Dr Afolayan Adedotun Onoyinka, PhD - National Center for Genetic Resources and Biotechnology (NACGRAB) - Dr

Dr Afolayan Adedotun Onoyinka

PhD

National Center for Genetic Resources and Biotechnology (NACGRAB)

Dr

Ibadan, oyo | Nigeria

Main Specialties: Biology, Biotechnology, Other, Public Health

Additional Specialties: Ecology and Environmental Biology

ORCID logohttps://orcid.org/0000-0002-7678-2964


Top Author

Dr Afolayan Adedotun Onoyinka, PhD - National Center for Genetic Resources and Biotechnology (NACGRAB) - Dr

Dr Afolayan Adedotun Onoyinka

PhD

Introduction

Dr. Adedotun Onoyinka AFOLAYAN graduated with PhD (Environmental Biology) from University of Ibadan, Nigeria. She is currently an Assistant Chief Scientific Officer and Head of Tissue Culture Section, Biotechnology Unit, National Centre for Genetic Resources and Biotechnology (NACGRAB), Nigeria. She is also the Vice-President of the Nigeria Chapter of Society for Conservation Biology. She is a Research Fellow and member of the Nigerian Conservation Foundation. She is an Alumnus of NUFFIC and Fellow, Netherlands Fellowship Program (NFP). She is also a Present Member of Biotechnology Society of Nigeria (BSN), Association for Environmental Impact Assessment of Nigeria (AEIAN), Horticultural Society of Nigeria (HORTSON) among others.
Dr. Afolayan has worked with both the academic and research organizations. She has contributed positively to both the intellectual and research resources of the nation. She was Assistant Lecturer at the School of Medical Laboratory, University College Hospital (UCH), Nigeria (2006 – 2008) where she taught the degree students Biology. She then proceeds to carrying out independent research studies at the department of Zoology, University of Ibadan, towards the award of a Doctor of Philosophy degree. She got appointment at NACGRAB in 2011 as Scientific Officer 1; and has been at this Institution since that time. She is diligent and has an indisputable passion for research, a unique quality that has contributed positively to the achievement of the given mandates of the Institution.
Dr. Afolayan’s interest is in Tissue Culture Techniques; Tissue Culture Media Preparation (Protocol research and design, modification and application); Environmental Monitoring and Assessment of Polluted Communities; Pollution Evaluations (Aquatic, Terrestrial and Air); Heavy Metal Analysis in Biotic and Abiotic Systems; Water Quality Assessment; Biodiversity Monitoring, Biodiversity Conservation among others.
Dr. Afolayan’s passion for research has led her to different collaborative research studies at National and International level. She was selected as the team coordinator for Team AMKID under a collaborative research grant from the International Foundation for Science (IFS), Sweden (2015). Through the collaboration of International Institute for Tropical Agriculture (IITA) with NACGRAB, she has also contributed immensely to the ongoing project on Yam Improvement for Income and Food Security in West Africa (YIIFSWA), under the project leadership of Dr. Norbert Maroya (IITA) and research coordination of Dr. Morufat Balogun. This project, which is sponsored by Bill and Melinda Gates, has given IITA the responsibility to improve yam quality for farmer’s benefit so as to ensure food security in West Africa and the Tissue Culture Section of NACGRAB is saddled with the responsibility of the rapid in vitro multiplication of the yam germplasm. Being the head of this section and the lead researcher, she has passionately seen to the successful delivery of the given responsibility. She also corresponds for IITA in this project as the authorized desk officer.
Dr. Afolayan has taught, supervised and mentored Degree, Masters and Doctoral students in Nigeria. She also has published different scientific articles in journals internationally. She has also been involved in training both students and researchers in different organized national and international training workshops. Being a dynamic person with great enthusiasm and a high sense of purpose, Dr. Afolayan is an active team worker with a great sense of commitment and an immense ability to get things done. She has quite commendable leadership ability and demonstrates a good level of discipline. In 2016, she was given the IBRD.IDA/World Bank’s letter of appreciation for her contribution in promoting and enabling the Business of Agriculture.
Dr. Afolayan is an ecologist who believes in conservation of the natural resources. Her research studies cut across monitoring pollution levels and impacts (with the aim of protecting the residents of the polluted community from exposure to toxic pollutants in the environment); preventing over-exploitation of fish resources using plant extracts; rapid multiplication of endangered bio-resources for conservation using Tissue Culture Techniques among others. Presently, at her place of work, she is currently working with faithful researchers in ensuring that the nation’s immense natural plant resources are conserved through the use of tissue culture techniques. Her passion for biodiversity conservation is what led her to team with Dr. F. Babalola in serving humanity at local level through Save Sahara Network (SSN).
Dr. Afolayan is a devoted Christian, happily married and blessed. She currently serves humanity as the leader and wife of the resident pastor of Pilgrim Miracle Church. She also mentored little children in the Junior Church of the same Ministry, coaching them to build a life of honesty, integrity, contentment, excellence and righteousness.

Primary Affiliation: National Center for Genetic Resources and Biotechnology (NACGRAB) - Ibadan, oyo , Nigeria

Specialties:

Additional Specialties:

Research Interests:


View Dr Afolayan Adedotun Onoyinka’s Resume / CV

Education

Aug 2016
University of Ibadan
PhD
PhD in Ecology and Environmental Biology
Aug 2016
University of Ibadan
PhD
PhD in Ecology and Environmental Biology
Jan 2006
University of Ibadan
MSc
MSc in Ecology and Environmental Biology
Nov 2002
University of Ibadan
BSc.
BSc. in Zoology

Experience

May 2015
International Foundation for Science Collaborative Grant
Team Coordinator/Project investigator
Project Team Name is TEAM AMKID

Publications

11Publications

-Reads

34Profile Views

112PubMed Central Citations

Utilization of AAS and GIS Tools in Assessment of Distribution of Pb, Cd, Cr and Mn Concentrations Within Top-Soil of An Automobile Workshop Within University College Hospital, Ibadan, Nigeria

AFOLAYAN Adedotun Onoyinka, POPOOLA Shola Caleb "Utilization of AAS and GIS Tools in Assessment of Distribution of Pb, Cd, Cr and Mn Concentrations Within Top-Soil of An Automobile Workshop Within University College Hospital, Ibadan, Nigeria" International Journal of Research and Innovation in Appli

International Journal of Research and Innovation in Applied Science (IJRIAS)

Utilization of AAS and GIS Tools in Assessment of Distribution of Pb, Cd, Cr and Mn Concentrations Within Top-Soil of An Automobile Workshop Within University College Hospital, Ibadan, Nigeria AFOLAYAN Adedotun Onoyinka*1, 2, POPOOLA Shola Caleb1 1National Open University of Nigeria (Ibadan Study Centre) 2Tissue Culture Unit, Biotechnology Department, National Centre for Genetic Resources and Biotechnology, P.M.B. 5382, Moor Plantation, Apata, Ibadan, Nigeria *Correspondence Author Abstract: -Increased frequency of acquisition and use of automobiles have required the establishment of repair workshop in different parts of most cityin developing countries. Apart from the vehicular emission, which is a major route of environmental pollution; the different waste materials generated in most of these auto-fixer workshops has contributed widely to environmental contamination and pollution with heavy metals. This study was designed and carried out to ascertain distribution and degree of pollution of selected heavy metals: Pb, Cd, Cr and Mn within an automobile workshop located at University college Hospital, Ibadan. The concentrations of Pb, Cd, Cr and Mn in top soil samples from this workshop were determined using Atomic Absorption Spectrophotometry (AAS). The mean concentrations of each of these heavy metals within the topsoil samples were 235.13mgPbkg-1, 5.28mgCdkg-1, 45.96mgCrkg-1 and 411.23mgMnkg-1 respectively and the abundance of the metals within the workshop’stop soil follows the order: Mn>>Pb>>Cr>Cd.Overall, the mean concentrations of Pb and Cd measured within the workshop’stop soil samples exceeded Standard Regulatory limits. ArcGIS software was employed in producing the geochemical maps. The Quantification of Contamination (QoC) analysis showed that Pb, Cd, Cr and Mn have anthropogenic source of contamination and the contamination factor values for Cd, Pb and Mnindicated values greater than 6. This study shows that the concentration and distribution of Pb, Cd, Cr and Mn within the study area soils correlates directly to activities originating from the automobile repair workshops. Consequently, these top soils overloaded with noxious metals will pose a health risk to the inhabiting populace of the area surrounding the workshop. Keywords: Automobile workshop, Contamination, Top Soil, Heavy metals, AAS, ArcGIS

View Article
March 2020
163 Reads

Isolation, Identification and Classification of Contaminating Microbes at an Ibadan-Based Plant Tissue Culture Laboratory

Adeye Joseph Ademola Oluwaferanmi, Afolayan Adedotun Onoyinka, Aladele Sunday Ezekiel , Jamaleddine Zainab Olubunmi. Isolation, Identification and Classification of Contaminating Microbes at an Ibadan-Based Plant Tissue Culture Laboratory. International Journal of Research and Innovation in Applied

International Journal of Research and Innovation in Applied Science (IJRIAS)

Abstract:-Microbial contamination in laboratories is a serious problem worldwide and characterization of these contaminants is imperative for achievement of successful in vitro research activities for immeasurable and unquantifiable agro-economic benefits. Literatures have shown that there is an ongoing effort(s) to identify the various groups of microbial contaminants within the plant cultures in mosttissue culture laboratories. Thus, this study has been designed to isolate, identify and classify the contaminating microbes at an Ibadan-based Plant Tissue Culture Laboratory. Established but contaminated cultures of different crop species were collected over a period of four weeks, from February 1st to 28 th , 2019. And from these, isolation of pure microbial cultures was carried out based on their morphological differences and where colony form, elevation, pigmentation and size were used to distinguish bacteria and fungi contaminants. Also, specific microbes were further authenticated using differential and selective media and isolated fungi were identified using microscopic observations of size, and shape. The results showed that the contaminating microbes are of different types. Themacroscopic and microscopic observations of fungi confirmed presence of Cladosporium sp, Penicillium sp, Aspergillus sp and Alternaria sp while the persistent bacteria identified were Shigella sp, Pseudomonasaeruginosa, Corynebacteria sp, Bacillus sp and Staphylococci aureus. The contaminants were similar to standard strains but there was a significant difference in contamination. It is concluded that despite disinfection with sodium hypochlorite, the bacterial and fungal contaminants persist in micropropagation culture media and there is need to either increase the concentration of the disinfectant or change the disinfectant to a different one. Keywords: micro-propagation, plant tissue culture, bacteria, fungi, contaminants.

View Article
January 2020
62 Reads

Concentration of Heavy Metals in Selected Commercial Dry Yam and Yam Powder Products Sold at Oja Oba and Bodija Market, Ibadan, Nigeria

ADEGOKE Adeola Florence1 , AFOLAYAN Adedotun Onoyinka2*. Concentration of Heavy Metals in Selected Commercial Dry Yam and Yam Powder Products Sold at Oja Oba and Bodija Market, Ibadan, Nigeria. International Journal of Research and Innovation In Applied Science (IJRIAS) | Volume V, Issue I, January

International Journal of Research and Innovation In Applied Science (IJRIAS)

Abstract— In vitro propagation is a plant tissue culture technique that provides a way to increase the rapid production of disease-free and non-contaminated planting materials, which boosts economic growth and support good health of the citizens. Being an aseptic procedure, it often helps to overcome constraints related with availability of both high-quality planting material and non-contaminated food production. The different yield of agronomic crops resulting from the contemporary agriculture practices, are often contaminated with pollutants such as heavy metals; especially during the course of their cultivation and occasionally during post-harvest processing procedures. Dioscorea sp. (Yam) is an important staple food in Nigeria with many health benefits but the presence of such noxious contaminants as heavy metals in its tubers and products renders its consumption highly detrimental to the health of its different consumers particularly human. This study was designed to conduct a comparative assessment of selected heavy metals concentrations in the dry tubers and powders originating from tissue culture hardened D. rotundata and D. alata with their respective market samples. Samples of tubers originating from yield of hardened in vitro propagated of D. rotundata and D. alata were collected from an Ibadan based Tissue Culture laboratory over a period of 28 days. Also, samples of the retailed dry tubers of both yam species were purchased concurrently from two renowned markets in Ibadan for comparison. These yam samples were all subjected to digestion and heavy metal analysis using Flame Atomic Absorption Spectrometer (AAS) to determine the levels of Pb, Cr, Cd and Ni in the digestates. The results obtained were also analyzed using XLSTAT analytical tool while pollution load indices and contamination factor were calculated. The results obtained showed that the levels of Pb, Cr, Cd and Ni in the dry tubers of in vitro yam samples (Cr=Ni=Pb=Cd; Cr = 0.000 mg/l) were significantly less than (p<0.005) those purchased from the two markets (Cr>Ni>Pb=Cd; Cr = 0.018± 0.025 - 0.030± 0.037 mg/l). Yam cultivation via micro-propagation and screen-house environments prevent undue exposure of the resulting tubers to heavy metal toxicity and as such reduces human exposure to heavy metal contaminated foodstuffs. Index Terms— In vitro propagation, Dioscorea rotundata, Dioscorea alata, Pollutants, Heavy metals, Tissue Culture, Toxicity

View Article
January 2020
60 Reads

Comparative Response of Five Varieties of Dioscorea Species (Yam) to Rapid MicroPropagation Using Single Node Vine Cuttings: A Measure Towards Income Generation, Food Security and Biodiversity Conservation in Nigeria

AFOLAYAN, Adedotun Onoyinka et al. 2018. Comparative Response of Five Varieties of Dioscorea Species (Yam) to Rapid MicroPropagation Using Single Node Vine Cuttings: A Measure Towards Income Generation, Food Security and Biodiversity Conservation in Nigeria. Proceedings of 6th NSCB Biodiversity Conf

Proceedings of 6th NSCB Biodiversity Conference; Uniuyo 2018

Abstract: Different micro-propagation techniques have been developed for yam varieties (Dioscorea sp.). Owing to the various challenges encountered in the process of regeneration and multiplication of the vegetative parts of yam in vitro, it becomes imperative to source for alternative but easily achievable and cheap method of micropropagation, hence the vine cutting experiment. Five certified disease-free varieties of Dioscorea sp. tagged by and collected from IITA/YIIFSWA Bio-Science Laboratory were utilised for the study. These are TDr 89/ 02665, TDa 98/01176, TDr 95/19177, Pona and TDr 95/18544. After acquisition, these were established in the screenhouse by planting them in potting bags using pasteurized topsoil. Successful growth was achieved by constant irrigation of the potted plants and the vines were supported with sterile twines for co-ordinated twirling. Ten (10) single node cuttings each were collected from each of the yam variety pots and sub-planted into smaller pasteurized topsoil pots. Quadruple replicates of these were done. The results indicated that while all the varieties’ nodal cuttings responded positively, the best response was gotten from TDa 98/01176, followed by TDr 89/ 02665, TDr 95/18544, TDr 95/19177 and Pona in that order. On the average, the fastest response in terms of days to emergence of new shoot and new aerial branches was obtained in TDr 98/01176 (11.375 ± 1.493 days and 31.250 ± 2.278 days respectively) while the slowest response was gotten from TDr 95/19177 (12.875 ± 1.436 days) for days to emergence of new shoot and Pona (43.750 ± 2.385 days) for days to emergence of new aerial branches. The average number of new aerial branches ranged from 2.250± 0.433 in Pona to 5.500± 1.118 in TDa 98/01176.Overall, the percentage survival follows the order Pona>TDr 89/02665>TDr 95/18544>(TDa 98/01176 = TDr 95/19177). Keywords: Micro-propagation, Yam, Topsoil, Vines, Node, Potting

View Article
May 2018
42 Reads

Spatial and temporal variations of physicochemical parameters in surface water of Wami River, Tanzania

Mkude, I.T., Kodom, K., Afolayan, A.O., Saria, J. and Mihale, M.J. (2018), “Spatial and temporal variations of physicochemical parameters in surface water of Wami River, Tanzania”, International Journal of Development and Sustainability, Vol. 7 No. 6, pp. 1936-1945.

International Journal of Development and Sustainability

Abstract The study analysed physicochemical parameters of the Wami river, in Tanzania to establish a diagnostic of surface water quality status of the river. Samplings were carried out on 15 points that regarded as upstream, mid-section and downstream during the dry season and rainy season. Results obtained show that almost all parameters analysed relatively good quality compared to international standards for fresh water. Temperature shows a slight drop changes in ranges seasonally from 27o C to 30o C during dry season to 24o C to 28o C during rainy season which is within the recommended range for aquatic life safety. A pH obtained are within the recommended range of 6.6 to 7.4 in both seasons. Furthermore, the progression of pH does not vary considerably between upstream and downstream in seasons. A rise in EC particularly in the dry season, 518 µScm-1 was observed compared to that of 208 µScm-1 in rainy season. The oxygen amount in water measured as DO and BOD exhibit a large spread of mean values seasonally and spatially with DO values of 0.02 ppm (downstream) to 9.80 ppm (upstream) both obtained on rainy season. BOD recorded values from 0.40ppm to 56.00ppm. Keywords: Water Quality; Physicochemical Parameters; Seasonal Variation; Wami River; Tanzania

View Article
May 2018
42 Reads

Accumulation of Heavy Metals from Battery Waste in Topsoil, Surface Water, and Garden Grown Maize at Omilende Area, Olodo, Nigeria.

Authors:
Afolayan A. O.

Afolayan AO. Accumulation of Heavy Metals from Battery Waste in Topsoil, Surface Water, and Garden Grown Maize at Omilende Area, Olodo, Nigeria. Global Challenges (Hoboken, NJ). 2018 Mar;2(3):1700090. DOI: 10.1002/gch2.201700090.

Global Challenges (Hoboken, NJ)

Full paper 1700090 (1 of 12) © 2018 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim www.global-challenges.com Accumulation of Heavy Metals from Battery Waste in Topsoil, Surface Water, and Garden Grown Maize at Omilende Area, Olodo, Nigeria Adedotun Onoyinka Afolayan Dr. A. O. Afolayan, Tissue Culture/Biotechnology Unit National Centre for Genetic Resources and Biotechnology P.M.B. 5382, Moor Plantation, Ibadan, Oyo State +234-02, Nigeria E-mail: onoyinka@yahoo.com DOI: 10.1002/gch2.201700090 Numerous industrial activities, including automobile battery production, have often resulted in the accumulation of noxious metals in the environment,[3–7] and the discharge of heavy metals as a by-product of these activities has been accompanied by largescale soil pollution.[7,8] Heavy metals tend to persist in the environment indefinitely[9] and get accumulated over time in soils, water sediments, and plants. Hence, they could have a negative influence on physiological activities of plants, determining the reductions in plant growth, dry matter accumulation, and yield.[10] Excessive accumulation of heavy metals in agricultural soils often leads to elevated heavy metal uptake by crops, and thus affects food quality and safety.[11] Food chain contamination is one of the important pathways for the entry of toxic pollutants into the human body,[12] and the consumption of heavy-metal-contaminated food can seriously deplete some essential nutrients in the body which are further responsible for decreasing immunological defenses, intrauterine growth retardation, impaired psychosocial faculties, disabilities associated with malnutrition, and high prevalence of upper gastrointestinal cancer rates.[13,14] Heavy metal accumulation in plants depends upon plant species, and the efficiency of different plants in absorbing metals is evaluated by either plant uptake or soil-to-plant transfer factors (TFs) of the metals.[15] Some of the heavy metals such as Pb and Cd are toxic to plants and animals, even in trace concentrations,[16] and exposure to some of them is normally chronic due to food chain transfer.[17] Pb and Cd have been found to inhibit plant growth, disturb ion uptake and transport, as well as inhibit enzyme activation and photosynthesis.[18,19] Fe is essential for many plant functions[20] but is toxic when it accumulates to high levels. Excess Fe can result in dark green foliage, stunted growth of tops and roots, dark brown to purple leaves in some plants.[20] Emissions of heavy metals to the environment occur via a wide range of processes and pathways, including air, surface water, and soil, and subsequently, ground water and crops.[21] Surface water can be polluted by contaminants that are washed into it from a nearby polluted site.[21] Remediation of soil contamination by conventional engineering techniques is often a very expensive procedure. This is Land pollution is a threat to sustainable agricultural development and food security in developing countries. Consumption of farm products from contaminated areas can generate health hazards to the diverse consumers along the food chain through the different pollutants in the products. This study is designed to determine the accumulation of Pb, Cd, and Fe in topsoil, surface water, and maize leaf, stem, grains, and root, cultivated in a garden nearby Ori-Ile battery waste dumpsite, Omilende Area, Olodo, Nigeria. Soil samples, garden maize parts, and surface water samples are collected from the study area using standard procedures. Corresponding reference samples are collected from Moor Plantation, Ibadan. All collected samples are analysed for Pb, Cd, and Fe concentrations. Mean Pb, Cd, and Fe concentrations in topsoil are found to be significantly higher than 157.0 ± 39.8, 2.2 ± 1.2, and 976.3 ± 353.9 mg kg−1, respectively, which are obtained from reference soil and National Environmental Standards and Regulations Enforcement Agency limits (Pb: 164 mg kg−1 and Cd: 50 mg kg−1). The soil contamination factor values obtained are greater than 6, indicating severe pollution. Downstream has the highest Pb, Cd, and Fe concentrations. In maize parts, the root has the highest concentration of Pb (40.95 ± 1.98 mg L−1) and Cd (2.84 ± 0.19 mg L−1), which are significantly higher (p ≤ 0.05) than those from the reference site. A high concentration of heavy metals found in topsoil further bio-accumulates in maize parts. Consequently, this garden maize is unfit for consumption. Heavy Metal Pollution © 2018 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. The ORCID identification number(s) for the author(s) of this article can be found under https://doi.org/10.1002/gch2.201700090.

View Article
February 2018
144 Reads

Physicochemical Parameters Assessment and Seasonal Variations of Surface Water Quality of the Volta Lake in Ghana

Kingsley Kodom, Afolayan Adedotun Onoyinka, Isabela Thomas Mkude, Solomon Ayeboafo Otu & Joy Okai Yeboah. 2018. Physicochemical Parameters Assessment and Seasonal Variations of Surface Water Quality of the Volta Lake in Ghana. International Journal of Research Available at https://edupediapublicatio

International Journal of Research

Abstract: Data for nine (9) physicochemical parameters (EC, SAL, ERes, Turb, pH, DO, TDS, Temp and % sat of DO) were investigated from 13 sampling locations of the Volta Lake, to assess the seasonal and spatial variations in surface water quality, using multivariate statistical techniques (PCA and EFA). The range of the assessed parameters were observed to have seasonal significant difference for EC (76.20 - 124.09 µS/cm), SAL (36.15 - 52.56 mg/L), ERes (10.0 - 16.44 KΩ.cm), Turb (0.44 - 13.98 NTU), pH (6.18-8.17), DO (3.22 - 6.90 mg/L), TDS (30.59 - 49.24 mg/L), Temp (23.6 - 32.9 °C) and % sat of DO (60.4 - 89.2 %), of which, pH, DO (% Sat), Temp and SAL were observed to be maximum, though marginal, in the Dry season. From the entire stretch of the lake, all the parameters recorded mean maximum values from the UVS, except for ERes (15.15 KΩ.cm) and Temp (31.8 °C), whose mean maximum values were respectfully recorded from the MVS and LVS. Conversely, the UVS recorded mean minimum values of pH (6.9), ERes (11.16 KΩ.cm) and Temp (26.6 °C) throughout the 2 seasons. But, that of SAL (37.7 mg/L), EC (82.91 µS/cm), TDS (33.18 mg/L) and Turb (4.91 NTU) were observed at the MVS (all in the Dry Season). For the LVS, DO (5.0 mg/L) and % sat of DO (74.3 %) were observed in the Dry season. The PCA/EFA showed both spatial and seasonal variations for the parameters at all the 13 sample locations. The seasonal correlations according to the PCA and EFA, slight changes were observed in the selection of the most significant parameters (EC, DO (% Sat), DO, SAL, TDS and ERes) responsible for seasonal variations of the Lake’s water quality based on a rotated factor correlation coefficient selection criterion. In view of this, seasonal variations of parameters must always be considered in water quality assessment when selecting parameters for environmental monitoring purposes in the reservoir because, parameters considered significant in contributing to water quality variations in a particular season may, or may not, be considered important in another season. Due to the unique biodiversity the reservoir supports, and the socioeconomic services it provides to thousands of surrounding communities, extensive monitoring network throughout seasons should be encouraged since changes in land-use practices and climate variability can significantly affect the reservoir. Keywords Physicochemical parameters; Multivariate statistics; Seasonal variations; Surface water quality; Reservoir

View Article
January 2018
43 Reads

Lead, Cadmium and Iron Concentrations in Zea Mays Grown Within the Vicinity of Ori-Ile Battery Waste Dumpsite, Olodo, Ibadan, Nigeria

Adedotun Onoyinka Afolayan, Amusat Titilayo Hassan. Lead, Cadmium and Iron Concentrations in Zea Mays Grown Within the Vicinity of Ori-Ile Battery Waste Dumpsite, Olodo, Ibadan, Nigeria. American Journal of Bioscience and Bioengineering. Vol. 5, No. 5, 2017, pp. 92-103. doi: 10.11648/j.bio.20170505.

American Journal of Bioscience and Bioengineering

Agricultural produce resulting from the cultivation of polluted soil can result in undue exposure and health hazards for its consumers at different level along the food chain. Available literature has shown that maize absorbs and accumulates toxic heavy metals from polluted soils. This study was designed to determine the concentration of lead (Pb), cadmium (Cd) and iron (Fe) within the leaf, stem, grains and root of maize grown on Ori-Ile battery waste dumpsite, Olodo, Ibadan Nigeria. Soil samples were collected every two months (March 2008 to July 2009) from the waste dumpsite and along the direction of the garden at 5m interval from the edge of the waste dump site. To determine the levels of uptake and accumulation of Pb, Cd, and Fe, maize was planted in a nearby house garden, situated within 25m distance away from the waste dumpsite and the root, stem, leaf and grains were harvested at maturity. All soil samples collected and the harvested maize parts were analysed for Pb, Cd, and Fe. Concentrations of Pb, Cd and Fe in soil were found to range from 3265.8±517.8 to 4351.3±1068.2; 163.96±23.2 to 258.38±123.1; 7712.90±473.8 to 8130.00±808.4 mg/kg respectively. These were significantly higher than values obtained from the reference soil (157.0±39.8, 2.2±1.2, 976.3±353.9 mg/kg respectively) and National Environmental Standard Regulation Agency (NESREA) limits (164mgPb/kg and 50mgCd/kg). Also, soil contamination factor values obtained were greater than 6 and this indicated severe contamination. Concentrations of Pb, Cd and Fe in maize-parts were significantly higher (P ≤ 0.05) than those cultivated on the reference site soil. Roots had the highest concentration of Pb (40.95±1.98 mg/L) and Cd (2.84±0.19 mg/L). In all maize-parts, bio-accumulation factor of the heavy metals was less than 1. Overall, these results have shown that the levels of metals in soil were several folds above the limits set by NESREA. Also, the range within the analysed plant parts was above the normal limit recommended for plants. High accumulation of heavy metals found in the soils of Ori-Ile battery waste dumpsite, Olodo, Ibadan bio-accumulated in maizeroots. Therefore, maize root is actively involved in phyto extraction of heavy metals from contaminated soils.

http://www.sciencepublishinggroup.com/j/bio

View Article
November 2017

112 Citations

85 Reads

Accumulation of Lead, Cadmium and Iron in Topsoil of Ori-Ile Battery Waste Dumpsite and Surrounding Gradient Point Areas at Olodo, Ibadan, Nigeria

Afolayan Adedotun Onoyinka, Hassan Amusat Titilayo. Accumulation of Lead, Cadmium and Iron in Topsoil of Ori-Ile Battery Waste Dumpsite and Surrounding Gradient Point Areas at Olodo, Ibadan, Nigeria. International Journal of Mineral Processing and Extractive Metallurgy. Vol. 2, No. 5, 2017, pp. 68-7

International Journal of Mineral Processing and Extractive Metallurgy

The disposal of waste is proving to be a major public health issue and a vital factor affecting the quality of the environment especially in the developing countries. Battery waste consists of toxic heavy metals and its incongruous disposal on the Ori-Ile battery waste dumpsite at Olodo, Ibadan has elicited public health concerns. This study was designed to determine the concentration of lead (Pb), cadmium (Cd) and iron (Fe) within the topsoil of Ori-Ile battery waste dumpsite and surrounding gradient point areas at Olodo, Ibadan, Nigeria. An auto-battery Waste Dumpsite (WD), Ori-Ile, Olodo was purposively selected for the study. One hundred and thirty six (136) soil samples were collected every two months (March 2008 to July 2009) from the waste dumpsite and along the along North, South, East and West (N, S, E, W) directions at 5 m intervals from the edge of the wastedump site. Control soil samples were collected from Moor Plantation (MP), Ibadan. All soil samples collected were analyzed for Pb, Cd, and Fe. Mean concentrations of Pb, Cd and Fe concentration (mg/kg) in topsoil from the waste dumpsite was Pb: 4273.8±1436.7, Cd: 258.4±123.1, Fe: 7910.0±791.5 while that from North was Pb: 4693.8±1107.9, Cd: 274.3±94.8, Fe: 8346.7±740.0; South was Pb: 4353.3±867.0, Cd: 255.2±71.4, Fe: 8189.6± 603.5; East was Pb: 4351.3±832.9, Cd: 248.2±65.6, Fe: 8130.0±639.5; West was Pb: 4698.3±1020.8, Cd: 278.4±86.9, Fe: 7851.3±676.8 respectively. These were significantly higher than values obtained from the reference soil (157.0±39.8, 2.2±1.2, 976.3±353.9 mg/kg respectively) and National Environmental Standard Regulation Agency (NESREA) limits (164mgPb/kg and 50mgCd/kg). Also, soil contamination factor values obtained were greater than 6 and this indicated severe contamination. Overall, these results have shown that the levels of Pb, Cd and Fe in all sampled topsoil were several folds above the limits set by NESREA. High accumulation of heavy metals was found in the topsoil of Ori-Ile battery waste dumpsite, Olodo, Ibadan and its surroundings and this could lead to further contamination of surface water, ground water and living organisms within this polluted sites. The health of all living biota within the area could also be negatively impacted.

http://www.sciencepublishinggroup.com/j/ijmpem

View Article
October 2017
55 Reads

Sublethal Effects of Methanolic Extract of Raphia hookeri on the Reproductive Capacity of Clarias gariepinus

Adedotun O. Afolayan, Temitope I. Borokini, and Gloria O. Afolayan. Sublethal Effects of Methanolic Extract of Raphia hookeri on the Reproductive Capacity of Clarias gariepinus. Hindawi Publishing Corporation Advances in Zoology Volume 2014, Article ID 615908, 10 pages http://dx.doi.org/10.1155/2014

Hindawi Publishing Corporation. Advances in Zoology

Raphia hookeri fruits are used for fishing in Nigeria due to their ichthyotoxic properties. This study investigated the toxic effects of R. hookeri on the reproductive capacity of Clarias gariepinus. The results from both short-term (96-hour test) and long-term (3-month sublethal test) bioassays revealed a linear relationship between R. hookeri extract dose and negative effects on the catfish. The percentage survival of both sexes of the catfish decreased with increasing extract concentration at short-term exposure, with LC50 values of 600 mg/L and 800 mg/L for male and female, respectively. At long-term exposure, the reproductive capacity of 10–12- month-old male and female brood-stocks diminished at relatively higher concentrations of R. hookeri fruit extract, with the gravid females producing fewer and mostly unviable eggs. The fruit extract also affected the eggs’ hatchability and fry survival when the exposed gravid females were treated with pituitary hormone and sperms from unexposed males, while the exposed males were unable to sexually stimulate female brooders. Sperms and pituitary hormone from exposed males were infertile, leading to low percentage of hatched eggs and mortality of the few hatched fries within 24 hours. These results confirmed the ethnobotanical use of this fruit extract for fishing in Nigeria.

View Article
November 2014
48 Reads