Publications by authors named "Ahmed Ismail"

242 Publications

Role of silver nanoparticles in fluorimetric determination of urea in urine samples.

Spectrochim Acta A Mol Biomol Spectrosc 2022 Jan 11;271:120889. Epub 2022 Jan 11.

Institute of Chemical Sciences, University of Peshawar, Peshawar 25120, Pakistan.

Herein, an economical, analytical and sensitive method was established for the fluorometric determination of urea using freshly prepared silver nanoparticles (Ag-NPs) in real urine samples. The standard addition and second-order derivative methods were selected for the ongoing research work to eliminate the possible effect of interferences in a real environment. In this work, Ag-NPs were prepared by reducing silver nitrate salt in the presence of 1,3-di-(1H-imidazole-1-yl) -2-propanol (DIPO) in an aqueous medium. Urea in the urine samples was successfully determined through the complexation of Ag-NPs with urea molecules. The results revealed high percent recovery with ± RSD of urea in the three different urine samples, where percent recoveries by spectrofluorometric standard addition were 99.77 ± 3.4, 100.24 ± 5.1, 100.93 ± 2.8 and that is by the spectrofluorometric second-order derivative method were 103.57 ± 2.4, 101.8 ± 1.3, 98 ± 3.2, respectively. The successful application of these analytical methods in the spectrofluorometric determination of urea in urine samples can accumulate further addition in the effects and possible role of Ag-NPs in the determination of biological molecules in biological and non-biological samples in the scientific as well as clinical fields.
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http://dx.doi.org/10.1016/j.saa.2022.120889DOI Listing
January 2022

A review of the biological role of miRNAs in prostate cancer suppression and progression.

Int J Biol Macromol 2022 Feb 27;197:141-156. Epub 2021 Dec 27.

Department of Biochemistry, Faculty of Pharmacy, Badr University in Cairo (BUC), Badr City, Cairo 11829, Egypt; Biochemistry Department, Faculty of Pharmacy, Ain-Shams University, Abassia, Cairo 11566, Egypt.

Prostate cancer (PC) is the third-leading cause of cancer-related deaths worldwide. Although the current treatment strategies are progressing rapidly, PC is still representing a substantial medical problem for affected patients. Several factors are involved in PC initiation, progression, and treatments failure including microRNAs (miRNAs). The miRNAs are endogenous short non-coding RNA sequence negatively regulating target mRNA expression via degradation or translation repression. miRNAs play a pivotal role in PC pathogenesis through its ability to initiate the induction of cancer stem cells (CSCs) and proliferation, as well as sustained cell cycle, evading apoptosis, invasion, angiogenesis, and metastasis. Furthermore, miRNAs regulate major molecular pathways affecting PC such as the androgen receptor (AR) pathway, p53 pathway, PTEN/PI3K/AKT pathway, and Wnt/β-catenin pathway. Furthermore, miRNAs alter PC therapeutic response towards the androgen deprivation therapy (ADT), chemotherapy and radiation therapy (RT). Thus, the understanding and profiling of the altered miRNAs expression in PC could be utilized as a non-invasive biomarker for the early diagnosis as well as for patient sub-grouping with different prognoses for individualized treatment. Accordingly, in the current review, we summarized in updated form the roles of various oncogenic and tumor suppressor (TS) miRNAs in PC, revealing their underlying molecular mechanisms in PC initiation and progression.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.12.141DOI Listing
February 2022

New benzoxazole derivatives as potential VEGFR-2 inhibitors and apoptosis inducers: design, synthesis, anti-proliferative evaluation, flowcytometric analysis, and studies.

J Enzyme Inhib Med Chem 2022 Dec;37(1):397-410

Pharmaceutical Medicinal Chemistry & Drug Design Department, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo, Egypt.

A new series of benzoxazole derivatives were designed and synthesised to have the main essential pharmacophoric features of VEGFR-2 inhibitors. Cytotoxic activities were evaluated for all derivatives against two human cancer cell lines, MCF-7 and HepG2. Also, the effect of the most cytotoxic derivatives on VEGFR-2 protein concentration was assessed by ELISA. Compounds , , and showed the highest activities with VEGFR-2 protein concentrations of 586.3, 636.2, and 705.7 pg/ml, respectively. Additionally, the anti-angiogenic property of compound against human umbilical vascular endothelial cell (HUVEC) was performed using a wound healing migration assay. Compound reduced proliferation and migratory potential of HUVEC cells. Furthermore, compound was subjected to further biological investigations including cell cycle and apoptosis analyses. Compound arrested the HepG2 cell growth at the Pre-G1 phase and induced apoptosis by 16.52%, compared to 0.67% in the control (HepG2) cells. The effect of apoptosis was buttressed by a 4.8-fold increase in caspase-3 level compared to the control cells. Besides, different docking studies were also performed to get better insights into the possible binding mode of the target compounds with VEGFR-2 active sites.
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http://dx.doi.org/10.1080/14756366.2021.2015343DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8725875PMC
December 2022

Micro versus Macro Shear Bond Strength Testing of Dentin-Composite Interface Using Chisel and Wireloop Loading Techniques.

Dent J (Basel) 2021 Nov 30;9(12). Epub 2021 Nov 30.

Biomaterials Department, Faculty of Dentistry, Ain Shams University, Cairo 11566, Egypt.

Shear bond strength (SBS) testing is a commonly used method for evaluating different dental adhesive systems. Failure mode analysis provides valuable information for better interpretation of bond strength results. The aim of this study was to evaluate the influence of specimen dimension and loading technique on shear bond strength and failure mode results. Eighty macro and micro flowable composite cylindrical specimens of 1.8 and 0.8 mm diameter, respectively, and 1.5 mm length were bonded to dentinal substrate. Four study groups were created ( = 20): Macroshear wireloop, Gp1; Microshear wireloop, Gp2; Macroshear chisel, Gp3; and Microshear chisel, Gp4. They were tested for SBS using chisel and wireloop loading devices followed by failure mode analysis using digital microscopy and SEM. Two- and one-way ANOVA were used to compare stress at failure values of different groups while the Kruskal-Wallis test was used to compare between failure modes of the tested groups. Gp4 recorded the highest mean stress at failure 54.1 ± 14.1 MPa, and the highest percentage of adhesive failure in relation to the other groups. Specimen dimension and loading technique are important parameters influencing the results of shear bond strength. Micro-sized specimens and chisel loading are recommended for shear testing.
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http://dx.doi.org/10.3390/dj9120140DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8700382PMC
November 2021

Compressive sensing based secure data aggregation scheme for IoT based WSN applications.

PLoS One 2021 16;16(12):e0260634. Epub 2021 Dec 16.

Computer Science Dept., University of Sharjah, Sharjah, UAE.

Compressive Sensing (CS) based data collection schemes are found to be effective in enhancing the data collection performance and lifetime of IoT based WSNs. However, they face major challenges related to key distribution and adversary attacks in hostile and complex network deployments. As a result, such schemes cannot effectively ensure the security of data. Towards the goal of providing high security and efficiency in data collection performance of IoT based WSNs, we propose a new security scheme that amalgamates the advantages of CS and Elliptic Curve Cryptography (ECC). We present an efficient algorithms to enhance the security and efficiency of CS based data collection in IoT-based WSNs. The proposed scheme operates in five main phases, namely Key Generation, CS-Key Exchange, Data Compression with CS Encryption, Data Aggregation and Encryption with ECC algorithm, and CS Key Re-generation. It considers the benefits of ECC as public key algorithm and CS as encryption and compression method to provide security as well as energy efficiency for cluster based WSNs. Also, it solves the CS- Encryption key distribution problem by introducing a new key sharing method that enables secure exchange of pseudo-random key between the BS and the nodes in a simple way. In addition, a new method is introduced to safeguard the CS scheme from potential security attacks. The efficiency of our proposed technique in terms of security, energy consumption and network lifetime is proved through simulation analysis.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0260634PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8675701PMC
January 2022

Detection of TEM, AmpC, SHV, CTX-M, and MCR-1 Genes in Gram Negative Isolates of Urinary Tract Infections.

Clin Lab 2021 Dec;67(12)

Background: Urinary tract infection (UTI) is an infection caused by the presence and growth of microorganisms anywhere in the urinary tract. It is usually due to bacteria from the digestive tract which climb the opening of the urethra and begin to multiply to cause infection. However, UTI is more frequent in female than male, because of the short urethra, absence of prostatic secretion, and pregnancy.

Methods: This study was aimed to detect extended spectrum beta lactamase and MCR-1genes from Gram negative bacterial clinical isolates from urinary tract infections. Ninety-one urine samples were collected in this study, then cultured on CLED agar and identified by conventional biochemical methods. Modified Kirby-Bauer method was used for sensitivity testing. Genomic DNA extracted by boiling method, and multiplex PCR was conducted to amplify TEM, AmpC, MCR-1, SHV, and CTX-M genes from all Gram-negative isolates.

Results: The result of the susceptibility test revealed that the highest resistant rates were 73% for Ceftazidime, followed by 63%, 56%, and 55% for Ciprofloxacin, Gentamicin, and Co-trimoxazole, respectively, and 21% were resistant to Imipenem. Moreover, for the presence of resistance genes, multiplex PCR results displayed that the TEM gene was present in 34% of bacteria, AmpC gene was found in 49.4% of isolates. Also, 38.5% and 6.6% were positive for MCR-1 gene and SHV gene, respectively. All Proteus species were negative to MCR-1 and TEM genes. Fifty E. coli, 7 Klebsiella pneumonia, two Pseudomonas aeruginosa, and five Proteus species were positive for CTX-M gene and all Citrobacter spp. were negative for CTX-M gene. Eighty-nine isolates were positive for one or more ESBL genes, while two isolates were negative to all genes. CTX-M gene is predominant among uropathogenic bacteria and imipenem is the best effective antibiotic.

Conclusions: This recent study proved that the result of the susceptibility test revealed that the highest resistant rate were 73% for Ceftazidime, followed by 63%, 56%, and 55% for Ciprofloxacin, Gentamicin, and Co-trimoxazole, respectively, and 21% were resistant to imipenem.
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http://dx.doi.org/10.7754/Clin.Lab.2021.210315DOI Listing
December 2021

Comparison of antibody immune responses between BNT162b2 and mRNA-1273 SARS-CoV-2 vaccines in naïve and previously infected individuals.

J Travel Med 2021 12;28(8)

Biomedical Research Center, Qatar University, Doha 2713, Qatar.

Background: Two mRNA vaccines, Pfizer-BNT162b2 and Moderna-mRNA-1273, obtained the Emergency Use Listing by WHO for preventing COVID-19. However, little is known about the difference in antibody responses induced by these two mRNA vaccines in naïve and previously infected (PI) individuals.

Method: We investigated the levels of anti-S-RBD (total, IgG and IgA) levels in naïve and PI individuals, 1-13 (median = 6) weeks following the second dose of either vaccine. Results in the naïve-vaccinated group, the mRNA-1273 vaccine induced significantly higher levels of anti-S-RBD total antibodies (3.5-fold; P < 0.001), IgG (2-fold, P < 0.01) and IgA (2.1-fold, P < 0.001) as compared with the BNT162b2 vaccine. In addition, both vaccines produced significantly higher anti-S-RBD total antibody levels in the PI-group compared with naïve-vaccinated group. The PI group elicited a higher level of anti-S-RBD IgG than the naïve-BNT162b2 (P = 0.05), but not more than the naïve-mRNA-1273 (P = 0.9) group. Interestingly, the PI vaccinated group elicited a comparable level of IgA ratio to the naïve-mRNA-1273 group but significantly higher than the naïve-BNT162b2 group (1.6-fold, P < 0.001).

Conclusion: Our results showed that the PI-vaccinated group produces a higher level of antibodies than the naïve vaccinated group, particularly for those vaccinated with BNT162b2.
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http://dx.doi.org/10.1093/jtm/taab190DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8754698PMC
December 2021

New adaptive lasso approaches for variable selection in automated pharmacovigilance signal detection.

BMC Med Res Methodol 2021 Dec 1;21(1):271. Epub 2021 Dec 1.

High-Dimensional Biostatistics for Drug Safety and Genomics, CESP, Université Paris-Saclay, UVSQ, Université Paris-Sud, Inserm, Villejuif, France.

Background: Adverse effects of drugs are often identified after market introduction. Post-marketing pharmacovigilance aims to detect them as early as possible and relies on spontaneous reporting systems collecting suspicious cases. Signal detection tools have been developed to mine these large databases and counts of reports are analysed with disproportionality methods. To address disproportionality method biases, recent methods apply to individual observations taking into account all exposures for the same patient. In particular, the logistic lasso provides an efficient variable selection framework, yet the choice of the regularization parameter is a challenging issue and the lasso variable selection may give inconsistent results.

Methods: We propose a new signal detection methodology based on the adaptive lasso. We derived two new adaptive weights from (i) a lasso regression using the Bayesian Information Criterion (BIC), and (ii) the class-imbalanced subsampling lasso (CISL), an extension of stability selection. The BIC is used in the adaptive lasso stage for variable selection. We performed an extensive simulation study and an application to real data, where we compared our methods to the existing adaptive lasso, and recent detection approaches based on lasso regression or propensity scores in high dimension. For both studies, we evaluate the methods in terms of false discoveries and sensitivity.

Results: In the simulations and the application, both proposed adaptive weights show equivalent or better performances than the other competitors, with an advantage for the CISL-based adaptive weights. CISL and lasso regression using BIC are solid alternatives.

Conclusion: Our proposed adaptive lasso is an appealing methodology for signal detection in pharmacovigilance. Although we cannot rely on test theory, our approaches show a low and stable False Discovery Rate in all simulation settings. All methods evaluated in this work are implemented in the adapt4pv R package.
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http://dx.doi.org/10.1186/s12874-021-01450-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8638444PMC
December 2021

Site-Specific Interrogation of Protein Structure and Stability.

Methods Mol Biol 2022 ;2376:65-87

Department of Chemistry, University of Pennsylvania, Philadelphia, PA, USA.

To execute their function or activity, proteins need to possess variability in local electrostatic environment, solvent accessibility, structure, and stability. However, assessing any protein property in a site-specific manner is not easy since native spectroscopic signals often lack the needed specificity. One strategy that overcomes this limitation is to use unnatural amino acids that exhibit distinct spectroscopic features. In this chapter, we describe several such unnatural amino acids (UAAs) and their respective applications in site-specific interrogation of protein structure and stability using standard biophysical methods, including circular dichroism (CD), infrared (IR), and fluorescence spectroscopies.
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http://dx.doi.org/10.1007/978-1-0716-1716-8_3DOI Listing
January 2022

Potential Importance of Molybdenum Priming to Metabolism and Nutritive Value of spp. Sprouts.

Plants (Basel) 2021 Nov 5;10(11). Epub 2021 Nov 5.

Integrated Molecular Plant Physiology Research, Department of Biology, University of Antwerp, 2020 Antwerpen, Belgium.

Molybdenum ions (Mo) can improve plants' nutritional value primarily by enhancing nitrogenous metabolism. In this study, the comparative effects of seed priming using Mo were evaluated among sproutings of species/cultivars, including (CA1), (CA2), and (CA3). Mo impacts on growth, metabolism (e.g., nitrogen and phenolic metabolism, pigment and total nutrient profiles), and biological activities were assayed. Principal component analysis (PCA) was used to correlate Mo-mediated impacts. The results showed that Mo induced photosynthetic pigments that resulted in an improvement in growth and increased biomass. The N content was increased 0.3-fold in CA3 and 0.2-fold in CA1 and CA2. Enhanced nitrogen metabolism by Mo provided the precursors for amino acids, protein, and lipid biosynthesis. At the secondary metabolic level, phenolic metabolism-related precursors and enzyme activities were also differentially increased in species/cultivars. The observed increase in metabolism resulted in the enhancement of the antioxidant (2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) free radical scavenging, 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), ferric reducing antioxidant power (FRAP)) and antidiabetic potential (Glycemic index (GI) and inhibition activity of α-amylase, and α-glucosidase) of species. The antioxidant activity increased 20% in CA3, 14% in CA1, and 8% in CA2. Furthermore, PCA showed significant variations not only between Mo-treated and untreated samples but also among species. Overall, this study indicated that the sprouts of species have tremendous potential for commercial usage due to their high nutritive value, which can be enhanced further with Mo treatment to accomplish the demand for nutritious feed.
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http://dx.doi.org/10.3390/plants10112387DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8625590PMC
November 2021

Diversity in blueberry genotypes and developmental stages enables discrepancy in the bioactive compounds, metabolites, and cytotoxicity.

Food Chem 2022 Apr 18;374:131632. Epub 2021 Nov 18.

Center for Viticulture and Small Fruit Research, College of Agriculture and Food Sciences, Florida A&M University, Tallahassee, FL 32308, USA. Electronic address:

Eight blueberry cultivars at three developmental stages were investigated for metabolite profiling, antioxidant, and anticancer activities. Cultivars- and developmental stages-variations were determined in total phenolic, flavonoid, DPPH, and FRAP antioxidant assays. The anticancer capacity was equal against A549, HepG2, and Caco-2 cancer cells, whereas the inhibition rate was dose-, incubation period-, cultivar-, and developmental stages-dependent. The untargeted metabolite profiling by UPLC-TOF-MS analysis of two contrast cultivars, 'Vernon' and 'Star', throughout the developmental stages revealed 328 metabolites; the majority of them were amino acids, organic acids, and flavonoids. The multivariate statistical analysis identified five metabolites, including quinic acid, methyl succinic acid, chlorogenic acid, oxoadipic acid, and malic acid, with positively higher correlations with all anticancer activities. This comprehensive database of blueberry metabolites along with anticancer activities could be targeted as natural anticancer potentials. This study would be of great value for food, nutraceutical, and pharmaceutical industries as well as plant biotechnologists.
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http://dx.doi.org/10.1016/j.foodchem.2021.131632DOI Listing
April 2022

Needlestick and sharps injuries among secondary and tertiary healthcare workers, Saudi Arabia.

Nurs Open 2022 Jan 21;9(1):816-823. Epub 2021 Nov 21.

Family and Community Medicine Department, Faculty of Medicine, Jazan University, Jazan City, Jazan, Saudi Arabia.

Aim: The study aimed to assess the incidence of needlestick and sharps injuries among healthcare workers (HCWs) in the Jazan region of Saudi Arabia, as well as to determine whether there exists an association between hospital level and needlestick and sharps injuries rate.

Design: A cross-sectional survey was conducted among 609 randomly selected HCWs from nine general hospitals.

Methods: A self-administered questionnaire, which covered the structure and process of injection safety, was used for data collection.

Results: The overall needlestick and sharps injuries incidence rate was 24%. The needlestick and sharps injuries rates were 30% and 14% in secondary and tertiary hospitals, respectively. HCWs working in tertiary hospitals were 61% less likely to have needlestick and sharps injuries than those employed in secondary hospitals. This was mainly the impact of better and continuous training. High safety level maintenance and health education provision are vital in such settings.
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http://dx.doi.org/10.1002/nop2.1136DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8685775PMC
January 2022

Temperature-Driven Self-Doping in Magnetite.

Phys Rev Lett 2021 Oct;127(18):186402

Debye Institute for Nanomaterials Science, 3584 CG Utrecht, Netherlands.

Magnetite is one of the most fascinating materials exhibiting the enigmatic first-order Verwey transition which is conventionally manipulated through chemical doping. Here, we show that heating magnetite results in a spontaneous charge reordering and, consequently, a hole self-doping effect at the octahedral sublattice. Core-level x-ray spectroscopy measurements combined with theory uncovers that there are three regimes of self-doping that map the temperature dependence of the electrical conductivity and magnetism up to the Curie temperature. Our results provide an elegant analogy between the effect of chemical doping and temperature-driven self-doping on trimerons in magnetite.
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http://dx.doi.org/10.1103/PhysRevLett.127.186402DOI Listing
October 2021

Multidetector computed tomography study to measure thoracic aorta diameters in Egyptian population.

Egypt Heart J 2021 Oct 15;73(1):89. Epub 2021 Oct 15.

Cairo University, Giza, Egypt.

Background: The aorta is the largest and strongest artery in the body that plays an important role in the control of systemic vascular resistance and heart rate. Aortic diseases contribute to the wide spectrum of arterial diseases that may be diagnosed after a long period of subclinical development. Multidetector computed tomographic scanners (≥ 64 detector rows) for aortic imaging remain one of the most preferred imaging techniques for diagnosis and follow-up of aortic conditions in acute as well as chronic presentations. The aim of this study is to establish a normal reference values for aortic diameters among Egyptian population and to find which of the cardiovascular risk factors could be an independent determinant of the aortic diameters.

Results: Five hundred and sixteen Egyptian individuals were enrolled in our study, the mean age was 53.5 ± 10.9, and males comprised 61.4% of the study population. Aortic root diameters measured at the annulus, sinus and STJ were 23.09 ± 2.55 mm, 33.75 ± 3.93 mm and 26.13 ± 3.05 mm, respectively. The BSA-indexed diameters were 11.70 ± 1.39, 17.10 ± 2.10 and 13.25 ± 1.65, respectively. The diameter of the tubular part of ascending aorta was 30.97 ± 4.16 mm, and the BSA-indexed diameter was 15.71 ± 2.28. The aortic diameters measured at the level of the pulmonary bifurcation were 24.56 ± 2.95 mm and 23.79 ± 2.96 mm at systolic and diastolic phases, respectively. The BSA-indexed diameters were 12.44 ± 1.52 and 12.05 ± 1.52 at systolic and diastolic phases, respectively. At the diaphragmatic level, the mean diameters were 22.39 ± 2.72 mm and 21.49 ± 2.79 mm at systolic and diastolic phases, respectively. The BSA-indexed diameters were 11.34 ± 1.43 and 10.98 ± 1.48 at systolic and diastolic phases, respectively. Age, gender, BSA, BMI and hypertension were statistically significant independent predictors of ascending and descending aortic diameters.

Conclusions: Our study established a normal reference value for thoracic aortic diameters among Egyptians using contrast enhanced MSCT aortography. Age, Gender, BSA, BMI and hypertension are the major determinants of aortic diameters.
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http://dx.doi.org/10.1186/s43044-021-00216-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8519996PMC
October 2021

Serratus Anterior Plane Block in Pediatric Patients Undergoing Thoracic Surgeries: A Randomized Controlled Trial.

J Cardiothorac Vasc Anesth 2021 Sep 22. Epub 2021 Sep 22.

Anesthesia and Pain Management, Department of Anesthesia, Kasr Alainy Faculty of Medicine, Cairo University, Egypt. Electronic address:

Objective(s): This study was designed to evaluate the efficacy and safety of serratus anterior plane block (SAPB) as an analgesic technique for thoracotomies in pediatric patients.

Design: Double-blinded randomized controlled trial.

Setting: A single-center study at Aboelrish Pediatric Hospital, one tertiary hospital of Cairo University Hospitals.

Participants: Seventy pediatric patients aged six months-to-three years scheduled for thoracotomies.

Interventions: Patients were randomized into two groups, group SF and group F. Group SF received an ultrasound-guided SAPB (n = 35), whereas group F (n = 35) did not. All groups received an intraoperative fentanyl infusion (at 0.5 μg/kg /h).

Measurements: The primary outcome was the total dose of postoperatively administrated fentanyl in the first 24 hours. The secondary outcomes included the total dose of intraoperative additional fentanyl boluses; time of the first postoperative rescue analgesia; and postoperative Face, Legs, Activity, Cry, Consolability scale (FLACC) score values.

Main Results: The main results of this study showed that the administrated fentanyl in the 24 hours postoperatively was significantly lower in SF group than in F group (p value ˂ 0.001). In addition, significant decreases of the postoperative FLACC pain score (p value ˂ 0.001), reduction of intraoperative fentanyl consumption (p value ˂ 0.001), and delay of the first rescue analgesia (p value ˂ 0.001) were recorded in SF group in relation to F group without significant complications in both groups.

Conclusions: Serratus anterior plane block can provide a safe, effective, and easy-to-perform regional technique for children undergoing thoracotomies.
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http://dx.doi.org/10.1053/j.jvca.2021.09.014DOI Listing
September 2021

Computerized tomography of the Thorax for surgical patients during the COVID-19 pandemic: Was it useful?

Int J Clin Pract 2021 Nov 12;75(11):e14774. Epub 2021 Sep 12.

Consultant Colorectal Surgery, New Cross Hospital, Wolverhampton, UK.

Objectives: Diagnostic challenges during the corona virus disease (COVID-19) pandemic forced the radiology regulating body to adopt the use of CT Chest as a triage and diagnostic tool, which was subsequently abandoned. The Royal Wolverhampton hospital followed both protocols. Here, we investigate the evidence behind this decision within the context of surgical admissions during the COVID-19 peak in our hospital.

Methods: Retrospective data collection and analysis of all surgical admissions between the 1st of March to the 31st of May. Data were collected from the radiology and electronic portal looking into patients undergoing CT chest to diagnose the presence of COVID-19 as well as swab results.

Results: Seventy-eight patients fulfilled our inclusion criteria. The scan either confirmed the presence or absence (4, 63 patients) of COVID-19 but was sometimes inconclusive (11 patients). Comparing these to the results of the swabs; CT showed sensitivity 42.86%, Specificity 97.92%, and accuracy 90.91%. In the inconclusive CT report group, chances of having a positive swab result were 45%: None of the scan results changed any of the surgical planning. Lymphocyte count in the context of surgical presentation did not have any statistical significance to predict the presence of COVID-19 (P = .7). Cost implications on our cohort of patients for adding the chest CT is estimated to be around £31 000.

Conclusion: CT Thorax during the pandemic was a good negative predictor but had limited diagnostic value and did not change patient management. Newer, faster techniques of PCR swabs and antibody testing would be a better and cheaper alternative.
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http://dx.doi.org/10.1111/ijcp.14774DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8646394PMC
November 2021

Evaluation of Efficacy and Lateral Gaze Incomitance in Symmetrical and Asymmetrical Surgery for Concomitant Esotropia and Exotropia.

Clin Ophthalmol 2021 26;15:3613-3621. Epub 2021 Aug 26.

Ophthalmology Department, Faculty of Medicine, Minia University, Minia, Egypt.

Purpose: Evaluation of asymmetrical and symmetrical horizontal strabismus surgery as regards efficacy and postoperative lateral gaze incomitance.

Patients And Methods: This prospective comparative interventional case series study included 40 patients of age more than 3 years with alternating horizontal strabismus. Patients were divided according to the type of horizontal deviation into esotropia and exotropia groups, which were further subdivided into asymmetrical and symmetrical subgroups in each type. The surgery was defined as symmetrical procedure if the surgery was done on the same muscle in the two eyes and it was defined as asymmetric in recess-resect procedures in one eye, and three horizontal muscles surgery. Preoperative and postoperative measurements were done in primary position, right, and left gaze using alternate prism cover test and hand-held orthopedic goniometer.

Results: At the end of follow-up period, a statistically insignificant difference (P value = 0.8057) was present in the success rate between the asymmetrical and symmetrical subgroups of esotropia (90 and 86.67%, respectively) and it was 100% in both exotropia subgroups. Lateral gaze incomitance results were different between esotropia and exotropia subgroups. In esotropia, a statistically significant difference was reported at 1 month (0.009), which became insignificant at 6 months (0.077) and 12 months (0.077) between asymmetrical and symmetrical subgroups. In exotropia subgroups, there was a statistically insignificant difference in lateral gaze incomitance at 1, 6, and 12 months (P = 1).

Conclusion: Asymmetrical procedure had the same efficacy and success rate of symmetrical procedure for the correction of concomitant horizontal deviations in primary gaze without persistent postoperative lateral gaze incomitance, especially in exotropia.

Clinical Trial Registration: NCT04199286.
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http://dx.doi.org/10.2147/OPTH.S326659DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8405227PMC
August 2021

A System Identification and Implementation of a Soft Sensor for Freeform Bending.

Materials (Basel) 2021 Aug 13;14(16). Epub 2021 Aug 13.

Integrity of Materials and Structures (IMS), Department of Ferrous Metallurgy (IEHK), RWTH Aachen University, Intzestraße 1, 52072 Aachen, Germany.

The primary goal of this study is the formulation of a soft sensor that predicts industrially relevant mechanical properties for freeform bending. This serves as the foundation of a closed-loop property control. It is hypothesized that by inline measurement of hardness, predictions regarding residual hoop stresses, local strength and strain level can be achieved. A novel hardness-based correlation scheme is introduced, which is implemented into an extended Kalman filter (EKF) and allows an inline prediction of local strength, residual hoop stresses and plasticity. Furthermore, the ultrasonic contact impedance (UCI) method is validated as a suitable inline measuring solution.
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http://dx.doi.org/10.3390/ma14164549DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8398061PMC
August 2021

Complete Genome Sequences of Bacillus cereus Group Phages AaronPhadgers, ALPS, Beyonphe, Bubs, KamFam, OmnioDeoPrimus, Phireball, PPIsBest, YungSlug, and Zainny.

Microbiol Resour Announc 2021 Aug 19;10(33):e0030021. Epub 2021 Aug 19.

Center for Biological Data Science, Virginia Commonwealth University, Richmond, Virginia, USA.

Here, we report genome sequences of 10 Bacillus cereus group-infecting bacteriophages. Each virus was isolated from an environmental sample, contained a double-stranded DNA genome, and belonged to the family. Nine phages exhibit a conserved genome structure, and one phage appears novel in genome structure, sequence, and protein content.
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http://dx.doi.org/10.1128/MRA.00300-21DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8375487PMC
August 2021

compensates for in mice in the amino-terminal acetylation pathway.

Elife 2021 08 6;10. Epub 2021 Aug 6.

Stanley Institute for Cognitive Genomics, Cold Spring Harbor Laboratory, Woodbury, United States.

Amino-terminal acetylation is catalyzed by a set of N-terminal acetyltransferases (NATs). The NatA complex (including X-linked Naa10 and Naa15) is the major acetyltransferase, with 40-50% of all mammalian proteins being potential substrates. However, the overall role of amino-terminal acetylation on a whole-organism level is poorly understood, particularly in mammals. Male mice lacking show no globally apparent in vivo amino-terminal acetylation impairment and do not exhibit complete embryonic lethality. Rather nulls display increased neonatal lethality, and the majority of surviving undersized mutants exhibit a combination of hydrocephaly, cardiac defects, homeotic anterior transformation, piebaldism, and urogenital anomalies. is a previously unannotated -like paralog with NAT activity that genetically compensates for . Mice deficient for have no apparent phenotype, whereas mice deficient for and display embryonic lethality. The discovery of adds to the currently known machinery involved in amino-terminal acetylation in mice.
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http://dx.doi.org/10.7554/eLife.65952DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8376253PMC
August 2021

Can commercial automated immunoassays be utilized to predict neutralizing antibodies after SARS-CoV-2 infection? A comparative study between three different assays.

Front Biosci (Landmark Ed) 2021 07;26(7):198-206

Biomedical Research Center, Qatar University, 2713 Doha, Qatar.

: High-throughput assays that can infer neutralizing activity against SARS-CoV-2 are of great importance for assessing the immunity induced by natural infection and COVID-19 vaccines. We aimed to evaluate the performance and degree of correlation of three fully automated anti-SARS-CoV-2 immunoassays with neutralization activity using a surrogate virus-neutralizing test (sVNT) from GenScript, targeting the receptor-binding domain. : 110 sera collected from PCR-confirmed asymptomatic COVID-19 individuals were tested for neutralizing antibodies (nAbs) using the sVNT. Positive samples were tested on three automated immunoassays targeting different viral antigens: Mindray CL-900i®, Abbott Architect, and Ortho VITROS®. The diagnostic sensitivity, specificity, agreement, and correlation with the sVNT were assessed. Receiver operating characteristic (ROC) curve analysis was performed to determine optimal thresholds for predicting the presence of neutralizing activity by each assay. : All three assays showed 100% specificities. The highest sensitivity was 99.0%, demonstrated by VITROS®, followed by 94.3%, for CL-900i®, and 81.0%, for Architect. Both VITROS® and CL-900i® had the strongest correlation with the sVNT (ρ = 0.718 and ρ = 0.712, respectively), while Architect showed a moderate correlation (ρ = 0.618). ROC curve analysis indicated that the manufacturer's recommended cutoff values are adequate for predicting the presence of nAbs and providing a strong correlation with the sVNT. : VITROS® and CL-900i® serological assays, which detect antibodies against SARS-CoV-2 spike protein, could serve as reliable assays to predict neutralization activity after infection or vaccination.
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http://dx.doi.org/10.52586/4934DOI Listing
July 2021

Efficacy of Dexmedetomidine Versus Morphine as an Adjunct to Bupivacaine in Caudal Anesthesia for Pediatric Thoracic Surgeries: A Randomized Controlled Trial.

Anesth Pain Med 2021 Apr 3;11(2):e112296. Epub 2021 May 3.

Lecturer of Anesthesia and Pain Management, Anesthesia Department, Faculty of Medicine, Cairo University, Cairo, Egypt.

Background: Caudal anesthesia is an effective method of pain management, which can be successfully employed to minimize post-thoracotomy pain in pediatric patients. However, its main disadvantage is the short postoperative analgesic period, which can be prolonged by the concurrent administration of one of many adjuvants.

Objectives: This prospective randomized, blinded study aimed to compare the efficacy of dexmedetomidine versus morphine as adjuvants to bupivacaine in caudal anesthesia for thoracic surgeries in pediatric patients.

Methods: Fifty patients were randomly allocated into two equal groups. To achieve caudal epidural block anesthesia, the patients in group M (n = 25) were administered morphine and bupivacaine, while group D (n = 25) received a mixture of dexmedetomidine and bupivacaine. The primary outcome of this study was the postoperative analgesic duration achieved. The secondary outcomes included morphine administration in the first 24 hours following caudal block anesthesia, the face, legs, activity, cry, consolability (FLACC) scale scores, and adverse effects, including vomiting, itching, bradycardia, hypotension, and respiratory depression.

Results: The results showed that patients who had received dexmedetomidine achieved a longer postoperative analgesia as compared to those who had received morphine (P < 0.001). Postoperatively, the heart rate, blood pressure, pain score, and mean consumption of morphine were lower in group D as compared to the group M. There was no significant difference in the adverse effects between the two groups.

Conclusions: The use of dexmedetomidine as an adjuvant to bupivacaine for caudal anesthesia during pediatric thoracic surgeries induced better and prolonged postoperative analgesia as compared to morphine.
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http://dx.doi.org/10.5812/aapm.112296DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8314090PMC
April 2021

A randomised controlled study of face-to-face versus internet-based teaching of CBT skills for healthcare professionals in Sudan.

BJPsych Int 2021 May;18(2):E7

Psychological Wellbeing Practitioner, Insight Healthcare, Insight Medway, UK.

Background: A need has been identified for affordable and scalable methods for disseminating cognitive-behavioural therapy (CBT) to countries with barriers to traditional methods of teaching.

Objective: This study evaluated the effectiveness of internet-based teaching versus face-to-face teaching in improving the CBT theory, assessment, and formulation skills of a group of mental health practitioners in Khartoum, Sudan.

Method: Participants ( = 36) were randomly assigned to (a) a 3 h live lecture, or (b) a computer-based multimedia recorded lecture of the same duration. Participants were rated before and after training for their ability to assess a simulated patient and construct a CBT formulation of the presenting difficulties. Participants also rated the feasibility and acceptability of the training they had received.

Results: Both teaching methods resulted in significant improvements in participants' abilities to carry out the assessment and formulation tasks. However, participants allocated to computer-based teaching performed better than those allocated to live teaching (between-groups effect size = 0.26-0.74). Both teaching methods were rated as highly acceptable and feasible by participants.

Conclusions: Computer-based teaching could offer a cheaper and effective method to help disseminate CBT to countries with limited resources and expertise, replacing and supplementing other costly traditional methods such as face-to-face teaching.
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http://dx.doi.org/10.1192/bji.2020.60DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8274431PMC
May 2021

Determination of Fertility-Related Traits in Muscadine Grape Population.

Plants (Basel) 2021 Jun 9;10(6). Epub 2021 Jun 9.

Center for Viticulture and Small Fruit Research, College of Agriculture and Food Sciences, Florida A&M University, Tallahassee, FL 32308, USA.

In this study, fertility-related traits of 90 muscadine grape genotypes were evaluated. Selected genotypes included 21 standard cultivars, 60 breeding lines, and nine hybrids (VM hybrids). The first fruiting bud (FFB), bud fertility (BF), bud fertility coefficient (BFC), number of flowers/flower cluster (N.F/FC), fruit-set efficiency (FSE), number of clusters/vine (N.C/V), and yield/vine (Y/V) traits were evaluated. The FFB trait did not show significant differences among genotypes. The muscadine genotype O28-4-2-2 (1.6 ± 0.2) displayed the FFB closest to the base; however, O17-16-2-1, O18-2-1, and VM A12-10-2 genotypes had the most distant FFB (3.6 ± 0.3). All the other fertility-related traits varied widely among the population. The BF, BFC, N.F/FC, FSE, N.C/V, and Y/V exhibited a range estimated at 35.1%, 81.5%, 259.7, 63.3%, 177 C/V, and 22.3 kg/V, respectively. The muscadine genotypes O42-3-1 (36.7% ± 1.3) and Majesty (34% ± 1.2) exhibited the highest BF; however, the VM A12-10-2 (1.6% ± 0.1) recorded the lowest BF. The VM genotype O15-16-1 (82.8% ± 4.1) displayed the highest BFC; however, the VM A12-10-2 (1.3% ± 0.1) showed the lowest BFC. The muscadine genotypes D7-1-1 (280.3 F/FC ± 21.7) and O17-17-1 (20.7 F/FC ± 5.5) showed the highest and lowest N.F/FC, respectively. The maximum and minimum FSE was observed for the Rosa cultivar (65.7% ± 2.4) and muscadine genotype D7-1-1 (2.4% ± 0.2), respectively. The minimum N.C/V was recorded for VM genotype A12-10-2 (6 C/V ± 0.2) and maximum noted for muscadine genotypes B20-18-2 (183 C/V ± 7.5) and O44-14-1 (176 C/V ± 7.3). Muscadine genotype O23-11-2 (22.6 kg ± 1.1) produced the highest Y/V; however, the lowest yield was recorded for O15-17-1, Fry Seedless, Sugargate, and the VM genotypes and A12-10-2, with an average yield among them estimated at 0.4 kg ± 0.2.
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http://dx.doi.org/10.3390/plants10061175DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227160PMC
June 2021

Acute and Cumulative Effects of Repeated Exposure to Chlorpyrifos on the Liver and Kidney Function among Egyptian Adolescents.

Toxics 2021 Jun 10;9(6). Epub 2021 Jun 10.

Department of Occupational and Environmental Health, College of Public Health, University of Iowa, Iowa City, IA 52242, USA.

Background: There is a paucity of research that tracks changes in liver and kidney function among pesticide applicators. The aim of the current study was to investigate the role of repeated seasonal exposure to the organophosphorus pesticide, chlorpyrifos, on serum measures of liver and kidney function.

Methods: Pesticide exposure was assessed by measuring the urinary concentrations of 3,5,6-trichloro-2 pyridinol (TCPy), a specific biomarker for chlorpyrifos. Chlorpyrifos exposure and 8 serum markers of liver and kidney function were measured at 15 timepoints over 3 years prior to, during, and following the end of seasonal pesticide application among adolescent applicators and non-applicators from 4 field stations in Menoufia, Egypt.

Results: Urinary TCPy levels showed increases during the application cycles and recovery at the end of each application season. Altered serum markers of liver and kidney function were associated with chlorpyrifos exposure, with some markers recovering 3 months after the end of exposure each year, while other measures demonstrated progressive increase up to 300% the baseline levels at the end of 3 years.

Conclusion: The study demonstrated that frequent assessment of liver and kidney function is a sound practice to evaluate cellular injury following chronic repeated occupational and environmental exposure to chlorpyrifos.
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http://dx.doi.org/10.3390/toxics9060137DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8230541PMC
June 2021

Untargeted Metabolomics and Antioxidant Capacities of Muscadine Grape Genotypes during Berry Development.

Antioxidants (Basel) 2021 Jun 4;10(6). Epub 2021 Jun 4.

Center for Viticulture and Small Fruit Research, College of Agriculture and Food Sciences, Florida A&M University, Tallahassee, FL 32308, USA.

Three muscadine grape genotypes ( (Michx.) Small) were evaluated for their metabolite profiling and antioxidant activities at different berry developmental stages. A total of 329 metabolites were identified using UPLC-TOF-MS analysis (Ultimate 3000LC combined with Q Exactive MS and screened with ESI-MS) in muscadine genotypes throughout different developmental stages. Untargeted metabolomics study revealed the dominant chemical groups as amino acids, organic acids, sugars, and phenolics. Principal component analysis indicated that developmental stages rather than genotypes could explain the variations among the metabolic profiles of muscadine berries. For instance, catechin, epicatechin-3-gallate, and gallic acid were more accumulated in ripening seeds (RIP-S). However, tartaric acid and malonic acid were more abundant during the fruit-set (FS) stage, and malic acid was more abundant in the veraison (V) stage. The variable importance in the projection (VIP > 0.5) in partial least-squares-discriminant analysis described 27 biomarker compounds, representing the muscadine berry metabolome profiles. A heatmap of Pearson's correlation analysis between the 27 biomarker compounds and antioxidant activities was able to identify nine antioxidant determinants; among them, gallic acid, 4-acetamidobutanoic acid, trehalose, catechine, and epicatechin-3-gallate displayed the highest correlations with different types of antioxidant activities. For instance, DPPH and FRAP conferred a similar antioxidant activity pattern and were highly correlated with gallic acid and 4-acetamidobutanoic acid. This comprehensive study of the metabolomics and antioxidant activities of muscadine berries at different developmental stages is of great reference value for the plant, food, pharmaceutical, and nutraceutical sectors.
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http://dx.doi.org/10.3390/antiox10060914DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8230005PMC
June 2021

Low-Dose Ketamine Infusion Versus Morphine Infusion During Abdominoplasty to Change the Postoperative Pain Profile.

Anesth Pain Med 2020 Dec 19;10(6):e108469. Epub 2020 Dec 19.

Anesthesia Department, Cairo University, Cairo, Egypt.

Background: With the increased number of abdominoplasty all over the world, and the need to manage postoperative pain, it is a must to find proper and effective drugs to decrease opioid consumption in the postoperative period.

Objectives: In this double-blind randomized controlled clinical trial, we assumed that low-dose ketamine infusion will reduce the postoperative pain profile than the conventional method of morphine.

Methods: The scheduled patients for abdominoplasty under general anesthesia were recruited in two groups: group (K) with low-dose ketamine infusion intra-operatively (80 patients) and group (M) with morphine infusion intra-operatively (80 patients). Both groups were monitored intraoperatively and postoperatively for rescue doses of fentanyl, visual analogue scale (VAS), and side effects.

Results: There were no statistical differences between both groups regarding the fentanyl rescue doses intra- and postoperative with no remarkable side effects.

Conclusions: Low-dose ketamine has a useful analgesic effect in abdominoplasty similar to morphine without remarkable side effects, such as sedation or hallucinations.
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http://dx.doi.org/10.5812/aapm.108469DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8207844PMC
December 2020

Mental Stress in Anesthesia and Intensive Care Physicians During COVID-19 Outbreak.

Anesth Pain Med 2020 Oct 1;10(5):e106623. Epub 2020 Sep 1.

Anesthesia Department, Cairo University, Giza, Egypt.

Background: Since the announcement of the World Health Organization of COVID-19 as a global pandemic, the probability of experiencing psychological disturbances and mental health problems among the frontline doctors who deal early with the suspected or confirmed patients is expected to increase, especially among anesthesia and ICU physicians.

Objectives: The objective of this study was to assess the magnitude of mental stress among anesthesia and ICU physicians in Cairo university hospitals, Egypt, treating patients exposed to COVID-19.

Methods: This cross-sectional, survey-based study collected the demographic and mental health data from anesthesia and ICU physicians working in Cairo university hospitals from March 15 to April 15, 2020, in Egypt. The level of stress was assessed by the perceived stress scale (PSS-10).

Results: A total of 193 out of 315 contacted physicians completed the survey. A significant proportion of participants (65%) experienced high levels of psychological distress during this acute situation, with the PSS-10 median score of 21 in the registrar group and 18 in the consultant group; the difference was statistically significant (P = 0.03). The most common causes of stress among the participants were the fears that they might transmit the disease to their loved people and the lack of a clear protocol on how to deal with COVID-19 cases, either suspected or confirmed.

Conclusions: In this survey study, anesthesia and ICU physicians responding to the spread of COVID-19 reported high rates of symptoms of mental stress.
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http://dx.doi.org/10.5812/aapm.106623DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8207887PMC
October 2020

Biometrics Assessment of Cluster- and Berry-Related Traits of Muscadine Grape Population.

Plants (Basel) 2021 May 26;10(6). Epub 2021 May 26.

Center for Viticulture and Small Fruit Research, College of Agriculture and Food Sciences, Florida A&M University, Tallahassee, FL 32308, USA.

In this study, biometrics assessment of flower structure, cluster-, and berry-related traits were evaluated in a population of 90 muscadine grape genotypes for three consecutive years. This population consisted of 21 standard cultivars, 60 breeding lines, and 9 hybrids (VM hybrids). Cluster length (CL) and width (CWI) characteristics exhibited slight differences among the population, with a range estimated at 7.1 and 4.6 cm, respectively. However, cluster weight (CWE), number of berries/cluster (N.B/C), and cluster compactness (CC) traits showed more diversity between individuals with a calculated range of 205.6 g, 32.6 B/C, and 24.1, respectively. Interestingly, all berry-related traits greatly varied between individuals, excluding the number of seeds/berry (N.S/B) character. The N.S/B trait displayed a narrow range of 5.6 seeds within the population. However, characters of berry length (BL), width (BWI), weight (BWE), the weight of seeds/berry (W.S/B), firmness (FF), and dry scar pattern (SP) demonstrated a wide estimated range of 21.2 mm, 21.7 mm, 25.4 g, 0.71 g, 0.21 N, and 82%, respectively. Normal distribution analysis for each trait suggested different distribution patterns extended between unimodal to multimodal behavior. Hierarchical mapping analysis was able to classify the population into several clades based on physical cluster- and berry-related attributes. The PCA suggested that hermaphroditic (perfect) flower structure is associated with compact clusters exhibiting small berries in size and weight (i.e., muscadine genotypes suitable for wine production). However, female flower structure is associated with clusters displaying large berries in size and weight (i.e., muscadine genotypes appropriate for fresh consumption). These patterns occurred independently of cluster size and weight characters. This research is the first study evaluating muscadine biometrics characters at a population level, providing valuable information for market demand and muscadine breeding programs.
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http://dx.doi.org/10.3390/plants10061067DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227436PMC
May 2021

Evaluation of Biochemical Juice Attributes and Color-Related Traits in Muscadine Grape Population.

Foods 2021 May 16;10(5). Epub 2021 May 16.

Center for Viticulture and Small Fruit Research, College of Agriculture and Food Sciences, Florida A&M University, Tallahassee, FL 32308, USA.

Biochemical juice attributes and color-related traits of muscadine grape genotypes have been investigated. For this study, 90 muscadine genotypes, including 21 standard cultivars, 60 breeding lines, and 9 hybrids (VM), were evaluated. The biochemical properties of total soluble solids (TSS), titratable acidity, and TSS/Acid (T/A) ratio showed modest diversity among genotypes with a range of 10.3 °Brix, 2.1 mg tartaric acid/L, and 4.6, respectively. Nonetheless, the pH trait exhibited a tight range of 0.74 among the population with a minimum and maximum pH of 3.11 ± 0.12 and 3.85 ± 0.12. Color-related traits showed more deviation between individuals. Total anthocyanin content (TAC), luminosity index (L*), hue angle (°), and chroma index (C*) displayed a range of 398 µg/g DW, 33.2, 352.1, and 24, respectively. The hierarchical clustering map classified the population into two large groups of colored and non-colored grapes based on L* and °, suggesting the predominance of these two characters among the population. The colored berries genotypes clade was further divided into several sub-clades depending on C*, TAC, and TSS levels. The principal component analysis (PCA) separated the four-color characteristics into two groups with a negative correlation between them, L* and C* versus TAC and °. Further, PCA suggested the positive influence of acidity in enhancing the different nutraceutical components. Despite the nature of anthocyanins as a member of phenolic compounds, a lack of significant correlation between TAC and nutraceutical-related traits was detected. The dissimilatory matrix analysis highlighted the muscadine individuals C11-2-2, E16-9-1, O21-13-1, and Noble as particular genotypes among the population due to enhanced color characteristics.
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http://dx.doi.org/10.3390/foods10051101DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8156615PMC
May 2021
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