Publications by authors named "Abbas Pardakhti"

4 Publications

  • Page 1 of 1

Cytotoxicity evaluation of curcumin-loaded affibody-decorated liposomes against breast cancerous cell lines.

J Liposome Res 2021 Jun 3;31(2):189-194. Epub 2020 Jul 3.

Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.

Curcumin is known as an effective anticancer herbal medicine but unfortunately, its bioavailability is poor which necessitate efforts for developing more efficient and specific delivery systems. Human epidermal growth factor receptor 2 (HER 2) due to its overexpression in various types of cancers, is demonstrated to be a good candidate as a target for anticancer therapy. In this study, cytotoxicity of curcumin encapsulated in ZHER2:342 Affibody-decorated liposome was investigated against SKBR3 and MCF-7 cancerous cell lines. Curcumin-containing liposomes were prepared from soybeans lecetin and cholesterol by thin-film hydration method. Affibody ZHER2:342 molecules C-terminal cysteine residue were conjugated covalently to the prepared liposomes. Particle size analysis was performed using atomic force microscopy (AFM) and dynamic light scattering (DLS). Curcumin loading was measured using UV-Vis spectrophotometry and cytotoxic activity of curcumin formulations against cancerous cell lines was investigated by MTT assay. Induction of apoptosis was investigated using flow cytometry through Annexin V staining. Particle analysis showed the formation of spherical liposomes with a mean diameter of about 150 nm. Cytotoxic activity of curcumin was improved by its encapsulation in both liposomes and affibody-decorated liposomes. The Annexin V staining indicated the induction of apoptosis by affibody-decorated liposomes in both MCF-7 and SKBR3 cells. Decoration of curcumin-loaded liposomes with affibody ZHER2:342 may improve curcumin apoptotic function independently of HER2 expression level.
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June 2021

Magnetic nickel ferrite nanoparticles: Green synthesis by Urtica and therapeutic effect of frequency magnetic field on creating cytotoxic response in neural cell lines.

Colloids Surf B Biointerfaces 2018 Dec 24;172:244-253. Epub 2018 Aug 24.

Institute of Nano Science and Nano Technology, University of Kashan, P.O. Box 87317-51167, Kashan, Iran. Electronic address:

Despite of great attention concerned on Ni ferrite nanostructures in bioapplications, little is known about the toxicity of these NPs at the cellular and molecular levels. U87 (human primary glioblastoma) and SH-SY5Y (human neuroblastoma) cells treated with various concentration of well-characterized magnetic nickel ferrite nanoparticles, exposed to frequency magnetic field (FMF) and their response was studied. Ferromagnetic nanocrystalline nickel ferrite (NiFeO) powder that characterized by XRD, TEM, SEM, FT-IR, nanosizer, and VSM techniques were prepared by a hydrothermal method in the presence of Urtica plant extract as a green precursor that acts both as reducing and capping agent. Owing to the exceptional properties of green alkalinized agent such as minor toxicity, higher biodegradability, high active surface and environment compatibility, we used the green alkalinized agent (Utrica) to prepared nanostructures for the first time. According to the obtained results, the FMF exposure caused an increase in cell death in neural cell types 48 h after treatment. MNPs indicated dose-dependent cytotoxicity but the amount of cell death per cell in the absence of MFM for SH-SY5Y cells was more than in U87 cells. On the other hand, cell death induced by FMF exposure was observed specifically in SH-SY5Y cells. Nevertheless, it is essential to perform more investigations to find the exact related mechanisms. Imatinib showed dose-dependent antiproliferative effects in all three prostate cancer cell lines.
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December 2018

Efficacy of Pre-Medication with Ibuprofen on Post-Operative Pain after Pulpotomy in Primary Molars.

Iran Endod J 2018 ;13(2):216-220

Department of Pediatric Dentistry, Dental School, Shiraz University of Medical Sciences, Shiraz, Iran.

Introduction: Pain management following dental procedures, particularly pulpotomies and extraction, is of great importance in pediatric dentistry. The aim of this study was to investigate the efficacy of pre-treatment with ibuprofen on post-operative pain following pulpotomy of primary molars.

Methods And Materials: In a split mouth double-blinded randomized clinical trial, 49 children aging between 6-10 years old were given either ibuprofen or a placebo 45 min prior to the treatment. After pulpotomy and placement of a stainless steel crown (SSC), the pain level was evaluated using the Wong-Baker face visual analogue scale for up to 7 days post-treatment. McNemar and Wilcoxon tests were used for data analysis.

Results: Forty-five patients were eligible to participate in this study. Pre-medication with ibuprofen significantly reduced pain during the first 24 h post-treatment (=0.032). However, there was no significant difference in the pain levels between placebo and ibuprofen groups at 48 and 72 h post-treatment (=0.154 and =0.197, respectively). The number of times patients needed analgesics in ibuprofen group was significantly lower compared to that in the placebo group (=0.008).

Conclusion: Pre-medication with ibuprofen resulted in less pain following pulpotomy and SSC placement in primary teeth.
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January 2018

The effect of premedication with ibuprofen and indomethacin on the success of inferior alveolar nerve block for teeth with irreversible pulpitis.

J Endod 2010 Sep 3;36(9):1450-4. Epub 2010 Jul 3.

Oral and Dental Research Center, Kerman University of Medical Sciences, Kerman, Iran.

Introduction: Achieving pulp anesthesia with irreversible pulpitis is difficult. This study evaluated whether nonsteroidal anti-inflammatory drugs assist local anesthesia.

Methods: In a randomized double-blinded clinical trial, 150 patients (50 per group) with irreversible pulpitis were given placebo, 600 mg ibuprofen, or 75 mg indomethacin 1 hour before local anesthesia. Each patient recorded their pain score on a visual analog scale before taking the medication, 15 minutes after anesthesia in response to a cold test, during access cavity preparation and during root canal instrumentation. No or mild pain at any stage was considered a success. Data were analyzed by the chi-square and analysis of variance tests.

Results: Overall success rates for placebo, ibuprofen, and indomethacin were 32%, 78%, and 62%, respectively (p < 0.001). Ibuprofen and indomethacin were significantly better than placebo (p < 0.01). There was no difference between ibuprofen and indomethacin (p = 0.24).

Conclusions: Premedication with ibuprofen and indomethacin significantly increased the success rates of inferior alveolar nerve block anesthesia for teeth with irreversible pulpitis.
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September 2010