Publications by authors named "A Matos-Patrón"

2 Publications

  • Page 1 of 1

Amino acid substitution D222N from fatal influenza infection affects receptor-binding properties of the influenza A(H1N1)pdm09 virus.

Virology 2015 Oct 5;484:15-21. Epub 2015 Jun 5.

Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autonoma de Yucatan, Av. Itzaes #490×59, Centro, C. P., 97000 Merida, Yucatan, Mexico. Electronic address:

We have analyzed the receptor binding profile of A(H1N1)pdm09 recombinant influenza viruses containing the amino acid substitution D222N which has been associated with a fatal case of infection. This mutation was investigated in conjunction with a secondary mutation, S185N. Using human tracheobronchial epithelial cells (HTBE), we found that single mutation D222N affects the binding and replication of the virus during initial stages of infection, with limited but preferred tropism to non-ciliated cells expressing α2,6-SA. However, in conjunction with the S185N change, the (D222N, S185N) virus shows a remarkable increase in binding and replication efficiency, with tropism for both ciliated and non-ciliated cells. Glycan microarray analysis demonstrated correlation between the binding profile and the cell tropism observed in the HTBE cells. These findings suggest that viruses with D222N required compensatory mutations such as S185N to maintain viral fitness, and in combination, affect the pathogenicity of the virus and the clinical outcome.
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http://dx.doi.org/10.1016/j.virol.2015.05.012DOI Listing
October 2015

Hemagglutinin variants of influenza A(H1N1)pdm09 virus with reduced affinity for sialic acid receptors.

Arch Virol 2014 May 3;159(5):1207-11. Epub 2013 Dec 3.

Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autonoma de Yucatan, Av. Itzaes #490 x 59, Centro, C. P. 97000, Merida, Yucatan, Mexico,

In influenza A H1 virus, amino acids at position 190 and 225 of HA affect replication and transmission. In this study, we show that the mutation D190Y in the HA of influenza AH1N1pdm09 virus reduces the affinity of the virus for sialic acid receptors expressed at the surface of red blood cells from different species without affecting virus replication in MDCK cells.
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http://dx.doi.org/10.1007/s00705-013-1934-xDOI Listing
May 2014