Publications by authors named "Abdul Hai"

66 Publications

Valorization of groundnut shell via pyrolysis: Product distribution, thermodynamic analysis, kinetic estimation, and artificial neural network modeling.

Chemosphere 2021 Nov 15;283:131162. Epub 2021 Jun 15.

Department of Chemical Engineering, Khalifa University, P.O. Box 127788, Abu Dhabi, United Arab Emirates. Electronic address:

Pyrolysis of agricultural biomass is a promising technique for producing renewable energy and effectively managing solid waste. In this study, groundnut shell (GNS) was processed at 500 °C in an inert gas atmosphere with a gas flow rate and a heating rate of 10 mL/min and 10 °C/min, respectively, in a custom-designed fluidized bed pyrolytic-reactor. Under optimal operating conditions, the GNS-derived pyrolytic-oil yield was 62.8 wt.%, with the corresponding biochar (19.5 wt.%) and biogas yields (17.7 wt.%). The GC-MS analysis of the GNS-based bio-oil confirmed the presence of (trifluoromethyl)pyridin-2-amine (18.814%), 2-Fluoroformyl-3,3,4,4-tetrafluoro-1,2-oxazetidine (16.23%), 5,7-dimethyl-1H-Indazole (11.613%), N-methyl-N-nitropropan-2-amine (6.5%) and butyl piperidino sulfone (5.668%) as major components, which are used as building blocks in the biofuel, pharmaceutical, and food industries. Furthermore, a 2 × 5 × 1 artificial neural network (ANN) architecture was developed to predict the decomposition behavior of GNS at heating rates of 5, 10, and 20 °C/min, while the thermodynamic and kinetic parameters were estimated using a non-isothermal model-free method. The Popescu method predicted activation energy (E) of GNS biomass ranging from 111 kJ/mol to 260 kJ/mol, with changes in enthalpy (ΔH), Gibbs-free energy (ΔG), and entropy (ΔS) ranging from 106 to 254 kJ/mol, 162-241 kJ/mol, and -0.0937 to 0.0598 kJ/mol/K, respectively. The extraction of high-quality precursors from GNS pyrolysis was demonstrated in this study, as well as the usefulness of the ANN technique for thermogravimetric analysis of biomass.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131162DOI Listing
November 2021

Hybrid capacitive deionization of NaCl and toxic heavy metal ions using faradic electrodes of silver nanospheres decorated pomegranate peel-derived activated carbon.

Environ Res 2021 06 14;197:111110. Epub 2021 Apr 14.

Department of Chemical Engineering, Khalifa University, P.O. Box 127788, Abu Dhabi, United Arab Emirates. Electronic address:

Capacitive deionization (CDI) is an evolving technology for eradicating salt and toxic heavy metal ions from brackish wastewater. However, traditional CDI electrodes have lower salt adsorption capacity and inadequate adsorption of selective metal ions for long-term operations. Herein, Ag nanospheres incorporated pomegranate peel-derived activated carbon (Ag/P-AC) was prepared and implied to the CDI process for removing NaCl, toxic mono-, di-, and trivalent metal ions. Morphological analysis revealed that the 80-100 nm-sized Ag nanospheres were uniformly decorated on the surfaces of P-AC nanosheets. The Ag/P-AC has a higher specific surface area (640 m g), superior specific capacitance (180 F g at 50 mV s) and a lower charge transfer resistance (0.5 Ω cm). CDI device was fabricated by Ag/P-AC as an anode, which adsorbed anions and P-AC as cathode for adsorption of positively charged ions at 1.2 V in an initial salt concentration of 1000 mg L. An asymmetric Ag/P-AC//P-AC exhibited a maximum NaCl adsorption capacity of 36 mg g than symmetric P-AC//P-AC electrodes (22.7 mg g). Furthermore, Pb(II), Cd(II), F, and As(III) ions were successfully removed from simulated wastewater by using Ag/P-AC//P-AC based CDI system. These asymmetric CDI-electrodes have an excellent prospect for the removal of salt and toxic contaminants in industrial wastewater.
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http://dx.doi.org/10.1016/j.envres.2021.111110DOI Listing
June 2021

Designed assembly of Ni/MAX (TiAlC) and porous graphene-based asymmetric electrodes for capacitive deionization of multivalent ions.

Chemosphere 2021 Mar 21;266:129048. Epub 2020 Nov 21.

Department of Chemical Engineering, Khalifa University, P.O. Box 127788, Abu Dhabi, United Arab Emirates. Electronic address:

The contamination of aquatic ecosystems by fluoride and heavy metal ions constitute an environmental hazard and has been proven to be harmful to human health. This study explores the feasibility of using asymmetric capacitive deionization (CDI) electrodes to remove such toxic ions from wastewater. An asymmetric CDI cell was fabricated using 2D Ni/MAX as an anode and 3D porous reduced graphene oxide (pRGO) as a cathode for the electrosorption of F, Pb, and As(III) ions. A simple microwave process was used for the synthesis of Ni/MAX composite using fish sperm DNA (f-DNA) as a cross-linker between MAX nanosheets (NSs) and the metallic Ni nanoparticles (NPs). Further, pRGO anode was prepared through effective reduction of RGO using lemon juice as green reducing agent with the assist of f-DNA as a structure-directing agent for the formation of 3D network. With this tailored nanoarchitecture, pRGO and Ni/MAX electrodes exhibited a high specific capacitance of 760 and 385 F g, respectively. The fabricated Ni/MAX and pRGO based CDI system demonstrated a high electrosorption capacity of 68, 76, and 51 mg g for the monovalent F, divalent Pb, and trivalent As(III) ions at 1.4 V in neutral pH. Furthermore, Ni/MAX//pRGO system was successfully applied for the removal of total F(T), Pb(T), and As(T) ions from real industrial wastewater and contaminated groundwater. The present findings indicate that the fabricated Ni/MAX//pRGO electrode has excellent electrochemical properties that can be exploited for the removal of anionic and cationic metal ions from aqueous solutions in a CDI based system.
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http://dx.doi.org/10.1016/j.chemosphere.2020.129048DOI Listing
March 2021

Adermatoglyphia.

Natl Med J India 2019 Jul-Aug;32(4):253

Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, Northern Border University, Arar, Saudi Arabia.

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http://dx.doi.org/10.4103/0970-258X.291296DOI Listing
August 2020

Enhanced electrochemical performances of peanut shell derived activated carbon and its FeO nanocomposites for capacitive deionization of Cr(VI) ions.

Sci Total Environ 2019 Nov 6;691:713-726. Epub 2019 Jul 6.

Department of Nanoscience and Technology, Bharathiar University, Coimbatore 641 046, Tamil Nadu, India.

Capacitive deionization (CDI) is one of the most efficient and emerging techniques for the removal of toxic metal ions from aqueous solutions. In this study, mesoporous peanut shell derived activated carbon (PSAC) was prepared by low temperature pyrolysis at 500 °C. Subsequently, a novel iron oxide/PSAC (FeO/PSAC) nanocomposite adsorbent was prepared via facile one-pot hydrothermal synthesis method at 180 °C. Nucleation growth mechanism and appropriate characterizations of prepared nanocomposites were investigated. The obtained FeO/PSAC possessed a highly mesoporous structure, and a large specific surface area (680 m/g). The electrochemical analysis showed that the obtained FeO/PSAC nanocomposites exhibited higher capacitance (610 F/g at 10 mV/s), good stability and low internal resistance. A batch mode adsorption and CDI based Cr(VI) removal studies were conducted. Effects of solution pH and cycle time on Cr(VI) electrosorption capacity were further investigated. The FeO/PSAC based electrodes exhibit a maximum electrosorption capacity of 24.5 mg/g at 1.2 V, which was remarkably larger than other reported materials. The fabricated composite displayed higher electrosorption capacity with rapid time and a favorable reduction of Cr (VI) to Cr(III). Studies indicated that the FeO/PSAC based CDI electrode possesses a good potential to be applied for the removal of toxic metal ions from wastewater.
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http://dx.doi.org/10.1016/j.scitotenv.2019.07.069DOI Listing
November 2019

Two men with dyspnea, enlarged lymph nodes · Dx?

J Fam Pract 2016 12;65(12):916-920

Division of Internal Medicine, Kaplan Medical Center, Rehovot, Israel. Email:

Systolic heart failure has been previously recognized as a cause of reversible mediastinal lymphadenopathy (MLN). Other causes of MLN include sarcoidosis, various malignancies, pulmonary infections, and occupational lung diseases. There are, however, no reports of MLN in patients with diastolic heart failure.
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December 2016

Increase in activity of Na, K-ATPase by Porphyrin compounds as treatment for Dysnatremias caused by Diabetes Mellitus.

Pak J Med Sci 2016 Sep-Oct;32(5):1131-1134

Nadeem Kizilbash, Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, Northern Border University, Arar, Saudi Arabia.

Objective: The aim of this study was to test the action of Porphyrin compounds, Tetraphenylporphine sulfonate (TPPS), 5,10,15,20-Tetrakis (4-sulfonatophenyl) porphyrinato Iron(III) Chloride (FeTPPS) and 5,10,15,20-Tetrakis (4-sulfonatophenyl) porphyrinato Iron(III) nitrosyl Chloride (FeNOTPPS), on Na+, K+ -ATPase of cell membrane of erythrocytes.

Methods: Enzymatic assays, measuring the amount of inorganic phosphate produced, were used to estimate the activity of Na, K-ATPase.

Results: The results show that Porphyrin compounds exert an insulin-like effect on Na, K-ATPase. They act by increasing the activity of the membrane-bound enzyme.

Conclusion: All the three Porphyrin compounds increased the activity of erythrocyte Na, K-ATPase. The exact mechanism of action of these compounds is not clear.
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http://dx.doi.org/10.12669/pjms.325.11530DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5103120PMC
November 2016

Synthesis and characterization of polyurethane-cellulose acetate blend membrane for chromium (VI) removal.

Carbohydr Polym 2016 Nov 4;153:582-591. Epub 2016 Aug 4.

Department of Textile Engineering and Technology, University of the Punjab, Quaid-e-Azam Campus, P.O. Box 54590, Lahore, Pakistan; Department of Polymer Engineering and Technology, University of the Punjab, Quaid-e-Azam Campus, P.O. Box 54590, Lahore, Pakistan.

Blended membranes of polyurethane and cellulose acetate were prepared, characterized and investigated for their performance. Various ratios of cellulose acetate were employed to prepare four different blend membranes. The characteristics of both pure and blend membranes were investigated and results were compared to distinguish their properties. Functional group analysis was carried out by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) of pure and blend samples. Contact angle measurement and water content were evaluated to determine the membrane hydrophilicity. Moreover, the membrane morphology was studied by scanning electron microscopy (SEM). The membrane permeation properties and ability to reject chromium (VI) ions were tested at various pH and pressure by utilizing different salt concentrations.
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http://dx.doi.org/10.1016/j.carbpol.2016.08.011DOI Listing
November 2016

How does esophagus look on barium esophagram in pediatric eosinophilic esophagitis?

Abdom Radiol (NY) 2016 08;41(8):1466-73

Department of Radiology, King Fahad Medical City, Riyadh, 11525, Kingdom of Saudi Arabia.

Purpose: The clinical, endoscopic, and histologic findings of eosinophilic esophagitis (EoE) are well characterized; however, there have been very limited data regarding the radiologic findings of pediatric EoE. We report on the radiologic findings of pediatric EoE observed on barium esophagram and correlate them with the endoscopic findings.

Methods And Materials: We identified children diagnosed with EoE in our center from 2004 to 2015. Two pediatric radiologists met after their independent evaluations of each fluoroscopic study to reach a consensus on each case. Clinical and endoscopic data were collected by retrospective chart review.

Results: Twenty-six pediatric EoE cases (age range 2-13 years; median 7.5 years) had barium esophagram done as part of the diagnostic approach for dysphagia. Thirteen children had abnormal radiologic findings of esophagus (50%): rings formation (n = 4), diffuse irregularity of mucosa (n = 8), fixed stricture formation (n = 3), and narrow-caliber esophagus (n = 10). Barium esophagram failed to show one of 10 cases of narrow-caliber esophagus and 10 of 14 cases of rings formation visualized endoscopically. The mean duration of symptoms prior to diagnosis of EoE was longer (3.7 vs. 1.7 year; p value 0.019), and the presentation with intermittent food impaction was commoner in the group with abnormal barium esophagram as compared to the group with normal barium esophagram (69% vs. 8%; p value 0.04).

Conclusion: Barium swallow study is frequently normal in pediatric EoE. With the exception of narrow-caliber esophagus, our data show poor correlation between radiologic and endoscopic findings.
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http://dx.doi.org/10.1007/s00261-016-0712-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4972850PMC
August 2016

Spatial distribution and contamination assessment of six heavy metals in soils and their transfer into mature tobacco plants in Kushtia District, Bangladesh.

Environ Sci Pollut Res Int 2016 Feb 21;23(4):3414-26. Epub 2015 Oct 21.

Department of Applied Chemistry and Chemical Technology, Islamic University, Kushtia, 7003, Bangladesh.

Although the tobacco production and consumption rate in Bangladesh is very high and a substantial portion of premature deaths is caused by tobacco smoking, the status of heavy metals in tobacco plants has not yet determined. This study, therefore, investigated the concentrations of Cu, Ni, Cd, Pb, Cr, and Zn in tobacco plants and their surrounding agricultural soils in Kushtia District, Bangladesh. The geochemical maps showed a similar spatial distribution pattern of the analyzed metals and identified Shempur, Kharara, Taragunia, and Shantidanga as metal hot spots. Geoanalytical indexes were applied to assess the extent of soil contamination, and the results depicted that the soils of Shempur, Kharara, Taragunia, and Shantidanga were moderately contaminated where Cd contributed the most to contamination degree (C d) in spite of its relative low content. However, other five areas in Kushtia District were suggested as uncontaminated according to both C d and pollution load index (PLI). The hazard quotient (HQ) and hazard index (HI) showed no possible indication of human health risks via ingestion of agricultural soils. This study also determined that human activities such as excess application of commercial fertilizers, animal manures, and metal-based pesticides were the sources of Cu, Ni, Cd, and Cr enrichment in soils and that the metals into tobacco plants were transported from the soils. The present study conclusively suggested that regulation of improper use of agrochemicals and continuous monitoring of heavy metals in tobacco plants are needed to reduce the tobacco-related detrimental health problems in Bangladesh.
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http://dx.doi.org/10.1007/s11356-015-5575-3DOI Listing
February 2016

Influence of gut bacteria on development and progression of non-alcoholic fatty liver disease.

World J Hepatol 2015 Jun;7(12):1679-84

Ali Abdul-Hai, Ali Abdallah, Stephen DH Malnick, Division of Internal Medicine, Kaplan Medical Center, Affiliated to the Hebrew University, Rehovot 76100, Israel.

The intestine of the human contains a dynamic population of microbes that have a symbiotic relationship with the host. In addition, there is an effect of the intestinal microbiota on metabolism and digestion. Non-alcoholic fatty liver disease (NAFLD) is a common cause worldwide of hepatic pathology and is thought to be the hepatic manifestation of the metabolic syndrome. In this review we examine the effect of the human microbiome on the components and pathogenesis of the metabolic syndrome. We are now on the threshold of therapeutic interventions on the human microbiome in order to effect human disease including NAFLD.
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http://dx.doi.org/10.4254/wjh.v7.i12.1679DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4483549PMC
June 2015

Combining three antibodies nullifies feedback-mediated resistance to erlotinib in lung cancer.

Sci Signal 2015 Jun 2;8(379):ra53. Epub 2015 Jun 2.

Department of Biological Regulation, Weizmann Institute of Science, Rehovot 76100, Israel.

Despite initial responses to targeted kinase inhibitors, lung cancer patients presenting with primary epidermal growth factor receptor (EGFR) mutations acquire resistance, often due to a second-site mutation (T790M). However, clinical trials found no survival benefits in patients treated with a monoclonal antibody (mAb) to EGFR that should block activation of the mutated receptor and thus bypass resistance to molecules that target the catalytic or ATP-binding site. Using cell lines with the T790M mutation, we discovered that prolonged exposure to mAbs against only the EGFR triggered network rewiring by (i) stimulating the extracellular signal-regulated kinase (ERK) pathway; (ii) inducing the transcription of HER2 (human epidermal growth factor receptor 2) and HER3, which encode other members of the EGFR family, and the gene encoding HGF, which is the ligand for the receptor tyrosine kinase MET; and (iii) stimulating the interaction between MET and HER3, which promoted MET activity. Supplementing the EGFR-specific mAb with those targeting HER2 and HER3 suppressed these compensatory feedback loops in cultured lung cancer cells. The triple mAb combination targeting all three receptors prevented the activation of ERK, accelerated the degradation of the receptors, inhibited the proliferation of tumor cells but not of normal cells, and markedly reduced the growth of tumors in mice xenografted with cells that were resistant to combined treatment with erlotinib and the single function-blocking EGFR mAb. These findings uncovered feedback loops that enable resistance to treatment paradigms that use a single antibody and indicate a new strategy for the treatment of lung cancer patients.
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http://dx.doi.org/10.1126/scisignal.aaa0725DOI Listing
June 2015

Transplant of ex vivo incubated bone marrow with rIL -7 for the enhancement of immuno-hematopoietic reconstitution.

Leuk Lymphoma 2015 ;56(12):3387-92

a Department of Bone Marrow Transplantation & Cancer Immunotherapy , Hadassah/Hebrew University Medical Center , Kiryat Hadassah, Jerusalem , Israel.

Interleukin-7 (IL-7) is a critical cytokine in early B and T cell development. Peripheral T cell expansion and thymopoiesis and is a result of the ongoing reconstitution from uncommitted stem cells after transplant. We investigated the efficacy of ex vivo incubated bone marrow cells treated with recombinant human IL-7 (rIL-7) on subsequent in vivo murine models of syngeneic bone marrow (BM) transplant. After ex vivo culture with rIL-7, we observed a 1½-fold increase in BM cellularity; this increase was associated with an enhanced reconstitution of bone marrow cells and thymocytes at 45 days post-transplant. In addition to increased cellularity, lymphocytes from mice transplanted with cultured rIL-7 showed enhanced proliferative responses to mitogenic stimulation. These findings suggest rIL-7 to be a promising agent for the clinical application of treating immune deficiency and enhancing immuno-hematopoietic reconstitution of the stem cell auto/allograft.
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http://dx.doi.org/10.3109/10428194.2015.1016935DOI Listing
September 2016

An antibody to amphiregulin, an abundant growth factor in patients' fluids, inhibits ovarian tumors.

Oncogene 2016 Jan 27;35(4):438-47. Epub 2015 Apr 27.

Department of Biological Regulation, Weizmann Institute of Science, Rehovot, Israel.

Growth factors of the epidermal growth factor (EGF)/neuregulin family are involved in tumor progression and, accordingly, antibodies that intercept a cognate receptor, epidermal growth factor receptor (EGFR)/ERBB1, or a co-receptor, HER2, have been approved for cancer therapy. Although they might improve safety and delay onset of chemoresistance, no anti-ligand antibodies have been clinically approved. To identify suitable ligands, we surveyed fluids from ovarian and lung cancer patients and found that amphiregulin (AREG) is the most abundant and generalized ligand secreted by advanced tumors. AREG is a low affinity EGFR ligand, which is upregulated following treatment with chemotherapeutic drugs. Because AREG depletion retarded growth of xenografted ovarian tumors in mice, we generated a neutralizing monoclonal anti-AREG antibody. The antibody inhibited growth of ovarian cancer xenografts and strongly enhanced chemotherapy efficacy. Taken together, these results raise the possibility that AREG and other low- or high-affinity binders of EGFR might serve as potential targets for cancer therapy.
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http://dx.doi.org/10.1038/onc.2015.93DOI Listing
January 2016

Synaptojanin 2 is a druggable mediator of metastasis and the gene is overexpressed and amplified in breast cancer.

Sci Signal 2015 Jan 20;8(360):ra7. Epub 2015 Jan 20.

Department of Cell Research and Immunology, George Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel.

Amplified HER2, which encodes a member of the epidermal growth factor receptor (EGFR) family, is a target of effective therapies against breast cancer. In search for similarly targetable genomic aberrations, we identified copy number gains in SYNJ2, which encodes the 5'-inositol lipid phosphatase synaptojanin 2, as well as overexpression in a small fraction of human breast tumors. Copy gain and overexpression correlated with shorter patient survival and a low abundance of the tumor suppressor microRNA miR-31. SYNJ2 promoted cell migration and invasion in culture and lung metastasis of breast tumor xenografts in mice. Knocking down SYNJ2 impaired the endocytic recycling of EGFR and the formation of cellular lamellipodia and invadopodia. Screening compound libraries identified SYNJ2-specific inhibitors that prevented cell migration but did not affect the related neural protein SYNJ1, suggesting that SYNJ2 is a potentially druggable target to block cancer cell migration.
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http://dx.doi.org/10.1126/scisignal.2005537DOI Listing
January 2015

Examination of HER3 targeting in cancer using monoclonal antibodies.

Proc Natl Acad Sci U S A 2015 Jan 6;112(3):839-44. Epub 2015 Jan 6.

Departments of Biological Regulation,

The human EGF receptor (HER/EGFR) family of receptor tyrosine kinases serves as a key target for cancer therapy. Specifically, EGFR and HER2 have been repeatedly targeted because of their genetic aberrations in tumors. The therapeutic potential of targeting HER3 has long been underestimated, due to relatively low expression in tumors and impaired kinase activity. Nevertheless, in addition to serving as a dimerization partner of EGFR and HER2, HER3 acts as a key player in tumor cells' ability to acquire resistance to cancer drugs. In this study, we generated several monoclonal antibodies to HER3. Comparisons of their ability to degrade HER3, decrease downstream signaling, and inhibit growth of cultured cells, as well as recruit immune effector cells, selected an antibody that later emerged as the most potent inhibitor of pancreatic cancer cells grown as tumors in animals. Our data predict that anti-HER3 antibodies able to intercept autocrine and stroma-tumor interactions might strongly inhibit tumor growth, in analogy to the mechanism of action of anti-EGFR antibodies routinely used now to treat colorectal cancer patients.
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http://dx.doi.org/10.1073/pnas.1423645112DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4311849PMC
January 2015

Evaluation of knowledge, practices, and possible barriers among healthcare providers regarding medical waste management in Dhaka, Bangladesh.

Med Sci Monit 2014 Dec 9;20:2590-7. Epub 2014 Dec 9.

Department of Healthcare Administration, Nagoya University Graduate School of Medicine, Nagoya, Japan.

Background: Improper handling of medical wastes, which is common in Bangladesh, could adversely affect the hospital environment and community at large, and poses a serious threat to public health. We aimed to assess the knowledge and practices regarding medical waste management (MWM) among healthcare providers (HCPs) and to identify possible barriers related to it.

Material And Methods: A cross-sectional study was carried out during June to September, 2012 including 1 tertiary, 3 secondary, and 3 primary level hospitals in Dhaka division, Bangladesh through 2-stage cluster sampling. Data were collected from 625 HCPs, including 245 medical doctors, 220 nurses, 44 technologists, and 116 cleaning staff who were directly involved in MWM using a self-administered (researcher-administered for cleaning staff), semi-structured questionnaire.

Results: Nearly one-third of medical doctors and nurses and two-thirds of technologists and cleaning staff had inadequate knowledge, and about half of medical doctors (44.0%) and cleaning staff (56.0%) had poor practices. HCPs without prior training on MWM were more likely to have poor practices compared to those who had training. Lack of personal protective equipment, equipment for final disposal, MWM-related staff, proper policy/guideline, and lack of incinerator were identified as the top 5 barriers.

Conclusions: Strengthening and expansion of ongoing educational programs/training is necessary to improve knowledge and practices regarding MWM. The government should take necessary steps and provide financial support to eliminate the possible barriers related to proper MWM.
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http://dx.doi.org/10.12659/MSM.890904DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4266364PMC
December 2014

Differences in structural elements of Bcr-Abl oncoprotein isoforms in Chronic Myelogenous Leukemia.

Bioinformation 2014 19;10(3):108-14. Epub 2014 Mar 19.

Illinois Informatics Institute, University of Illinois, Urbana-Champaign, Illinois, U.S.A.

in silico modeling, using Psipred and ExPASy servers was employed to determine the structural elements of Bcr-Abl oncoprotein (p210(BCR-ABL)) isoforms, b2a2 and b3a2, expressed in Chronic Myelogenous Leukemia (CML). Both these proteins are tyrosine kinases having masses of 210-kDa and differing only by 25 amino acids coded by the b3 exonand an amino acidsubstitution (Glu903Asp). The secondary structure elements of the two proteins show differences in five α-helices and nine β-strands which relates to differences in the SH3, SH2, SH1 and DNA-binding domains. These differences can result in different roles played by the two isoforms in mediating signal transduction during the course of CML.
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http://dx.doi.org/10.6026/97320630010108DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3974235PMC
April 2014

Model of β-Sheet of Muscle Fatty Acid Binding Protein of Locusta migratoria Displays Characteristic Topology.

Bioinformation 2013 27;9(20):1003-9. Epub 2013 Dec 27.

Department of Biochemistry, Faculty of Medicine & Applied Medical Sciences, Northern Border University, Arar-91431, Saudi Arabia.

The β-sheet of muscle fatty acid binding protein of Locusta migratoria (Lm-FABP) was modeled by employing 2-D NMR data and the Rigid Body Assembly method. The model shows the β-sheet to comprise ten β-strands arranged anti-parallel to each other. There is a β-bulge between Ser 13 and Gln 14 which is a difference from the published structure of β-sheet of bovine heart Fatty Acid Binding Protein. Also, a hydrophobic patch consisting of Ile 45, Phe 51, Phe 64 and Phe 66 is present on the surface which is characteristic of most Fatty Acid Binding Proteins. A "gap" is present between βD and βE that provides evidence for the presence of a portal or opening between the polypeptide chains which allows ligand fatty acids to enter the protein cavity and bind to the protein.
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http://dx.doi.org/10.6026/97320630091003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3910355PMC
February 2014

A Structured-based Model for the Decreased Activity of Ala222Val and Glu429Ala Methylenetetrahydrofolate Reductase (MTHFR) Mutants.

Bioinformation 2013 11;9(18):929-36. Epub 2013 Nov 11.

Illinois Informatics Institute, University of Illinois, Urbana-Champaign, Illinois, U.S.A ; Department of Biosciences, COMSATS Institute of Information Technology, Islamabad, Pakistan.

The structure of human Methylenetetrahydrofolate Reductase (MTHFR) is not known either by NMR or by X-ray methods. Phosphorylation seems to play an important role in the functioning of this flavoprotein. MTHFR catalyzes an irreversible reaction in homocysteine metabolism. Phosphorylation decreases the activity of MTHFR by enhancing the sensitivity of the enzyme to SAdenosylmethione. Two common polymorphisms in MTHFR, Ala222Val and Glu429Ala, can result in a number of vascular diseases. Effects of the Glu429Ala polymorphism on the structure of human MTHFR remain undetermined due to limited structural information. Hence, structural models of the MTHFR mutants were constructed using I-TASSER and assessed by PROCHECK, DFIRE and Verify3D tools. A mechanism is further suggested for the decreased activity of the Ala222Val and Glu429Ala mutants due to a decrease in number of serine phosphorylation sites using information gleaned from the molecular models. This provides insights for the understanding of structure-function relationship for MTHFR.
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http://dx.doi.org/10.6026/97320630009929DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3842580PMC
December 2013

Porphyrin derivatives as inhibitors for acetylcholinesterase from Drosophila melanogaster.

Bioinformation 2013 12;9(12):645-9. Epub 2013 Jul 12.

Department of Biochemistry, Faculty of Medicine & Applied Medical Sciences, Northern Border University.

The cure for Alzheimer's disease (AD) is still unknown. According to Cholinergic hypothesis, Alzheimer's disease is caused by the reduced synthesis of the neurotransmitter, Acetylcholine. Regional cerebral blood flow can be increased in patients with Alzheimer's disease by Acetylcholinesterase (AChE) inhibitors. In this regard, Tetraphenylporphinesulfonate (TPPS), 5,10,15,20- Tetrakis (4-sulfonatophenyl) porphyrinato Iron(III) Chloride (FeTPPS) and 5,10,15,20-Tetrakis (4-sulfonatophenyl) porphyrinatoIron(III) nitrosyl Chloride (FeNOTPPS) were investigated as candidate compounds for inhibition of Acteylcholinesterase of Drosophila melanogaster (DmAChE) by use of Molecular Docking. The results show that FeNOTPPS forms the most stable complex with DmAChE.
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http://dx.doi.org/10.6026/97320630009645DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3725007PMC
August 2013

Insight from γC1 protein model for implication in cotton leaf curl disease.

Bioinformation 2013 25;9(9):471-6. Epub 2013 May 25.

Illinois Informatics Institute, University of Illinois, Urbana-Champaign, Illinois, U.S.A.

DNA γ is approximately half of the size of Begomovirus DNA. It encodes a γC1 gene that is conserved in position and size. This gene has the capacity to encode a 13 to 14 kDa protein comprising 118 amino acid residues. It has been shown earlier that γC1 protein is necessary for inducing symptoms of cotton leaf curl disease. The structure for γC1 (CLCuDγ01-Pakistan) is still unknown. Therefore, a model of γC1 (CLCuDγ01-Pakistan) was developed using DoBo and I-TASSER servers followed by validation by PROCHECK and VERIFY 3D servers. The developed model provides an insight in a role for this multifunctional protein in causing Cotton Leaf Curl Disease (CLCuD). A possible function of this protein might be the suppression of RNAsilencing in cotton plants.
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http://dx.doi.org/10.6026/97320630009471DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3705618PMC
July 2013

Binding interactions of porphyrin derivatives with Ca(2+) ATPase of sarcoplasmic reticulum (SERCA1a).

Bioinformation 2013 30;9(8):409-13. Epub 2013 Apr 30.

Department of Biochemistry, Faculty of Medicine and Applied Medical Sciences.

The use of Porphyrin derivatives as photosensitizers in Photodynamic Therapy (PDT) was investigated by means of a molecular docking study. These molecules can bind to intracellular targets such as P-type CaCa(2+) ATPase of sarcoplasmic reticulum (SERCA1a). CAChe software was successfully employed for conducting the docking of Tetraphenylporphinesulfonate(TPPS), 5,10,15,20- Tetrakis (4-sulfonatophenyl) porphyrinato Iron(III) Chloride (FeTPPS) and 5,10,15,20-Tetrakis (4-sulfonatophenyl) porphyrinato Iron(III) nitrosyl Chloride (FeNOTPPS) with CaCa(2+) ATPase from sarcoplasmic reticulum of rabbit. The results show that FeNOTPPS forms the most stable complex with CaCa(2+) ATPase.
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http://dx.doi.org/10.6026/97320630009409DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3670123PMC
June 2013

Autologous transplant in multiple myeloma with an augmented conditioning protocol.

Leuk Lymphoma 2013 Nov 11;54(11):2480-4. Epub 2013 Apr 11.

Department of Bone Marrow Transplantation, Hadassah - Hebrew University Medical Center , Jerusalem , Israel.

We compared the tolerability and anti-myeloma effect of two conditioning regimens for autologous stem cell transplant (auto-SCT) in consecutive groups of patients. Protocol 1 was the earlier, and consisted of the combination of three agents in a sequential manner, including etoposide, thiotepa and melphalan (n = 29), while protocol 2 employed melphalan alone (n = 34). The two groups were comparable (other than younger age in protocol 1). Conditioning with protocol 1 seemed more toxic, as expressed by the higher number of febrile days and higher demand for parenteral nutrition. This was not expressed with longer admission time. With 108 and 60 months' median follow-up, respectively, the median survival in patients treated by protocol 2 (melphalan 200 mg/m(2)) was reached at 59 months, while the median survival was not yet reached in patients treated with protocol 1 (p = 0.039). The time to progression was significantly longer with protocol 1 (median 44 months vs. 17 months with protocol 2, p = 0.033). Confounded by the small number of patients, conditioning with melphalan augmented by etoposide and thiotepa in a sequential manner is slightly more toxic than melphalan alone and may benefit patients with myeloma undergoing auto-SCT.
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http://dx.doi.org/10.3109/10428194.2013.782608DOI Listing
November 2013

α(2)-µ-Globulin fragment (a2-f) from kidneys of male rats.

Bioinformation 2013 6;9(3):145-9. Epub 2013 Feb 6.

Department of Biochemistry, Faculty of Medicine, Northern Border University, Arar-91431, Saudi Arabia.

The structure of α(2)-µ-Globulin fragment (A2-f) is not known.α(2)-µ-Globulin fragment (A2-f) is a 15.5 kDa protein that binds equimolar amount of fatty acids in male rat kidneys. The expression of this protein has been shown to change in response to druginduced and genetic hypertension which suggests that it plays an important role in renal fatty acid metabolism under pathological conditions as well as normal conditions. A2-f has sequence homology with amino acid 28-178 of α(2)-µ-Globulin (A2U) that is synthesized pre-dominantly in the male rat liver and is present in the urine. It is believed that unusual structural features permit A2-f to be targeted to the proximal tubule cell; to escape lysosomal degradation in liver and to enter the cytosol of proximal tubule cells of the kidneys. Homology modeling has been employed to determine the structural elements of this protein and they have been compared with the published structure of A2U. Results suggest differences between the structure of A2-f and its precursor protein A2U.
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http://dx.doi.org/10.6026/97320630009145DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3569602PMC
February 2013

Immunostimulatory and protective effects of Aloe vera against coccidiosis in industrial broiler chickens.

Vet Parasitol 2012 May 25;186(3-4):170-7. Epub 2011 Nov 25.

IImmunoparasitology Laboratory, Department of Parasitology, University of Agriculture, Faisalabad 38040, Pakistan.

This paper reports the immunostimulatory and protective effects of Aloe vera extracts (aqueous and ethanolic) against coccidiosis in industrial broiler chickens. The study was divided into two experiments. Experiment-I was conducted for the evaluation of immunostimulatory activity of A. vera and experiment-II demonstrated the protective efficacy of A. vera extracts against coccidiosis in chickens. Results of the experiment-I revealed significantly higher (p<0.05) lymphoproliferative responses in chickens administered with ethanolic extract of A. vera as compared to those administered with aqueous extract and control group. Microplate haemagglutination assay for humoral response on day 7th and 14th post primary and secondary injections of sheep red blood cells (SRBCs) revealed significantly higher (p<0.05) anti SRBC antibody (total Igs, IgG and IgM) titers in chickens of experimental groups as compared to the control group. None of the extracts, however, demonstrated significant effects on the development of lymphoid organs. Results of experiment-II revealed maximum protection (60%) in chickens administered with aqueous Aloe extract as compared to the ethanolic extract administered chickens (45%). Mean oocysts per gram of droppings in the control group was significantly higher (p<0.05) as compared to the chickens in both the experimental groups. Chickens administered with aqueous Aloe extract showed a minimal mean lesion score (2.3) followed by those administered with ethanolic Aloe extract (2.6) and control chickens (3.05) for caeca, and a similar pattern was observed for intestinal lesion scoring. Further, significantly higher weight gains and antibody titers (p<0.05) were observed in chickens administered with A. vera extracts as compared to those in the control group. It was concluded that A. vera may be a potential and valuable candidate to stimulate the immune responses and can be used successfully as an immunotherapeutic agent against coccidiosis in industrial broiler chickens.
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http://dx.doi.org/10.1016/j.vetpar.2011.11.059DOI Listing
May 2012

Symmetrical peripheral gangrene: a rare presentation of antiphospholipid syndrome.

Intern Emerg Med 2012 May 1;7 Suppl 1:S71-3. Epub 2011 Nov 1.

Department of Surgery, Hamdard Institute of Medical Sciences and Research, Jamia Hamdard University, New Delhi, India.

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http://dx.doi.org/10.1007/s11739-011-0714-4DOI Listing
May 2012

Erosion of repaired exstrophy bladder by a large vesical calculus.

J Indian Assoc Pediatr Surg 2011 Jul;16(3):102-3

Department of Surgery, Hamdard Institute of Medical Sciences and Research, Jamia Hamdard, New Delhi, India.

Formation of stones in repaired exstrophy bladder is common; however, erosion of stone through the repaired bladder and anterior abdominal wall has never been reported. We report one such case of erosion after three years of repair.
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http://dx.doi.org/10.4103/0971-9261.83488DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3160048PMC
July 2011

Perforation into gut by ventriculoperitoneal shunts: A report of two cases and review of the literature.

J Indian Assoc Pediatr Surg 2011 Jan;16(1):31-3

Department of Surgery, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, Uttar Pradesh, India.

We report two cases of gastrointestinal perforation by ventriculoperitoneal (VP) shunts and review the literature on the topic. The time interval between shunt surgery and detection of bowel perforation is minimum in infants and increases with age. Sigmoid and transverse colon followed by stomach are the most frequent sites of gastrointestinal perforations by VP shunts.
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http://dx.doi.org/10.4103/0971-9261.74521DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3047774PMC
January 2011
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