Publications by authors named "Deepshikha"

9 Publications

  • Page 1 of 1

Homology Modeling and Protein Interaction Map of CHRNA7 Neurogenesis Protein.

Ann Neurosci 2017 Jul 24;24(3):173-179. Epub 2017 Jul 24.

AMITY Institute of Biotechnology, AMITY University, Lucknow, India.

CHRNA7 is a neurodevelopmental protein involved in differentiation and neurogenesis, which is also named as nicotinic acetylcholine receptors, cholinergic receptor, nicotinic, alpha 7 (neuronal). The protein encoded by this gene forms a homo-oligomeric channel. It is a major component of brain nicotinic receptors displays that are blocked by and sensitive to alpha-bungarotoxin. Studies reports involvement of CHRNA7 protein in different neurological diseases. Non-availability of 3-dimensional (3D) structure leads the study toward structure 3D prediction along with its interaction analysis. The current paper is focused on the structure prediction through homology modeling of CHRNA7 along with binding site prediction using Schrödinger software suite. In continuation of the study, protein-protein interaction analysis is carried out by using string database. Tertiary structure along with binding sites was obtained, and visualized CHRAN7 protein have interaction with CHRNA protein family along with JAK2, AKT1, PICK1 protein that are involved in neurological disease. Structure formation analysis is an important aspect of proteomics studies. Hence, this predicted structure can be used for further advance studies and drug designing. Protein interaction analysis shows that CHRNA7 protein also interact with AKT1 protein which regulate neuronal differentiation and development, that signifies the role of CHRNA7 protein in neurological diseases.
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http://dx.doi.org/10.1159/000477155DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5566677PMC
July 2017

Lower Plasma Soluble Transferrin Receptor Range in Healthy Indian Pediatric Cohort as Compared to Asian and Western Data.

Indian J Hematol Blood Transfus 2017 Sep 19;33(3):405-407. Epub 2016 Aug 19.

Department of Pediatrics, Advanced Pediatrics Centre, Post Graduate Institute of Medical Education and Research, Chandigarh, 160012 India.

Soluble serum transferrin receptor is derived from erythroid transferrin receptor expressed on surface of developing erythroid cells. It can be detected in blood using sensitive ELISA methodology and blood levels reflect physiological iron dependent erythropoiesis state in bone marrow. Normal adult levels vary from 2 to 5 mg/l. However, pediatric studies are few and describe normal ranges to the tune of 1.0-3.0 mg/l, which are relatively lower than that of adults. In present study 40 healthy children (2-12 years) were evaluated to establish normal soluble transferrin receptor range. The mean transferrin receptor levels were 0.39 mg/l with a range of 0.17-2.1 mg/l. The levels were low as compared to mean levels described in other studies from West and our country (4.39 and 2.0 mg/l respectively). Since, no internationally standard method for reporting and testing for transferrin receptor levels are yet available, hence it is imperative to establish normal control ranges in different population cohorts, especially in pediatric age group, to better interpret their levels in diagnostic context.
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http://dx.doi.org/10.1007/s12288-016-0716-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5544626PMC
September 2017

An enzyme free Vitamin C augmented sensing with different ZnO morphologies on SnO2/F transparent glass electrode: A comparative study.

Mater Sci Eng C Mater Biol Appl 2016 Dec 7;69:769-79. Epub 2016 Jul 7.

Amity Institute of Nanotechnology, Amity University, Noida, UP, India. Electronic address:

Three types of Zinc oxide (ZnO) nanostructures viz. ZnO nanocrystals (ZnONCs), ZnO nanoparticles (ZnONPs) and ZnO nanobelts (ZnONBs) were synthesized and characterized by UV-Vis, FTIR and SEM. A comparison of signal amplification by these ZnO nanostructures as judged by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and Linear Sweep Voltammetry (LSV) revealed that ZnONCs are better sensing interface for electrochemical detection. When these ZnO nanostructure were compared electrochemically for sensing Vitamin C, ZnONC's sensor outperformed the ZnONP and ZnONB sensor and previously reported sensors. The ZnONCs/MB/FTO electrode showed a wide linear sensing range (0.001μM to 4000μM), low detection limit (0.0001μM), a small response time (5s) and a storage stability of 6months. To the best of our knowledge, this elevated sensitivity and remarkable stability for electrochemical Vitamin C detection using ZnONC's have not been reported so far.
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http://dx.doi.org/10.1016/j.msec.2016.07.012DOI Listing
December 2016

A study on morbidity profile and associated risk factors in a rural area of dehradun.

J Clin Diagn Res 2014 Aug 20;8(8):JC01-4. Epub 2014 Aug 20.

Assistant Professor, Department of Community Medicine, HIMS, SRH University , Dehradun, India .

Background: First few years of life is the most crucial period of life as this age is known for accelerated growth and development, warranting regular monitoring. During this period about 40% of physical growth and 80% of mental development occurs. Any adverse influences during this period may result in severe limitations in their development.

Methods: A cross-sectional study was conducted in the field practice area of Rural Health Training Centre. Mothers of children between 0-3 years of age were enquired about the morbidity in the past two months.

Results: Diarrhea (47.9%) followed by ARI (22.21%), Pica (13%) and Worm Infestation (9.21%) were the commonest morbidities found in the study population. Morbidities were found to be common in males as compared to females. Amongst the nutritional deficiencies anaemia & Vitamin B deficiencies were the commonest.

Conclusion: There was considerable sickness load amongst the toddlers. Morbidity can be reduced by improvement in the health care system at peripheral level.
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http://dx.doi.org/10.7860/JCDR/2014/8595.4706DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4190742PMC
August 2014

Plasticity and reprogramming of differentiated ocular tissue of tadpoles of the frog, Euphlyctis cyanophlyctis under the influence of vitamin A.

Indian J Exp Biol 2013 Jan;51(1):23-8

Department of Life Sciences, I A S E (D) University, GVM, Sardarshahr 331 401, India.

Present study has shown that differentiated cell types may loose their definitive characteristics and acquire features of another specialized cell type. Young (3 toe stage) and mature (5 toe stage) tadpoles of the frog, Euphylictis cyanophlyctis were employed as experimental animals. Experiments were completed in two phases: in the first part of experiment, lenses were extracted from right eye balls of tadpoles and treated with vitamin A; in the second part of the experiment, meshed lentectomized eye ball tissues were implanted into the pit made on mid lateral position of the tail of young and mature tadpoles and were treated with vitamin A. The results obtained gave clear evidence of plasticity and reprogramming of terminally differentiated ocular tissue into lens, retina and even complete eye. Vitamin A was found to be good model for accelerating the reprogramming of differentiated ocular tissue in anuran frog tadpoles.
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January 2013

Spectral, thermal and electrochemical investigation of carbohydrazone derived ionophore as Fe(III) ion selective electrode.

Spectrochim Acta A Mol Biomol Spectrosc 2013 Apr 1;107:271-9. Epub 2013 Feb 1.

Department of Chemistry, Zakir Husain Delhi College, University of Delhi, New Delhi 110 002, India.

Dibenzoylmethane bis(carbohydrazone) (BMBC) has been synthesized and structurally characterized on the basis of IR, (1)H NMR, mass, UV spectra and thermogravimetric analyses. BMBC has been analysed electrochemically and explored as new N, N Schiff base. It plays the role of an excellent ion carrier in the construction of iron(III) ion selective membrane sensor. This sensor shows very good selectivity and sensitivity towards iron ion over a wide variety of cations, including alkali, alkaline earth, transition and heavy metal ions. The response mechanism was discussed in the view of UV-spectroscopy and Electrochemical Impedance Spectroscopy (EIS). The proposed sensor was successfully used for the determination of iron in different samples.
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http://dx.doi.org/10.1016/j.saa.2013.01.055DOI Listing
April 2013

Development of transducer matrices based upon nanostructured conducting polymer for application in biosensors.

Indian J Exp Biol 2010 Oct;48(10):1053-62

Amity Institute of Biotechnology, Amity University Uttar Pradesh, Noida 201 303, India.

The nanostructured polyaniline (NSPANI) and its gold nano composite (GNP) with controlled size distribution were developed using structure directing agents (SDA). The nano structure of polyaniline were investigated by UV-Visible spectroscopy, transmission electron microscopy (TEM), dynamic light scattering (DLS), fourrier transform spectroscopy (FTIR), X-ray crystallography and scanning electron microscopy(SEM) etc. These characterization techniques reveal the spherical shape of polyaniline nanoparticles and size in the range of 7-50 nm depending on the type of dopant and nature of SDA. In general, these NSCP colloidal solutions are highly stable. UV-Visible spectra show mainly two peaks at 360-430 nm and at 780-870 nm. The bathochromic shift of the UV-Visible bands as compared to bulk polyaniline, reflect high DC conductivity. TEM and DLS results demonstrate the formation of nanostructure with narrow size distribution. Due to remarkable properties of, it is used as an efficient transduction matrice for the development of highly sensitive, reproducible, stable optical cholesterol and H202 biosensors having wide range of linearity and low Km values.
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October 2010

Psoriasis and cardiomyopathy: an intriguing association.

Indian J Dermatol 2010 Jul-Sep;55(3):271-3

Department of Medicine, Era's Lucknow Medical College & Hospital, Lucknow. U. P. India.

A 25-year-old male symptomatic of heart disease for four months presented with biventricular failure. Echocardiography revealed dilated cardiomyopathy. He had skin lesions for 10 years which were clinically and histopathologically identified as psoriasis. Association of cardiomyopathy with psoriasis is uncommon and intriguing. The link between dilated cardiomyopathy and psoriasis on a common inflammatory background is discussed.
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http://dx.doi.org/10.4103/0019-5154.70689DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2965917PMC
July 2011

Effect of prematurity and intrauterine growth restriction on H-reflex recovery cycle in neonates.

Neurosci Lett 2011 Jan 17;488(2):107-11. Epub 2010 Sep 17.

Department of Pediatrics, Unit of Neonatology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India. baldev

Objective: The purpose of this work was to assess the effects of prematurity and intrauterine growth restriction on spinal cord synapses using H-reflex.

Methods: 33 babies were investigated at birth. 14 were full term appropriate for gestational age (FT AGA), 10 were full term intrauterine growth restricted (FT IUGR) and 9 were preterm appropriate for gestational age (PT AGA). The maximum amplitude of H-reflex (Hmax), H-reflex latency (HRL), H/M ratio, H-reflex conduction velocity (HRCV), and H-reflex response to double stimuli (conditioning and test) for H-reflex recovery cycle (HRRC) were recorded in right lower limb (soleus muscle) in all the three groups.

Results: Percentage recovery values of H-reflex were significantly higher in FT AGA and FT IUGR babies compared to PT AGA neonates for most of inter-stimulus intervals. No significant differences were observed in H-reflex parameters between FT AGA and FT IUGR groups, but HRL and HRCV were significantly affected in PT AGA group.

Conclusions: Delayed H-reflex recovery in preterms may be due to a prolonged state of neurotransmitter delay in Ia terminals following initial activation by the conditioning stimuli. The cause of such prolonged depletion of neurotransmitters could be attributed to a poor neurotransmitter store in synaptic vesicles of spinal cord in preterm neonates.
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http://dx.doi.org/10.1016/j.neulet.2010.08.087DOI Listing
January 2011
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