Dr. Abu-bakr M Gomaa, PhD - King Abdulaziz University & National Research Centre - Professor

Dr. Abu-bakr M Gomaa

PhD

King Abdulaziz University & National Research Centre

Professor

Jeddah & Cairo | Egypt

Main Specialties: Biology, Other

Additional Specialties: Microbiology and Microbial Ecology

ORCID logohttps://orcid.org/0000-0002-7035-5494


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Dr. Abu-bakr M Gomaa, PhD - King Abdulaziz University & National Research Centre - Professor

Dr. Abu-bakr M Gomaa

PhD

Introduction

Dr. Abu-Bakr M. Gomaa is professor of Microbiology and Microbial Ecology in both faculty of Science, King Abdulaziz University, Kingdom of Saudi Arabia and National Research Centre, Cairo Egypt. My fine specialties are Bioremediation, Biofertilization, Bio-organic Farming, Halophilic Microorganisms Application.

Primary Affiliation: King Abdulaziz University & National Research Centre - Jeddah & Cairo , Egypt

Specialties:

Additional Specialties:


View Dr. Abu-bakr M Gomaa’s Resume / CV

Education

Jan 2006 - Jul 2001
National Research Centre
Research Professor
Agricultural Microbiology
Jan 1995 - Jan 1995
Cairo University
Ph.D.
Agricultural Microbiology

Experience

Jun 2016
King Abdulaziz University, KSA
Professor
Biochemistry Department
Jan 2009 - Mar 2009
King Abdulaziz University, KSA
Professor
Biological Sciences Department

Publications

80Publications

85Reads

23Profile Views

9PubMed Central Citations

Significant antibacterial activity and synergistic effects of camel lactoferrin with antibiotics against methicillin-resistant Staphylococcus aureus (MRSA).

Res Microbiol 2016 Jul-Aug;167(6):480-91. Epub 2016 Apr 26.

Biological Sciences Department, Faculty of Science, King Abdulaziz University, P.O. Box 80203, Jeddah 21589, Saudi Arabia; Department of Molecular Medicine, USF Health Byrd Alzheimer's Research Institute, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USA; Laboratory of Structural Dynamics, Stability and Folding of Proteins, Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia.

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http://dx.doi.org/10.1016/j.resmic.2016.04.006DOI Listing
March 2017
50 Reads
2 Citations
2.705 Impact Factor

POINT PREVALENCE OF NOSOCOMIAL INFECTION IN THE THREE MAIN PUBLIC HOSPITALS IN JORDAN

Review Of Research

This descriptive study was carried out within the biggest three public hospitals in Kingdome of Jordan, i.e. Bashir, Prince Hamzah hospitals in Amman and Zarqa hospital. The obtained data were collected from the infection control committees in each hospital. The study is focusing on both patients and health workers in the concerned hospitals, the data were collected from the departments/words of surgical and wounds, acute surgical and orthopedic, urinary tract infections and ENT, ICU, CCU, internal medicine, catheterization lab and chemotherapy in the period from May 1st to May 30th. The prevalence and exposure-associated rates of Infection is reported according to age and sex including the occupational breakdowns in Jordan. the results show that three members of ESKAPE group; Pseudomonas aeruginosa, Klebsiella pneumonia in addition to Acinetobacter baumannii are the main causes of nosocomial acquired infections in the public hospitals in Jordan and the ICUs departments are the main infected departments distributed; device associated infections at participating ICUs; ventilator associated pneumonia (VAP), central line associated blood stream infection (CLABSI), catheter associated urinary tract infection (CAUTI), word acquired infections, community acquired infections and surgical site infection.

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December 2016
5 Reads

Isolation and Impact of Cellulose-Degrading Bacteria on Physico-chemical and Microbiological Properties of Plant Residues during the Aerobic Decomposition

Int. J. Curr. Microbiol. App. Sci

Five cellulose-decomposing Bacillus strains were isolated and the highly efficient two strains that were putatively identified as Bacillus megaterium and Bacillus brevis were used in the composting process. Plant residues were collected from the mown grass of King Abdulaziz University and composted for 60 days under aerobic conditions. Samples from the composting material were taken at 0, 12, 14, 33, 50 and 60 days and monitored for physical, chemical and microbiological analysis. The temperature of the compost pile rose to 44.0 ºC after one day of composting and reached its peak of 55.0 ºC after four days and lasted above 45.0 ºC until 30 days. The pH value of the residues decreased during the first 14 days of the composting process to reach 5.0 and thereafter it increased to reach 8.6 at the end of this process. The organic matter and carbon content of plant residues gradually decreased during the composting process and the biodegradability coefficient of the composting process was 0.5. The C:N ratio was narrowed rapidly to reach 14.0 in the produced compost. The N, P and K contents of the residues increased with increasing the composting period to reach 3.0, 0.73 and 2.4%, respectively, in the resultant compost. Moreover, the number of total microbe count, cellulose-decomposing microorganisms, fungi and actinomycetes gradually decreased within the first 14 days of the composting process and then, they increased after 33 days except the actinomycetes, that continued to decrease up to 50 days of composting.

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October 2016
6 Reads

APPLICATION OF EXTREME HALOPHILIC BACTERIA TO MITIGATE SALINITY STRESS ON TOMATO PLANTS GROWTH AND NUTRIENTS CONTENT AT FLOWERING STAGE

Indian Streams Research Journal

This investigation was implemented to study the impact of extreme halophilic bacteria on tomato plants grown under salinity stress. Nine different isolates were obtained from four Saudi Arabia and identified, i.e. 2 strains from Rabigh(Actinopolyspora xinjiangensis strain YIM 90829 and Phialosimplex chlamydosporus strain UAMH 10961), 3 strains from Al-Taif (Hamigera avellanea isolate NRRL 58017, Hamigera insecticola isolate NRRL 35442 and Hamigera insecticola strain AUMC 5731), 2 strains from Al-Madinah (Bacillus halochares strain JC225, isolate H-20C , Phialosimplex chlamydosporus strain UAMH 10961) and 2 strains from Makkah (Virgibacillus salarius strain SA-Vb1 and Halobacillus dabanensis isolate HD-02). These strains were obtained from the rhizosphere of acacia tree, cucumber, tomato and lemon tree respectively. The nitrogen content of the different strains ranged from 8.11 to 9.67 % against 6.77 % for the control. The highest value of nitrogen was found with Hamigera avellanea isolate NRRL 58017 isolated from Al-Taif governorate from the rhizosphere of cucumber and the lowest with Actinopolyspora xinjiangensis strain YIM 90829 isolated from Rabigh from the rhizosphere of acacia tree.It was found that the growth parameters of tomato plants (plant height and fresh & dry weights) inversely correlated with increasing salinity levels. The obtained result show that the different salinity levels (1000, 2500, 5000, 7500 and 10000 ppm) significantly decreased tomato plants growth parameters. It is worthy to mention that tomato seedlings exposed to the highest salinity level (10000 ppm) failed to continue growing up to flowering stage.Inoculation of tomato plants with the extreme halophilic bacteria either Bacillus halochares or Halobacilus dabanensis significantly increased plant height in comparison with the uninoculated control. For plant fresh weight, B. halochares did significant increase in comparison with either the control or Halobacillus dabanensis. No significant differences were recorded for plant dry weight. Regarding the influence of various salinity levels on tomato plants nutrient content, both salinity concentrations 2500 and 5000 ppm recorded the highest nitrogen content (2.29 and 2.28% respectively) of tomato plants that significantly overcame the other salinity levels. The high salinity level 7500ppm recorded the lowest nitrogen content. No significant variations were recorded among the various salinity levels expect that recorded with 7500 ppm where it lowered significantly the P-content in comparison with the other salinity levels. As to the potassium content of tomato plants, it was observed that potassium content was increased with increasing salinity level from 1000 to 5000 ppm and then decreased at 7500 ppm salinity level. Regarding tomato plants nitrogen content, it ranged from 1.31 to 1.79 % being the highest with the treatment of halobacillus dabanensis that significantly surpassed the control and the lowest with the treatment of Bacillus halochares that differed insignificantly with the control. For phosphorus content of tomato plants, insignificant differences were found among the various treatments. Application of the extreme halophilic bacteria did increase potassium content of tomato plants in comparison with the control. Despite the insignificant variation between both extreme halophilic strains, Bacillus halochares surpassed Halobacillus dabanensis, the percentage of increase reached 10.13.

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August 2016
5 Reads

BIO-REMEDIATION OF CD-CONTAMINATED SOIL CULTIVATED WITH FABA BEAN VIA APPLICATION OF ALCALIGENES FAECALIS RHIZOBACTERIUMR

Review Of Research

The present investigation was implemented to search for high efficient bacterial strains in bio-remediating the toxic influence of cadmium sulphate (CdSO.8HO) on faba bean plants grown in sandy soil supplemented with various concentrations of CdSO.8HO. Two isolates of Cd-remediating 42 bacterial strains were obtained from Al-Taif and Al-Madinah Al-Munawrah governorates, KSA from the rhizosphere of cucumber and tomato plants respectively. Regarding efficiency of both isolated strains in remediating CdSO4.8H2O supplemented nutrient broth medium. The absorbed quantities of Cd were 4295.66 and 84.00 ppm consecutively from 277 ppm Cd measured in the control treatment at the rate of 34.53% and 30.33% consecutively. The isolated bacterial strains were genetically identified as Alcaligenes faecalis strain BAB-1832 and Proteus mirabilis strain HI 4320. The bio-remediating strains Alcaligenes faecalis strain BAB-1832 revealed the highest efficiency in absorbing Cd that reached 95.66 ppm from 277ppm in the control. Application of A. faecalis significantly increased plant height of faba bean grown in soil containing 5000 ppm CdSO4.8HO, the percentage of increase over the same un-inoculated treatment reached 67.94. Bioremediation of contaminated soil with A. faecalis increased faba bean fresh weight at 5000 and 7500 ppm CdSO4.8H2O concentrations by 58.18 and 56.53% over the same not bio-remediated treatments. The bio-treatments of A.faecalis augmented faba bean P-content at various concentrations of CdSO4.8H2O (5000, 7500 and 10000 ppm). The Bio-remediated treatments of polluted soil with CdSO4.8H2O reduced faba bean plant Cd-content in comparison with their un-inoculated treatments. For the nitrogen content of faba bean plants, the differences recorded between the various treatments were not significant.

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March 2016
8 Reads

Status of the Biofertilization Technology andBiosafety of Biofertilizers in Egypt

B. Pati and S. M. Mandal (eds) Recent Trends in Biofertilizers, Chapter 2, pp. 19-36. I. K. Internat

Recent Trends in Biofertilizers, Chapter 2, pp. 19-36.

Biofertilization was taken just as a tool to reduce fertilizers input while other benefits were not considered properly, leading to a situation in which less educated/informed farmers were mostly unaware about biofertilization especially when chemical fertilizers are available in the market. In Egypt, biofertilization started more than 50 years ago by legume seed inoculation with rhizobia and rice fields with cyanobacteria, and later with Azospirilla for different crops. Recent emerging issues include stimulation of cereals performance by certain strains of cereal root inhabitants belonging to Rhizobium sp. Constraints facing the technology involve: (1) lack of fixed data describing the magnitude of adverse effects of excessive application of chemical fertilizers and pesticides on man, animal, plant, environment and national economy; (2) unsatisfactory information about potential of indigenous candidates; (3) most of the agronomic studies for evaluating the biofertilization lay in a “black box” category of research, in which, the positive results are deduced from indirect evidence; (4) unsatisfactory extension programmes and inadequate training of both the farmers and the extension experts; (5) conflicts between microbiologists and agronomists about what is better, synthetic fertilizers or biofertilizers; and, most importantly; (6) less adoption of tests for verification of the biosafety of biofertilizers before released. The latter is given special attention in this context. Future high priority topics may involve: (1) necessity of testing biosafety before release of biofertilizer preparations; (2) development of rapid assays that can predict performance of formulations in field level; (3) evaluation of compounds that promote cell survival on inoculated seed; (4) determination of effects of amendments, chemical fertilizers and pesticides; (5) development of new extension strategies and initiating innovate informative methods statistically sound in design; and (6) adoption of molecular biology methods to identify desirable characteristics, manipulates and introduces more efficient strains for specific inoculation purposes, (7) explore new associations and capitalize on their diversity, PGP activities, ecology (survival, colonization, endophytic state, biogeography, dispersal, etc., and 8) strengthen the electronic connections between researchers and extension experts working on informatics concerning active, effective and modern research and extension service programmes.

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February 2016
8 Reads

Saccharification and hydrolytic enzyme production of alkali pre-treated wheat bran by Trichoderma virens under solid state fermentation.

BMC Biotechnol 2015 May 28;15:37. Epub 2015 May 28.

Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.

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http://dx.doi.org/10.1186/s12896-015-0158-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4445277PMC
May 2015
19 Reads
1 Citation
2.034 Impact Factor

INFLUENCE OF SOME ANTIBIOTIC-PRODUCING ACTINOMYCETES ISOLATED FROM ECOLOGICALLY DIFFERENT AREAS IN SAUDI ARABIA ON THE HUMAN PATHOGENIC STAPHYLOCOCCUS AUREUS

Indian Streams Research Journal

This research was implemented to investigate the ecological distribution of antibiotic-producing actinomycetes in Saudi Arabia and their effect on the human pathogenic Staphylococcus aureus. A total of 124 actinomycetes isolates was isolated from the rhizosphere soil of some economic crops, i.e. date palm tree, tomato plants, beans plants, mango trees, squash plants and clover plants cultivated in ecologically different regionsin KSA (Northern, Southern, Central, Eastern and Western regions). The highest temperature was recorded in the West region at Makkah and Jeddah in the summer season that ranged from 35-50 oC and 39-47 oC consecutively. On the other hand, the lowest temperature was recorded in Tabuk at the North region that ranged from 0-15 oC in the winter season.Out of the 124 actinomycetes isolates, 15 isolates revealed antagonistic effect against the human pathogenic Staphylococcus aureus ATCC 25923. The 15 actinomycetes-producing antibiotics were identified using molecular technique of 16sr RNA.The ethyl acetate extract of the actinomycetes cultures supernatant recorded inhibition growth zones of Staphylococcus aureus (11.5-13.6 mm) more that those obtained with the cultures supernatant as such (8.0-11.7 mm). It was detected that, except Abha governorate in the South region, each region was inhabited by one species of actinomycetes despite the difference in the plant rhizosphere from which these species were isolated. Two strains of Streptomyces enissocaesilis were isolated for the rhizosphere of a date palm tree cultivated in Tabuk at the North region; while for the Middle Region (Al-Riyadh), five strains were isolated from the rhizosphere of three different plant (date palm tree, clover and tomato) and identified as Streptomyces sp. Two strains of Streptomyces fungicidicus were isolated from the rhizosphere of date palm tree and clover cultivated in Al-Dammam at the East region; while the three strains that isolated from the rhizosphere of a mango tree and beans plants were identified as Streptomyces ambofaciens. Regarding the South region (Abha), it was the only exception that found outside the context; where three different strains identified as Streptomyces maritimus, Streptomyces rochei and Actinobacterium sp. were isolated from the rhizosphere region date palm tree and beans plants. In general, this study reveals that the predominant ecological conditions in a region play an important role in the distribution of the antibiotic-producing actinomycetes.

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April 2015
5 Reads

The Bio-Organic Farming of Lettuce Crop and Its Impact on Macro-Nutrients of Plant and Soil

Wulfenia Journal

This study was conducted at the Agricultural Research Station, Hada Al-Sham, King Abdulaziz University, during the two successive seasons of 2014 to investigate the influence of bio-organic farming application vs. the recommended doses of mineral fertilizers (N, P, K) on N, P and K (%) in the leaves and roots of lettuce plant plus some soil properties. No significant differences were recorded for roots nutrient contents (N, P, K) due to the application of various treatments. Soil organic matter was significantly increased from 1.033% to 1.435% by using cellulose-decomposing bacteria. Soil pH was significantly decreased from 7.532 to 7.159 when the soil was treated with cellulose-decomposing bacteria. The combination of olive by-product + barley straw leaves waste water were the best organic fertilizers in improving plant N, P, K and soil organic matter, N, P, K content and pH.

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March 2015
3 Reads

BIOFERTILIZATION OF MAIZE GROWN UNDER SALINITY STRESS WITH AZOTOBACTER AND ITS IMPACT ON ROOT ANATOMICAL STRUCTURE

Review Of Research

A pot experiment was conducted in the greenhouse of the Faculty of Science, King Abdulaziz University on maize plants to study the impact of different salinity levels, i.e. 0, 1500 and 3000 ppm either in the presence absence of Azotobacter chroococcumon maize roots anatomical analysis. The mineral fertilization treatment with NPK (as a positive control) was resulted in smaller compacted and pressed cortical cells on the outer layers of cortical zone; however, the inner cortical zone was completely devoid of any traces of cortical cells. The area occupied by vascular tissue clearly revealed complete disruption of connective tissue. The interaction between NPK and salinity level of 1500 resulted in abnormal outline shape of root-cross section which tended to increase in length by 10% more than the treatment with NPK alone. Both salinity levels reduced stele diameters (length and width) with 4.76 and 23.81% respectively in comparison with the control treatment of mineral fertilization with NPK. The same trend was recorded with the number and thickness of metaxylem vessels where the reduction percent reached 47.05 and 3.38% consecutively with the treatment that received 3000 ppm salinity level against the NPK treatment. Inclusion of Azotobacter with both salinity levels resulted in normal shape of root cross-section. Such response also detected vascular tissue with 9 metaxylem vessels. Furthermore, biofertilization with Azotobacter combined with salinity level of 1500 ppm was resulted in more clear and healthy shape of root cross-section. Such effect was recognized in all tissue (cortical zone, pericycle, metaxylem vessels and connective tissue) of root-cross section. The outer and inner cortical layers were detected by 10 layers of cortical cells which were very clear in shape. The excellent shape, arrangement and photometric analysis were recognized in treatment of Azotobacter combined with salinity level of 1500 ppm. This positive influence of biofertilization with Azotobacter was attributed to its properties in biological nitrogen fixation and production of plant growth promoting substances.

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December 2014
3 Reads

NODULATION AND GROWTH PARAMETERS OF CASUARINA GROWN IN SANDY AND CALCAREOUS SOILS AS INFLUENCED BY LOCALLY ISOLATED FRANKIA STRAINS ISOLATED FROM ECOLOGICALLY DIFFERENT AREAS IN EGYPT

ROR. 2014 November 25; 4(2): 1-10.

Review Of Research

Sixteen Frankia pure isolates were isolated from three ecologically different Egyptian governorates i.e., five isolates from Kafr El-Sheikh, five isolates from New Valley and six isolates from Ismailia. According to their physiological properties, it was found that the isolated Frankia were identified as fifteen different Frankia strains belonging to the species of Frankia casuarina. In sandy soil, no nodules were formed in the control seedlings. The inoculated seedlings produced nodules dry weight ranged from 0.41 to 1.93 g/seedling with increases over the Br reference strain reached 13 to 15%. Inoculation of Casuarina seedlings with the local Frankia strains increased roots length and roots dry weight. The increases over the Br reference strain ranged from 3 to 130% and 0.4 to 139% consecutively. The isolated Frankia strains positively affect Casuarina stems length and number of branches in comparison with the Br reference strain. No significant differences were found regarding Casuarina shoots dry weight. For nodules dry weight, no nodules were formed in the control treatment. The isolated Frankia induced nodules dry weight ranged from 0.55 to 2.17 g/seedling, while the reference strain (Br) produced dry weight of nodules reached 1.20 g/seedling. For each of roots length and stems length, the isolated Frankia strains produced increases over the Br reference strain ranged from 2 to 104% and from 1 to 439% respectively. The dry weight of shoots of Casuarina seedlings grown in calcareous soil also positively affected by inoculation with the local Frankia strains in comparison with the Br reference strain, the percentage of increases ranged from 15 to 349. As for nitrogen content of roots and shoots of Casuarina seedlings grown in sandy soil, most of the locally isolated Frankia strains significantly overcame the Br reference strain. The increases in roots nitrogen content ranged from 17 to 137%, while for shoots they ranged from 10 to 123%. N-uptake in sandy soil was significantly affected by the majority of local Frankia strains; the increases reached 4% as a minimum and 354% as a maximum over the Br reference strain. In calcareous soil, the increases in nitrogen content of roots over the Br reference strain ranged from 21 to 225%. For shoots, these increases ranged between 14 and 131% over the Br reference strain. The isolated Frankia strains resulted in increases in N-uptake over the Br reference strain ranged between 20 and 395%. The majority of local Frankia strains were highly active where they acted well in both types of tested soils where the variations were not significant. The same trend was recorded with shoots nitrogen content due to the application of Frankia strains.

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November 2014
6 Reads

Solid fermentation of wheat bran for hydrolytic enzymes production and saccharification content by a local isolate Bacillus megatherium.

BMC Biotechnol 2014 Apr 24;14:29. Epub 2014 Apr 24.

Chemistry Department, Faculty of Science, King Abdulaziz University, P,O, Box 80203, Jeddah 21589, Saudi Arabia.

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http://dx.doi.org/10.1186/1472-6750-14-29DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4004419PMC
April 2014
16 Reads
6 Citations
2.034 Impact Factor

Seasonal Variation And Identification Of Diatoms In Al-nawras Bay In Saudi Arabia

Indian Streams Research Journal

This study was implemented during the period from spring 2008 to winter 2009 in Al-Nawras Bay located in Jeddah city, KSA to monitor the seasonal fluctuation of diatoms numbers as affected by the most predominant ecological factors in the bay. Identification of the prevalent diatoms species in the four study sites was achieved. Results showed that 24 diatoms species belonging to 12 genera were detected in the four tested sites. Of those, eight species were found in all four sites, while some species were found in one site but not in the others. For example, the species Navicula sp. 1, Navicula sp. 2, Nitzschia angularis, Nitzschia sp. 3 and Surirella fastuosa were found only in site A, which is the nearest site to a discharge point of mixed ground water in the Bay. Further, both diatoms species Amphora acutiuscula, and Surirella scalaris were found in site B that is located adjacent to site A. Meanwhile, site C contained the three species Amphora sp. 1, Amphora sp. 2 and Campylodiscus sp. The control site D, which is the farthest point from the pollution source, contained six diatom species (i.e., Tricertuim dubium, Amphora sp. 3, Bacteriastrum sp., Diploneis sp., Mastogloia sp. and Surirella hybrida). In terms of nutrient content and diatom numbers, site A recorded the highest number of diatoms and the highest content of nitrate, nitrite, ammonia, phosphate and silicate in comparison with the three other sites. The average number of diatoms in the four sites ranged from 2,343 to 19,188 cell/L with the fewest in site D and the highest in site A. Regarding seasonal variations of diatom numbers, the summer season significantly surpassed the other three seasons where the diatom numbers were 19,514, 9,158, 3,773 and 1,640 cell/L for summer, spring, autumn and winter, respectively. A positive, significant correlation was found between diatom numbers and transparency, nitrate, ammonia, sulphate and chlorophyll b, while a highly significant positive correlation was recorded between diatom numbers and chlorophyll a, chlorophyll c and carotenoids. In contrast, a negative correlation was detected between the diatom numbers and pH.

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October 2013
7 Reads

The Combined Effect of Mineral, Organic and Bio-Fertilizers on Growth of Some Wheat Cultivars

Journal of Applied Sciences Research

Two field experiments were conducted at the Experimental Station Farm of Faculty of Agriculture (Saba–Basha), Alexandria University, Egypt, during 2004/2005 and 2005/2006 growing seasons. The objective of this study was to investigate the effect of mineral, organic and bio-fertilizers on growth of two wheat cultivars to improve wheat growth and minimizing pollution. The results could be summarized as follows: Gemmiza 7 cultivar at the three mentioned stages of growth gave higher plant height, number of tillers/plant, dry weight/plant, leaf area/ plant , leaf area index, flag leaf area and specific leaf weight (75,96 and 117 days after sowing) in both seasons also, CGR and RGR at growth interval of 96-117 days after sowing. The addition of mineral and organic fertilizer at rate of 100% mineral fertilizer resulted in a significant increment in growth characters of wheat plants in both seasons at three growth stages and CGR at two growth intervals in both seasons. Significant variations were recorded between the tested biofertilizer treatments for growth characters at three growth stages and growth analysis at two growth intervals of wheat plants in both seasons. The effective treatments for plant height, leaf area / plant and leaf area index at 75,96 and 117 days after sowing and flag leaf area at 96 and 117 days after sowing were obtained from Gemmiza 7 cultivar with adding the full recommended dose of NPK (F5) in both seasons. The interaction between wheat cultivars and mineral, organic fertilizers was significant on NAR at two growth intervals in both seasons. The tallest plants, the largest leaf area, LAI and flag leaf area were obtained from planting Gemmiza 7 cultivar with yeast inoculation. The highest values of all growth characters ,NAR and CGR were obtained by using the full recommended NPK (F5) with inoculation wheat grains by yeast. Gemmiza 7 cultivar under using F5 with yeast inoculation gave the highest values of all studied growth characters, NAR and CGR.

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November 2011
5 Reads

Development of New Technological Approach to Mitigate Salinization

M. Behnassi et al. (eds.), Sustainable Agricultural Development, DOI 10.1007/978-94-007-0519-7_4, © Springer Science+Business Media B.V. 2011C

Salinization of land has threatened civilization from ancient to modern times. To mitigate salinization, efforts have been made in the aspects of management and reclamation including using salt tolerant crops. Soybean is one of the major food and oil crops in most of the countries where salinity problems exist or are likely to be developed. Reducing the spread of salinization and increasing the salt tolerance of high yielding crops are key global issues. In this regards, an experimental design was laid out during two successive summer seasons of 2007 and 2008 at three different sites with soil salinity levels of 3.13, 6.25 and 9.38 dS m−1 at the experimental farm of the Faculty of Agriculture, Fayum University, Egypt. The experiment was conducted to determine how can inoculation with Rhizobium japonicum, Azospirillum lipoferum and ascorbic acid solely and in combination mitigate the negative effects of salinity on soybean growth and yield. The experiment was conducted using randomized complete block design on three different sites with soil salinity levels of 3.13, 6.25 and 9.38 dS m−1, using two soybean cultivars (Giza22 and Giza111), 7 treatments (biofertilizer and ascorbic acid solely and their combinations) and three replications. The results revealed soil salinity significantly reduced plant height, number of leaves per plant, leaves area per plant (cm2), shoot dry weight, total chlorophyll and total caroteniods. Soil salinity significantly reduced ascorbic acid, total indoles, α-amylase activity and polyphenoloxidase activity while it increased total soluble phenols, total soluble sugars and free proline. Soil salinity decreased significantly the percentage of N, P, K, Fe, Mn, Zn and Cu while it increased Na and Cl in the plant. Soil salinity also reduced all seed yield parameters in addition to seed yield quality (protein and oil contents). No significant difference was found between both cultivars used in most measured traits. Biofertilizer treatment associated with ascorbic acid at 100 and 200 ppm showed the best results compared with other treatments (control, biofertilizer alone, ascorbic acid at 100 ppm and ascorbic acid at 200 ppm).

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February 2011
4 Reads

NODULATION, GROWTH PARAMETERS AND YIELD QUALITY OF FABA BEAN CULTIVATED IN A NEWLY RECLAIMED SANDY SOIL UNDER BIO-ORGANIC AGRICULTURE SYSTEM

INTERNATIONAL JOURNAL Of ACADEMIC RESEARCH

A field experiment was achieved during the two successive growing seasons of 2006-2007 and 2007-2008 in a newly cultivated sandy soil at El-Bostan region, Beheira Governorate, Egypt to investigate the application of bio-organic farming system and its influence on faba bean (Var. Giza 429) growth parameters, yield and yield quality. The obtained data indicated that application of either chicken or farmyard manure (FYM) accompanied by Rhizobium, Azotobacter and / or soil yeast (Rhodotorula sp.) induced increases for nodules number ranged from 22 % to 177 % over the positive control (100% NPK). The heaviest nodules weight was recorded due to the bio-organic treatment of chicken manure+Rhizobium+Azotobacter + Rhodotorula that reached 3.73 g/plant against 1.81 g/plant for the positive control. The percentage of increases for dry weight of plants (g/plant) ranged from 2 to 41% over the positive control owing to the different tested bio-organic treatments. The root / shoot (R/S) ratio percent ranged between 75.3 and 153.8% being the highest with FYM + Rhizobium+Azotobacter. The bio-organic treatments augmented the seed yield parameter (kg/fed.) in comparison with the positive control where the percentage of increases ranged from 12 to 39%. The bio-organic treatment of chicken manure + Rhizobium+Azotobacter+Rhodotorula induced the highest significant value of seed index when compared to the positive control. Except few cases, the majority of tested bio-organic treatments produced straw yield (kg/fed.) significantly overcame that obtained due to the application of positive control. No obvious differences were recorded between the tested bio-organic treatments and the positive control for nutrients content of faba bean seeds i.e., the percentages of nitrogen, protein %, phosphorus and potassium.

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September 2010
3 Reads

Bio-Organic Farming of Grain Sorghum and its Effect on Growth, Physiological andYield Parameters and Antioxidant Enzymes Activity

Research Journal of Agriculture and Biological Sciences

Two field experiments were conducted in the new land of Youssef El-Sedeek, Fayoum Governorate, Egypt during the two successive seasons of 2007 and 2008 to investigate the influence of certain bio-organic farming treatments on growth parameters, yield, chemical constituents and some enzyme activities of sorghum varieties i.e, Dorado and Giza 15. The obtained results indicated that sorghum cultivars Dorado and Giza 15 were significantly different for the studied growth parameters at40, 70 and 100 days old and yield and its components except the specific leaf area that was insignificant.Dorado c.v. surpassed Giza 15 cv. in dry weight/plant, LA, LAI, LAR, SLW, weight of panicle,grains panicle, shelling %, seed index, straw yield/plant, biological yield/plant, grain yield (ton/fed)., straw yield (ton/fed)., biological yield (ton/fed). and harvest index. There were significant differences of growth characters and yield and its components except specific leaf area and harvest index obtained when plant were applied by bio-fertilizer. the combined inoculation with Azotobacter vinelandlii and Pseudomonas fluorescens surpassed the single inoculation either in presence of full or double full dose of organic manure in all characters but the positive control of the recommended doses of NPK recorded the highest significant values in all growth characters at all tested plant ages and yield and its components, while the combined inoculation treatment in presence of either full or double full organic matter dose came in the second order. The effect of interaction between sorghum cultivars and bio-fertilizer showed a significant effect on all parameters under study except specific leaf weight, it is obvious from the results that Dorado cultivar fertilized with bio-fertilizer gave the highest results at most characters under study. In comparison with the positive control, comparable results, without significant differences, were obtained due to the application of diverse bio-organic treatments for phosphorus and potassium grain content. For nitrogen and protein grain content, the positive control recorded the highest values. As to the enzymes activities of peroxidase, and catalase, application of the double full dose of organic manure and the combined inoculum of Azotobacter and Pseudomonas increased both peroxidase and catalase irrespective of the tested sorghum varieties. Giza 15 sorghum variety showed high enzyme activities when compared to Dorado variety. The positive control recorded the lowest values of the activities of the tested enzymes.

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March 2010
7 Reads

ASSOCIATIVE IMPACT OF BIO- AND ORGANIC FERTILIZERS ON GERANIUM PLANTS GROWN UNDER SALINE CONDITIONS

INTERNATIONAL JOURNAL Of ACADEMIC RESEARCH

Salinization plays a major role in soil degradation. Every year more and more land becomes non productive owing to salt accumulation. So it seems valuable, to test medicinal plant for their salt tolerance capacity. In the present investigation, experiments were conducted to study the effect of saline water in addition to bio and organic fertilization treatment on geranium plant growth, mineral and chemical constituents. Salinity affected most of the morphological parameters and decreased the growth performance. The use of peanut compost slightly increased plant fresh and dry weights. Although the use of compost full dose at 3000 ppm salinity level led to an increase in plant height and number of branches in the second cut. For the chemical constituents, the biofertilizer and compost application under saline conditions did not show any positive change in N, P, K contents except for Na% where it reached 1.919 when using (half dose of compost + Bio) at 3000 ppm and reached 1.97 when using (full dose of peanut compost + Bio) at 6000 ppm compared to their control. The oil percentage decreased at high salinity level of 6000 ppm but at 3000 ppm the oil percentage reached 0.4 when treated with (HD+Bio) and 0.6% when plants were supplied with full dose of peanut compost compared to the control. The major essential oil constituents were Geraniol and Citronellol. The highest level of oxidized compounds was attained by the untreated plants (control) and the plants supplied with half dose of compost.

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September 2009
4 Reads

PCR-RFLP of 16s rRNA Amplification Techniques and Utilization of Different Carbon Sources Used for Identification of Frankia Spp. Isolated from Different Egyptian Governorates

Australian Journal of Basic and Applied Sciences

Symbiotic Frankia form specialized structures (nodules) along the root system of the host plants. The occurrence of Frankia inside root nodules providing the host plant with fixed nitrogen.The Molecular biology technique (PCR-RFLP) was used to detect the polymorphism of 16s rRNA in addition to the traditional physiological tests for the detection and distinction between the isolated Frankia strains. In this study, Frankia strains were isolated from ecologically different Egyptian governorates. According to their physiological properties i.e., use efficiency of different carbon sources e.g., sodium propionate, sodium pyruvate, sodium acetate, sucrose, glucose and mannitol in addition to the molecular biology technique, the Frankia isolates were identified.

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February 2009
4 Reads

The Associative Impact of Certain Diazotrophs and Farmyard Manure on Two Rice Varieties Grown in a Newly Cultivated Land

Research Journal of Agriculture and Biological Sciences

A field trial was implemented in a newly cultivated sand soil at Suez Governorate, Egypt during the two successive seasons of 2005/2006 and 2006/2007 to investigate the response of two different rice varieties (a salt sensitive Giza 177 cultivar and a salt tolerant Giza 178 cultivar) to bio-fertilization with the free living Azotobacter vinelandii and/or the endophytic Rhizobium leguminosarum in the presence of two levels of farmyard manure (10 and 20 m3/fed.) in addition to the half recommended doses of N, P and K mineral fertilizers. The obtained results indicated that the salt-tolerant Giza 178 rice variety significantly overcame the salt-sensitive Giza 177 variety for all tested growth and yield rice parameters.The application of full dose of farmyard manure (20m3/fed.) significantly surpassed the half dose of farmyard manure (10m3/fed.) for all tested characters. Both rice cultivars responded well to the combined inoculum of Azotobacter and Rhizobium than the single inoculation with each of them. The bio-fertilization treatments accompanied by half the recommended doses of N, P and K significantly overcame the positive control (100% NPK) for all tested parameters. The obtained results for bio-fertilization were attributed to the endophytic association of Rhizobium with rice roots and the N2- fixing capacity and growth hormonesproduction by Azotobacter.

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February 2009
4 Reads

Antagonistic Activities of Potato Associated Bacteria via Their Production of Hydrolytic Enzymes with Special Reference to Pectinases

Research Journal of Agriculture and Biological Sciences

Fifty two potato-associated bacteria were tested for their ability as biological control agents against the plant pathogens Phoma betae and Rhizoctonia solani. Forty six of tested bacterial strains have high antagonistic activities towards the two pathogens. The antagonistic mechanisms were examined in vitro via their production of hydrolytic enzymes. The study was focused on pectinolytic enzymes and their production, characterization and applications. Among the fifty two microbial isolates, Paenibacillus polymyxa showed the highest pectinolytic activities and the exo-polygalactouronase was the major in the culture filtrate. The maintenance of nutritional requirements such as inoculum size, incubation time and nitrogen sources were found to be highly significant factors on production of endo- and exo-polygalacturonase. The partially purified exo-polygalacturonase was active over a wide pH range (3.0 - 0.9). The optimal temperature of the enzyme indicated maximal activities at 60-70C which makes°it a good candidate for application in industrial processes. In addition it increased the amount of reducing sugar content of fruit pulps from 20 mg sugar per ml apple juice to 44mg and from 15mg sugar per banana juice to 62mg at 65C after 2hr .The dual roles of antagonistic activity of Paenibacillus polymyxa°against different plant pathogens make it appealing alternatives to hazardous fumigants and fungicides.

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May 2008
5 Reads

The Potential Impact of Biofertilization, Antioxidant and Micronutrients Application in Reducing Salinity Stress on Two Wheat Cultivars

Australian Journal of Basic and Applied Sciences

A field experiment was conducted for the two growing seasons of 2006 and 2007 at three soil sites of different salinity levels (4.72, 7.79 and 10.86 dSm-1). The experiment was designed to study the influence of salt affected soil in addition to years, micronutrients, antioxidant application and their interaction on two wheat cultivars (Sakha 93 and Sakha 94). Obtained results indicated that application of biofertilization generally raised the shoot fresh weight values in comparison with non-biofertilized ones, although treatments using micronutrients in addition to 100 & 200 ppm antioxidant improved plant fresh weight to reach those of the positive control (100% NPK). At high soil salinity levels a remarkable observation was found where the biofertilized treatments significantly overcame the non-biofertilized treatments and similar observation was estimated for plant shoot dry weight under the same previous mentioned conditions. Biofertilization with Candida tropicalis increased significantly the leaf area of tested wheat cultivars under and soil salinity levels. At high salinity level, it was found that treatments supplied by biofertilization with yeast mitigated the adverse effect of salinity. Application of yeast produced the highest spikes number m2 irrespective of salinity level and cultivar type, that ranged from 221 – 323, in comparison with the non-biofertilized ones (72 - 273). For grain yield m the same previous trend was recorded at lower salinity levels where biofertilized wheat yield ranged from 8 to 22 ardab/fed, while non- biofertilized plants ranged from 0.73 to 14.8 Ardab/fed.

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March 2008
4 Reads

Genetic Profile of Bacillus cereus and Bacillus subtilis Indigenous Isolates and Their Performance as Bio-control Agent Against the Plant Pathogen Fusarium oxysporum

Research Journal of Cell and Molecular Biology

Bacillus strains i.e., four Bacillus cereus and two Bacillus subtilis were isolated from soils of different governorates of Egypt, and tested for their ability to inhibit the plant fungal pathogens F.oxysporum by using three different media , nutrient agar (NA), King’s medium (Km) and King’s medium supplemented with FeCl3. All strains produced inhibition zones on Km medium while no effects were found when NA medium was used. Results indicated different systems of fungal antagonism among the tested strains. Each BcIs-6, BcSn-8 and BcNv-29 was found to produce siderophore as the antagonistic effect. While the other three strain were had one or more different systems for fungal antagonisms.The six Bacillus strains were described for their antibiotic resistance and plasmid content. Different antibiotic resistance and plasmid profiles were found. Plasmid numbers were ranged from one to five per tested strains. Only one plasmid with the same size was found among all B. cereus strains. The genetic relationships of the six Bacillus isolates were determined by a random amplified polymorphic DNA (RAPD)-polymerase chain reaction (PCR) method with seven random primers. The seven primers produced multiple band profiles with a number of amplified DNA fragments ranging from 1 to 11. Thesize and number of amplified fragments also varied with different primers which indicate random pattern of amplification. This pattern of amplification indicates a genetic heterogeneity between the studied bacterial isolates. The maximum number (53 fragments) was amplified with primer (B3) and the minimum number (20 fragments) was amplified with primer (A3). Therefore the markers used in the present investigation proved to be quite powerful in distinguishing different isolates. The identification of unique fragments for each isolate will help in rapid identification of the isolate and also can be further utilized to design a diagnostic marker which is isolate-specific. Such marker can be utilized for tracing the isolates and also to study their fitness in field. RAPD analysis was used to construct the parsimony tree depicting relationships among the six studied bacterial isolates. Data showed closer proximity between BcIs to BcLs (88.6%). Key words: Bio-control

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February 2008
4 Reads

Isolation, Characterization and Genetic Differentiation of Frankia sp. Isolated from Ecologically Different Egyptian Locations

Research Journal of Cell and Molecular Biology

A new sterilization method for Casuarina root nodules was adopted. Sixteen Frankia pure isolates were isolated from three ecologically different Egyptian governorates i.e., five isolates from Kafr El-Sheikh, five isolates from New Valley and six isolates from Ismailia. According to their physiological properties i.e., use efficiency of sodium propionate, sodium pyruvate, sodium acetate, sucrose, glucose and mannitol as different carbon sources; antibiotics growth sensitivity at 15, 30 and 60 µg ml-1 medium of Rifampicin, Kanamycin, Chloramphenicol, Spectinomycin, Tetracycline, Ampicillin, Erythromycin, Neomycin sulfate, Streptomycin sulfate and Vancomycin hydrochloride; salinity tolerance to 2.0, 4.0, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5, 9.0, 10.0 and 10.5 % of sodium chloride; plasmids number and RAPD PCR , it was found that the various obtained Frankia isolates were identified as sixteen different Frankia strains all belonging to Frankia Casuarina.

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January 2008
4 Reads

Seasonal Abundance and the Efficiency of Yeast Liquid Culture (Candida tropicalis) as Bait for Capturing the Oriental Wasps (Vespa orientalis L.) Under Egyptian Environment

Journal of Applied Sciences Research

Seasonal abundance and the efficiency of liquid culture of yeast (Candida tropicalis) as bait to capture the oriental wasps (Vespa orientalis L.), were studied at Dirut location, Assiut governorate during 2003 seasons. The oriental wasps started to appear in the first week of April and gradually decreased to the minimum levels during June (late spring) and July (earlier summer). Then, the activity of wasps increased gradually from the second week of August to the fourth week of September. Total numbers of wasps reached the highest values in October followed by September and November and then the number of oriental wasps decreased until disappeared at the fourth week of December. The modified traps recommended by Ministry of Agriculture were baited with 100% of 1.26×105 freshly prepared yeast liquid culture (Candida tropicalis)captured the highest mean numbers of oriental wasps after 24 hr. and 7 days during the active period of wasps(September, October and November). Using liquid yeast culture at 25% concentration recorded the lowest mean numbers of wasps captured in the traps.

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November 2006
4 Reads

The Integrated Fertilization of Thompson Seedless Grapevines with Organic Manures,Biofertilizers and Low Dose of Mineral Nitrogen

Research Journal of Agriculture and Biological Sciences

This investigation was planed to study the effect of minimizing the use of nitrogen fertilization in vine yards as 100% mineral N fertilization to only 50% mineral N and 50% organic i.e., (composted municipal solid waste or Farmyard manure) with or without added different sources of biofertilizer i.e., (Pseudomonas or yeast) on Thompson Seedless grapevines leaf mineral content, yield and fruit quality especially nitrate and nitrite in berries juice. The experiment was conducted through two successive seasons 2004 and 2005 on 12-year old, own-rooted Thompson Seedless grapevines grown on sandy soil in a private farm at Sadat district,Menoufia Government, Egypt. The vine spacing was 2.0 x 2.5 m (vine x row). Vines were cane pruned with three wire trellis, supported by (Telephone system) under drip irrigation system. Results indicated that N, P and K content in the leaves was not affected significantly when compare using 100% mineral N fertilizer(control) with other treatments using: 50 % mineral N fertilizer + 50% organic fertilizer with or without added biofertilizers. Yield and cluster weight improved due to application of 50% mineral N + 50% organic fertilizer as a Farmyard manure source with biofertilizers. Fruit quality was improved in terms of berry weight and berry size and followed the same trend of yield and cluster weight while TSS and acidity percentages and juice N and P contents were slightly affected. Nitrate and nitrite in berries juice were significantly reduced through using 50% mineral N fertilizer + 50% organic fertilizer compared with 100% mineral N fertilizer (control) more decrease in juice nitrate and nitrite was obtained by adding biofertilizers as a Pseudo source. Thus, it seem that fertilizing Thompson Seedless grapevine with 50% mineral N (250 g ammonium sulphate) + 50% organic fertilizer (5.6 kg) Farmyard manure + Pseudo biofertilizer (1 L) /vine is the promising treatment under sand soil conditions.

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June 2006
5 Reads

Organic Farming of Snap Bean and its Impact on Pests Population in Comparison with Certain Traditional Treatments

Research Journal of Agriculture and Biological Sciences

A field experiment was carried out during the two successive growing seasons of 2004 and 2005 at the experimental Station of National Research Centre to investigate the impact of organic fertilization with composted egg-plant, green pepper, maize and okra plant residues on bean infestation with whitefly, leafminers, aphids and red spiders as well as certain growth parameters and snap bean yield in comparison with the traditional recommended treatments. The obtained results show that the composted green pepper residue positively affects the reduction percent of infestation with the various tested blights. The reduction percent ranged from 15.8 for whitefly to 76.1 for red spiders; meanwhile the reduction percent reached 22.9 and 53.2 for leafminers and aphids consecutively. Organic fertilization with the composted residue of okra or farmyard manure reduced infestation numbers with three out of the four tested blights viz. whitefly, leafminers and red spiders for composted okra residue and leafminers, aphids and red spiders for farmyard manure. The reduction percent reached 29.6, 65.7 and 21.9 by turns for the composted okra residue and 55.0, 27.3 and 61.9 respectively for farmyard manure treatment. Leafminers were the only blight that affected by organic manuring with the composted egg-plant residue where the reduction percent amounted 33.6, while chicken manure application positively affected reduction percent of infestation with each of whitefly and leaf miners as the reduction percent reached 57.8 and 74.0 respectively. The positive control affect the infestation numbers of leaf miners, aphids and red spiders with reduction percent reached 60.0, 36.7 and 67.7 by turns. Comparable results to the positive control were obtained for the tested bean growth parameters owing to the organic manuring of bean plants with the diverse composted agricultural residues without significant variations. Organic manuring of bean plants with different types of composted agricultural residues induced yield of snap bean significantly overcame the positive control either alone or when accompanied with micro elements.

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June 2006
4 Reads

Performance of Prickly Oil Lettuce Biofertilized with Pseudomonas under Two Levels of Both Nitrogen Fertilization and Plant Density

Journal of Applied Sciences Research

A field trial was implemented during the two growing successive seasons of 2002/2003 and 2003/2004 at the Agricultural Experimental Station of National Research Centre to look for an integrated fertilization regime suitable for prickly oil lettuce. The proposed scheme of work is comprising of biofertilization with Pseudomonas fluorescence, two levels of nitrogen fertilization and/or two rates of plant density. Both branches number and length of seeds area was not affected by the plant density per feddan, while significant differences in favor of the low plant density were recorded for plant height, seed yield per plant straw yield, biological yield and seed index. The high plant density significantly overcame the low one for seed yield per feddan. Biofertilization induced matchable results to the nitrogen fertilization for the majority of test growth and yield parameters. The comparison was in favor of the application of 40 unit of nitrogen per feddan than 20 unit. The treatment of high plant density biofertilized with Pseudomonas in the presence of 40 unit of nitrogen recorded the highest significant values of each of straw yield, biological yield and seed index, while the increase in seed yield (kg/fed.) was not significant in comparison with the other treatments. Inclusion of Pseudomonas to the various treatments increased oil yield (kg/fed.) and reduced the peroxide value. The oil content of prickly oil lettuce ranged from 39.80 to 41.42 % being the highest with the biofertilized treatment.The total saturated fatty acids ranged from 9.6 % to 12.0 % while the unsaturated fatty acids ranged between 87.69 % and 90.40% irrespective of the applied treatments.

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June 2006
4 Reads

Performance of Thompson Seedless Grapevine as Influenced by Organic Fertilizer, Humic Acid and Biofertilizers under Sandy Soil Conditions

Research Journal of Agriculture and Biological Sciences

The aim of this investigation is to evaluate the replacement of mineral nitrogen fertilization through using organic source [composted municipal solid waste (MSW)] and humic acid (HA) at 0.5, 1 and 2% with or without biofertilizers [Pseudomonas fluorescens + yeast (Saccharomyces cerevisae)] on leaf mineral content, yield, fruit quality and the residual NO3- and NO2- in berry juice of Thompson seedless grapevines. The study was carried out during two successive seasons (2004 and 2005) on 12 years old Thompson seedless grape vine planted on sandy soil under drip irrigation system in a private farm located at El-Sadat district, Minufiya governorate, Egypt. Results indicated that (MSW) fertilization reduced leaf mineral N and K compared with100% mineral N fertilization, while P content was not affected. Applying HA with MSW increased yield significantly than those fertilized with MSW alone. However, adding biofertilizer with humic slightly and not significantly increased yield than without adding it. On the other hand, results did not show a constant trend due to different treatments in respect with cluster weight and berry weight TSS was not affected, while acidity was decreased only in the second season by different treatments than 100% mineral N (control). Fertilizing vines with MSW alone or with HA significantly decreased nitrogen, nitrate and nitrite content in berries juice than those of mineral N fertilizer (control). Finally, it seems that fertilizing Thompson seedless grapevines with composted municipal solid waste (MSW) + 0.5% humic acid (HA) with or without biofertilizer are the promising treatments under this study conditions.

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June 2006
3 Reads

Application of Yeast Culture (Candida tropicalis) as Pollen Substitute in Feeding Honey BeeColonies (Apis mellifera L.) In Egypt

Journal of Applied Sciences Research

Artificial diets of a pure liquid culture of yeast in different concentrations as pollen grains substitute were used to feed honey bee colonies and compared with traditional artificial diets, Pollen grains, single dose of Brewer’s yeast, double dose of Brewer’s yeast and pollen substitute of soybean. During the experimental period some biological activities, areas of sealed worker and drone broods, mean number of combs covered with bees, mean number of queen cups, mean number of package bees and honey yield of the tested colonies were determined. The obtained results showed that application of 25% and 50% of yeast culture resulted the highest significant amount of worker brood in comparison with other treatments during the period of October, 2003 to June, 2004. The highest significant amount of sealed drone brood was recorded in March and April, 2004 due to the application of 25% of yeast culture. Colonies fed with 25% of the yeast culture recorded significantly mean number of combs covered with bees higher than the control and the recommended traditional honey bee artificial diets. Producing the queen cups increased with fed colonies by 50% of yeast culture overmatched either the control or the other tested treatments followed by 25% of yeast culture especially in April, May and June. Application of yeast culture in 25% or 50% induced the highest productivity of honey yield and numbers of package bees.

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May 2005
8 Reads

Interactive Effect of Rhizobium Inoculation, Sodium Benzoate and Salinity on Performance and Oxidative Stress in Two Faba bean Varieties

INTERNATIONAL JOURNAL OF AGRICULTURE & BIOLOGY

A pot trial was designed to investigate the impact of Rhizobium inoculation and/or sodium benzoate application on performance of two contrasting faba bean varieties i.e., Giza Blanka (salt tolerant) and Giza 634 (salt sensitive) grown in sandy soil under two levels of salinity (3000 and 6000 ppm). High salinity (6000 ppm) greatly reduced nodules formation in both varieties. Rhizobium inoculation reduced the inhibitory effect of salinity and plants were able to survive better. Giza Blanka, surpassed the other variety in all characteristics. Leaf area, Ka+ : Na+ ratio, protein content, malondialdehyde and superoxide dismutase activity were greatly affected by increasing salinity in both varieties. Hydroxyl scavenger, (sodium benzoate) positively improved leaf area reduced transpiration rate, malondialdehyde content (MDA) in plant leaves and showed a slight increase in the activity of enzyme superoxide dismutase (SOD). Rhizobium inoculation increased plant leaf area, reduced MDA content in plant leaves and did not show a significant effect on SOD activity in plant roots, but the interaction between Rhizobium inoculation and sodium benzoate resulted in a significant increase in SOD enzyme activity in plant roots.

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March 2005
3 Reads

Effect of organic and biofertilizers on the growth and yield of wheat plants

Egypt. J. Agric. Res., 2(2): 711-725.

Egyptian Journal of Agricultural Research

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February 2005
6 Reads

Effect of feeding biologically treated or non-treated grape pomace on growth performance of rabbits

The 4th International Conference on Rabbit Production in Hot Climates, Sharm El-Sheikh, Egypt, 275-283

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February 2005
6 Reads

Application of Bio-Organic Farming and its Influence on certain Pests Infestation,Growth and Productivity of Potato Plants

Journal of Applied Sciences Research

A pot trial was implemented in the greenhouse of National Research Centre, Cairo, Egypt to investigate the associative influence of certain composted agricultural residues i.e., egg-plant; okra, pepper and maize stalks and the foliar application of yeast culture (Candida tropicalis) on the infestation of potato plants with whitefly (Bemisia tabaci) and thrips (Thrips tabaci) as well as its effect on some growth and yield parameters of potato. Organic fertilization of potato plants with egg-plant compost significantly reduced the infestation numbers of potato plants either with whitefly or thrips. For whitefly infestation, the reduction percent reached 54 in comparison with the control and 24 when compared with the recommended doses of NPK treatment. Concerning thrips infestation, the reductions amounted to 80% in comparison with the control and 73% when compared to the recommended doses of NPK treatment. The reduction percentages in whitefly infestation numbers ranged from 21 to 54 due to the organic fertilization with either various types of composted agricultural residues or the traditional chicken manure and farm yard manure. These reduction percentages ranged from 11 to 80 for thrips infestation numbers. The foliar application of yeast reduced the infestation numbers of white fly and thrips in comparison with the corresponding untreated treatments with yeast. The reduction percentages ranged from 0.2 to 17.9 for whitefly and from 33 to 64 regarding thrips. A synergistic effect was recorded between organic fertilization and the foliar application of yeast on potato yield, where the combined application of organic fertilizers and yeast resulted in significant variations in comparison with the organic fertilizers as such. The increasing percentages in potato yield ranged from 21 to 73.

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February 2005
3 Reads

Recent Studies on the Rhizobium-Cereal Association

Current Plant Science and Biotechnology In Agriculture

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January 2005
4 Reads

Yield and essential oil quality of coriander as influenced by chicken manure, rock phosphate, sulfur and a phosphate solubilizing Bacillus treatments

J. Agric. Sci., Mansoura Univ., 29(9): 5231-5244.

Journal of Agricultural Sciences, Mansoura University

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September 2004
4 Reads

A comparative study between some synthetic growth hormones and certain growth hormones producing microorganisms on growth and essential oil composition of Salvia officinalis L. plants

Annals of Agric. Sci. Fac. Agric., Ain Shams Univ., Cairo, 49: 299-311

Annals of Agricultural Science, Ain Shams Univ., Cairo

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February 2004
5 Reads

Efficiency of applying biofertilizers to maize under different levels of chemical fertilizers

Annals of Agric. Sc., Moshtohor, 41(4): 1411-1420

Annals of Agricultural Science, Moshtohor

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April 2003
6 Reads

Growth parameters and oil yield quality of Genovese basil grown under organic farming system

Egypt. Pharm. J., 2: 150-169

Egyptian Pharmaceutical Journal

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February 2003
5 Reads

Genetic characterization and improvement of new local Bacillus thuringiensis isolates with elevated virulent to Spodoptera littoralis (Boisd.)

Journal of Genetic Engineering & Biotechnology (NRC), 1(1): 27-40

Journal of Genetic Engineering & Biotechnology (NRC)

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January 2003
5 Reads

Quantitative Indices for the Autecological Biogeography of a Rhizobium Endophyte of Rice at Macro and Micro Spatial Scales

Symbiosis, 35 (2003): 147-158

Symbiosis

Rhizobium leguminosarum bv. trifolii E1l is a natural root endophyte of rice isolated in the Nile delta where rice and berseem clover have been rotated since antiquity. Its autecological biogeography is being examined at two spatial scales:one at a macro scale relevant to its proposed use as a plant growth-promotive biofertilizer in rice-berseem clover agroecosystems, and a second at a micro scale relevant to its colonization of rice roots. Here we introduce two new indices to measure the prevalence in distribution of strain ElL within a defined spatial domain. An autecological biogeography index is described to map the distribution of a specific strain of rhizobia on a macro scale based on immunofluorescence microscopy of nodule occupants on legume trap hosts. A cluster index is introduced to analyze the in situ spatial pattern of root colonization by the bacteria at single-cell resolution using CMEIAS (Center for Microbial Ecology Image Analysis System) software for computer-assisted microscopy. When sampled at multiple georeferenced sites (i.e., at known Cartesian x, y coordinates relative to a landmark origin), these two indices provide values that are suitable for general use as the Z variate in spatial geostatistical analyses to model bacterial dispersion and colonization behavior, and to produce interpolated maps of the continuous distribution of the microbial symbiont within the defined spatial domain of the geographical region or root substratum, including areas that cannot be physically sampled. Both indices also have the potential for broad applications in microbial ecology.

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January 2003
3 Reads

The integrated system of Bio-and organic fertilizers for improving growth and yield of triticale

Egyptian J. Appl. Sci., 17(10): 512-523

Egyptian Journal of Applied Science

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October 2002
5 Reads

Growth traits and metabolic profile of New Zealand White rabbits fed on mash or pelleted diets supplemented with a yeast culture

Mansoura Univ. J. Agric. Sci., 27(6): 3737-3745

Mansoura University Journal of Agricultural Sciences

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June 2002
4 Reads

Suppressing growth of deteriorative fungi prevailing in historical carpets using certain chemical and biological materials

7th Scientific Conference on “The Art and City in the Third Millennium”, 30 Marsh-1 April. Fac. Fine Arts, Menia Univ., Egypt.

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March 2002
3 Reads

Evaluation of sugar filter cake as non-traditional foodstuffs in rabbits

2nd Int. Con. on Animal Prod. & Health in Semi Arid Area. 4-6 September, El-Arish, North Sinai, Egypt

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September 2001
5 Reads

Municipal solid waste (MSW) composting, practical aspects and product characterization

Egypt. J. Appl. Microbiol., 36(4): 461-475

Egyptian Journal of Applied Microbiology

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April 2001
4 Reads

RESPONSE OF A LOCAL AND SOME EXOTIC MUNGBEAN VARIETIES TO BIO- AND MINERAL FERTILIZATION

Acta Agronomica Hungarica, 49(3): 251–259 (2001)

Acta Agronomica Hungarica

A pot trial was carried out in the greenhouse of the National Research Centre during the summer season of 1999 to investigate the combined impact of Bradyrhizobium sp.(vigna) and Azotobacter vinelandii in the presence of various doses of chemical fertilizers, i.e. 25%, 50% and 100% of the recommended dose of NPK, on nodulation, growth parameters, seed yield and its components, and seed contents of protein, phosphorus and potassium of a local (Kawmy-1) and three exotic (VC-4, VC-9 and King) varieties of mungbean. The results indicated that there were no significant differences between the different varieties for nodule number per plant, while significant variations were obtained between both varieties and biofertilization treatments.The plants of the Kawmy-1 variety gave the shortest period of growth, the highest number of pods per plant and the highest values of harvest index and seed protein content.The King variety had the longest period of growth and the highest values for number of branches, seed yield, biological yield, seed index and seed phosphorus content. The inclusion of Azotobacter vinelandii significantly augmented various tested parameters,with the exception of seed yield, biological yield and potassium content, in comparison with Bradyrhizobium. Insignificant differences were found owing to the interaction between varieties, various levels of mineral fertilizers and types of biofertilizers with the exception of harvest index and seed phosphorus content.

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March 2001
4 Reads

Possibility of reducing chemical fertilization recommended for mungbean production using biofertilizers

Authors:
Gomaa A. M.

Annals of Agricultural Science, Moshtohor 38(3): 1363-1372

Annals of Agricultural Science, Moshtohor

A field trial (Egypt) was executed on mungbean in a clay soil for investigating to what extent biofertilizer application might replace chemical fertilizers recommended for getting the optimum growth and yield. The obtained results showed that Bradyrhizobium combined with Azospirillum and/or Candida in the presence of low levels of chemical fertilizers (0,30 or 60% of the recommended dose of NPK) ameliorated nodulation of mungbean. The highest significant increase was recorded due to the treatment of Bradyrhizobium + Candida + 30% NPK i.e., 76 nodules/plant and the lowest with Bradyrhizobium + Azospirillum + 60% NPK (58 nodules/plant) against 22 nodules/plant for the control (Bradyrhizobium + 100% NPK). Furthermore, significant differences were recorded in plant dry weight owing to the bio-treatment of Bradyrhizobium + Azospirillum + Candida in the presence of reduced doses of NPK. Seed yield/fed was also increased due to biofertilization where the percentage of increases over control ranged from 13 to 71. Insignificant differences were recorded for protein content (%) of plant, seed and shell. However, highly significant differences were obtained due to biofertilizer application for N2-uptake of plant, seed and shell.

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March 2000
5 Reads

The contingent interaction among specific free-living micro-residents and arbuscular mycorrhizal fungi under certain conditions

3ed Arab Conference on Modern Biotechnology and Areas of Application in the Arab World. Cairo, 14-17 December.

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December 1998
3 Reads

Associative action of a multistrain biofertilizer on tomato plants grown in a newly reclaimed sandy soil

The Sixth International Symposium on Nitrogen Fixation with Non-legumes, Ismailia-Egypt, 6-10 September 1993.

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September 1993
5 Reads

Enrichment of tomato sand cultures with composite inocula of associative dinitrogen fixers, P-dissolving bacilli and VAM.

The 5th International Symposium on Nitrogen Fixation With Non-Legumes, Florence, Italy, Kluwer Academic Publishers, Dordrecht, The Netherlands

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September 1990
5 Reads

The role of plasmids on Bacillus alkaline protease production in local isolated strains

Annals Agric. Sci., Ain Shams Univ., Cairo, 42(2): 573-584.

Annals of Agricultural Science, Ain Shams Univ., Cairo

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4 Reads

SYNERGISTIC EFFECT OF BIO-AND CHEMICAL FERTILIZERS TO IMPROVE QUALITY AND YIELD OF SNAP BEAN GROWN IN SANDY SOIL

XXV International Horticultural Congress, Part 3: Culture Techniques with Special Emphasis on Enviro

Acta Horticulturae

A field experiment was carried out in two successive autumn seasons (1996 and 1997) at El Bossily site on the west side of Roistta branch of the River Nile in Egypt to study the response of snap bean (Phaseolus vulgaris L.) var. Giza 3 to inoculation with bio-fertilizers i.e. non-symbiotic Azospirillum lipoferum and soil yeast (Candida sp.), in the presence of decreased doses of chemical fertilizers i.e 50% and 75% of NPK doses compared with 100% of NPK on vegetative growth parameters, pods quality and nodulation status. The results obtained showed that bio-fertilizers significantly enhanced nodules formation, especially in the presence of decreased doses of chemical fertilizers. Vegetative growth parameter i.e. plant height, leaves number, fresh and dry weight per plant were significantly increased by the combination of bio-fertilizers and/or different levels of NPK. Also, bio-fertilizers with various rates of NPK improved pods quality, such as increased crude protein percentage, increased total carbohydrate content and reduced the fiber percentage. It can be concluded that Giza 3 variety showed better vigorous growth plants with higher quality and yield of pods in response to bio-fertilizer applications with the two levels of NPK (50,75%) under sandy soil conditions.

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