Publications by authors named "A Adrover"

34 Publications

Brownian Sieving Effect for Boosting the Performance of Microcapillary Hydrodynamic Chromatography. Proof of Concept.

Anal Chem 2021 05 23;93(17):6808-6816. Epub 2021 Apr 23.

Dipartimento di Ingegneria Chimica Materiali Ambiente, Sapienza Università di Roma, Via Eudossiana 18, Roma 00184, Italy.

Microcapillary hydrodynamic chromatography (MHDC) is a well-established technique for the size-based separation of suspensions and colloids, where the characteristic size of the dispersed phase ranges from tens of nanometers to micrometers. It is based on hindrance effects which prevent relatively large particles from experiencing the low velocity region near the walls of a pressure-driven laminar flow through an empty microchannel. An improved device design is here proposed, where the relative extent of the low velocity region is made tunable by exploiting a two-channel annular geometry. The geometry is designed so that the core and the annular channel are characterized by different average flow velocities when subject to one and the same pressure drop. The channels communicate through openings of assigned cut-off length, say . As they move downstream the channel, particles of size bigger than are confined to the core region, whereas smaller particles can diffuse through the openings and spread throughout the entire cross section, therein attaining a spatially uniform distribution. By using a classical excluded-volume approach for modeling particle transport, we perform Lagrangian-stochastic simulations of particle dynamics and compare the separation performance of the two-channel and the standard (single-channel) MHDC. Results suggest that a quantitative (up to thirtyfold) performance enhancement can be obtained at operating conditions and values of the transport parameters commonly encountered in practical implementations of MHDC. The separation principle can readily be extended to a multistage geometry when the efficient fractionation of an arbitrary size distribution of the suspension is sought.
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http://dx.doi.org/10.1021/acs.analchem.1c00780DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8253478PMC
May 2021

Swelling and Drug Release in Polymers through the Theory of Poisson-Kac Stochastic Processes.

Gels 2021 Mar 22;7(1). Epub 2021 Mar 22.

Dipartimento di Ingegneria Chimica, Materiali e Ambiente, Sapienza Università di Roma, via Eudossiana 18, 00184 Roma, Italy.

Experiments on swelling and solute transport in polymeric systems clearly indicate that the classical parabolic models fail to predict typical non-Fickian features of sorption kinetics. The formulation of moving-boundary transport models for solvent penetration and drug release in swelling polymeric systems is addressed hereby employing the theory of Poisson-Kac stochastic processes possessing finite propagation velocity. The hyperbolic continuous equations deriving from Poisson-Kac processes are extended to include the description of the temporal evolution of both the Glass-Gel and the Gel-Solvent interfaces. The influence of polymer relaxation time on sorption curves and drug release kinetics is addressed in detail.
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http://dx.doi.org/10.3390/gels7010032DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8006012PMC
March 2021

Integrated Multiomics Reveals Glucose Use Reprogramming and Identifies a Novel Hexokinase in Alcoholic Hepatitis.

Gastroenterology 2021 04 10;160(5):1725-1740.e2. Epub 2020 Dec 10.

Daniel Baugh Institute, Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, Philadelphia, Pennsylvania.

Background & Aims: We recently showed that alcoholic hepatitis (AH) is characterized by dedifferentiation of hepatocytes and loss of mature functions. Glucose metabolism is tightly regulated in healthy hepatocytes. We hypothesize that AH may lead to metabolic reprogramming of the liver, including dysregulation of glucose metabolism.

Methods: We performed integrated metabolomic and transcriptomic analyses of liver tissue from patients with AH or alcoholic cirrhosis or normal liver tissue from hepatic resection. Focused analyses of chromatin immunoprecipitation coupled to DNA sequencing was performed. Functional in vitro studies were performed in primary rat and human hepatocytes and HepG2 cells.

Results: Patients with AH exhibited specific changes in the levels of intermediates of glycolysis/gluconeogenesis, the tricarboxylic acid cycle, and monosaccharide and disaccharide metabolism. Integrated analysis of the transcriptome and metabolome showed the used of alternate energetic pathways, metabolite sinks and bottlenecks, and dysregulated glucose storage in patients with AH. Among genes involved in glucose metabolism, hexokinase domain containing 1 (HKDC1) was identified as the most up-regulated kinase in patients with AH. Histone active promoter and enhancer markers were increased in the HKDC1 genomic region. High HKDC1 levels were associated with the development of acute kidney injury and decreased survival. Increased HKDC1 activity contributed to the accumulation of glucose-6-P and glycogen in primary rat hepatocytes.

Conclusions: Altered metabolite levels and messenger RNA expression of metabolic enzymes suggest the existence of extensive reprogramming of glucose metabolism in AH. Increased HKDC1 expression may contribute to dysregulated glucose metabolism and represents a novel biomarker and therapeutic target for AH.
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http://dx.doi.org/10.1053/j.gastro.2020.12.008DOI Listing
April 2021

Intraoperative Hemostatic Management of a Cardiohepatic Transplant With Rotational Thromboelastometry: A Case Report.

A A Pract 2020 Dec;14(14):e01358

From the Department of Anesthesia, Hospital Italiano de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina.

Cardiohepatic transplantation represents a double hemostatic challenge. Given the absence of specific guidelines and current evidence, we designed a 5-step approach based on rotational thromboelastometry (ROTEM). A 60-year-old male patient with cirrhosis and myocarditis underwent a 9-hour transplantation. Bleeding occurred after weaning from extracorporeal circulation. Evidence of reduced clot strength triggered fibrinogen and platelet replacement therapy. During liver transplant, only hemoglobin optimization was necessary. In conclusion, hemostatic management protocols for cardiohepatic transplants should consider the specific coagulopathy mechanisms underlying each surgical phase. Because whole blood testing is essential for their diagnosis, we recommend using ROTEM for optimal coagulation management.
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http://dx.doi.org/10.1213/XAA.0000000000001358DOI Listing
December 2020

A Non-Isothermal Moving-Boundary Model for Continuous and Intermittent Drying of Pears.

Foods 2020 Oct 30;9(11). Epub 2020 Oct 30.

Dipartimento di Ingegneria Chimica, Materiali e Ambiente, Sapienza Università di Roma, via Eudossiana 18, 00184 Roma, Italy.

A non-isothermal moving-boundary model for food dehydration, accounting for shrinkage and thermal effects, is proposed and applied to the analysis of intermittent dehydration in which air temperature, relative humidity, and velocity vary cyclically in time. The convection-diffusion heat transport equation, accounting for heat transfer, water evaporation, and shrinkage at the sample surface, is coupled to the convection-diffusion water transport equation. Volume shrinkage is not superimposed but predicted by the model through the introduction of a point-wise shrinkage velocity. Experimental dehydration curves, in continuous and intermittent conditions, are accurately predicted by the model with an effective water diffusivity Deff(T) that depends exclusively on the local temperature. The non-isothermal model is successfully applied to the large set of experimental data of continuous and intermittent drying of pears.
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http://dx.doi.org/10.3390/foods9111577DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7692062PMC
October 2020
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