Publications by authors named "Elvina"

6 Publications

  • Page 1 of 1

Fibrinolytic characteristics of G8 isolated from natto.

Biosci Microbiota Food Health 2021 20;40(3):144-149. Epub 2021 Feb 20.

Department of Biology, Faculty of Science & Technology, Universitas Pelita Harapan, Tangerang 15811, Banten, Indonesia.

Due to the high prevalence of vascular obstructive diseases, discovering potent, safe, and affordable fibrinolytic agents is of importance. There is particular interest concerning the use of functional foods that have a fibrinolytic activity, such as natto, a Japanese fermented soy-based product made with (natto) strain BEST195. We recently isolated another bacterial strain from natto commercialized in Indonesia, G8, which has proven to exert fibrinolytic activity. Herein, a further characterization of G8 was assessed through a comparison with commercialized nattokinase, the major fibrinolytic enzyme of , by utilizing various fibrinolytic assays, namely whole blood clot lysis, euglobulin clot lysis, the fibrin plate method, and zymography. Both nattokinase and G8 were able to dissolve both whole blood and euglobulin clots. Furthermore, both nattokinase and G8 were able to lyse blood clots, presumably due to their ability to directly lyse fibrin. Finally, a crude extract of G8 displayed six zymogram bands of approximately 42.0, 35.5, 30.8, 26.7, 20.0, and 13.7 kDa, with the strongest activity observed at 20.0 kDa. This indicates that G8 contained several fibrinolytic enzymes, which might have comprised nattokinase and other fibrinolytic enzymes. In summary, we demonstrated that a crude extract of G8 has potent fibrinolytic activity and that the activity was mediated by various fibrinolytic enzymes.
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February 2021

The acceptability of mass administrations of anti-malarial drugs as part of targeted malaria elimination in villages along the Thai-Myanmar border.

Malar J 2016 09 27;15(1):494. Epub 2016 Sep 27.

Shoklo Malaria Research Unit, Mae Sot, Thailand.

Background: A targeted malaria elimination project, including mass drug administrations (MDA) of dihydroartemisinin/piperaquine plus a single low dose primaquine is underway in villages along the Thailand Myanmar border. The intervention has multiple components but the success of the project will depend on the participation of the entire communities. Quantitative surveys were conducted to study reasons for participation or non-participation in the campaign with the aim to identify factors associated with the acceptance and participation in the mass drug administrations.

Methods: The household heads in four study villages in which MDAs had taken place previously were interviewed between January 2014 and July 2015.

Results: 174/378 respondents (46 %) completed three rounds of three drug doses each, 313/378 (83 %) took at least three consecutive doses and 56/378 (15 %) did not participate at all in the MDA. The respondents from the two villages (KNH and TPN) were much more likely to participate in the MDA than respondents from the other two villages (HKT and TOT). The more compliant villages KNH and TPN had both an appearance of cohesive communities with similar demographic and ethnic backgrounds. By contrast the villages with low participation were unique. One village was fragmented following years of armed conflict and many respondents gave little inclination to cooperate with outsiders. The other village with low MDA coverage was characterised by a high percentage of short-term residents with little interest in community interventions. A universal reason for non-participation in the MDA applicable to all villages was an inadequate understanding of the intervention.

Conclusions: It is unlikely that community engagement can unite fragmented communities in participating in an intervention, which benefits the community. Understanding the purpose and the reasons underlying the intervention is an important pre-condition for participation. In the absence of direct benefits and a complete understanding of the indirect benefits trust in the investigators is critical for participation.
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September 2016


J Philipp Med Assoc 1964 Feb;40:122-35

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February 1964