Publications by authors named "Abolfazl Abbaszadeh"

22 Publications

  • Page 1 of 1

Isolated Congenital Absence of Right Lower Lateral Cartilage and Reconstruction with Septal Cartilage: A Case Report and Review of Literature.

World J Plast Surg 2021 May;10(2):120-123

School of Medicine, University of Central Lancashire, Preston, United Kingdom.

Congenital anomalies of the nose are rare but may be associated with syndromes affecting craniofacial structures. Herein, we report a case of a congenital absence of lower lateral cartilage seen during an open rhinoplasty in a 23-yr-old lady with no underlying health conditions. Medical and surgical history were unremarkable and there were no evidences conducted of any previous traumatic facial injuries. During physical examination, a significant nostril asymmetry was noted to be present. In addition, cotton test showed no evidence of obstruction. The absence of lower lateral cartilage on the right side was noted during the degloving stage of the open rhinoplasty. Absence of lower lateral cartilage poses a technical challenge in surgery and in order to reconstruct this structure, cartilage can be harvested from concha, lower lateral cartilage, septum and cartilaginous dorsal hump during an open approach rhinoplasty.
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http://dx.doi.org/10.29252/wjps.10.2.120DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290446PMC
May 2021

Neuroprotective and Anti-inflammatory Role of Atorvastatin and Its Interaction with Nitric Oxide (NO) in Chronic Constriction Injury-induced Neuropathic Pain.

Iran J Pharm Res 2020 ;19(4):67-75

Department of Clinical Pharmacy, Faculty of Pharmacy, Lorestan University of Medical Sciences, Khorramabad, Iran.

Prevention and treatment of neuropathic pain (NP) is one of the most difficult problems in clinical practice since the underlying mechanism of NP is unclear. In previous studies, the increased production of nitric oxide (NO) has been closely linked to the induced NP. In this study, we assessed the effect of atorvastatin through NO mechanism, on inflammation, thermal hyperalgesia, thermal allodynia, and mechanical allodynia as well as sciatic nerve histological score in rat with chronic constriction injury (CCI) model. Finally, we specified the role of cytokines such as TNF-α and IL-6 in the spinal cord. Treatment with atorvastatin and L-NAME (NO inhibitor) attenuated the thermal hyperalgesia, thermal allodynia and mechanical allodynia induced by CCI. The antinociceptive consequence was better elevated with a combination of atorvastatin and L-NAME in comparison with the other groups. In addition, the treatment with these drugs also attenuated the CCI-induced TNF-α and IL-6 level in the spinal cord. Furthermore, the histological analysis showed a low level of inflammation in the sciatic nerve in the CCI rats co-treated with atorvastatin and L-NAME. Findings of our study in NP-induced CCI in the rat model demonstrate that inhibition of NO displays antinociceptive and anti-neuroinflammatory effects of atorvastatin in peripheral and central nervous system. In addition, we found that inhibition of the NO by atorvastatin could be one of the most important anti-inflammatory pathways of atorvastatin effect.
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http://dx.doi.org/10.22037/ijpr.2020.1101230DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8019881PMC
January 2020

A Stereological Study of the Toxic Effects of Cerium Oxide during Pregnancy on Kidney Tissues in Neonatal NMRI Mice.

Oxid Med Cell Longev 2020 23;2020:9132724. Epub 2020 Jun 23.

Department of Anatomy, Kermanshah University of Medical Sciences, Kermanshah, Iran.

Background: Both antioxidant and prooxidant activities have been previously reported for cerium oxide (CeO). The aim of this study was to investigate the effects of CeO at different doses on changes in kidney tissues and markers in neonatal mice.

Methods: We randomly divided 30 pregnant NMRI mice into five groups ( = 6 per group)-a control group and four groups treated with intraperitoneal (i.p.) administration of different doses of CeO (10, 25, 80, or 250 mg/kg body weight (bw)) on gestation days (GD) 7 and GD14. At the end of the treatment period, we analyzed the kidney tissues and serum samples. The levels of two serum redox markers, malondialdehyde (MDA) and ferric reducing/antioxidant power (FRAP), were determined. Data were analyzed using one-way ANOVA and Tukey's test, and a value of <0.05 was considered significant.

Results: The mean total volumes of the renal corpuscle, glomeruli, and Bowman's capsule membranes significantly increased, and there was a significant decrease in the mean total volume of Bowman's space in the high-dose CeO group compared to that in the control group. No statistically significant differences existed in the serum levels of MDA and FRAP in the treated and control groups.

Conclusion: Our results suggest that high doses of CeO impair fetal renal development in pregnant mice, which results in kidney damage. Therefore, CeO administration during pregnancy could have dose-dependent adverse effects on the developing kidneys in neonates.
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http://dx.doi.org/10.1155/2020/9132724DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7330649PMC
May 2021

Protective effects of royal jelly on testicular torsion induced ischaemia reperfusion injury in rats.

Andrologia 2020 Oct 22;52(9):e13716. Epub 2020 Jun 22.

Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.

This study was performed to investigate the protective effects of royal jelly (RJ) on a testicular torsion-induced ischaemia/reperfusion (I/R) injury in adult rats. A total of 40 male Wistar rats were divided into four groups, including 10 rats in each group: Group 1 (sham), Group 2 (Control), group 3 (I/R rats treated with 100 mg/kg RJ for 50 days after torsion) and group 4( I/R rats treated with 20 mg/kg vitamin C for 50 days after torsion). Testicular torsion was created by rotating the right testes 720° a clockwise direction for 90 min. The levels of testosterone were measured by ELISA. Pathological evaluation, mean maturity and quality of the seminiferous tubules were used. Results showed that the testicular histopathology standards and testosterone levels changes were statistically significant in groups 3 and 4. The results obtained in this study may suggest that RJ like vitamin C had protective effects on a testicular ischaemia/reperfusion-induced injury in rats.
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http://dx.doi.org/10.1111/and.13716DOI Listing
October 2020

Determination of maternal risk factors of preterm delivery: adjusted for sparse data bias; results from a population-based case-control study in Iran.

Obstet Gynecol Sci 2020 Mar 11;63(2):117-125. Epub 2020 Feb 11.

Department of Biostatistics and Epidemiology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.

Objective: To determine the maternal risk factors associated with preterm delivery in Iran.

Methods: A population-based case-control study was conducted including 48 women having preterm delivery (case group) and 100 women having term delivery (control group) between March 2007 and March 2012 in the maternity hospitals of the Selseleh County, Lorestan province, Iran. Information regarding maternal risk factors was collected by structured interview and reviewing the medical records. The maternal risk factors associated with preterm delivery were identified using univariate and multivariable logistic regression analysis after adjusting the sparse data bias. The area under the receiver operating characteristic (ROC) curves was estimated to evaluate the discrimination power of the statistical models.

Results: Multivariable analysis demonstrated that multiparty (odds ratio [OR], 14.23; 95% confidence interval [CI], 1.60-127.05), history of gestational diabetes (OR, 0.10; 95% CI, 0.01-0.99), thyroid dysfunction (OR, 97.32; 95% CI, 5.78-1,637.80), urinary tract infection (OR, 16.60; 95% CI, 3.20-85.92), and taking care during pregnancy (OR, 0.12; 95% CI, 0.03-0.50) had significant impact on preterm delivery after adjusting the potential confounders. The area under the ROC curve for the aforementioned maternal risk factors was 0.86 (95% CI, 0.80-0.92).

Conclusion: Our study provides evidence for the associations between multiparty, history of gestational diabetes, thyroid dysfunction, urinary tract infection, as well as taking care during pregnancy, and preterm delivery.
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http://dx.doi.org/10.5468/ogs.2020.63.2.117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073365PMC
March 2020

Effects of Chitosan/Nano Selenium Biofilm on Infected Wound Healing in Rats; An Experimental Study.

Bull Emerg Trauma 2019 Jul;7(3):284-291

Department of Anatomical Sciences, Lorestan University of Medical Sciences, Kharamabad, Iran.

Objective: The present study was aimed at assessment of effect of application of Chitosan/Nano Selenium biofilm on infected wound healing in rats.

Methods: Sixty-eight male Wistar rats were randomized into four groups of 17 animals each. In group I (Normal) the wounds were created with no infection. In group II (MRSA), the wounds were infected with methicillin resistant MRSA). In group III (MRSA/CHIT), animals with infected wounds were dressed with chitosan biofilm only. In group IV (MRSA/CHIT/NS), animals with infected wounds were dressed with Chitosan/Nano Selenium biofilm.

Results: There were significant differences in comparisons of group IV and other groups, particularly in terms of cellular infiltration and neovascularization. During the study period, scores for neovascularization was significantly higher in group IV rats than other groups (<0.05). Polymorphonuclear (PMN) and mononuclear (MNC) cell count and fibroblast cell proliferation in group IV were significantly higher than those of other experimental groups (<0.05).

Conclusion: Chitosan/Nano Selenium biofilm resulted in significant improvement in histopathological indices in full thickness infected wound healing.
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http://dx.doi.org/10.29252/beat-0703012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6681872PMC
July 2019

Encapsulation of extract in alginate hydrogel accelerate wound healing in adult male rats.

Inflamm Regen 2019 30;39. Epub 2019 Jan 30.

6Tissue Engineering and Stem Cells Research Center, Shahroud University of Medical Sciences, Shahroud, Iran.

Background: Finding the best dressing for a specific had continued from the past to present. The aim of this study was to evaluate the of encapsulated extract of in hydrogel alginate at wound healing.

Methods: Thirty-two male Wistar rats with a puncture wound in the back of the neck skin were divided randomly into four groups including a control group, -treated group, hydrogel alginate-treated group, and encapsulated in hydrogel alginate-treated group. Rats were treated for 22 days. The skin samples were taken on 3rd, 7th, 14th, and 22nd days after treatment for light microscopy. Results were analyzed in accordance with Kruskal-Wallis and Friedman test (for histopathology analysis) by using SPSS v.22 software.

Results: Macroscopically evaluations and measurement of wound size showed increased wound healing process in the treated groups. The complete improvement was created on the 14th day. The wound site was not observed on the 22nd day. But the wound site was observed on the 22nd day in the control group. Also, comparison of the percentage of wound healing between the treated and control groups on 3rd, 7th, 14th, and 22nd days showed a significant difference ( < 0.05). Comparison of the H&E stained sections in the studied groups showed that treated groups were effective on wound healing in comparison with the control group.

Conclusions: Encapsulated extract of in hydrogel alginate may accelerate wound improvement and increase the rate of wound healing without scar formation.
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http://dx.doi.org/10.1186/s41232-019-0090-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6352332PMC
January 2019

The Antinociceptive Effects of Rosuvastatin in Chronic Constriction Injury Model of Male Rats.

Basic Clin Neurosci 2018 Jul-Aug;9(4):251-260. Epub 2018 Jul 1.

Nutritional Health Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.

Introduction: According to studies, statins possess analgesics and anti-inflammatory properties. In the present study, we examined the antinociceptive, anti-inflammatory and antioxidative effects of rosuvastatin in an experimental model of Chronic Constriction Injury (CCI).

Methods: Our study was conducted on four groups; sham, CCI (the control group), CCI+rosuvastatin (i.p. 5 mg/kg), and CCI+rosuvastatin (i.p. 10 mg/kg). We performed heat hyperalgesia, cold and mechanical allodynia tests on the 3, 7, 14, and 21 after inducing CCI. Blood samples were collected to measure the serum levels of Tumor Necrosis Factor (TNF)-α, and Interleukin (IL)-6. Rats' spinal cords were also examined to measure tissue concentration of Malondialdehyde (MDA), Superoxide Dismutase (SOD), and Glutathione Peroxidase (GPx) enzymes.

Results: Our findings showed that CCI resulted in significant increase in heat hyperalgesia, cold and mechanical allodynia on the 7, 14 and 21 day. Rosuvastatin use attenuated the CCI-induced hyperalgesia and allodynia. Rosuvastatin use also resulted in reduction of TNF-α, IL-6, and MDA levels. However, rosuvastatin therapy increased the concentration of SOD and GPx in the CCI+Ros (5 mg/kg) and the CCI+Ros (10 mg/kg) groups compared to the CCI group.

Conclusion: Rosuvastatin attenuated the CCI-induced neuropathic pain and inflammation. Thus, antinociceptive effects of rosuvastatin might be channeled through inhibition of inflammatory biomarkers and antioxidant properties.
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http://dx.doi.org/10.32598/bcn.9.4.251DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6276538PMC
July 2018

Application of Cell Therapy for Anti-Aging Facial Skin.

Curr Stem Cell Res Ther 2019 ;14(3):244-248

Faculty of Medicine, Department of Surgery, Lorestan University of Medical Science, Khoramabad, Iran.

The human skin undergoes the complex process of aging which is prompted by the interplay of intrinsic mechanisms and extrinsic influences. Aging is unavoidable but can be somewhat delayed. Numerous approaches have been developed to slow down facial skin aging process as it is of interest to stake holders in the beauty and fashion world as well as to plastic surgeons. Adipose-derived stem cell [ADSC] and mesenchymal stem cell [MSC] as potential anti-aging agents to some extent have provided a promising and effective alternative in managing skin and facial skin aging. Furthermore, bone marrow-derived mesenchymal stem cells [BMMSC] have exhibited similar ability to rejuvenate aged skin. This review is aimed at giving a comprehensive account of the application of stem cells especially ADSCs and MSCs to reduce or slow down the rate of facial skin aging process.
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http://dx.doi.org/10.2174/1574888X13666181113113415DOI Listing
August 2019

The Effect of Caffeic Acid on Spermatogonial Stem Cell-type A Cryopreservation.

Rep Biochem Mol Biol 2018 Oct;7(1):85-93

Razi Herbal Medicine Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.

Background: Cancer treatment methods can lead to male infertility .in this regard, cryopreservation of spermatogonial stem cells (SSC) and cell-to-person transplantation after the course of treatment to resolve the problem of infertility is a good one. The cryopreservation of SSC is an important process as it can help on the return of spermatogenesis. However, during this process, the stem cells often become damaged which degrades their value for experiments and treatments. Caffeic acid (CA) is an antioxidant that has been shown to increase the viability of cells under stress. The aim of this study was to investigate the effect of CA has on spermatogonial stem cell (SSC) cryopreservation.

Methods: Spermatogonial stem cells isolated from the testes of Balb/c mice pups were cultured in laminincoated dishes, purified using CD90.1 microbeads, then cryopreserved in vitrification media supplemented with 10 µM CA either through a slow or rapid freezing process. After thawing, cell viability was evaluated. Expression of Bax, Fas, Bcl-2 and P53 genes was determined by real-time PCR. Gel electrophoresis was used to confirm the results of the real-time PCR.

Results: The viability of the SSCs that were rapidly frozen and treated with CA was observed to be significantly reduced compared to the control group (p < 0.003). The viability SSCs that received CA and underwent the slow freezing treatment was significantly reduced compared to controls (p < 0.002). The expression levels of BAX, BCL-2, and Fas in the rapid freeze-thaw group didn't significantly change. However, the levels of P53 expression were shown to increase. In the group of SSCs that underwent the slow freezing process, the BAX gene expression levels increased, while the levels of BCL-2 gene expression decreased. No significant changes in the level of Fas and P53 expression were detected. When comparing the groups that received CA treatment, SSCs that were rapidly frozen showed an up-regulation of Fas and P53 expression and a down-regulation of Bcl-2 and Bax expression.

Conclusion: Caffeic acid may protect intact SCCs during the cryopreservation process through stimulating the induction of apoptosis in injured SSCs. Supplementing the vitrification media with CA has a superior effect on the preservation of SSCs.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6175585PMC
October 2018

Troxerutin protect sperm, seminiferous epithelium and pituitary-gonadal axis from torsion-detorsion injury: An experimental study.

Int J Reprod Biomed 2018 May;16(5):315-322

Department of Anatomical Sciences, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.

Background: Troxerutin is a flavonoid antioxidant that protect different organ against damage caused by ischemia-reperfusion

Objective: The aim of this study was to evaluate the effect of troxerutin in reducing the damages caused by ischemia-reperfusion in rat's testis.

Materials And Methods: 40 Male Wistar rats (2 month old) were divide to four groups (n=10). Group1 (sham), Group 2 (control, ischemia-reperfusion (I/R) without treatment), Group 3 (I/R+150 mg/kg of troxerutin), and group 4 (I/R+20 mg/kg of vitamin C). Treatment of group 3 and group 4 during torsion (twists 720 counter clock at 90 min) followed by 50 days detorsion. After 50 days, blood samples were collected and rats in all study groups were killed and their testes were removed, and fixed with Bouin's solution. Testis was stained with hematoxylin and eosin dye and the level of testosterone, luteinizing hormone (LH) and follicle-stimulating hormone (FSH) were measured with ELISEA methods. TUNEL was employed to detect apoptosis. Epididymis caudal part was removed and total sperm count was determined. Johnson techniques were used for assessment of seminiferous tubules quality.

Results: Troxerutin treated group has higher Johnson score's (p≤0.001), antiapoptotic properties (p≤0.001), sperm count (p=0.065), and higher LH (p≤0.001), FSH (p≤0.001) and testosterone (p=0.002) levels than control group. Vitamin C treated group showed increase level of testosterone but didn't show significant differences on the number of apoptotic cells, Johnson scores, LH, FSH and sperm count than control group.

Conclusion: Troxerutin has protective effects on testicular torsion induced injury and can ameliorate spermatogenesis in the torsion-detorsion models.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6046204PMC
May 2018

Protective effects of persian honey, Apis Mellifera Meda Skorikov on side effects of chemotherapy and ischemia/reperfusion induced testicular injury.

J Complement Integr Med 2018 May 23;15(4). Epub 2018 May 23.

School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.

Introduction The aim of the present study was to survey the protective effect of pretreatment with Persian honey on amelioration of side effects of chemotherapy and ischemia/reperfusion induced testicular injury. Materials and methods Forty adult's male wistar rats were divided into four groups of ischemia-reperfusion (IR), honey + ischemia-reperfusion (HIR), Busulfan (B) and Busulfan intraperitoneally+ honey (BH). The seminiferous tubules were rated for their modified spermatogenesis index (SI) by Johnsons score. Detection of single- and double-stranded DNA breaks at the early stages of apoptosis was performed using the in-situ cell death detection kit. Total serum concentration of Follicle-stimulating hormone (FSH) , Luteinizing hormone (LH) and testosterone was measured using ELISA. All data were expressed as mean ± SD and significance was set at p≤0.05. Results Honey improved SI in the HIR and BH groups and serum levels of FSH and LH in the BH and HIR groups (p<0.001). Also, serum levels of testosterone were significantly higher in BH and HIR groups. But, apoptotic cells in IR and B groups significantly increased (p<0.001), while in HIR and BH groups, the number of apoptotic cells decreased and the positive cells of TUNEL (TdT-mediated dUTP-X nick end labelling) staining were detected in spermatocytes and spermatid. Discussion Pretreatment with honey protect testis against chemotherapy and testicular IR injury, increase FSH and LH and testosterone and decrease the cellular damage and apoptosis. Honey can decrease the side effects of chemotherapy on reproductive system and prevent sterility.
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http://dx.doi.org/10.1515/jcim-2016-0035DOI Listing
May 2018

Skin Mast Cell Promotion in Random Skin Flaps in Rats using Bone Marrow Mesenchymal Stem Cells and Amniotic Membrane

Iran Biomed J 2018 09 6;22(5):322-30. Epub 2018 Mar 6.

Department of Surgery, Lorestan University of Medical Sciences, Khorramabad, Iran.

Background: Skin flap procedures are employed in plastic surgery, but failure can lead to necrosis of the flap. Studies have used bone marrow mesenchymal stem cells (BM-MSCs) to improve flap viability. BM-MSCs and acellular amniotic membrane (AAM) have been introduced as alternatives. The objective of this study was to evaluate the effect of BM-MSCs and AAM on mast cells of random skin flaps (RSF) in rats.

Methods: RSFs (80 × 30 mm) were created on 40 rats that were randomly assigned to one of four groups, including (I) AAM, (II) BM-MSCs, (III) BM-MSCs/AAM, and (IV) saline (control). Transplantation was carried out during the procedure (zero day). Flap necrosis was observed on day 7, and skin samples were collected from the transition line of the flap to evaluate the total number and types of mast cells. The development and the total number of mast cells were related to the development of capillaries.

Results: The results of one-way ANOVA indicated that there was no statistically significant difference between the mean numbers of mast cell types for different study groups. However, the difference between the total number of mast cells in the study groups was statistically significant (p = 0.001).

Conclusion: The present study suggests that the use of AAM/BM-MSCs can improve the total number of mast cells and accelerate the growth of capillaries at the transient site in RSFs in rats.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6058190PMC
http://dx.doi.org/10.29252/ibj.22.5.322DOI Listing
September 2018

Minocycline through attenuation of oxidative stress and inflammatory response reduces the neuropathic pain in a rat model of chronic constriction injury.

Iran J Basic Med Sci 2018 Feb;21(2):138-144

Department of Pathology, Lorestan University of Medical Sciences, Khorramabad, Iran.

Objectives: Several lines of evidence showed that minocycline possesses antioxidant and anti-inflammatory properties. This study aimed to demonstrate the effects of minocycline in rats subjected to chronic constriction injury (CCI).

Materials And Methods: In this study four groups (n = 6-8) of rats were used as follows: Sham, CCI, CCI + minocycline (MIN) 10 mg/Kg (IP) and CCI + MIN 30 mg/Kg (IP). On days 3, 7, 14, and 21 post-surgery hot-plate, acetone, and von Frey tests were carried out. Finally, Motor Nerve Conduction Velocity Evaluation (MNCV) assessment was performed and spinal cords were harvested in order to measure tissue concentrations of TNF_α, IL-1β, Glutathione peroxidase (GPx), Superoxide dismutase (SOD) and Malondialdehyde (MDA). Extent of perineural inflammation and damage around the sciatic nerve was histopathologically evaluated.

Results: Our results demonstrated that CCI significantly caused hyperalgesia and allodynia twenty-one days after CCI. MIN attenuated heat hyperalgesia, cold and mechanical allodynia and MNCV in animals. MIN also decreased the levels of TNF_α and IL-1β. Antioxidative enzymes (SOD, MDA, and GPx) were restored following MIN treatment. Our findings showed that MIN decreased perineural inflammation around the sciatic nerve. According to the results, the neuropathic pain reduced in the CCI hyperalgesia model using 30 mg/kg of minocycline.

Conclusion: It is suggested that antinociceptive effects of minocycline might be mediated through the inhibition of inflammatory response and attenuation of oxidative stress.
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http://dx.doi.org/10.22038/IJBMS.2017.24248.6053DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5811752PMC
February 2018

Stem cell and skin rejuvenation.

J Cosmet Laser Ther 2018 Jun 7;20(3):193-197. Epub 2018 Feb 7.

b Assistant Professor, Faculty of Medicine, Department of Surgery , Lorestan University of Medical Sciences , Khorramabad , Iran.

Stem cell-based therapies have been widely used for their abilities to repair and regenerate different types of tissues and organs in cosmetic and plastic surgeries. It involves the clinical application of different types of stem cells. Different stem cells have been reported to be applicable in different areas of cosmetic surgeries like face lipoatrophy, skin rejuvenation, breast enhancement, and body contouring. However, adipose-derived stem cells remain the most widely used by cosmetic surgeons as they have the potential and capability to differentiate into mesenchymal, ectodermal, and endodermal lineages and are easily accessible to harvest. The purpose of this review is to summarize available applications of stem in cosmetic and plastic surgeries.
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http://dx.doi.org/10.1080/14764172.2017.1383615DOI Listing
June 2018

Distribution of Class D Carbapenemase and Extended-Spectrum β-Lactamase Genes among Isolated from Burn Wound and Ventilator Associated Pneumonia Infections.

J Clin Diagn Res 2017 Jul 1;11(7):DC19-DC23. Epub 2017 Jul 1.

Razi Herbal Medicines Research Center, Department of Microbiology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.

Introduction: Resistance to is dramatically on the rise in Iran. Therefore, it is important to study resistance pattern among isolates which is a common cause of nosocomial infections.

Aim: To investigate antibiotic resistance patterns and the role of resistant genes and biofilm formation in the induction of resistance among isolated from burn wound and ventilator associated pneumonia infections.

Materials And Methods: Total 103 isolates such as 33 burn samples from Rasool Akram Hospital and 70 isolates from ventilated patients in Shahid Motahhari Hospital were identified with . using biochemical method, and then identified to species level with PCR of and gene. Antibiotic sensitivity pattern for β-lactam and carbapenem antibiotics was assessed using Agar disc diffusion test and E-test. The presence of different carbapenemase and metalo-β-lactamase (, , , , , , , , , , ), extended-spectrum β-lactamases (, ) and two insertion sequences genes (, ) was assessed. Biofilm formation of all isolates was then assessed. Chi-square analysis or Fisher's-exact tests were used for statistical analysis. A p-value <0.05 was considered statistically significant.

Results: Colistin was the most effective antimicrobial agents, although 10.7% (11/103) of the isolates were resistant. The high rate of resistance to meropenem (93.2%) and imipenem (90.3%) was determined. Also, with exception of ampicillin-sulbactam, surprisingly the resistant rate was 28.2%, the resistance to β-lactam antibiotic was dramatically increased. Co-existence of two and three blaOXA genes was also determined. The blaOXA-58 was detected in only one isolate. The blaTEM and blaOXA-23 was the most prevalent Extended-Spectrum β-Lactamases (ESBL) gene. All isolates were biofilm producers.

Conclusion: Antibiotic resistance is increasing among A. baumannii isolates which is due to excessive use of antibiotics and also acquired resistant genes and biofilm production. Resistance to nearly all antimicrobial agents especially colistin as end choice for treatment of multiple drug resistance A. baumannii is a big concern.
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http://dx.doi.org/10.7860/JCDR/2017/25534.10218DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5583823PMC
July 2017

Effects of pomegranate peel extract on histopathology, testosterone levels and sperm of testicular torsion-detorsion induced in adult Wistar rats.

J Complement Integr Med 2017 Jul 22;14(4). Epub 2017 Jul 22.

.

Background In the present study, effects of pomegranate peel extract have been evaluated on decreasing the damage induced by testis torsion. Methods In this study, 30 adult Wistar rats were randomly divided into three groups of control, experimental (1) and experimental (2).

Control: no ischemia, received vehicle alone, exposed to sham operation. Experimental (1): Received the vehicle alone during ischemia followed by 60 days' reperfusion. Experimental (2): After performing ischemia reperfusion, 500 mg/kg of pomegranate peel extract has been used for 60 days. Blood samples and sperm samples were collected. Testes were harvested and stained with haematoxylin and eosin to study the structure of seminiferous tubules. Results The statistical comparison between sperm count and their viability and testosterone hormone amount showed a significant difference between control and experimental (1) groups and control and experimental (2) groups. The results showed an improvement of morphological condition of seminiferous tubules. Conclusions Pomegranate peel extract has revealed desirable changes on the effective parameters in infertility.
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http://dx.doi.org/10.1515/jcim-2017-0009DOI Listing
July 2017

Evaluation of selenium on kidney function following ischemic injury in rats; protective effects and antioxidant activity.

J Renal Inj Prev 2017 24;6(2):93-98. Epub 2016 Nov 24.

Department of Pharmacotherapy, Faculty of pharmacy, Lorestan University of Medical Sciences, Khorramabad, Iran.

Renal dysfunction is caused by ischemia-reperfusion (I/R) injury, which is a common problem in kidney surgery or kidney transplantation. The human body consists of enormous complex antioxidant systems, which inquires adequate selenium (Se) absorption for normal physiologic function. It is known that Se has some antioxidant effects. In the present research, effects of the Se on damages caused by I/R injury investigated. In this experimental research, four groups of rats (weighing 220±10 g) used, include control group, I/R group, healthy group treated with Se for two weeks, and I/R group with two-week Se treatment. On the test day, I/R was treated in both right and left renal arteries for 45 minutes and the reperfusion was done for 24 hours. In I/R group, the amount of urea and serum creatinine (Cr) was an injury indicator of the kidney cells which showed a significant increase compared with the control group. When the treatment with Se significantly reduced these indicators, glutathione (GSH) enzyme levels reduced significantly in the second group and the enzyme levels increased due to Se treatment in the fourth group. Furthermore, malondialdehyde (MDA) enzyme levels increased in I/R group due to the Se treatment in the fourth group which was significantly reduced. In addition, the tissue damage was reduced in the fourth group compared with I/R group. Se has a protective effect against the I/R injury. This effect might be due to the antioxidant properties of Se.
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http://dx.doi.org/10.15171/jrip.2017.18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5423291PMC
November 2016

Selenium Ameliorate Peripheral Nerve Ischemic-Reperfusion Injury via Decreased TNF-α.

Biol Trace Elem Res 2017 Apr 6;176(2):328-337. Epub 2016 Sep 6.

Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.

Selenium is considered as a trace element that plays antioxidant role in the body. So, the aim of this study was to evaluate the effect of selenium on ameliorating of sciatic nerve ischemia-reperfusion injury. Eighty (80) adult male Wistar rats weighing 250-300 g were used. They were divided into 10 groups (n = 8). Then, femoral vessels were obstructed by using 4/0 silk and splitknot techniques. After 3-h ischemia for all the groups, reperfusion was applied for different periods: 3, 7, 14, and 28 days. In half of each experimental group, 0.2 mg/kg selenium was injected intraperitoneally, coinciding with ischemia. After reperfusion, according to the grouping, rats were killed by using high dose of anesthetic drug and then sciatic nerve was removed and fixed. Then, tissue samples were processed and subsequently stained with hematoxylin-eosin, apoptosis, and immunohistochemistry stains. On the third day of reperfusion, the amount of TNF-α as an inflammatory marker of ischemia-reperfusion acute phase increased. On the seventh day of reperfusion, the amount of NF-кB as an apoptotic index and infiltration of mast cells increased in the tissue as a result of development of inflammation. But, on the 14th day of reperfusion, the amount of NF-кB as an apoptotic index decreased to the lowest amount. On the 28th day of reperfusion, the amount of TNF-α as an inflammatory marker decreased to its lowest level. Prescription of selenium concurrent with development of ischemia can reduce the damage caused by sciatic nerve ischemia-reperfusion.
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http://dx.doi.org/10.1007/s12011-016-0836-7DOI Listing
April 2017

Solid phase extraction of Cd(II) and Pb(II) ions based on a novel functionalized [email protected] SiO2 core-shell nanoparticles with the aid of multivariate optimization methodology.

Mater Sci Eng C Mater Biol Appl 2015 Apr 6;49:416-421. Epub 2015 Jan 6.

Young Researchers and Elite Club, Karaj Branch, Islamic Azad University, P.O. Box 31485-313, Karaj, Iran.

This work describes novel [email protected] core-shell nanoparticles functionalized with phenyl isothiocyanate and its application in the preconcentration of Cd(II) and Pb(II) ions. The parameters affecting the preconcentration procedure were optimized by a Box-Behnken design through response surface methodology. Three variables (extraction time, magnetic sorbent amount, and pH value) were selected as the main factors affecting the sorption step, while four variables (type, volume and concentration of the eluent; and elution time) were selected as effective factors of elution step in the optimization study. Following the sorption and elution, the ions were quantified by FAAS. The limits of detection were 0.05 and 0.9ngmL(-1) for Cd(II) and Pb(II) ions, respectively. The relative standard deviations were less than 6.4%. The sorption capacity (in mg g(-1)) of this new sorbent is 179 for Cd(II) and 156 for Pb(II). Finally, this nanocomposite was successfully applied to the rapid extraction of trace quantities of heavy metal ions from fish, sediment, soil, and water samples and satisfactory results were obtained.
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http://dx.doi.org/10.1016/j.msec.2015.01.013DOI Listing
April 2015

Comparison of outcomes using diced cartilage with or without tensor fascia latae wrapping in rabbits.

Aesthet Surg J 2014 Sep 25;34(7):NP50-60. Epub 2014 Apr 25.

Dr Hafezi is Professor of Plastic Surgery, Iran University of Medical Sciences, St Fatima Hospital, and Burn Research Center, Tehran, IranDr Abbaszadeh is a plastic surgery fellow, Iran University of Medical Sciences, St Fatima Hospital, and Burn Research Center, Tehran, IranDrs Ashtiani and Fatemi are Associate Professors of Plastic Surgery at the Iran University of Medical Sciences, St Fatima Hospital, and Burn Research Center, Tehran, IranDr Naghibzadeh is Professor of Ear, Nose, and Throat Surgery at Shahid Beheshty University of Medical Sciences, Loghman Hakim Hospital, Tehran, IranDr Nouhi is a pathologist at Kowsar Laboratory, Tehran, Iran.

Background: Diced cartilage has been associated with several advantages and rewarding results, leading to its widespread application in various forms in rhinoplasty, but the outcomes of diced cartilage with and without tensor fascia latae wrapping have not been widely reported in evidence-based articles.

Objectives: The authors compared changes in weight, size, and histology of both bare and fascia-wrapped diced cartilage in rabbits as a model for human surgical outcomes.

Methods: One auricle from each of 15 rabbits was divided into 2 pieces, and both samples were diced. The tensor fascia latae of the rabbit was wrapped around 1 cartilage specimen from each rabbit (group A specimen); the other specimen received no wrapping (group B specimen). A group A specimen and a group B specimen were implanted into separate subcutaneous pockets in each rabbit. After 3 months, samples were removed from the recipient beds and weighed to compare pre- and postoperative weights. All specimens were examined for evidence of cartilage viability by histologic methods.

Results: There was a significant decrease in the weight of cartilage in group A. A nonsignificant weight increase was observed in group B. Histologic analyses of 5 parameters revealed no significant differences between the 2 groups, except for a significantly greater amount of new cartilage formation in group B.

Conclusions: Any type of wrapping around diced cartilage may inhibit its access to surrounding nutrients. Dicing increases the absorption surface, which may result in some overgrowth, but wrapping may have a negative impact on the viability of the diced cartilage.

Level Of Evidence: 4.
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http://dx.doi.org/10.1177/1090820X14529076DOI Listing
September 2014

Bis(ethyl-enediamine-κ(2)N,N')bis-(methanol-κO)copper(II) benzene-1,4-dicarboxyl-ate methanol disolvate.

Acta Crystallogr Sect E Struct Rep Online 2012 Jul 30;68(Pt 7):m1012. Epub 2012 Jun 30.

Department of Chemistry, Shahid Beheshti University, G. C., Evin, Tehran 1983963113, Iran.

In the cation of the title compound, [Cu(C(2)H(8)N(2))(2)(CH(3)OH)(2)](C(8)H(4)O(4))·2CH(3)OH, the Cu(II) atom lies on an inversion centre. The four N atoms of two ethyl-enediamine ligands around the Cu(II) atom form the equatorial plane, while two methanol O atoms in the axial positions complete a Jahn-Teller distorted octa-hedral coordination. The benzene-1,4-dicarboxyl-ate anion is centrosymmetric. In the crystal, C-H⋯O, N-H⋯O and O-H⋯O hydrogen bonds link the cations, the anions and the methanol solvent mol-ecules.
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http://dx.doi.org/10.1107/S1600536812028942DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3393248PMC
July 2012
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