Publications by authors named "Paulina"

13 Publications

  • Page 1 of 1

[Reemergence of Pediatric Tuberculosis and associated aspects: Metropolitan Region of Chile, 2005 to 2018].

Andes Pediatr 2021 Apr;92(2):202-209

Hospital San Borja Arriarán, Santiago, Chile.

Objective: To describe the socio-epidemiological characteristics of Pediatric Tuberculosis (TB) ca ses, and aspects associated with its incidence, from 2005 to 2018, in North, Central, and South Metro politan Health Services of Chile.

Patients And Method: Descriptive study with an ecological time se ries. We studied the incidence of annual global TB and according to age, sex, location of tuberculosis, bacteriological confirmation, human immunodeficiency virus (HIV) co-infection, country of origin, and effectiveness of anti-tuberculosis therapy. In addition, a correlation analysis was made between the incidence rate and the proportion of foreign cases.

Results: Between 2005 and 2018, 160 cases of TB were reported in children under 15, with a median age of 8 years, and 55% male. 56.2% corres ponded to Pulmonary TB, of which 65.6% were bacteriologically confirmed. The incidence rate in creased from 0.5 cases x 100,000 inhabitants in 2010, to 4.9 cases x 100,000 inhabitants in 2018. This last year concentrated 20.6% of the cases that occurred in 14 years of observation. The proportion of cases in foreign minors is still lower compared with the cases in Chilean children. A 91.3% success rate, 6.5% of cases transferred to other Services, and 2 deaths were also observed.

Conclusion: The results of this study show a serious epidemiological reemergence of TB. We recommend that control and eradication policies consider strategies focused on pediatric patients, aiming at actively screening for new cases and more efficient contact tracing.
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http://dx.doi.org/10.32641/andespediatr.v92i2.2141DOI Listing
April 2021

Assessment of persistent indoor VOCs inside public transport during winter season.

Chemosphere 2021 Jan 28;263:128127. Epub 2020 Aug 28.

CIATEC AC, Centro de Innovación Aplicada en Tecnologías Competitivas, León México, Omega No. 201 Col. Industrial Delta C.P, 37545, León, Gto, Mexico. Electronic address:

The present work intends to analyze the pollution level at the indoor environments of the public transport units of León Guanajuato, Mexico during winter season. An identification and quantification of persistent organic pollutants were carried out within three of the principal bus lines of the city in order to determine their possible origin, the differences in the levels of contamination between routes, and the potential risk to the health of the users, these analyses were carried out with different statistical techniques (ANOVA, PCA, and correlation network maps). Fourteen different organic compounds were identified as persistent pollutants. Although toluene and hexane were the compounds that were detected at the highest concentrations (average of 86.52 ± 56.1 μg m and 183.33 ± 10.7 μg m, respectively), the correlation analysis showed that xylene, styrene, and ethylbenzene were the compounds that were mostly related to the other compounds identified as persistent. Otherwise, the statistical analysis of the concentration of these pollutants allowed to establish the fuel combustion vapors as the main source of these compounds. In the same way, the potential exposition health risk to the users were calculated in accordance to the Environmental Protection Agency of United States on those commuters grouped as students and workers. This analysis shown that the xylenes are the most representative organic pollutant in this particulate indoor spaces, and is the one with potential to generate a greater risk to the health of the bus-users, this without demising the potential danger of other pollutants.
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http://dx.doi.org/10.1016/j.chemosphere.2020.128127DOI Listing
January 2021

Giant vaginal stone as a complication of long-term urethrovaginal fistula: A case report.

Urol Case Rep 2021 Jan 11;34:101491. Epub 2020 Nov 11.

Department of Urology, Faculty of Medicine Padjadjaran University, Hasan Sadikin Hospital Bandung, Indonesia.

Case Presentation: A case of a 57 years old woman came with the chief complaint of urinary incontinence since 20 years ago. After labor, she complained of urinary incontinence and left untreated. Physical examination and urethrocystoscopy revealed vaginal stone sized 90 × 70 mm and urethrovaginal fistula. We performed hysterectomy and vaginal stone removal, continued with fistula closure and vaginal repair. Up to 2 months follow up, no sign of urinary leakage and incontinence was found.

Conclusion: Vaginal stone is a rare Case that might be present in a case of long term urethrovaginal fistula with neglected contraceptive device.
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http://dx.doi.org/10.1016/j.eucr.2020.101491DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7701185PMC
January 2021

Undifferentiated embryonal sarcoma of the liver in adult patient: A report of two cases.

Ann Hepatobiliary Pancreat Surg 2018 Aug 31;22(3):269-273. Epub 2018 Aug 31.

Surgery Department, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico.

Primary sarcomas of the liver are unusual neoplasms developing in adults. They constitute a heterogeneous group of neoplasms including undifferentiated embryonal sarcoma. Patients usually present with an abdominal mass and abdominal pain. Case 1: A 53-year-old woman presented with abdominal pain. Computed tomography showed an occupying mass in the right lobule and an intra-auricular multi-lobulated mass suggestive of a secondary deposit. Biopsy of the hepatic lesion revealed undifferentiated embryonal sarcoma. Despite radiotherapy and supportive measures, her overall status progressively worsened until cardiac arrest. Case 2: A 41-year-old woman presented with hepatomegaly. Abdominal imaging showed cystic lesions in the right hepatic lobule with multiple septae. The patient underwent extended right hepatectomy and a histopathological study reported high-grade undifferentiated embryonal sarcoma. Two years after surgery, a new cystic lesion in the surgical site was recorded and chemotherapy was scheduled. The lesion remained stable for three years when disease progression was observed and second-line chemotherapy was initiated. Although undifferentiated embryonal sarcoma of the liver has poor prognosis, early diagnosis is essential to increase the chances of survival. Currently, surgical resection and chemotherapy are the primary treatment modalities.
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http://dx.doi.org/10.14701/ahbps.2018.22.3.269DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6125275PMC
August 2018

Biomechanical and morphological stability of acellular scaffolds for tissue-engineered heart valves depends on different storage conditions.

J Mater Sci Mater Med 2018 Jul 3;29(7):106. Epub 2018 Jul 3.

Heart Prosthesis Institute, Bioengineering Laboratory, Wolnosci 345A, 41-800, Zabrze, Poland.

Currently available bioprosthetic heart valves have been successfully used clinically; however, they have several limitations. Alternatively, tissue-engineering techniques can be used. However, there are limited data concerning the impact of storage conditions of scaffolds on their biomechanics and morphology. The aim of this study was to determine the effect of different storage conditions on the biomechanics and morphology of pulmonary valve dedicated for the acellular scaffold preparation to achieve optimal conditions to obtain stable heart valve prostheses. Scaffold can then be used for the construction of tissue-engineered heart valve, for this reason evaluation of these parameters can determine the success of the clinical application this type of bioprosthesis. Pulmonary heart valves were collected from adult porcines. Materials were divided into five groups depending on the storage conditions. Biomechanical tests were performed, both the static tensile test, and examination of viscoelastic properties. Extracellular matrix morphology was evaluated using transmission electron microscopy and immunohistochemistry. Tissue stored at 4 °C exhibited a higher modulus of elasticity than the control (native) and fresh acellular, which indicated the stiffening of the tissue and changes of the viscoelastic properties. Such changes were not observed in the radial direction. Percent strain was not significantly different in the study groups. The storage conditions affected the acellularization efficiency and tissue morphology. To the best of our knowledge, this study is the first that attributes the mechanical properties of pulmonary valve tissue to the biomechanical changes in the collagen network due to different storage conditions. Storage conditions of scaffolds for tissue-engineered heart valves may have a significant impact on the haemodynamic and clinical effects of the used bioprostheses.
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http://dx.doi.org/10.1007/s10856-018-6106-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6028870PMC
July 2018

[The effect of low frequency electrical stimulation on bone tissue regeneration].

Patol Fiziol Eksp Ter 2015 Jul-Sep(3):94-9

The study was performed on 30 male rats of Wistar line (weight 330-360 g, age 3.5 months).In an experimental model of damage to the femur bone in the hip joint studied the effect of low frequency electrical stimulation of the damaged area on the rate of regeneration of bone. The animals were divided into two groups. Control (15 rats) and experienced (15 rats). In the experimental animals underwent stimulation of the injury site for 5 min daily for 7 days, 14 days and 21 days. Stimulation was carried out using a device "Osteon-1" generating a mixed signal of two voltage pulse of varying duty cycle, one of which is modulated to a higher frequency. Signals were not synchronized with respect to each other, unipolar with varying frequencies and amplitudes. The obtained results show the effectiveness of the electrical stimulation currents of low frequency in the restoration of bone tissue after damage. Morphological studies showed that electrical stimulation to accelerate the regeneration of damaged bone at all stages of the study (7, 14, 21 day), causes a more pronounced integration of newly formed bone with the old intact bone and promote the formation of more powerful periosteal calluses in comparison with the control.
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February 2016

Detection of pAkt protein in imprint cytology of invasive breast cancer: Correlation with HER2/neu, hormone receptors, and other clinicopathological variables.

Cytojournal 2015 25;12. Epub 2015 Mar 25.

Address: Department of Cytopathology, Aretaieion University Hospital, Athens, Greece, Europe.

Purpose: Akt is a serine/threonine protein kinase and has emerged as a crucial regulator of widely divergent cellular processes, including apoptosis, proliferation, differentiation, and metabolism. Activation of Akt/protein kinase B has been positively associated with human epidermal growth-factor receptor 2 (HER2)/neu overexpression in breast carcinoma and a worse outcome among endocrine treated patients. The Akt signaling pathway currently attracts considerable attention as a new target for effective therapeutic strategies. We therefore investigated the relationship between activation of Akt and clinicopathologic variables including hormone receptor and HER2/neu status.

Methods: Archival tumor tissues from 100 patients with invasive breast carcinoma were analyzed by immunocytochemistry. This study describes the results of immunocytochemical pAkt expression in breast carcinoma imprints, prepared from cut surfaces of freshly removed tumors. Both nuclear and cytoplasmic expressions were evaluated for pAkt.

Results: Nuclear and cytoplasmic positive scores of 72% (72/100) and 42% (42/100), respectively, were found. Coexistence of nuclear and cytoplasmic staining was observed in 32 cases (32/100). Nuclear positive staining correlated with HER2/neu overexpression (P = 0.043) and was significantly associated with positive involvement of axillary lymph nodes (P = 0.013). No correlation was found between cytoplasmic pAkt rate and clinicopathological parameters, estrogen receptor, progesterone receptor or HER2/neu expression.

Conclusions: pAkt expression can be evaluated in cytological material and may add valuable information to current prognostic models for breast cancer. pAkt overexpression appears to be linked with potentially aggressive tumor phenotype in invasive breast carcinoma.
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http://dx.doi.org/10.4103/1742-6413.153965DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4382762PMC
April 2015

Intraocular lens subluxation in marfan syndrome.

Open Ophthalmol J 2014 19;8:48-50. Epub 2014 Sep 19.

Department of Cornea and Refractive Surgery Institute of Ophthalmology, Fundación Conde de Valenciana, Mexico City, Mexico.

Purpose: Ectopia lentis (EL) is a major criteria for the diagnosis of Marfan syndrome, it may vary from an asymptomatic mild displacement to a significant subluxation that places the equator of the lens in the pupillary axis. The purpose of this work is to present the case of a patient with Marfan syndrome who received treatment for subluxation at our institution.

Case Report: A 51-year-old female diagnosed with Marfan syndrome presented to the emergency department with bilateral eye redness, foreign body sensation and crusting around the eyes on awakening. She had the following history of cardiac and ophthalmologic complications, including: 1. Lens subluxation 2. High myopia 3. Aortic root dilation, 4. Mitral valve prolapse and 5. Tricuspid insufficiency.

Conclusion: The ophthalmological management of Marfan patients is challenging and periodical follow-up is needed. Surgical versus conservative management is controversial, each case needs to be evaluated individually to analyze the risks and benefits of the procedures.
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http://dx.doi.org/10.2174/1874364101408010048DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4181168PMC
October 2014

EGFR activating mutations detected by different PCR techniques in Caucasian NSCLC patients with CNS metastases: short report.

Clin Exp Metastasis 2013 Dec 27;30(8):1063-71. Epub 2013 Jul 27.

Pneumonology, Oncology and Allergology Department, Medical University of Lublin, Jaczewskiego 8, 20-954, Lublin, Poland,

EGFR mutation testing has become an essential determination to decide treatment options for NSCLC. The mutation analysis is often conducted in samples with low percentage of tumour cells from primary tumour biopsies. There is very little evidence that samples from metastatic tissues are suitable for EGFR testing. We had evaluated the frequency of EGFR mutations with three highly sensitive PCR techniques in formalin-fixed, paraffin-embedded samples of 143 NSCLC patients with central nervous system (CNS) metastases. 32 corresponding primary tumours were also examined. We used PCR followed by DNA fragments length analysis (FLA), ASP-PCR and PNA-LNA PCR clamp techniques. We found 9 (6.29 %) EGFR gene mutations in CNS samples: 3 (2.1 %) in exon 19 and 6 (4.2 %) in exon 21. The full concordance between CNS metastases and primary tumour samples was observed. PCR followed by DNA-FLA and PNA-LNA PCR clamp were sensitive enough to detect exon 19 deletions. Two mutations in exon 21 were detected by ASP-PCR only, one L858R substitution was detected only by PNA-LNA PCR clamp. With respect to sensitivity, PCR followed by DNA-FLA achieved a level of detection of at least 10 % of mutated DNA for exon 19 deletion, as for ASP-PCR it was at least 5 % of mutated DNA for L858R substitution. Higher sensitivity of 1 % of mutated DNA was achieved by PNA-LNA PCR clamp technique for both mutations. The use of different methodological techniques authenticates the negative result of molecular tests.
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http://dx.doi.org/10.1007/s10585-013-9603-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3841581PMC
December 2013

Virtual reality based therapy for post operative rehabilitation of children with cerebral palsy.

Work 2012 ;41 Suppl 1:3612-5

RECOUP Neuromusculoskeletal Rehabilitation Centre, Bangalore, India.

Virtual reality is the use of interactive replication created with computer hardware and software to impart users with opportunities to engage in environments that appear to feel similar to real world objects and events. The commonest rehabilitation program of cerebral palsy children involves stretching, strengthening, mobilization and various other activities, whereas the use of virtual reality based training (VRBT) for rehabilitation of cerebral palsy is not common. To understand the effect of VRBT a study was formulated. Twenty nine subjects participated (study group--14 and control group--15). Outcome measures were MACS, PBS, level of participation, motivation, cooperation and satisfaction of the child. Results revealed that balance and manual ability were significantly improved in both the groups (Balance: study: t-2.28, p<0.05; control: t-3.5, p<0.01; Manual ability: study: t-5.58, p<0.001; control: t-7.06, p<0.001). PBS had significantly greater improvement in the study group (t-t-2.02, p<0.05). Level of participation, motivation, cooperation and satisfaction of the child were also reported to be significantly higher among the study group as compared with control group. To the author's best knowledge, this is the first study on using the VR-based therapy for the postoperative rehabilitation of children with CP which need further elaboration with larger sample size.
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http://dx.doi.org/10.3233/WOR-2012-0667-3612DOI Listing
February 2014

Oyster hemocytes express a proline-rich peptide displaying synergistic antimicrobial activity with a defensin.

Mol Immunol 2009 Feb 28;46(4):516-22. Epub 2008 Oct 28.

Ifremer, CNRS, Université de Montpellier II, UMR 5119 "Ecosystèmes Lagunaires", Place Eugène Bataillon, CC80, 34095 Montpellier Cedex 5, France.

A cDNA sequence that encodes a 61-amino acid polypeptide precursor with homologies to proline-rich antimicrobial peptides (AMPs) was identified in the oyster Crassostrea gigas. After release of a hydrophobic signal peptide, the resulting 37-amino acid peptide, Cg-Prp, is composed of an acidic region and a cationic proline-rich region. To evaluate the biological properties of Cg-Prp, multiple proline-rich peptides corresponding to putative processing of the full-length Cg-Prp were synthesized. A limited antimicrobial activity was observed for two of them, which also showed strong synergistic antimicrobial activity with Cg-Def, a defensin from C. gigas. To our knowledge, this is the first evidence of synergy between a defensin and another AMP in an invertebrate. By in situ hybridization, the expression of Cg-prp was found to be restricted to hemocytes and induced following bacterial challenge. Cg-prp transcripts were also detected in hemocytes infiltrating mantle, where Cg-Def is expressed. Additionally, by immunocytochemistry, we showed that Cg-Prp or one of its variants is present in some hemocytes together with defensins. In conclusion, we described here the first proline-rich AMP from mollusk. From our study, it is likely to provide a first line of defense against bacterial invasion by acting through synergy with defensins.
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http://dx.doi.org/10.1016/j.molimm.2008.07.021DOI Listing
February 2009

Inhibition of inducible nitric oxide synthase and cyclooxygenase-2 expression by flavonoids isolated from Tanacetum microphyllum.

Int Immunopharmacol 2006 Nov 10;6(11):1723-8. Epub 2006 Aug 10.

Department of Pharmacology, Faculty of Pharmacy, University Complutense, Ciudad Universitaria s/n, 28040, Madrid, Spain.

Plant flavonoids show anti-inflammatory activity both in vitro and in vivo. Some flavonoids have been reported previously to inhibit nitric oxide (NO) and prostaglandin E2 (PGE2) production by suppressing inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression. The present study focuses on the effect of various naturally occurring flavonoids (santin, ermanin, centaureidin and 5,3'-dihydroxy-4'-methoxy-7-methoxycarbonylflavonol) on modulation of lipopolysaccharide (LPS)-induced iNOS and COX-2 expression in RAW 264.7 cells. Western blotting showed that all flavonoids suppressed the induction of both iNOS and COX-2. Ermanin and 5,3'-dihydroxy-4'-methoxy-7-methoxycarbonylflavonol were the most potent inhibitors. This study suggests that inhibition of iNOS and COX-2 expression by flavonoids may be one of the mechanisms responsible for their anti-inflammatory effects, and that they may be potential agents for use in the treatment of inflammatory diseases.
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http://dx.doi.org/10.1016/j.intimp.2006.07.012DOI Listing
November 2006

A department of personal and family medicine.

Med J Aust 1975 Sep;2(13):504-9

There has been increasing, worldwide dissatisfaction with the relevance of medical education to health care. Recently, a special task group set up by The Royal Australian College of General Practitioners made a study of how a department of personal and family medicine could participate in the education of the medical student. The task was to present first steps in a research and development programme for education of health personnel to work in and near the family setting. Two academics, two nurse educators, two general practitioners, one specialist surgeon involved in medical education and a fifth-year medical student made up the group. It worked at the task during a six-day live-in teaching and leadership seminar at Leura, New South Wales, in February, 1973.
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http://dx.doi.org/10.5694/j.1326-5377.1975.tb106022.xDOI Listing
September 1975
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