Publications by authors named "Aiguo Zhou"

95 Publications

Insights into carbon isotope fractionation on trichloroethene degradation in base activated persulfate process: The role of multiple reactive oxygen species.

Sci Total Environ 2021 Jul 30;800:149371. Epub 2021 Jul 30.

School of Environmental Studies, China University of Geosciences, Wuhan 430074, China.

Understanding the role of reactive oxygen species (ROS) is essential to elucidate the mechanism of contaminants degradation in in-situ chemical oxidation (ISCO). In this study, compound specific isotope analysis (CSIA) and radicals quenching methods were integrated to investigate the roles of hydroxyl radical (HO), sulfate radical (SO), and superoxide radical (O) on trichloroethene (TCE) degradation during persulfate (PS) activated with base. The carbon isotope fractionation of TCE was found to be dependent of the base:PS ratios, yielding carbon enrichment factors (ε values) from -9.8 ± 0.5‰ to -16.7 ± 1.0‰ at base:PS molar ratios between 0.5:1 and 10:1, which was attributed to multi-pathways degradation of TCE by multiple ROS. The expected ε value (-31.6 ± 1.6‰) for TCE degradation via O attacking pathway, was more negative than those values via SO or HO pathways. The relative contributions of HO, SO and O for TCE degradation during base activated PS were estimated with observed ε values. HO and O were the predominant ROS for TCE degradation (with the relative contribution of 55-69% and 22-45%, respectively) in base activated PS. This work highlights the prospect of CSIA application for identifying degradation pathways of contaminants with ROS in environment.
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http://dx.doi.org/10.1016/j.scitotenv.2021.149371DOI Listing
July 2021

Relationships between postural balance, aiming technique and performance in elite rifle shooters.

Eur J Sport Sci 2021 Sep 12:1-6. Epub 2021 Sep 12.

School of Strength and Conditioning, Beijing Sport University, Beijing, People's Republic of China.

The aim of the present study was to examine the relationships between postural balance, aiming technique and shooting score among elite rifle shooters at an intra- and inter-individual level. Twelve elite athletes belonging to China national team performed 60 shots under test conditions. Shooting score was used to indicate performance and footscan1.0 force platform was used to measure postural balance variables, while a SCATT MX-02 optoelectronic training device was used to measure aiming technique parameters. Inter-individual analyses indicated that postural balance was negatively correlated with shooting score (= -0.697, < 0.05) and aiming accuracy (= -0.810,  < 0.01). Postural balance showed a positive correlation with the stability of hold ( = 0.923,  < 0.01) and stability of triggering ( = 0.564,  < 0.05). Intra-individual analyses indicated that there was a significant correlation between postural balance and performance, aiming accuracy and stability of hold for all shooters. Postural balance was related to the stability of triggering for six shooters. Postural balance was not statistically significant with aiming time on an intra- and inter-individual basis ( > 0.05). Our data indicated that postural balance was very important in aiming technique and shooting performance among elite rifle shooters. Additional balance programmes should be encouraged to improve a shooter's postural balance.
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http://dx.doi.org/10.1080/17461391.2021.1971775DOI Listing
September 2021

Nanoplastics Induce More Serious Microbiota Dysbiosis and Inflammation in the Gut of Adult Zebrafish than Microplastics.

Bull Environ Contam Toxicol 2021 Aug 11. Epub 2021 Aug 11.

College of Marine Science, South China Agricultural University, Guangzhou, Guangdong, 510642, PR China.

Microplastics (MPs) (< 5 mm) and nanoplastics (NPs) (< 100 nm) are emerging environmental pollutants and have been proved could cause a series of toxicity in aquatic organisms. In this study, the effects on gut microbiota of adult zebrafish exposed for 21 days to 10 μg/L and 1 mg/L of MPs (8 μm) and NPs (80 nm) were evaluated. We analyzed the intestinal microbial community of zebrafish using high throughput sequencing of the 16S rRNA gene V3-V4 region and also performed transcriptional profiling of the inflammation pathway related genes in the intestinal tissues. Our results showed that both spherical polystyrene MPs and NPs could induce microbiota dysbiosis in the gut of zebrafish. The flora diversity of gut microbiota significantly increased under a high concentration of NPs. At the phylum level, the abundance of Proteobacteria increased significantly and the abundance of Fusobacteria, Firmicutes and Verrucomicrobiota decreased significantly in the gut after 21-day exposure to 1 mg/L of both MPs and NPs. Furthermore, interestingly, the abundance of Actinobacteria decreased in the MPs treatment groups but increased in the NPs treatment groups. At the genus level, revealed that the relative abundance of Aeromonas significantly increased both in the MPs and NPs treatment groups. Moreover, it was observed that NPs increased mRNA levels of il8, il10, il1β and tnfα in the gut, but not in MPs exposure group, indicating that the NPs may have a more serious effect on the gut of zebrafish than MPs to induce microbiota dysbiosis and inflammation in the gut.
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http://dx.doi.org/10.1007/s00128-021-03348-8DOI Listing
August 2021

Effects of Dexmedetomidine on Patients Undergoing Laparoscopic Surgery for Colorectal Cancer.

J Surg Res 2021 Jul 15;267:687-694. Epub 2021 Jul 15.

Department of Anesthesiology, The First People's Hospital of Changde City, Changde, China. Electronic address:

Background: Pneumoperitoneum during laparoscopic surgery has a systemic impact on the renal system and might contribute to acute kidney injury or postoperative renal dysfunction. However, effective preventive strategies are still lacking. We aimed to explore the effects of dexmedetomidine (DEX) on kidney and other organ function in patients undergoing elective laparoscopic surgery for colorectal cancer.

Materials And Methods: Fifty-six patients were randomly enrolled into the Control or DEX group. The DEX group received 1 µg kg DEX intravenously within 10 min followed by a maintenance dose of 0.5 µg kg h infused until 30 min before closing the peritoneum. In the Control group, 0.9% sodium chloride was administered as a placebo. The primary outcome was serum neutrophil gelatinase-associated lipocalin (NGAL) levels reflecting kidney injury. Secondary outcomes included variables reflecting the kidney, intestinal injury and systemic inflammatory response.

Results: NGAL levels were significantly lower in the DEX group than in the Control group at 1 d and 5 d postoperatively (107.5 ± 55.6 ng mLversus 179.5 ± 78.2 ng mL; 70.3 ± 45.8 ng mLversus 135.2 ± 59.6 ng mL, P < 0.001), while the BUN and Cr levels showed no differences between the groups. Serum DAO activity was significantly lower in the DEX group patients 24 h after surgery. Moreover, I-FABP levels were markedly lower at 2 h and 24 h postoperatively in the DEX group than in the Control group (P < 0.001).

Conclusions: Perioperative DEX administration may potentially confer kidney and intestinal protection during laparoscopic surgery for colorectal cancer patients.
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http://dx.doi.org/10.1016/j.jss.2021.06.043DOI Listing
July 2021

First Report of causing soft rot in Orchids in Canada.

Plant Dis 2021 Jun 23. Epub 2021 Jun 23.

CFIA, Canadian Food Inspection Agency, Charlottetown Laboratory, 93 Mount Edward Road, Charlottetown, Charlottetown, Prince Edward Island, Canada, C1A5T1;

Dickeya fangzhongdai was originally described as the causal agent of bleeding canker of pear tree in China. Recently, D. fangzhongdai was isolated and identified as the causal agent of soft rot in an orchid plant purchased in a local supermarket in Prince Edward Island, Canada. A water-soaked dark green spot on the leaf surface was observed and later became larger soft rot symptom. The origin of the orchid plants was traced back to a producer in Ontario, Canada who propagated them from with cuttings originally imported from the Netherlands and Taiwan. Bacterial isolations were made from a soft rot lesion on an orchid leaf by surface sterilization of small pieces of marginal tissue of the diseased leaf in 70% alcohol. The small pieces of leaf tissue were then washed three time using sterile water, and immersed in drops of sterile water. Bacterial streaming was observed under the microscope and non-fluorescing bacterial colonies were isolated on King's B and casamino acid-peptone-glucose agar plates and purified as isolates 908, 909, 910 and 911. The DNA samples were extracted from the four isolates, as well as the diseased leaf tissue, and tested by using a qPCR assay with the specific primer/probe set (DfF/DfR/DfP) for D. fangzhongdai (Tian et al. 2020). The assay yielded PCR amplicons of 135 bp with a melting temperature of 86.5±0.6 °C as did two control reactions using genomic DNA from D. fangzhongdai strains JS5T and QZH3 originally isolated in China, providing presumptive identification of the orchid isolates as D. fangzhongdai. To fulfill Koch's postulates, freshly purchased healthy orchid plants (n=4) were inoculated by leaf injection with the bacterial isolates obtained in this study and strains JS5 T and QZH3 at ~107 CFU/ml. Three leaves of the same side of the plants were inoculated with the same strains as triplicates. Sterile water was used as the negative control. Inoculated plants were incubated in a growth chamber with a 16 h photoperiod at 23 °C. Water soaked lesions developed in 3-5 days after inoculation followed by soft rotting in leaves inoculated with the new bacterial strains from orchid plants while strain QZH3 caused soft rot in 10 days after inoculation (Fig. S1). The non-fluorescing bacteria on King's B plates with colony morphology similar to those inoculated were re-isolated from the inoculated leaves and confirmed to be D. fangzhongdai by qPCR. Phylogenetic analysis of the assembled 16S rRNA sequence of isolate 908 (GenBank accession number: MT984340), together with GenBank data of all Dickeya spp. and some Pectobacterium spp, using neighbor-joining (NJ) method inferred with MEGA X software (Kumar et al. 2018) showed that isolate 908 clustered with strains JS5T and QZH3 at a phylogenetic distance of 0.0007. This clearly indicated that isolate 908 and JS5T and QZH3 belong to the same genus. Species-level identification of isolate 908 was achieved by genome sequencing and analysis based on average nucleotide identity (ANI). Genomic DNA of isolate 908 was sequenced with Illumina MiSeq to provide approximately 180X genome coverage. After quality checking using FastQC (Andrews 2010), de-novo assembly was performed with VelvetOptimiser v2.2.6 (Zerbino and Birney 2008). The draft genome size of strain 908 was 4,938,027 bp consisting of 76 contigs with 56.8% G+C content and 63,801 bp as N50. The draft genome was checked for misassembled fragments using QUAST v5.0.2 (Gurevich et al. 2013) and found to be of good quality. The draft genome sequence is deposited in GenBank under the accession number of JADCNJ000000000. The draft genome sequence of strain 908 was compared to that of D. fangzhongdai JS5T type strain genome using FastANI v1.2 (Jain et al. 2018) resulting in an ANI value of 98.9%, which is above the 95% cut-off for the same species. Previously, it was reported that D. fangzhongdai caused soft rot in orchid in Europe (Alič et al. 2018) and in onions in New York (Ma et al. 2020). The difference in virulence among D. fangzhongdai strains warrants further investigation and their pathogenicity on potato is being investigated to evaluate any threat to the potato industry. To our knowledge, this is the first report of D. fangzhongdai causing soft rot disease on orchids in Canada and North America.
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http://dx.doi.org/10.1094/PDIS-04-21-0771-PDNDOI Listing
June 2021

Value of passive anterior tibial subluxation on axial MRI in identifying anterior cruciate ligament functional deficiency in patients with advanced anteromedial osteoarthritis of the knee: a case-control study.

BMC Musculoskelet Disord 2021 May 13;22(1):434. Epub 2021 May 13.

Department of Orthopaedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.

Background: A functionally deficient anterior cruciate ligament (ACL) is considered one of the contraindications in unicompartmental knee arthroplasty (UKA). But there is still a lack of standardized and reproducible methods to assess ACL functional integrity in patients with advanced anteromedial osteoarthritis of the knee (AMOA). This study explores the value of passive anterior tibial subluxation (PATS) on axial MRI in evaluating ACL status in this population.

Methods: Patients who met UKA indications between November 2017 and September 2020 were included and grouped into "intact" (ACLI) or "deficient" (ACLD) group according to their ACL status during surgery. All participants underwent MRI with a standardized protocol. The measurements of medial and lateral PATS were conducted on axial MRI, and the mean of them was calculated as global PATS. Then the reliability and diagnostic ability of PATS were determined.

Results: A total of 85 patients (45 for ACLI group, 40 for ACLD group) were included after selection. The measurements of PATS showed excellent intra- and inter-observer reliabilities (with an intraclass correlation coefficient of at least 0.986). The global PATS of the ACLI group was significantly lower than that of the ACLD group (- 2.30 ± 1.96 vs. 1.03 ± 1.96 mm, P<0.0001). The diagnostic ability of global PATS was good (area under the curve = 0.897), and a threshold of 1.2 mm had a specificity of 100%, a sensitivity of 55%, and an accuracy of 78.82%.

Conclusion: An axial global PATS of 1.2 mm on MRI is greatly specific for identifying a functionally deficient ACL in patients with advanced AMOA.
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http://dx.doi.org/10.1186/s12891-021-04328-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120852PMC
May 2021

Description of sp. nov., a phytopathogenic actinobacterium isolated from barley seeds, causing leaf brown spot and decline.

Int J Syst Evol Microbiol 2021 May;71(5)

Canadian Food Inspection Agency (CFIA), Charlottetown Laboratory, Charlottetown, PE C1A 5T1, Canada.

is a Gram-stain-positive bacterium with eight subspecies, five of which have been redefined as different species on the basis of their genome sequence data. On the basis of the results of phylogenetic analysis of gene sequences, strains of members of the genus isolated from barley have been grouped in a separate clade from other species and subspecies of the genus . In this study, the biochemical, physiological, fatty acids and genetic characteristics of strains DM1 and DM3, which represented the barley isolates, were examined. On the basis of results from multi-locus sequence typing and other biochemical and physiological features, including colony colour, carbon source utilisation and enzyme activities, DM1 and DM3 are categorically differentiated from the aforementioned eight species and subspecies of the genus . Moreover, the results of genomic analysis reveal that the DNA G+C contents of DM1 and DM3 are 73.7 and 73.5 %, respectively, and the average nucleotide identity (ANI) values between DM1 and DM3 and other species and subspecies range from 90.4 to 92.0 %. The ANI value between DM1 and DM3 is 98.0 %. These results indicate that DM1 and DM3 are distinct from other known species and subspecies of the genus . Therefore, we propose a novel species, , with DM1 (=CFCC 16553 =LMG 31970) as the type strain.
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http://dx.doi.org/10.1099/ijsem.0.004786DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8289203PMC
May 2021

Effects of mixed antimicrobial peptide on the growth performance, antioxidant and immune responses and disease resistance of Pengze crucian carp (Carassius auratus var. Pengze).

Fish Shellfish Immunol 2021 Jul 24;114:112-118. Epub 2021 Apr 24.

Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, 510642, China. Electronic address:

Antimicrobial peptides have broad-spectrum antibacterial properties and low drug resistance, and they demonstrate great potential as antibiotic substitutes. In this study, five dietary mixed antimicrobial peptide supplement groups were set and fed to Pengze crucian carp for 10 weeks. The 6 groups were G0 (control group) and 5 additional groups: G1 (100 mg/kg), G2 (200 mg/kg), G3 (400 mg/kg), G4 (800 mg/kg) and G5 (1600 mg/kg). The results showed that the final body weight (FBW), weight gain rate (WGR) and specific growth rate (SGR) of fish in G1 and G2 were higher than those of fish in the control group, and G1 was significantly higher than G0 (P < 0.05). In addition, the FBW, WGR, and SGR of the G3 group were significantly lower than those of the G0 group. The chymotrypsin, lipase and amylase activities of G1 and G2 were significantly upregulated compared with G0 and reached peak values in G1. The activity of T-AOC and SOD in the addition group was higher (except G2 and G4) than that in the control groups, and significantly increased in G3 compared to the control group. The activity of MDA in the addition group was lower than that in the control group (p > 0.05). The expression levels of TLR-4, MYD88 and TNF-α in the three organs of the addition group were higher than those in G0 and reached the peak value in G3 (p < 0.05). Furthermore, the expression levels of TLR-4, MYD88 and TNF-α in the three organs of G3 were significantly lower than those in G0 and lower than those in the other supplemented groups. The expression levels of IL-10 and IL-11 tended to be upregulated after A. hydrophila challenge, and G3 in different organs was significantly higher than that in other supplemented groups and G0. The results of this study show that an appropriate amount of mixed antimicrobial peptides can improve the growth performance and antioxidant and immune capabilities of Pengze crucian carp and can also play a positive role in the treatment of A. hydrophila infection.
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http://dx.doi.org/10.1016/j.fsi.2021.04.017DOI Listing
July 2021

A dosage-effect assessment of acute toxicology tests of microplastic exposure in filter-feeding fish.

Fish Shellfish Immunol 2021 Jun 16;113:154-161. Epub 2021 Apr 16.

Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, 510642, China. Electronic address:

Abundant microplastics was found in aquatic ecosystem and aquatic organisms, which raised many concerns in public. Silver carp (Hypophthalmichthys molitrix), a species filter-feeding planktivorous fish, feed on particle between 4 and 85 μm in size, and the respiratory process works together with feeding mechanism when filtering plankton from water. The aim of this study was to assess the physiological response of silver carp exposed to 5 μm polystyrene microspheres during 48 h of exposure followed by 48 h of depuration through the gill histology, and oxidative stress biomarkers in intestine. The results revealed that microplastics can pass through the whole digestive tract of silver carp and be excreted by feces. Low microplastic concentration (80 μg/L) induced oxidative stress and up-regulation of TUB84 and HSP70 gene in intestine, and silver carp have ability to recover after the exposure to microplastic was removed. High microplastic concentration (800 μg/L) definitely cause significant damage to gills and intestines, in this situation, far beyond the possibility of fish own repair, and even when the threaten removed, silver carp can't recovery soon. Our studies assessed the dosage-effect relationship with physiological stress on silver carp when exposure to microplastics.
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http://dx.doi.org/10.1016/j.fsi.2021.04.010DOI Listing
June 2021

Ipsilateral patellofemoral morphological abnormalities are more severe than those of contralateral joints in patients with unilateral patellar dislocation.

Knee Surg Sports Traumatol Arthrosc 2021 Aug 9;29(8):2709-2716. Epub 2021 Apr 9.

Department of Orthopaedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.

Purpose: To determine the differences in anatomic parameters between ipsilateral dislocated knees and contralateral non-dislocated knees in patients with unilateral patellar dislocation and to identify any variations in ipsilateral knees contributing to contralateral anatomic abnormalities.

Methods: A total of 82 patients with unilateral patellar dislocation from 2016 to 2019 were retrospectively evaluated. Bilateral anatomic factors, including the tibial tubercle to trochlear groove (TT-TG) distance, lower limb rotational deformities, trochlear dysplasia, patella tilt, and patellar height, were assessed by CT.

Results: The study included 46 patients (32 females and 14 males, mean age ± SD 20.5 ± 6.8). The interobserver reliability of each parameter showed excellent agreement. The ipsilateral TT-TG distance (P = 0.004), patella tilt (P = 0.001), and patellar height (P = 0.01) were greater in the ipsilateral knees than in the contralateral knees. The lateral trochlea inclination (LTI) in the contralateral knees was larger than that in the ipsilateral knees (P = 0.022). There was a significant difference in the distribution of trochlear dysplasia of Dejour between the ipsilateral knees (dislocated side) and the contralateral knees (P = 0.036). However, bilateral femoral and/or tibial torsion, and bilateral knee joint rotation did not differ significantly. Binary logistic regression showed that only ipsilateral LTI revealed significant ORs of 8.83 (P = 0.016) and 7.64 (P = 0.018) with regard to contralateral abnormal tibial torsion and LTI, respectively.

Conclusion: In patients with unilateral patellar dislocation, the ipsilateral TT-TG distance, patella tilt, and patellar height values were larger in the ipsilateral knees than in the contralateral knees, and trochlear dysplasia was more severe in the ipsilateral joints. The risks of contralateral pathological tibial torsion and LTI were 8.8- and 7.6-fold higher, respectively, in patients with abnormal ipsilateral LTI.

Level Of Evidence: Level IV.
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http://dx.doi.org/10.1007/s00167-021-06539-2DOI Listing
August 2021

High-Performance Wearable Strain Sensor Based on [email protected] Fabric with Network Structure.

Nanomaterials (Basel) 2021 Mar 31;11(4). Epub 2021 Mar 31.

Henan Key Laboratory of Materials on Deep-Earth Engineering, School of Materials Science and Engineering, Henan Polytechnic University, Jiaozuo 454000, China.

Flexible and comfortable wearable electronics are as a second skin for humans as they can collect the physiology of humans and show great application in health and fitness monitoring. MXene TiCT have been used in flexible electronic devices for their unique properties such as high conductivity, excellent mechanical performance, flexibility, and good hydrophilicity, but less research has focused on MXene-based cotton fabric strain sensors. In this work, a high-performance wearable strain sensor composed of two-dimensional (2D) MXene d-TiCT nanomaterials and cotton fabric is reported. Cotton fabrics were selected as substrate as they are comfortable textiles. As the active material in the sensor, MXene d-TiCT exhibited an excellent conductivity and hydrophilicity and adhered well to the fabric fibers by electrostatic adsorption. The gauge factor of the [email protected] fabric strain sensor reached up to 4.11 within the strain range of 15%. Meanwhile, the sensor possessed high durability (>500 cycles) and a low strain detection limit of 0.3%. Finally, the encapsulated strain sensor was used to detect subtle or large body movements and exhibited a rapid response. This study shows that the [email protected] fabric strain sensor reported here have great potential for use in flexible, comfortable, and wearable devices for health monitoring and motion detection.
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http://dx.doi.org/10.3390/nano11040889DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8065457PMC
March 2021

Interaction of environmental eukaryotic microorganisms and fungi in the pond-cultured carps: new insights into the potential pathogenic fungi in the freshwater aquaculture.

Environ Sci Pollut Res Int 2021 Aug 20;28(29):38839-38854. Epub 2021 Mar 20.

Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China.

The quality and safety of the aquatic products have gradually become the focus of global attention. In this study, the environmental eukaryotic and fungi communities in pond-cultured grass carp (Ctenopharyngodon idellus) and the koi carp (Cyprinus carpio L.) were investigated. For comparative analysis, the alpha diversity shows that the environmental microbial abundance in the koi carp groups were higher than that in the grass carp groups, while beta diversity reveals that the differences of the microbial community composition and structures in the grass carp groups were significantly higher than those in the koi carp groups. Meanwhile, the environmental microbial diversity of grass carp groups was higher than that of koi carp groups at phylum level, but showed no significant difference at genus level. Additionally, the dominant total phyla were Opisthokonta, Stramenopiles plusAlveolates plusRhizaria, Archaeplastida, Cryptophyceae, and Centrohelida for the 18S rRNA gene and Ciliophora, Chlorophyta, and Ascomycota for the ITS2 rRNA gene in both of the two carp groups. Additionally, annotation analysis showed that the biomarkers in the grass carp groups are significantly higher than those of the koi carp groups. Furthermore, the functional prediction of Funguild showed significant difference in outputs, while similarity in trophic modes and guild types between the two carp groups. Meanwhile, the total relative abundances of animal pathogen, fungal parasite, and plant pathogen were extremely similar between the two carp groups. Surprisingly, one pathogenic fungus of genus Fusarium was identified in both the environments of two carp groups based on filtered operational taxonomic unit tables. Overall, this is the first robust report to understand the characteristics of environmental eukaryotic microorganisms and fungi in the edible and ornamental carps. Our results also provide the basic data for the prevention of fungal diseases and the healthy culture of the carps.
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http://dx.doi.org/10.1007/s11356-021-13231-yDOI Listing
August 2021

How to Evaluate and Treat Skeletally Mature Patients With Patellar Dislocation.

Arthrosc Tech 2021 Mar 8;10(3):e721-e726. Epub 2021 Feb 8.

Department of Orthopaedics, the First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Although patellar dislocation is a knee disorder prevalent in adolescence and young adults, the evaluation and treatment of patients are complex and even enigmatic. Much of the literature fails to provide a detailed description of patient management. The purpose of this study is to introduce a method for the evaluation and treatment of patellar dislocation. This Technical Note will help guide clinicians in the clinical evaluation of patients and formulation of treatment plans.
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http://dx.doi.org/10.1016/j.eats.2020.10.064DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7953235PMC
March 2021

Grassland ecological restoration based on the relationship between vegetation and its below-ground habitat analysis in steppe coal mine area.

Sci Total Environ 2021 Jul 4;778:146221. Epub 2021 Mar 4.

School of Environmental Studies, China University of Geosciences, Wuhan, Hubei 430074, China.

Coal mining activities have caused severe disturbances to the regional ecosystem and soil properties. Studying the relationship of vegetation and its below-ground habitat (RVBGH) is of great significance to the coal mine land ecological restoration. To identify the optimal thickness of the soil layer for mine land ecosystem recovery and characterize the changes of the reclaimed soil-vegetation ecosystem, we studied natural and reclaimed grassland at the coal mine of Baorixile, located in Hulunbuir Steppe, Northeastern China. The vegetation and its below-ground habitat (BGH), including "root mass" and soil properties in the soil profile (0-100 cm), were surveyed in the natural and different year-reclaimed grassland. The "root mass" is the part of root system that has the most amounts of root hairs, which is the functional zone for plant growth. The RVBGH was identified by the "root mass" and plant water extraction depth, measured through the isotope δD and δO method. Firstly, the BGH of natural grassland was identified as a "double layer" vertical structure, with the "root mass" of 37.11 ± 2.57% fine roots at 0-20 cm range and 30.44 ± 2.67% at 20-40 cm range. The annual and perennial plant "root mass" was found at 0-20 cm and 20-40 cm depth, respectively. Secondly, an optimal thickness of the soil covering of 30-40 cm was proposed for grassland reclamation. Thirdly, along the reclamation chronosequence, the values of the reclaimed vegetation properties (height, density, coverage and diversity) increased and a deep root system was established. The "root mass" increased from one layer to double layer and the amount of total fine roots increased from 1621 ± 231.9 to 4459 ± 456.0. Moreover, the reclaimed soil properties of organic matter, cation exchange capacity, available N and P also increased. The study proposed that vegetation restoration was significantly correlated with its BGH, suggesting that vegetation BGH recovery is crucial in ecological restoration.
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http://dx.doi.org/10.1016/j.scitotenv.2021.146221DOI Listing
July 2021

Prevalence and Risk Factors of Osteoporosis in Chinese Postmenopausal Women Awaiting Total Knee Arthroplasty.

Clin Interv Aging 2021 26;16:379-387. Epub 2021 Feb 26.

Department of Orthopaedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, People's Republic of China.

Background: Total knee arthroplasty (TKA) complications associated with low bone quality are challenging for orthopaedic surgeons to treat, but little is known about bone quality in Chinese postmenopausal women awaiting TKA. This study investigated the incidence of osteoporosis (OP) and explored the preoperative risk factors for OP in this population.

Methods: We retrospectively reviewed the data of Chinese postmenopausal women who were indicated for TKA between May 2017 and June 2020. The bone mineral density (BMD) of the hip and lumbar spine and multiple preoperative parameters were collected and analyzed. Binary logistic regression analysis was performed to identify independent risk factors for OP in this population.

Results: A total of 204 postmenopausal women with advanced knee OA were included in the study (age: 69.7±8.5 years; body mass index [BMI]: 25.5±4.0 kg/m2). The OP prevalence among all participants was 59.8%, and the patients aged 60-80 years had a significantly lower BMD than did the age/ethnicity-adjusted population. An age ≥60 years, a BMI<25, and the presence of a varus knee deformity were independent risk factors for preoperative OP in the postmenopausal women awaiting TKA.

Conclusion: The prevalence of OP in Chinese postmenopausal women awaiting TKA is higher than that in the age/ethnicity-adjusted normal population. An age ≥60 years, a BMI<25, and the presence of a varus knee deformity are independent risk factors that can be used to predict preoperative OP in this population.
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http://dx.doi.org/10.2147/CIA.S297947DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7924246PMC
May 2021

Identification of key biomarkers and functional pathways in osteosarcomas with lung metastasis: Evidence from bioinformatics analysis.

Medicine (Baltimore) 2021 Feb;100(6):e24471

Department of Orthopaedics.

Background: In osteosarcoma, the lung is the most common metastatic organ. Intensive work has been made to illuminate the pathogeny, but the specific metastatic mechanism remains unclear. Thus, we conducted the study to seek to find the key genes and critical functional pathways associated with progression and treatment in lung metastasis originating from osteosarcoma.

Methods: Two independent datasets (GSE14359 and GSE85537) were screened out from the Gene Expression Omnibus (GEO) database and the overlapping differentially expressed genes (DEGs) were identified using GEO2R online platform. Subsequently, the Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways enrichment analysis of DEGs were conducted using DAVID. Meanwhile, the protein-protein interaction (PPI) network constructed by STRING was visualized using Cytoscape. Afterwards, the key module and hub genes were extracted from the PPI network using the MCODE and cytoHubba plugin. Moreover, the raw data obtained from GSE73166 and GSE21257 were applied to verify the expression differences and conduct the survival analyses of hub genes, respectively. Finally, the interaction network of miRNAs and hub genes constructed by ENCORI was visualized using Cytoscape.

Results: A total of 364 DEGs were identified, comprising 96 downregulated genes and 268 upregulated genes, which were mainly involved in cancer-associated pathways, adherens junction, ECM-receptor interaction, focal adhesion, MAPK signaling pathway. Subsequently, 10 hub genes were obtained and survival analysis demonstrated SKP2 and ASPM were closely related to poor prognosis of patients with osteosarcoma. Finally, hsa-miR-340-5p, has-miR-495-3p, and hsa-miR-96-5p were found to be most closely associated with these hub genes according to the interaction network of miRNAs and hub genes.

Conclusion: The key genes and functional pathways identified in the study may contribute to understanding the molecular mechanisms involved in the carcinogenesis and progression of lung metastasis originating from osteosarcoma, and provide potential diagnostic and therapeutic targets.
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http://dx.doi.org/10.1097/MD.0000000000024471DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7886415PMC
February 2021

Interactions of microplastics and antibiotic resistance genes and their effects on the aquaculture environments.

J Hazard Mater 2021 02 12;403:123961. Epub 2020 Sep 12.

Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China; Canadian Food Inspection Agency, 93 Mount Edward Road, Charlottetown, PEI C1A 5T1, Canada; Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou 510642, China. Electronic address:

Microplastics (MPs) and antibiotic resistance genes (ARGs) have become the increasing attention and global research hotpots due to their unique ecological and environmental effects. As susceptible locations for MPs and ARGs, aquaculture environments play an important role in their enrichment and transformation. In this review, we focused on the MPs, ARGs, and the effects of their interactions on the aquaculture environments. The facts that antibiotics have been widely applied in different kinds of agricultural productions (e.g., aquaculture) and that most of antibiotics enter the water environment with rainfall and residual in the aquaculture environment have been resulting in the emergence of antibiotic resistance bacteria (ARB). Moreover, the water MPs are effective carriers of the environmental microbes and ARB, making them likely to be continuously imported into the aquaculture environments. As a result, the formation of the compound pollutions may also enter the aquatic organisms through the food chains and eventually enter the human body after a long-term enrichment. Furthermore, the compound pollutions result in the joint toxic effects on the human health and the ecological environment. In summary, this review aims to emphasize the ecological effects and the potential hazards on the aquaculture environments where interactions between MPs and ARGs results, and calls for to reduce the use of the plastic products and the antibiotics in the aquaculture environments.
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http://dx.doi.org/10.1016/j.jhazmat.2020.123961DOI Listing
February 2021

Species-specific effect of microplastics on fish embryos and observation of toxicity kinetics in larvae.

J Hazard Mater 2021 02 12;403:123948. Epub 2020 Sep 12.

Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou 510642, China. Electronic address:

Microplastics will definitely increase the potential health risks to humans through food chain, especially by commercial fishes. Here, we studied species-specific effect of microplastics on fish embryos and observed uptake, accumulation and elimination of microplastics in larvae. We chose three commercial fish species with different feeding types as our research objects. The results we found demonstrated that microplastics abundance in larvae was related with feeding type. At the same exposure concentration, the ingestion of microplastics in carnivores was lower than that in filter feeders and omnivores. In addition, omnivores were less able to remove microplastics than filter feeders. To the best of our knowledge, this is the first study to compared the differences of microplastics ingested in fishes with feeding types under laboratory conditions, and we believe that the findings will be valid evidence to explain species-specific effect of microplastics on fishes.
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http://dx.doi.org/10.1016/j.jhazmat.2020.123948DOI Listing
February 2021

Characterization and spatial distribution of microplastics in two wild captured economic freshwater fish from north and west rivers of Guangdong province.

Ecotoxicol Environ Saf 2021 Jan 30;207:111555. Epub 2020 Oct 30.

Joint Laboratory of Guangdong province and Hong Kong region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou 510642, China. Electronic address:

As a new type of pollutant, microplastics are of emerging widespread concern, while amount of research done in freshwater environments and organisms is litter compared to that in marine. Following this reality, the categories of MPs in two economic freshwater fish at 25 sites from 11 cities in the north and west rivers of Guangdong province were documented. Here, 76 individuals belong Oreochromis niloticus and Cirrhinus molitorella were investigated and microplastics were found in the GITs of 43.4% and gills of 25%. The average abundances of microplastics have significant difference between Oreochromis niloticus (0.015 items/ g) and Cirrhinus molitorella (0.031 items/g), while no difference by individual (~1.9 items). The plastics were dominated by white in color (61%), fragment in shape (67%), and lass than 1 mm in size (74%). The spatial distribution of microplastics revealed that there are significant differences between different cities in average abundances, and the highest average abundances of MPs were found in Zhanjiang city (4.25 items/individual) and Guangzhou city (0.044 items/g), respectively. Our results fully proved that the microplastics was widely ingested by wild fish species and suggested that the abundance and distribution of microplastics are positively related with the development of economy, tourism, industry, agriculture, and fishery.
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http://dx.doi.org/10.1016/j.ecoenv.2020.111555DOI Listing
January 2021

Insights Into the Albinism Mechanism for Two Distinct Color Morphs of Northern Snakehead, Through Histological and Transcriptome Analyses.

Front Genet 2020 18;11:830. Epub 2020 Sep 18.

Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, China.

The great northern snakehead () is one of the most important economic and conservational fish in China. In this study, the melanocytes in the skin of two distinct color morphs were investigated and compared through employment of the microscopic analysis, hematoxylin and eosin (H&E) and Masson Fontana staining. Our results demonstrated the uneven distribution of melanocytes with extremely low density and most of them were in the state of aging or death. Meanwhile, there was no obvious pigment layer and melanocytes distribution pattern found in the albino-type (AT), while the melanocytes were evenly distributed with abundance in the bicolor-type (BT). The transcriptome analysis through Illumina HiSeq sequencing showed that a total of 34.93 Gb Clean Data was obtained, and Q30 base percentage reached 92.66%. The BT and AT northern snakeheads transcriptome data included a total of 56,039,701 and 60,410,063 clean reads ( = 3), respectively. In gene expression analyses, the sample correlation coefficients () were ranged between 0.92 and 1.00; the contribution of PC1 and PC2 were 50.25 and 13.73% by using PCA cluster analysis, the total number of DEGs were 1024 (559 up-regulated and 465 down-regulated), and the number of annotated DEGs was 767 (COG 172, KEGG 262, GO 288, SwissProt 548, Pfam 579 and NR 765). Additionally, 46,363 ± 873 and 44,947 ± 392 single nucleotide polymorphisms (SNPs) were compiled via genetic structure analysis, respectively. Ten key pigment-related genes were screened using qRT-PCR. And all of them revealed extremely higher expression levels in the skin of BT than those of AT. This is the first study to analyze the mechanism of albino characteristics of via histology and transcriptomics, and also provide the oretical and practical support for the protection and development of germplasm resources for .
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http://dx.doi.org/10.3389/fgene.2020.00830DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7530302PMC
September 2020

Validating the Role of Tibial Tubercle-Posterior Cruciate Ligament Distance and Tibial Tubercle-Trochlear Groove Distance Measured by Magnetic Resonance Imaging in Patients With Patellar Dislocation: A Diagnostic Study.

Arthroscopy 2021 01 17;37(1):234-242. Epub 2020 Sep 17.

Department of Orthopedics, the First Affiliated Hospital of Chongqing Medical University, Chongqing, China. Electronic address:

Purpose: To (1) compare these parameters between patients with patellar dislocation and healthy individuals on magnetic resonance imaging measurements, (2) validate the diagnostic capacity of the tibial tubercle-posterior cruciate ligament (TT-PCL) distance and tibial tubercle-trochlear groove (TT-TG) distance, (3) define the pathologic threshold values of these parameters for tibial tubercle osteotomy indication, and (4) compare these values with those of previous studies.

Methods: Seventy patients with patellar dislocation and 70 healthy volunteers were identified. The inter- and intraobserver reliability values were determined using Bland-Altman analysis and the intraclass correlation coefficient (ICC). The diagnostic capacity of the parameters was evaluated using receiver operating characteristic curves and the area under the receiver operating characteristic curve. The data of the control group were used to determine the pathologic threshold values of the measurements. Logistic regression analysis was performed with these pathologic threshold values.

Results: Significant differences in the TT-PCL distance (P = .01) and TT-TG distance (P < .001) were found between the study group (21.48 ± 3.18 and 12.91 ± 3.80, respectively) and the control group (20.07 ± 2.99 and 8.46 ± 3.16, respectively). Both the TT-PCL distance and TT-TG distance had excellent inter- and intraobserver agreement, with inter-ICCs >0.915 and intra-ICCs >0.932, respectively. However, the TT-TG distance had a higher area under the receiver operating characteristic curve than did the TT-PCL distance (0.820 vs 0.627). The pathologic threshold value of the TT-PCL distance was 24.76 mm. The pathologic threshold value of the TT-TG distance was 13.64 mm. Subjects with a TT-TG distance of >13.64 mm had a greater risk for patellar dislocation, with an odds ratio of 14.02 (95% confidence interval 4.00-49.08, P < .001).

Conclusions: Both the TT-PCL distance and TT-TG distance can be measured reliably by magnetic resonance imaging; however, the TT-TG distance has a better diagnostic capacity than does the TT-PCL distance.

Level Of Evidence: Retrospective cohort study (diagnosis); Level of evidence, I.
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http://dx.doi.org/10.1016/j.arthro.2020.09.004DOI Listing
January 2021

A First Insight into the Structural and Functional Comparison of Environmental Microbiota in Freshwater Turtle at Different Growth Stages under Pond and Greenhouse Cultivation.

Microorganisms 2020 Aug 21;8(9). Epub 2020 Aug 21.

Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, Souzth China Agricultural University, Guangzhou 510642, China.

The microbial community structure of water is an important indicator for evaluating the water quality of the aquaculture environment. In this study, the investigation and comparison of the bacterial communities of pond cultivation (PC) and greenhouse cultivation (GC) between hatchling, juvenile, and adult growth stages of were performed. In addition, the V4 regions of the 16S rRNA gene were sequenced. The Chao1 richness estimator of the PC group was significantly higher than that of the GC group. The beta diversity showed that the microbiotas of the two groups were isolated from each other. The dominant phyla were Cyanobacteria, Proteobacteria, Actinobacteria, Bacteroidetes, Verrucomicrobia, and Planctomycetes in the PC group and Proteobacteria, Bacteroidetes, Firmicutes, Cyanobacteria, Chloroflexi, and Actinobacteria in the GC group. Both the numbers and the types of Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway annotations differed between the PC and GC groups. The prediction of bacterial phenotype implied that the GC environment is more likely to deteriorate, and turtles are more susceptible to pathogens than those of the PC environment. In addition, a total of nine potential pathogenic bacteria were identified and the correlation of environmental factors analyses showed significant differences of bacterial species between the PC and GC groups, while the potential pathogenic bacteria showed significant correlation with the stocking density, temperature, pH, orthophosphate (PO-P), and dissolved oxygen (DO) in both the PC and GC groups. Noticeably, this is the first report to describe the different microbiota characteristics of the different cultivation environments in the different growth stages of , which will provide valuable data for water quality adjustment, disease prevention, and the healthy breeding of turtles.
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http://dx.doi.org/10.3390/microorganisms8091277DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7564371PMC
August 2020

Groundwater environmental risk assessment of abandoned coal mine in each phase of the mine life cycle: a case study of Hongshan coal mine, North China.

Environ Sci Pollut Res Int 2020 Nov 23;27(33):42001-42021. Epub 2020 Jul 23.

School of Environmental Studies, China University of Geosciences, Wuhan, 430074, Hubei, China.

Human activities during each phase of coal mine life cycle greatly affect groundwater environment. The groundwater environment destruction is not just only the destruction of underground structure but also the social problems caused by available groundwater resources reduction, as well as the environmental problems affecting ecosystem and human health. Moreover, the groundwater environmental risk of coal mining is complex, dynamic, and long-term. Therefore, a framework and quantitative method for groundwater environmental risk analysis at different phases of the mine life cycle was presented, which is composed of the groundwater system destruction risk (GSDR) and social-economic-ecological vulnerability (SEEV) assessment. The framework was applied in Hongshan abandoned coal mine, North China. Based on the aquifer structure destruction, groundwater flow field evolution, contamination, and social-economic influence analysis, 12 main controlling factors for the GSDR and 7 factors for the SEEV were determined and quantified separately. The results showed that the groundwater contamination of the Hongshan mine mainly occurred after closure, caused by the cross-strata pollution of mine water, which significantly reduced the groundwater available resources, which greatly affected local social-economy sustainable development and residents' health. The Hongshan mine closure increased groundwater environmental risk, with the GSDR high-risk zone being 12.51 km larger than that during the mining phase and the SEEV was calculated at a high level. This framework promotes systematic integration of the groundwater environmental risk assessment in mine life cycle.
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http://dx.doi.org/10.1007/s11356-020-10056-zDOI Listing
November 2020

Occurrence and distribution of microplastics in commercial fishes from estuarine areas of Guangdong, South China.

Chemosphere 2020 Dec 11;260:127656. Epub 2020 Jul 11.

Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, 510642, China. Electronic address:

The widespread presence of microplastics in global aquatic ecosystems has aroused growing concerns regarding their potential impact on aquatic biota. Data show an extensive plastic pollution in coastal areas of Guangdong, China, however, to date there has been no quantitative study on the ingestion of microplastics by commercial fishes from the estuarine areas. In this study, we analyzed the abundance, characteristics, species-specific and spatial distribution of microplastics in six species of commercial fish from estuarine areas of Guangdong. Sixty-four fish samples from nineteen sampling sites were investigated. The average abundance of microplastics in the gastrointestinal tracts of the commercial fishes was 5.4 items/individual, indicating potential food safety. The majority of microplastics were fibers, white in color and less than 1 mm in size. Species-specific and spatial distribution of the microplastics were observed in terms of abundance, shape, color and size. Sillago japonica had the highest (6.9 items/individual), while Alepes djedaba had the lowest (3 items/individual) abundance of microplastics in the gastrointestinal tract. We recommend demersal Mugil cephalus and pelagic Konosirus punctatus to be considered as two species suitable for monitoring microplastics ingestion in the seafloor and water column communities.
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http://dx.doi.org/10.1016/j.chemosphere.2020.127656DOI Listing
December 2020

Benzo[a]pyrene induces microbiome dysbiosis and inflammation in the intestinal tracts of western mosquitofish (Gambusia affinis) and zebrafish (Danio rerio).

Fish Shellfish Immunol 2020 Oct 25;105:24-34. Epub 2020 Jun 25.

College of Marine Science, South China Agricultural University, Guangzhou, Guangdong, 510642, PR China; Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, 510642, China. Electronic address:

Benzo[a]pyrene (BaP) is one of the most well studied carcinogenic polycyclic aromatic hydrocarbons (PAHs) that has been associated with a wide range of toxic effects in aquatic organisms. In the present study, the mosquitofish and zebrafish were exposed to BaP (100 μg/L) for 15 days. We analyzed the intestinal microbial community of mosquitofish and zebrafish using 16S rRNA gene amplicon sequencing and also performed transcriptional profiling of the inflammation pathway related genes in the intestinal tissues. Our results showed that BaP exposure induced similar changes to the composition of microbial community in mosquitofish and zebrafish. At the phylum level, the abundance of Proteobacteria decreased while the abundance of Firmicutes increased following BaP exposure. At the genus level, a common pathogenic genus staphylococcus significantly increased in the BaP treatment groups, compared to the control (DMSO, ~0.001% v/v). In addition, it was observed that BaP significantly increased the mRNA level of il1β in both mosquitofish and zebrafish. The transcript levels of il6, il8, il10 and ifnphi1 were significantly increased in zebrafish, however not in mosquitofish, following Bap exposure. Our findings suggest that BaP could induce microbiota dysbiosis and inflammation in the intestine of mosquitofish and zebrafish.
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http://dx.doi.org/10.1016/j.fsi.2020.06.041DOI Listing
October 2020

Expression of HSP70 family mRNAs in albino northern snakehead, Channa argus: Response to extreme temperature stress and bacterial infection.

Fish Shellfish Immunol 2020 Sep 18;104:457-469. Epub 2020 Jun 18.

Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, 510642, China. Electronic address:

The wild albino northern snakehead, Channa argus, has only been found in Jialing Rivers System of China so far. It is easy to be affected by the environmental factors such as temperature changes and bacterial infection, thus causing a huge economic loss. Therefore, this study cloned a 2,213 bp cDNA that encodes a protein of heat shock protein 70 (CaHSP70), which has an open reading frame (ORF) that encodes 639 amino acids and the corresponding polypeptides of 70.50 kDa. And the oretical isoelectric point (pI) of CaHSP70 is 5.79. Additionally, we also cloned a cDNA for heat shock cognate protein 70 (CaHSC70) with a total length of 2,300 bp. And the ORF of CaHSC70 encodes 648 amino acids and 71.18 kDa peptides. The pI of CaHSC70 is 5.22. Moreover, the cDNA length of stress-70 protein mitochondrial (CaHSPA9) is 2,944 bp with an ORF that encodes 679 amino acids, polypeptides of 73.74 kDa, and a pI of 6.68. The quantitative real-time PCR (qRT-PCR) analysis showed that the mRNA expression levels of CaHSP70, CaHSC70, and CaHSPA9 genes were tissue-specific in the control groups. After the heat shock at 37 °C, the mRNA expression levels of CaHSP70 were extremely significantly upregulated in the kidney, liver, spleen, and brain tissues, while fewer mRNA expression levels of CaHSC70 and CaHSPA9 showed a relatively induction in these tissues. In contrast, after the cold shock at 8.5 °C, fewer mRNA expression levels of CaHSP70, CaHSC70, and CaHSPA9 showed the changes of expression in all the tissues, compared to heat shock. In addition, CaHSP70, CaHSC70, and CaHSPA9 mRNA expression levels showed an overall trend of first upregulating and then falling after Edwardsiella tarda (strain No. DL1,476) challenge. In conclusion, this study demonstrated that temperature had a great effect on the mRNA expression levels of CaHSP70, CaHSC70, and CaHSPA9, and the mRNA expression levels of these three genes were also sensitive to pathogen infection, especially CaHSP70 in the albino C. argus.
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http://dx.doi.org/10.1016/j.fsi.2020.05.033DOI Listing
September 2020

Microplastics in wild freshwater fish of different feeding habits from Beijiang and Pearl River Delta regions, south China.

Chemosphere 2020 Nov 10;258:127345. Epub 2020 Jun 10.

Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, 510642, China. Electronic address:

Microplastic pollution is a major global challenge and a severe threat to aquatic organisms. The Pearl River Basin is the most important river system in Guangdong Province, China. As the second largest tributary of the Pearl River Basin, the Beijiang River flows through many cities in Guangdong Province and eventually merges into the Pearl River Delta. The goal of this research was to explore the microplastic pollution problem in the Beijiang River and Pearl River Delta. In total, 26 surface water and 47 wild freshwater fish samples from the Pearl River Delta and the main runoff area of the Beijiang River were collected. The average abundance of microplastics in surface water samples from the Beijiang River was 3183 items/m³, and the average abundance in fish samples was 5.6 items/individual; the values for the Pearl River Delta were 7571 items/m³ and 4.8 items/individual. In all samples, fibers and fragments were the major microplastic contaminants, most were white in color, and most were <1 mm in size. The microplastic abundance in carnivorous fish samples in the Beijiang River and Pearl River Delta was 3.5 and 3 items/individual, respectively, and omnivorous fish samples had 6.8 and 6.2 items/individual, respectively. Thus, the microplastic abundance in omnivorous fish samples was about twice that of the carnivorous fish. These results reveal the quantity of microplastic pollution in the Beijiang River and the Pearl River Delta and provide an important basis for further study of microplastics in the freshwater ecosystem of the Pearl River Basin.
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http://dx.doi.org/10.1016/j.chemosphere.2020.127345DOI Listing
November 2020

Malalignment sign on knee magnetic resonance imaging: a new predictor for excessive femoral anteversion in patients with patellar dislocation.

Knee Surg Sports Traumatol Arthrosc 2021 Apr 8;29(4):1075-1082. Epub 2020 Jun 8.

Department of Orthopaedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.

Purpose: To evaluate the clinical relevance of the newly identified malalignment sign in predicting excessive femoral anteversion in patients with patellar dislocations.

Methods: A total of 55 patients with patellar dislocation who underwent surgical treatment between 2016 and 2019 were included in this study. Femoral anteversion, tibial torsion, and the femorotibial index were measured via a CT scan. The malalignment sign on the knee MRI was defined as a malalignment between the lateral side of the intercondylar fossa of the femur and the lateral intercondylar eminence of the tibial plateau.

Results: A positive malalignment sign was observed in 36 of the 55 patients. Increased femoral anteversion was significantly correlated with the number of frames with a positive malalignment sign (r = 0.511, P < 0.001). The value of femoral anteversion was significantly greater in the group with a positive malalignment sign (P = 0.02). For a femoral anteversion value of 32°, the sensitivity and specificity of the malalignment sign reached the maximal level of 89.5% and 47.2%, respectively.

Conclusion: Increased femoral anteversion correlated significantly with a positive malalignment sign on knee MRI. However, tibial torsion did not affect the malalignment sign. A positive malalignment sign is evidence for femoral derotation osteotomy.

Level Of Evidence: Level IV.
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http://dx.doi.org/10.1007/s00167-020-06080-8DOI Listing
April 2021

Cytochrome P450 1A mRNA in the Gambusia affinis and Response to Several PAHs.

Biochem Genet 2020 Aug 5;58(4):551-565. Epub 2020 Jun 5.

College of Marine Science, South China Agricultural University, Guangzhou, 510642, Guangdong, People's Republic of China.

Cytochrome P4501A (CYP1A) has been used as a specific biomarker for monitoring water contamination such as PAHs, PCBs and dioxins. In the present study, the cyp1a gene of Gambusia affinis was cloned and sequenced and their expressions under PAHs exposure were characterized. The newly identified cyp1a encodes a protein with 521 amino acids that shared 96-80% identity with other Cyprinodontiformes fishes. RT-PCR analysis revealed that the basal mRNA level of cyp1a was highly expressed in liver and intestine. The expression level of cyp1a was significantly induced by exposure to 100 μg/L 3, 4-Benzopyrene (BaP) for 5 days in the muscle, testis, brain, liver and intestine of adult male fish. Except in the testis, the induced mRNA level of cyp1a ultimately decreased after prolonging the exposure time to 25 days. As for testis, the induced mRNA level of cyp1a was maintained at a high level during the entire exposure time under 100 μg/L BaP exposure. Furthermore, the expression of cyp1a increased with exposure time under a relatively low exposure concentrations 1 μg/L. Regarding the effects of other PAHs, D(a,h)A, BbF, and BaA showed a statistically significant effect of induction on mRNA level of cyp1a in the muscle, testis, brain, liver and intestine.
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http://dx.doi.org/10.1007/s10528-020-09955-0DOI Listing
August 2020

Microplastic pollution in surface water from east coastal areas of Guangdong, South China and preliminary study on microplastics biomonitoring using two marine fish.

Chemosphere 2020 Oct 24;256:127202. Epub 2020 May 24.

Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, 510642, China. Electronic address:

Microplastic pollution, one of the major global challenges, is a severe threaten to aquatic organisms. The purpose of this study was to investigate the abundance, distribution and characteristics of microplastics in coastal surface waters from eastern Guangdong, south China. We chose pelagic Konosirus punctatus and demersal Mugil cephalus from different sampling sites as candidate monitoring species for the ingestion of marine microplastics, and talked about the relationship between water samples and fish samples. We tried to find out the regulation about microplastics characteristics in fish samples and fish own traits. The mean abundance of microplastics in surface water was 8895 items/m, small white fragments were dominating character. We need to increase the amount of fish data to further explore. This study would not only provide a detailed reference for a better understanding of microplastic pollution in Guangdong coastal areas, but also shed an insight in prospect of using fish as biomonitoring of microplastic wastes.
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http://dx.doi.org/10.1016/j.chemosphere.2020.127202DOI Listing
October 2020
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