Publications by authors named "Ai Guo"

98 Publications

Genome-wide association study reveals the genetic architecture of 27 agronomic traits in tomato.

Plant Physiol 2021 Aug;186(4):2078-2092

Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China.

Tomato (Solanum lycopersicum) is a highly valuable fruit crop, and yield is one of the most important agronomic traits. However, the genetic architecture underlying tomato yield-related traits has not been fully addressed. Based on ∼4.4 million single nucleotide polymorphisms obtained from 605 diverse accessions, we performed a comprehensive genome-wide association study for 27 agronomic traits in tomato. A total of 239 significant associations corresponding to 129 loci, harboring many previously reported and additional genes related to vegetative and reproductive development, were identified, and these loci explained an average of ∼8.8% of the phenotypic variance. A total of 51 loci associated with 25 traits have been under selection during tomato domestication and improvement. Furthermore, a candidate gene, Sl-ACTIVATED MALATE TRANSPORTER15, that encodes an aluminum-activated malate transporter was functionally characterized and shown to act as a pivotal regulator of leaf stomata formation, thereby affecting photosynthesis and drought resistance. This study provides valuable information for tomato genetic research and breeding.
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http://dx.doi.org/10.1093/plphys/kiab230DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8331143PMC
August 2021

Platelet-rich plasma attenuates interleukin-1β-induced apoptosis and inflammation in chondrocytes through targeting hypoxia-inducible factor-2α.

Tissue Cell 2021 Sep 8;73:101646. Epub 2021 Sep 8.

Department of Orthopedics, Beijing Friendship Hospital, Capital Medical University, 95 Yong an Road, Xicheng District, Beijing, 100050, China. Electronic address:

Osteoarthritis (OA) is a prevailing chronic disease in Orthopedics that characterized with severely damaged cartilage and subchondral bone, thus leading to profound disorders of synovial joints. Platelet-rich plasma (PRP) has been applied as a popular non-operative treatment option for promoting musculoskeletal healing. Our previous work demonstrated that PRP protected chondrocytes from interleukin-1β (IL-1β)-induced apoptosis in vitro. However, the underlying mechanism behind the treatment remains unclear. The current study aimed to unveil the molecular signaling underlying its protective role in chondrocytes. Rat chondrocytes were isolated from newborn Sprague Dawley rats and treated with 5 ng/mL IL-1β for 24 h. The expression of hypoxia-inducible factor 2α (HIF-2α) was determined in both mRNA and protein levels. Next, loss- and gain-of-function assays for HIF-2α were performed using small-interfering RNA (siRNA) specific for HIF-2α and adenovirus-mediated HIF-2α overexpression, respectively. In addition, cell apoptosis markers, matrix metalloproteinase (MMP)-1, 3, -9 and -13, and extracellular matrix-related genes were evaluated. Our results demonstrated that IL-1β induced distinct inflammation in chondrocytes. In addition, HIF-2α upregulated in the IL-1β-treated chondrocytes compared to the untreated cells. Interestingly, 10% PRP protected chondrocytes against IL-1β-induced apoptosis and matrix degradation, and meanwhile suppressed the HIF-2α activation. Furthermore, HIF-2α siRNA and HIF-2α overexpression experiments indicated that PRP induced chondroprotection through targeting HIF-2α. Taken together, our findings indicated that PRP attenuates IL-1β-induced chondrocyte apoptosis and inflammation at least partially through inhibiting HIF-2α.
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http://dx.doi.org/10.1016/j.tice.2021.101646DOI Listing
September 2021

Hair (H) interacts with SlZFP8-like to regulate the initiation and elongation of trichomes by modulating SlZFP6 expression in tomato.

J Exp Bot 2021 Sep 9. Epub 2021 Sep 9.

Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, Wuhan, China.

Trichomes are specialized glandular or non-glandular structures that provide physical or chemical protection against insect and pathogens attack. Trichomes in Arabidopsis, as typical non-glandular structures, have been extensively studied. However, the molecular mechanism underlying glandular trichome formation and elongation still remains largely unknown. We previously demonstrated that Hair (H) is essential for the formation of type I and type VI trichomes. Here, we found that overexpression of H increased the density and length of tomato trichomes. We revealed that H physically interacts with its close homolog SlZFP8-like (SlZFP8L) and SlZFP8L also directly interacts with Woolly (Wo) by biochemical assays. SlZFP8L overexpression plants showed increased trichome density and length. We further found that the expression of SlZFP6, encoding a C2H2 zinc finger protein, is positively regulated by H. We identified that SlZFP6, is a direct target of H through ChIP-qPCR, Y1H, and LUC assays. Similar to H and SlZFP8L, the overexpression of SlZFP6 also increased the density and length of tomato trichomes. Taken together, our results suggest that H interacts with SlZFP8-like to regulate the initiation and elongation of trichomes by modulating SlZFP6 expression in tomato.
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http://dx.doi.org/10.1093/jxb/erab417DOI Listing
September 2021

Association of patellofemoral morphology and alignment with the radiographic severity of patellofemoral osteoarthritis.

J Orthop Surg Res 2021 Sep 4;16(1):548. Epub 2021 Sep 4.

Department of Orthopedic, Beijing Friendship Hospital, Capital Medical University, No. 95 Yong'an Road, Xicheng, Beijing, 100050, People's Republic of China.

Background: Risk factors for the severity of patellofemoral osteoarthritis (PFOA) are poorly understood. This research aims to evaluate the association between patellofemoral joint (PFJ) morphology and alignment with the radiographic severity of PFOA.

Methods: A retrospective analysis of CT scan and lateral radiograph data were acquired in patients with PFOA. The radiographic grade of PFOA and tibiofemoral osteoarthritis (TFOA), lateral and medial trochlear inclination angle, sulcus angle, and the Wiberg classification of patella morphology, the congruence angle, patellar tilt angle, and lateral patellar angles, and tibial tubercle trochlear groove distance (TT-TG) and patella height (i.e., Caton-Deschamps index) were assessed using CT scans and sagittal radiographs of the knee. All the PFJ morphology and alignment data were divided into quarters, and the relationships between each of these measures and the severity of PFOA were investigated.

Results: By studying 150 patients with PFOA, we found a U-shaped relationship between the Caton-Deschamps index and the severity of PFOA (P < 0.001). A lower value of sulcus angle and lateral patellar angle, a higher value of congruence angle, and type III patella were associated with more severity of lateral PFOA. Compared with the highest quarter of each measure, the adjusted odds ratios (OR) of the severity of PFOA in the lowest quarter of sulcus angle, lateral patellar angle, and congruence angle; and type I patella was 8.80 (p = 0.043), 16.51 (P < 0.001), 0.04 (P < 0.001), and 0.18 (p = 0.048) respectively.

Conclusions: Extreme value of patella height, a higher value of lateral patellar displacement and lateral patellar tilt, lower value of sulcus angle, and type III patella were associated with more severity of PFOA.
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http://dx.doi.org/10.1186/s13018-021-02681-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8418017PMC
September 2021

Associations of immunological features with COVID-19 severity: a systematic review and meta-analysis.

BMC Infect Dis 2021 Aug 3;21(1):738. Epub 2021 Aug 3.

Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.

Background: COVID-19 has spread widely worldwide, causing millions of deaths. We aim to explore the association of immunological features with COVID-19 severity.

Methods: We conducted a meta-analysis to estimate mean difference (MD) of immune cells and cytokines levels with COVID-19 severity in PubMed, Web of Science, Scopus, the Cochrane Library and the grey literature.

Results: A total of 21 studies with 2033 COVID-19 patients were included. Compared with mild cases, severe cases showed significantly lower levels of immune cells including CD3 T cell (× 10, MD, - 413.87; 95%CI, - 611.39 to - 216.34), CD4 T cell (× 10, MD, - 203.56; 95%CI, - 277.94 to - 129.18), CD8 T cell (× 10, MD, - 128.88; 95%CI, - 163.97 to - 93.79), B cell (× 10/L; MD, - 23.87; 95%CI, - 43.97 to - 3.78) and NK cell (× 10/L; MD, - 57.12; 95%CI, - 81.18 to - 33.06), and significantly higher levels of cytokines including TNF-α (pg/ml; MD, 0.34; 95%CI, 0.09 to 0.59), IL-5 (pg/ml; MD, 14.2; 95%CI, 3.99 to 24.4), IL-6 (pg/ml; MD, 13.07; 95%CI, 9.80 to 16.35), and IL-10 (pg/ml; MD, 2.04; 95%CI, 1.32 to 2.75), and significantly higher levels of chemokines as MCP-1 (SMD, 3.41; 95%CI, 2.42 to 4.40), IP-10 (SMD, 2.82; 95%CI, 1.20 to 4.45) and eotaxin (SMD, 1.55; 95%CI, 0.05 to 3.05). However, no significant difference was found in other indicators such as Treg cell (× 10, MD, - 0.13; 95%CI, - 1.40 to 1.14), CD4/CD8 ratio (MD, 0.26; 95%CI, - 0.02 to 0.55), IFN-γ (pg/ml; MD, 0.26; 95%CI, - 0.05 to 0.56), IL-2 (pg/ml; MD, 0.05; 95%CI, - 0.49 to 0.60), IL-4 (pg/ml; MD, - 0.03; 95%CI, - 0.68 to 0.62), GM-CSF (SMD, 0.44; 95%CI, - 0.46 to 1.35), and RANTES (SMD, 0.94; 95%CI, - 2.88 to 4.75).

Conclusion: Our meta-analysis revealed significantly lower levels of immune cells (CD3 T, CD4 T, CD8 T, B and NK cells), higher levels of cytokines (TNF-α, IL-5, IL-6 and IL-10) and higher levels of chemokines (MCP-1, IP-10 and eotaxin) in severe cases in comparison to mild cases of COVID-19. Measurement of immunological features could help assess disease severity for effective triage of COVID-19 patients.
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http://dx.doi.org/10.1186/s12879-021-06457-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8329624PMC
August 2021

Gallium (Ga)-strontium (Sr) layered double hydroxide composite coating on titanium substrates for enhanced osteogenic and antibacterial abilities.

J Biomed Mater Res A 2021 Jul 29. Epub 2021 Jul 29.

Laboratory Research Center, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Bacterial infection and poor osteogenic capacity can result in the loosing or failure of titanium (Ti)-based implants in the clinic. Therefore, it is urgent to design an effective approach to enhance the osteogenic property and restrict bacterial activity. In this study, a layered double hydroxide (LDH) composed of Ga and Sr ions on Ti substrates by a hydrothermal method, then calcined in 250°C and denoted as LDH250. The scanning electron microscopy (SEM), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS) were confirmed that the LDH films were successfully formed on the Ti substrates. Importantly, the obtained LDH films can induce an alkaline microenvironment around the Ti surface and regulate the behaviors of osteogenic cells and bacteria. In vitro cellular experiments, the LDH250 can enhance the differentiation of both MC3T3-E1 cells and osteoblasts, stimulate alkaline phosphatase activity (ALP), collagen secretion, and mineralization levels. Meanwhile, antimicrobial assay against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) demonstrated that the LDH250 samples had strong antibacterial abilities, which attributed to the release profile of Ga could act as a "Trojan horse" to destroy the bacterial iron metabolism, inducing of local alkaline environment, and producing reactive oxygen species. Hence, this study provides an effective method for reducing antibacterial infection and enhancing the bone integrative capacity of Ti-based implants for orthopedic applications.
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http://dx.doi.org/10.1002/jbm.a.37284DOI Listing
July 2021

L2, a chloroplast metalloproteinase, regulates fruit ripening by participating in ethylene autocatalysis under the control of ERFs.

J Exp Bot 2021 Jul 13. Epub 2021 Jul 13.

Although autocatalytic ethylene biosynthesis plays an important role in the ripening of climacteric fruits, our knowledge of the network that promotes autocatalytic ethylene biosynthesis remains limited. We identified white fruit (wf), a tomato mutant that produces immature fruit that are white and that ripen slowly. We found that an inversion on chromosome 10 that disrupts the LUTESCENT2 gene, and the white fruit is allelic to lutescent 2. Using CRISPR-Cas9 technology we knocked out L2 in wild type tomato and found that the l2-cr mutants produced phenotype that were very similar to white fruit (lutescent 2). In the l2-cr fruit, chloroplast development was impaired and the accumulation of carotenoids and lycopene occurred more slowly than in wild type. During fruit ripening in l2-cr mutants, the peak of ethylene release was delayed, less ethylene was produced and the expression of ACO genes was significantly suppressed. We also found that exogenous ethylene induces the expression of L2 and that ERF.B3, an ethylene response factor, binds the promoter of the L2 gene and activates its transcription. Thus, the expression of L2 is regulated by exogenous ethylene. Taken together, our results indicate that ethylene may affect the expression of the L2 gene and that the L2 gene participates in autocatalytic ethylene biosynthesis during tomato fruit ripening.
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http://dx.doi.org/10.1093/jxb/erab325DOI Listing
July 2021

Association between changes in hip-knee-ankle angle and hindfoot alignment after total knee arthroplasty for varus knee osteoarthritis.

BMC Musculoskelet Disord 2021 Jul 6;22(1):610. Epub 2021 Jul 6.

Department of Orthopaedics, Beijing Friendship Hospital, Capital Medical University, No. 95, Yongan Road, Xicheng District, 100050, Beijing, China.

Background: The change in hip-knee-ankle (HKA) angle after total knee arthroplasty (TKA) may cause an adjustment in hindfoot alignment (HFA). However, the relationship between the changes in HKA angle and HFA is still not well studied. This study aimed to investigate the association between HKA angle and hindfoot alignment changes after TKA for varus knee osteoarthritis.

Methods: A prospective study was carried out in which 108 patients with varus knee deformities were radiographically and clinically evaluated before and 3 months after TKA. The relationship of change in HFA with correction in HKA angle was investigated.

Results: The results showed that the HFA was adjusted significantly by 3 months after TKA (p < 0.001), along with improved American Orthopaedic Foot and Ankle Society (AOFAS) ankle hindfoot score (p < 0.001). Next, a univariate correlation and linear regression analysis showed that the change in HFA was weakly correlated with the change in HKA angle (r=-0.262, β=-0.14, 95 % CI: -0.23 to -0.04, P = 0.006). Further stratified analysis and interaction tests revealed that age has a distinct effect on the correlation between the changes in HFA and HKA angle. The correlation was dramatically greater in the group under 65 years (r=-0.474, β=-0.26, 95 % CI: -0.41 to -0.12, P = 0.001), whilst, no correlation was observed in those above 65 years old (r=-0.036, β=-0.02, 95 % CI: -0.14 to 0.11, P = 0.779).

Conclusions: Our findings indicated that correction of HKA after TKA tend to promote adjustment in the hindfoot alignment toward re-balance of the whole lower limb weight-bearing axis. However, this mechanism obviously weakens in elderly patients. Therefore, if apparent hindfoot deformity exists in these patients before TKA, more perioperative intervention is required for hindfoot adjustment, and even HKA undercorrection may be considered.
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http://dx.doi.org/10.1186/s12891-021-04488-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261933PMC
July 2021

Corrigendum to "Type 2 diabetes-induced overactivation of P300 contributes to skeletal muscle atrophy by inhibiting autophagic flux" [Life Sci. 2020 Oct 1;258:118243].

Life Sci 2021 Aug 11;279:119589. Epub 2021 May 11.

Department of Geriatrics, The First Affiliated Hospital of Chongqing Medical University, Youyi Road 1, Chongqing 400042, China. Electronic address:

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http://dx.doi.org/10.1016/j.lfs.2021.119589DOI Listing
August 2021

FGF19 protects skeletal muscle against obesity-induced muscle atrophy, metabolic derangement and abnormal irisin levels via the AMPK/SIRT-1/PGC-α pathway.

J Cell Mol Med 2021 04 10;25(7):3585-3600. Epub 2021 Mar 10.

Department of Geriatrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Obesity is associated with biological dysfunction in skeletal muscle. As a condition of obesity accompanied by muscle mass loss and physical dysfunction, sarcopenic obesity (SO) has become a novel public health problem. Human fibroblast growth factor 19 (FGF19) plays a therapeutic role in metabolic diseases. However, the protective effects of FGF19 on skeletal muscle in obesity and SO are still not completely understood. Our results showed that FGF19 administration improved muscle loss and grip strength in young and aged mice fed a high-fat diet (HFD). Increases in muscle atrophy markers (FOXO-3, Atrogin-1, MuRF-1) were abrogated by FGF19 in palmitic acid (PA)-treated C2C12 myotubes and in the skeletal muscle of HFD-fed mice. FGF19 not only reduced HFD-induced body weight gain, excessive lipid accumulation and hyperlipidaemia but also promoted energy expenditure (PGC-1α, UCP-1, PPAR-γ) in brown adipose tissue (BAT). FGF19 treatment restored PA- and HFD-induced hyperglycaemia, impaired glucose tolerance and insulin resistance (IRS-1, GLUT-4) and mitigated the PA- and HFD-induced decrease in FNDC-5/irisin expression. However, these beneficial effects of FGF19 on skeletal muscle were abolished by inhibiting AMPK, SIRT-1 and PGC-1α expression. Taken together, this study suggests that FGF19 protects skeletal muscle against obesity-induced muscle atrophy, metabolic derangement and abnormal irisin secretion partially through the AMPK/SIRT-1/PGC-α signalling pathway, which might be a potential therapeutic target for obesity and SO.
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http://dx.doi.org/10.1111/jcmm.16448DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034456PMC
April 2021

A novel biocomposite scaffold with antibacterial potential and the ability to promote bone repair.

J Biomater Appl 2021 Sep 17;36(3):474-480. Epub 2021 Feb 17.

Department of Orthopaedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, Yuzhong, People's Republic of China.

Clinical treatment of bone defects caused by trauma, tumor resection and other bone diseases, especially bone defects that can lead to infection, remains a major challenge. Currently, autologous bone implantation is the gold standard for treatment of bone defects, but it is limited by secondary trauma and insufficient autologous material. Moreover, postoperative infection is an important factor affecting bone healing.AcN-RADARADARADARADA-CONH2 (RADA) is a new type of self-assembling peptide(SAP) composed of Arg,Ala,Asp and other amino acids was designed and prepared. The "RADA" self-assembling peptide hydrogels has excellent biological activity and it's completely biodegradable and non-toxic.It is also have been confirmed to promote cell proliferation, wound healing, tissue repair, and drug delivery. To promote bone regeneration and simultaneously prevent bacterial infection, we designed biocomposite scaffolds comprising RADA and calcium phosphate cement (CPC), termed RADA-CPC. The morphological features of the scaffold were characterized by scanning electron microscopy (SEM). In vitro studies demonstrated that RADA-CPC enhances osteoblast proliferation, differentiation and mineralization. In addition, the scaffold was used as a drug delivery system to treat postoperative infections by sustained release of ciprofloxacin (CIP). The RADA-CPC scaffold may have potential application prospects in orthopedics field because of its role in promoting bone repair and as a sustained-release drug carrier to prevent infections.
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http://dx.doi.org/10.1177/0885328221994448DOI Listing
September 2021

TGF-β1-containing exosomes derived from bone marrow mesenchymal stem cells promote proliferation, migration and fibrotic activity in rotator cuff tenocytes.

Regen Ther 2020 Dec 21;15:70-76. Epub 2020 Jul 21.

Department of Orthopedics, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, PR China.

Objective: This study aimed to investigate effects of TGF-β1-containing exosomes derived from bone marrow mesenchymal stem cells (BMSC) on cell function of rotator cuff tenocytes and its implication to rotator cuff tear.

Methods: The primary BMSC and rotator cuff tenocytes were extracted and cultured. Identification of BMSC were performed by observing cell morphology and measurement of surface biomarkers by flow cytometry. BMSC-derived exosomes were extracted and identified by using electron microscopy, nanoparticle-tracking analysis (NTA) and western blotting. Cell proliferation and cell cycle were measured by CCK-8 assay and flow cytometry assay, respectively. Transwell assay was used for detection of tenocytes migration. The fibrotic activity of tenocytes was determined via qPCR and western blotting assays.

Results: BMSC and BMSC-derived exosomes were successfully extracted. Treatment of BMSC-derived exosomes or TGF-β1 promoted cell proliferation, migration and increased cell ratio of (S + G2/M) phases in tenocytes, as well as enhanced the expression levels of fibrotic activity associated proteins. However, inhibition of TGF-β1 by transfection of sh-TGF-β1 or treatment of TGFβR I/II inhibitor partially reversed the impact of BMSC-derived exosomes on tenocytes function.

Conclusion: Taken together, TGF-β1-containing exosomes derived from BMSC promoted proliferation, migration and fibrotic activity in rotator cuff tenocytes, providing a new direction for treatment of rotator cuff tendon healing.
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http://dx.doi.org/10.1016/j.reth.2020.07.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7770343PMC
December 2020

The Current State of Doctors' Communication Skills in Mainland China from the Perspective of Doctors' Self-evaluation and Patients' Evaluation: A Cross-Sectional Study.

Authors:
Ai Guo Pei Wang

Patient Educ Couns 2021 07 23;104(7):1674-1680. Epub 2020 Dec 23.

School of Psychiatry, Wenzhou Medical University, Wenzhou, China; East China Normal University, Faculty of Education, Shanghai, China. Electronic address:

Objective: To assess doctors' communication skills in mainland China using the SEGUE Framework.

Methods: A survey on doctors' communication skills with doctors (n = 1361) and patients (n = 1757) from 14 provinces in eastern, central and western China was conducted.

Results: The doctors' self-evaluation scores were higher than patients' evaluations (p < .001). The scores of female doctors were higher than males (p = .022). Both doctors' self-evaluations and patients' evaluations indicated that the scores of doctors in tertiary hospitals were higher than those in primary hospitals.

Conclusion: Doctors' communication skills don't match patients' needs. Female doctors are more empathetic and patient than male doctors and consequently have better communication skills. Doctors in tertiary hospitals have better communication skills because tertiary hospitals provide more training opportunities in communication skills and have better medical services and management.

Practice Implications: This study confirms the applicability of the SEGUE Framework to doctors and patients in mainland China. The effectiveness of cultivating doctors' communication skills should be evaluated through feedback from the perspective of both doctors and patients. Medical institutes need to prioritize patients' needs and provide training in doctors' communication skills to address the discrepancy in the perceptions of doctors and patients.
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http://dx.doi.org/10.1016/j.pec.2020.12.013DOI Listing
July 2021

Genome-Wide Identification and Molecular Characterization of the Growth-Regulating Factors-Interacting Factor Gene Family in Tomato.

Genes (Basel) 2020 11 28;11(12). Epub 2020 Nov 28.

Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, 430070 Wuhan, China.

Growth-regulating factors-interacting factor (GIF) proteins play crucial roles in the regulation of plant growth and development. However, the molecular mechanism of GIF proteins in tomato is poorly understood. Here, four genes (named , , , and ) were identified from the tomato genome and clustered into two major clades by phylogenetic analysis. The gene structure and motif pattern analyses showed similar exon/intron patterns and motif organizations in all the . We identified 33 -acting regulatory elements (CAREs) in the promoter regions of the . The expression profiling revealed the four are expressed in various tissues and stages of fruit development and induced by phytohormones (IAA and GA). The subcellular localization assays showed all four GIFs were located in nucleus. The yeast two-hybrid assay indicated various growth-regulating factors (SlGRFs) proteins interacted with the four SlGIF proteins. However, SlGRF4 was a common interactor with the SlGIF proteins. Moreover, a higher co-expression relationship was shown between three genes and five genes. The protein association network analysis found a chromodomain helicase DNA-binding protein (CHD) and an actin-like protein to be associated with the four SlGIF proteins. Overall, these results will improve our understanding of the potential functions of genes and act as a base for further functional studies on in tomato growth and development.
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http://dx.doi.org/10.3390/genes11121435DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760089PMC
November 2020

Absolute Eosinophil Count Predicts Intensive Care Unit Transfer Among Elderly COVID-19 Patients From General Isolation Wards.

Front Med (Lausanne) 2020 4;7:585222. Epub 2020 Nov 4.

Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

As of June 1, 2020, coronavirus disease 2019 (COVID-19) has caused a global pandemic and resulted in over 370,000 deaths worldwide. Early identification of COVID-19 patients who need to be admitted to the intensive care unit (ICU) helps to improve the outcomes. We aim to investigate whether absolute eosinophil count (AEC) can predict ICU transfer among elderly COVID-19 patients from general isolation wards. A retrospective study of 94 elderly patients older than 60 years old with COVID-19 was conducted. We compared the basic clinical characteristics and levels of inflammation markers on admission to general isolation wards and the needs for ICU transfer between the eosinopenia (AEC on admission <20 cells/μl) and non-eosinopenia (AEC ≥20 cells/μl) groups. There was a significantly higher ICU transfer rate in the eosinopenia group than in the non-eosinopenia group (51 vs. 9%, < 0.001). Multivariate analysis revealed that eosinopenia was associated with an increased risk of ICU transfer in elderly COVID-19 patients [adjusted odds ratio (OR) 6.12 (95% CI, 1.23-30.33), = 0.027] after adjustment of age, lymphocyte count, neutrophil count, C-reactive protein (CRP), and ferritin levels. The eosinopenia group had higher levels of CRP, ferritin, and cytokines [interleukin-2 receptor (IL-2R), interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-10 (IL-10), and tumor necrosis factor-α (TNF-α)] than the non-eosinophil group ( < 0.001). The area under the curve of AEC on admission for predicting ICU transfer among elderly COVID-19 patients was 0.828 (95% CI, 0.732-0.923). The best cut-off value of AEC was 25 cells/μl with a sensitivity of 91% and a specificity of 71%, respectively. Absolute eosinophil count on admission is a valid predictive marker for ICU transfer among elderly COVID-19 patients from general isolation wards and, therefore, can help case triage and optimize ICU utilization, especially for health care facilities with limited ICU capacity.
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http://dx.doi.org/10.3389/fmed.2020.585222DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7673383PMC
November 2020

NF-Y plays essential roles in flavonoid biosynthesis by modulating histone modifications in tomato.

New Phytol 2021 03 21;229(6):3237-3252. Epub 2020 Dec 21.

The Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, China.

NF-Y transcription factors are reported to play diverse roles in a wide range of biological processes in plants. However, only a few active NF-Y complexes are known in plants and the precise functions of NF-Y complexes in flavonoid biosynthesis have not been determined. Using various molecular, genetic and biochemical approaches, we found that NF-YB8a, NF-YB8b and NF-YB8c - a NF-YB subgroup - can interact with a specific subgroup of NF-YC and then recruit either of two distinct NF-YAs to form NF-Y complexes that bind the CCAAT element in the CHS1 promoter. Furthermore, suppressing the expression of particular NF-YB genes increased the levels of H3K27me3 at the CHS1 locus and significantly suppressed the expression of CHS1 during tomato fruit ripening, which led to the development of pink-coloured fruit with colourless peels. Altogether, by demonstrating that NF-Y transcription factors play essential roles in flavonoid biosynthesis and by providing significant molecular insight into the regulatory mechanisms that drive the development of pink-coloured tomato fruit, we provide a major advance to our fundamental knowledge and information that has considerable practical value for horticulture.
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http://dx.doi.org/10.1111/nph.17112DOI Listing
March 2021

Perioperative analgesia after intrathecal morphine or local infiltration anesthesia for total knee replacement: A protocol for randomized controlled trial.

Medicine (Baltimore) 2020 Sep;99(39):e22394

Department of Orthopedics, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

Objective: We perform this protocol for randomized controlled trial to compare the efficacy of intrathecal morphine and local infiltration anesthesia (LIA) in the treatment of the postoperative pain after total knee replacement (TKR).

Methods: This is a randomized controlled, single center trial which was performed from March 2019 to March 2020. This trial is conducted according to the SPIRIT Checklist of randomized researches. It is authorized via the Ethics Committee of Beijing Friendship Hospital (2019-P2-050-01). Eighty participants who undergo TKR were randomized into 2 groups. Intrathecal morphine group: 0.1 mg of the morphine was intrathecally injected, and the spinal anesthetic was injected at the same time in the group LIA; In the LIA group: the knee joint was infiltrated with epinephrine, ketorologic acid and ropivacaine in the process of operation, and the identical mixture was injected 2 bolus through the intraarticular catheter after operation. The main outcome variables were the visual analog scale and the consumption amount of opioid every 6-hour interval within 2 days postoperatively. The secondary outcome variables were the side effects associated with opioid, the length of hospital stay, motion range, and the loss of blood collected by the closed suction drainage. All the required analyses were carried out via applying the SPSS for Windows Version 19.0.

Results: The clinical outcome variables between groups were shown in .

Conclusion: This protocol will provide the evidence on which technique can achieve better analgesia after TKR.
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http://dx.doi.org/10.1097/MD.0000000000022394DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7523756PMC
September 2020

Brain transforms natural killer cells that exacerbate brain edema after intracerebral hemorrhage.

J Exp Med 2020 12;217(12)

Department of Neurology, Tianjin Neurological Institute, Tianjin Medical University General Hospital, Tianjin, China.

Perihematomal edema (PHE) occurs within hours after intracerebral hemorrhage (ICH), leading to secondary injury manifested by impaired blood-brain barrier (BBB) integrity and destruction of adjacent tissue. To dissect the mechanisms underlying PHE formation, we profiled human and mouse perihematomal tissues and identified natural killer (NK) cells as the predominant immune cell subset that outnumbers other infiltrating immune cell types during early stages of ICH. Unbiased clustering of single-cell transcriptional profiles revealed two major NK cell subsets that respectively possess high cytotoxicity or robust chemokine production features in the brain after ICH, distinguishing them from NK cells of the periphery. NK cells exacerbate BBB disruption and brain edema after ICH via cytotoxicity toward cerebral endothelial cells and recruitment of neutrophils that augment focal inflammation. Thus, brain-bound NK cells acquire new features that contribute to PHE formation and neurological deterioration following ICH.
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http://dx.doi.org/10.1084/jem.20200213DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7526480PMC
December 2020

Novel Hoberman Sphere Design for Interlaced [email protected] Architecture with Atomic Layer Deposition-Coated TiO Overlayer as Advanced Anodes in Li-Ion Battery.

ACS Appl Mater Interfaces 2020 Sep 20;12(35):39282-39292. Epub 2020 Aug 20.

Tianjin International Joint Research Centre of Surface Technology for Energy Storage Materials, College of Physics and Materials Science, Tianjin Normal University, Tianjin 300387, China.

The Hoberman sphere is a stable and stretchable spatial structure with a unique design concept, which can be taken as the ideal prototype of the internal mechanical/conductive skeleton for the anode with large volume change. Herein, MnO nanoparticles are interlaced with a Hoberman sphere-like interconnected carbon nanotube (CNT) network via a facile self-assembly strategy in which MnO can "locally expand" in the CNT network, limit the volume expansion to the interior space, and maintain a stable outer surface of the hybrid particle. Furthermore, an ultrathin uniform ALD-coated TiO shell is adopted to stabilize the solid electrolyte interphase (SEI), provide high electron conductivity and lithium ion (Li) diffusivity with lithiated LiTiO, and enhance the reaction kinetics of the MnO by an "electron-density enhancement effect". With this design, the [email protected]/TiO exhibits a high capacity of 1064 mAh g at 0.1 A g, a stable cycling stability over 200 cycles, a superior rate capability, and a commercial-level areal capacity of 4.9 mAh cm. In this way, a novel electrode design strategy is achieved by the Hoberman sphere-like CNT design along with the in situ porous formation, which can not only achieve a high-performance anode for LIBs but also can be widely adapted in a variety of advanced electrode materials for alkali metal ion batteries.
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http://dx.doi.org/10.1021/acsami.0c11282DOI Listing
September 2020

Type 2 diabetes-induced overactivation of P300 contributes to skeletal muscle atrophy by inhibiting autophagic flux.

Life Sci 2020 Oct 10;258:118243. Epub 2020 Aug 10.

Department of Geriatrics, The First Affiliated Hospital of Chongqing Medical University, Youyi Road 1, Chongqing 400042, China. Electronic address:

Aims: Although autophagy impairment is a well-established cause of muscle atrophy and P300 has recently been identified as an important regulator of autophagy, the effects of P300 on autophagy and muscle atrophy in type 2 diabetes (T2D) remain unexplored. We aimed at characterizing the role of P300 in diabetic muscle and its underlying mechanism.

Main Methods: Protein levels of phosphorylated P300, total P300, acetylated histone H3, LC3, p62 and myosin heavy chain, and mRNA levels of Atrogin-1 and MuRF1 were analyzed in palmitic acid (PA)-treated myotubes and db/db mice. Autophagic flux was assessed using transmission electron microscopy, immunofluorescence and mRFP-GFP-LC3 lentivirus transfection in cells. Muscle weight, blood glucose and grip strength were measured in mice. Hematoxylin and eosin (H&E) staining was performed to determine changes in muscle fiber size. To investigate the effects of P300 on autophagy and myofiber remodeling, a P300 specific inhibitor, c646, was utilized. 3-Methyladenine (3-MA) was utilized to inhibit autophagosomes formation, and chloroquine (CQ) was used to block autophagic flux.

Key Findings: Phosphorylation of P300 in response to PA enhanced its activity and subsequently suppressed autophagic flux, leading to atrophy-related morphological and molecular changes in myotubes. Inhibition of P300 reestablished autophagic flux and ameliorated PA-induced myotubes atrophy. However, this effect was largely abolished by co-treatment with the autophagy inhibitor CQ. In vivo results demonstrated that inhibition of P300 partially rescued muscle wasting in db/db mice, accompanied with autophagy reactivation.

Significance: The findings revealed that T2D-induced overactivation of P300 contributes to muscle atrophy by blocking autophagic flux.
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http://dx.doi.org/10.1016/j.lfs.2020.118243DOI Listing
October 2020

Comparison of OSTA, FRAX and BMI for Predicting Postmenopausal Osteoporosis in a Han Population in Beijing: A Cross Sectional Study.

Clin Interv Aging 2020 17;15:1171-1180. Epub 2020 Jul 17.

Department of Orthopedics, Beijing Friendship Hospital, Capital Medical University, Beijing, People's Republic of China.

Purpose: To validate the efficacies of three screening tools including the Osteoporosis Self-Assessment Tool for Asians (OSTA), Fracture Risk Assessment Tool (FRAX) without bone mineral density (BMD), and body mass index (BMI) for predicting postmenopausal osteoporosis (OP) and to define the ideal thresholds for avoidance of dual-energy X-ray absorptiometry (DXA) scanning in a Han Chinese population in Beijing.

Patients And Methods: A total of 2055 community-dwelling Han Beijing postmenopausal females aged ≥45 years were enrolled in this study. All participants completed a questionnaire, and BMD was measured by DXA. OP was defined by a T-score at least -2.5 SD less than that of average young adults in different diagnostic criteria [lumbar spine, femoral neck, total hip, worst hip, WHO]. The abilities of the OSTA, FRAX, and BMI to predict OP were analyzed by receiver operating characteristic (ROC) curves. Sensitivity, specificity, and area under the ROC curves (AUC) were calculated. Ideal thresholds for identifying OP were proposed.

Results: The prevalence of OP ranged from 8.1% to 28.4% according to different diagnostic criteria. The AUC range for the OSTA (0.758-0.849) was similar to the FRAX (0.728-0.855), which revealed that both tools predicted OP reliably. The AUC range for BMI was 0.643-0.682, suggesting limited predictive value. According to WHO criteria, the AUC values for the FRAX for hip fracture risk (FRAX-HF) and for the OSTA were 0.796 and 0.798, with corresponding sensitivities of 74.79% and 69.64% and specificities of 70.45% and 75.07%, respectively. At defined thresholds, the FRAX-HF and OSTA allowed avoidance of DXA in 42.4-37.6% of participants, at a cost of missing only 7.2-8.6% of individuals with OP.

Conclusion: The OSTA and FRAX-HF may be reliable and effective tools for identifying postmenopausal OP in the Han Beijing population without BMD.
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http://dx.doi.org/10.2147/CIA.S257166DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7381824PMC
December 2020

Sarcopenic obesity: Myokines as potential diagnostic biomarkers and therapeutic targets?

Exp Gerontol 2020 10 21;139:111022. Epub 2020 Jul 21.

Department of Geriatrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China. Electronic address:

Sarcopenic obesity (SO) is a condition characterized by the occurrence of both sarcopenia and obesity and imposes a heavy burden on the health of the elderly. Controversies and challenges regarding the definition, diagnosis and treatment of SO still remain because of its complex pathogenesis and limitations. Over the past few decades, numerous studies have revealed that myokines secreted from skeletal muscle play significant roles in the regulation of muscle mass and function as well as metabolic homeostasis. Abnormalities in myokines may trigger and promote the pathogenesis underlying age-related and metabolic diseases, including obesity, sarcopenia, type 2 diabetes (T2D), and SO. This review mainly focuses on the role of myokines as potential biomarkers for the early diagnosis and therapeutic targets in SO.
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http://dx.doi.org/10.1016/j.exger.2020.111022DOI Listing
October 2020

[The regulation of ubiquitination in milk fat synthesis in bovine].

Yi Chuan 2020 Jun;42(6):548-555

College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271000, China.

Ubiquitination signaling is the main pathway of protein degradation in eukaryotic cells. Ubiquitin-proteasome system degrades the ubiquitinated cytoplasmic proteins and lysosome pathway mainly degrades the ubiquitinated membrane proteins. Previous studies have shown that ubiquitination signaling plays a critical role in fatty acids synthesis. In the process of fatty acids import, disruption of ubiquitination could prevent the degradation of fatty acid transport proteins, thereby promoting fatty acids import and milk fat synthesis in bovine primary mammary epithelial cells. In this review, we summarize the signal transduction and regulation mechanism of ubiquitination signaling in milk fat synthesis, which may provide references and new ideas for future research on milk fat traits in dairy cows.
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http://dx.doi.org/10.16288/j.yczz.20-037DOI Listing
June 2020

Safety and effectiveness of a Tai Chi-based cardiac rehabilitation programme for chronic coronary syndrom patients: study protocol for a randomised controlled trial.

BMJ Open 2020 07 5;10(7):e036061. Epub 2020 Jul 5.

College of Physical Education and Sports, Beijing Normal University, Beijing, China

Introduction: Preliminary evidence from clinical observations suggests that Tai Chi exercise may offer potential benefits for patients with chronic coronary syndrom (CCS). However, the advantages for CCS patients to practice Tai Chi exercise as rehabilitation have not been rigorously tested and there is a lack of consensus on its benefits. This study aims to develop an innovative Tai Chi Cardiac Rehabilitation Program (TCCRP) for CCS patients and to assess the efficacy, safety and acceptability of the programme.

Methods And Analysis: We propose to conduct a multicentre randomised controlled clinical trial comprising of 150 participants with CCS. The patients will be randomly assigned in a 1:1 ratio into two groups. The intervention group will participate in a supervised TCCRP held three times a week for 3 months. The control group will receive supervised conventional exercise rehabilitation held three times a week for 3 months. The primary and secondary outcomes will be assessed at baseline, 1 month, 3 months after intervention and after an additional 3-month follow-up period. Primary outcome measures will include a score of 36-Item Short Form Survey and Chinese Perceived Stress Scale. The secondary outcome measures will include body composition, cardiopulmonary exercise test, respiratory muscle function, locomotor skills, echocardiogram, New York Heart Association classification, heart rate recovery time and laboratory examination. Other measures also include Seattle Angina Scale, Pittsburgh Sleep Quality Index, Patient Health Questionnaire-9, Generalized Anxiety Disorder-7 and Berg Balance Scale. All adverse events will be recorded and analysed.

Ethics And Dissemination: This study conforms to the principles of the Declaration of Helsinki and relevant ethical guidelines. Ethical approval has been obtained from the Ethics Committee of Chinese People's Libration Army General Hospital (approval number: S2019-060-02). Findings from this study will be published and presented at conferences for widespread dissemination of the results.

Trial Registration Number: NCT03936504.
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http://dx.doi.org/10.1136/bmjopen-2019-036061DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7337900PMC
July 2020

Distribution of Linezolid in Tuberculosis Lesions in Patients with Spinal Multidrug-Resistant Tuberculosis.

Antimicrob Agents Chemother 2020 06 23;64(7). Epub 2020 Jun 23.

Department of Orthopedics, Beijing Friendship Hospital, Capital Medical University, Beijing, China

Linezolid has strong antimicrobial activity against the multidrug-resistant (MDR) strains of Little is known about the distribution of linezolid in tuberculosis (TB) lesions in patients with MDR-TB. The aim of this study was to evaluate the distribution of linezolid in TB lesions in patients with spinal MDR-TB. Nine patients with spinal MDR-TB were enrolled prospectively from August 2019 to February 2020. The patients received a linezolid-containing anti-TB treatment regimen and needed surgery for the removal of TB lesions. During the operation, nine blood samples, eight diseased bone tissue samples, seven pus samples, and four granulation tissue samples were collected simultaneously and 2 h after the oral administration of 600 mg of linezolid. Linezolid concentrations in plasma, diseased bone tissue, pus, and granulation tissue samples were subjected to high-performance liquid chromatography-tandem mass spectrometry. At sample collection, the mean concentrations of linezolid in plasma, diseased bone tissue, pus, and granulation tissue samples of the nine patients were 11.14 ± 5.82, 5.94 ± 4.27, 11.09 ± 4.58, and 14.08 ± 10.61 mg/liter, respectively. The mean ratios of linezolid concentration in diseased bone/plasma, pus/plasma, and granulation/plasma were 53.84%, 91.69%, and 103.57%, respectively. The mean ratios of linezolid concentration in pus/plasma and granulation/plasma were higher than those in diseased bone/plasma, and the difference was statistically significant ( = -2.810,  = 0.015; = -4.901,  = 0.001). In conclusion, linezolid had different concentration distributions in different types of TB-infected tissues in patients with spinal MDR-TB.
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http://dx.doi.org/10.1128/AAC.00450-20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7317991PMC
June 2020

Fibroblast growth factor 19 alleviates palmitic acid-induced mitochondrial dysfunction and oxidative stress via the AMPK/PGC-1α pathway in skeletal muscle.

Biochem Biophys Res Commun 2020 06 16;526(4):1069-1076. Epub 2020 Apr 16.

Department of Geriatrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China. Electronic address:

Obesity-induced fat ectopic deposition results in mitochondrial dysfunction and oxidative stress in skeletal muscle, which could impair the quality and function of the skeletal muscle. Human fibroblast growth factor 19 (FGF19) acts as a vital metabolic regulator of bile acid synthesis and metabolic homeostasis. Recent studies have shown that FGF19 regulates skeletal muscle mass through the enlargement of muscle fiber size and protects muscles from atrophy. However, the role of FGF19 in regulating mitochondrial function and the antioxidant response in skeletal muscle remains unknown. Therefore, we investigated the effect of FGF19 on palmitic acid (PA)-induced mitochondrial dysfunction and oxidative stress in C2C12 cells. In this study, we found that FGF19 can increase the mRNA and protein expression levels of mitochondrial biogenesis regulators (PGC-1α, Nrf-1, and TFAM) and antioxidant response regulators (Nrf-2 and HO-1), alleviating PA-induced mitochondrial dysfunction and oxidative stress. However, the regulatory effect of FGF19 was blocked by Compound C, an AMP-activated protein kinase (AMPK) inhibitor, and siRNA knockdown of PGC-1a. Taken together, these findings indicate that FGF19 might promote mitochondrial biogenesis and antioxidant response via the AMPK/PGC-1α pathway, attenuating the effect of PA on mitochondrial dysfunction and oxidative stress; therefore, FGF19 might be a potential therapeutic target for the effects of obesity on skeletal muscle.
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http://dx.doi.org/10.1016/j.bbrc.2020.04.002DOI Listing
June 2020

Development of a Synergistic Activation Strategy for the Pilot-Scale Construction of Hierarchical Porous Graphitic Carbon for Energy Storage Applications.

ACS Nano 2020 Apr 20;14(4):4741-4754. Epub 2020 Mar 20.

Tianjin International Joint Research Centre of Surface Technology for Energy Storage Materials, College of Physics and Materials Science, Tianjin Normal University, Tianjin 300387, China.

Pursuing scalable production of porous carbon with facile and environmentally friendly synthesis methodology is a global goal. Herein, a unique hierarchical porous graphitic carbon (HPGC) with outstanding textural characteristics is achieved by a special synergistic activation mechanism, in which the low-temperature molten state of polymorphisms can induce a high-rate liquid phase porous activation. HPGC with high specific surface area (SSA, ∼2571 m g) and large pore volume (PV, ∼2.21 cm g) can be achieved, which also possesses the capability to tune textural characteristics (.., SSA, PV, pore size distribution, .) within a wide range. Furthermore, the pilot-scale production of HPGC is accomplished, which shows similar textural characteristics to the lab-scale HPGC. Due to the unique structure of HPGC and the capability of the textural control, it can be applicable in a variety of energy storage, energy conversion, and catalysis applications. The applications of pilot-scale HPGC products in supercapacitors and lithium sulfur batteries are highlighted in this work. Furthermore, the synergistic activation strategy can be promoted to other alkali-based carbon activation routes, which can open up new possibilities for the activated carbon production and lead to more widespread industrialized applications of HPGC.
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http://dx.doi.org/10.1021/acsnano.0c00620DOI Listing
April 2020

Effect of sarcolipin-mediated cell transdifferentiation in sarcopenia-associated skeletal muscle fibrosis.

Exp Cell Res 2020 04 5;389(1):111890. Epub 2020 Feb 5.

Department of Geriatrics, the First Affiliated Hospital of Chongqing Medical University, Friendship Road 1, Yuan Jiagang, 400016, Chongqing, China. Electronic address:

Fibrosis is a key pathological event during muscle aging that accelerates the development of sarcopenia. We show that sarcolipin (SLN) is highly expressed during aging, promotes intracellular calcium overload and participates in impaired myogenic differentiation. d-Galactose (D-gal) was used to induce senescence in C2C12 myoblasts. Conventional AAV-mediated SLN knockdown cells were used to study the role of SLN in muscle physiology and pathophysiology. C2C12 cells were treated with D-gal, which promoted fibrosis and SLN upregulation. The expression of TGF-β1 and α-SMA, which participate in myogenic transdifferentiation, were also elevated. C2C12 cells with reduced sarcolipin expression produced decreased amounts of collagen. Our study identified an unrecognized role of SLN in regulating myogenic transdifferentiation during aging-associated skeletal muscle cell fibrosis. Targeting SLN may be a novel therapeutic strategy to relieve sarcopenia-associated muscle fibrosis.
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http://dx.doi.org/10.1016/j.yexcr.2020.111890DOI Listing
April 2020

An allelic variant of GAME9 determines its binding capacity with the GAME17 promoter in the regulation of steroidal glycoalkaloid biosynthesis in tomato.

J Exp Bot 2020 05;71(9):2527-2536

The Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, China.

Steroidal glycoalkaloids (SGAs) are cholesterol-derived molecules found in the family Solanaceae. SGA content varies among different plant species and varieties. However, the genetic mechanisms regulating SGA content remain unclear. Here, we demonstrate that genetic variation in GLYCOALKALOID METABOLISM 9 (GAME9) is responsible for the variation in SGA content in tomato (Solanum lycopersicum). During a sequential analysis we found a 1 bp substitution in the AP2/ERF binding domain of GAME9. The 1 bp substitution in GAME9 was significantly associated with high SGA content and determined the binding capacity of GAME9 with the promoter of GAME17, a core SGA biosynthesis gene. The high-SGA GAME9 allele is mainly present in S. pimpinellifolium and S. lycopersicum var. cerasiforme populations and encodes a protein that can bind the GAME17 promoter. In contrast, the low-SGA GAME9 allele is mainly present in the big-fruited varieties of S. lycopersicum and encodes a protein that shows weak binding to the GAME17 promoter. Our findings provide new insight into the regulation of SGA biosynthesis and the factors that affect the accumulation of SGA in tomato.
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http://dx.doi.org/10.1093/jxb/eraa014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7210767PMC
May 2020

Rates of periprosthetic infection and surgical revision in Beijing (China) between 2014 and 2016: a retrospective multicenter cross-sectional study.

J Orthop Surg Res 2019 Dec 26;14(1):463. Epub 2019 Dec 26.

Department of Orthopedics, Peking Union Medical College Hospital, CAMS & PUMC, No.1 Shuaifuyuan, Wangfujing, Dongcheng District, Beijing, 100730, China.

Background: Periprosthetic joint infection (PJI) is a rare but devastating complication after total joint arthroplasty. There is a paucity of data on the incidence and prevalence of periprosthetic infection in mainland China. This study aimed to analyze the rates of surgical revision after arthroplasty due to PJI and the procedures followed in Beijing, China.

Methods: The study involved a retrospective multicenter cross-sectional survey of patients undergoing revisions for periprosthetic infection after hip/knee arthroplasty at nine hospitals in Beijing from 2014 to 2016. Age, gender, body mass index, primary diagnosis, comorbidity, primary surgery, treatment methods, and post-revision complications were analyzed.

Results: A total of 38,319 hip/knee arthroplasties and 366 (0.96%) revisions for PJI were identified. Of these, 161 (161/14,110; 1.14%) revisions involved hip arthroplasty, whereas 205 (205/24,209; 0.85%) revisions were due to knee arthroplasty. Procedures for revisions of infected hip included 7 (4.3%) cases of open debridement and prosthesis retention, 32 (19.9%) cases of one-stage exchange, 121 (75.2%) cases of two-stage exchange, and 1 (0.007%) case of hip dissection. As for the infected knee, the procedures included 45 (22.0%) cases of open debridement and prosthesis retention, 13 (6.3%) cases of one-stage exchange, 143 (69.8%) cases of two-stage exchange, and 4 (0.02%) cases of knee fusion.

Conclusions: The study found the rates of revision due to PJI to be low. Nonetheless, the incidence of PJI in mainland China could be higher and calls for more elaborate studies in geographically and socioeconomically diverse health institutions.
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http://dx.doi.org/10.1186/s13018-019-1520-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6933879PMC
December 2019
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