolic response (complete metabolic response rate: 29% [13 of 45 patients] vs complete response rate: 4% [two of 45 patients]), with a 2-year OS probability of 100%. At the secondary assessment, complete response rate using CT increased to 17% (six of 36 patients), hence a better agreement with PET (κ = 0.78; < .001). Conclusion Early CT and PET/CT at a median of 2 months after initiation of nivolumab predicted overall survival in relapsed or refractory Hodgkin lymphoma. Early PET detected additional patients with complete metabolic response. © RSNA, 2020 See also the editorial by Scott and Wang in this issue.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1148/radiol.2020192056DOI Listing
June 2020

Smad4 induces cell death in HO-8910 and SKOV3 ovarian carcinoma cell lines via PI3K-mTOR involvement.

Exp Biol Med (Maywood) 2020 05 10;245(9):777-784. Epub 2020 Apr 10.

Department of Gynecology, the Affiliated Hospital of Qingdao University, Qingdao 266000, People's Republic of China.

Impact Statement: This study investigated the effect and mechanism of Smad4 in ovarian carcinoma (OC) cell viability and demonstrated that Smad4 acted as a tumor suppressor in OC, which may contribute to the understanding of molecular mechanisms underlying OC occurrence and progression. Smad4 expression was decreased in the OC specimens, but Smad4 recovery in the OC cell lines impaired the survival and viability of OC cells by increasing autophagy and apoptosis. Further investigation showed that Smad4 interacted with the P85 subunit of PI3K and caused deactivation of the PI3K/mTOR pathway. Therefore, Smad4 could be considered as a target in cancer therapy due to its regulatory effect in OC carcinogenesis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/1535370220916709DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7273890PMC
May 2020

Hypomethylation of DNA promoter upregulates ADAMTS7 and contributes to HTR-8/SVneo and JEG-3 cells abnormalities in pre-eclampsia.

Placenta 2020 04 24;93:26-33. Epub 2020 Feb 24.

Medical Genetic Department, The Affiliated Hospital of Qingdao University, Qingdao, 266003, China; Prenatal Diagnosis Center, The Affiliated Hospital of Qingdao University, Qingdao, 266003, China. Electronic address:

Introduction: Accumulating evidences have suggested a crucial role of epigenetics in the initiation and progression of pre-eclampsia (PE). Here, we studied the expression of the metalloproteinase ADAMTS7 and the methylation level of its promoter in PE placentas and investigated ADAMTS7 role in the pathogenesis of PE.

Methods: We first explored ADAMTS7 expression in PE and normal placentas by reverse transcription quantitative PCR (RT-qPCR), western blot, and immunohistochemistry. Methylation specific PCR (MSP) and bisulfite sequencing PCR (BSP) were performed to evaluate the methylation status of ADAMTS7 promoter. Treatment with 5'-Aza was used to induce demethylation and thereby to explore the direct relationship between promoter methylation and ADAMTS7 expression. CCK8 assay, colony formation assay, and trans-well assay were conducted to assess the viability, migration, and invasion of HTR-8/SVneo and JEG-3 cells.

Results: Our results showed that ADAMTS7 expression was upregulated in PE placentas. Methylation analysis revealed a hypomethylated status of ADAMTS7 promoter regions in PE placenta tissues. Besides, demethylation induced by 5'-Aza directly restored ADAMTS7 expression in trophoblast cells. Finally, overexpression of ADAMTS7 inhibited viability, migration, and invasion of HTR-8/SVneo and JEG-3 cells, while silence of ADAMTS7 by RNA interference reciprocally facilitated cell viability, migration and invasion in vitro.

Discussion: Upregulation of ADAMTS7 by promoter hypomethylation in placenta might contribute to the etiology of PE via suppressing cell functions of trophoblasts.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.placenta.2020.02.013DOI Listing
April 2020

Study of the effect of metformin on expression levels of TNF-α and IL-18 in animal models of polycystic ovary syndrome.

Minerva Med 2021 Oct 20;112(5):658-660. Epub 2020 Mar 20.

Department of Gynecology, the Affiliated Hospital of Qingdao University, Qingdao, China -

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.23736/S0026-4806.20.06477-0DOI Listing
October 2021

Haplotype Analysis of Candidate Genes Involved in Inflammation and Oxidative Stress and the Susceptibility to Preeclampsia.

J Immunol Res 2020 26;2020:4683798. Epub 2020 Feb 26.

Medical Genetic Department, Prenatal Diagnosis Center, The Affiliated Hospital of Qingdao University, Qingdao, China.

Unbalanced inflammatory reactions and oxidative stress are inseparably interconnected, and both may play crucial roles in the pathophysiological mechanisms of preeclampsia (PE). In the published previous studies, we have genotyped for SNPs that related to inflammation (rs2227485, rs153109, rs17855750, rs2027432, rs2275913, rs763780, rs4819554, and rs13015714) and oxidative stress (rs1695, rs4680, rs1800566, rs4807542, rs713041, rs7579, rs230813, rs1004467, rs3824755, and rs9932581) to investigate whether these polymorphisms were associated with susceptibility to PE in a Chinese Han population. In this present study, we collected these data of experimental and clinical from above studies for haplotype analysis of inflammation-related SNPs in 631 PE patients and 720 normal pregnancy and oxidative stress-related SNPs in 342 PE patients and 457 normal pregnancies for susceptibility to PE. The data of genotype distribution and allele frequency comparisons after correction for multiple comparisons (P/8 or P/10) showed 2 among the 8 candidate inflammation-related SNPs have significant differences (rs2027432 genotype = 407.377, < 0.001, < 0.00625). Moreover, the minor alleles of rs2027432 T (minor allele  = 450.923, < 0.001, < 0.00625; OR = 21.439, 95%CI = 15.181-30.278) and rs4819554 G (minor allele  = 163.465, < 0.001, < 0.00625; OR = 5.814, 95%CI = 4.380-7.719) were confirmed as risk allele of PE, respectively. Our analysis revealed rs2027432 (TT) of NLRP3 and rs4819554 (GG) of IL-17RA are risk factors for PE. However, no significant difference was found at the oxidative stress-related SNPs. In the candidate loci for oxidative stress, we also identified 3 SNP matches (rs4807542 and rs713041, rs230813 and rs75799, rs1004467 and rs3824755) that had high linkage disequilibrium (LD) with each other and were selected as a block ( = 0.98, = 0.97, = 0.97, > 0.9), and the GT and GC haplotypes of rs4807542 and rs713041 in showed significant differences between the PE and control groups ( = 5.143, = 0.0233, < 0.05; = 6.373, = 0.0116, < 0.05). So, we inferred that polymorphisms of NLRP3 rs2027432 and IL-17RA rs4819554, which are related to inflammation, and the rs713041 variant of , which is related to oxidative stress, were associated with susceptibility to PE. The GT and GC haplotypes of rs4807542 and rs713041 in may increase the risk of PE in the Chinese Han population.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2020/4683798DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7061132PMC
September 2020

Hypomethylation of CTGF Promoter in Placenta and Peripheral Blood of Pre-eclampsia Women.

Reprod Sci 2020 02 3;27(2):468-476. Epub 2020 Feb 3.

Prenatal Diagnosis Center, The Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao, 266003, China.

Pre-eclampsia (PE) is a serious pregnancy complication defined as hypertension combined with proteinuria. Previous report has indicated that the expression of connective tissue growth factor (CTGF) is upregulated in PE placentas. In this study, we aim to investigate the potential association between the methylation of the CTGF promoter and PE. The methylation status of CTGF promoter was assessed by methylation-specific PCR and the expression of CTGF was analyzed by real-time quantitative PCR and immunohistochemistry staining, in placental samples from PE patients and normal pregnant women. The methylation level of the CTGF promoter was also analyzed in peripheral blood DNA samples from 90 PE patients and 94 normal pregnant women and further confirmed by methylation-sensitive restriction enzyme digestion combined with real-time PCR. We found a decreased methylation rate of CTGF promoter in PE placentas compared with normal control. In addition, the frequency of hemimethylated (UM) promoter of CTGF in peripheral blood was significantly lower in the PE group (P = 0.019), while the frequency of overall methylated (MU + MM) promoter of CTGF in peripheral blood was lower (borderline significance P = 0.043) in the PE group. The percentage of methylated CpG in CTGF promoter was 30% lower in maternal blood of PE subjects than in controls. Moreover, the lower methylation frequency of CTGF promoter was in accordance with the higher expression of CTGF in trophoblast cells. Together, our data suggested an association between PE and the hypomethylation of CTGF promoter both in placentas and peripheral blood from a Chinese Han population.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s43032-019-00038-zDOI Listing
February 2020

C-Myc-activated long non-coding RNA PVT1 enhances the proliferation of cervical cancer cells by sponging miR-486-3p.

J Biochem 2020 Jun;167(6):565-575

Department of Gynecology, The Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Shinan District, Qingdao 266000, Shandong, China.

Cervical cancer is one of the most prevalent gynecological malignancies. Although the functions of long non-coding RNA (lncRNA) plasmacytoma variant translocation 1 (PVT1) and c-Myc in tumorigenesis have been acknowledged, the roles of c-Myc and lncRNA-PVT1 in the proliferation of cervical cancer are still unclear. Our study is designed to demonstrate the regulatory network involving c-Myc and lncRNA-PVT1 in cervical cancer. Quantitative real-time PCR and western blot assays were performed in our research to estimate the expression levels of RNA and proteins. CCK8 assays were applied to demonstrate the viability of HeLa and SiHa cells. Immunofluorescence assay was then used to investigate the co-localization of lncRNA-PVT1 and miR-486-3p. Binding of c-Myc to the promoter region of PVT1 was identified by ChIP-assay. Functionally, upregulation of lncRNA-PVT1 enhanced the proliferation and viability of cervical cancer cells. Mechanistically, lncRNA-PVT1 sponged miR-486-3p and released its repression of extracellular matrix protein 1. Besides, c-Myc functioned as an activator of lncRNA-PVT1 and upregulated its expression by binding to the promoter of PVT1 in cervical cancer cells. lncRNA-PVT1 was upregulated by c-Myc and thus enhanced the proliferation of cervical cancer cells by sponging miR-486-3p.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/jb/mvaa005DOI Listing
June 2020

Interface Engineered Room-Temperature Ferromagnetic Insulating State in Ultrathin Manganite Films.

Adv Sci (Weinh) 2020 Jan 11;7(1):1901606. Epub 2019 Nov 11.

Department of Materials Science and Metallurgy University of Cambridge 27 Charles Babbage Road Cambridge CB3 0FS UK.

Ultrathin epitaxial films of ferromagnetic insulators (FMIs) with Curie temperatures near room temperature are critically needed for use in dissipationless quantum computation and spintronic devices. However, such materials are extremely rare. Here, a room-temperature FMI is achieved in ultrathin LaBaMnO films grown on SrTiO substrates via an interface proximity effect. Detailed scanning transmission electron microscopy images clearly demonstrate that MnO octahedral rotations in LaBaMnO close to the interface are strongly suppressed. As determined from in situ X-ray photoemission spectroscopy, O -edge X-ray absorption spectroscopy, and density functional theory, the realization of the FMI state arises from a reduction of Mn e bandwidth caused by the quenched MnO octahedral rotations. The emerging FMI state in LaBaMnO together with necessary coherent interface achieved with the perovskite substrate gives very high potential for future high performance electronic devices.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/advs.201901606DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947487PMC
January 2020
-->