Publications by authors named "Ai-Qin Wang"

43 Publications

Genome-wide identification and evolutionary analysis of RLKs involved in the response to aluminium stress in peanut.

BMC Plant Biol 2021 Jun 21;21(1):281. Epub 2021 Jun 21.

National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning, 530004, China.

Background: As an important cash crop, the yield of peanut is influenced by soil acidification and pathogen infection. Receptor-like protein kinases play important roles in plant growth, development and stress responses. However, little is known about the number, location, structure, molecular phylogeny, and expression of RLKs in peanut, and no comprehensive analysis of RLKs in the Al stress response in peanuts have been reported.

Results: A total of 1311 AhRLKs were identified from the peanut genome. The AhLRR-RLKs and AhLecRLKs were further divided into 24 and 35 subfamilies, respectively. The AhRLKs were randomly distributed across all 20 chromosomes in the peanut. Among these AhRLKs, 9.53% and 61.78% originated from tandem duplications and segmental duplications, respectively. The ka/ks ratios of 96.97% (96/99) of tandem duplication gene pairs and 98.78% (646/654) of segmental duplication gene pairs were less than 1. Among the tested tandem duplication clusters, there were 28 gene conversion events. Moreover, all total of 90 Al-responsive AhRLKs were identified by mining transcriptome data, and they were divided into 7 groups. Most of the Al-responsive AhRLKs that clustered together had similar motifs and evolutionarily conserved structures. The gene expression patterns of these genes in different tissues were further analysed, and tissue-specifically expressed genes, including 14 root-specific Al-responsive AhRLKs were found. In addition, all 90 Al-responsive AhRLKs which were distributed unevenly in the subfamilies of AhRLKs, showed different expression patterns between the two peanut varieties (Al-sensitive and Al-tolerant) under Al stress.

Conclusions: In this study, we analysed the RLK gene family in the peanut genome. Segmental duplication events were the main driving force for AhRLK evolution, and most AhRLKs subject to purifying selection. A total of 90 genes were identified as Al-responsive AhRLKs, and the classification, conserved motifs, structures, tissue expression patterns and predicted functions of Al-responsive AhRLKs were further analysed and discussed, revealing their putative roles. This study provides a better understanding of the structures and functions of AhRLKs and Al-responsive AhRLKs.
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http://dx.doi.org/10.1186/s12870-021-03031-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8215822PMC
June 2021

Transcriptome analysis reveals significant difference in gene expression and pathways between two peanut cultivars under Al stress.

Gene 2021 May 23;781:145535. Epub 2021 Feb 23.

National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning 530004, PR China; Guangxi Key Laboratory for Agro-Environment and Agro-Product Safety, Nanning 530004, PR China. Electronic address:

Aluminum (Al) toxicity is an important factor in limiting peanut growth on acidic soil. The molecular mechanisms underlying peanut responses to Al stress are largely unknown. In this study, we performed transcriptome analysis of the root tips (0-1 cm) of peanut cultivar ZH2 (Al-sensitive) and 99-1507 (Al-tolerant) respectively. Root tips of peanuts that treated with 100 μM Al for 8 h and 24 h were analyzed by RNA-Seq, and a total of 8,587 differentially expressed genes (DEGs) were identified. GO and KEGG pathway analysis excavated a group of important Al-responsive genes related to organic acid transport, metal cation transport, transcription regulation and programmed cell death (PCD). These homologs were promising targets to modulate Al tolerance in peanuts. It was found that the rapid transcriptomic response to Al stress in 99-1507 helped to activate effective Al tolerance mechanisms. Protein and protein interaction analysis indicated that MAPK signal transduction played important roles in the early response to Al stress in peanuts. Moreover, weighted correlation network analysis (WGCNA) identified a predicted EIL (EIN3-like) gene with greatly increased expression as an Al-associated gene, and revealed a link between ethylene signaling transduction and Al resistance related genes in peanut, which suggested the enhanced signal transduction mediated the rapid transcriptomic responses. Our results revealed key pathways and genes associated with Al stress, and improved the understanding of Al response in peanut.
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http://dx.doi.org/10.1016/j.gene.2021.145535DOI Listing
May 2021

Complete chloroplast genome sequence of , an important traditional Chinese medicine plant.

Mitochondrial DNA B Resour 2019 Nov 22;4(2):4163-4165. Epub 2019 Nov 22.

College of Life Sciences, Leshan Normal University, Leshan, China.

is a leguminous plant with high root yield and starch content. It is also a medicinal material in the Chinese pharmacopeia. However, the raw materials of . are often confused with some non-medicinal Pueraria plants. To enrich the genetic resources of . and guide its molecular identification, the complete chloroplast genome was sequenced and reported. The total genome of . is 153,434 bp in length. consisting of two inverted repeat regions (IR, 25,640 bp each) separated by a large single-copy (LSC, 84,155 bp) and a small single-copy region (SSC, 17,999 bp). The overall GC content is 35.41%. It contains 130 genes, including 85 protein coding genes, 8 rRNA genes and 37 tRNA genes. Phylogenetic analysis showed that . could be distinguished from other plants and closely related to the legume . This study enriches the genetic information of . and contributes to the screening of excellent germplasm.
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http://dx.doi.org/10.1080/23802359.2019.1693301DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7707730PMC
November 2019

Remarkable active-site dependent HO promoting effect in CO oxidation.

Nat Commun 2019 Aug 23;10(1):3824. Epub 2019 Aug 23.

CAS Key Laboratory of Science and Technology on Applied Catalysis, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 116023, Dalian, China.

The interfacial sites of supported metal catalysts are often critical in determining their performance. Single-atom catalysts (SACs), with every atom contacted to the support, can maximize the number of interfacial sites. However, it is still an open question whether the single-atom sites possess similar catalytic properties to those of the interfacial sites of nanocatalysts. Herein, we report an active-site dependent catalytic performance on supported gold single atoms and nanoparticles (NPs), where CO oxidation on the single-atom sites is dramatically promoted by the presence of HO whereas on NPs' interfacial sites the promoting effect is much weaker. The remarkable HO promoting effect makes the Au SAC two orders of magnitude more active than the commercial three-way catalyst. Theoretical studies reveal that the dramatic promoting effect of water on SACs originates from their unique local atomic structure and electronic properties that facilitate an efficient reaction channel of CO + OH.
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http://dx.doi.org/10.1038/s41467-019-11871-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6707188PMC
August 2019

Mechanisms by which fibroblast growth factor 20 improves motor performance in a mouse model of Parkinson's disease.

Neural Regen Res 2019 Aug;14(8):1438-1444

Qilu Hospital of Shandong University, Jinan, Shandong Province, China.

Genome-wide studies have reported that Parkinson's disease is associated with abnormal expression of various growth factors. In this study, male C57BL/6 mice aged 10 weeks were used to establish Parkinson's disease models using an intraperitoneal injection of 60 mg/kg 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine. 28 days later, 10 or 100 ng fibroblast growth factor 20 was injected intracerebroventricularly. The electrophysiological changes in the mouse hippocampus were recorded using a full-cell patch clamp. Expression of Kv4.2 in the substantia nigra was analyzed using a western blot assay. Serum malondialdehyde levels were analyzed by enzyme-linked immunosorbent assay. The motor coordination of mice was evaluated using the rotarod test. The results showed that fibroblast growth factor 20 decreased A-type potassium current in neurons of the substantia nigra, increased long-term potentiation amplitude in the hippocampus, and downregulated Kv4.2 expression. A high dose of fibroblast growth factor 20 reduced serum malondialdehyde levels and enhanced the motor coordination of mice. These findings confirm that fibroblast growth factor 20 has a therapeutic effect on the toxicity induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine, and its mechanism of action is associated with the inhibition of A-type K currents and Kv4.2 expression. All animal procedures were approved by the Animal Care and Use Committee of Qilu Hospital of Shandong University, China in 2017 (approval No. KYLL-2017-0012).
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http://dx.doi.org/10.4103/1673-5374.253527DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6524521PMC
August 2019

Relationships among mutation status, expression of RAS pathway signaling molecules, and clinicopathological features and prognosis of patients with colorectal cancer.

World J Gastroenterol 2019 Feb;25(7):808-823

Department of Medical Oncology, the Affiliated Tumor Hospital of Zhengzhou University, Zhengzhou 450008, Henan Province, China.

Background: The RAS/RAF/MEK/ERK and PI3K/AKT/mTOR signaling pathways all belong to mitogen-activated protein kinase (MAPK) signaling pathways, Mutations in any one of the upstream genes (such as the gene or the gene) may be transmitted to the protein through transcription or translation, resulting in abnormal activation of the signaling pathway. This study investigated the relationship between the gene mutation and the clinicopathological features and prognosis of colorectal cancer (CRC), and the effect of mutations on its associated proteins in CRC, with an aim to clarify the cause of tumor progression and drug resistance caused by mutation of the gene.

Aim: To investigate the gene and RAS pathway signaling molecules in CRC and to analyze their relationship with clinicopathological features and prognosis.

Methods: Colorectal cancer tissue specimens from 196 patients were analyzed for mutations using quantitative polymerase chain reaction and for KRAS, BRAF, MEK, and ERK protein expression levels using immunohistochemistry of tumor microarrays. To analyze differences of RAS pathway signaling molecule expression levels in different gene status, the relationships between these parameters and clinicopathological features, 4-year progression-free survival, and overall survival were analyzed by independent sample test, Kaplan-Meier plots, and the log-rank test. Predictors of overall and disease-free survival were assessed using a Cox proportional hazards model.

Results: Of the 196 patients, 62 (32%) carried mutations in codon 12 (53/62) or codon 13 (9/62) in exon 2 of the gene. KRAS, BRAF, ERK, and MEK protein expression was detected in 71.4%, 78.8%, 64.3%, and 50.8% of CRC tissues, respectively. There were no significant differences between mutation status and KRAS, BRAF, MEK, or ERK protein levels. Positive expression of KRAS and ERK was associated with poor tumor differentiation, and KRAS expression was also associated with age < 56 years. MEK expression was significantly associated with distant metastasis ( < 0.05). The 4-year progression-free survival rate, but not overall survival rate, was significantly higher in patients with KRAS-negative tumors than in those with KRAS-positive tumors ( < 0.05), whereas BRAF, MEK, and ERK expression was unrelated to survival. Multivariate analysis showed that only the expression of KRAS protein was a risk factor for tumor recurrence ( < 0.05). No other clinicopathological factors correlated with KRAS, BRAF, MEK, or ERK expression.

Conclusion: gene mutations do not affect downstream protein expression in CRC. KRAS protein is associated with poor tumor differentiation, older age, and a risk of tumor recurrence.
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http://dx.doi.org/10.3748/wjg.v25.i7.808DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6385012PMC
February 2019

Non defect-stabilized thermally stable single-atom catalyst.

Nat Commun 2019 01 16;10(1):234. Epub 2019 Jan 16.

State Key Laboratory of Catalysis, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.

Surface-supported isolated atoms in single-atom catalysts (SACs) are usually stabilized by diverse defects. The fabrication of high-metal-loading and thermally stable SACs remains a formidable challenge due to the difficulty of creating high densities of underpinning stable defects. Here we report that isolated Pt atoms can be stabilized through a strong covalent metal-support interaction (CMSI) that is not associated with support defects, yielding a high-loading and thermally stable SAC by trapping either the already deposited Pt atoms or the PtO units vaporized from nanoparticles during high-temperature calcination. Experimental and computational modeling studies reveal that iron oxide reducibility is crucial to anchor isolated Pt atoms. The resulting high concentrations of single atoms enable specific activities far exceeding those of conventional nanoparticle catalysts. This non defect-stabilization strategy can be extended to non-reducible supports by simply doping with iron oxide, thus paving a new way for constructing high-loading SACs for diverse industrially important catalytic reactions.
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http://dx.doi.org/10.1038/s41467-018-08136-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6335577PMC
January 2019

Effect of Group Density on the Physiology and Aggressive Behavior of Male Brandt's Voles ().

Zool Stud 2018 18;57:e35. Epub 2018 Jul 18.

College of Bioscience and Biotechnology, Yangzhou University, 48 East Wenhui Road, Yangzhou 225009, P.R. China.

Population density is well known to influence animal physiology and behavior. How population density affects the aggressive behavior of the Brandt's vole () is, however, little known. The aim of this study was to investigate the effect of group density on physiologic responses and aggressive behavior of male Brandt's voles and their potential underlying neuro-mechanism. The results show that increasing group density led to elevated serum corticosterone levels and increased spleen weight; it also induced more male-male aggressive behavior. By contrast, it had a negative effect on body growth and the weight of testis and epididymis. Aging also increased male-male aggressive behavior. Higher density reduced mRNA levels of tryptophan hydroxylase 2 (), 5-hydroxytryptamine receptor 1A (), and 5-hydroxytryptamine receptor 1B () in the amygdala and the dorsal raphe nucleus (DRN). Our results demonstrate that higher population density can intensify stress reactions and male-male aggressive behavior in Brandt's voles at the price of inhibiting body growth and reproduction. Serotonergic systems in the amygdala and the DRN may take part in the control of aggressive behavior among male voles. Our results provide novel insights into the neuro-mechanism underlying the influence of population density on aggressive behavior in Brandt's vole, and imply that aggressive behavior may play an important role in the population fluctuation of the animal.
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http://dx.doi.org/10.6620/ZS.2018.57-35DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6517712PMC
July 2018

[Effects and Mechanisms of In-situ Cement Solidification/Stabilization on a Pb-, Zn-, and Cd-Contaminated Site at Baiyin, China].

Huan Jing Ke Xue 2017 Sep;38(9):3897-3906

Beijing No.2 Middle School, Beijing 100010, China.

In order to evaluate the effects and mechanism of in-situ cement solidification/stabilization (S/S) on heavy metal contaminated soils, leaching tests, speciation analysis, and microscopic analysis were conducted after cement treatment of a Pb, Zn, and Cd contaminated site in Baiyin, China. The leaching test results showed that cement could effectively stabilize Cd and Zn, which could reduce 99.5%-100% and 96.6%-98.8% of HSO-HNO extractable Cd and Zn, respectively. However, the leaching concentration of Pb was 2.6-5.8 times higher than that before 5% cement treatment. After adding 8% cement, HSO-HNO extractable Cd and Zn were reduced by 99.6%-100% and 94.4%-97.9% respectively. Similarly, the leaching concentration of Pb was 1.9-12.5 times higher than that before 8% cement treatment. The results of sequential extraction test proposed by the European Community Bureau of Reference (BCR) showed that cement could transform acid extractable Cd and Zn to residual form and transform reducible fractions of Pb to oxidizable and residual form, increasing the stability of heavy metals in soil. The microscopic analysis results showed that Pb, Zn, and Cd could participate in the process of cement hydration and form silicate minerals and hydroxides. In conclusion, cement could be an effective S/S agent to remediate heavy metal contaminated soils, and site characteristics and environmental conditions should also be considered during the construction process.
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http://dx.doi.org/10.13227/j.hjkx.201703018DOI Listing
September 2017

Nitric Oxide Inhibits Al-Induced Programmed Cell Death in Root Tips of Peanut ( L.) by Affecting Physiological Properties of Antioxidants Systems and Cell Wall.

Front Physiol 2017 21;8:1037. Epub 2017 Dec 21.

College of Agronomy, Guangxi University, Nanning, China.

It has been reported that nitric oxide (NO) is a negative regulator of aluminum (Al)-induced programmed cell death (PCD) in peanut root tips. However, the inhibiting mechanism of NO on Al-induced PCD is unclear. In order to investigate the mechanism by which NO inhibits Al-induced PCD, the effects of co-treatment Al with the exogenous NO donor or the NO-specific scavenger on peanut root tips, the physiological properties of antioxidants systems and cell wall (CW) in root tip cells of NO inhibiting Al-induced PCD were studied with two peanut cultivars. The results showed that Al exposure induced endogenous NO accumulation, and endogenous NO burst increased antioxidant enzyme activity in response to Al stress. The addition of NO donor sodium nitroprusside (SNP) relieved Al-induced root elongation inhibition, cell death and Al adsorption in CW, as well as oxidative damage and ROS accumulation. Furthermore, co-treatment with the exogenous NO donor decreased MDA content, LOX activity and pectin methylesterase (PME) activity, increased xyloglucan endotransglucosylase (XET) activity and relative expression of the xyloglucan endotransglucosylase/hydrolase () gene. Taken together, exogenous NO alleviated Al-induced PCD by inhibiting Al adsorption in CW, enhancing antioxidant defense and reducing peroxidation of membrane lipids, alleviating the inhibition of Al on root elongation by maintaining the extensibility of CW, decreasing PME activity, and increasing XET activity and relative expression of CW.
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http://dx.doi.org/10.3389/fphys.2017.01037DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5742856PMC
December 2017

DNA methylation assay for colorectal carcinoma.

Cancer Biol Med 2017 Feb;14(1):42-49

The Affiliated Hospital of Qingdao University, Qingdao 266003, China.

Colorectal carcinoma (CRC) is a common cause of morbidity and mortality worldwide. Two pathogenic pathways are involved in the development of adenoma to CRC. The first pathway involves characterized by chromosomal instability resulting in the accumulation of mutations. The second pathway is characterized by lesions in. Aberrant DNA methylation in selected gene promoters has emerged as a new epigenetic pathway in CRC development. CRC screening is the most efficient strategy to reduce death. Specific DNA methylation events occur in multistep carcinogenesis. Epigenetic gene silencing is a causative factor of CRC development. DNA methylations have been extensively examined in stool from CRC and precursor lesions. Many methylated genes have been described in CRC and adenoma, although no definite DNA methylation biomarkers panel has been established. Multiple DNA methylation biomarkers, including secreted frizzled-related protein 2, secreted frizzled-related protein 1, tissue factor pathway inhibitor 2, vimentin, and methylguanine DNA methyltransferase, have been further investigated, and observations have revealed that DNA methylation biomarkers exhibit with high sensitivity and specificity. These markers may also be used to diagnose CRC and adenoma in early stages. Real time polymerase chain reaction (qPCR) is sensitive, scalable, specific, reliable, time saving, and cost effective. Stool exfoliated markers provide advantages, including sensitivity and specificity. A stool qPCR methylation test may also be an enhanced tool for CRC and adenoma screening.
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http://dx.doi.org/10.20892/j.issn.2095-3941.2016.0082DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5365180PMC
February 2017

Effect of photoperiod and 6-methoxybenzoxazolinone (6-MBOA) on the reproduction of male Brandt's voles (Lasiopodomys brandtii).

Gen Comp Endocrinol 2017 05 6;246:1-8. Epub 2017 Mar 6.

College of Bioscience and Biotechnology, Yangzhou University, 48 East Wenhui Road, Yangzhou 225009, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, PR China. Electronic address:

Plant secondary metabolite 6-methoxybenzoxazolinone (6-MBOA) has been suggested to stimulate animal reproduction. 6-MBOA is detected in Leymus chinensis, a main diet of Brandt's vole (Lasiopodomys brandtii). We have previously reported a stimulatory effect of 6-MBOA on reproduction of male Brandt's voles under a short-day photoperiod. The goal of this study was to investigate the effect of 6-MBOA on reproductive physiology of male Brandt's voles under a long-day photoperiod and examine if 6-MBOA under this photoperiodic regime altered the reproductive status of male Brandt's voles differently than the short-day photoperiod. Under the long-day photoperiod, a high dose of 6-MBOA decreased KiSS-1 mRNA in the arcuate nucleus (ARC), and we also saw a decrease in circulating levels of luteinizing hormone (LH), follicle-stimulating hormone (FSH), and testosterone (T). Steroidogenic acute regulatory protein (StAR) and cytochrome P450 (CYP11a1) in the testes, and relative testis weight also decreased with 6-MBOA administration. Compared to the short-day photoperiod, animals under the long-day photoperiod exhibited increased body weight as well as all other reproductive parameters. Our results showed that 6-MBOA inhibited the reproduction of male Brandt's vole under a long-day photoperiod, a stark contrast from its stimulatory effects under a short-day photoperiod. The paradoxical effects of 6-MBOA suggest it may act as a partial agonist of melatonin. These results provide insight into the complex interactions between environmental factors such as photoperiod and diet in the control of Brandt's vole reproduction.
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http://dx.doi.org/10.1016/j.ygcen.2017.03.003DOI Listing
May 2017

Reproductive responses of male Brandt's voles (Lasiopodomys brandtii) to 6-methoxybenzoxazolinone (6-MBOA) under short photoperiod.

Naturwissenschaften 2016 Apr 3;103(3-4):29. Epub 2016 Mar 3.

College of Bioscience and Biotechnology, Yangzhou University, 48 East Wenhui Road, Yangzhou, 225009, People's Republic of China.

The plant secondary metabolite 6-methoxybenzoxazolinone (6-MBOA) can stimulate and enhance animal reproduction. This compound has been successfully detected in Leymus chinensis, which is the main diet of Brandt's voles. The aim of this study was to investigate the effect of different 6-MBOA doses on the reproductive physiology of male Brandt's voles under a short photoperiod. The results showed that 6-MBOA administration increased relative testis weight, regardless of the dose, but it had little effect on the body mass. Low and middle doses of 6-MBOA increased the concentrations of luteinizing hormone and testosterone in the serum and the mRNA levels of StAR and CYP11a1 in the testes. However, 6-MBOA did not cause any significant increase in the mRNA levels of KiSS-1, GPR54, and GnRH compared to those in the control group. The mRNA level of KiSS-1 in the arcuate nucleus (ARC) was higher than that in the anteroventral periventricular nucleus (AVPV). Collectively, our results demonstrated that the number of KiSS-1-expressing neurons located in the ARC was the highest, and that 6-MBOA, which might modulate the reproductive activity along the hypothalamic-pituitary-gonadal axis, had a dose-dependent stimulatory effect on the reproductive activity of Brandt's voles under a short photoperiod. Our study provided insights into the mechanism of 6-MBOA action and the factors influencing the onset of reproduction in Brandt's voles.
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http://dx.doi.org/10.1007/s00114-016-1347-2DOI Listing
April 2016

Serum/plasma microRNAs as biomarkers for HBV-related hepatocellular carcinoma in China.

Biomed Res Int 2015 22;2015:965185. Epub 2015 Jan 22.

Department of Microbiology, Fourth Military Medical University, Xi'an, Shaanxi 710032, China.

MicroRNAs (miRNAs) are a group of small RNAs with a fundamental role in the regulation of gene expression. These RNAs have been shown to participate in various cellular and physiological processes, including cellular development, apoptosis, proliferation, and differentiation. Aberrant expression of several miRNAs was found to be involved in a large variety of neoplasms, including hepatocellular carcinoma (HCC). Previous studies have shown the existence of a large amount of stable miRNAs in human serum/plasma, which laid the foundation for studying the role of serum/plasma miRNAs in the diagnosis and prognosis of HCC. Here, we review the recent progress in research on serum miRNAs as biomarkers for HCC in Chinese patients.
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http://dx.doi.org/10.1155/2015/965185DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4320924PMC
November 2015

Aluminum induces rapidly mitochondria-dependent programmed cell death in Al-sensitive peanut root tips.

Bot Stud 2014 Dec 3;55(1):67. Epub 2014 Sep 3.

College of Agronomy, Guangxi University, Daxue Road 100, Nanning, 530004, PR, China.

Background: Although many studies suggested that aluminum (Al) induced programmed cell death (PCD) in plants, the mechanism of Al-induced PCD and its effects in Al tolerance is limited. This study was to investigate the mechanism and type of Al induced PCD and the relationship between PCD and Al tolerance.

Results: In this study, two genotypes of peanut 99-1507 (Al tolerant) and ZH2 (Al sensitive) were used to investigate Al-induced PCD. Peanut root growth inhibition induced by AlCl was concentration and time-dependent in two peanut varieties. AlCl at 100 μM could induce rapidly peanut root tip PCD involved in DNA cleavage, typical apoptotic chromatin condensation staining with DAPI, apoptosis related gene Hrs203j expression and cytochrome C (Cyt c) release from mitochondria to cytosol. Caspase3-like protease was activated by Al; it was higher in ZH2 than in 99-1507. Al increased the opening of mitochondrial permeability transition pore (MPTP), decreased inner membrane potential (ΔΨ) of mitochondria. Compared with the control, Al stress increased O and HO production in mitochondria. Reactive oxygen species (ROS) burst was produced at Al treatment for 4 h.

Conclusions: Al-induced PCD is earlier and faster in Al-sensitive peanut cultivar than in Al-tolerant cultivar. There is a negative relationship between PCD and Al resistance. Mitochondria- dependence PCD was induced by Al and ROS was involved in this process. The mechanism can be explained by the model of acceleration of senescence under Al stress.
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http://dx.doi.org/10.1186/s40529-014-0067-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5432755PMC
December 2014

Superficial siderosis of the central nervous system presenting with dysuria initially.

Clin Neurol Neurosurg 2014 Jun 13;121:77-8. Epub 2014 Mar 13.

Department of Neurology, Qilu Hospital of Shandong University, Jinan, Shandong 250012, PR China.

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http://dx.doi.org/10.1016/j.clineuro.2014.02.026DOI Listing
June 2014

Aluminum-induced programmed cell death promoted by AhSAG, a senescence-associated gene in Arachis hypoganea L.

Plant Sci 2013 Sep 29;210:108-17. Epub 2013 May 29.

College of Agronomy, Guangxi University, Nanning 530004, PR China.

Programmed cell death (PCD) is a foundational cellular process in plant development and elimination of damaged cells under environmental stresses. In this study, Al induced PCD in two peanut (Arachis hypoganea L.) cultivars Zhonghua 2 (Al-sensitive) and 99-1507 (Al-tolerant) using DNA ladder, TUNEL detection and electron microscopy. The concentration of Al-induced PCD was lower in Zhonghua 2 than in 99-1507. AhSAG, a senescence-associated gene was isolated from cDNA library of Al-stressed peanut with PCD. Open reading frame (ORF) of AhSAG was 474bp, encoding a SAG protein composed of 157 amino acids. Compared to the control and the antisense transgenic tobacco plants, the fast development and blossom of the sense transgenic plants happened to promote senescence. The ability of Al tolerance in sense transgenic tobacco was lower than in antisense transgenic tobacco according to root elongation and Al content analysis. The expression of AhSAG-GFP was higher in sense transgenic tobacco than in antisense transgenic tobacco. Altogether, these results indicated that there was a negative relationship between Al-induced PCD and Al-resistance in peanut, and the AhSAG could induce or promote the occurrence of PCD in plants.
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http://dx.doi.org/10.1016/j.plantsci.2013.05.012DOI Listing
September 2013

One-step in situ fabrication of a granular semi-IPN hydrogel based on chitosan and gelatin for fast and efficient adsorption of Cu2+ ion.

Colloids Surf B Biointerfaces 2013 Jun 23;106:51-9. Epub 2013 Jan 23.

Center for Eco-material and Green Chemistry, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China.

The novel granular semi-IPN hydrogels were in situ prepared in an aqueous solution by the free-radical grafting and crosslinking reactions among chitosan (CTS), acrylic acid (AA), gelatin (GE) and N,N'-methylene-bis-acrylamide. The FTIR spectra and elemental analysis confirmed that the AA monomers were grafted onto CTS backbone, and the GE macromolecular chains interpenetrated through the CTS-g-PAA network. The hydrogels are granular, which are composed of numerous micro-spheres according to the scanning electron microscope observations. The gel strength, adsorption, reuse and recovery properties of the hydrogels for Cu(2+) ion were systematically investigated. The results indicate the hydrogel with 2 wt% GE has the highest adsorption capacity of 261.08 mg/g with the recovery ratio of 95.2%. And the incorporation of 10 wt% GE enhanced the storage modulus by 103.4% (ω=100 rad/s) and 115.1% (ω=0.1 rad/s), and the adsorption rate by 5.67%. Moreover, the adsorption capacity of the hydrogel is still as high as 153.9 mg/g, after five cycles of adsorption-desorption. It was found that the ion-exchange and complexation interactions between the functional groups (-COO(-) and -NH2) of the hydrogels and Cu(2+) ion are the predominant adsorption mechanisms.
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http://dx.doi.org/10.1016/j.colsurfb.2013.01.030DOI Listing
June 2013

Preparation and characterization of magnetic alginate-chitosan hydrogel beads loaded matrine.

Drug Dev Ind Pharm 2012 Jul 18;38(7):872-82. Epub 2011 Nov 18.

Lanzhou University Second Hospital, Lanzhou, China.

The aim of this study was to use alginate-chitosan (Alg-CS) hydrogel beads for developing an oral water-soluble drug delivery system, occupying pH-sensitive property and superparamagnetic. Matrine as a model drug was loaded in Alg-CS hydrogel beads to study the release character of the delivery system. The amount of matrine released from the beads was relatively low in pH 2.5 over 8 h (34.90%), but nearly all of the initial drug content was released in simulated intestinal fluid (SIF, pH 6.8) within 8 h. The results demonstrated that Alg-CS hydrogel beads possess unique pH-dependent swelling behaviors. In addition, the magnetic beads were characterized by Fourier transform infrared spectroscopy, scanning electron microscope, X-ray diffractometry and vibrating-sample magnetometry. Magnetometer measurements data suggested that Alg-CS beads also had superparamagnetic property as well as fast magnetic response. It can be expected that the beads can deliver and release encapsulated anticancer agent at the tumor by the weak magnetic field, and hence could be potential candidates as an orally administered drug delivery system.
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http://dx.doi.org/10.3109/03639045.2011.630397DOI Listing
July 2012

Effect of surfactant on porosity and swelling behaviors of guar gum-g-poly(sodium acrylate-co-styrene)/attapulgite superabsorbent hydrogels.

Colloids Surf B Biointerfaces 2011 Nov 12;88(1):279-86. Epub 2011 Jul 12.

Key Laboratory of Chemistry of Northwestern Plant Resources, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, PR China.

Novel fast-swelling porous guar gum-g-poly(sodium acrylate-co-styrene)/attapulgite (GG-g-P(NaA-co-St)/APT) superabsorbent hydrogels were prepared by simultaneous free-radical graft copolymerization reaction of guar gum (GG), partially neutralized AA (NaA), styrene (St) and attapulgite (APT) using N,N'-methylenebisacrylamide (MBA) as a crosslinker and ammonium persulfate (APS) as an initiator in aqueous solution and the surfactant self-assembling templating pore-forming technique. Fourier transform infrared (FTIR) spectroscopy confirmed that the surfactant could be removed from the final hydrogel product by methanol/water (8:1, v/v) washing process and the surfactant only act as micelle template to form pores. The effect of surfactant type on the porous microstructure of the hydrogel was assessed by field emission scanning electron microscope (FESEM). It was shown that incorporation of proper amount of anionic surfactant sodium n-dodecyl sulfate (SDS) in the gelling process of the hydrogel can obviously enhance the swelling capacity and initial swelling rate. The salt-sensitivity of the SDS-added hydrogel in distilled water and 15 mmol/L NaCl, CaCl(2) solution or 15 mmol/L NaCl and CaCl(2) solution was investigated, and it was found that the swelling-deswelling capability is quite reversible. A similar reproducible on-off switching behavior was observed in the 1 mmol/L solution of phosphate buffer at pH 2.1 and 7.4.
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http://dx.doi.org/10.1016/j.colsurfb.2011.07.002DOI Listing
November 2011

Structurally designed synthesis of mechanically stable poly(benzoxazine-co-resol)-based porous carbon monoliths and their application as high-performance CO2 capture sorbents.

J Am Chem Soc 2011 Jul 6;133(29):11378-88. Epub 2011 Jul 6.

State Key Laboratory of Fine Chemicals, School of Chemical Engineering, Dalian University of Technology, Dalian 116024, P. R. China.

Porous carbon monoliths with defined multilength scale pore structures, a nitrogen-containing framework, and high mechanical strength were synthesized through a self-assembly of poly(benzoxazine-co-resol) and a carbonization process. Importantly, this synthesis can be easily scaled up to prepare carbon monoliths with identical pore structures. By controlling the reaction conditions, porous carbon monoliths exhibit fully interconnected macroporosity and mesoporosity with cubic Im3m symmetry and can withstand a press pressure of up to 15.6 MPa. The use of amines in the synthesis results in a nitrogen-containing framework of the carbon monolith, as evidenced by the cross-polarization magic-angle-spinning NMR characterization. With such designed structures, the carbon monoliths show outstanding CO(2) capture and separation capacities, high selectivity, and facile regeneration at room temperature. At ~1 bar, the equilibrium capacities of the monoliths are in the range of 3.3-4.9 mmol g(-1) at 0 °C and of 2.6-3.3 mmol g(-1) at 25 °C, while the dynamic capacities are in the range of 2.7-4.1 wt % at 25 °C using 14% (v/v) CO(2) in N(2). The carbon monoliths exhibit high selectivity for the capture of CO(2) over N(2) from a CO(2)/N(2) mixture, with a separation factor ranging from 13 to 28. Meanwhile, they undergo a facile CO(2) release in an argon stream at 25 °C, indicating a good regeneration capacity.
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http://dx.doi.org/10.1021/ja203857gDOI Listing
July 2011

Selective transformation of cellulose into sorbitol by using a bifunctional nickel phosphide catalyst.

ChemSusChem 2010 Jul;3(7):818-21

State Key Laboratory of Catalysis, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, P.O. Box 110, Dalian 116023, PR China.

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http://dx.doi.org/10.1002/cssc.201000092DOI Listing
July 2010

Adsorption-induced structural changes of gold cations from two- to three-dimensions.

Phys Chem Chem Phys 2010 Mar 4;12(12):3038-43. Epub 2010 Feb 4.

State Key Laboratory of Catalysis, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China.

Understanding the geometry structures of gold clusters, especially with adsorbates, is essential for designing highly active gold nanocatalysts. Here, we report a detailed theoretical study of the geometry structures of bare and CO-saturated Au(n)(+) (n = 4-6) clusters. It is found that the chemisorption of CO molecules leads to significant geometry changes of the gold clusters from two- to three-dimensions (3D), even for clusters as small as Au(4)(+). These gold cationic clusters exhibit characteristic coordination binding sites that have distinct electronic structures. We also find that commonly used density functional theory (DFT) methods have difficulty in accurately predicting energies of some isomers of Au(n)(+) clusters or Au(n)(CO)(n)(+) complexes, with the calculated relative energies strongly depending on the exchange-correlation functionals used. Caution must be exercised when using DFT methods as a blackbox for predicting the structures and energies of gold clusters.
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http://dx.doi.org/10.1039/b921367hDOI Listing
March 2010

A novel pH-sensitive magnetic alginate-chitosan beads for albendazole delivery.

Drug Dev Ind Pharm 2010 Jul;36(7):867-77

The Second Hospital of Lanzhou University, Lanzhou, China.

Background: Drug delivery system using polymer-coated magnetic carriers is considered as an effective strategy for passive targeting, which can not only increase drug utilization but also reduce the adverse reaction. With the carriers, sensitivity to physical stimuli (e.g., magnetic field, pH) has been developed and drugs were conjugated to form incorporating magnetic particles, so that drugs could be located to desire position.

Method: Novel magnetic alginate (Alg)-chitosan (CS) beads loaded with albendazole (ABZ) were prepared and evaluated for pH sensitivity and drug release characteristics. The effects of six different factors (Alg concentration, the weight ratio of drug to polymer, the weight ratio of magnetite nanoparticles to polymer, CaCl2 concentration, CS concentration, the volume ratio of Alg to CS) were studied on the swelling ability of the magnetic beads. The magnetic beads were characterized by Fourier transform infrared spectroscopy, scanning electron microscope, and vibrating sample magnetometry. In addition, the delivery behavior of ABZ from the magnetic beads was studied.

Result: The magnetic Alg-CS beads had showed unique pH-dependent swelling behaviors and a continuous release of ABZ. From the magnetometer measurements data, the beads also had superparamagnetic property as well as fast magnetic response.

Conclusion: The pH-sensitive magnetic beads may be used as a magnetic drug targeting system for ABZ in the gastrointestinal tract.
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http://dx.doi.org/10.3109/03639040903567117DOI Listing
July 2010

pH-sensitive magnetic alginate-chitosan beads for albendazole delivery.

Pharm Dev Technol 2011 Jun 5;16(3):228-36. Epub 2010 Feb 5.

The Second Hospital of Lanzhou University, Lanzhou, PR China.

Novel magnetic alginate-chitosan beads were prepared for the controlled delivery of albendazole. The magnetic beads were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), and vibrating sample magnetometry (VSM). The swelling behavior and in vitro release of albendazole from the beads has been investigated. The magnetic hydrogel beads had showed unique pH-dependent swelling behaviors and a continuous release of the entrapped albendazole occurred. From the magnetometer measurements data, the magnetic alginate-chitosan beads also had superparamagnetic property as well as fast magnetic response. The magnetic beads may be used as a magnetic drug targeting system for albendazole in the gastrointestinal tract.
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http://dx.doi.org/10.3109/10837451003592217DOI Listing
June 2011

[Immune response in BALB/c mice immunized with BCG expressing HBV truncated C gene and preS1 gene].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2010 Jan;26(1):28-30

Center Laboratory, Fourth Medical Military University, Xi'an 710032, China.

Aim: To study expressing of HBV truncated core and preS1 protein in BCG and to explore the effect of humoral and cellular immune response stimulated by the recombinant BCG.

Methods: A shuttle vector was constructed and was transformed into BCG which including truncated Core gene and preS1 gene of HBV. The recombinant BCGs were analyzed with SDS-PAGE and Western blot. The three groups of BALB/c mice were immunized with saline, BCG, BCG-pDE22 and BCG-pDE22-CS1 respectively. Then the antibody titer and CTL effects were evaluated.

Results: Compared with the control group, the recombinant BCG could express a new protein band of 24 kD which was consistent with the size of CS1 fused protein which displayed a good antigen-binding property by Western blot analysis. The antibody titer significantly increased and CTL effect apparently enhanced in the recombinant BCG immunized group.

Conclusion: BCG could be as live vector which carrying HBV related genes, which developing a novel vaccine against HBV.
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January 2010

Transition metal-tungsten bimetallic catalysts for the conversion of cellulose into ethylene glycol.

ChemSusChem 2010 ;3(1):63-6

State Key Laboratory of Catalysis, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, P.O. Box 110, Dalian 116023, PR China.

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http://dx.doi.org/10.1002/cssc.200900197DOI Listing
October 2010

[Adsorption of Pb2+ onto chitosan-grafted-poly (acrylic acid )/sepiolite composite].

Huan Jing Ke Xue 2009 Sep;30(9):2575-9

Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China.

A composite adsorbent chitosan-grafted-poly(acrylic acid)/sepiolite was prepared, and its pH-dependence for removing Pb2+, as well as adsorption isotherm and kinetics were estimated. The results indicate that poly (acrylic acid) has been grafted onto the backbone of chitosan, forming an organic-inorganic composite adsorbent. As-prepared adsorbent shows a coarse, porous and accidented surface, which can contribute to its adsorption kinetics. Under the conditions of pH 6.00, contact time of 30 min, initial Pb2+ concentration of 0.02 mol x L(-1) and amount of adsorbent of 0.10 g, the adsorption capacity of developed adsorbent for Pb2+ is found to be 638.9 mg x g(-1), about three times than that of sepiolite. When five cycles of adsorption-desorption process were carried out, the adsorption capacity decreased to 489.2 mg x g(-1), 76.6% to its original adsorption capacity. However, when sepiolite was used as the adsorbent, no sorption can be observed after three cycles of adsorption-desorption process. Compared to sepiolite, this composite adsorbent presents higher adsorption capacity, faster adsorption rate and better reusable ability.
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September 2009

Preparation and swelling properties of pH-sensitive sodium alginate/layered double hydroxides hybrid beads for controlled release of diclofenac sodium.

J Biomed Mater Res B Appl Biomater 2010 Jan;92(1):205-14

Center of Eco-material and Green Chemistry, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, People's Republic of China.

Layered double hydroxides (LDHs) are promising materials for the preparation of novel drug delivery matrices. To reveal the interaction between LDHs and polymers, a series of pH-sensitive sodium alginate/layered double hydroxides (SA/LDHs) hybrid beads were prepared using the simple surface crosslinking method. The SA/LDHs hybrid beads were characterized by FTIR, XRD, and SEM to study their structure and the role that LDHs play. Swelling behaviors in various aqueous solutions and drug loading as well as controlled release behavior of the SA/LDHs hybrid beads were also investigated by using diclofenac sodium (DS) as the model drug to reveal the effects of introduced LDHs. The results indicate that the positively charged LDHs layers are adsorbed on the negatively charged SA polymer chains through electrostatic interaction and act as inorganic crosslinkers in the three-dimensional network. In addition, a small part of DS molecules intercalate into layers of LDHs through an anion exchange process. The electrostatic interaction between LDHs and SA has restricted movability of the SA polymer chains, and then slows down swelling and dissolution rates of the SA/LDHs hybrid beads in aqueous solutions. The electrostatic interaction and the intercalation of DS into layers of LDHs have also improved the entrapment efficiency (EE) and controlled release behavior of SA/LDHs hybrid beads for DS.
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http://dx.doi.org/10.1002/jbm.b.31507DOI Listing
January 2010

[Radiological and histopathological changes of the periapical lesions induced by lipopolysaccharide in rats].

Shanghai Kou Qiang Yi Xue 2008 Aug;17(4):416-9

Department of Endodontics, College of Stomatology, Binzhou Medical College, Binzhou 256603, Shandong Province, China.

Purpose: To investigate histological and X-ray dynamic changes of periapical lesions induced by lipopolysaccharide(LPS) in rats.

Methods: Experimental periapical periodontitis models in 24 Wistar rats were established by exposing pulps, placing absorbent cotton with LPS, and exposing in the oral environment. Four experimental animals were sacrificed on the day 0,3,7,14,21, and 35, respectively. Root apical radiographs and tissue sections were taken in all the teeth combined with bone from sacrificed animals. Periapical histological changes and roentgenograms were observed. The results were analyzed statistically by F test and Student's t test with SPSS12.0 software package.

Results: On the day 3, infiltration of inflammatory cells in the periapical tissue was observed. Acute inflammatory states, severe inflammatory states and chronic inflammatory states were detected at 1 week, 2-week and 3-week,respectively. Cementum hyperplasia was observed at 5-week. Increasing periapical radiolucent areas were observed after 1 week. Intervention, there were significant differences in the periapical lesion areas at the different period time(P<0.01).

Conclusions: The procedures that induced periapical lesions in animal model established by exposing pulps of molar teeth,placing absorbent cotton with LPS, and exposing to the oral environment is similar to human natural progress of the same disease and has a shorter time duration. It is an ideal research model for periapical periodontitis.
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August 2008
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