Publications by authors named "Aiping Cao"

16 Publications

  • Page 1 of 1

Intervention study of Snyder's hope theory on the stigma of stroke in young and middle-aged patients: a randomised trial.

Ann Palliat Med 2021 May 7;10(5):5721-5728. Epub 2021 May 7.

Department of Rehabilitation Medicine, Affiliated Haian Hospital of Nantong University, Nantong, China.

Background: Stigma refers to the sense of discrimination that society has towards a certain group of people, and this part of the group will also have a sense of shame due to their own negative circumstance. Young and middle-aged stroke patients need long-term effective support from their families and society, which can easily produce shame, and have a negative impact on disease treatment.

Methods: A total of 94 young and middle-aged stroke patients admitted to our hospital from November 2018 to November 2020 were selected and randomly allocated to 2 groups, with 47 cases in each group. The control group received routine intervention, and the observation group received SHT intervention. The stigma, hope level, compliance with functional exercise, National Institute of Health Stroke Scale (NIHSS) score and changes in activities of daily living were compared between the 2 groups before and after intervention.

Results: The total scores of social interaction, treatment, ability, and stigma of the observation group were lower than those of the control group after 1 month of intervention (P<0.05). The observation group's positive attitude towards reality and the future, taking positive actions, maintaining close relationships with others, and hope levels after 1 month of intervention were all higher than the control group (P<0.05). The functional exercise compliance of the observation group was higher than that of the control group after 1 month of intervention (P<0.05). The Barthel index score of the observation group was higher than that of the control group after 1 month of intervention, and the NIHSS score was lower than that of the control group (P<0.05).

Conclusions: The application of SHT to young and middle-aged stroke patients can reduce stigma, improve hope level and compliance with functional exercise, improve neurological function, and enhance the ability for daily living.

Trial Registration: Chinese Clinical Trial Registry ChiCTR2100045926.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/apm-21-441DOI Listing
May 2021

Evolutionary and functional analyses demonstrate conserved ferroptosis protection by Arabidopsis GPXs in mammalian cells.

FASEB J 2021 Jun;35(6):e21550

Ministry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences, Hainan Normal University, Haikou, China.

Species have evolved unique mechanisms to combat the effects of oxidative stress inside cells. A particularly devastating consequence of an unhindered oxidation of membrane lipids in the presence of iron results in cell death, known as ferroptosis. Hallmarks of ferroptosis, including peroxidation of polyunsaturated fatty acids, are conserved among animals and plants, however, early divergence of an ancestral mammalian GPX4 (mGPX4) has complicated our understanding of mechanistic similarities between species. To this end, we performed a comprehensive phylogenetic analysis and identified that orthologous Arabidopsis GPXs (AtGPXs) are more highly related to mGPX4 than mGPX4 is to other mammalian GPXs. This high degree of conservation suggested that experimental substitution may be possible. We, therefore, ectopically expressed AtGPX1-8 in ferroptosis-sensitive mouse fibroblasts. This substitution experiment revealed highest protection against ferroptosis induction by AtGPX5, as well as moderate protection by AtGPX2, -7, and -8. Further analysis of these cells revealed substantial abatement of lipid peroxidation in response to pharmacological challenge. The results suggest that the presence of ancestral GPX4 resulted in later functional divergence and specialization of GPXs in plants. The results also challenge a strict requirement for selenocysteine activity and suggest thioredoxin as a potent parallel antioxidant system in both plants and mammals.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.202000856RDOI Listing
June 2021

Comparative transcriptome analysis of leaves during early stages of chilling stress in two different chilling-tolerant brown-fiber cotton cultivars.

PLoS One 2021 9;16(2):e0246801. Epub 2021 Feb 9.

China Colored-cotton (Group) Co., Ltd., Urumqi, China.

Chilling stress generates significant inhibition of normal growth and development of cotton plants and lead to severe reduction of fiber quality and yield. Currently, little is known for the molecular mechanism of brown-fiber cotton (BFC) to respond to chilling stress. Herein, RNA-sequencing (RNA-seq)-based comparative analysis of leaves under 4°C treatment in two different-tolerant BFC cultivars, chilling-sensitive (CS) XC20 and chilling-tolerant (CT) Z1612, was performed to investigate the response mechanism. A total of 72650 unigenes were identified with eight commonly used databases. Venn diagram analysis identified 1194 differentially expressed genes (DEGs) with significant up-regulation in all comparison groups. Furthermore, enrichment analyses of COG and KEGG, as well as qRT-PCR validation, indicated that 279 genes were discovered as up-regulated DEGs (UDEGs) with constant significant increased expression in CT cultivar Z1612 groups at the dimensions of both each comparison group and treatment time, locating in the enriched pathways of signal transduction, protein and carbohydrate metabolism, and cell component. Moreover, the comprehensive analyses of gene expression, physiological index and intracellular metabolite detections, and ascorbate antioxidative metabolism measurement validated the functional contributions of these identified candidate genes and pathways to chilling stress. Together, this study for the first time report the candidate key genes and metabolic pathways responding to chilling stress in BFC, and provide the effective reference for understanding the regulatory mechanism of low temperature adaptation in cotton.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0246801PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7872267PMC
February 2021

Genome-Wide Analysis of MDHAR Gene Family in Four Cotton Species Provides Insights into Fiber Development via Regulating AsA Redox Homeostasis.

Plants (Basel) 2021 Jan 25;10(2). Epub 2021 Jan 25.

Key Laboratory of Xinjiang Phytomedicine Resource and Utilization of Ministry of Education, College of Life Sciences, Shihezi University, Shihezi 832003, China.

Monodehydroasorbate reductase (MDHAR) (EC1.6.5.4), a key enzyme in ascorbate-glutathione recycling, plays important roles in cell growth, plant development and physiological response to environmental stress via control of ascorbic acid (AsA)-mediated reduction/oxidation (redox) regulation. Until now, information regarding function and regulatory mechanism in have been limited. Herein, a genome-wide identification and comprehensive bioinformatic analysis of 36 family genes in four species, , , and , were performed, indicating their close evolutionary relationship. Expression analysis of in different cotton tissues and under abiotic stress and phytohormone treatment revealed diverse expression features. Fiber-specific expression analysis showed that , and were preferentially expressed in fiber fast elongating stages to reach peak values in 15-DPA fibers, with corresponding coincident observances of MDHAR enzyme activity, AsA content and ascorbic acid/dehydroascorbic acid (AsA/DHA) ratio. Meanwhile, there was a close positive correlation between the increase of AsA content and AsA/DHA ratio catalyzed by MDHAR and fiber elongation development in different fiber-length cotton cultivars, suggesting the potential important function of MDHAR for fiber growth. Following HO stimulation, demonstrated immediate responses at the levels of mRNA, enzyme, the product of AsA and corresponding AsA/DHA value, and antioxidative activity. These results for the first time provide a comprehensive systemic analysis of the gene family in plants and the four cotton species and demonstrate the contribution of MDHAR to fiber elongation development by controlling AsA-recycling-mediated cellular redox homeostasis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/plants10020227DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912408PMC
January 2021

Visual Detection of Alpha Toxin by Combining Nanometer Microspheres with Smart Phones.

Microorganisms 2020 Nov 26;8(12). Epub 2020 Nov 26.

School of Life Sciences, Tianjin University, Tianjin 300072, China.

α toxin (CPA) is an important virulence factor that causes livestock hemorrhagic enteritis and food poisoning by contaminated meat products. In this study, the nano-silica microspheres combined with smartphone image processing technology was developed to realize real-time CPA detection. First, the N-terminal and C-terminal domain of the CPA toxin (CPA and CPA) and their anti-sera were prepared. The silica microspheres coupled with the antibody of CPA was prepared to capture the toxin that existed in the detection sample and the fluorescent-labeled antibody of CPA was incubated. Moreover, the fluorescent pictures of gray value were performed in a cell phone app, corresponding to toxin concentration. The new assay takes 90 min to perform and can detect CPA as little as 32.8 ng/mL. Our results showed a sensitive, stable, and convenient CPA detection system, which provides a novel detection method of native CPA in foods.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/microorganisms8121865DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7761010PMC
November 2020

The antiviral protein viperin interacts with the viral N protein to inhibit proliferation of porcine epidemic diarrhea virus.

Arch Virol 2020 Oct 27;165(10):2279-2289. Epub 2020 Jul 27.

School of Life Sciences, Tianjin University, No. 92, Weijin Road, Nankai District, Tianjin, 300072, China.

In the early stage of virus infection, the pattern recognition receptor (PRR) signaling pathway of the host cell is activated to induce interferon production, activating interferon-stimulated genes (ISGs) that encode antiviral proteins that exert antiviral effects. Viperin is one of the innate antiviral proteins that exert broad-spectrum antiviral effects by various mechanisms. Porcine epidemic diarrhea virus (PEDV) is a coronavirus that causes huge losses to the pig industry. Research on early antiviral responses in the gastrointestinal tract is essential for developing strategies to prevent the spread of PEDV. In this study, we investigated the mechanisms of viperin in PEDV-infected IPEJ-C2 cells. Increased expression of interferon and viperin and decreased replication of PEDV with a clear reduction in the viral load were observed in PEDV-infected IPEC-J2 cells. Amino acids 1-50 of porcine viperin contain an endoplasmic reticulum signal sequence that allows viperin to be anchored to the endoplasmic reticulum and are necessary for its function in inhibiting PEDV proliferation. The interaction of the viperin S-adenosylmethionine domain with the N protein of PEDV was confirmed via confocal laser scanning microscopy and co-immunoprecipitation. This interaction might interfere with viral replication or assembly to reduce virus proliferation. Our results highlight a potential mechanism whereby viperin is able to inhibit PEDV replication and play an antiviral role in innate immunity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00705-020-04747-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7382991PMC
October 2020

Genome-Wide Identification and Expression Analysis of the Ascorbate Oxidase Gene Family in Reveals the Critical Role of in Delaying Dark-Induced Leaf Senescence.

Int J Mol Sci 2019 Dec 6;20(24). Epub 2019 Dec 6.

Key Laboratory of Xinjiang Phytomedicine Resource and Utilization of Ministry of Education, College of Life Sciences, Shihezi University, Shihezi 832003, China.

Ascorbate oxidase (AO) plays important roles in plant growth and development. Previously, we reported a cotton gene that acts as a positive factor in cell growth. Investigations on () family genes and their multiple functions are limited. The present study identified eight family genes and performed bioinformatic analyses. Expression analyses of the tissue specificity and developmental feature of s displayed their diverse expression patterns. Interestingly, demonstrated the most rapid significant increase in expression after 1 h of light recovery from the dark. Additionally, the transgenic / lines overexpressing in the late-flowering mutant displayed a recovery to the normal phenotype of wild-type plants. Moreover, compared to the mutant, the / transgenic presented delayed leaf senescence that was induced by the dark, indicating increased sensitivity to hydrogen peroxide (HO) under normal conditions that might be caused by a reduction in ascorbic acid (AsA) and ascorbic acid/dehydroascorbate (AsA/DHA) ratio. The results suggested that are functionally diverse in plant development and play a critical role in light responsiveness. Our study serves as a foundation for understanding the gene family in cotton and elucidating the regulatory mechanism of in delaying dark-induced leaf senescence.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijms20246167DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6940856PMC
December 2019

, the Key Gene for Ascorbate Biosynthesis in , Involves in Cell Elongation Under Control of Ethylene.

Cells 2019 09 5;8(9). Epub 2019 Sep 5.

College of Life Sciences, Key Laboratory of Xinjiang Phytomedicine Resource and Utilization of Ministry of Education, Shihezi University, Shihezi 832003, China.

L-Ascorbate (Asc) plays important roles in cell growth and plant development, and its de novo biosynthesis was catalyzed by the first rate-limiting enzyme VTC1. However, the function and regulatory mechanism of involved in cell development is obscure in . Herein, the Asc content and AsA/DHA ratio were accumulated and closely linked with fiber development. The GhVTC1 encoded a typical VTC1 protein with functional conserved domains and expressed preferentially during fiber fast elongation stages. Functional complementary analysis of in the loss-of-function mutants indicated that 1 is genetically functional to rescue the defects of mutants to normal or wild type (WT). The significant shortened primary root in mutants was promoted to the regular length of WT by the ectopic expression of in the mutants. Additionally, expression was induced by ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC), and the promoter showed high activity and included two ethylene-responsive elements (ERE). Moreover, the 5'-truncted promoters containing the ERE exhibited increased activity by ACC treatment. Our results firstly report the cotton function in promoting cell elongation at the cellular level, and serve as a foundation for further understanding the regulatory mechanism of Asc-mediated cell growth via the ethylene signaling pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/cells8091039DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6769745PMC
September 2019

miR403a and SA Are Involved in Mediated Antiviral Defenses Against TMV Infection in .

Genes (Basel) 2019 07 12;10(7). Epub 2019 Jul 12.

Key Laboratory of Forage and Endemic Crop Biotechnology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot 010070, China.

RNAi (RNA interference) is an important defense response against virus infection in plants. The core machinery of the RNAi pathway in plants include DCL (Dicer Like), AGO (Argonaute) and RdRp (RNA dependent RNA polymerase). Although involvement of these RNAi components in virus infection responses was demonstrated in , their contribution to antiviral immunity in , a model plant for plant-pathogen interaction studies, is not well understood. In this study, we investigated the role of gene against TMV (Tomato mosaic virus) infection. Silencing of by transient expression of an hpRNA construct recovered GFP (Green fluorescent protein) expression in -silenced plant, demonstrating that participated in RNAi process in . Expression of was transcriptionally induced by both MeSA (Methylsalicylate acid) treatment and TMV infection. Down-regulation of gene by amiR- transient expression compromised plant resistance against TMV infection. Inhibition of endogenous miR403a, a predicted regulatory microRNA of , reduced TMV infection. Our study provides evidence for the antiviral role of against a family virus TMV in , and SA (Salicylic acid) mediates this by induction of expression upon TMV infection. Our data also highlighted that miR403a was involved in TMV defense by regulation of target gene in .
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/genes10070526DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6679004PMC
July 2019

Screening the Reference Genes for Quantitative Gene Expression by RT-qPCR During SE Initial Dedifferentiation in Four Cultivars that Have Different SE Capability.

Genes (Basel) 2019 06 28;10(7). Epub 2019 Jun 28.

Key Laboratory of Xinjiang Phytomedicine Resource and Utilization of Ministry of Education, College of Life Sciences, Shihezi University, Shihezi 832003, China.

RNA sequencing (RNA-Seq)-based gene expression analysis is applicable to a wide range of biological purposes in various species. Reverse transcription quantitative PCR (RT-qPCR) is also used to assess target gene expression utilizing stably expressed reference genes as internal control under a given set of conditions. However, investigations of the reference genes for RT-qPCR normalization in the process of somatic embryogenesis (SE) initial dedifferentiation in are rarely reported. In this study, on the basis of our previous transcriptome data of three different induction stages during SE initial dedifferentiation process in four cultivars that have different SE capability, 15 candidate genes were selected during SE initial dedifferentiation process, and their expression stability was evaluated by geNorm, NormFinder, and BestKeeper. The results indicated that the two genes of () and () showed stable expression in the four different cultivars, endowing them to be appropriate reference genes during three induction stages in the four cotton cultivars. In addition, the stability and reliability of the two reference genes of and were further verified by comparing the expressions of () and () between RT-qPCR results and the RNA-seq data, which showed strong positive correlation coefficient (R = 0.8396-0.9984), validating again the steady expression of and as the reliable reference genes. Our results provide effective reference genes for RT-qPCR normalization during SE process in different cultivars.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/genes10070497DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6678594PMC
June 2019

Calcium-Dependent Protein Kinase Genes in Appear to be Involved in Promoting the Biosynthesis of Glycyrrhizic Acid and Flavonoids under Salt Stress.

Molecules 2019 May 13;24(9). Epub 2019 May 13.

Key Laboratory of Xinjiang Phytomedicine Resource and Utilization of Ministry of Education, College of Life Sciences, Shihezi University, Shihezi 832003, China.

As calcium signal sensors, calcium-dependent protein kinases (CPKs) play vital roles in stimulating the production of secondary metabolites to participate in plant development and response to environmental stress. However, investigations of the family genes and their multiple functions are rarely reported. In this study, a total of 23 genes in were identified, and their phylogenetic relationships, evolutionary characteristics, gene structure, motif distribution, and promoter -acting elements were analyzed. Ten showed root-specific preferential expressions, and indicated different expression patterns under treatments of CaCl and NaCl. In addition, under 2.5 mM of CaCl and 30 mM of NaCl treatments, the diverse, induced expression of and significant accumulations of glycyrrhizic acid and flavonoids suggested the possible important function of in regulating the production of glycyrrhizic acid and flavonoids. Our results provide a genome-wide characterization of family genes in , and serve as a foundation for understanding the potential function and regulatory mechanism of in promoting the biosynthesis of glycyrrhizic acid and flavonoids under salt stress.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/molecules24091837DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6539831PMC
May 2019

A Cotton () -Inositol-1-Phosphate Synthase () Gene Promotes Root Cell Elongation in .

Int J Mol Sci 2019 Mar 11;20(5). Epub 2019 Mar 11.

College of Life Sciences, Key Laboratory of Xinjiang Phytomedicine Resource and Utilization of Ministry of Education, Shihezi University, Shihezi 832003, China.

-inositol-1-phosphate synthase (MIPS, EC 5.5.1.4) plays important roles in plant growth and development, stress responses, and cellular signal transduction. genes were found preferably expressed during fiber cell initiation and early fast elongation in upland cotton (), however, current understanding of the function and regulatory mechanism of genes to involve in cotton fiber cell growth is limited. Here, by genome-wide analysis, we identified four genes anchoring onto four chromosomes in and analyzed their phylogenetic relationship, evolutionary dynamics, gene structure and motif distribution, which indicates that genes are highly conserved from prokaryotes to green plants, with further exon-intron structure analysis showing more diverse in plants. Of the four members, based on the significant accumulated expression of at the early stage of fiber fast elongating development, thereby, the was selected to investigate the function of participating in plant development and cell growth, with ectopic expression in the loss-of-function mutants. The results showed that is a functional gene to fully compensate the abnormal phenotypes of the deformed cotyledon, dwarfed plants, increased inflorescence branches, and reduced primary root lengths in mutants. Furthermore, shortened root cells were recovered and normal root cells were significantly promoted by ectopic expression of in mutant and wild-type plants respectively. These results serve as a foundation for understanding the family genes in cotton, and suggest that may function as a positive regulator for plant cell elongation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijms20051224DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6429088PMC
March 2019

Comparative Transcriptome Analysis of SE initial dedifferentiation in cotton of different SE capability.

Sci Rep 2017 08 17;7(1):8583. Epub 2017 Aug 17.

Colleges of Life Science, Shihezi University, Shihezi, China.

Somatic embryogenesis (SE) is a critical transition from vegetative to embryogenic growth in higher plants; however, few studies have investigated the mechanism that regulates SE initial differentiation. Most cotton varieties have not undergone regeneration by SE, so only a few varieties can be used in genetic engineering. Here, two varieties of cotton with different SE capabilities (HD, higher differentiation and LD, lower differentiation) were analyzed by high throughout RNA-Seq at the pre-induction stage (0h) and two induction stages (3h and 3d) under callus-induction medium (CIM). About 1150 million clean reads were obtained from 98.21% raw data. Transcriptomic analysis revealed that "protein kinase activity" and "oxidoreductase activity" were highly represented GO terms during the same and different treatment stages among HD and LD. Moreover, several stress-related transcription factors might play important roles in SE initiation. The SE-related regulation genes (SERKs) showed different expression patterns between HD and LD. Furthermore, the complex auxin and ethylene signaling pathway contributes to initiation of differentiation in SE. Thus, our RNA-sequencing of comparative transcriptome analysis will lay a foundation for future studies to better define early somatic formation in cotton with different SE capabilities.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-017-08763-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5561258PMC
August 2017

Immunization with a DNA vaccine encoding Toxoplasma gondii Superoxide dismutase (TgSOD) induces partial immune protection against acute toxoplasmosis in BALB/c mice.

BMC Infect Dis 2017 06 7;17(1):403. Epub 2017 Jun 7.

Department of Parasitology, School of Medicine, Shandong University, Jinan, Shandong Province, People's Republic of China.

Background: Toxoplasma gondii (T. gondii) is an obligate intracellular protozoan parasite that infects all warm-blooded animals including humans and causes toxoplasmosis. An effective vaccine could be an ideal choice for preventing and controlling toxoplasmosis. T. gondii Superoxide dismutase (TgSOD) might participate in affecting the intracellular growth of both bradyzoite and tachyzoite forms. In the present study, the TgSOD gene was used to construct a DNA vaccine (pEGFP-SOD).

Methods: TgSOD gene was amplified and inserted into eukaryotic vector pEGFP-C1 and formed the DNA vaccine pEGFP-SOD. Then the BALB/c mice were immunized intramuscularly with the DNA vaccine and those injected with pEGFP-C1, PBS or nothing were treated as controls. Four weeks after the last immunization, all mouse groups followed by challenging intraperitoneally with tachyzoites of T. gondii ME49 strain.

Results: Results showed higher levels of total IgG, IgG2α in the sera and interferon gamma (IFN-γ) in the splenocytes from pEGFP-SOD inoculated mice than those unvaccinated, or inoculated with either empty plasmid vector or PBS. The proportions of CD4 T cells and CD8 T cells in the spleen from pEGFP-SOD inoculated mice were significantly (p < 0.05) increased compared to control groups. In addition, the survival time of mice immunized with pEGFP-SOD was significantly prolonged as compared to the controls (p < 0.05) although all the mice died.

Conclusion: The present study revealed that the DNA vaccine triggered strong humoral and cellular immune responses, and aroused partial protective immunity against acute T. gondii infection in BALB/c mice. The collective data suggests the SOD may be a potential vaccine candidate for further development.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12879-017-2507-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5463464PMC
June 2017

Toxoplasma gondii: Vaccination with a DNA vaccine encoding T- and B-cell epitopes of SAG1, GRA2, GRA7 and ROP16 elicits protection against acute toxoplasmosis in mice.

Vaccine 2015 Nov 27;33(48):6757-62. Epub 2015 Oct 27.

Department of Parasitology, School of Medicine, Shandong University, PR China. Electronic address:

Toxoplasma gondii (T. gondii) is an obligate, intracellular, protozoan parasite that infects large variety of warm-blooded animals including humans, livestock, and marine mammals, and causes the disease toxoplasmosis. Although T. gondii infection rates differ significantly from country to country, it still has a high morbidity and mortality. In these circumstances, developing an effective vaccine against T. gondii is urgently needed for preventing and treating toxoplasmosis. The aim of this study was to construct a multi-epitopes DNA vaccine and evaluate the immune protective efficacy against acute toxoplasmosis in mice. Therefore, twelve T- and B-cell epitopes from SAG1, GRA2, GRA7 and ROP16 of T. gondii were predicted by bioinformatics analysis, and then a multi-epitopes DNA vaccine was constructed. Mice immunized with the multi-epitopes DNA vaccine gained higher levels of IgG titers and IgG2a subclass titers, significant production of gamma interferon (IFN-γ), percentage of T lymphocyte subsets, and longer survival times against the acute infection of T. gondii compared with those of mice administered with empty plasmid and those in control groups. Furthermore, a genetic adjuvant pEGFP-RANTES (pRANTES) could enhance the efficacy of the multi-epitopes DNA vaccine associating with humoral and cellular (Th1, CD8(+) T cell) immune responses. Above all, the DNA vaccine and the genetic adjuvant revealed in this study might be new candidates for further vaccine development against T. gondii infection.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.vaccine.2015.10.077DOI Listing
November 2015

Sequence Variation in Superoxide Dismutase Gene of Toxoplasma gondii among Various Isolates from Different Hosts and Geographical Regions.

Korean J Parasitol 2015 Jun 30;53(3):253-8. Epub 2015 Jun 30.

Department of Parasitology, School of Medicine, Shandong University, Ji'nan, Shandong, PR China.

Toxoplasma gondii, an obligate intracellular protozoan parasite of the phylum Apicomplexa, can infect all warm-blooded vertebrates, including humans, livestock, and marine mammals. The aim of this study was to investigate whether superoxide dismutase (SOD) of T. gondii can be used as a new marker for genetic study or a potential vaccine candidate. The partial genome region of the SOD gene was amplified and sequenced from 10 different T. gondii isolates from different parts of the world, and all the sequences were examined by PCR-RFLP, sequence analysis, and phylogenetic reconstruction. The results showed that partial SOD gene sequences ranged from 1,702 bp to 1,712 bp and A + T contents varied from 50.1% to 51.1% among all examined isolates. Sequence alignment analysis identified total 43 variable nucleotide positions, and these results showed that 97.5% sequence similarity of SOD gene among all examined isolates. Phylogenetic analysis revealed that these SOD sequences were not an effective molecular marker for differential identification of T. gondii strains. The research demonstrated existence of low sequence variation in the SOD gene among T. gondii strains of different genotypes from different hosts and geographical regions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3347/kjp.2015.53.3.253DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4510687PMC
June 2015